CSPI04G17190 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G17190
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionEf-hand calcium binding protein
LocationChr4: 14645941 .. 14649113 (+)
RNA-Seq ExpressionCSPI04G17190
SyntenyCSPI04G17190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAACACGTTATTTCTACTTGGGATCAGCAATTTCAATTTTTTTTTTCCTGAAAATGTCAGGTTACCCAAATCAACCATCAGGTTACCCAAATCAACCATCAGGTTACGGTTACGGCTACGGCGGCACCGGTGGCTACGGAGCTCCACCACCGACTGCTCAGCCTTACAATTCTTCAGCTTACGGACATCCACAGTCCCAGACTCAGCAGCCGTACGCTCCGGTGGCCGCGCCATATGGCGTACCGTCCGCACCGTACGGGTCAGCTCAGGGCGAAAAACCGCCCAAGGACAAGCCTAATCAGTCGTACGGCGGTGCCGGTGGTGGTCATCAGTCTTACGGCGGAGCAGCCAGTGGTGGTCATGGTTATCCACCTCCTTCTGCTTATGGAAGCCCTTTTGCTTCACTCTTGCCATCGGCTTTTCCGCCTGGTACTGATCCGAGCATCGTTGCTTGTTTTCAAGTAGCCGATCAGGATGGTAGTGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTATCATCGTATAATCAGAAGTTTAGCATCAGAACTGTTCATCTTCTTATGTACCATTTCACCAATACGAATACCAGGAAGATCGGTAATCTCACTTTTCCCTCTATTGACTTTTTGTTTTCTCTGGAAATAAGACATTTCTTGTTGTTTTCTTTAAGTTTCTGAAATCAACAACAGTAATGATGGTAAAAAAAAGAGAGAATCTGTAGGAATCTGGAGGGCCCAGATAGAACAAAAAGACAAACAGTTAGGTTAATGGGGGCAGGAGCGGTCATAATCGGGGCATCTTCTATTCTTAGTTTCAACTGATTTTAGTCGAAAAATGAAATAGAAGCTTTCAGAGATGTTTGATTATAACTTGAACGGATTGGAACAGGGCCTTTCGTTGTCAATTGTCAAAACTTTTAATAGAATTTCCATATAACTGCATAGAATAGTACGATTTTAATCTTCGATTCAAGAGATGAGTACATATAAATTCCAGTTGAAGCTATGCTAATTTGACATATAATTGCCGAGAACTGCAATTTGCCTTATTAGTGATTATAATTATCTCTTGTTACTGTTAACTGATTAGTTCACGATGCTCACAAATCATACCTGTGACTATGAGGTTTTCATATTCTCATTTATTTGTATAGAACTTACTAGAAGATACCATCACATTCTTACCATATACTTTCTATATTTTTTTCCTTTTTAATCCTATCTGTACAAAGATTACCTCCGTGTTATTGCCTGGGATTAGTTGGTGAAGTTAATTTATGTTTTATTTTGAATTTGTCTGGTAAAATTGCGAACCTGCTAATTGGATATATTAGTCGGCCACGGTTGCACAAACTTCACTATTTTCAAACTAGTGGTTAAAGACTAAAGTTATTGACTTTTCAAAACTCAGAAGCTTTTGGGTTATTACAGTGTCGTCATTATGTAAGTGTTTTTTGAAATGATATGACGTTTTATGCTTCAAATTAGAGCTAAAGTTAAATTGTTGATTCTTCATTCTGTTCAAGCCATATTATTATGATTAATTTTTTATGCATTTGGTCTTGAGCAACTTCAATTTGGAAATTATGCTTAATACCTTTTCTTTTGACGTTGTCTTTTGCATTTTATTTCTGAAAAACAGGACCTAAGGAGTTCATTTCTCTGTTTTATGGACTTCAGAGCTGGAGGGTTGGTTGAAGACTTATTATTATTTTAGCCAACATTCTTATTTGGTGTAATTTGTTTAAACTGAATATTTGTTGAATCTTTTTCAGGGTATTTTTGAGAGGTTTGATTCAGACAGAAGTGGAAAAATTGATTCCAATGAGTTGCGAGAAGCACTTTTGAGTTTGGGCTTTGCAGTCTCACCAATGGTCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGTGGTGGGAAAAGCAAGGCCATTGAATACGATAATTTTATCGAGTACGTTTGTCTTCTTTACCCTTGATTTATGGTGCTACTCTTTTTACAATGAGAGGTTTTGAGTTATGCTTTAAATGTTAAGCTTTCACTTGTCATTAATCACTGTTCTTATTCTATTCTTTTCTTTTTGCCAATGCAGGTGTTGTCTTACTGTCAAGGTGAGTTTCTGACTATTTTTGTTTGCATGTCTTTGTATATTAAAACATGAACTGTTTAAAGGCAATGACATCATCTTTGAAGAAATGCTTATTCTGCAATCTGTACTTTTTGTCATGCTCAAAATCACTTCGAAATACACCTTTGGTAACTTAAAATTAGTTAAATATTTAATTTTACACTTGTACAAGCAATTTTCATTTCATCAATATTGGTTTTGAATCATTAAAAGCATGTTTCATTATGATTTTAAATTAAAAACAACAAAAAATTATTTTAAAAGTTTTAGAATCACTCCTAAACATGCATAGTTGGACACATTTTCCTCGTTGTCTTCATTCTGTTCCACTCATGTTGATGCCTATGAGTTTAACTGTGGCTGCCAAGTAGTTACTTAAAATCCAATTTTGCCATGAGATATTGCTGTTGTTATTTTCATTTTTTTCTCCCTTCTATTTTTCACACACTGATAGTTTAAGCTCCTTGTATGACCCTTTAACTCAATTCTCCAGCAACGAAAACGTTTTGATTTTTAAGAATTCCTGAAACATTTTGTTTGCGTTGGGGAGAAAGAGTAGACTAAAACTGAAGTGATTTTGATGTAATATTTTACAGGGATTGACTGAAAAATTCAAGGAAAAGGACACTACATACTCTGGTTCTGCAACCTTCAGTTATGAGGCTTTCATGTTGACTGTCTTACCATTTCTCATTGCCTGAGGAGTAAGCCTTCGTTCGAGCACGTTTGTTCAGTGTAAAGCTTGAGTTTTCTTTGGCTTTTTCTTTTGATTTCCATTATGTTTGATAGAATCTATTAATGTAGCCTCATCCTTATCAATGTTATAAGTTATGTTCTTTGTATAAATTGGCTATCTTAAGTATTCAATGATAGTTCTATGAATATCTCAACTGTTTACCTGTTTCCCCGCCTGGATTTCCAATTTGGCATTTGACAGTAAAAGTTAGGTCGTTGAATCATATATATAATGTATCCTCTTCTGCTTTTTATGATAAATATTGTGGATGAAGTTTGGAGTGTTCAATTGCAAGATATTCTGGGTCGA

mRNA sequence

GAACACGTTATTTCTACTTGGGATCAGCAATTTCAATTTTTTTTTTCCTGAAAATGTCAGGTTACCCAAATCAACCATCAGGTTACCCAAATCAACCATCAGGTTACGGTTACGGCTACGGCGGCACCGGTGGCTACGGAGCTCCACCACCGACTGCTCAGCCTTACAATTCTTCAGCTTACGGACATCCACAGTCCCAGACTCAGCAGCCGTACGCTCCGGTGGCCGCGCCATATGGCGTACCGTCCGCACCGTACGGGTCAGCTCAGGGCGAAAAACCGCCCAAGGACAAGCCTAATCAGTCGTACGGCGGTGCCGGTGGTGGTCATCAGTCTTACGGCGGAGCAGCCAGTGGTGGTCATGGTTATCCACCTCCTTCTGCTTATGGAAGCCCTTTTGCTTCACTCTTGCCATCGGCTTTTCCGCCTGGTACTGATCCGAGCATCGTTGCTTGTTTTCAAGTAGCCGATCAGGATGGTAGTGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTATCATCGTATAATCAGAAGTTTAGCATCAGAACTGTTCATCTTCTTATGTACCATTTCACCAATACGAATACCAGGAAGATCGGACCTAAGGAGTTCATTTCTCTGTTTTATGGACTTCAGAGCTGGAGGGGTATTTTTGAGAGGTTTGATTCAGACAGAAGTGGAAAAATTGATTCCAATGAGTTGCGAGAAGCACTTTTGAGTTTGGGCTTTGCAGTCTCACCAATGGTCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGTGGTGGGAAAAGCAAGGCCATTGAATACGATAATTTTATCGAGTGTTGTCTTACTGTCAAGGGATTGACTGAAAAATTCAAGGAAAAGGACACTACATACTCTGGTTCTGCAACCTTCAGTTATGAGGCTTTCATGTTGACTGTCTTACCATTTCTCATTGCCTGAGGAGTAAGCCTTCGTTCGAGCACGTTTGTTCAGTGTAAAGCTTGAGTTTTCTTTGGCTTTTTCTTTTGATTTCCATTATGTTTGATAGAATCTATTAATGTAGCCTCATCCTTATCAATGTTATAAGTTATGTTCTTTGTATAAATTGGCTATCTTAAGTATTCAATGATAGTTCTATGAATATCTCAACTGTTTACCTGTTTCCCCGCCTGGATTTCCAATTTGGCATTTGACAGTAAAAGTTAGGTCGTTGAATCATATATATAATGTATCCTCTTCTGCTTTTTATGATAAATATTGTGGATGAAGTTTGGAGTGTTCAATTGCAAGATATTCTGGGTCGA

Coding sequence (CDS)

ATGTCAGGTTACCCAAATCAACCATCAGGTTACCCAAATCAACCATCAGGTTACGGTTACGGCTACGGCGGCACCGGTGGCTACGGAGCTCCACCACCGACTGCTCAGCCTTACAATTCTTCAGCTTACGGACATCCACAGTCCCAGACTCAGCAGCCGTACGCTCCGGTGGCCGCGCCATATGGCGTACCGTCCGCACCGTACGGGTCAGCTCAGGGCGAAAAACCGCCCAAGGACAAGCCTAATCAGTCGTACGGCGGTGCCGGTGGTGGTCATCAGTCTTACGGCGGAGCAGCCAGTGGTGGTCATGGTTATCCACCTCCTTCTGCTTATGGAAGCCCTTTTGCTTCACTCTTGCCATCGGCTTTTCCGCCTGGTACTGATCCGAGCATCGTTGCTTGTTTTCAAGTAGCCGATCAGGATGGTAGTGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTATCATCGTATAATCAGAAGTTTAGCATCAGAACTGTTCATCTTCTTATGTACCATTTCACCAATACGAATACCAGGAAGATCGGACCTAAGGAGTTCATTTCTCTGTTTTATGGACTTCAGAGCTGGAGGGGTATTTTTGAGAGGTTTGATTCAGACAGAAGTGGAAAAATTGATTCCAATGAGTTGCGAGAAGCACTTTTGAGTTTGGGCTTTGCAGTCTCACCAATGGTCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGTGGTGGGAAAAGCAAGGCCATTGAATACGATAATTTTATCGAGTGTTGTCTTACTGTCAAGGGATTGACTGAAAAATTCAAGGAAAAGGACACTACATACTCTGGTTCTGCAACCTTCAGTTATGAGGCTTTCATGTTGACTGTCTTACCATTTCTCATTGCCTGA

Protein sequence

MSGYPNQPSGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPYGVPSAPYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA*
Homology
BLAST of CSPI04G17190 vs. ExPASy Swiss-Prot
Match: Q8W4L0 (Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 PE=2 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 2.8e-99
Identity = 212/341 (62.17%), Postives = 236/341 (69.21%), Query Frame = 0

Query: 9   SGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSA-----YGHPQSQTQQPY-----APVA 68
           SGYP  PS  GYGYGG      PPP   PY S+      YG   S    PY     +P A
Sbjct: 2   SGYP--PSSQGYGYGGN-----PPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSSASSPYA 61

Query: 69  APYGV--------PSAPYGSAQGE--KPPKDKPN--------------------QSYGGA 128
            PYG         PSAPY S  G+  KP K+KP+                      YGG 
Sbjct: 62  VPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGY 121

Query: 129 GGGHQ------SYGGAA-----SGGHGYPPPSA-YGSPFASLLPSAFPPGTDPSIVACFQ 188
           GG  Q       YGGA       GG+G PPP A YGSPFASL+PSAFPPGTDP+IVACFQ
Sbjct: 122 GGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQ 181

Query: 189 VADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFISLFYGLQS 248
            AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF SLF+ LQ+
Sbjct: 182 AADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQN 241

Query: 249 WRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFI 298
           WR IFERFD DRSG+ID+NELR+AL+SLGF+VSP++LDLLVSKFDKSGG+++AIEYDNFI
Sbjct: 242 WRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFI 301

BLAST of CSPI04G17190 vs. ExPASy Swiss-Prot
Match: Q9FYE4 (Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 PE=2 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 1.8e-98
Identity = 213/357 (59.66%), Postives = 238/357 (66.67%), Query Frame = 0

Query: 9   SGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPYGV----- 68
           SGYP    GYGYGYGG    G  PP  QP  SS   +P   +    +P A PYG      
Sbjct: 2   SGYPPTSQGYGYGYGG----GNQPPPPQPPYSSGGNNPPYGSSTTSSPYAVPYGASKPQS 61

Query: 69  -----------------PSAPYGSAQGE--KPPKDKP------------NQSYG------ 128
                            PSAPY  + G+  KPPK+KP            +  YG      
Sbjct: 62  SSSSAPTYGSSSYGAPPPSAPYAPSPGDYNKPPKEKPYGGGYGAPPPSGSSDYGSYGAGP 121

Query: 129 ------GAGGG-----------HQSYGGA----ASGGH-----GYPPPSAYGSPFASLLP 188
                 G GGG           + SYGGA    AS GH     GYPP ++YGSPFASL+P
Sbjct: 122 RPSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASYGSPFASLIP 181

Query: 189 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 248
           S F PGTDP+IVACFQ ADQDGSGFIDDKELQG LSSY Q+FS+RTVHLLMY FTN+N  
Sbjct: 182 SGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFTNSNAM 241

Query: 249 KIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKF 298
           KIGPKEF +LFY LQ+WR IFER D DRSG+ID NELR+ALLSLGF+VSP+VLDLLVSKF
Sbjct: 242 KIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPVVLDLLVSKF 301

BLAST of CSPI04G17190 vs. ExPASy Swiss-Prot
Match: Q2QY10 (Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 3.7e-96
Identity = 201/307 (65.47%), Postives = 219/307 (71.34%), Query Frame = 0

Query: 9   SGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPYGV----- 68
           +GYP  P G GY YGG GGYGAPPP   PY SS             AP A PYG      
Sbjct: 2   AGYPPNP-GSGYPYGGAGGYGAPPP---PYGSSP------------APSAPPYGAKPPKE 61

Query: 69  -----PSAPY--GSAQGEKPPKDKPNQSYGGAGGGHQS------YGGAASGGHGYPPPSA 128
                 SAPY  G      PP  +P  S GG G    S      YG        Y  P  
Sbjct: 62  GKTSSSSAPYYGGGGGYGAPPSTQPYGSGGGYGAPPSSQPYGAPYGAPPPSSAPYGAPGG 121

Query: 129 YGSPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLL 188
           YGSPFASL+PSAFPPGTDP++VACFQ AD+DGSG IDDKELQ  LS Y+Q FS+RTVHLL
Sbjct: 122 YGSPFASLVPSAFPPGTDPNVVACFQAADRDGSGMIDDKELQSALSGYSQSFSLRTVHLL 181

Query: 189 MYHFTNTNTRKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSP 248
           MY FTNTN RKIGPKEF S+FY LQ+WR IFERFD DRSGKID+ ELR+ALLSLG++VSP
Sbjct: 182 MYLFTNTNVRKIGPKEFTSVFYSLQNWRSIFERFDRDRSGKIDATELRDALLSLGYSVSP 241

Query: 249 MVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLT 298
            VLDLLVSKFDK+GGK+KAIEYDNFIECCLTVKGLTEKFKEKDT +SGSATF+YEAFMLT
Sbjct: 242 TVLDLLVSKFDKTGGKNKAIEYDNFIECCLTVKGLTEKFKEKDTAFSGSATFTYEAFMLT 292

BLAST of CSPI04G17190 vs. ExPASy Swiss-Prot
Match: Q9ZQH1 (Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 PE=2 SV=2)

HSP 1 Score: 148.7 bits (374), Expect = 1.1e-34
Identity = 86/208 (41.35%), Postives = 128/208 (61.54%), Query Frame = 0

Query: 93  QSYGGAASGGHGYP--PPSAYGSPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKE 152
           Q + G +   + YP   P+   S ++ +    F P T P IV  F+ AD++ SGF+++ E
Sbjct: 21  QQHDGESRYTYAYPSYQPTQQFSSYSGM----FSPETHPEIVRSFESADRNRSGFLEESE 80

Query: 153 LQGVLS-SYNQKFSIRTVHLLM--YHFTNTNTRKIGPKEFISLFYGLQSWRGIFERFDSD 212
           L+  LS S     S RT+ LL+  Y     +  ++GPKE++ L+  L  WR IF R+D D
Sbjct: 81  LRQALSLSGYDGISNRTIRLLLFIYKIPVDSLLRLGPKEYVELWNCLAQWRAIFNRYDRD 140

Query: 213 RSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTE 272
           RSGK++S +LR+A  +LG  +   V  L+VS+FD   GK+  + +D+F+EC + VKGLTE
Sbjct: 141 RSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQFDDGTGKTVDLCFDSFLECGMIVKGLTE 200

Query: 273 KFKEKDTTYSGSATFSYEAFMLTVLPFL 296
           KF+E D  Y+G AT SY+ FML V+PF+
Sbjct: 201 KFRENDPGYTGYATLSYDVFMLMVIPFI 224

BLAST of CSPI04G17190 vs. ExPASy Swiss-Prot
Match: Q9UBV8 (Peflin OS=Homo sapiens OX=9606 GN=PEF1 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 7.9e-30
Identity = 95/284 (33.45%), Postives = 133/284 (46.83%), Query Frame = 0

Query: 10  GYPNQPSGYGYGYGGTGGYGAPP---PTAQPYNSSAYGHPQSQTQQPYAPVAAPYGVPSA 69
           G PN    YG G    GGYG P    P   P     YGHP         P   P G P  
Sbjct: 28  GPPNSGGQYGSGLPPGGGYGGPAPGGPYGPPAGGGPYGHPN--------PGMFPSGTPGG 87

Query: 70  PYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLL-PSAFPP 129
           PYG                          G A  G +G PPPS+YG+    L      PP
Sbjct: 88  PYG--------------------------GAAPGGPYGQPPPSSYGAQQPGLYGQGGAPP 147

Query: 130 GTDPSIVACFQVADQDGSGFIDDKELQGVLSSYN-QKFSIRTVHLLMYHFTNTNTRKIGP 189
             DP   + FQ  D D SG+I  KEL+  L + N   F+  T  +++  F  T + +I  
Sbjct: 148 NVDPEAYSWFQSVDSDHSGYISMKELKQALVNCNWSSFNDETCLMMINMFDKTKSGRIDV 207

Query: 190 KEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSG 249
             F +L+  +Q W+ +F+++D DRSG I   EL++AL  +G+ +SP    LLVS++    
Sbjct: 208 YGFSALWKFIQQWKNLFQQYDRDRSGSISYTELQQALSQMGYNLSPQFTQLLVSRYCPRS 267

Query: 250 GKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFM 289
             + A++ D FI+ C  ++ LTE F+EKDT   G+   S+E F+
Sbjct: 268 A-NPAMQLDRFIQVCTQLQVLTEAFREKDTAVQGNIRLSFEDFV 276

BLAST of CSPI04G17190 vs. ExPASy TrEMBL
Match: A0A0A0L336 (Ef-hand calcium binding protein OS=Cucumis sativus OX=3659 GN=Csa_4G341560 PE=4 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.5e-160
Identity = 288/289 (99.65%), Postives = 288/289 (99.65%), Query Frame = 0

Query: 9   SGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPYGVPSAPY 68
           SGYPNQPSGYGYGYG TGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPYGVPSAPY
Sbjct: 2   SGYPNQPSGYGYGYGSTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPYGVPSAPY 61

Query: 69  GSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLPSAFPPGTD 128
           GSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLPSAFPPGTD
Sbjct: 62  GSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLPSAFPPGTD 121

Query: 129 PSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFI 188
           PSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFI
Sbjct: 122 PSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFI 181

Query: 189 SLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSK 248
           SLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSK
Sbjct: 182 SLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSK 241

Query: 249 AIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           AIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 242 AIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 290

BLAST of CSPI04G17190 vs. ExPASy TrEMBL
Match: A0A5D3BYW0 (Putative calcium-binding protein CML49 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold527G00390 PE=4 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 8.8e-157
Identity = 287/297 (96.63%), Postives = 289/297 (97.31%), Query Frame = 0

Query: 1   MSGYPNQPSGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAP 60
           MSGYPNQPSGY     GYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAP
Sbjct: 1   MSGYPNQPSGY-----GYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAP 60

Query: 61  YGVPSAPYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLP 120
           YG PSAPY SAQGEKPPKDKPNQSYGGAGGGHQSYGGAA GGHGYPPPSAYG+PFASLLP
Sbjct: 61  YGGPSAPY-SAQGEKPPKDKPNQSYGGAGGGHQSYGGAAGGGHGYPPPSAYGNPFASLLP 120

Query: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180
           SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR
Sbjct: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180

Query: 181 KIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKF 240
           KIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELR+ALLSLGFAVSPMVLDLLVSKF
Sbjct: 181 KIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELRDALLSLGFAVSPMVLDLLVSKF 240

Query: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 291

BLAST of CSPI04G17190 vs. ExPASy TrEMBL
Match: A0A1S3CNE3 (probable calcium-binding protein CML49 OS=Cucumis melo OX=3656 GN=LOC103502445 PE=4 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 8.8e-157
Identity = 287/297 (96.63%), Postives = 289/297 (97.31%), Query Frame = 0

Query: 1   MSGYPNQPSGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAP 60
           MSGYPNQPSGY     GYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAP
Sbjct: 1   MSGYPNQPSGY-----GYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAP 60

Query: 61  YGVPSAPYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLP 120
           YG PSAPY SAQGEKPPKDKPNQSYGGAGGGHQSYGGAA GGHGYPPPSAYG+PFASLLP
Sbjct: 61  YGGPSAPY-SAQGEKPPKDKPNQSYGGAGGGHQSYGGAAGGGHGYPPPSAYGNPFASLLP 120

Query: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180
           SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR
Sbjct: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180

Query: 181 KIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKF 240
           KIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELR+ALLSLGFAVSPMVLDLLVSKF
Sbjct: 181 KIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELRDALLSLGFAVSPMVLDLLVSKF 240

Query: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 291

BLAST of CSPI04G17190 vs. ExPASy TrEMBL
Match: A0A5A7VQC6 (Putative calcium-binding protein CML49 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold138G00690 PE=4 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.1e-150
Identity = 281/297 (94.61%), Postives = 283/297 (95.29%), Query Frame = 0

Query: 1   MSGYPNQPSGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAP 60
           MSGYPNQPSGY     GYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAP
Sbjct: 1   MSGYPNQPSGY-----GYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAP 60

Query: 61  YGVPSAPYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLP 120
           YG PSAPY SAQGEKPPKDKPNQSYGGAGGGHQSYGGAA GGHGYPPPSAYG+PFASLLP
Sbjct: 61  YGGPSAPY-SAQGEKPPKDKPNQSYGGAGGGHQSYGGAAGGGHGYPPPSAYGNPFASLLP 120

Query: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180
           SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR
Sbjct: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180

Query: 181 KIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKF 240
           KIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELR+ALLSLGFAVSPMVLDLLVSKF
Sbjct: 181 KIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELRDALLSLGFAVSPMVLDLLVSKF 240

Query: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           DKSGGKSKAIEYDNFIE      GLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIE-----YGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 286

BLAST of CSPI04G17190 vs. ExPASy TrEMBL
Match: A0A6J1HP98 (probable calcium-binding protein CML49 OS=Cucurbita maxima OX=3661 GN=LOC111466073 PE=4 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.0e-144
Identity = 267/291 (91.75%), Postives = 270/291 (92.78%), Query Frame = 0

Query: 9   SGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSAYG--HPQSQTQQPYAPVAAPYGVPSA 68
           SGYPNQPSGYGYGYGG GGYGAPPPTAQPY SS YG  HP   +QQPYAPVAAPYG PSA
Sbjct: 2   SGYPNQPSGYGYGYGGAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAAPYGAPSA 61

Query: 69  PYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLPSAFPPG 128
           PYGS  GEKP KDKPNQS+GG GG HQSY     GGHGYPPPSAYGSPFASLLPSAFPPG
Sbjct: 62  PYGSNPGEKPHKDKPNQSHGGVGGSHQSY-----GGHGYPPPSAYGSPFASLLPSAFPPG 121

Query: 129 TDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKE 188
           TDPSIV CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKE
Sbjct: 122 TDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKE 181

Query: 189 FISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGK 248
           FI+LFYGLQSWRGIFERFDSDRSGKID NELREALLSLGFAVSPMVLDLLVSKFDKSGGK
Sbjct: 182 FIALFYGLQSWRGIFERFDSDRSGKIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGGK 241

Query: 249 SKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           SKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 242 SKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 287

BLAST of CSPI04G17190 vs. NCBI nr
Match: XP_004142653.1 (probable calcium-binding protein CML49 [Cucumis sativus] >KGN54506.1 hypothetical protein Csa_011963 [Cucumis sativus])

HSP 1 Score: 573.2 bits (1476), Expect = 1.3e-159
Identity = 288/289 (99.65%), Postives = 288/289 (99.65%), Query Frame = 0

Query: 9   SGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPYGVPSAPY 68
           SGYPNQPSGYGYGYG TGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPYGVPSAPY
Sbjct: 2   SGYPNQPSGYGYGYGSTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPYGVPSAPY 61

Query: 69  GSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLPSAFPPGTD 128
           GSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLPSAFPPGTD
Sbjct: 62  GSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLPSAFPPGTD 121

Query: 129 PSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFI 188
           PSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFI
Sbjct: 122 PSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFI 181

Query: 189 SLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSK 248
           SLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSK
Sbjct: 182 SLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSK 241

Query: 249 AIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           AIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 242 AIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 290

BLAST of CSPI04G17190 vs. NCBI nr
Match: XP_008464601.1 (PREDICTED: probable calcium-binding protein CML49 [Cucumis melo] >TYK04310.1 putative calcium-binding protein CML49 [Cucumis melo var. makuwa])

HSP 1 Score: 562.8 bits (1449), Expect = 1.8e-156
Identity = 287/297 (96.63%), Postives = 289/297 (97.31%), Query Frame = 0

Query: 1   MSGYPNQPSGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAP 60
           MSGYPNQPSGY     GYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAP
Sbjct: 1   MSGYPNQPSGY-----GYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAP 60

Query: 61  YGVPSAPYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLP 120
           YG PSAPY SAQGEKPPKDKPNQSYGGAGGGHQSYGGAA GGHGYPPPSAYG+PFASLLP
Sbjct: 61  YGGPSAPY-SAQGEKPPKDKPNQSYGGAGGGHQSYGGAAGGGHGYPPPSAYGNPFASLLP 120

Query: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180
           SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR
Sbjct: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180

Query: 181 KIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKF 240
           KIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELR+ALLSLGFAVSPMVLDLLVSKF
Sbjct: 181 KIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELRDALLSLGFAVSPMVLDLLVSKF 240

Query: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 291

BLAST of CSPI04G17190 vs. NCBI nr
Match: XP_038883807.1 (probable calcium-binding protein CML50 [Benincasa hispida])

HSP 1 Score: 550.1 bits (1416), Expect = 1.2e-152
Identity = 281/298 (94.30%), Postives = 285/298 (95.64%), Query Frame = 0

Query: 1   MSGYPNQPSGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQ-SQTQQPYAPVAA 60
           MSGYPNQPSGY     GYGYGYGG+GGYGAPPPTAQ YNSSAYGHPQ  QTQQPYAPVAA
Sbjct: 1   MSGYPNQPSGY-----GYGYGYGGSGGYGAPPPTAQVYNSSAYGHPQPQQTQQPYAPVAA 60

Query: 61  PYGVPSAPYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLL 120
           PYG PSAPYGSA GEKPPKDKPNQSYGGAGGGHQSYGGA SGGHGYPPPSAYG+PFASLL
Sbjct: 61  PYGAPSAPYGSAPGEKPPKDKPNQSYGGAGGGHQSYGGAGSGGHGYPPPSAYGNPFASLL 120

Query: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180
           PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQ VLSSY+QKFSIRTVHLLMYHFTNTNT
Sbjct: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQRVLSSYDQKFSIRTVHLLMYHFTNTNT 180

Query: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSK 240
           RKIGPKEFI+LFYGLQSWRGIFERFDSDRSGKIDS ELREALLSLGFAVSPMVLDLLVSK
Sbjct: 181 RKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSKELREALLSLGFAVSPMVLDLLVSK 240

Query: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 293

BLAST of CSPI04G17190 vs. NCBI nr
Match: KAA0067905.1 (putative calcium-binding protein CML49 [Cucumis melo var. makuwa])

HSP 1 Score: 541.6 bits (1394), Expect = 4.3e-150
Identity = 281/297 (94.61%), Postives = 283/297 (95.29%), Query Frame = 0

Query: 1   MSGYPNQPSGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAP 60
           MSGYPNQPSGY     GYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAP
Sbjct: 1   MSGYPNQPSGY-----GYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAP 60

Query: 61  YGVPSAPYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLP 120
           YG PSAPY SAQGEKPPKDKPNQSYGGAGGGHQSYGGAA GGHGYPPPSAYG+PFASLLP
Sbjct: 61  YGGPSAPY-SAQGEKPPKDKPNQSYGGAGGGHQSYGGAAGGGHGYPPPSAYGNPFASLLP 120

Query: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180
           SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR
Sbjct: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180

Query: 181 KIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKF 240
           KIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELR+ALLSLGFAVSPMVLDLLVSKF
Sbjct: 181 KIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELRDALLSLGFAVSPMVLDLLVSKF 240

Query: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           DKSGGKSKAIEYDNFIE      GLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIE-----YGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 286

BLAST of CSPI04G17190 vs. NCBI nr
Match: XP_022966416.1 (probable calcium-binding protein CML49 [Cucurbita maxima])

HSP 1 Score: 522.7 bits (1345), Expect = 2.1e-144
Identity = 267/291 (91.75%), Postives = 270/291 (92.78%), Query Frame = 0

Query: 9   SGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSAYG--HPQSQTQQPYAPVAAPYGVPSA 68
           SGYPNQPSGYGYGYGG GGYGAPPPTAQPY SS YG  HP   +QQPYAPVAAPYG PSA
Sbjct: 2   SGYPNQPSGYGYGYGGAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAAPYGAPSA 61

Query: 69  PYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLPSAFPPG 128
           PYGS  GEKP KDKPNQS+GG GG HQSY     GGHGYPPPSAYGSPFASLLPSAFPPG
Sbjct: 62  PYGSNPGEKPHKDKPNQSHGGVGGSHQSY-----GGHGYPPPSAYGSPFASLLPSAFPPG 121

Query: 129 TDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKE 188
           TDPSIV CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKE
Sbjct: 122 TDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKE 181

Query: 189 FISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGK 248
           FI+LFYGLQSWRGIFERFDSDRSGKID NELREALLSLGFAVSPMVLDLLVSKFDKSGGK
Sbjct: 182 FIALFYGLQSWRGIFERFDSDRSGKIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGGK 241

Query: 249 SKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           SKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 242 SKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 287

BLAST of CSPI04G17190 vs. TAIR 10
Match: AT3G10300.3 (Calcium-binding EF-hand family protein )

HSP 1 Score: 363.2 bits (931), Expect = 2.0e-100
Identity = 212/341 (62.17%), Postives = 236/341 (69.21%), Query Frame = 0

Query: 9   SGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSA-----YGHPQSQTQQPY-----APVA 68
           SGYP  PS  GYGYGG      PPP   PY S+      YG   S    PY     +P A
Sbjct: 2   SGYP--PSSQGYGYGGN-----PPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSSASSPYA 61

Query: 69  APYGV--------PSAPYGSAQGE--KPPKDKPN--------------------QSYGGA 128
            PYG         PSAPY S  G+  KP K+KP+                      YGG 
Sbjct: 62  VPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGY 121

Query: 129 GGGHQ------SYGGAA-----SGGHGYPPPSA-YGSPFASLLPSAFPPGTDPSIVACFQ 188
           GG  Q       YGGA       GG+G PPP A YGSPFASL+PSAFPPGTDP+IVACFQ
Sbjct: 122 GGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQ 181

Query: 189 VADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFISLFYGLQS 248
            AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF SLF+ LQ+
Sbjct: 182 AADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQN 241

Query: 249 WRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFI 298
           WR IFERFD DRSG+ID+NELR+AL+SLGF+VSP++LDLLVSKFDKSGG+++AIEYDNFI
Sbjct: 242 WRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFI 301

BLAST of CSPI04G17190 vs. TAIR 10
Match: AT5G04170.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 360.5 bits (924), Expect = 1.3e-99
Identity = 213/357 (59.66%), Postives = 238/357 (66.67%), Query Frame = 0

Query: 9   SGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPYGV----- 68
           SGYP    GYGYGYGG    G  PP  QP  SS   +P   +    +P A PYG      
Sbjct: 2   SGYPPTSQGYGYGYGG----GNQPPPPQPPYSSGGNNPPYGSSTTSSPYAVPYGASKPQS 61

Query: 69  -----------------PSAPYGSAQGE--KPPKDKP------------NQSYG------ 128
                            PSAPY  + G+  KPPK+KP            +  YG      
Sbjct: 62  SSSSAPTYGSSSYGAPPPSAPYAPSPGDYNKPPKEKPYGGGYGAPPPSGSSDYGSYGAGP 121

Query: 129 ------GAGGG-----------HQSYGGA----ASGGH-----GYPPPSAYGSPFASLLP 188
                 G GGG           + SYGGA    AS GH     GYPP ++YGSPFASL+P
Sbjct: 122 RPSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASYGSPFASLIP 181

Query: 189 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 248
           S F PGTDP+IVACFQ ADQDGSGFIDDKELQG LSSY Q+FS+RTVHLLMY FTN+N  
Sbjct: 182 SGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFTNSNAM 241

Query: 249 KIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKF 298
           KIGPKEF +LFY LQ+WR IFER D DRSG+ID NELR+ALLSLGF+VSP+VLDLLVSKF
Sbjct: 242 KIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPVVLDLLVSKF 301

BLAST of CSPI04G17190 vs. TAIR 10
Match: AT3G10300.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 295.0 bits (754), Expect = 6.6e-80
Identity = 178/301 (59.14%), Postives = 200/301 (66.45%), Query Frame = 0

Query: 9   SGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSA-----YGHPQSQTQQPY-----APVA 68
           SGYP  PS  GYGYGG      PPP   PY S+      YG   S    PY     +P A
Sbjct: 2   SGYP--PSSQGYGYGGN-----PPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSSASSPYA 61

Query: 69  APYGV--------PSAPYGSAQGE--KPPKDKPN--------------------QSYGGA 128
            PYG         PSAPY S  G+  KP K+KP+                      YGG 
Sbjct: 62  VPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGY 121

Query: 129 GGGHQ------SYGGAA-----SGGHGYPPPSA-YGSPFASLLPSAFPPGTDPSIVACFQ 188
           GG  Q       YGGA       GG+G PPP A YGSPFASL+PSAFPPGTDP+IVACFQ
Sbjct: 122 GGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQ 181

Query: 189 VADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFISLFYGLQS 248
            AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF SLF+ LQ+
Sbjct: 182 AADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQN 241

Query: 249 WRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFI 258
           WR IFERFD DRSG+ID+NELR+AL+SLGF+VSP++LDLLVSKFDKSGG+++AIEYDNFI
Sbjct: 242 WRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFI 295

BLAST of CSPI04G17190 vs. TAIR 10
Match: AT3G10300.4 (Calcium-binding EF-hand family protein )

HSP 1 Score: 295.0 bits (754), Expect = 6.6e-80
Identity = 178/301 (59.14%), Postives = 200/301 (66.45%), Query Frame = 0

Query: 9   SGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSA-----YGHPQSQTQQPY-----APVA 68
           SGYP  PS  GYGYGG      PPP   PY S+      YG   S    PY     +P A
Sbjct: 2   SGYP--PSSQGYGYGGN-----PPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSSASSPYA 61

Query: 69  APYGV--------PSAPYGSAQGE--KPPKDKPN--------------------QSYGGA 128
            PYG         PSAPY S  G+  KP K+KP+                      YGG 
Sbjct: 62  VPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGY 121

Query: 129 GGGHQ------SYGGAA-----SGGHGYPPPSA-YGSPFASLLPSAFPPGTDPSIVACFQ 188
           GG  Q       YGGA       GG+G PPP A YGSPFASL+PSAFPPGTDP+IVACFQ
Sbjct: 122 GGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQ 181

Query: 189 VADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFISLFYGLQS 248
            AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF SLF+ LQ+
Sbjct: 182 AADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQN 241

Query: 249 WRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFI 258
           WR IFERFD DRSG+ID+NELR+AL+SLGF+VSP++LDLLVSKFDKSGG+++AIEYDNFI
Sbjct: 242 WRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFI 295

BLAST of CSPI04G17190 vs. TAIR 10
Match: AT3G10300.5 (Calcium-binding EF-hand family protein )

HSP 1 Score: 290.4 bits (742), Expect = 1.6e-78
Identity = 178/302 (58.94%), Postives = 200/302 (66.23%), Query Frame = 0

Query: 9   SGYPNQPSGYGYGYGGTGGYGAPPPTAQPYNSSA-----YGHPQSQTQQPY-----APVA 68
           SGYP  PS  GYGYGG      PPP   PY S+      YG   S    PY     +P A
Sbjct: 2   SGYP--PSSQGYGYGGN-----PPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSSASSPYA 61

Query: 69  APYGV--------PSAPYGSAQGE--KPPKDKPN--------------------QSYGGA 128
            PYG         PSAPY S  G+  KP K+KP+                      YGG 
Sbjct: 62  VPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGY 121

Query: 129 GGGHQ------SYGGAA-----SGGHGYPPPSA-YGSPFASLLPSAFPPGTDPSIVACFQ 188
           GG  Q       YGGA       GG+G PPP A YGSPFASL+PSAFPPGTDP+IVACFQ
Sbjct: 122 GGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQ 181

Query: 189 VADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIG-PKEFISLFYGLQ 248
            AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIG PKEF SLF+ LQ
Sbjct: 182 AADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGRPKEFTSLFFSLQ 241

Query: 249 SWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNF 258
           +WR IFERFD DRSG+ID+NELR+AL+SLGF+VSP++LDLLVSKFDKSGG+++AIEYDNF
Sbjct: 242 NWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNF 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8W4L02.8e-9962.17Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 ... [more]
Q9FYE41.8e-9859.66Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 ... [more]
Q2QY103.7e-9665.47Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2... [more]
Q9ZQH11.1e-3441.35Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 ... [more]
Q9UBV87.9e-3033.45Peflin OS=Homo sapiens OX=9606 GN=PEF1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L3366.5e-16099.65Ef-hand calcium binding protein OS=Cucumis sativus OX=3659 GN=Csa_4G341560 PE=4 ... [more]
A0A5D3BYW08.8e-15796.63Putative calcium-binding protein CML49 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CNE38.8e-15796.63probable calcium-binding protein CML49 OS=Cucumis melo OX=3656 GN=LOC103502445 P... [more]
A0A5A7VQC62.1e-15094.61Putative calcium-binding protein CML49 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1HP981.0e-14491.75probable calcium-binding protein CML49 OS=Cucurbita maxima OX=3661 GN=LOC1114660... [more]
Match NameE-valueIdentityDescription
XP_004142653.11.3e-15999.65probable calcium-binding protein CML49 [Cucumis sativus] >KGN54506.1 hypothetica... [more]
XP_008464601.11.8e-15696.63PREDICTED: probable calcium-binding protein CML49 [Cucumis melo] >TYK04310.1 put... [more]
XP_038883807.11.2e-15294.30probable calcium-binding protein CML50 [Benincasa hispida][more]
KAA0067905.14.3e-15094.61putative calcium-binding protein CML49 [Cucumis melo var. makuwa][more]
XP_022966416.12.1e-14491.75probable calcium-binding protein CML49 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G10300.32.0e-10062.17Calcium-binding EF-hand family protein [more]
AT5G04170.11.3e-9959.66Calcium-binding EF-hand family protein [more]
AT3G10300.26.6e-8059.14Calcium-binding EF-hand family protein [more]
AT3G10300.46.6e-8059.14Calcium-binding EF-hand family protein [more]
AT3G10300.51.6e-7858.94Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 196..224
e-value: 0.0097
score: 25.1
coord: 130..158
e-value: 0.092
score: 21.9
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 131..154
e-value: 5.1E-5
score: 22.6
IPR002048EF-hand domainPFAMPF13405EF-hand_6coord: 197..225
e-value: 1.9E-5
score: 24.1
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 192..227
score: 12.867577
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 126..161
score: 10.691735
NoneNo IPR availableGENE3D1.10.238.10coord: 114..263
e-value: 2.0E-32
score: 114.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 34..55
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..102
NoneNo IPR availablePANTHERPTHR46824:SF1CALCIUM-BINDING PROTEIN CML49-RELATEDcoord: 9..261
IPR044590Probable calcium-binding protein CML48/49/50PANTHERPTHR46824CALCIUM-BINDING PROTEIN CML48-RELATEDcoord: 9..261
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 205..217
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 139..151
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 125..260

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G17190.1CSPI04G17190.1mRNA
CSPI04G17190.2CSPI04G17190.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding