CSPI04G17180 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G17180
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionubiquitin-conjugating enzyme E2 22
LocationChr4: 14611183 .. 14615909 (-)
RNA-Seq ExpressionCSPI04G17180
SyntenyCSPI04G17180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCATTTCAAGGAAAAGAGAAGCTTTTTTCTTTTCTCTCAATACTCTCATCGTCTCTGTTTCCTCCCATTCTTCTTCTTTCTTAGTTTGGTTCCTGAGAATAATCAGAGCCACCGAGAGTTTTCAAAGGTAGTCTTTTCCTCATCTGGGTTTTCATTTTTCTCACTTTAACGCCAACCCCTTTTGATTCGTTTCTGACTTTTGTTTTTCTCACTCTAATCATGTTTCTGCTCACAATCTCATTTCGATAATCTTCTTTTCCATCTCTTTACTTACTAATTGCTTGATTCCTTTCCGATTAGGATAAATGGGGCTTCTTGTTCTTGTTACTTTATTTTGAATGTGTAAATGGGGATGAGGGATTTTGGAGAATTAGGGTTTTTCTTGATTGTTCTTACCCTCGATTGGAACATTTGTTTCTATCAGGGTTTGTTCTTCCGATTCTGAAGTTTGCGAATTTGGAGTTAGTTTTAGCAATCACTGCACTTCTTTTTCAGTATTTTTATTAATTTAAAAGCCGCGGCATCTGATTCGTGTATCGCTTCCATGAATTGCGGCTCCGAGACACTGCTTTACGGGAATAAAGAGTTCTAACAATGCTTTAGTTTCCCTGTGAAACTTCACTTTTGGGGTTTTAGTAACCCGAAGTGTTTTCTGAAATTAAATGCTTTTTGTTTCTGATGTTTGATTTTGGAGCTCTATTCGTATATTCTTGCTTCGTGTCTTGTTAACTTTGAATGCTTTGAAAAGTATGCTCAAATCAGTGGTGATTGCTTGCTATTGATTTCAAGGTTTGTTGCCTGTGTTTATTTTTTAGTATGAGTAACGCTTTTCCTTTTTGTTCTTCATCTTGGTGACGTGACCTTTGTAGGATTATGCTTAACACCTAGACCATAGAAGCTTAAAGACCTGGGAATGTGGAAGCTGTTAATAATTTGACTTTTTTATTTAAGAGTTCTGATGTTGAAATCAACAGTTATTTGGTATAGTGTCTGGCAACTATGCTGCTCAATGTCTGTGGGTCCATCAGAAAATGGTATTGTCTAGGATTGAATCCTTAACTGAATGCCCATCTTAGTTGTGACCTCTCCTCTCGTCTACTGAAATTGTTACAACCTAAACCTGCAGTTATGCAATGAGTGGCAACATATGCTGTTTGGTATGTGTGGCAAATGTACCAGTACCAAGTTACTAACTATTGTTGTTTTATGTTTCTGCTTGGGATGTGTTAGAATACCTTTGTACAAAGGTGGTATAGGATCTTAAATTTGGACAACCTACATGCATATTACTCTTGTCATGTTTGCTGGGTGGATCCCTTCTGAATTTGTTCAATCAATTGTGTCAATTCTTCAATGTCCTTGATTTGCTGCATCCCAAAAGCATAGGTGTCCTGGGGAAGATTTAGGTCACAAGTCATGTACATTTCTCTTGAAATTGGGCAGAAGCTTGACTAAGGCTGCAATGAGCATTCTAAAGATTTTACTCTGGCACCTATTTAGTACTGCACCTTTAAGTTTTATTAAAGGTTTTGATGTTTCATGACCCTAGAGATATTTGTTGTTCCTTTGCAAAGCAACATGAATTGAGTACAGATGCCCTGTTCTTATGTTCTGAATGTATCTCAACTTACATCAATAATATACTAAAGTCTGACTTCATTTTTCAATTTGTATGTTAAACTTCCAATTCAATGTGCAGGTTCTTCTTTGCCTCTGGATTGCTTACACTCACCAAGTGGAAGTTTTTCAGTCCAGTTTCTGTTGATTGTTGAAAAAAGTTATTCAATTTTGCAGTTGTGTAGAAGTGCTCTGTTTTCCCCCACTGGATATGGTAATTCTTGGCCTATTGCACATCACATCATATACTTTAATGACCCACCCTGGAGACTGGAGAGGTATACTTGTGTACAGAGGGATGTGGTTATGCTATCAGTTTGAAGGTGTACTTGTGAACAATTTTTAGTTAGATTTCCTGAGCATGGAAGTCTGTTGGCGAGGGAGAGAATAAAGACGATACAGGAGAGAGGGCAAAGGACTCAGGCTTGTAGTTGACAGACCATGCCTCCATTCCAAAAGATTTAGAACTCTGTATATAACTTTTTGAATGCATTACGTTCCCTTTAACTTCGGCTTCAGGAGTGACTGGGTGCAAAAGAAACAGTTAGGAAGAATATATTTTCACACCTTTATCTCCTGGTTTGCTTGCTCGATTTTATGAATGGTTTTGATGTAGAATATGTTTAATTAAGATACAAACAGCTAATGATGCTTTGATGCTCGGATGAAAAACAATTTCTCATTCTGTCTACTGCTGTACCCCATTAATGGTGCATACCTTTCAATTTGATTCAGGCCACTAATGAAAATCTTCCACCAAATGTTATTAAGCAACTTGCAAAGGAGTTGAAGAGCCTTGATGAATCCCCCCCTGAAGGCATAAAAGTAGTTGTTAACGATGATGACTTCTCAACAATATTTGCTGATATTGAAGGCCCAGGTATGTCATTTATCCCTTTTTTAAATGAAGTTCATCTCGTTGACATGGAGCTCACTTTTCTTACTGCATTGTGATTGTTTCACTTGCAGTGGGGACTCCCTACGAAAATGGTCTCTTCCGCATGAAGTTGATATTGTCCCGTGATTTCCCATGTTCTCCTCCCAAAGGTTTGCTCTGATAATGATTTGGTATAATTTGTGAATTAACAGCTTTAGCCTATAGCTGAATTCAATTTGTGGACCATATTTATCTGAGGATCTAATCTGTATGATTAGACGATAAATTGAATTTATACTTCTCCTAGATCCTATTACCTCATCTTTTATTAATTTCTTTATAGGTTACTTCCTTACAAAGATCTTTCATCCGAACATTGCAACCAATGGCGAGATTTGCGTCAACACTTTAAAGAAGGATTGGAATCCAAGCCTCGGACTGCGACATGTTTTAACTGTAATTACTTTTTCAAATTTGTGGCCACTATCATGTTTCTTGCCTGATATCCTCATTGACTGAAGAGAACTGTTTTTACAAATGCTTTAGTACATTGAATTCAGTTTAATATGTTTTGTTCGTTTCAGAATCTAAATAGGTGTTATGTTGAACAGGTTGTTAGGTGTCTACTGATTGAACCATTTCCAGAATCAGCTTTGAATGAACAAGCTGGCAAGATGCTGCTTGAAAATTATGATGAGTATGCCAGACATGCCAGGTTAGTCAGGCCCCTCTCCAAATTATCTGTTTTATCAATATAAGGTGATAAGAAACGCTGGAATGGTTATTATTGTTTATTTAGATAGAATTCAGTTGATTTTATTGGTGCACTCATTTGATGTTTTGGAAACGATTTTATTGTTCGACTCTATTTTTGAAATTTATGTTGGTTTCCACCCAATTTTGTTATTCTTAGTATGGTTTTTAATTTGAACGCGAAGCTTACTTTATTTTTCAACACCCATGTGATGTTGGACCCAATAGTAACTATTCAAACTGAGATTGAACTTGCTCGACTATTGAAACGGCTAAAAGTCCTTTCAAAGTATTCGACTTCTAAAGCATTATGTGAAAAATTTTAGAATCCCTGGAAACCGTGCAAGAGCACTTAAGAAGAGGAGGCTTCTGTTGGAAGTGATTTTATTGTTTCTCTATTTTTGAAATTTATGTTGGTTTCCACCCAATTTTCTTATTCTCAGTATGGTTTTTAATTTGAACGCTAAGGTTAATTTATTTTCAAAAACCAAAATTGAAATAACTATTGAACCAAATCTGTCTGCTTTCTCACATCCACTCCTTTGTTCTATATTTGTTATTAAACATTTAATTTTCTAATAAATTGTAACGTCATCCTTTGAAAGATAAAAACGATGGTATGATCAAAATAACAAGCAGCCAACTTTCGTTTTGCTTTCCCAGGCTCTATACAGGAATCCATGCAAAGCCAAAGCCAAAATTCAAGACTGGAGCCATCTCTGAGTCAACAACCGCCCTCAATGTTGACCCAACGAATGCATCAGCACTTAGTACAGATCTGAAAAACGCATCTGCCACGGCATTGCCTTTGGCATGTCCAACTGCCAATTCTATTACCGTAGTGAGAAGTGGGCAGGAGCAGCAGCAGCCAAATGTTGTTACAGCACTAGCCTCGGAATCTGGAGTCGGAGGCTCCGGTGTGGGTGAAGCACCAGCAGCAATGGTGAAGAAGGAAAGTGGACACTTAAAAATGCAGCAGGTTGGGAAGAAGAAGATAGATGCAAGAAAGAAAAGCTTGAAGAGATTGTGATGTTTTGTTATTTTGAATTGAATTAAAAAATGGATGGTCAATTAATGGCGCAGGGCTTTATTAAGGTTAGCTCTCTTGTTCGATAAAAAGAAGGGAGCTTAGAAGAAAAAAAAATTAAGAATTTAGAATGGAGAGTTAAAACAGAAGTGTTATGCTGTGCAACCAACATGTAAGGAACACACTTTCATCAGCTGAATCACTCATGGAGTCGACTTCTTTCTTCAACATTTCCTTTTCCAGTTTTTAAAAATGTTGCATAAATTTCGCTTAGGTTTCCCTTAAATTACTATGGTCTTGAAAATACAATTTGATCATGGTCTTAATAACAATTTGTTTAATTGTTTTCTTAGGCTTTGGAATTGAAATGTATTGAAGAATACTAAAAATCTAAAGTGCATCAAGATTTAATCCATTTTAAATAAAATAGGTAAGGTTCAAAATTCTCACAC

mRNA sequence

ATCATTTCAAGGAAAAGAGAAGCTTTTTTCTTTTCTCTCAATACTCTCATCGTCTCTGTTTCCTCCCATTCTTCTTCTTTCTTAGTTTGGTTCCTGAGAATAATCAGAGCCACCGAGAGTTTTCAAAGGTTCTTCTTTGCCTCTGGATTGCTTACACTCACCAAGTGGAAGTTTTTCAGTCCAGTTTCTGTTGATTGTTGAAAAAAGTTATTCAATTTTGCAGTTGTGTAGAAGTGCTCTGTTTTCCCCCACTGGATATGGCCACTAATGAAAATCTTCCACCAAATGTTATTAAGCAACTTGCAAAGGAGTTGAAGAGCCTTGATGAATCCCCCCCTGAAGGCATAAAAGTAGTTGTTAACGATGATGACTTCTCAACAATATTTGCTGATATTGAAGGCCCAGTGGGGACTCCCTACGAAAATGGTCTCTTCCGCATGAAGTTGATATTGTCCCGTGATTTCCCATGTTCTCCTCCCAAAGGTTACTTCCTTACAAAGATCTTTCATCCGAACATTGCAACCAATGGCGAGATTTGCGTCAACACTTTAAAGAAGGATTGGAATCCAAGCCTCGGACTGCGACATGTTTTAACTGTTGTTAGGTGTCTACTGATTGAACCATTTCCAGAATCAGCTTTGAATGAACAAGCTGGCAAGATGCTGCTTGAAAATTATGATGAGTATGCCAGACATGCCAGGCTCTATACAGGAATCCATGCAAAGCCAAAGCCAAAATTCAAGACTGGAGCCATCTCTGAGTCAACAACCGCCCTCAATGTTGACCCAACGAATGCATCAGCACTTAGTACAGATCTGAAAAACGCATCTGCCACGGCATTGCCTTTGGCATGTCCAACTGCCAATTCTATTACCGTAGTGAGAAGTGGGCAGGAGCAGCAGCAGCCAAATGTTGTTACAGCACTAGCCTCGGAATCTGGAGTCGGAGGCTCCGGTGTGGGTGAAGCACCAGCAGCAATGGTGAAGAAGGAAAGTGGACACTTAAAAATGCAGCAGGTTGGGAAGAAGAAGATAGATGCAAGAAAGAAAAGCTTGAAGAGATTGTGATGTTTTGTTATTTTGAATTGAATTAAAAAATGGATGGTCAATTAATGGCGCAGGGCTTTATTAAGGTTAGCTCTCTTGTTCGATAAAAAGAAGGGAGCTTAGAAGAAAAAAAAATTAAGAATTTAGAATGGAGAGTTAAAACAGAAGTGTTATGCTGTGCAACCAACATGTAAGGAACACACTTTCATCAGCTGAATCACTCATGGAGTCGACTTCTTTCTTCAACATTTCCTTTTCCAGTTTTTAAAAATGTTGCATAAATTTCGCTTAGGTTTCCCTTAAATTACTATGGTCTTGAAAATACAATTTGATCATGGTCTTAATAACAATTTGTTTAATTGTTTTCTTAGGCTTTGGAATTGAAATGTATTGAAGAATACTAAAAATCTAAAGTGCATCAAGATTTAATCCATTTTAAATAAAATAGGTAAGGTTCAAAATTCTCACAC

Coding sequence (CDS)

ATGGCCACTAATGAAAATCTTCCACCAAATGTTATTAAGCAACTTGCAAAGGAGTTGAAGAGCCTTGATGAATCCCCCCCTGAAGGCATAAAAGTAGTTGTTAACGATGATGACTTCTCAACAATATTTGCTGATATTGAAGGCCCAGTGGGGACTCCCTACGAAAATGGTCTCTTCCGCATGAAGTTGATATTGTCCCGTGATTTCCCATGTTCTCCTCCCAAAGGTTACTTCCTTACAAAGATCTTTCATCCGAACATTGCAACCAATGGCGAGATTTGCGTCAACACTTTAAAGAAGGATTGGAATCCAAGCCTCGGACTGCGACATGTTTTAACTGTTGTTAGGTGTCTACTGATTGAACCATTTCCAGAATCAGCTTTGAATGAACAAGCTGGCAAGATGCTGCTTGAAAATTATGATGAGTATGCCAGACATGCCAGGCTCTATACAGGAATCCATGCAAAGCCAAAGCCAAAATTCAAGACTGGAGCCATCTCTGAGTCAACAACCGCCCTCAATGTTGACCCAACGAATGCATCAGCACTTAGTACAGATCTGAAAAACGCATCTGCCACGGCATTGCCTTTGGCATGTCCAACTGCCAATTCTATTACCGTAGTGAGAAGTGGGCAGGAGCAGCAGCAGCCAAATGTTGTTACAGCACTAGCCTCGGAATCTGGAGTCGGAGGCTCCGGTGTGGGTGAAGCACCAGCAGCAATGGTGAAGAAGGAAAGTGGACACTTAAAAATGCAGCAGGTTGGGAAGAAGAAGATAGATGCAAGAAAGAAAAGCTTGAAGAGATTGTGA

Protein sequence

MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFRMKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLIEPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNASALSTDLKNASATALPLACPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAAMVKKESGHLKMQQVGKKKIDARKKSLKRL*
Homology
BLAST of CSPI04G17180 vs. ExPASy Swiss-Prot
Match: Q9FF66 (Ubiquitin-conjugating enzyme E2 22 OS=Arabidopsis thaliana OX=3702 GN=UBC22 PE=1 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 1.9e-99
Identity = 197/269 (73.23%), Postives = 213/269 (79.18%), Query Frame = 0

Query: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 60
           MA+NENLPPNVIKQLAKELKSLDESPP+GIKVVVND+DFS I ADIEGPVGTPYENGLFR
Sbjct: 1   MASNENLPPNVIKQLAKELKSLDESPPDGIKVVVNDEDFSQICADIEGPVGTPYENGLFR 60

Query: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLI 120
           MKL LS DFP SPPKGYF+TKIFHPN+A+NGEICVNTLKKDWNPSLGLRHVL+VVRCLLI
Sbjct: 61  MKLALSHDFPHSPPKGYFMTKIFHPNVASNGEICVNTLKKDWNPSLGLRHVLSVVRCLLI 120

Query: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 180
           EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNV  TN 
Sbjct: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVGQTNN 180

Query: 181 SALSTDLKNASATALPLACPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAA 240
                     +ATA+P +      +T   S Q+QQ    V   AS S V           
Sbjct: 181 ET------PGAATAIPSSMTDIKRVTT--SAQDQQHVANVVVAASASVV---------TT 240

Query: 241 MVKKESGHLKMQQVGKKKIDARKKSLKRL 270
             K+E+G  K+ Q  KKK+DARKKSLKRL
Sbjct: 241 TQKREAGLAKV-QADKKKVDARKKSLKRL 251

BLAST of CSPI04G17180 vs. ExPASy Swiss-Prot
Match: Q28F89 (Ubiquitin-conjugating enzyme E2 S OS=Xenopus tropicalis OX=8364 GN=ube2s PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.1e-58
Identity = 99/152 (65.13%), Postives = 125/152 (82.24%), Query Frame = 0

Query: 5   ENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFRMKLI 64
           ENLPP++I+Q+ KE+ +L   PPEGIK++ N++D + +  +IEGP GTPY  G+FRMKLI
Sbjct: 6   ENLPPHIIRQVYKEVSTLTSDPPEGIKIIPNEEDITDVQVNIEGPEGTPYAGGIFRMKLI 65

Query: 65  LSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLIEPFP 124
           L +DFP +PPKGYFLTKIFHPN++TNGEICVN LKKDW   LG+RHVL  ++CLLI P P
Sbjct: 66  LGKDFPAAPPKGYFLTKIFHPNVSTNGEICVNVLKKDWKAELGIRHVLLTIKCLLIHPNP 125

Query: 125 ESALNEQAGKMLLENYDEYARHARLYTGIHAK 157
           ESALNE+AG++LLENY+EYA  ARL T IHA+
Sbjct: 126 ESALNEEAGRLLLENYEEYASRARLMTEIHAQ 157

BLAST of CSPI04G17180 vs. ExPASy Swiss-Prot
Match: C1C3R6 (Ubiquitin-conjugating enzyme E2 S OS=Lithobates catesbeianus OX=8400 GN=ube2s PE=2 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 8.1e-58
Identity = 99/151 (65.56%), Postives = 123/151 (81.46%), Query Frame = 0

Query: 5   ENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFRMKLI 64
           ENLPP+VI+Q+ KE+ +L   PPEGIK++ N++D + +   IEGP GTPY  G+FRMKLI
Sbjct: 6   ENLPPHVIRQVYKEVSTLTPDPPEGIKIIPNEEDITDVQVSIEGPEGTPYAGGIFRMKLI 65

Query: 65  LSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLIEPFP 124
           L +DFP +PPKGYFLTKIFHPN+++NGEICVN LKKDW   LG+RHVL  ++CLLI P P
Sbjct: 66  LGKDFPAAPPKGYFLTKIFHPNVSSNGEICVNVLKKDWKAELGIRHVLLTIKCLLIHPNP 125

Query: 125 ESALNEQAGKMLLENYDEYARHARLYTGIHA 156
           ESALNE+AG++LLENY+EYA  ARL T IHA
Sbjct: 126 ESALNEEAGRLLLENYEEYASRARLMTEIHA 156

BLAST of CSPI04G17180 vs. ExPASy Swiss-Prot
Match: Q8AVU2 (Ubiquitin-conjugating enzyme E2 S-B OS=Xenopus laevis OX=8355 GN=ube2s-b PE=2 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.8e-57
Identity = 97/152 (63.82%), Postives = 124/152 (81.58%), Query Frame = 0

Query: 5   ENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFRMKLI 64
           ENLPP++I+++ KE+ +L   PPEGIK++ N++D + +  +IEGP GTPY  G+FRMKLI
Sbjct: 6   ENLPPHIIRRVYKEVSTLTSDPPEGIKIIPNEEDITDVQVNIEGPEGTPYAGGMFRMKLI 65

Query: 65  LSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLIEPFP 124
           L +DFP +PPKGYFLTKIFHPN++ NGEICVN LKKDW   LG+RHVL  ++CLLI P P
Sbjct: 66  LGKDFPAAPPKGYFLTKIFHPNVSNNGEICVNVLKKDWKAELGIRHVLLTIKCLLIHPNP 125

Query: 125 ESALNEQAGKMLLENYDEYARHARLYTGIHAK 157
           ESALNE+AG++LLENY+EYA  ARL T IHA+
Sbjct: 126 ESALNEEAGRLLLENYEEYASRARLMTDIHAQ 157

BLAST of CSPI04G17180 vs. ExPASy Swiss-Prot
Match: A7SE05 (Ubiquitin-conjugating enzyme E2 S OS=Nematostella vectensis OX=45351 GN=v1g237158 PE=3 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.8e-57
Identity = 98/152 (64.47%), Postives = 123/152 (80.92%), Query Frame = 0

Query: 5   ENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFRMKLI 64
           EN  P +IKQ+A+E+  L   PPEGIKV  ND+D + I A IEGP GTPYE G+F++KL+
Sbjct: 9   ENFSPQIIKQVAREIHGLTNDPPEGIKVFSNDEDITDIQASIEGPTGTPYEGGIFKIKLV 68

Query: 65  LSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLIEPFP 124
           L +DFP +PPKG+FLTKIFHPN+A NGEICVNTLKKDW P LG++ VL  V+CLLI P P
Sbjct: 69  LGKDFPAAPPKGFFLTKIFHPNVAKNGEICVNTLKKDWKPDLGIKQVLLTVKCLLIVPNP 128

Query: 125 ESALNEQAGKMLLENYDEYARHARLYTGIHAK 157
           ESALNE+AGK+LLE YD+Y++ A+++T IHAK
Sbjct: 129 ESALNEEAGKLLLERYDDYSKRAKMFTEIHAK 160

BLAST of CSPI04G17180 vs. ExPASy TrEMBL
Match: A0A0A0L0F5 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G340550 PE=3 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 3.7e-146
Identity = 268/269 (99.63%), Postives = 268/269 (99.63%), Query Frame = 0

Query: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 60
           MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR
Sbjct: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 60

Query: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLI 120
           MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLI
Sbjct: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLI 120

Query: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 180
           EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA
Sbjct: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 180

Query: 181 SALSTDLKNASATALPLACPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAA 240
           SALSTDLKNASATALPL CPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAA
Sbjct: 181 SALSTDLKNASATALPLVCPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAA 240

Query: 241 MVKKESGHLKMQQVGKKKIDARKKSLKRL 270
           MVKKESGHLKMQQVGKKKIDARKKSLKRL
Sbjct: 241 MVKKESGHLKMQQVGKKKIDARKKSLKRL 269

BLAST of CSPI04G17180 vs. ExPASy TrEMBL
Match: A0A1S3CLW5 (ubiquitin-conjugating enzyme E2 22 OS=Cucumis melo OX=3656 GN=LOC103502453 PE=3 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.6e-141
Identity = 262/269 (97.40%), Postives = 262/269 (97.40%), Query Frame = 0

Query: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 60
           MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR
Sbjct: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 60

Query: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLI 120
           MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVL VVRCLLI
Sbjct: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRCLLI 120

Query: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 180
           EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA
Sbjct: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 180

Query: 181 SALSTDLKNASATALPLACPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAA 240
           SALSTDLKNASATALPLACPTANS T VRSGQEQQQPNVVTALASESGVG SGVG APAA
Sbjct: 181 SALSTDLKNASATALPLACPTANSTTAVRSGQEQQQPNVVTALASESGVGVSGVGVAPAA 240

Query: 241 MVKKESGHLKMQQVGKKKIDARKKSLKRL 270
            VKKESGHLKMQQV KKKIDARKKSLKRL
Sbjct: 241 TVKKESGHLKMQQVDKKKIDARKKSLKRL 269

BLAST of CSPI04G17180 vs. ExPASy TrEMBL
Match: A0A5A7VIJ3 (Ubiquitin-conjugating enzyme E2 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold138G00920 PE=3 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 4.7e-141
Identity = 261/269 (97.03%), Postives = 262/269 (97.40%), Query Frame = 0

Query: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 60
           +ATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR
Sbjct: 31  VATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 90

Query: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLI 120
           MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVL VVRCLLI
Sbjct: 91  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRCLLI 150

Query: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 180
           EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA
Sbjct: 151 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 210

Query: 181 SALSTDLKNASATALPLACPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAA 240
           SALSTDLKNASATALPLACPTANS T VRSGQEQQQPNVVTALASESGVG SGVG APAA
Sbjct: 211 SALSTDLKNASATALPLACPTANSTTAVRSGQEQQQPNVVTALASESGVGVSGVGVAPAA 270

Query: 241 MVKKESGHLKMQQVGKKKIDARKKSLKRL 270
            VKKESGHLKMQQV KKKIDARKKSLKRL
Sbjct: 271 TVKKESGHLKMQQVDKKKIDARKKSLKRL 299

BLAST of CSPI04G17180 vs. ExPASy TrEMBL
Match: A0A5D3BZH9 (Ubiquitin-conjugating enzyme E2 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold527G00180 PE=3 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 1.8e-140
Identity = 260/269 (96.65%), Postives = 261/269 (97.03%), Query Frame = 0

Query: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 60
           +ATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR
Sbjct: 31  VATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 90

Query: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLI 120
           MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVL VVRCLLI
Sbjct: 91  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRCLLI 150

Query: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 180
           EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFK GAISESTTALNVDPTNA
Sbjct: 151 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKAGAISESTTALNVDPTNA 210

Query: 181 SALSTDLKNASATALPLACPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAA 240
           SALSTDLKNASATALPLACPTANS T VRSGQEQQQPNVVTALASESGVG SGVG APAA
Sbjct: 211 SALSTDLKNASATALPLACPTANSTTAVRSGQEQQQPNVVTALASESGVGVSGVGVAPAA 270

Query: 241 MVKKESGHLKMQQVGKKKIDARKKSLKRL 270
            VKKESGHLKMQQV KKKIDARKKSLKRL
Sbjct: 271 TVKKESGHLKMQQVDKKKIDARKKSLKRL 299

BLAST of CSPI04G17180 vs. ExPASy TrEMBL
Match: A0A6J1EAT8 (ubiquitin-conjugating enzyme E2 22-like OS=Cucurbita moschata OX=3662 GN=LOC111432427 PE=3 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 2.8e-125
Identity = 239/269 (88.85%), Postives = 244/269 (90.71%), Query Frame = 0

Query: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 60
           MATNENLPPNVIKQLAKELKSLDESPPEGIKV VNDDDFSTI+ADIEGPVGTPYENGLFR
Sbjct: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVGVNDDDFSTIYADIEGPVGTPYENGLFR 60

Query: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLI 120
           MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVL VVRCLLI
Sbjct: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRCLLI 120

Query: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 180
           EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFK+GAISESTTALNVDPTNA
Sbjct: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKSGAISESTTALNVDPTNA 180

Query: 181 SALSTDLKNASATALPLACPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAA 240
           SAL+TDLKN SA  L  ACPTANS T VR GQE QQPN  T  A ESGVGGSGV    AA
Sbjct: 181 SALNTDLKNTSAAVLSSACPTANSNTAVRGGQE-QQPNTTTPPALESGVGGSGVAAPAAA 240

Query: 241 MVKKESGHLKMQQVGKKKIDARKKSLKRL 270
           +VKK+ G LKMQ   KKKIDARKKSLKRL
Sbjct: 241 VVKKQDGPLKMQG-DKKKIDARKKSLKRL 267

BLAST of CSPI04G17180 vs. NCBI nr
Match: XP_004142654.1 (ubiquitin-conjugating enzyme E2 22 [Cucumis sativus] >KGN54504.1 hypothetical protein Csa_011956 [Cucumis sativus])

HSP 1 Score: 527.3 bits (1357), Expect = 7.6e-146
Identity = 268/269 (99.63%), Postives = 268/269 (99.63%), Query Frame = 0

Query: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 60
           MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR
Sbjct: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 60

Query: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLI 120
           MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLI
Sbjct: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLI 120

Query: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 180
           EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA
Sbjct: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 180

Query: 181 SALSTDLKNASATALPLACPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAA 240
           SALSTDLKNASATALPL CPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAA
Sbjct: 181 SALSTDLKNASATALPLVCPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAA 240

Query: 241 MVKKESGHLKMQQVGKKKIDARKKSLKRL 270
           MVKKESGHLKMQQVGKKKIDARKKSLKRL
Sbjct: 241 MVKKESGHLKMQQVGKKKIDARKKSLKRL 269

BLAST of CSPI04G17180 vs. NCBI nr
Match: XP_008464614.1 (PREDICTED: ubiquitin-conjugating enzyme E2 22 [Cucumis melo])

HSP 1 Score: 511.9 bits (1317), Expect = 3.3e-141
Identity = 262/269 (97.40%), Postives = 262/269 (97.40%), Query Frame = 0

Query: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 60
           MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR
Sbjct: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 60

Query: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLI 120
           MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVL VVRCLLI
Sbjct: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRCLLI 120

Query: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 180
           EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA
Sbjct: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 180

Query: 181 SALSTDLKNASATALPLACPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAA 240
           SALSTDLKNASATALPLACPTANS T VRSGQEQQQPNVVTALASESGVG SGVG APAA
Sbjct: 181 SALSTDLKNASATALPLACPTANSTTAVRSGQEQQQPNVVTALASESGVGVSGVGVAPAA 240

Query: 241 MVKKESGHLKMQQVGKKKIDARKKSLKRL 270
            VKKESGHLKMQQV KKKIDARKKSLKRL
Sbjct: 241 TVKKESGHLKMQQVDKKKIDARKKSLKRL 269

BLAST of CSPI04G17180 vs. NCBI nr
Match: KAA0067923.1 (ubiquitin-conjugating enzyme E2 22 [Cucumis melo var. makuwa])

HSP 1 Score: 510.4 bits (1313), Expect = 9.7e-141
Identity = 261/269 (97.03%), Postives = 262/269 (97.40%), Query Frame = 0

Query: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 60
           +ATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR
Sbjct: 31  VATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 90

Query: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLI 120
           MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVL VVRCLLI
Sbjct: 91  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRCLLI 150

Query: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 180
           EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA
Sbjct: 151 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 210

Query: 181 SALSTDLKNASATALPLACPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAA 240
           SALSTDLKNASATALPLACPTANS T VRSGQEQQQPNVVTALASESGVG SGVG APAA
Sbjct: 211 SALSTDLKNASATALPLACPTANSTTAVRSGQEQQQPNVVTALASESGVGVSGVGVAPAA 270

Query: 241 MVKKESGHLKMQQVGKKKIDARKKSLKRL 270
            VKKESGHLKMQQV KKKIDARKKSLKRL
Sbjct: 271 TVKKESGHLKMQQVDKKKIDARKKSLKRL 299

BLAST of CSPI04G17180 vs. NCBI nr
Match: TYK04292.1 (ubiquitin-conjugating enzyme E2 22 [Cucumis melo var. makuwa])

HSP 1 Score: 508.4 bits (1308), Expect = 3.7e-140
Identity = 260/269 (96.65%), Postives = 261/269 (97.03%), Query Frame = 0

Query: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 60
           +ATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR
Sbjct: 31  VATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 90

Query: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLI 120
           MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVL VVRCLLI
Sbjct: 91  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRCLLI 150

Query: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 180
           EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFK GAISESTTALNVDPTNA
Sbjct: 151 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKAGAISESTTALNVDPTNA 210

Query: 181 SALSTDLKNASATALPLACPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAA 240
           SALSTDLKNASATALPLACPTANS T VRSGQEQQQPNVVTALASESGVG SGVG APAA
Sbjct: 211 SALSTDLKNASATALPLACPTANSTTAVRSGQEQQQPNVVTALASESGVGVSGVGVAPAA 270

Query: 241 MVKKESGHLKMQQVGKKKIDARKKSLKRL 270
            VKKESGHLKMQQV KKKIDARKKSLKRL
Sbjct: 271 TVKKESGHLKMQQVDKKKIDARKKSLKRL 299

BLAST of CSPI04G17180 vs. NCBI nr
Match: XP_038881813.1 (ubiquitin-conjugating enzyme E2 22 [Benincasa hispida])

HSP 1 Score: 460.7 bits (1184), Expect = 8.8e-126
Identity = 243/269 (90.33%), Postives = 248/269 (92.19%), Query Frame = 0

Query: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 60
           MATNENLPPNVIKQLAKELKSLDESPPEGIKV VNDDDFSTIFADIEGPVGTPYENGLFR
Sbjct: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVGVNDDDFSTIFADIEGPVGTPYENGLFR 60

Query: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLI 120
           MKLILS DFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVL VVRCLLI
Sbjct: 61  MKLILSHDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRCLLI 120

Query: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 180
           EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDP N 
Sbjct: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPINT 180

Query: 181 SALSTDLKNASATALPLACPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAA 240
           SAL+TDL N SA ALPLACPTA+S T VR GQEQQQPNVV+  ASESGVGGS  G A AA
Sbjct: 181 SALNTDLINTSA-ALPLACPTAHSNTAVRGGQEQQQPNVVSTPASESGVGGS--GGAVAA 240

Query: 241 MVKKESGHLKMQQVGKKKIDARKKSLKRL 270
           +VKKE+G LKMQ   KKKIDARKKSLKRL
Sbjct: 241 VVKKEAGPLKMQS-DKKKIDARKKSLKRL 265

BLAST of CSPI04G17180 vs. TAIR 10
Match: AT5G05080.1 (ubiquitin-conjugating enzyme 22 )

HSP 1 Score: 363.6 bits (932), Expect = 1.4e-100
Identity = 197/269 (73.23%), Postives = 213/269 (79.18%), Query Frame = 0

Query: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 60
           MA+NENLPPNVIKQLAKELKSLDESPP+GIKVVVND+DFS I ADIEGPVGTPYENGLFR
Sbjct: 1   MASNENLPPNVIKQLAKELKSLDESPPDGIKVVVNDEDFSQICADIEGPVGTPYENGLFR 60

Query: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLI 120
           MKL LS DFP SPPKGYF+TKIFHPN+A+NGEICVNTLKKDWNPSLGLRHVL+VVRCLLI
Sbjct: 61  MKLALSHDFPHSPPKGYFMTKIFHPNVASNGEICVNTLKKDWNPSLGLRHVLSVVRCLLI 120

Query: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 180
           EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNV  TN 
Sbjct: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVGQTNN 180

Query: 181 SALSTDLKNASATALPLACPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAA 240
                     +ATA+P +      +T   S Q+QQ    V   AS S V           
Sbjct: 181 ET------PGAATAIPSSMTDIKRVTT--SAQDQQHVANVVVAASASVV---------TT 240

Query: 241 MVKKESGHLKMQQVGKKKIDARKKSLKRL 270
             K+E+G  K+ Q  KKK+DARKKSLKRL
Sbjct: 241 TQKREAGLAKV-QADKKKVDARKKSLKRL 251

BLAST of CSPI04G17180 vs. TAIR 10
Match: AT5G05080.2 (ubiquitin-conjugating enzyme 22 )

HSP 1 Score: 363.6 bits (932), Expect = 1.4e-100
Identity = 197/269 (73.23%), Postives = 213/269 (79.18%), Query Frame = 0

Query: 1   MATNENLPPNVIKQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFR 60
           MA+NENLPPNVIKQLAKELKSLDESPP+GIKVVVND+DFS I ADIEGPVGTPYENGLFR
Sbjct: 1   MASNENLPPNVIKQLAKELKSLDESPPDGIKVVVNDEDFSQICADIEGPVGTPYENGLFR 60

Query: 61  MKLILSRDFPCSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLI 120
           MKL LS DFP SPPKGYF+TKIFHPN+A+NGEICVNTLKKDWNPSLGLRHVL+VVRCLLI
Sbjct: 61  MKLALSHDFPHSPPKGYFMTKIFHPNVASNGEICVNTLKKDWNPSLGLRHVLSVVRCLLI 120

Query: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVDPTNA 180
           EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNV  TN 
Sbjct: 121 EPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTTALNVGQTNN 180

Query: 181 SALSTDLKNASATALPLACPTANSITVVRSGQEQQQPNVVTALASESGVGGSGVGEAPAA 240
                     +ATA+P +      +T   S Q+QQ    V   AS S V           
Sbjct: 181 ET------PGAATAIPSSMTDIKRVTT--SAQDQQHVANVVVAASASVV---------TT 240

Query: 241 MVKKESGHLKMQQVGKKKIDARKKSLKRL 270
             K+E+G  K+ Q  KKK+DARKKSLKRL
Sbjct: 241 TQKREAGLAKV-QADKKKVDARKKSLKRL 251

BLAST of CSPI04G17180 vs. TAIR 10
Match: AT2G02760.1 (ubiquiting-conjugating enzyme 2 )

HSP 1 Score: 109.0 bits (271), Expect = 6.0e-24
Identity = 50/136 (36.76%), Postives = 78/136 (57.35%), Query Frame = 0

Query: 13  KQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFRMKLILSRDFPCS 72
           K+L ++ K L + PP GI     D++     A I GP  TP++ G F++ L  S D+P  
Sbjct: 7   KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNK 66

Query: 73  PPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLIEPFPESALNEQA 132
           PP   F++++FHPNI  +G IC++ L+  W+P   +  +LT ++ LL +P P S  N +A
Sbjct: 67  PPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEA 126

Query: 133 GKMLLENYDEYARHAR 149
            +M  E+  EY R  R
Sbjct: 127 ARMFSESKREYNRRVR 142

BLAST of CSPI04G17180 vs. TAIR 10
Match: AT1G14400.1 (ubiquitin carrier protein 1 )

HSP 1 Score: 108.6 bits (270), Expect = 7.9e-24
Identity = 50/136 (36.76%), Postives = 78/136 (57.35%), Query Frame = 0

Query: 13  KQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFRMKLILSRDFPCS 72
           K+L ++ K L + PP GI     D++     A I GP  TP++ G F++ L  S D+P  
Sbjct: 7   KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNK 66

Query: 73  PPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLIEPFPESALNEQA 132
           PP   F++++FHPNI  +G IC++ L+  W+P   +  +LT ++ LL +P P S  N +A
Sbjct: 67  PPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEA 126

Query: 133 GKMLLENYDEYARHAR 149
            +M  E+  EY R  R
Sbjct: 127 ARMYSESKREYNRRVR 142

BLAST of CSPI04G17180 vs. TAIR 10
Match: AT1G14400.2 (ubiquitin carrier protein 1 )

HSP 1 Score: 108.6 bits (270), Expect = 7.9e-24
Identity = 50/136 (36.76%), Postives = 78/136 (57.35%), Query Frame = 0

Query: 13  KQLAKELKSLDESPPEGIKVVVNDDDFSTIFADIEGPVGTPYENGLFRMKLILSRDFPCS 72
           K+L ++ K L + PP GI     D++     A I GP  TP++ G F++ L  S D+P  
Sbjct: 7   KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNK 66

Query: 73  PPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLTVVRCLLIEPFPESALNEQA 132
           PP   F++++FHPNI  +G IC++ L+  W+P   +  +LT ++ LL +P P S  N +A
Sbjct: 67  PPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEA 126

Query: 133 GKMLLENYDEYARHAR 149
            +M  E+  EY R  R
Sbjct: 127 ARMYSESKREYNRRVR 142

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FF661.9e-9973.23Ubiquitin-conjugating enzyme E2 22 OS=Arabidopsis thaliana OX=3702 GN=UBC22 PE=1... [more]
Q28F892.1e-5865.13Ubiquitin-conjugating enzyme E2 S OS=Xenopus tropicalis OX=8364 GN=ube2s PE=2 SV... [more]
C1C3R68.1e-5865.56Ubiquitin-conjugating enzyme E2 S OS=Lithobates catesbeianus OX=8400 GN=ube2s PE... [more]
Q8AVU21.8e-5763.82Ubiquitin-conjugating enzyme E2 S-B OS=Xenopus laevis OX=8355 GN=ube2s-b PE=2 SV... [more]
A7SE051.8e-5764.47Ubiquitin-conjugating enzyme E2 S OS=Nematostella vectensis OX=45351 GN=v1g23715... [more]
Match NameE-valueIdentityDescription
A0A0A0L0F53.7e-14699.63UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G340550 PE... [more]
A0A1S3CLW51.6e-14197.40ubiquitin-conjugating enzyme E2 22 OS=Cucumis melo OX=3656 GN=LOC103502453 PE=3 ... [more]
A0A5A7VIJ34.7e-14197.03Ubiquitin-conjugating enzyme E2 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A5D3BZH91.8e-14096.65Ubiquitin-conjugating enzyme E2 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1EAT82.8e-12588.85ubiquitin-conjugating enzyme E2 22-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
XP_004142654.17.6e-14699.63ubiquitin-conjugating enzyme E2 22 [Cucumis sativus] >KGN54504.1 hypothetical pr... [more]
XP_008464614.13.3e-14197.40PREDICTED: ubiquitin-conjugating enzyme E2 22 [Cucumis melo][more]
KAA0067923.19.7e-14197.03ubiquitin-conjugating enzyme E2 22 [Cucumis melo var. makuwa][more]
TYK04292.13.7e-14096.65ubiquitin-conjugating enzyme E2 22 [Cucumis melo var. makuwa][more]
XP_038881813.18.8e-12690.33ubiquitin-conjugating enzyme E2 22 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G05080.11.4e-10073.23ubiquitin-conjugating enzyme 22 [more]
AT5G05080.21.4e-10073.23ubiquitin-conjugating enzyme 22 [more]
AT2G02760.16.0e-2436.76ubiquiting-conjugating enzyme 2 [more]
AT1G14400.17.9e-2436.76ubiquitin carrier protein 1 [more]
AT1G14400.27.9e-2436.76ubiquitin carrier protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 1..96
e-value: 1.0E-6
score: 38.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 185..209
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 1..203
NoneNo IPR availablePANTHERPTHR24068:SF297UBIQUITIN-CONJUGATING ENZYME E2 22-LIKEcoord: 1..203
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 3..90
e-value: 5.7E-24
score: 84.4
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 1..96
score: 27.414246
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 1..91
e-value: 3.46413E-36
score: 121.926
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 1..108
e-value: 2.0E-31
score: 110.6
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 2..99
IPR023313Ubiquitin-conjugating enzyme, active sitePROSITEPS00183UBC_1coord: 23..38

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G17180.1CSPI04G17180.1mRNA
CSPI04G17180.2CSPI04G17180.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0061631 ubiquitin conjugating enzyme activity