CSPI04G16910 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G16910
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionReverse transcriptase domain-containing protein
LocationChr4: 14334274 .. 14335764 (+)
RNA-Seq ExpressionCSPI04G16910
SyntenyCSPI04G16910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTATGCCATTATCTATTGGCTTTTTGAGAAAATATGGGGTAAAATTGAAATGTCTACGATAATGATTCTTGAGAATGAAGTTTTTTGTTTCCAATTTAGACATCCAAAATCTGTTGAGTGGATCTTATCTCGTGGATGGTGGCACCTTAAGGGCAAACATATGCTCCTCCGTACATGGACTCTAAGTTAGTCTCTGAATCCTTTGTTTTCAATTCTATTCCTAAATGGATAAAACTTGGTAGGATTCCTATGGAGTTATGGATAAATATCAGATTGGATGTTGTTGCTAGTGCTATAGGCAAACATCTATCTTTAGATCTAGCTACTAAGGAAAGACGAAAACTTTCATTTGCGCGAGTTTGTGTTGAATTAAATGTGGAGTCTCCTATGCTTGCTGAAATTACAGTTAACCTTAGGAGAGTGGATTTCATTGTGACTGTGAATTATGAATGGAAGCCTCGAAAATGTAATTTGTACCGTGCCTTTAGGCATTGTAGTGGTAAGTGCCCTAGGATTGTGGAGAATAAAGTACATCATGAGGAGGAAGTGAGGAAGGTAGTTTTTAACAAAGAGGATGATCTCAATAATGTGGCGTGTGGAGATGTGGTGTTTGAGTCATTTAAATAGTTGGAGGAAGGTGAGATTAATAGCTCCCTCATTAGGCCTACAAAGGAAAAGGGGGTGGGTAATCAGGATGATTTTACTCTTGTCATTGGGAAGAAATGGGAGTTGGTTTATGTTCGATATAAGGGTAAAAGTGTGGATGTGACTATGCCTAACTCTTTTGGCAACCTTATGGAGTTGGGTAAGGGAGACAAGTGGGCCTTGTCTATAGTGGAAGGCTCCGCTCTTCCCTTACAAGTGGATGATACCACTATGGTTCTTAAGGGCATGCGCTCAGATGGTAATCCTGCGGGAACGAAGGCTTCTAATTCTTCTCATGATTAGTTAGTGTTCTTGGAATGTGAGGGGTCTAAATAGCGTTGTAAAGTGTAGAGCGGTGCTTAATTTTTTGGGTGTGTCTTCTGTGGGTTTCTGTTGTCTTTTGAAAACTAGAGTTCGGGAAGAAAACTTTGGTTTTATCTCCAATAGGTTTGGAAATTCGTGGGATTTCACTAGTAACTATAGTAGCAGTGAGATTGGTCGTATGTGGGTAATGTGGAAGAAGAGTCGCTTCGTTCTTACTCCTGGTGAGGAGGAAGATCAGTTTGTCTCTAGGTCATTAACTGATCTACTATCTGGGGATTGTGTAGAGGTACTGTGTGTTTATGCCTTTAATAATAATGTTGAGAGACGTTTGTTGTGGCGTCGTATGGGTGAGATCTCTTCTAGATGGACGAGGCCAAATGATGTCATTGGTGACTTTAATGCCATTAGGCTTCATTCTGAAGCTTTTGGTGGATCTTCTGTTACTGGTTATATAGAGGAGTTCGATATGGTCATTCGTGAGGCTGACCTAGTTGAACCATCAATACAGGGCAACTAG

mRNA sequence

ATGTCTTATGCCATTATCTATTGGCTTTTTGAGAAAATATGGGGGCAAACATATGCTCCTCCGTACATGGACTCTAAGTTAGTCTCTGAATCCTTTGTTTTCAATTCTATTCCTAAATGGATAAAACTTGGTAGGATTCCTATGGAGTTATGGATAAATATCAGATTGGATGTTGTTGCTAGTGCTATAGGCAAACATCTATCTTTAGATCTAGCTACTAAGGAAAGACGAAAACTTTCATTTGCGCGAGTTTGTGTTGAATTAAATGTGGAGTCTCCTATGCTTGCTGAAATTACAGTTAACCTTAGGAGAGTGGATTTCATTGTGACTGTGAATTATGAATGGAAGCCTCGAAAATGTAATTTGTACCGTGCCTTTAGGCATTGTAGTGGTAAGTGCCCTAGGATTGTGGAGAATAAAGTACATCATGAGGAGGAAGTGAGGAAGTTGGAGGAAGGTGAGATTAATAGCTCCCTCATTAGGCCTACAAAGGAAAAGGGGGTGGGTAATCAGGATGATTTTACTCTTGTCATTGGGAAGAAATGGGAGTTGGTTTATGTTCGATATAAGGGTAAAAGTGTGGATGTGACTATGCCTAACTCTTTTGGCAACCTTATGGAGTTGGGTAAGGGAGACAAGTGGGCCTTGTCTATAGTGGAAGGCTCCGCTCTTCCCTTACAAGTGGATGATACCACTATGGTTCTTAAGGGCATGCGCTCAGATGGTAATCCTGCGGGAACGAAGGCTTCTAATTCTTCTCATGATTACGTTGTAAAGTGTAGAGCGGTGCTTAATTTTTTGGGTGTGTCTTCTGTGGGTTTCTGTTGTCTTTTGAAAACTAGAGTTCGGGAAGAAAACTTTGGTTTTATCTCCAATAGGTTTGGAAATTCGTGGGATTTCACTAGTAACTATAGTAGCAGTGAGATTGGTCGTATGTGGGTAATGTGGAAGAAGAGTCGCTTCGTTCTTACTCCTGGTGAGGAGGAAGATCAGTTTGTCTCTAGGTCATTAACTGATCTACTATCTGGGGATTGTGTAGAGGTACTGTGTGTTTATGCCTTTAATAATAATGTTGAGAGACGTTTGTTGTGGCGTCGTATGGGTGAGATCTCTTCTAGATGGACGAGGCCAAATGATGTCATTGGTGACTTTAATGCCATTAGGCTTCATTCTGAAGCTTTTGGTGGATCTTCTGTTACTGGTTATATAGAGGAGTTCGATATGGTCATTCGTGAGGCTGACCTAGTTGAACCATCAATACAGGGCAACTAG

Coding sequence (CDS)

ATGTCTTATGCCATTATCTATTGGCTTTTTGAGAAAATATGGGGGCAAACATATGCTCCTCCGTACATGGACTCTAAGTTAGTCTCTGAATCCTTTGTTTTCAATTCTATTCCTAAATGGATAAAACTTGGTAGGATTCCTATGGAGTTATGGATAAATATCAGATTGGATGTTGTTGCTAGTGCTATAGGCAAACATCTATCTTTAGATCTAGCTACTAAGGAAAGACGAAAACTTTCATTTGCGCGAGTTTGTGTTGAATTAAATGTGGAGTCTCCTATGCTTGCTGAAATTACAGTTAACCTTAGGAGAGTGGATTTCATTGTGACTGTGAATTATGAATGGAAGCCTCGAAAATGTAATTTGTACCGTGCCTTTAGGCATTGTAGTGGTAAGTGCCCTAGGATTGTGGAGAATAAAGTACATCATGAGGAGGAAGTGAGGAAGTTGGAGGAAGGTGAGATTAATAGCTCCCTCATTAGGCCTACAAAGGAAAAGGGGGTGGGTAATCAGGATGATTTTACTCTTGTCATTGGGAAGAAATGGGAGTTGGTTTATGTTCGATATAAGGGTAAAAGTGTGGATGTGACTATGCCTAACTCTTTTGGCAACCTTATGGAGTTGGGTAAGGGAGACAAGTGGGCCTTGTCTATAGTGGAAGGCTCCGCTCTTCCCTTACAAGTGGATGATACCACTATGGTTCTTAAGGGCATGCGCTCAGATGGTAATCCTGCGGGAACGAAGGCTTCTAATTCTTCTCATGATTACGTTGTAAAGTGTAGAGCGGTGCTTAATTTTTTGGGTGTGTCTTCTGTGGGTTTCTGTTGTCTTTTGAAAACTAGAGTTCGGGAAGAAAACTTTGGTTTTATCTCCAATAGGTTTGGAAATTCGTGGGATTTCACTAGTAACTATAGTAGCAGTGAGATTGGTCGTATGTGGGTAATGTGGAAGAAGAGTCGCTTCGTTCTTACTCCTGGTGAGGAGGAAGATCAGTTTGTCTCTAGGTCATTAACTGATCTACTATCTGGGGATTGTGTAGAGGTACTGTGTGTTTATGCCTTTAATAATAATGTTGAGAGACGTTTGTTGTGGCGTCGTATGGGTGAGATCTCTTCTAGATGGACGAGGCCAAATGATGTCATTGGTGACTTTAATGCCATTAGGCTTCATTCTGAAGCTTTTGGTGGATCTTCTGTTACTGGTTATATAGAGGAGTTCGATATGGTCATTCGTGAGGCTGACCTAGTTGAACCATCAATACAGGGCAACTAG

Protein sequence

MSYAIIYWLFEKIWGQTYAPPYMDSKLVSESFVFNSIPKWIKLGRIPMELWINIRLDVVASAIGKHLSLDLATKERRKLSFARVCVELNVESPMLAEITVNLRRVDFIVTVNYEWKPRKCNLYRAFRHCSGKCPRIVENKVHHEEEVRKLEEGEINSSLIRPTKEKGVGNQDDFTLVIGKKWELVYVRYKGKSVDVTMPNSFGNLMELGKGDKWALSIVEGSALPLQVDDTTMVLKGMRSDGNPAGTKASNSSHDYVVKCRAVLNFLGVSSVGFCCLLKTRVREENFGFISNRFGNSWDFTSNYSSSEIGRMWVMWKKSRFVLTPGEEEDQFVSRSLTDLLSGDCVEVLCVYAFNNNVERRLLWRRMGEISSRWTRPNDVIGDFNAIRLHSEAFGGSSVTGYIEEFDMVIREADLVEPSIQGN*
Homology
BLAST of CSPI04G16910 vs. ExPASy TrEMBL
Match: A0A5A7V5J2 (Non-LTR retroelement reverse transcriptase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold387G00210 PE=4 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 7.9e-127
Identity = 239/423 (56.50%), Postives = 297/423 (70.21%), Query Frame = 0

Query: 27  LVSESFVFNSIPKWIKLGRIPMELWINIRLDVVASAIGKHLSLDLATKERRKLSFARVCV 86
           +V ESFVFNS+P WI+LGRIPMELW    L +VAS +GK ++LDLATKE  +LS+ARVCV
Sbjct: 47  IVPESFVFNSVPVWIRLGRIPMELWTEAWLAIVASVVGKPITLDLATKEHSRLSYARVCV 106

Query: 87  ELNVESPMLAEITVNLRRVDFIVTVNYEWKPRKCNLYRAFRHCSGKCPRIVENKVHHEEE 146
           +L  E  M AEITVNLR VDF V+VNYEWKP+KCNL  A  H  GKCPR VE+K+  EE 
Sbjct: 107 KLEGEFNMRAEITVNLRGVDFNVSVNYEWKPQKCNLCCAIGHSGGKCPRSVESKIIQEEV 166

Query: 147 VRK------------------------LEEGEINSSLIRPTK--EKGVGNQDDFTLVIGK 206
           V K                        LEEGEI SS  R     EK VG +D+FTLV  K
Sbjct: 167 VSKIIPGKGEGVDSEPCAKVVLESFKQLEEGEIRSSPNRHGSHVEKEVGKRDEFTLVTRK 226

Query: 207 KWELVYVRYKGKSVDVTMPNSFGNLMELGKGDKWALSIVEGSALPLQVDDTTMVLKGMRS 266
           K ELV VR +GKS+ V MPNSFG+L+E+G  DKWAL+++EGS  PLQVD+ T VL  ++S
Sbjct: 227 KSELVSVRDRGKSIVVAMPNSFGSLLEVGDVDKWALTVIEGSPPPLQVDEGTGVLSDLKS 286

Query: 267 DGNPAGTKASNSSHDYVVKCRAVLNFLGVSSVGFCCLLKTRVREENFGFISNRFGNSWDF 326
                               +AV +FLG SSVGFCCLL+TRVRE NF ++S RFGNSWD+
Sbjct: 287 --------------------KAVSDFLGSSSVGFCCLLETRVRESNFDYVSRRFGNSWDY 346

Query: 327 TSNYSSSEIGRMWVMWKKSRFVLTPGEEEDQFVSRSLTDLLSGDCVEVLCVYAFNNNVER 386
           + +YS+S +GR+WV+WKK+RF  +    ++QFV+ +LTDLLSG CVEV CVYA N+N+ER
Sbjct: 347 SCSYSNSGVGRIWVVWKKNRFSFSTCVVDEQFVTGTLTDLLSGVCVEVFCVYASNSNIER 406

Query: 387 RLLWRRMGEISSRWTRPNDVIGDFNAIRLHSEAFGGSSVTGYIEEFDMVIREADLVEPSI 424
           RLLWRR+ EI+S W+ P  V+GDFNAIR+H EAFGGS + G +E+FD+  R+ADLVEPS+
Sbjct: 407 RLLWRRLVEITSCWSSPEVVMGDFNAIRVHFEAFGGSPIQGEMEDFDLATRDADLVEPSV 449

BLAST of CSPI04G16910 vs. ExPASy TrEMBL
Match: A0A5A7U3C2 (Exo_endo_phos domain-containing protein/DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00410 PE=4 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 1.6e-119
Identity = 227/397 (57.18%), Postives = 279/397 (70.28%), Query Frame = 0

Query: 48  MELWINIRLDVVASAIGKHLSLDLATKERRKLSFARVCVELNVESPMLAEITVNLRRVDF 107
           M+LW    L VVAS +GK +SLDLA K+R +LS+ARVC+EL   S M AEITV+LR VDF
Sbjct: 1   MKLWTEAGLAVVASVVGKPISLDLANKKRHRLSYARVCIELEGGSNMPAEITVSLRGVDF 60

Query: 108 IVTVNYEWKPRKCNLYRAFRHCSGKCPRIVENKVHHEEEVRK------------------ 167
            V+VNYEWKPRKCNL  AF H   KC R VE+K   EE V K                  
Sbjct: 61  NVSVNYEWKPRKCNLCCAFGHSGSKCSRSVESKTIQEEVVHKGDGLDSEPCGEVVLESFK 120

Query: 168 -LEEGEINSSLIRPTK--EKGVGNQDDFTLVIGKKWELVYVRYKGKSVDVTMPNSFGNLM 227
            LEEG+I SS  R     EKGVG  DDFTLV  KK ELV VR +GKS++V MPNSFG+L+
Sbjct: 121 QLEEGKIRSSPNRHNSQVEKGVGKSDDFTLVTRKKSELVSVRDRGKSMEVIMPNSFGSLL 180

Query: 228 ELGKGDKWALSIVEGSALPLQVDDTTMVLKGMRSDGNPAGTKASNSSHDYVVKCRAVLNF 287
           E+G  DKW LSI+EGS  PLQVD+ T VL GM+S                    +AV++F
Sbjct: 181 EVGDADKWVLSIIEGSPPPLQVDEDTDVLSGMKS--------------------KAVVDF 240

Query: 288 LGVSSVGFCCLLKTRVREENFGFISNRFGNSWDFTSNYSSSEIGRMWVMWKKSRFVLTPG 347
           LG SS+GFCCLL+TRVRE NF  +S RFGNSWD++ +YS+S +GR+WVMWKK+RF  +  
Sbjct: 241 LGSSSMGFCCLLETRVREGNFASVSRRFGNSWDYSCSYSNSGVGRIWVMWKKNRFSFSTH 300

Query: 348 EEEDQFVSRSLTDLLSGDCVEVLCVYAFNNNVERRLLWRRMGEISSRWTRPNDVIGDFNA 407
             ++QFV+ +LTDLLSG CVEV CVYA N+N+ERRLLWRR+ EI+S W+ P  V+GDFNA
Sbjct: 301 VMDEQFVTGTLTDLLSGVCVEVFCVYASNSNIERRLLWRRLVEITSAWSNPGVVMGDFNA 360

Query: 408 IRLHSEAFGGSSVTGYIEEFDMVIREADLVEPSIQGN 424
           IR+HSEAFGGS + G +E+FD+ IR+ADL+EPS+QGN
Sbjct: 361 IRVHSEAFGGSPIQGEMEDFDLAIRDADLIEPSVQGN 377

BLAST of CSPI04G16910 vs. ExPASy TrEMBL
Match: A0A5A7V084 (Exo_endo_phos domain-containing protein/DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G00100 PE=4 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 1.8e-118
Identity = 228/410 (55.61%), Postives = 284/410 (69.27%), Query Frame = 0

Query: 37  IPKWIKLGRIPMELWINIRLDVVASAIGKHLSLDLATKERRKLSFARVCVELNVESPMLA 96
           +P WI+LGRIPMELW    L VVASA+GK ++LDLATKERR+LS+ARVCV+L+ +S M A
Sbjct: 191 VPVWIRLGRIPMELWTEAGLAVVASAVGKPITLDLATKERRRLSYARVCVKLDGDSHMPA 250

Query: 97  EITVNLRRVDFIVTVNYEWKPRKCNLYRAFRHCSGKCPRIVENKVHHEEEVRK------- 156
           EITV+LR VDFIV++NYEWKP KCNL   F H  GKC R VE+KV  EE V K       
Sbjct: 251 EITVSLRGVDFIVSINYEWKPWKCNLCCVFGHSGGKCHRNVESKVIQEEVVSKVSPSKGD 310

Query: 157 -----------------LEEGEINSSLIRPTK--EKGVGNQDDFTLVIGKKWELVYVRYK 216
                            LEEGEI SS  R +   EK VG +DDFTLV  KK ELV+VR +
Sbjct: 311 GLDSEPCGEVVLESFKQLEEGEIRSSRNRHSSQVEKEVGKRDDFTLVTRKKRELVFVRDR 370

Query: 217 GKSVDVTMPNSFGNLMELGKGDKWALSIVEGSALPLQVDDTTMVLKGMRSDGNPAGTKAS 276
           GKS++VTMPN FG+L+E+G  DKWAL+I++GS   LQVD+ T VL G             
Sbjct: 371 GKSLEVTMPNFFGSLLEVGDFDKWALTIIDGSLSALQVDEGTGVLSG------------- 430

Query: 277 NSSHDYVVKCRAVLNFLGVSSVGFCCLLKTRVREENFGFISNRFGNSWDFTSNYSSSEIG 336
                  VKC+ V +FLG SSVGFCCLL++R RE NF  +S RFG SWD++ +YS+S +G
Sbjct: 431 -------VKCKVVFDFLGSSSVGFCCLLESRFRERNFESVSKRFGYSWDYSCSYSNSGVG 490

Query: 337 RMWVMWKKSRFVLTPGEEEDQFVSRSLTDLLSGDCVEVLCVYAFNNNVERRLLWRRMGEI 396
           R+WVMWKK+RF  +    ++QFV  +LTDLLS  CVEV CVYA  +N+ERRLLWRR+ EI
Sbjct: 491 RIWVMWKKNRFSFSTRMVDEQFVIGTLTDLLSDVCVEVFCVYASYSNIERRLLWRRLVEI 550

Query: 397 SSRWTRPNDVIGDFNAIRLHSEAFGGSSVTGYIEEFDMVIREADLVEPSI 421
           +S W+ P  V+GDFN IR+HSEAFGGS   G +E+FD+ I +ADL+EPS+
Sbjct: 551 TSGWSSPGVVMGDFNVIRVHSEAFGGSPNQGEMEDFDLTILDADLIEPSV 580

BLAST of CSPI04G16910 vs. ExPASy TrEMBL
Match: A0A5A7SPE5 (Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold581G00620 PE=4 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 3.4e-106
Identity = 209/380 (55.00%), Postives = 258/380 (67.89%), Query Frame = 0

Query: 65  KHLSLDLATKERRKLSFARVCVELNVESPMLAEITVNLRRVDFIVTVNYEWKPRKCNLYR 124
           K +SLD ATK+RR+LS+ARVCVEL   S M AEITV+LR VDF V+VNYEWKPRKCNL  
Sbjct: 256 KPISLDFATKKRRRLSYARVCVELEGGSNMSAEITVSLRGVDFNVSVNYEWKPRKCNLCC 315

Query: 125 AFRHCSGKCPRIVENKVHHEEEVRK-------------------LEEGEINSSLIRPTK- 184
           AF H S KC R VE+K   EE V K                   +E+GEI SS  R    
Sbjct: 316 AFGHSSSKCSRSVESKTIQEEVVHKGDDVDFELCGEVVLESFKQVEKGEIRSSPNRHDSQ 375

Query: 185 -EKGVGNQDDFTLVIGKKWELVYVRYKGKSVDVTMPNSFGNLMELGKGDKWALSIVEGSA 244
            EKGVG  D+FTLV  KK ELV +R +GKS++V MPNSFG+L+E+G  DKWALSI+EGS+
Sbjct: 376 VEKGVGKSDEFTLVTRKKSELVSIRDRGKSMEVIMPNSFGSLLEVGDADKWALSIIEGSS 435

Query: 245 LPLQVDDTTMVLKGMRSDGNPAGTKASNSSHDYVVKCRAVLNFLGVSSVGFCCLLKTRVR 304
            PLQVD+ T VL G+ S                             S VGFCCLL+TRVR
Sbjct: 436 PPLQVDEGTDVLSGISS-----------------------------SIVGFCCLLETRVR 495

Query: 305 EENFGFISNRFGNSWDFTSNYSSSEIGRMWVMWKKSRFVLTPGEEEDQFVSRSLTDLLSG 364
           E NF  +S RF NSWD++ +YS+S +GR+WVMWKK+RF  +    ++QFV+ +LTDLLSG
Sbjct: 496 EGNFDSVSRRFSNSWDYSCSYSNSGVGRIWVMWKKNRFSFSTHVMDEQFVTGTLTDLLSG 555

Query: 365 DCVEVLCVYAFNNNVERRLLWRRMGEISSRWTRPNDVIGDFNAIRLHSEAFGGSSVTGYI 424
            CVEV CVYA N+N+ERRLLW R+ EI+S W+ P  V+ DFNAIR+HSEAF GS + G +
Sbjct: 556 VCVEVFCVYASNSNIERRLLWHRLVEITSAWSSPGVVMEDFNAIRVHSEAFRGSPIQGEM 606

BLAST of CSPI04G16910 vs. ExPASy TrEMBL
Match: A0A5D3DXE4 (Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1467G00020 PE=4 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 3.4e-106
Identity = 209/380 (55.00%), Postives = 258/380 (67.89%), Query Frame = 0

Query: 65  KHLSLDLATKERRKLSFARVCVELNVESPMLAEITVNLRRVDFIVTVNYEWKPRKCNLYR 124
           K +SLD ATK+RR+LS+ARVCVEL   S M AEITV+LR VDF V+VNYEWKPRKCNL  
Sbjct: 256 KPISLDFATKKRRRLSYARVCVELEGGSNMSAEITVSLRGVDFNVSVNYEWKPRKCNLCC 315

Query: 125 AFRHCSGKCPRIVENKVHHEEEVRK-------------------LEEGEINSSLIRPTK- 184
           AF H S KC R VE+K   EE V K                   +E+GEI SS  R    
Sbjct: 316 AFGHSSSKCSRSVESKTIQEEVVHKGDDVDFELCGEVVLESFKQVEKGEIRSSPNRHDSQ 375

Query: 185 -EKGVGNQDDFTLVIGKKWELVYVRYKGKSVDVTMPNSFGNLMELGKGDKWALSIVEGSA 244
            EKGVG  D+FTLV  KK ELV +R +GKS++V MPNSFG+L+E+G  DKWALSI+EGS+
Sbjct: 376 VEKGVGKSDEFTLVTRKKSELVSIRDRGKSMEVIMPNSFGSLLEVGDADKWALSIIEGSS 435

Query: 245 LPLQVDDTTMVLKGMRSDGNPAGTKASNSSHDYVVKCRAVLNFLGVSSVGFCCLLKTRVR 304
            PLQVD+ T VL G+ S                             S VGFCCLL+TRVR
Sbjct: 436 PPLQVDEGTDVLSGISS-----------------------------SIVGFCCLLETRVR 495

Query: 305 EENFGFISNRFGNSWDFTSNYSSSEIGRMWVMWKKSRFVLTPGEEEDQFVSRSLTDLLSG 364
           E NF  +S RF NSWD++ +YS+S +GR+WVMWKK+RF  +    ++QFV+ +LTDLLSG
Sbjct: 496 EGNFDSVSRRFSNSWDYSCSYSNSGVGRIWVMWKKNRFSFSTHVMDEQFVTGTLTDLLSG 555

Query: 365 DCVEVLCVYAFNNNVERRLLWRRMGEISSRWTRPNDVIGDFNAIRLHSEAFGGSSVTGYI 424
            CVEV CVYA N+N+ERRLLW R+ EI+S W+ P  V+ DFNAIR+HSEAF GS + G +
Sbjct: 556 VCVEVFCVYASNSNIERRLLWHRLVEITSAWSSPGVVMEDFNAIRVHSEAFRGSPIQGEM 606

BLAST of CSPI04G16910 vs. NCBI nr
Match: XP_004142722.2 (uncharacterized protein LOC101204295 [Cucumis sativus])

HSP 1 Score: 721.5 bits (1861), Expect = 4.3e-204
Identity = 361/401 (90.02%), Postives = 361/401 (90.02%), Query Frame = 0

Query: 23  MDSKLVSESFVFNSIPKWIKLGRIPMELWINIRLDVVASAIGKHLSLDLATKERRKLSFA 82
           MDSKLVSESFVFNSIPKWIKLGRIPMELWINIRLDVVASAIGKHLSLDLATKERRKLSFA
Sbjct: 1   MDSKLVSESFVFNSIPKWIKLGRIPMELWINIRLDVVASAIGKHLSLDLATKERRKLSFA 60

Query: 83  RVCVELNVESPMLAEITVNLRRVDFIVTVNYEWKPRKCNLYRAFRHCSGKCPRIVENKVH 142
           RVCVELNVESPMLAEITVNLRRVDFIVTVNYEWKPRKCNLYRAFRHCSGKCPRIVENKVH
Sbjct: 61  RVCVELNVESPMLAEITVNLRRVDFIVTVNYEWKPRKCNLYRAFRHCSGKCPRIVENKVH 120

Query: 143 HEEEVRKLEEGEINSSLIRPTKEKGVGNQDDFTLVIGKKWELVYVRYKGKSVDVTMPNSF 202
           HEEEVRKLEEGEINSSLIRPTKEKGVGNQDDFTLVIGKKWELVYVRYKGKSVDVTMPNSF
Sbjct: 121 HEEEVRKLEEGEINSSLIRPTKEKGVGNQDDFTLVIGKKWELVYVRYKGKSVDVTMPNSF 180

Query: 203 GNLMELGKGDKWALSIVEGSALPLQVDDTTMVLKGMRSDGNPAGTKASNSSHDYVVKCRA 262
           GNLMELGKGDKWALSIVEGSALPLQVDDTTMVLKGMRSD                     
Sbjct: 181 GNLMELGKGDKWALSIVEGSALPLQVDDTTMVLKGMRSD--------------------- 240

Query: 263 VLNFLGVSSVGFCCLLKTRVREENFGFISNRFGNSWDFTSNYSSSEIGRMWVMWKKSRFV 322
                              VREENFGFISNRFGNSWDFTSNYSSSEIGRMWVMWKKSRFV
Sbjct: 241 -------------------VREENFGFISNRFGNSWDFTSNYSSSEIGRMWVMWKKSRFV 300

Query: 323 LTPGEEEDQFVSRSLTDLLSGDCVEVLCVYAFNNNVERRLLWRRMGEISSRWTRPNDVIG 382
           LTPGEEEDQFVSRSLTDLLSGDCVEVLCVYAFNNNVERRLLWRRMGEISSRWTRPNDVIG
Sbjct: 301 LTPGEEEDQFVSRSLTDLLSGDCVEVLCVYAFNNNVERRLLWRRMGEISSRWTRPNDVIG 360

Query: 383 DFNAIRLHSEAFGGSSVTGYIEEFDMVIREADLVEPSIQGN 424
           DFNAIRLHSEAFGGSSVTGYIEEFDMVIREADLVEPSIQGN
Sbjct: 361 DFNAIRLHSEAFGGSSVTGYIEEFDMVIREADLVEPSIQGN 361

BLAST of CSPI04G16910 vs. NCBI nr
Match: KAA0062888.1 (non-LTR retroelement reverse transcriptase-like protein [Cucumis melo var. makuwa] >TYK22520.1 non-LTR retroelement reverse transcriptase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 463.8 bits (1192), Expect = 1.6e-126
Identity = 239/423 (56.50%), Postives = 297/423 (70.21%), Query Frame = 0

Query: 27  LVSESFVFNSIPKWIKLGRIPMELWINIRLDVVASAIGKHLSLDLATKERRKLSFARVCV 86
           +V ESFVFNS+P WI+LGRIPMELW    L +VAS +GK ++LDLATKE  +LS+ARVCV
Sbjct: 47  IVPESFVFNSVPVWIRLGRIPMELWTEAWLAIVASVVGKPITLDLATKEHSRLSYARVCV 106

Query: 87  ELNVESPMLAEITVNLRRVDFIVTVNYEWKPRKCNLYRAFRHCSGKCPRIVENKVHHEEE 146
           +L  E  M AEITVNLR VDF V+VNYEWKP+KCNL  A  H  GKCPR VE+K+  EE 
Sbjct: 107 KLEGEFNMRAEITVNLRGVDFNVSVNYEWKPQKCNLCCAIGHSGGKCPRSVESKIIQEEV 166

Query: 147 VRK------------------------LEEGEINSSLIRPTK--EKGVGNQDDFTLVIGK 206
           V K                        LEEGEI SS  R     EK VG +D+FTLV  K
Sbjct: 167 VSKIIPGKGEGVDSEPCAKVVLESFKQLEEGEIRSSPNRHGSHVEKEVGKRDEFTLVTRK 226

Query: 207 KWELVYVRYKGKSVDVTMPNSFGNLMELGKGDKWALSIVEGSALPLQVDDTTMVLKGMRS 266
           K ELV VR +GKS+ V MPNSFG+L+E+G  DKWAL+++EGS  PLQVD+ T VL  ++S
Sbjct: 227 KSELVSVRDRGKSIVVAMPNSFGSLLEVGDVDKWALTVIEGSPPPLQVDEGTGVLSDLKS 286

Query: 267 DGNPAGTKASNSSHDYVVKCRAVLNFLGVSSVGFCCLLKTRVREENFGFISNRFGNSWDF 326
                               +AV +FLG SSVGFCCLL+TRVRE NF ++S RFGNSWD+
Sbjct: 287 --------------------KAVSDFLGSSSVGFCCLLETRVRESNFDYVSRRFGNSWDY 346

Query: 327 TSNYSSSEIGRMWVMWKKSRFVLTPGEEEDQFVSRSLTDLLSGDCVEVLCVYAFNNNVER 386
           + +YS+S +GR+WV+WKK+RF  +    ++QFV+ +LTDLLSG CVEV CVYA N+N+ER
Sbjct: 347 SCSYSNSGVGRIWVVWKKNRFSFSTCVVDEQFVTGTLTDLLSGVCVEVFCVYASNSNIER 406

Query: 387 RLLWRRMGEISSRWTRPNDVIGDFNAIRLHSEAFGGSSVTGYIEEFDMVIREADLVEPSI 424
           RLLWRR+ EI+S W+ P  V+GDFNAIR+H EAFGGS + G +E+FD+  R+ADLVEPS+
Sbjct: 407 RLLWRRLVEITSCWSSPEVVMGDFNAIRVHFEAFGGSPIQGEMEDFDLATRDADLVEPSV 449

BLAST of CSPI04G16910 vs. NCBI nr
Match: KAA0049770.1 (hypothetical protein E6C27_scaffold76G001230 [Cucumis melo var. makuwa] >TYK12107.1 Exo_endo_phos domain-containing protein/DUF4283 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 439.5 bits (1129), Expect = 3.3e-119
Identity = 227/397 (57.18%), Postives = 279/397 (70.28%), Query Frame = 0

Query: 48  MELWINIRLDVVASAIGKHLSLDLATKERRKLSFARVCVELNVESPMLAEITVNLRRVDF 107
           M+LW    L VVAS +GK +SLDLA K+R +LS+ARVC+EL   S M AEITV+LR VDF
Sbjct: 1   MKLWTEAGLAVVASVVGKPISLDLANKKRHRLSYARVCIELEGGSNMPAEITVSLRGVDF 60

Query: 108 IVTVNYEWKPRKCNLYRAFRHCSGKCPRIVENKVHHEEEVRK------------------ 167
            V+VNYEWKPRKCNL  AF H   KC R VE+K   EE V K                  
Sbjct: 61  NVSVNYEWKPRKCNLCCAFGHSGSKCSRSVESKTIQEEVVHKGDGLDSEPCGEVVLESFK 120

Query: 168 -LEEGEINSSLIRPTK--EKGVGNQDDFTLVIGKKWELVYVRYKGKSVDVTMPNSFGNLM 227
            LEEG+I SS  R     EKGVG  DDFTLV  KK ELV VR +GKS++V MPNSFG+L+
Sbjct: 121 QLEEGKIRSSPNRHNSQVEKGVGKSDDFTLVTRKKSELVSVRDRGKSMEVIMPNSFGSLL 180

Query: 228 ELGKGDKWALSIVEGSALPLQVDDTTMVLKGMRSDGNPAGTKASNSSHDYVVKCRAVLNF 287
           E+G  DKW LSI+EGS  PLQVD+ T VL GM+S                    +AV++F
Sbjct: 181 EVGDADKWVLSIIEGSPPPLQVDEDTDVLSGMKS--------------------KAVVDF 240

Query: 288 LGVSSVGFCCLLKTRVREENFGFISNRFGNSWDFTSNYSSSEIGRMWVMWKKSRFVLTPG 347
           LG SS+GFCCLL+TRVRE NF  +S RFGNSWD++ +YS+S +GR+WVMWKK+RF  +  
Sbjct: 241 LGSSSMGFCCLLETRVREGNFASVSRRFGNSWDYSCSYSNSGVGRIWVMWKKNRFSFSTH 300

Query: 348 EEEDQFVSRSLTDLLSGDCVEVLCVYAFNNNVERRLLWRRMGEISSRWTRPNDVIGDFNA 407
             ++QFV+ +LTDLLSG CVEV CVYA N+N+ERRLLWRR+ EI+S W+ P  V+GDFNA
Sbjct: 301 VMDEQFVTGTLTDLLSGVCVEVFCVYASNSNIERRLLWRRLVEITSAWSNPGVVMGDFNA 360

Query: 408 IRLHSEAFGGSSVTGYIEEFDMVIREADLVEPSIQGN 424
           IR+HSEAFGGS + G +E+FD+ IR+ADL+EPS+QGN
Sbjct: 361 IRVHSEAFGGSPIQGEMEDFDLAIRDADLIEPSVQGN 377

BLAST of CSPI04G16910 vs. NCBI nr
Match: KAA0060724.1 (hypothetical protein E6C27_scaffold72G00110 [Cucumis melo var. makuwa] >TYK01466.1 Exo_endo_phos domain-containing protein/DUF4283 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 436.0 bits (1120), Expect = 3.6e-118
Identity = 228/410 (55.61%), Postives = 284/410 (69.27%), Query Frame = 0

Query: 37  IPKWIKLGRIPMELWINIRLDVVASAIGKHLSLDLATKERRKLSFARVCVELNVESPMLA 96
           +P WI+LGRIPMELW    L VVASA+GK ++LDLATKERR+LS+ARVCV+L+ +S M A
Sbjct: 191 VPVWIRLGRIPMELWTEAGLAVVASAVGKPITLDLATKERRRLSYARVCVKLDGDSHMPA 250

Query: 97  EITVNLRRVDFIVTVNYEWKPRKCNLYRAFRHCSGKCPRIVENKVHHEEEVRK------- 156
           EITV+LR VDFIV++NYEWKP KCNL   F H  GKC R VE+KV  EE V K       
Sbjct: 251 EITVSLRGVDFIVSINYEWKPWKCNLCCVFGHSGGKCHRNVESKVIQEEVVSKVSPSKGD 310

Query: 157 -----------------LEEGEINSSLIRPTK--EKGVGNQDDFTLVIGKKWELVYVRYK 216
                            LEEGEI SS  R +   EK VG +DDFTLV  KK ELV+VR +
Sbjct: 311 GLDSEPCGEVVLESFKQLEEGEIRSSRNRHSSQVEKEVGKRDDFTLVTRKKRELVFVRDR 370

Query: 217 GKSVDVTMPNSFGNLMELGKGDKWALSIVEGSALPLQVDDTTMVLKGMRSDGNPAGTKAS 276
           GKS++VTMPN FG+L+E+G  DKWAL+I++GS   LQVD+ T VL G             
Sbjct: 371 GKSLEVTMPNFFGSLLEVGDFDKWALTIIDGSLSALQVDEGTGVLSG------------- 430

Query: 277 NSSHDYVVKCRAVLNFLGVSSVGFCCLLKTRVREENFGFISNRFGNSWDFTSNYSSSEIG 336
                  VKC+ V +FLG SSVGFCCLL++R RE NF  +S RFG SWD++ +YS+S +G
Sbjct: 431 -------VKCKVVFDFLGSSSVGFCCLLESRFRERNFESVSKRFGYSWDYSCSYSNSGVG 490

Query: 337 RMWVMWKKSRFVLTPGEEEDQFVSRSLTDLLSGDCVEVLCVYAFNNNVERRLLWRRMGEI 396
           R+WVMWKK+RF  +    ++QFV  +LTDLLS  CVEV CVYA  +N+ERRLLWRR+ EI
Sbjct: 491 RIWVMWKKNRFSFSTRMVDEQFVIGTLTDLLSDVCVEVFCVYASYSNIERRLLWRRLVEI 550

Query: 397 SSRWTRPNDVIGDFNAIRLHSEAFGGSSVTGYIEEFDMVIREADLVEPSI 421
           +S W+ P  V+GDFN IR+HSEAFGGS   G +E+FD+ I +ADL+EPS+
Sbjct: 551 TSGWSSPGVVMGDFNVIRVHSEAFGGSPNQGEMEDFDLTILDADLIEPSV 580

BLAST of CSPI04G16910 vs. NCBI nr
Match: TYK28099.1 (uncharacterized protein E5676_scaffold1467G00020 [Cucumis melo var. makuwa])

HSP 1 Score: 395.2 bits (1014), Expect = 7.1e-106
Identity = 209/380 (55.00%), Postives = 258/380 (67.89%), Query Frame = 0

Query: 65  KHLSLDLATKERRKLSFARVCVELNVESPMLAEITVNLRRVDFIVTVNYEWKPRKCNLYR 124
           K +SLD ATK+RR+LS+ARVCVEL   S M AEITV+LR VDF V+VNYEWKPRKCNL  
Sbjct: 256 KPISLDFATKKRRRLSYARVCVELEGGSNMSAEITVSLRGVDFNVSVNYEWKPRKCNLCC 315

Query: 125 AFRHCSGKCPRIVENKVHHEEEVRK-------------------LEEGEINSSLIRPTK- 184
           AF H S KC R VE+K   EE V K                   +E+GEI SS  R    
Sbjct: 316 AFGHSSSKCSRSVESKTIQEEVVHKGDDVDFELCGEVVLESFKQVEKGEIRSSPNRHDSQ 375

Query: 185 -EKGVGNQDDFTLVIGKKWELVYVRYKGKSVDVTMPNSFGNLMELGKGDKWALSIVEGSA 244
            EKGVG  D+FTLV  KK ELV +R +GKS++V MPNSFG+L+E+G  DKWALSI+EGS+
Sbjct: 376 VEKGVGKSDEFTLVTRKKSELVSIRDRGKSMEVIMPNSFGSLLEVGDADKWALSIIEGSS 435

Query: 245 LPLQVDDTTMVLKGMRSDGNPAGTKASNSSHDYVVKCRAVLNFLGVSSVGFCCLLKTRVR 304
            PLQVD+ T VL G+ S                             S VGFCCLL+TRVR
Sbjct: 436 PPLQVDEGTDVLSGISS-----------------------------SIVGFCCLLETRVR 495

Query: 305 EENFGFISNRFGNSWDFTSNYSSSEIGRMWVMWKKSRFVLTPGEEEDQFVSRSLTDLLSG 364
           E NF  +S RF NSWD++ +YS+S +GR+WVMWKK+RF  +    ++QFV+ +LTDLLSG
Sbjct: 496 EGNFDSVSRRFSNSWDYSCSYSNSGVGRIWVMWKKNRFSFSTHVMDEQFVTGTLTDLLSG 555

Query: 365 DCVEVLCVYAFNNNVERRLLWRRMGEISSRWTRPNDVIGDFNAIRLHSEAFGGSSVTGYI 424
            CVEV CVYA N+N+ERRLLW R+ EI+S W+ P  V+ DFNAIR+HSEAF GS + G +
Sbjct: 556 VCVEVFCVYASNSNIERRLLWHRLVEITSAWSSPGVVMEDFNAIRVHSEAFRGSPIQGEM 606

BLAST of CSPI04G16910 vs. TAIR 10
Match: AT2G01050.1 (zinc ion binding;nucleic acid binding )

HSP 1 Score: 58.2 bits (139), Expect = 1.9e-08
Identity = 49/184 (26.63%), Postives = 76/184 (41.30%), Query Frame = 0

Query: 38  PKWIKLGRIPMELWINIRLDVVASAIGKHLSLDLATKERRKLSFARVCVELNVESPMLAE 97
           P W++L  IP   +    L  +A  +G+ L +D+ T    K  FARVC+E+N+  P+   
Sbjct: 165 PVWVRLSNIPYNYYHRCLLMEIARGLGRPLKVDMNTINFDKGRFARVCIEVNLAKPLKGT 224

Query: 98  ITVNLRRVDFIVTVNYEWKPRKCNLYRAFRHCSGKCPRIVENKVHHEEE-------VRKL 157
           + +N  R      V YE   + C+    + H    CPR V  KV    E       V   
Sbjct: 225 VLINGDR----YFVAYEGLSKICSSCGIYGHLVHSCPRNVVVKVSAGAETVTDRAVVPVG 284

Query: 158 EEGEINSSLIRPTKEKGVGNQDDFTLVIGKKW--ELVYVRYKGKSVDVTMPNSFGNLMEL 213
            EG+   ++++ T  +           +G         +R   K+  V + N FG L   
Sbjct: 285 MEGDDGFTVVQRTARRPAAPVQKMVFAVGASGGRSKQRLRELPKNQGVDLANRFGGLD-- 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7V5J27.9e-12756.50Non-LTR retroelement reverse transcriptase-like protein OS=Cucumis melo var. mak... [more]
A0A5A7U3C21.6e-11957.18Exo_endo_phos domain-containing protein/DUF4283 domain-containing protein OS=Cuc... [more]
A0A5A7V0841.8e-11855.61Exo_endo_phos domain-containing protein/DUF4283 domain-containing protein OS=Cuc... [more]
A0A5A7SPE53.4e-10655.00Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1... [more]
A0A5D3DXE43.4e-10655.00Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1... [more]
Match NameE-valueIdentityDescription
XP_004142722.24.3e-20490.02uncharacterized protein LOC101204295 [Cucumis sativus][more]
KAA0062888.11.6e-12656.50non-LTR retroelement reverse transcriptase-like protein [Cucumis melo var. makuw... [more]
KAA0049770.13.3e-11957.18hypothetical protein E6C27_scaffold76G001230 [Cucumis melo var. makuwa] >TYK1210... [more]
KAA0060724.13.6e-11855.61hypothetical protein E6C27_scaffold72G00110 [Cucumis melo var. makuwa] >TYK01466... [more]
TYK28099.17.1e-10655.00uncharacterized protein E5676_scaffold1467G00020 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT2G01050.11.9e-0826.63zinc ion binding;nucleic acid binding [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025558Domain of unknown function DUF4283PFAMPF14111DUF4283coord: 32..86
e-value: 4.9E-9
score: 35.9
IPR036691Endonuclease/exonuclease/phosphatase superfamilyGENE3D3.60.10.10Endonuclease/exonuclease/phosphatasecoord: 245..421
e-value: 8.2E-7
score: 31.0
NoneNo IPR availablePANTHERPTHR31286:SF49SUBFAMILY NOT NAMEDcoord: 35..138
IPR040256Uncharacterized protein At4g02000-likePANTHERPTHR31286GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1.8-LIKEcoord: 35..138

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G16910.1CSPI04G16910.1mRNA