CSPI04G18150 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G18150
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	proline-rich receptor-like protein kinase PERK2
Location	Chr4: 15545097 .. 15545450 (+)
RNA-Seq Expression	CSPI04G18150
Synteny	CSPI04G18150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTACTTCAAGGTTCTTGATCATTGCCTTTACGGCTACTTTGGCTCTCTCAAGCATCAATTTTGGCCATGCTGCTCGCCATCTTTCGCAAACCACTCCGCCCTTGGTGCCAAATCTTCCAGCAATTCCAACTCTGATACCGCCATTGCCTGGCTTGCCAAACCCTACTCTTCCAAAGTTACCACAACCGACATTACCGAAGATTAATTTTCCTCCACTTCCAATCGGGCTAGCGTTGCCACCGCTTCCGACAATGACACTTCCAAAGTTTCCACCAATCCCTACCATTCCAATTTCATTTCCTTCCATCCCATTTTTGTCCCCTCCTCCTGCGACATCTTCTTCCCCATAA

mRNA sequence

ATGGCTACTTCAAGGTTCTTGATCATTGCCTTTACGGCTACTTTGGCTCTCTCAAGCATCAATTTTGGCCATGCTGCTCGCCATCTTTCGCAAACCACTCCGCCCTTGGTGCCAAATCTTCCAGCAATTCCAACTCTGATACCGCCATTGCCTGGCTTGCCAAACCCTACTCTTCCAAAGTTACCACAACCGACATTACCGAAGATTAATTTTCCTCCACTTCCAATCGGGCTAGCGTTGCCACCGCTTCCGACAATGACACTTCCAAAGTTTCCACCAATCCCTACCATTCCAATTTCATTTCCTTCCATCCCATTTTTGTCCCCTCCTCCTGCGACATCTTCTTCCCCATAA

Coding sequence (CDS)

ATGGCTACTTCAAGGTTCTTGATCATTGCCTTTACGGCTACTTTGGCTCTCTCAAGCATCAATTTTGGCCATGCTGCTCGCCATCTTTCGCAAACCACTCCGCCCTTGGTGCCAAATCTTCCAGCAATTCCAACTCTGATACCGCCATTGCCTGGCTTGCCAAACCCTACTCTTCCAAAGTTACCACAACCGACATTACCGAAGATTAATTTTCCTCCACTTCCAATCGGGCTAGCGTTGCCACCGCTTCCGACAATGACACTTCCAAAGTTTCCACCAATCCCTACCATTCCAATTTCATTTCCTTCCATCCCATTTTTGTCCCCTCCTCCTGCGACATCTTCTTCCCCATAA

Protein sequence

MATSRFLIIAFTATLALSSINFGHAARHLSQTTPPLVPNLPAIPTLIPPLPGLPNPTLPKLPQPTLPKINFPPLPIGLALPPLPTMTLPKFPPIPTIPISFPSIPFLSPPPATSSSP*

Homology

[Close]

BLAST of CSPI04G18150 vs. ExPASy TrEMBL
Match: A0A0A0L1Y3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G374890 PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 4.7e-53
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 0


Query: 1   MATSRFLIIAFTATLALSSINFGHAARHLSQTTPPLVPNLPAIPTLIPPLPGLPNPTLPK 60
           MATSRFLIIAFTATLALSSINFGHAARHLSQTTPPLVPNLPAIPTLIPPLPGLPNPTLPK
Sbjct: 1   MATSRFLIIAFTATLALSSINFGHAARHLSQTTPPLVPNLPAIPTLIPPLPGLPNPTLPK 60

Query: 61  LPQPTLPKINFPPLPIGLALPPLPTMTLPKFPPIPTIPISFPSIPFLSPPPATSSSP 118
           LPQPTLPKINFPPLPIGLALPPLPTMTLPKFPPIPTIPISFPSIPFLSPPPATSSSP
Sbjct: 61  LPQPTLPKINFPPLPIGLALPPLPTMTLPKFPPIPTIPISFPSIPFLSPPPATSSSP 117

[Close]

BLAST of CSPI04G18150 vs. ExPASy TrEMBL
Match: A0A5D3C8R8 (Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold544G00410 PE=4 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 9.8e-43
Identity = 100/117 (85.47%), Postives = 104/117 (88.89%), Query Frame = 0


Query: 1   MATSRFLIIAFTATLALSSINFGHAARHLSQTTPPLVPNLPAIPTLIPPLPGLPNPTLPK 60
           MA+S+FLIIAF ATLALSSINFG AARHL QTTPPLVPNLPAIPT IPP P LPNPTLP 
Sbjct: 1   MASSKFLIIAFMATLALSSINFGQAARHLLQTTPPLVPNLPAIPTGIPPFPALPNPTLPN 60

Query: 61  LPQPTLPKINFPPLPIGLALPPLPTMTLPKFPPIPTIPISFPSIPFLSPPPATSSSP 118
           LPQPT+PK+N PPLP GLALPPLPTM LP FP IPTIP SFPSIPFLSPPPATSSSP
Sbjct: 61  LPQPTIPKVNLPPLPNGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSPPPATSSSP 117

[Close]

BLAST of CSPI04G18150 vs. ExPASy TrEMBL
Match: A0A1S3CJJ1 (proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo OX=3656 GN=LOC103501166 PE=4 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 9.8e-43
Identity = 100/117 (85.47%), Postives = 104/117 (88.89%), Query Frame = 0


Query: 1   MATSRFLIIAFTATLALSSINFGHAARHLSQTTPPLVPNLPAIPTLIPPLPGLPNPTLPK 60
           MA+S+FLIIAF ATLALSSINFG AARHL QTTPPLVPNLPAIPT IPP P LPNPTLP 
Sbjct: 1   MASSKFLIIAFMATLALSSINFGQAARHLLQTTPPLVPNLPAIPTGIPPFPALPNPTLPN 60

Query: 61  LPQPTLPKINFPPLPIGLALPPLPTMTLPKFPPIPTIPISFPSIPFLSPPPATSSSP 118
           LPQPT+PK+N PPLP GLALPPLPTM LP FP IPTIP SFPSIPFLSPPPATSSSP
Sbjct: 61  LPQPTIPKVNLPPLPNGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSPPPATSSSP 117

[Close]

BLAST of CSPI04G18150 vs. ExPASy TrEMBL
Match: A0A5A7ULD9 (Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold288G001670 PE=4 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 2.2e-42
Identity = 99/117 (84.62%), Postives = 104/117 (88.89%), Query Frame = 0


Query: 1   MATSRFLIIAFTATLALSSINFGHAARHLSQTTPPLVPNLPAIPTLIPPLPGLPNPTLPK 60
           MA+S+FLIIAF ATLALSSINFG AARHL QTTPPL+PNLPAIPT IPP P LPNPTLP 
Sbjct: 1   MASSKFLIIAFMATLALSSINFGQAARHLLQTTPPLMPNLPAIPTGIPPFPALPNPTLPN 60

Query: 61  LPQPTLPKINFPPLPIGLALPPLPTMTLPKFPPIPTIPISFPSIPFLSPPPATSSSP 118
           LPQPT+PK+N PPLP GLALPPLPTM LP FP IPTIP SFPSIPFLSPPPATSSSP
Sbjct: 61  LPQPTIPKVNLPPLPNGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSPPPATSSSP 117

[Close]

BLAST of CSPI04G18150 vs. ExPASy TrEMBL
Match: A0A5D3C6P2 (Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold544G00400 PE=4 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.6e-37
Identity = 96/124 (77.42%), Postives = 100/124 (80.65%), Query Frame = 0


Query: 1   MATSRFLIIAFTATLALSSINFGHAARHLSQTTPPLVPNLPAIPTLIPPLPGLPNPTLPK 60
           MA+S+FLIIAF ATLALSSI+ G AARHL QT PPLVPNLP IPT IPPLP L NPTLP 
Sbjct: 1   MASSKFLIIAFMATLALSSIDVGQAARHLLQTAPPLVPNLPVIPTGIPPLPALSNPTLPN 60

Query: 61  LPQPTLPKINFPPL-------PIGLALPPLPTMTLPKFPPIPTIPISFPSIPFLSPPPAT 118
           LPQPTLPK+N PPL       P GLALPPLP M LP FP IPTIP SFPSIPFLSPPPAT
Sbjct: 61  LPQPTLPKVNLPPLPSSPVNIPSGLALPPLPNMALPNFPSIPTIPTSFPSIPFLSPPPAT 120

[Close]

BLAST of CSPI04G18150 vs. NCBI nr
Match: KGN54827.2 (hypothetical protein Csa_012328 [Cucumis sativus])

HSP 1 Score: 209.9 bits (533), Expect = 1.2e-50
Identity = 113/117 (96.58%), Postives = 114/117 (97.44%), Query Frame = 0


Query: 1   MATSRFLIIAFTATLALSSINFGHAARHLSQTTPPLVPNLPAIPTLIPPLPGLPNPTLPK 60
           M   +FLIIAFTATLALSSINFGHAARHLSQTTPPLVPNLPAIPTLIPPLPGLPNPTLPK
Sbjct: 86  MTLPKFLIIAFTATLALSSINFGHAARHLSQTTPPLVPNLPAIPTLIPPLPGLPNPTLPK 145

Query: 61  LPQPTLPKINFPPLPIGLALPPLPTMTLPKFPPIPTIPISFPSIPFLSPPPATSSSP 118
           LPQPTLPKINFPPLPIGLALPPLPTMTLPKFPPIPTIPISFPSIPFLSPPPATSSSP
Sbjct: 146 LPQPTLPKINFPPLPIGLALPPLPTMTLPKFPPIPTIPISFPSIPFLSPPPATSSSP 202

[Close]

BLAST of CSPI04G18150 vs. NCBI nr
Match: XP_008462893.1 (PREDICTED: proline-rich receptor-like protein kinase PERK2 [Cucumis melo] >TYK07568.1 proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa])

HSP 1 Score: 182.6 bits (462), Expect = 2.0e-42
Identity = 100/117 (85.47%), Postives = 104/117 (88.89%), Query Frame = 0


Query: 1   MATSRFLIIAFTATLALSSINFGHAARHLSQTTPPLVPNLPAIPTLIPPLPGLPNPTLPK 60
           MA+S+FLIIAF ATLALSSINFG AARHL QTTPPLVPNLPAIPT IPP P LPNPTLP 
Sbjct: 1   MASSKFLIIAFMATLALSSINFGQAARHLLQTTPPLVPNLPAIPTGIPPFPALPNPTLPN 60

Query: 61  LPQPTLPKINFPPLPIGLALPPLPTMTLPKFPPIPTIPISFPSIPFLSPPPATSSSP 118
           LPQPT+PK+N PPLP GLALPPLPTM LP FP IPTIP SFPSIPFLSPPPATSSSP
Sbjct: 61  LPQPTIPKVNLPPLPNGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSPPPATSSSP 117

[Close]

BLAST of CSPI04G18150 vs. NCBI nr
Match: KAA0056642.1 (proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa])

HSP 1 Score: 181.4 bits (459), Expect = 4.5e-42
Identity = 99/117 (84.62%), Postives = 104/117 (88.89%), Query Frame = 0


Query: 1   MATSRFLIIAFTATLALSSINFGHAARHLSQTTPPLVPNLPAIPTLIPPLPGLPNPTLPK 60
           MA+S+FLIIAF ATLALSSINFG AARHL QTTPPL+PNLPAIPT IPP P LPNPTLP 
Sbjct: 1   MASSKFLIIAFMATLALSSINFGQAARHLLQTTPPLMPNLPAIPTGIPPFPALPNPTLPN 60

Query: 61  LPQPTLPKINFPPLPIGLALPPLPTMTLPKFPPIPTIPISFPSIPFLSPPPATSSSP 118
           LPQPT+PK+N PPLP GLALPPLPTM LP FP IPTIP SFPSIPFLSPPPATSSSP
Sbjct: 61  LPQPTIPKVNLPPLPNGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSPPPATSSSP 117

[Close]

BLAST of CSPI04G18150 vs. NCBI nr
Match: XP_038904303.1 (protein PELPK1-like [Benincasa hispida])

HSP 1 Score: 170.6 bits (431), Expect = 7.9e-39
Identity = 97/118 (82.20%), Postives = 101/118 (85.59%), Query Frame = 0


Query: 1   MATSRFLIIAFTATLALSSINFGHAARHLSQTTPPLVPNLPAIPTLIPPLPGLPNP-TLP 60
           M +S+FLIIAF ATLALSSI+ G AARHL QT PPLVPNLP IPT IPPLP LPNP TLP
Sbjct: 1   MGSSKFLIIAFMATLALSSIDLGQAARHLLQTAPPLVPNLPVIPTGIPPLPTLPNPTTLP 60

Query: 61  KLPQPTLPKINFPPLPIGLALPPLPTMTLPKFPPIPTIPISFPSIPFLSPPPATSSSP 118
            LPQPTLPK+N PPLP GLALPPLPTM LP FP IPTIP SFPSIPFLSPPPATSSSP
Sbjct: 61  NLPQPTLPKVNLPPLPSGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSPPPATSSSP 118

[Close]

BLAST of CSPI04G18150 vs. NCBI nr
Match: KAA0056641.1 (proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa] >TYK07567.1 proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa])

HSP 1 Score: 165.2 bits (417), Expect = 3.3e-37
Identity = 96/124 (77.42%), Postives = 100/124 (80.65%), Query Frame = 0


Query: 1   MATSRFLIIAFTATLALSSINFGHAARHLSQTTPPLVPNLPAIPTLIPPLPGLPNPTLPK 60
           MA+S+FLIIAF ATLALSSI+ G AARHL QT PPLVPNLP IPT IPPLP L NPTLP 
Sbjct: 1   MASSKFLIIAFMATLALSSIDVGQAARHLLQTAPPLVPNLPVIPTGIPPLPALSNPTLPN 60

Query: 61  LPQPTLPKINFPPL-------PIGLALPPLPTMTLPKFPPIPTIPISFPSIPFLSPPPAT 118
           LPQPTLPK+N PPL       P GLALPPLP M LP FP IPTIP SFPSIPFLSPPPAT
Sbjct: 61  LPQPTLPKVNLPPLPSSPVNIPSGLALPPLPNMALPNFPSIPTIPTSFPSIPFLSPPPAT 120

[Close]

BLAST of CSPI04G18150 vs. TAIR 10
Match: AT4G38080.1 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 52.0 bits (123), Expect = 3.8e-07
Identity = 53/121 (43.80%), Postives = 69/121 (57.02%), Query Frame = 0


Query: 7   LIIAFTATLALSSINFGHAARHLSQTTPPLVP---NLPAIP-TLIPPLPGLPNP---TLP 66
           L+IA T T   +S++   A RHL Q+TP   P     P +P T +PP+P LP P   TLP
Sbjct: 12  LVIALTFTSFFTSLS---AHRHLLQSTPVTQPPALTFPPLPKTTMPPVPSLPTPGQQTLP 71

Query: 67  KLPQPTLPK-INFPPLPIGLALPPLPTMTLPKFPPIPTI------PISFP-SIPFLSPPP 113
           + PQPTLP+    PP+P    +P LP    P  P IP I      P +FP +IPFL+PPP
Sbjct: 72  Q-PQPTLPQPTGLPPMP-STQIPSLPNQVQPTIPNIPQINFPSNFPFNFPFNIPFLTPPP 127

[Close]

BLAST of CSPI04G18150 vs. TAIR 10
Match: AT2G22510.1 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 44.3 bits (103), Expect = 8.0e-05
Identity = 45/121 (37.19%), Postives = 62/121 (51.24%), Query Frame = 0


Query: 7   LIIAFTATLALSSINFGHAARHLSQTTPPLVP----NLPAIPTL----IPPLPGLPNPT- 66
           L+IA T T   + ++   A+RHL Q+TP + P      P +PT      PP   LP PT 
Sbjct: 8   LVIALTFTSFFTGLS---ASRHLLQSTPAITPPVTTTFPPLPTTTMPPFPPSTSLPQPTA 67

Query: 67  LPKLPQPTLPKINFPPLPIGLALPPLPTMTLPKFP-PIP-----TIPISFPSIPFLSPPP 113
            P LP   +P +  P  PI   +P  P + +P FP  IP      +P S P+IPF +PPP
Sbjct: 68  FPPLPSSQIPSLPNPAQPIN--IPNFPQINIPNFPISIPNNFPFNLPTSIPTIPFFTPPP 123

[Close]

BLAST of CSPI04G18150 vs. TAIR 10
Match: AT5G09530.1 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 43.1 bits (100), Expect = 1.8e-04
Identity = 45/121 (37.19%), Postives = 56/121 (46.28%), Query Frame = 0


Query: 1   MATSRFLIIAFTATLALSSINFGHAARHLSQTTPPLVPNLPAIPTL-IPPLPGLPNPTLP 60
           + +S  LII   A LA     F   AR L +   P +P LP +P   +P LP  P P LP
Sbjct: 12  LLSSPLLIICLIALLA---DPFSVGARRLLEDPKPEIPKLPELPKFEVPKLPEFPKPELP 71

Query: 61  KLPQ--------------PTLPKINFPPLPIGLALPPLPTMTLPKFPPIPTIPI-SFPSI 106
           KLP+              P LPK+   P P    LP +P + LPKFP IP   +   P I
Sbjct: 72  KLPEFPKPELPKIPEIPKPELPKVPEIPKPEETKLPDIPKLELPKFPEIPKPELPKMPEI 129

[Close]

BLAST of CSPI04G18150 vs. TAIR 10
Match: AT5G15780.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 40.8 bits (94), Expect = 8.8e-04
Identity = 41/89 (46.07%), Postives = 53/89 (59.55%), Query Frame = 0


Query: 35  PLVPNLPAIPTLIPPLPGLPN-PTLPKLP----QPTLPKINFPPLPIGLALPPLPTM-TL 94
           PL+P++P  PTL PP P +P+ P+LP +P     PTLP I   P P    LPP+PT+ TL
Sbjct: 276 PLIPSIPT-PTL-PPNPLIPSPPSLPPIPLIPTPPTLPTIPLLPTPPTPTLPPIPTIPTL 335

Query: 95  PKFPPIPTIPISFPSIPFLSPPPATSSSP 118
           P  P +P +PI  P  P L PPP +   P
Sbjct: 336 PPLPVLPPVPIVNP--PSLPPPPPSFPVP 360

The following BLAST results are available for this feature:

BLAST of CSPI04G18150 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI04G18150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1Y3	4.7e-53	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G374890 PE=4 SV=1	[more]
A0A5D3C8R8	9.8e-43	85.47	Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3CJJ1	9.8e-43	85.47	proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A5A7ULD9	2.2e-42	84.62	Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A5D3C6P2	1.6e-37	77.42	Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1...	[more]

BLAST of CSPI04G18150 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN54827.2	1.2e-50	96.58	hypothetical protein Csa_012328 [Cucumis sativus]	[more]
XP_008462893.1	2.0e-42	85.47	PREDICTED: proline-rich receptor-like protein kinase PERK2 [Cucumis melo] >TYK07...	[more]
KAA0056642.1	4.5e-42	84.62	proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa]	[more]
XP_038904303.1	7.9e-39	82.20	protein PELPK1-like [Benincasa hispida]	[more]
KAA0056641.1	3.3e-37	77.42	proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa] >TYK0...	[more]

BLAST of CSPI04G18150 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G38080.1	3.8e-07	43.80	hydroxyproline-rich glycoprotein family protein	[more]
AT2G22510.1	8.0e-05	37.19	hydroxyproline-rich glycoprotein family protein	[more]
AT5G09530.1	1.8e-04	37.19	hydroxyproline-rich glycoprotein family protein	[more]
AT5G15780.1	8.8e-04	46.07	Pollen Ole e 1 allergen and extensin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33088:SF30	LEGUMINOSIN GROUP485 SECRETED PEPTIDE	coord: 1..115
IPR044659	Protein PELPK-like	PANTHER	PTHR33088	MUCIN-2	coord: 1..115

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G18150.1	CSPI04G18150.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0008270	zinc ion binding