CSPI04G17230 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G17230
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCytokinin dehydrogenase
LocationChr4: 14717879 .. 14720324 (-)
RNA-Seq ExpressionCSPI04G17230
SyntenyCSPI04G17230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACCTCTCCTTTTGCTTTGCTTCACAAAGAACAAAACAGTGAAGCACTCTCTACCTCTCTCTCTCACACACTCTTTTTTCTCTCCCTCTCTTTCTCTCTCAAAGCATACTCTCAAGGAAAGATGGAGTCACTACCTTCTGGATTCATCAAACAAACCATTATTTTCCTCAAGTTATACATAATTCTATATATCAGTTACATACCTGACAGTACCAACCGCTTATTTGCAGCCTCAACAATTCCTCCTCATTTTGGTTCCTCTGATATATCAACTTATGCACTCCAAACACTTGAATTGGATGGTCACCTTAGCTTTGACAATAATCATCAAGCAGCAACAGATTTTGGAAACCGATACCATTCACTCCCATTAGCTGTTTTGCATCCAAAATCCGTCGCTGATATTTCGAGCATGATAAACCATATTTTCAGAATGGGATATGCGTCGGACTTGACAGTTGCAGCTAGAGGCCATGGACATTCCATCCAAGGTCAAGCTCAAGCCTATAATGGTGTAGTTATAAGTATGGAATCATTTAAGGGGACAGAAATGAAAATAAATGCGGGGGAATTACCTTATGTGGATGTTTCAGCTGGAGAGCTGTGGATAAATATCTTGCATGAAACTCTTAAACAAGGGCTGGCACCAAAATCTTGGACAGATTATTTGCATCTTACTGTTGGAGGCACCTTGTCAAATGCTGGCATTAGTGGGCAGGCTTTCAGGCATGGACCCCAAATCAGTAATGTTTATCAGCTGGAAGTTGTCACAGGTACCTTCATACTGTTCATTTTATTCCTTACCAAAAGTTCTTTTTCTTTTTTTACCAAAATAGTGTAGCATGATATCATAACATATCATGTGACATCACTTGTCATTTCACAAAGAGGCAACTTTTTTCTTTCTTGGTAATGTTCTAGTTTAAAATGACAACAGTGACTACTTTCTATACACTATTCTAATTTTTTCATCTCGTTGATTTTAATAAAAAAAATGTTTTCAAGTGTATTGCTGAAACTGAATGTTTCAAGGCTTGGAGATCTTTTTTAACACAAATATAAAGTTTAGAAAGCCTGAAATCTGAAATTTTTGCAGGAAAAGGAGAGGTGGTTGTCTGTTCAGAGAATCAAAATGCAGACCTTTTCCATGCTGTTCTTGGTGGGCTTGGACAGTTTGGCATCATAACAAGAGCAAGAATTTCTTTGGAAAAGGCACCAAAAATGGTAAGTATATGTTCCATTTCACTACATCGGGGTACTGAAATTTTCCAGTGATGTTTCTTAGTTTGAAATACAATCCATCCTCTTTTTTTCAGGTAAAATGGATAAGAGTGTTGTACTCAGACTTTTCCAAATTTACAAGAGACCAAGAGTATCTGATATCATCCAACGATCAATTTGATTATATTGAAGGATTTGTACTTATTAATAGAACAGGTCTTCTCAATAACTGGAGATCCTCTTTTAATCCCAAAGAACCACTGCAAGCCAGTAAATTCAATTCAGATGGTAAAATCTTCTTCTGCTTGGAAATAGCAAAGTACTTCAACCCGGACGAAATCGACTTTATGAACAAGGTAAGAAATCATCAGAAGTTGTGCCATCTAAACAAAAACAACCCCATAAAATACTGCTTTCCTAAACTTCTTTTTCTTCTAATGCAGAAAGTTGAAAGCTTGATGTCAGAATTGAGCTATATTCCATCAACCCTCTTCACATCAGAAGTTACTTACCTGGATTTCCTCGATCGTGTACACGTCTCAGAGAACAAGCTCCGAGCGAAAGGTTTATGGGAAATTCCTCATCCATGGTTAAATCTACTGATTCCCAAAAGCCAAATACATGATTTTGCTCAAGAGGTCTTTGGAAACATCCTCAAGGATACCAGCAATGGACCCATCATAATCTACCCAGTTAACAAATCCAAGTAATTGTCTCATAACTTCTTCATTTTTAAGAAAAATTGCTTTTTATCATGTCCATAGTCTCATTCTTCATTACTTTTCATTGGTTTAGGTGGAATAACAGAACATCTTTGGTCACTCCAGATGAAGATATTTTCTATTTAGTGGCATTTTTATCTTCTGCAGTTCCATCTTCTGTAGGAACAGATTGCTTAGAACACATTTTAGAACAAAATCAAAGAATTTTGGATTACTGTATTGATTTTCTCCCTGGTTTTAAGCAATATCTCCCTCATTACAGCAACCAAGAAGAATGGAGATCTCACTTTGGGCCCAAGTGGCAGACTATCTTGCAAAGGAAATCAATCTATGATCCTTTGGCAATTCTTGCTCCTGGGCAGAGGATATTCCAAAAGGGTGTGATTCCCTTCTTATAATAGATGTATCAGAATCAGCAACACATAAAAAGTTCCTTGAAGAATGTGAAAGCTATGATGACAAGAAAACTGTAAATTAAATGAGAAATCACATTATGCTGTTAG

mRNA sequence

CACCTCTCCTTTTGCTTTGCTTCACAAAGAACAAAACAGTGAAGCACTCTCTACCTCTCTCTCTCACACACTCTTTTTTCTCTCCCTCTCTTTCTCTCTCAAAGCATACTCTCAAGGAAAGATGGAGTCACTACCTTCTGGATTCATCAAACAAACCATTATTTTCCTCAAGTTATACATAATTCTATATATCAGTTACATACCTGACAGTACCAACCGCTTATTTGCAGCCTCAACAATTCCTCCTCATTTTGGTTCCTCTGATATATCAACTTATGCACTCCAAACACTTGAATTGGATGGTCACCTTAGCTTTGACAATAATCATCAAGCAGCAACAGATTTTGGAAACCGATACCATTCACTCCCATTAGCTGTTTTGCATCCAAAATCCGTCGCTGATATTTCGAGCATGATAAACCATATTTTCAGAATGGGATATGCGTCGGACTTGACAGTTGCAGCTAGAGGCCATGGACATTCCATCCAAGGTCAAGCTCAAGCCTATAATGGTGTAGTTATAAGTATGGAATCATTTAAGGGGACAGAAATGAAAATAAATGCGGGGGAATTACCTTATGTGGATGTTTCAGCTGGAGAGCTGTGGATAAATATCTTGCATGAAACTCTTAAACAAGGGCTGGCACCAAAATCTTGGACAGATTATTTGCATCTTACTGTTGGAGGCACCTTGTCAAATGCTGGCATTAGTGGGCAGGCTTTCAGGCATGGACCCCAAATCAGTAATGTTTATCAGCTGGAAGTTGTCACAGGAAAAGGAGAGGTGGTTGTCTGTTCAGAGAATCAAAATGCAGACCTTTTCCATGCTGTTCTTGGTGGGCTTGGACAGTTTGGCATCATAACAAGAGCAAGAATTTCTTTGGAAAAGGCACCAAAAATGGTAAAATGGATAAGAGTGTTGTACTCAGACTTTTCCAAATTTACAAGAGACCAAGAGTATCTGATATCATCCAACGATCAATTTGATTATATTGAAGGATTTGTACTTATTAATAGAACAGGTCTTCTCAATAACTGGAGATCCTCTTTTAATCCCAAAGAACCACTGCAAGCCAGTAAATTCAATTCAGATGGTAAAATCTTCTTCTGCTTGGAAATAGCAAAGTACTTCAACCCGGACGAAATCGACTTTATGAACAAGAAAGTTGAAAGCTTGATGTCAGAATTGAGCTATATTCCATCAACCCTCTTCACATCAGAAGTTACTTACCTGGATTTCCTCGATCGTGTACACGTCTCAGAGAACAAGCTCCGAGCGAAAGGTTTATGGGAAATTCCTCATCCATGGTTAAATCTACTGATTCCCAAAAGCCAAATACATGATTTTGCTCAAGAGGTCTTTGGAAACATCCTCAAGGATACCAGCAATGGACCCATCATAATCTACCCAGTTAACAAATCCAAGTGGAATAACAGAACATCTTTGGTCACTCCAGATGAAGATATTTTCTATTTAGTGGCATTTTTATCTTCTGCAGTTCCATCTTCTGTAGGAACAGATTGCTTAGAACACATTTTAGAACAAAATCAAAGAATTTTGGATTACTGTATTGATTTTCTCCCTGGTTTTAAGCAATATCTCCCTCATTACAGCAACCAAGAAGAATGGAGATCTCACTTTGGGCCCAAGTGGCAGACTATCTTGCAAAGGAAATCAATCTATGATCCTTTGGCAATTCTTGCTCCTGGGCAGAGGATATTCCAAAAGGGTGTGATTCCCTTCTTATAATAGATGTATCAGAATCAGCAACACATAAAAAGTTCCTTGAAGAATGTGAAAGCTATGATGACAAGAAAACTGTAAATTAAATGAGAAATCACATTATGCTGTTAG

Coding sequence (CDS)

ATGGAGTCACTACCTTCTGGATTCATCAAACAAACCATTATTTTCCTCAAGTTATACATAATTCTATATATCAGTTACATACCTGACAGTACCAACCGCTTATTTGCAGCCTCAACAATTCCTCCTCATTTTGGTTCCTCTGATATATCAACTTATGCACTCCAAACACTTGAATTGGATGGTCACCTTAGCTTTGACAATAATCATCAAGCAGCAACAGATTTTGGAAACCGATACCATTCACTCCCATTAGCTGTTTTGCATCCAAAATCCGTCGCTGATATTTCGAGCATGATAAACCATATTTTCAGAATGGGATATGCGTCGGACTTGACAGTTGCAGCTAGAGGCCATGGACATTCCATCCAAGGTCAAGCTCAAGCCTATAATGGTGTAGTTATAAGTATGGAATCATTTAAGGGGACAGAAATGAAAATAAATGCGGGGGAATTACCTTATGTGGATGTTTCAGCTGGAGAGCTGTGGATAAATATCTTGCATGAAACTCTTAAACAAGGGCTGGCACCAAAATCTTGGACAGATTATTTGCATCTTACTGTTGGAGGCACCTTGTCAAATGCTGGCATTAGTGGGCAGGCTTTCAGGCATGGACCCCAAATCAGTAATGTTTATCAGCTGGAAGTTGTCACAGGAAAAGGAGAGGTGGTTGTCTGTTCAGAGAATCAAAATGCAGACCTTTTCCATGCTGTTCTTGGTGGGCTTGGACAGTTTGGCATCATAACAAGAGCAAGAATTTCTTTGGAAAAGGCACCAAAAATGGTAAAATGGATAAGAGTGTTGTACTCAGACTTTTCCAAATTTACAAGAGACCAAGAGTATCTGATATCATCCAACGATCAATTTGATTATATTGAAGGATTTGTACTTATTAATAGAACAGGTCTTCTCAATAACTGGAGATCCTCTTTTAATCCCAAAGAACCACTGCAAGCCAGTAAATTCAATTCAGATGGTAAAATCTTCTTCTGCTTGGAAATAGCAAAGTACTTCAACCCGGACGAAATCGACTTTATGAACAAGAAAGTTGAAAGCTTGATGTCAGAATTGAGCTATATTCCATCAACCCTCTTCACATCAGAAGTTACTTACCTGGATTTCCTCGATCGTGTACACGTCTCAGAGAACAAGCTCCGAGCGAAAGGTTTATGGGAAATTCCTCATCCATGGTTAAATCTACTGATTCCCAAAAGCCAAATACATGATTTTGCTCAAGAGGTCTTTGGAAACATCCTCAAGGATACCAGCAATGGACCCATCATAATCTACCCAGTTAACAAATCCAAGTGGAATAACAGAACATCTTTGGTCACTCCAGATGAAGATATTTTCTATTTAGTGGCATTTTTATCTTCTGCAGTTCCATCTTCTGTAGGAACAGATTGCTTAGAACACATTTTAGAACAAAATCAAAGAATTTTGGATTACTGTATTGATTTTCTCCCTGGTTTTAAGCAATATCTCCCTCATTACAGCAACCAAGAAGAATGGAGATCTCACTTTGGGCCCAAGTGGCAGACTATCTTGCAAAGGAAATCAATCTATGATCCTTTGGCAATTCTTGCTCCTGGGCAGAGGATATTCCAAAAGGGTGTGATTCCCTTCTTATAA

Protein sequence

MESLPSGFIKQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELDGHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGHSIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGGLGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVLINRTGLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIPSTLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKDTSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRILDYCIDFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGVIPFL*
Homology
BLAST of CSPI04G17230 vs. ExPASy Swiss-Prot
Match: O22213 (Cytokinin dehydrogenase 1 OS=Arabidopsis thaliana OX=3702 GN=CKX1 PE=1 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 2.2e-211
Identity = 351/526 (66.73%), Postives = 423/526 (80.42%), Query Frame = 0

Query: 15  FLKLYIILYISYIPDSTNRLFAASTIP----PHFGSSDISTYALQTLELDGHLSFDNNHQ 74
           FL +++IL +S IP  TN     S       P    SDI + +L +L+L+G++SFD+ H 
Sbjct: 17  FLGIFMILVLSCIPGRTNLCSNHSVSTPKELPSSNPSDIRS-SLVSLDLEGYISFDDVHN 76

Query: 75  AATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGHSIQGQAQAYN 134
            A DFGNRY   PLA+LHP+SV DISSM+ HI  +G  S+LTVAARGHGHS+QGQA A+ 
Sbjct: 77  VAKDFGNRYQLPPLAILHPRSVFDISSMMKHIVHLGSTSNLTVAARGHGHSLQGQALAHQ 136

Query: 135 GVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWTDYLHLTVGGT 194
           GVVI MES +  +++I  G+ PYVDVS GE+WINIL ETLK GL+PKSWTDYLHLTVGGT
Sbjct: 137 GVVIKMESLRSPDIRIYKGKQPYVDVSGGEIWINILRETLKYGLSPKSWTDYLHLTVGGT 196

Query: 195 LSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGGLGQFGIITRA 254
           LSNAGISGQAF+HGPQI+NVYQLE+VTGKGEVV CSE +N++LF +VLGGLGQFGIITRA
Sbjct: 197 LSNAGISGQAFKHGPQINNVYQLEIVTGKGEVVTCSEKRNSELFFSVLGGLGQFGIITRA 256

Query: 255 RISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVLINRTGLLNNWRSSF 314
           RISLE AP MVKWIRVLYSDFS F+RDQEYLIS    FDY+EGFV+INRT LLNNWRSSF
Sbjct: 257 RISLEPAPHMVKWIRVLYSDFSAFSRDQEYLISKEKTFDYVEGFVIINRTDLLNNWRSSF 316

Query: 315 NPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIPSTLFTSEVTY 374
           +P +  QAS+F SDGK  +CLE+ KYFNP+E   M+++   L+SEL+YIPSTLF+SEV Y
Sbjct: 317 SPNDSTQASRFKSDGKTLYCLEVVKYFNPEEASSMDQETGKLLSELNYIPSTLFSSEVPY 376

Query: 375 LDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKDTSNGPIIIYP 434
           ++FLDRVH++E KLRAKGLWE+PHPWLNLLIPKS I+ FA EVF NIL   +NGPI+IYP
Sbjct: 377 IEFLDRVHIAERKLRAKGLWEVPHPWLNLLIPKSSIYQFATEVFNNILTSNNNGPILIYP 436

Query: 435 VNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRILDYCIDFLPGF 494
           VN+SKW   TSL+TP+EDIFYLVAFL SAVP+S G + LE++L+QNQR++++C       
Sbjct: 437 VNQSKWKKHTSLITPNEDIFYLVAFLPSAVPNSSGKNDLEYLLKQNQRVMNFCAAANLNV 496

Query: 495 KQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQK 537
           KQYLPHY  Q+EW+SHFG +W+T  QRK  YDPLAILAPGQRIFQK
Sbjct: 497 KQYLPHYETQKEWKSHFGKRWETFAQRKQAYDPLAILAPGQRIFQK 541

BLAST of CSPI04G17230 vs. ExPASy Swiss-Prot
Match: Q75K78 (Cytokinin dehydrogenase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX9 PE=2 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 1.5e-199
Identity = 322/494 (65.18%), Postives = 400/494 (80.97%), Query Frame = 0

Query: 43  HFGSSDISTYALQTLELDGHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHI 102
           H    D+ + +L+ L LDGH SF +   AA DFGN     P+AVLHP SVADI++ I H+
Sbjct: 24  HVPKQDVPS-SLEELTLDGHFSFHDVSAAAQDFGNLSSFPPVAVLHPGSVADIATTIRHV 83

Query: 103 FRMGYASDLTVAARGHGHSIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELW 162
           F MG  S LTVAARGHGHS+ GQ+QA  G++ISMES +   M++N G  PYVD S GELW
Sbjct: 84  FLMGEHSTLTVAARGHGHSLYGQSQAAEGIIISMESLQSNTMRVNPGVSPYVDASGGELW 143

Query: 163 INILHETLKQGLAPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEV 222
           IN+LHETLK GLAPKSWTDYLHLTVGGTLSNAG+SGQ FRHGPQISNV +LE+VTG+G+V
Sbjct: 144 INVLHETLKYGLAPKSWTDYLHLTVGGTLSNAGVSGQTFRHGPQISNVNELEIVTGRGDV 203

Query: 223 VVCSENQNADLFHAVLGGLGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLI 282
           + CS  QN+DLFHA LGGLGQFG+ITRARI LE APKMV+W+RVLY DF+ FT DQE LI
Sbjct: 204 ITCSPEQNSDLFHAALGGLGQFGVITRARIPLEPAPKMVRWLRVLYLDFTSFTEDQEMLI 263

Query: 283 SSNDQFDYIEGFVLINRTGLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEI 342
           S+   FDYIEGFV+INRTG+LNNWRSSFNP++P+++S+F SDGK+ FCLE+ K FNPDE 
Sbjct: 264 SAEKTFDYIEGFVIINRTGILNNWRSSFNPQDPVRSSQFESDGKVLFCLEMTKNFNPDEA 323

Query: 343 DFMNKKVESLMSELSYIPSTLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIP 402
           D M ++V +L+S+L Y+PS+LF ++VTY++FLDRVH SE KLRAKG+WE+PHPWLN++IP
Sbjct: 324 DVMEQEVNTLLSQLRYMPSSLFHTDVTYIEFLDRVHSSEMKLRAKGMWEVPHPWLNIIIP 383

Query: 403 KSQIHDFAQEVFGNILKDTSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPS 462
           +S IH FA+EVFG ILKD++NGPI++YPVNKS+W+NRTS+V PDE++FYLVAFLSSA   
Sbjct: 384 RSMIHKFAKEVFGKILKDSNNGPILLYPVNKSRWDNRTSVVIPDEEVFYLVAFLSSA--- 443

Query: 463 SVGTDCLEHILEQNQRILDYCIDFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYD 522
            +G   ++H L+ N RI+++      G KQYLP+Y+ ++EW+SHFG +W T  QRK  YD
Sbjct: 444 -LGPHNIKHTLDLNYRIIEFSDKAGIGVKQYLPNYTTEQEWQSHFGARWDTFQQRKKAYD 503

Query: 523 PLAILAPGQRIFQK 537
           PLAILAPGQRIFQK
Sbjct: 504 PLAILAPGQRIFQK 512

BLAST of CSPI04G17230 vs. ExPASy Swiss-Prot
Match: Q9LY71 (Cytokinin dehydrogenase 6 OS=Arabidopsis thaliana OX=3702 GN=CKX6 PE=2 SV=2)

HSP 1 Score: 692.6 bits (1786), Expect = 3.6e-198
Identity = 340/532 (63.91%), Postives = 418/532 (78.57%), Query Frame = 0

Query: 10  KQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELDGHLSFDNNH 69
           K+T++ ++ + IL +S I       F          SS IS  +L+ L L GHL F++ H
Sbjct: 11  KRTMLIVRSFTILLLSCIAFKLACCF----------SSSIS--SLKALPLVGHLEFEHVH 70

Query: 70  QAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGHSIQGQAQAY 129
            A+ DFGNRY  +PLAVLHPKSV+DI+S I HI+ MG  S LTVAARG GHS+QGQAQ  
Sbjct: 71  HASKDFGNRYQLIPLAVLHPKSVSDIASTIRHIWMMGTHSQLTVAARGRGHSLQGQAQTR 130

Query: 130 NGVVISMESFKGTEMKINA--GELPYVDVSAGELWINILHETLKQGLAPKSWTDYLHLTV 189
           +G+VI MES    ++++ +     PYVDVS GELWINILHETLK GLAPKSWTDYLHLTV
Sbjct: 131 HGIVIHMESLHPQKLQVYSVDSPAPYVDVSGGELWINILHETLKYGLAPKSWTDYLHLTV 190

Query: 190 GGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGGLGQFGII 249
           GGTLSNAGISGQAFRHGPQISNV+QLE+VTGKGE++ C++ QN+DLF+ VLGGLGQFGII
Sbjct: 191 GGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEILNCTKRQNSDLFNGVLGGLGQFGII 250

Query: 250 TRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISS-NDQFDYIEGFVLINRTGLLNNW 309
           TRARI+LE AP MVKWIRVLY DF+ F +DQE LIS+   +FDYIEGFV+INRTGLLN+W
Sbjct: 251 TRARIALEPAPTMVKWIRVLYLDFAAFAKDQEQLISAQGHKFDYIEGFVIINRTGLLNSW 310

Query: 310 RSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIPSTLFTS 369
           R SF  +EPL+AS+F  DG+  +CLE+AKY   D  D +N++V+  +SELSY+ STLFT+
Sbjct: 311 RLSFTAEEPLEASQFKFDGRTLYCLELAKYLKQDNKDVINQEVKETLSELSYVTSTLFTT 370

Query: 370 EVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKDTSNGPI 429
           EV Y  FLDRVHVSE KLR+KG WE+PHPWLNLL+P+S+I++FA+ VFGNIL DTSNGP+
Sbjct: 371 EVAYEAFLDRVHVSEVKLRSKGQWEVPHPWLNLLVPRSKINEFARGVFGNILTDTSNGPV 430

Query: 430 IIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRILDYCIDF 489
           I+YPVNKSKW+N+TS VTP+E++FYLVA L+SA P S G D +E IL +N+RIL++  + 
Sbjct: 431 IVYPVNKSKWDNQTSAVTPEEEVFYLVAILTSASPGSAGKDGVEEILRRNRRILEFSEEA 490

Query: 490 LPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGV 539
             G KQYLPHY+ +EEWRSHFG KW   ++RKS YDPLAILAPG RIFQK V
Sbjct: 491 GIGLKQYLPHYTTREEWRSHFGDKWGEFVRRKSRYDPLAILAPGHRIFQKAV 530

BLAST of CSPI04G17230 vs. ExPASy Swiss-Prot
Match: Q5JLP4 (Cytokinin dehydrogenase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX4 PE=2 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 4.4e-196
Identity = 320/510 (62.75%), Postives = 398/510 (78.04%), Query Frame = 0

Query: 34  LFAASTIPPHFGSSDISTYALQTLELDGHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVA 93
           L A   +  H    D    +L  L LDGH SFD+ H AA DFGNR   LP AVLHP SV+
Sbjct: 18  LLALGGVTMHVPDEDDVVASLGALRLDGHFSFDDAHAAARDFGNRCSLLPAAVLHPGSVS 77

Query: 94  DISSMINHIFRMGYASDLTVAARGHGHSIQGQAQAYNGVVISMESFKGT---EMKINAGE 153
           D+++ +  +F++G +S LTVAARGHGHS+ GQ+QA  G+V+ MES        ++++ G 
Sbjct: 78  DVAATVRRVFQLGRSSPLTVAARGHGHSLLGQSQAAGGIVVKMESLAAAAARAVRVHGGA 137

Query: 154 LPYVDVSAGELWINILHETLKQGLAPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNV 213
            P+VD   GELWIN+LHETLK GLAP+SWTDYLHLTVGGTLSNAG+SGQAFRHGPQ+SNV
Sbjct: 138 SPHVDAPGGELWINVLHETLKHGLAPRSWTDYLHLTVGGTLSNAGVSGQAFRHGPQVSNV 197

Query: 214 YQLEVVTGKGEVVVCSENQNADLFHAVLGGLGQFGIITRARISLEKAPKMVKWIRVLYSD 273
            QLE+VTG+GEVV CS   N+DLF+A LGGLGQFGIITRARI+LE APKMV+WIRVLYSD
Sbjct: 198 NQLEIVTGRGEVVTCSHEVNSDLFYAALGGLGQFGIITRARIALEPAPKMVRWIRVLYSD 257

Query: 274 FSKFTRDQEYLISSNDQFDYIEGFVLINRTGLLNNWRSSFNPKEPLQASKFNSDGKIFFC 333
           F  FT DQE LI+S   FDYIEGFV+INRTG+LNNWR+SF P++P+QAS+F SDG++ +C
Sbjct: 258 FETFTEDQEKLIASEKTFDYIEGFVIINRTGILNNWRTSFKPQDPVQASQFQSDGRVLYC 317

Query: 334 LEIAKYFNPDEIDFMNKKVESLMSELSYIPSTLFTSEVTYLDFLDRVHVSENKLRAKGLW 393
           LE+   FN DE D M ++V +L+S L YI STLF ++VTYL+FLDRVH SE KLRA+GLW
Sbjct: 318 LELTMNFNHDEADIMEQEVGALLSRLRYISSTLFYTDVTYLEFLDRVHTSELKLRAQGLW 377

Query: 394 EIPHPWLNLLIPKSQIHDFAQEVFGNILKDTSNGPIIIYPVNKSKWNNRTSLVTPDEDIF 453
           E+PHPWLNLLIP+S +H FA+EVFG ILKD++NGPI++YPVN++KW+NRTS+V PDE+IF
Sbjct: 378 EVPHPWLNLLIPRSTVHKFAKEVFGKILKDSNNGPILLYPVNRTKWDNRTSVVIPDEEIF 437

Query: 454 YLVAFLSSAVPSSVGTDCLEHILEQNQRILDYCIDFLPGFKQYLPHYSNQEEWRSHFGPK 513
           YLV FLSSA PSS G   +EH +  N +I+D+C     G KQYL  Y+ Q++W++HFG +
Sbjct: 438 YLVGFLSSA-PSSSGHGSVEHAMNLNNKIVDFCEKNGVGMKQYLAPYTTQKQWKAHFGAR 497

Query: 514 WQTILQRKSIYDPLAILAPGQRIFQKGVIP 541
           W+T  +RK  YDPLAILAPGQRIF K  +P
Sbjct: 498 WETFERRKHTYDPLAILAPGQRIFPKASLP 526

BLAST of CSPI04G17230 vs. ExPASy Swiss-Prot
Match: Q67YU0 (Cytokinin dehydrogenase 5 OS=Arabidopsis thaliana OX=3702 GN=CKX5 PE=2 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 8.4e-155
Identity = 270/487 (55.44%), Postives = 359/487 (73.72%), Query Frame = 0

Query: 57  LELDGHLSFDNNHQA--ATDFGN-RYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTV 116
           +++DGH +   +  A  ++DFG  +    PLAVLHP S  D++ ++   +  G A+   V
Sbjct: 38  IDVDGHFTVHPSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAY--GSATAFPV 97

Query: 117 AARGHGHSIQGQAQA-YNGVVISM-ESFKGTEMKINAGELPYVDVSAGELWINILHETLK 176
           +ARGHGHSI GQA A  NGVV+ M     GT   +   +  YVDV  GELW+++L +TL+
Sbjct: 98  SARGHGHSINGQAAAGRNGVVVEMNHGVTGTPKPLVRPDEMYVDVWGGELWVDVLKKTLE 157

Query: 177 QGLAPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNA 236
            GLAPKSWTDYL+LTVGGTLSNAGISGQAF HGPQISNV +L+VVTGKGEV+ CSE +N 
Sbjct: 158 HGLAPKSWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTGKGEVMRCSEEENT 217

Query: 237 DLFHAVLGGLGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQ--FD 296
            LFH VLGGLGQFGIITRARISLE AP+ V+WIRVLYS F  FT DQEYLIS + Q  FD
Sbjct: 218 RLFHGVLGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLKFD 277

Query: 297 YIEGFVLINRTGLLNNWRSS-FNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKK 356
           Y+EGFV+++  GL+NNWRSS F+P+ P++ S  +S+G + +CLEI K ++  + + ++++
Sbjct: 278 YVEGFVIVDE-GLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDSDSEIVDQE 337

Query: 357 VESLMSELSYIPSTLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHD 416
           VE LM +L++IP+++FT+++ Y+DFLDRVH +E KLR+K LWE+PHPWLNL +PKS+I D
Sbjct: 338 VEILMKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRISD 397

Query: 417 FAQEVFGNILKDTSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDC 476
           F + VF  IL + ++GPI+IYP+NK KW+ R+S VTPDE++FYLVA L SA+     T  
Sbjct: 398 FDKGVFKGILGNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALTDGEETQK 457

Query: 477 LEHILEQNQRILDYCIDFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILA 536
           LE++ +QN+RIL++C       KQYLPH++ QEEW +HFG KW      K+ +DP  ILA
Sbjct: 458 LEYLKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEFDPRHILA 517

BLAST of CSPI04G17230 vs. ExPASy TrEMBL
Match: A0A0A0L1T0 (Cytokinin dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_4G343590 PE=3 SV=1)

HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 539/542 (99.45%), Postives = 542/542 (100.00%), Query Frame = 0

Query: 1   MESLPSGFIKQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 60
           MESLPSGFIKQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD
Sbjct: 1   MESLPSGFIKQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 60

Query: 61  GHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 120
           GHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH
Sbjct: 61  GHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 120

Query: 121 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180
           SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT
Sbjct: 121 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180

Query: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 240
           DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG
Sbjct: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 240

Query: 241 LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVLINRT 300
           LGQFGIITRARISLEKAPKMVKW+RVLYSDFSKFTRDQEYLISSND+FDYIEGFVLINRT
Sbjct: 241 LGQFGIITRARISLEKAPKMVKWMRVLYSDFSKFTRDQEYLISSNDRFDYIEGFVLINRT 300

Query: 301 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIP 360
           GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIP
Sbjct: 301 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIP 360

Query: 361 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKD 420
           STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKD
Sbjct: 361 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKD 420

Query: 421 TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRIL 480
           TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRIL
Sbjct: 421 TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRIL 480

Query: 481 DYCIDFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGVIP 540
           DYCI+FLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGVIP
Sbjct: 481 DYCIEFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGVIP 540

Query: 541 FL 543
           FL
Sbjct: 541 FL 542

BLAST of CSPI04G17230 vs. ExPASy TrEMBL
Match: A0A5A7VL02 (Cytokinin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold138G00470 PE=3 SV=1)

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 534/542 (98.52%), Postives = 539/542 (99.45%), Query Frame = 0

Query: 1   MESLPSGFIKQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 60
           MESLPSGFIKQTIIFL+LYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD
Sbjct: 1   MESLPSGFIKQTIIFLELYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 60

Query: 61  GHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 120
           G+LSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH
Sbjct: 61  GYLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 120

Query: 121 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180
           SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT
Sbjct: 121 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180

Query: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 240
           DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG
Sbjct: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 240

Query: 241 LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVLINRT 300
           LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFV INRT
Sbjct: 241 LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVFINRT 300

Query: 301 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIP 360
           GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLE+AKYFNPDEIDFMNKKVESLMSELSYIP
Sbjct: 301 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEMAKYFNPDEIDFMNKKVESLMSELSYIP 360

Query: 361 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKD 420
           STLFTSEVTYLDFLDRVHVSENKLRAKGLWE+PHPWLNLLIPKSQIHDFAQEVFGNILKD
Sbjct: 361 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEVPHPWLNLLIPKSQIHDFAQEVFGNILKD 420

Query: 421 TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRIL 480
           TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTD LEHILEQNQRIL
Sbjct: 421 TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDGLEHILEQNQRIL 480

Query: 481 DYCIDFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGVIP 540
           DYCIDFLPG KQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKG+IP
Sbjct: 481 DYCIDFLPGVKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGIIP 540

Query: 541 FL 543
           FL
Sbjct: 541 FL 542

BLAST of CSPI04G17230 vs. ExPASy TrEMBL
Match: A0A1S3CLV0 (Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103502439 PE=3 SV=1)

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 534/542 (98.52%), Postives = 539/542 (99.45%), Query Frame = 0

Query: 1   MESLPSGFIKQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 60
           MESLPSGFIKQTIIFL+LYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD
Sbjct: 1   MESLPSGFIKQTIIFLELYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 60

Query: 61  GHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 120
           G+LSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH
Sbjct: 61  GYLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 120

Query: 121 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180
           SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT
Sbjct: 121 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180

Query: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 240
           DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG
Sbjct: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 240

Query: 241 LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVLINRT 300
           LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFV INRT
Sbjct: 241 LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVFINRT 300

Query: 301 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIP 360
           GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLE+AKYFNPDEIDFMNKKVESLMSELSYIP
Sbjct: 301 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEMAKYFNPDEIDFMNKKVESLMSELSYIP 360

Query: 361 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKD 420
           STLFTSEVTYLDFLDRVHVSENKLRAKGLWE+PHPWLNLLIPKSQIHDFAQEVFGNILKD
Sbjct: 361 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEVPHPWLNLLIPKSQIHDFAQEVFGNILKD 420

Query: 421 TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRIL 480
           TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTD LEHILEQNQRIL
Sbjct: 421 TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDGLEHILEQNQRIL 480

Query: 481 DYCIDFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGVIP 540
           DYCIDFLPG KQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKG+IP
Sbjct: 481 DYCIDFLPGVKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGIIP 540

Query: 541 FL 543
           FL
Sbjct: 541 FL 542

BLAST of CSPI04G17230 vs. ExPASy TrEMBL
Match: A0A5D3BX61 (Cytokinin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold527G00570 PE=3 SV=1)

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 533/542 (98.34%), Postives = 538/542 (99.26%), Query Frame = 0

Query: 1   MESLPSGFIKQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 60
           MESLPSGFIKQTIIFL+LYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD
Sbjct: 1   MESLPSGFIKQTIIFLELYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 60

Query: 61  GHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 120
           G+LSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH
Sbjct: 61  GYLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 120

Query: 121 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180
           SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT
Sbjct: 121 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180

Query: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 240
           DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG
Sbjct: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 240

Query: 241 LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVLINRT 300
           LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFV INRT
Sbjct: 241 LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVFINRT 300

Query: 301 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIP 360
           GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLE+AKYFNPDEIDFMNKKVESLMSELSYIP
Sbjct: 301 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEMAKYFNPDEIDFMNKKVESLMSELSYIP 360

Query: 361 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKD 420
           STLFTSEVTYLDFLDRVHVSENKLRAKGLWE+PHPWLNLLIPKSQIHDFAQEVFGNILKD
Sbjct: 361 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEVPHPWLNLLIPKSQIHDFAQEVFGNILKD 420

Query: 421 TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRIL 480
           TSNGPIIIYPVNK KWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTD LEHILEQNQRIL
Sbjct: 421 TSNGPIIIYPVNKFKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDGLEHILEQNQRIL 480

Query: 481 DYCIDFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGVIP 540
           DYCIDFLPG KQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKG+IP
Sbjct: 481 DYCIDFLPGVKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGIIP 540

Query: 541 FL 543
           FL
Sbjct: 541 FL 542

BLAST of CSPI04G17230 vs. ExPASy TrEMBL
Match: A0A6J1EEA8 (Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111432444 PE=3 SV=1)

HSP 1 Score: 1015.8 bits (2625), Expect = 6.9e-293
Identity = 488/538 (90.71%), Postives = 518/538 (96.28%), Query Frame = 0

Query: 1   MESLPSGFIKQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 60
           M S PSGFIKQ+IIFLKLYIILYIS IPDSTN LFAA TIPPHFGSSDIS+Y LQTLELD
Sbjct: 1   MGSPPSGFIKQSIIFLKLYIILYISCIPDSTNHLFAAPTIPPHFGSSDISSYELQTLELD 60

Query: 61  GHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 120
           G+LSFDNNH+AATDFGNRY+  PLAVLHPKSV+DIS+ INHIF MGYASDLTVAARGHGH
Sbjct: 61  GYLSFDNNHKAATDFGNRYNLYPLAVLHPKSVSDISNTINHIFSMGYASDLTVAARGHGH 120

Query: 121 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180
           SIQGQAQAYNGVVI+MESF+G EMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT
Sbjct: 121 SIQGQAQAYNGVVINMESFQGLEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180

Query: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 240
           DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVV CSENQNADLFHAVLGG
Sbjct: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVACSENQNADLFHAVLGG 240

Query: 241 LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVLINRT 300
           LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQE+LISSNDQFDYIEGFV INRT
Sbjct: 241 LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEHLISSNDQFDYIEGFVFINRT 300

Query: 301 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIP 360
           GLLNNWRSSFNP+EPLQASKFNSDGK+F+CLEIAKYFNPDE+DF+NKKVESL++ LSYIP
Sbjct: 301 GLLNNWRSSFNPQEPLQASKFNSDGKVFYCLEIAKYFNPDELDFINKKVESLLTGLSYIP 360

Query: 361 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKD 420
           STLFTSEVTYL FLDRVHVSENKLRAKGLWEIPHPWLNLLIP++QIHDFAQEVFGNILKD
Sbjct: 361 STLFTSEVTYLAFLDRVHVSENKLRAKGLWEIPHPWLNLLIPRTQIHDFAQEVFGNILKD 420

Query: 421 TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRIL 480
           TSNGPIIIYPVNKSKWNN+TSLVTP+E++FYLVAFLSSAVPSS+GTD LEHIL QNQRIL
Sbjct: 421 TSNGPIIIYPVNKSKWNNKTSLVTPEEEVFYLVAFLSSAVPSSIGTDGLEHILAQNQRIL 480

Query: 481 DYCIDFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGV 539
           DYCI++LPG KQYLPHYS+Q EWR+HFGPKWQT+LQRKS YDPLAILAPGQRIFQKG+
Sbjct: 481 DYCIEYLPGVKQYLPHYSSQGEWRAHFGPKWQTVLQRKSKYDPLAILAPGQRIFQKGI 538

BLAST of CSPI04G17230 vs. NCBI nr
Match: XP_004142714.2 (cytokinin dehydrogenase 9 [Cucumis sativus])

HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 539/542 (99.45%), Postives = 542/542 (100.00%), Query Frame = 0

Query: 1   MESLPSGFIKQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 60
           MESLPSGFIKQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD
Sbjct: 65  MESLPSGFIKQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 124

Query: 61  GHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 120
           GHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH
Sbjct: 125 GHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 184

Query: 121 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180
           SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT
Sbjct: 185 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 244

Query: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 240
           DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG
Sbjct: 245 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 304

Query: 241 LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVLINRT 300
           LGQFGIITRARISLEKAPKMVKW+RVLYSDFSKFTRDQEYLISSND+FDYIEGFVLINRT
Sbjct: 305 LGQFGIITRARISLEKAPKMVKWMRVLYSDFSKFTRDQEYLISSNDRFDYIEGFVLINRT 364

Query: 301 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIP 360
           GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIP
Sbjct: 365 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIP 424

Query: 361 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKD 420
           STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKD
Sbjct: 425 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKD 484

Query: 421 TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRIL 480
           TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRIL
Sbjct: 485 TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRIL 544

Query: 481 DYCIDFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGVIP 540
           DYCI+FLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGVIP
Sbjct: 545 DYCIEFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGVIP 604

Query: 541 FL 543
           FL
Sbjct: 605 FL 606

BLAST of CSPI04G17230 vs. NCBI nr
Match: KAE8649608.1 (hypothetical protein Csa_012707 [Cucumis sativus])

HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 539/542 (99.45%), Postives = 542/542 (100.00%), Query Frame = 0

Query: 1   MESLPSGFIKQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 60
           MESLPSGFIKQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD
Sbjct: 158 MESLPSGFIKQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 217

Query: 61  GHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 120
           GHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH
Sbjct: 218 GHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 277

Query: 121 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180
           SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT
Sbjct: 278 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 337

Query: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 240
           DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG
Sbjct: 338 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 397

Query: 241 LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVLINRT 300
           LGQFGIITRARISLEKAPKMVKW+RVLYSDFSKFTRDQEYLISSND+FDYIEGFVLINRT
Sbjct: 398 LGQFGIITRARISLEKAPKMVKWMRVLYSDFSKFTRDQEYLISSNDRFDYIEGFVLINRT 457

Query: 301 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIP 360
           GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIP
Sbjct: 458 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIP 517

Query: 361 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKD 420
           STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKD
Sbjct: 518 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKD 577

Query: 421 TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRIL 480
           TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRIL
Sbjct: 578 TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRIL 637

Query: 481 DYCIDFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGVIP 540
           DYCI+FLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGVIP
Sbjct: 638 DYCIEFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGVIP 697

Query: 541 FL 543
           FL
Sbjct: 698 FL 699

BLAST of CSPI04G17230 vs. NCBI nr
Match: XP_008464594.1 (PREDICTED: cytokinin dehydrogenase 1 [Cucumis melo] >KAA0067890.1 cytokinin dehydrogenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 534/542 (98.52%), Postives = 539/542 (99.45%), Query Frame = 0

Query: 1   MESLPSGFIKQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 60
           MESLPSGFIKQTIIFL+LYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD
Sbjct: 1   MESLPSGFIKQTIIFLELYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 60

Query: 61  GHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 120
           G+LSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH
Sbjct: 61  GYLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 120

Query: 121 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180
           SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT
Sbjct: 121 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180

Query: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 240
           DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG
Sbjct: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 240

Query: 241 LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVLINRT 300
           LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFV INRT
Sbjct: 241 LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVFINRT 300

Query: 301 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIP 360
           GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLE+AKYFNPDEIDFMNKKVESLMSELSYIP
Sbjct: 301 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEMAKYFNPDEIDFMNKKVESLMSELSYIP 360

Query: 361 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKD 420
           STLFTSEVTYLDFLDRVHVSENKLRAKGLWE+PHPWLNLLIPKSQIHDFAQEVFGNILKD
Sbjct: 361 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEVPHPWLNLLIPKSQIHDFAQEVFGNILKD 420

Query: 421 TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRIL 480
           TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTD LEHILEQNQRIL
Sbjct: 421 TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDGLEHILEQNQRIL 480

Query: 481 DYCIDFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGVIP 540
           DYCIDFLPG KQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKG+IP
Sbjct: 481 DYCIDFLPGVKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGIIP 540

Query: 541 FL 543
           FL
Sbjct: 541 FL 542

BLAST of CSPI04G17230 vs. NCBI nr
Match: TYK04323.1 (cytokinin dehydrogenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 533/542 (98.34%), Postives = 538/542 (99.26%), Query Frame = 0

Query: 1   MESLPSGFIKQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 60
           MESLPSGFIKQTIIFL+LYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD
Sbjct: 1   MESLPSGFIKQTIIFLELYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 60

Query: 61  GHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 120
           G+LSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH
Sbjct: 61  GYLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 120

Query: 121 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180
           SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT
Sbjct: 121 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180

Query: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 240
           DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG
Sbjct: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 240

Query: 241 LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVLINRT 300
           LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFV INRT
Sbjct: 241 LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVFINRT 300

Query: 301 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIP 360
           GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLE+AKYFNPDEIDFMNKKVESLMSELSYIP
Sbjct: 301 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEMAKYFNPDEIDFMNKKVESLMSELSYIP 360

Query: 361 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKD 420
           STLFTSEVTYLDFLDRVHVSENKLRAKGLWE+PHPWLNLLIPKSQIHDFAQEVFGNILKD
Sbjct: 361 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEVPHPWLNLLIPKSQIHDFAQEVFGNILKD 420

Query: 421 TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRIL 480
           TSNGPIIIYPVNK KWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTD LEHILEQNQRIL
Sbjct: 421 TSNGPIIIYPVNKFKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDGLEHILEQNQRIL 480

Query: 481 DYCIDFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGVIP 540
           DYCIDFLPG KQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKG+IP
Sbjct: 481 DYCIDFLPGVKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGIIP 540

Query: 541 FL 543
           FL
Sbjct: 541 FL 542

BLAST of CSPI04G17230 vs. NCBI nr
Match: XP_038883660.1 (cytokinin dehydrogenase 1 [Benincasa hispida])

HSP 1 Score: 1072.8 bits (2773), Expect = 8.3e-310
Identity = 521/542 (96.13%), Postives = 532/542 (98.15%), Query Frame = 0

Query: 1   MESLPSGFIKQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELD 60
           MES PSGFIKQTIIFLKLYIILYISYIPDSTN LFAAS IPPHFGSSDISTYALQTLELD
Sbjct: 1   MESPPSGFIKQTIIFLKLYIILYISYIPDSTNHLFAASIIPPHFGSSDISTYALQTLELD 60

Query: 61  GHLSFDNNHQAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGH 120
           G+LSF+NNHQAATDFGNRYHSLPLAVLHPKSVADISS INHIFRMGYASDLTVAARGHGH
Sbjct: 61  GYLSFENNHQAATDFGNRYHSLPLAVLHPKSVADISSTINHIFRMGYASDLTVAARGHGH 120

Query: 121 SIQGQAQAYNGVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180
           SIQGQAQAYNGVVISMESFKG EMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT
Sbjct: 121 SIQGQAQAYNGVVISMESFKGAEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWT 180

Query: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 240
           DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG
Sbjct: 181 DYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGG 240

Query: 241 LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVLINRT 300
           LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISS+DQFDYIEGFV INRT
Sbjct: 241 LGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSSDQFDYIEGFVFINRT 300

Query: 301 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIP 360
           GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNK+VESL++ELSYIP
Sbjct: 301 GLLNNWRSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKQVESLLAELSYIP 360

Query: 361 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKD 420
           STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIP+SQIHDFA EVFGNILKD
Sbjct: 361 STLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPRSQIHDFANEVFGNILKD 420

Query: 421 TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRIL 480
           TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSS+GTD LEHILEQNQRIL
Sbjct: 421 TSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSLGTDGLEHILEQNQRIL 480

Query: 481 DYCIDFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGVIP 540
           DYCIDFLPG KQYLPHYS Q+EWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQ+G+IP
Sbjct: 481 DYCIDFLPGVKQYLPHYSTQDEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQRGIIP 540

Query: 541 FL 543
           FL
Sbjct: 541 FL 542

BLAST of CSPI04G17230 vs. TAIR 10
Match: AT2G41510.1 (cytokinin oxidase/dehydrogenase 1 )

HSP 1 Score: 736.5 bits (1900), Expect = 1.6e-212
Identity = 351/526 (66.73%), Postives = 423/526 (80.42%), Query Frame = 0

Query: 15  FLKLYIILYISYIPDSTNRLFAASTIP----PHFGSSDISTYALQTLELDGHLSFDNNHQ 74
           FL +++IL +S IP  TN     S       P    SDI + +L +L+L+G++SFD+ H 
Sbjct: 17  FLGIFMILVLSCIPGRTNLCSNHSVSTPKELPSSNPSDIRS-SLVSLDLEGYISFDDVHN 76

Query: 75  AATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGHSIQGQAQAYN 134
            A DFGNRY   PLA+LHP+SV DISSM+ HI  +G  S+LTVAARGHGHS+QGQA A+ 
Sbjct: 77  VAKDFGNRYQLPPLAILHPRSVFDISSMMKHIVHLGSTSNLTVAARGHGHSLQGQALAHQ 136

Query: 135 GVVISMESFKGTEMKINAGELPYVDVSAGELWINILHETLKQGLAPKSWTDYLHLTVGGT 194
           GVVI MES +  +++I  G+ PYVDVS GE+WINIL ETLK GL+PKSWTDYLHLTVGGT
Sbjct: 137 GVVIKMESLRSPDIRIYKGKQPYVDVSGGEIWINILRETLKYGLSPKSWTDYLHLTVGGT 196

Query: 195 LSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGGLGQFGIITRA 254
           LSNAGISGQAF+HGPQI+NVYQLE+VTGKGEVV CSE +N++LF +VLGGLGQFGIITRA
Sbjct: 197 LSNAGISGQAFKHGPQINNVYQLEIVTGKGEVVTCSEKRNSELFFSVLGGLGQFGIITRA 256

Query: 255 RISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVLINRTGLLNNWRSSF 314
           RISLE AP MVKWIRVLYSDFS F+RDQEYLIS    FDY+EGFV+INRT LLNNWRSSF
Sbjct: 257 RISLEPAPHMVKWIRVLYSDFSAFSRDQEYLISKEKTFDYVEGFVIINRTDLLNNWRSSF 316

Query: 315 NPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIPSTLFTSEVTY 374
           +P +  QAS+F SDGK  +CLE+ KYFNP+E   M+++   L+SEL+YIPSTLF+SEV Y
Sbjct: 317 SPNDSTQASRFKSDGKTLYCLEVVKYFNPEEASSMDQETGKLLSELNYIPSTLFSSEVPY 376

Query: 375 LDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKDTSNGPIIIYP 434
           ++FLDRVH++E KLRAKGLWE+PHPWLNLLIPKS I+ FA EVF NIL   +NGPI+IYP
Sbjct: 377 IEFLDRVHIAERKLRAKGLWEVPHPWLNLLIPKSSIYQFATEVFNNILTSNNNGPILIYP 436

Query: 435 VNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRILDYCIDFLPGF 494
           VN+SKW   TSL+TP+EDIFYLVAFL SAVP+S G + LE++L+QNQR++++C       
Sbjct: 437 VNQSKWKKHTSLITPNEDIFYLVAFLPSAVPNSSGKNDLEYLLKQNQRVMNFCAAANLNV 496

Query: 495 KQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQK 537
           KQYLPHY  Q+EW+SHFG +W+T  QRK  YDPLAILAPGQRIFQK
Sbjct: 497 KQYLPHYETQKEWKSHFGKRWETFAQRKQAYDPLAILAPGQRIFQK 541

BLAST of CSPI04G17230 vs. TAIR 10
Match: AT3G63440.1 (cytokinin oxidase/dehydrogenase 6 )

HSP 1 Score: 692.6 bits (1786), Expect = 2.6e-199
Identity = 340/532 (63.91%), Postives = 418/532 (78.57%), Query Frame = 0

Query: 10  KQTIIFLKLYIILYISYIPDSTNRLFAASTIPPHFGSSDISTYALQTLELDGHLSFDNNH 69
           K+T++ ++ + IL +S I       F          SS IS  +L+ L L GHL F++ H
Sbjct: 11  KRTMLIVRSFTILLLSCIAFKLACCF----------SSSIS--SLKALPLVGHLEFEHVH 70

Query: 70  QAATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTVAARGHGHSIQGQAQAY 129
            A+ DFGNRY  +PLAVLHPKSV+DI+S I HI+ MG  S LTVAARG GHS+QGQAQ  
Sbjct: 71  HASKDFGNRYQLIPLAVLHPKSVSDIASTIRHIWMMGTHSQLTVAARGRGHSLQGQAQTR 130

Query: 130 NGVVISMESFKGTEMKINA--GELPYVDVSAGELWINILHETLKQGLAPKSWTDYLHLTV 189
           +G+VI MES    ++++ +     PYVDVS GELWINILHETLK GLAPKSWTDYLHLTV
Sbjct: 131 HGIVIHMESLHPQKLQVYSVDSPAPYVDVSGGELWINILHETLKYGLAPKSWTDYLHLTV 190

Query: 190 GGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGGLGQFGII 249
           GGTLSNAGISGQAFRHGPQISNV+QLE+VTGKGE++ C++ QN+DLF+ VLGGLGQFGII
Sbjct: 191 GGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEILNCTKRQNSDLFNGVLGGLGQFGII 250

Query: 250 TRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISS-NDQFDYIEGFVLINRTGLLNNW 309
           TRARI+LE AP MVKWIRVLY DF+ F +DQE LIS+   +FDYIEGFV+INRTGLLN+W
Sbjct: 251 TRARIALEPAPTMVKWIRVLYLDFAAFAKDQEQLISAQGHKFDYIEGFVIINRTGLLNSW 310

Query: 310 RSSFNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIPSTLFTS 369
           R SF  +EPL+AS+F  DG+  +CLE+AKY   D  D +N++V+  +SELSY+ STLFT+
Sbjct: 311 RLSFTAEEPLEASQFKFDGRTLYCLELAKYLKQDNKDVINQEVKETLSELSYVTSTLFTT 370

Query: 370 EVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNILKDTSNGPI 429
           EV Y  FLDRVHVSE KLR+KG WE+PHPWLNLL+P+S+I++FA+ VFGNIL DTSNGP+
Sbjct: 371 EVAYEAFLDRVHVSEVKLRSKGQWEVPHPWLNLLVPRSKINEFARGVFGNILTDTSNGPV 430

Query: 430 IIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRILDYCIDF 489
           I+YPVNKSKW+N+TS VTP+E++FYLVA L+SA P S G D +E IL +N+RIL++  + 
Sbjct: 431 IVYPVNKSKWDNQTSAVTPEEEVFYLVAILTSASPGSAGKDGVEEILRRNRRILEFSEEA 490

Query: 490 LPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQKGV 539
             G KQYLPHY+ +EEWRSHFG KW   ++RKS YDPLAILAPG RIFQK V
Sbjct: 491 GIGLKQYLPHYTTREEWRSHFGDKWGEFVRRKSRYDPLAILAPGHRIFQKAV 530

BLAST of CSPI04G17230 vs. TAIR 10
Match: AT1G75450.1 (cytokinin oxidase 5 )

HSP 1 Score: 548.5 bits (1412), Expect = 6.0e-156
Identity = 270/487 (55.44%), Postives = 359/487 (73.72%), Query Frame = 0

Query: 57  LELDGHLSFDNNHQA--ATDFGN-RYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTV 116
           +++DGH +   +  A  ++DFG  +    PLAVLHP S  D++ ++   +  G A+   V
Sbjct: 38  IDVDGHFTVHPSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAY--GSATAFPV 97

Query: 117 AARGHGHSIQGQAQA-YNGVVISM-ESFKGTEMKINAGELPYVDVSAGELWINILHETLK 176
           +ARGHGHSI GQA A  NGVV+ M     GT   +   +  YVDV  GELW+++L +TL+
Sbjct: 98  SARGHGHSINGQAAAGRNGVVVEMNHGVTGTPKPLVRPDEMYVDVWGGELWVDVLKKTLE 157

Query: 177 QGLAPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNA 236
            GLAPKSWTDYL+LTVGGTLSNAGISGQAF HGPQISNV +L+VVTGKGEV+ CSE +N 
Sbjct: 158 HGLAPKSWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTGKGEVMRCSEEENT 217

Query: 237 DLFHAVLGGLGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQ--FD 296
            LFH VLGGLGQFGIITRARISLE AP+ V+WIRVLYS F  FT DQEYLIS + Q  FD
Sbjct: 218 RLFHGVLGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLKFD 277

Query: 297 YIEGFVLINRTGLLNNWRSS-FNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKK 356
           Y+EGFV+++  GL+NNWRSS F+P+ P++ S  +S+G + +CLEI K ++  + + ++++
Sbjct: 278 YVEGFVIVDE-GLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDSDSEIVDQE 337

Query: 357 VESLMSELSYIPSTLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHD 416
           VE LM +L++IP+++FT+++ Y+DFLDRVH +E KLR+K LWE+PHPWLNL +PKS+I D
Sbjct: 338 VEILMKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRISD 397

Query: 417 FAQEVFGNILKDTSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDC 476
           F + VF  IL + ++GPI+IYP+NK KW+ R+S VTPDE++FYLVA L SA+     T  
Sbjct: 398 FDKGVFKGILGNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALTDGEETQK 457

Query: 477 LEHILEQNQRILDYCIDFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILA 536
           LE++ +QN+RIL++C       KQYLPH++ QEEW +HFG KW      K+ +DP  ILA
Sbjct: 458 LEYLKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEFDPRHILA 517

BLAST of CSPI04G17230 vs. TAIR 10
Match: AT1G75450.2 (cytokinin oxidase 5 )

HSP 1 Score: 543.9 bits (1400), Expect = 1.5e-154
Identity = 270/488 (55.33%), Postives = 359/488 (73.57%), Query Frame = 0

Query: 57  LELDGHLSFDNNHQA--ATDFGN-RYHSLPLAVLHPKSVADISSMINHIFRMGYASDLTV 116
           +++DGH +   +  A  ++DFG  +    PLAVLHP S  D++ ++   +  G A+   V
Sbjct: 34  IDVDGHFTVHPSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAY--GSATAFPV 93

Query: 117 AARGHGHSIQGQAQA-YNGVVISM-ESFKGTEMKINAGELPYVDVSAGELWINILHETLK 176
           +ARGHGHSI GQA A  NGVV+ M     GT   +   +  YVDV  GELW+++L +TL+
Sbjct: 94  SARGHGHSINGQAAAGRNGVVVEMNHGVTGTPKPLVRPDEMYVDVWGGELWVDVLKKTLE 153

Query: 177 QGLAPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVYQLEVVT-GKGEVVVCSENQN 236
            GLAPKSWTDYL+LTVGGTLSNAGISGQAF HGPQISNV +L+VVT GKGEV+ CSE +N
Sbjct: 154 HGLAPKSWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTVGKGEVMRCSEEEN 213

Query: 237 ADLFHAVLGGLGQFGIITRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQ--F 296
             LFH VLGGLGQFGIITRARISLE AP+ V+WIRVLYS F  FT DQEYLIS + Q  F
Sbjct: 214 TRLFHGVLGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLKF 273

Query: 297 DYIEGFVLINRTGLLNNWRSS-FNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNK 356
           DY+EGFV+++  GL+NNWRSS F+P+ P++ S  +S+G + +CLEI K ++  + + +++
Sbjct: 274 DYVEGFVIVDE-GLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDSDSEIVDQ 333

Query: 357 KVESLMSELSYIPSTLFTSEVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIH 416
           +VE LM +L++IP+++FT+++ Y+DFLDRVH +E KLR+K LWE+PHPWLNL +PKS+I 
Sbjct: 334 EVEILMKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRIS 393

Query: 417 DFAQEVFGNILKDTSNGPIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTD 476
           DF + VF  IL + ++GPI+IYP+NK KW+ R+S VTPDE++FYLVA L SA+     T 
Sbjct: 394 DFDKGVFKGILGNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALTDGEETQ 453

Query: 477 CLEHILEQNQRILDYCIDFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAIL 536
            LE++ +QN+RIL++C       KQYLPH++ QEEW +HFG KW      K+ +DP  IL
Sbjct: 454 KLEYLKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEFDPRHIL 513

BLAST of CSPI04G17230 vs. TAIR 10
Match: AT5G56970.1 (cytokinin oxidase 3 )

HSP 1 Score: 474.9 bits (1221), Expect = 8.4e-134
Identity = 232/472 (49.15%), Postives = 322/472 (68.22%), Query Frame = 0

Query: 71  AATDFGNRYHSLPLAVLHPKSVADISSMINHIFRMGYASDLT--VAARGHGHSIQGQAQA 130
           AATDFG+     P AVL P SV DI+ +I    ++ + S L+  +AARGHGHS +GQA A
Sbjct: 58  AATDFGHVTKIFPSAVLIPSSVEDITDLI----KLSFDSQLSFPLAARGHGHSHRGQASA 117

Query: 131 YNGVVISMESFKGTEMKINAGE-LPYVDVSAGELWINILHETLKQGLAPKSWTDYLHLTV 190
            +GVV++M S    +  I       YVDV A  LWI +L++TL+ GL P SWTDYL+LTV
Sbjct: 118 KDGVVVNMRSMVNRDRGIKVSRTCLYVDVDAAWLWIEVLNKTLELGLTPVSWTDYLYLTV 177

Query: 191 GGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVVCSENQNADLFHAVLGGLGQFGII 250
           GGTLSN GISGQ FR+GPQI+NV +++V+TGKGE+  CS++ N+DLF AVLGGLGQFGII
Sbjct: 178 GGTLSNGGISGQTFRYGPQITNVLEMDVITGKGEIATCSKDMNSDLFFAVLGGLGQFGII 237

Query: 251 TRARISLEKAPKMVKWIRVLYSDFSKFTRDQEYLISSNDQFDYIEGFVLINRTGLLNNWR 310
           TRARI LE APK  KW+R LY DFS+FTRDQE +IS  D  D++EG ++++  G  +NWR
Sbjct: 238 TRARIKLEVAPKRAKWLRFLYIDFSEFTRDQERVISKTDGVDFLEGSIMVDH-GPPDNWR 297

Query: 311 SS-FNPKEPLQASKFNSDGKIFFCLEIAKYFNPDEIDFMNKKVESLMSELSYIPSTLFTS 370
           S+ + P + L+ +      ++ +CLE+ KY++      +N+++E L   L+++   ++  
Sbjct: 298 STYYPPSDHLRIASMVKRHRVIYCLEVVKYYDETSQYTVNEEMEELSDSLNHVRGFMYEK 357

Query: 371 EVTYLDFLDRVHVSENKLRAKGLWEIPHPWLNLLIPKSQIHDFAQEVFGNIL--KDTSNG 430
           +VTY+DFL+RV   E  L++KG W++PHPWLNL +PK+QI  F   VF  I+   + ++G
Sbjct: 358 DVTYMDFLNRVRTGELNLKSKGQWDVPHPWLNLFVPKTQISKFDDGVFKGIILRNNITSG 417

Query: 431 PIIIYPVNKSKWNNRTSLVTPDEDIFYLVAFLSSAVPSSVGTDCLEHILEQNQRILDYCI 490
           P+++YP+N++KWN+R S   P+ED+FY V FL SA     G D  E   ++N  IL +C 
Sbjct: 418 PVLVYPMNRNKWNDRMSAAIPEEDVFYAVGFLRSA-----GFDNWEAFDQENMEILKFCE 477

Query: 491 DFLPGFKQYLPHYSNQEEWRSHFGPKWQTILQRKSIYDPLAILAPGQRIFQK 537
           D   G  QYLP++S+QE W  HFGP+W   ++RK  YDP  IL+PGQ IFQK
Sbjct: 478 DANMGVIQYLPYHSSQEGWVRHFGPRWNIFVERKYKYDPKMILSPGQNIFQK 519

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O222132.2e-21166.73Cytokinin dehydrogenase 1 OS=Arabidopsis thaliana OX=3702 GN=CKX1 PE=1 SV=1[more]
Q75K781.5e-19965.18Cytokinin dehydrogenase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX9 PE=2 ... [more]
Q9LY713.6e-19863.91Cytokinin dehydrogenase 6 OS=Arabidopsis thaliana OX=3702 GN=CKX6 PE=2 SV=2[more]
Q5JLP44.4e-19662.75Cytokinin dehydrogenase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX4 PE=2 ... [more]
Q67YU08.4e-15555.44Cytokinin dehydrogenase 5 OS=Arabidopsis thaliana OX=3702 GN=CKX5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L1T00.0e+0099.45Cytokinin dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_4G343590 PE=3 SV=1[more]
A0A5A7VL020.0e+0098.52Cytokinin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A1S3CLV00.0e+0098.52Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103502439 PE=3 SV=1[more]
A0A5D3BX610.0e+0098.34Cytokinin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A6J1EEA86.9e-29390.71Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111432444 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004142714.20.0e+0099.45cytokinin dehydrogenase 9 [Cucumis sativus][more]
KAE8649608.10.0e+0099.45hypothetical protein Csa_012707 [Cucumis sativus][more]
XP_008464594.10.0e+0098.52PREDICTED: cytokinin dehydrogenase 1 [Cucumis melo] >KAA0067890.1 cytokinin dehy... [more]
TYK04323.10.0e+0098.34cytokinin dehydrogenase 1 [Cucumis melo var. makuwa][more]
XP_038883660.18.3e-31096.13cytokinin dehydrogenase 1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G41510.11.6e-21266.73cytokinin oxidase/dehydrogenase 1 [more]
AT3G63440.12.6e-19963.91cytokinin oxidase/dehydrogenase 6 [more]
AT1G75450.16.0e-15655.44cytokinin oxidase 5 [more]
AT1G75450.21.5e-15455.33cytokinin oxidase 5 [more]
AT5G56970.18.4e-13449.15cytokinin oxidase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 83..226
e-value: 4.3E-19
score: 68.6
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 136..259
e-value: 1.2E-216
score: 721.8
IPR016170Cytokinin dehydrogenase, C-terminal domain superfamilyGENE3D3.40.462.10coord: 260..493
e-value: 1.2E-216
score: 721.8
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 68..534
e-value: 1.2E-216
score: 721.8
IPR015345Cytokinin dehydrogenase 1, FAD/cytokinin binding domainPFAMPF09265Cytokin-bindcoord: 258..534
e-value: 5.0E-115
score: 383.7
NoneNo IPR availablePANTHERPTHR13878:SF120CYTOKININ DEHYDROGENASE 1-LIKE ISOFORM X1coord: 12..537
NoneNo IPR availablePANTHERPTHR13878GULONOLACTONE OXIDASEcoord: 12..537
IPR006093Oxygen oxidoreductase covalent FAD-binding sitePROSITEPS00862OX2_COVAL_FADcoord: 83..118
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 79..257
score: 14.772018
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 68..255
IPR016164FAD-linked oxidase-like, C-terminalSUPERFAMILY55103FAD-linked oxidases, C-terminal domaincoord: 258..534

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G17230.1CSPI04G17230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009690 cytokinin metabolic process
molecular_function GO:0019139 cytokinin dehydrogenase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity