CSPI04G12960 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G12960
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionAcetyl-coenzyme A synthetase
LocationChr4: 11195224 .. 11202594 (+)
RNA-Seq ExpressionCSPI04G12960
SyntenyCSPI04G12960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTCAAACTCTGCTTGAATTGTCCCCAATGGAGTTTTGATTTCGAAGGGAAAAACAAAATGGGAAAGAGCGTTTGGGAATTGGGTATTGAAGATTTAGTGAAAACAGGCTTAAATCTCCATGAAGCTGTGGAATTCAACAGGATTCTTCAGAATGTACTGTCCACAGCCCAAAAGCCTACTGAGGTTTGGCGGGAGCTCATGGCTCGAAGAGCCTTGAAACCCACACACCCATATGAGTTGCATCACCTTCTCTATTACTCTGTCTATGCTAATTGGAATATCTCTTCCAATGGACCTCCTCCATATTGGTTTCCTTCTATGTAATGTTTCTCATTTCATTCACCTAATTTTTATAGAGTTTCTGTGTCTTTGTAATGCCTATTGTCATATTTCTACTTGTAGTAATCAATCTAAACTGACAAACTTGGGAAAGATCATGGAGTTGCATGGTCCAAAGCTGTTGGGAGCTTCATATAAAGATCCTATCTCCAGTTTCAGCTTGTTTCAGAAACTCTCCTATCAGAGTCCTGAGGTTTGGATTCAATTCTGTATATATGGGGTTTTAATATGGAAATTGCATTCATTTTCGTGATTTATCTTGTGTTTCGGTGGTTATAGATTTACTGGTCAATTGTTCTTCACGAGCTTTCTGTTACTTTTCAAAAGCCGCCGAGGTGTATTTTAGATAGGACTGAGAAGTCAAATGTAAGATGGCTCCCTGACTCCATTTTGAACATTGCCGAGTGTTGTCTGCTTCCTTCAAGTCGTCCATTGAAAGATGATAATTCTTTGGCAATTGTGTGGAGGAATGAAGGCTGTGATAATTCAAATGTGAATCATATGACACTCAAACAACTTCGAGAGAAAGTAACGTATGCTCCTCTCTTTGACTACCGACGTTTCATCAAATTGATCATAAATATTGCTGATTATAATGAGAGGAGAGCTTTAGTATCAAGCAATTCTCTTGAAAAGCGGTTTATGATTATCTTCCTCTTTTTTAAAAATAAAAAAATAAAAAAATAGAAATTACTACGTACATCTTTTTACAAGGGCGTAAAAAAACCACTAACACTCCTCTGGGGAATCAACATGAAACCAACCTCGTGGACTGTTATTCTTTTTAATGAGATGTTGGCATGCTATGCAGGTTGGTGGCAAATGCACTTGATGCAACATTTTCAAAGGGCGATGCCATTGCTATTGACATGCCATTGACAGTTGATGCAGTTGTCATCTATTTAGCTATTGTTCTAGCAGGGCTTGTTGTTGTTTCTATTGCTGACAGCTTTGCTGCTAATGAAGTTGCAGTTCGTTTGCGTATATCAAAGGCAAAGGGAATATTTACTCAGGTAATTTTGTTAAAAACTTCAAAAAATAGAGTAGCTAAGAGACTCATGAGCTTTATATAGTAAATTTTTAGTGGGGAAAAGACCTCTTTTCAAGTAGTCATTTCTCAAAATAGATTATTGTCATTGTTGTTGATTTCCTCTCTGATGAATTTAACCGAGTCTTGATGTTTTCATGGCTTTGAAAACTGAAGATTGCATTTTGTTAGATTCTTTGTGCATTTGTTTTCAAAGTCAAGAATTAGAAATGTACAGAATTTCAAAGTCATTTCTCAAAATATGGATGCCATAAACAGGGACAATGTTTTACAACTACAGATTCTTATGCCATAAACCCTAAACTTTTACATTTGACTTTGTTTTCATTGCTTTTCAATGTTTAATTCTTATTTCATATTTCAATCAGGATTTCATATTAAGAGGAGGCAAGAAGTATCCTTTGTACAGGTAAAGTAACCAACACAAGACCCTGCGTTATCCTACAATGCCTTTGGTGAAAGAGAGTCGCGAGTTACAAGCGAACTTTTATATTCCTCTTTCCAGTGGCGAGGATCTTTATCTTTAACATACCAAGAACCATATTTCTAGATGGAATGATCTTAATAATTGGTAGCTTGATTAATCTGTCAACTTAGATTTAAGTCACCGTAGGCGTTAAAAGTGTTGGTATAATATTAAATTTATCTCAACCATTAGATTAAGTTTTTGGATAACTTGGTGATTTAACATGGTACAAGAGAAAAATGTCACGGGCTCAAACTCTTGTAATGTTATTTCCTCTTCAATTAACGTTGATTTACACTTGTTGGGTCTTCAACAAATTTATAAGGAGTATTAAAATGTTATTATAATATTAAATTTACCTTCACCCATGAGTTAACTTTTTTGGATCAATGGATGATTTAACACAATATTAATGCAGAAGGTTTTGTGATCAAACCAATGTTATTTCCTCCACAATTTATATTGATTCTTACTTATTGGGTCTTCTACAAATTTCTAATTCCAAAAGTGAAAGGGAATATCATAGTATTGATATAATATTAAACTTACCTTCTTCCATTAGCTTAATCTTTTGGGTCAATTAGTGACATGAAAATAGGTTTTTTGGAAGCCTACCATTTGACACATGCATTTGGTTCTCGAACAACTTATAGTTGCACTGCACTTTCTCACGGAAATTTGTTTCTTCTTGCAGTCGGGTTATACAAGGGGGGTCGTGTAAGGCTATTGTGATCCCCGCAACTGGGAACAGGCTGGATGTGGATTTGCGAGAACAAGATCTATCATGGGAAGAATTTCTTTCGACTGCAAAGGAATTTCCAAGGTAATGAAATTATAATGTTGACACATGTTGCTTTCTTATTACCTTCTTGTAAGTTCAATAAACTGTCCTACTTAAATTCAGCTTCCAATGAAGGTAACGTTCAAAAGATTATCAACAGTGCTGATTATGTTTTATTATGAATGAATCAGTAAAATTTGATCTGCATTGTTATACTGCAGATCAAATCGTTACTCTCCAGTATATCATTCAAGTGACGGCGTGACAAATATTCTCTTTTCATCTGGGACCACAGGTAAAAACAATTTTTAAGAACTACATCTGCATTGTTACTCTCTCCAAGCACCTTAAGAAGCACTGAAATTTTAATGTCTAGTGCTGGGTGTCTAGCGCATTCAGACACTCAAACACTTATGAGAACATGTATCGAACACTTGTTAGGGGAACGAATGTGTTAAAAATGCCTAGAACACTTGTTGTATCGACTAAAAAGACACATATATGACAACATTAATAAATTTTTAGAATGAAATACATTAAACCATTTTTAAGGTGCATTGTAAGACAATTTGGGGGAGAGGGGAATTGAAACCGGATCTCTTATCTTTGGCTACGTATAGATGTCAGTTGAGCTGAGCTCATGTTGACACATTAAACTAATTTTTTAAAGCAGATATATGCATTAATCCATTTACTTTGAATTTTCTTTGAGGATATATTTTAAAAAATAGTGTGTTGTTGTGTCTCTGTTTTTGTGTCATATCATATATGTGCTTCTTAGTCTATGATATATACCAAAGGAGAAAATTTTATTAATGTTTAAAAGTTGTGAATACAAGACCACAAATACTCTATTTATAATTGAAAAGCAATTAACAGCAATCCTAATTAATAAATGAAACTAATCTTAATCCTAATATATAAAAAAAACTAATCCTAATCCTAATAAATTAAGAATTTGACCATAATACTCTATTCCTACTTCATTGGTCTAGCTTCATATACAGTGGCAAATCCAAAAATTTTGTTCCGAGGGGCTTTGACTTCAATTTAGAAAGAGTATGGAGGCTAATAAGGTTCTTTTGCAATTATATTATGCAAACCTGGACTCGAACATCGCCATTCTAATCAATAATTTTAGTTTTTAGACCCAGAACTATTGATAAATCTTTGAGGATGTACTTCTTTCAAAGTGGGAACCAAAATAGTACTTTAACAATTTTTTAGTATAGAAATGTAGAGAAGCAACGCAAAAATATGGGGTGGGTAGGACCTTGAACCTGTGATGGCAACCACTATGGCAAGAACATTTTTTTGTTGAATAATCCATTTCACCAATTAATATTACTTCGCCGATAAATATTTGACGGGGGGCTCCTTAGCTGAAACCCCTACTGACCATATGTTTATTGATGAAGAAAATGTGTACAATATTTTGACATCCGCAGTTGCAAATTCTTATAAACTTGTGGCCCATATCAGGAGAGCCAAAAGCAATCCCATGGACACAACTTTCACCTATCCGCTGCGCTGCTGATTCCTGGGCTCACATGGATGTTCAGCCTGGTGATGTTTTCTGTTGGCCCACAAATTTAGGATGGGTGATGGGGCCAGTTTCAGTGTACTCATCCTTGTTAGCTGGTGCAACTCTTGCTCTCTATCATGGATCTCCTCTTGGCCATGGATTTGGAAAGTTCGTTCAGGTGAAAGTTTTGTTAGATGCCTATGAGCCCAACCATGGAATTTAATTGCTAGGACAACTAGTTTAAAATTTTAAACATTTCTTTCCTCTTCATTTGTTGTAGGATGCAGGAGTGACTTTATTGGGAACAGTTCCAAGCTTGGTAAAAACTTGGAAGGATACAAGGTGCATGGAAGGCTTAAATTGGACAAAAATAAGGTAATATTTTACAATTCTATATTGGAATGTTAAGTTATGATATTTAATGTCAGTTTAATGGTGTCAAAAAGAGCTTCGTTTCATTGACGTCAATGTATGTTTAAGAATTAAGAGGTCTATAGTTGAAATTTCTTCACTCCCGTATGTACTAAAATATATTTATTAGTTGAATGAACTTGGAAGTTGATATCTGCAGGCACTTTGCCTCTACTGGCGAAACGTCAAATGTCGATGATGATCTCTGGCTTTCCTCGAGATCATATTACAAACCTATATTTGAATTTTGTGGTGGCACAGAACTTGCCTCAAGCTACATCAACGGAACTCCACTACAACCCCAAGCTTTTGGGGCTTTTACCACAGCATCTATGACTACAGAATTTGTCATCCTTGATGAACATGGAATACCTTATGTGCGTACAAGAAATAGGCATTTATGTCATTTAGTAAATTTCTCAATGAAAAATCAAGTATTTTATTTTTTCTGTGCTTTTTTTTTTTTTATCAACAGCCAGAAGATCAACCTTGCATTGGTGAAATAGGGTTATTCCCTATATATTTGGGAGCTAGCAACGAATTGCTAAACGCTGATCATGATAAAGTTTACTTCAAGGGAATGCCAATATACAATGGAATGGTATAGTCAAGCTCAAGCACACCCTTCTTTTTGTTTATAGATTTCTTTTAATCTCTCAGGGACTTTTGGTACAAAACTTGGCTGTGTATTTATGCTCTGTTTCGGCATCCAAATGAATGTGGGTTATAGTATTGGTTGATCATGAATGCAACTTGCAAGTGAAATCTCATTTTTTAAAATAAGTAAGCAGGTATTTAGAATATCTTTTTACTTGATTCACTTCATTTCAGCAACTGAGAAGACATGGAGACATCATCAAAAGAACTGTTGGAGGCTACTTCGTTGTTCAAGGTAGGGCTGATGACACCATGAACCTTGGCGGCATCAAGGTACTACACACTGTTTTCTCTGATTTTCTGCTACTCCTAACATGAATGTCATTTTTCCAGTAAAATTCAATAGACTTGTGAAGTTGTTAGGAAACCTCCTAATTAGAATAATATCTAAAATATACTGAAATATAGAAGATGAAGAACCACAAGATAACCTAAGTATGAATGCATGAAACATCCCCTTCTTGAGTGTGTTGCAAGCCCTAGATCACTCCCCCTCCTGTGTATAACAAAATACACTAACTAACTCTATCTAATTACTCTTAAGCTCTTTACTAATATTCCTAACTAAGCACTTTATAGAAGTCAAGTACGCGGGACATTTACACGAGGTATAAATAAATTCAGAACAAAACTAAATTATATCTCAAATTCCCACTTCAAAGGTTAGAATCAAATAAGCGATCAATAAAGCGTAATAAAAAAGGAGAACAACACTGTAAATTAAACTGGCTCGCCTCGAACTCCGGAGTAAGTCCAGGCTTCTGACTTCTGCAACTACAGATTCGACTATGATAGAGATTGAAAAGACACCCAAATCCACGCTTACAAATCTCTCTTCCCAAATAGCCCAACAATAATTGTTTAAATTCAAGTACTTCACCCCAGTAACTTGAAATAAATAATAAGAGAAACCTTTTTTTAACCCAAAATAGAAAAATGCACACTGTGCTGTCACTCCAAAATACTGAAGAACAAAAACATTAATGCTCTCAGTTCTAATTCATCTTCGACGGCAAAGTGAATCACGGCCCAGACTTCTTTTTTTCCTTCAAAAGCCTCCTCCTTCTATAGAATAAACTCCACCGCCCATTTCTTTCTTTAATCATAACCACCTACAATTTTAGCCGATTAAAAAGCTTTCTTACCCAAGTTCATTAGAGGAAATGGGCTTTGAGAAAAAAATGGGTTAACATGAAAATAAGATGTTTCCACATGTATGTTGAGAAAGAAAAATTTTGTCCGCAAAAAAATTTGCTCAAGACTTGAGTAAATTGATTTTGAGCCAATAAGTTTGGGCTCCACATTCTTGACAAAGTAGGTAGCCAGTTCGAGCAACCTTATTACTTGCTTTTTCCTCCGTTAAAAGACAGACAAATTTCTTCCTGACTGTTAGATTATTTAACACCTACTTCTTTTACACACATGTAGACAAGTTCGGTAGAAATTGAACGGATATGCGACCATGTCGATGAGAGTATATTGGAAACAGCTGCAGTAAGTCTATCACCTATAGAAGGTGGTCCGGAACAACTAGTTATTCTGGTGGTACTAAGAAAGGAATATGAAAGGTCCCCACAAGAGCTGAAAGTCAAATTCTCAAAAGCAATTCAGAAAAATCTTAACCCTTTATTCAAGGTAGCATTTGACATTTTCTTTCCAATTTTTTCGGCAAACAAGGTCCTTAGATAATTTGAACTTTGAAGTGAATGAAACTGCAGGTTGGGTTTGTGAAAATTGTACCAAGCTTTCCACGAACTGCTTCTAACAAGTTACTGAGAAGAGTTTTGAGGAAACAGATGAAGGATGAACTTGCCCTTCGAAGTCAACTTTAGAGACCAAGACGAAATCTCTCTAAATTATTGAAATAAGATAGTTAATAGCAGTTCCATAAAGCTGCTTGAAAAGTAATTTAATATAGGTGTTTAATTGGAAGAAAATAAATCTAATTTATGACCTTTCAATAATTTATAGTTGCTTGTGTCTCGAAACATATGTTTAAATAGATAATTAGCACCTTTCATATAAAACTCTAGAAATCAAATCATTCGGATTCGCAAATCTAATTTCAAATCTTATATACAAGTTTCCAACATAGCAGGATTGTATTTTTCTATATGGAATCACCATCAAAGCTGCAGAAAC

mRNA sequence

GTTTCAAACTCTGCTTGAATTGTCCCCAATGGAGTTTTGATTTCGAAGGGAAAAACAAAATGGGAAAGAGCGTTTGGGAATTGGGTATTGAAGATTTAGTGAAAACAGGCTTAAATCTCCATGAAGCTGTGGAATTCAACAGGATTCTTCAGAATGTACTGTCCACAGCCCAAAAGCCTACTGAGGTTTGGCGGGAGCTCATGGCTCGAAGAGCCTTGAAACCCACACACCCATATGAGTTGCATCACCTTCTCTATTACTCTGTCTATGCTAATTGGAATATCTCTTCCAATGGACCTCCTCCATATTGGTTTCCTTCTATTAATCAATCTAAACTGACAAACTTGGGAAAGATCATGGAGTTGCATGGTCCAAAGCTGTTGGGAGCTTCATATAAAGATCCTATCTCCAGTTTCAGCTTGTTTCAGAAACTCTCCTATCAGAGTCCTGAGATTTACTGGTCAATTGTTCTTCACGAGCTTTCTGTTACTTTTCAAAAGCCGCCGAGGTGTATTTTAGATAGGACTGAGAAGTCAAATGTAAGATGGCTCCCTGACTCCATTTTGAACATTGCCGAGTGTTGTCTGCTTCCTTCAAGTCGTCCATTGAAAGATGATAATTCTTTGGCAATTGTGTGGAGGAATGAAGGCTGTGATAATTCAAATGTGAATCATATGACACTCAAACAACTTCGAGAGAAAGTAACGTTGGTGGCAAATGCACTTGATGCAACATTTTCAAAGGGCGATGCCATTGCTATTGACATGCCATTGACAGTTGATGCAGTTGTCATCTATTTAGCTATTGTTCTAGCAGGGCTTGTTGTTGTTTCTATTGCTGACAGCTTTGCTGCTAATGAAGTTGCAGTTCGTTTGCGTATATCAAAGGCAAAGGGAATATTTACTCAGGATTTCATATTAAGAGGAGGCAAGAAGTATCCTTTGTACAGTCGGGTTATACAAGGGGGGTCGTGTAAGGCTATTGTGATCCCCGCAACTGGGAACAGGCTGGATGTGGATTTGCGAGAACAAGATCTATCATGGGAAGAATTTCTTTCGACTGCAAAGGAATTTCCAAGATCAAATCGTTACTCTCCAGTATATCATTCAAGTGACGGCGTGACAAATATTCTCTTTTCATCTGGGACCACAGGAGAGCCAAAAGCAATCCCATGGACACAACTTTCACCTATCCGCTGCGCTGCTGATTCCTGGGCTCACATGGATGTTCAGCCTGGTGATGTTTTCTGTTGGCCCACAAATTTAGGATGGGTGATGGGGCCAGTTTCAGTGTACTCATCCTTGTTAGCTGGTGCAACTCTTGCTCTCTATCATGGATCTCCTCTTGGCCATGGATTTGGAAAGTTCGTTCAGGATGCAGGAGTGACTTTATTGGGAACAGTTCCAAGCTTGGTAAAAACTTGGAAGGATACAAGGTGCATGGAAGGCTTAAATTGGACAAAAATAAGGCACTTTGCCTCTACTGGCGAAACGTCAAATGTCGATGATGATCTCTGGCTTTCCTCGAGATCATATTACAAACCTATATTTGAATTTTGTGGTGGCACAGAACTTGCCTCAAGCTACATCAACGGAACTCCACTACAACCCCAAGCTTTTGGGGCTTTTACCACAGCATCTATGACTACAGAATTTGTCATCCTTGATGAACATGGAATACCTTATCCAGAAGATCAACCTTGCATTGGTGAAATAGGGTTATTCCCTATATATTTGGGAGCTAGCAACGAATTGCTAAACGCTGATCATGATAAAGTTTACTTCAAGGGAATGCCAATATACAATGGAATGCAACTGAGAAGACATGGAGACATCATCAAAAGAACTGTTGGAGGCTACTTCGTTGTTCAAGGTAGGGCTGATGACACCATGAACCTTGGCGGCATCAAGACAAGTTCGGTAGAAATTGAACGGATATGCGACCATGTCGATGAGAGTATATTGGAAACAGCTGCAGTAAGTCTATCACCTATAGAAGGTGGTCCGGAACAACTAGTTATTCTGGTGGTACTAAGAAAGGAATATGAAAGGTCCCCACAAGAGCTGAAAGTCAAATTCTCAAAAGCAATTCAGAAAAATCTTAACCCTTTATTCAAGGTTGGGTTTGTGAAAATTGTACCAAGCTTTCCACGAACTGCTTCTAACAAGTTACTGAGAAGAGTTTTGAGGAAACAGATGAAGGATGAACTTGCCCTTCGAAGTCAACTTTAGAGACCAAGACGAAATCTCTCTAAATTATTGAAATAAGATAGTTAATAGCAGTTCCATAAAGCTGCTTGAAAAGTAATTTAATATAGGTGTTTAATTGGAAGAAAATAAATCTAATTTATGACCTTTCAATAATTTATAGTTGCTTGTGTCTCGAAACATATGTTTAAATAGATAATTAGCACCTTTCATATAAAACTCTAGAAATCAAATCATTCGGATTCGCAAATCTAATTTCAAATCTTATATACAAGTTTCCAACATAGCAGGATTGTATTTTTCTATATGGAATCACCATCAAAGCTGCAGAAAC

Coding sequence (CDS)

ATGGGAAAGAGCGTTTGGGAATTGGGTATTGAAGATTTAGTGAAAACAGGCTTAAATCTCCATGAAGCTGTGGAATTCAACAGGATTCTTCAGAATGTACTGTCCACAGCCCAAAAGCCTACTGAGGTTTGGCGGGAGCTCATGGCTCGAAGAGCCTTGAAACCCACACACCCATATGAGTTGCATCACCTTCTCTATTACTCTGTCTATGCTAATTGGAATATCTCTTCCAATGGACCTCCTCCATATTGGTTTCCTTCTATTAATCAATCTAAACTGACAAACTTGGGAAAGATCATGGAGTTGCATGGTCCAAAGCTGTTGGGAGCTTCATATAAAGATCCTATCTCCAGTTTCAGCTTGTTTCAGAAACTCTCCTATCAGAGTCCTGAGATTTACTGGTCAATTGTTCTTCACGAGCTTTCTGTTACTTTTCAAAAGCCGCCGAGGTGTATTTTAGATAGGACTGAGAAGTCAAATGTAAGATGGCTCCCTGACTCCATTTTGAACATTGCCGAGTGTTGTCTGCTTCCTTCAAGTCGTCCATTGAAAGATGATAATTCTTTGGCAATTGTGTGGAGGAATGAAGGCTGTGATAATTCAAATGTGAATCATATGACACTCAAACAACTTCGAGAGAAAGTAACGTTGGTGGCAAATGCACTTGATGCAACATTTTCAAAGGGCGATGCCATTGCTATTGACATGCCATTGACAGTTGATGCAGTTGTCATCTATTTAGCTATTGTTCTAGCAGGGCTTGTTGTTGTTTCTATTGCTGACAGCTTTGCTGCTAATGAAGTTGCAGTTCGTTTGCGTATATCAAAGGCAAAGGGAATATTTACTCAGGATTTCATATTAAGAGGAGGCAAGAAGTATCCTTTGTACAGTCGGGTTATACAAGGGGGGTCGTGTAAGGCTATTGTGATCCCCGCAACTGGGAACAGGCTGGATGTGGATTTGCGAGAACAAGATCTATCATGGGAAGAATTTCTTTCGACTGCAAAGGAATTTCCAAGATCAAATCGTTACTCTCCAGTATATCATTCAAGTGACGGCGTGACAAATATTCTCTTTTCATCTGGGACCACAGGAGAGCCAAAAGCAATCCCATGGACACAACTTTCACCTATCCGCTGCGCTGCTGATTCCTGGGCTCACATGGATGTTCAGCCTGGTGATGTTTTCTGTTGGCCCACAAATTTAGGATGGGTGATGGGGCCAGTTTCAGTGTACTCATCCTTGTTAGCTGGTGCAACTCTTGCTCTCTATCATGGATCTCCTCTTGGCCATGGATTTGGAAAGTTCGTTCAGGATGCAGGAGTGACTTTATTGGGAACAGTTCCAAGCTTGGTAAAAACTTGGAAGGATACAAGGTGCATGGAAGGCTTAAATTGGACAAAAATAAGGCACTTTGCCTCTACTGGCGAAACGTCAAATGTCGATGATGATCTCTGGCTTTCCTCGAGATCATATTACAAACCTATATTTGAATTTTGTGGTGGCACAGAACTTGCCTCAAGCTACATCAACGGAACTCCACTACAACCCCAAGCTTTTGGGGCTTTTACCACAGCATCTATGACTACAGAATTTGTCATCCTTGATGAACATGGAATACCTTATCCAGAAGATCAACCTTGCATTGGTGAAATAGGGTTATTCCCTATATATTTGGGAGCTAGCAACGAATTGCTAAACGCTGATCATGATAAAGTTTACTTCAAGGGAATGCCAATATACAATGGAATGCAACTGAGAAGACATGGAGACATCATCAAAAGAACTGTTGGAGGCTACTTCGTTGTTCAAGGTAGGGCTGATGACACCATGAACCTTGGCGGCATCAAGACAAGTTCGGTAGAAATTGAACGGATATGCGACCATGTCGATGAGAGTATATTGGAAACAGCTGCAGTAAGTCTATCACCTATAGAAGGTGGTCCGGAACAACTAGTTATTCTGGTGGTACTAAGAAAGGAATATGAAAGGTCCCCACAAGAGCTGAAAGTCAAATTCTCAAAAGCAATTCAGAAAAATCTTAACCCTTTATTCAAGGTTGGGTTTGTGAAAATTGTACCAAGCTTTCCACGAACTGCTTCTAACAAGTTACTGAGAAGAGTTTTGAGGAAACAGATGAAGGATGAACTTGCCCTTCGAAGTCAACTTTAG

Protein sequence

MGKSVWELGIEDLVKTGLNLHEAVEFNRILQNVLSTAQKPTEVWRELMARRALKPTHPYELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISSFSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLPSSRPLKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPLTVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSRVIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNILFSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAGATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGETSNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEHGIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVLRKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELALRSQL*
Homology
BLAST of CSPI04G12960 vs. ExPASy Swiss-Prot
Match: M4IRL9 (Probable CoA ligase CCL12 OS=Humulus lupulus OX=3486 GN=CCL12 PE=2 SV=1)

HSP 1 Score: 1056.6 bits (2731), Expect = 1.3e-307
Identity = 505/721 (70.04%), Postives = 610/721 (84.60%), Query Frame = 0

Query: 7   ELGIEDLVKTGLNLHEAVEFNRILQNVLSTAQ--KPTEVWRELMARRALKPTHPYELHHL 66
           E+G+EDLVK GL+  EA  F R+L+++++ A+   P+EVWRE++ARR LKP+HP+ELH L
Sbjct: 3   EVGVEDLVKAGLSTEEAKYFQRVLEDIVNGAKGLDPSEVWREVVARRLLKPSHPHELHQL 62

Query: 67  LYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISSFSLFQK 126
           LY+SVYANW++S+ GPP YWFPS++QSK TNLG++ME +G ++LG  YKDPI+SFSLF+K
Sbjct: 63  LYHSVYANWDVSTRGPPLYWFPSLHQSKKTNLGRLMEKYGSEVLGPLYKDPITSFSLFRK 122

Query: 127 LSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKS--NVRWLPDSILNIAECCLLPSSRP 186
            S Q PE YWSIVL ELSV+FQ+ P+CILD  +KS  +   LP S+LNIAECCLLP+S P
Sbjct: 123 FSVQQPEAYWSIVLKELSVSFQEAPKCILDSADKSKRSGSLLPGSVLNIAECCLLPTSYP 182

Query: 187 LKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPLTVDA 246
           +K D+S AIVWR+EG D+S VN +TLK+LREKV LVANALDA FSKGDAIAIDMP+TV+A
Sbjct: 183 MKRDDSPAIVWRDEGYDDSEVNVLTLKELREKVMLVANALDAMFSKGDAIAIDMPMTVNA 242

Query: 247 VVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSRVIQG 306
           VVIYLAI+L+G VVVSIADSFAA E+A RLR+S AK IFTQDFILRGG+K+ LYSRV+  
Sbjct: 243 VVIYLAIILSGFVVVSIADSFAAKEIATRLRVSNAKAIFTQDFILRGGRKFSLYSRVVDA 302

Query: 307 GSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNILFSSG 366
              KAIV+P  G+ L + LREQD+SWE+F+S      RSN Y+P Y   D + NILFSSG
Sbjct: 303 APHKAIVLPVNGSNLGLQLREQDISWEKFISNVSNQARSNHYTPFYQPVDSLINILFSSG 362

Query: 367 TTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAGATLA 426
           TTGEPKAIPWTQLSP+RCAAD+WAH+D Q GDVFCWPTNLGWVMGPV ++SS L+GATLA
Sbjct: 363 TTGEPKAIPWTQLSPLRCAADTWAHIDAQVGDVFCWPTNLGWVMGPVLLFSSFLSGATLA 422

Query: 427 LYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGETSNVD 486
           LYHGSPLGHGFGKFVQD+GVT+LGTVPSLVK WK+T+CM+GL+WTKI+ FASTGE SNVD
Sbjct: 423 LYHGSPLGHGFGKFVQDSGVTILGTVPSLVKAWKNTQCMKGLDWTKIKVFASTGEASNVD 482

Query: 487 DDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEHGIPY 546
           DDLWLSS++YY+PI E CGGTELASSYI G+ +QPQAFGAF+TA+MT   VILDE+GIPY
Sbjct: 483 DDLWLSSQAYYQPIIECCGGTELASSYIQGSLVQPQAFGAFSTAAMTVGLVILDENGIPY 542

Query: 547 PEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVGGYFV 606
           P+ Q C GE+GLFP+YLGA++ LLNAD++KVYFKGMP+YNGM LRRHGDI+KRT GG+F+
Sbjct: 543 PDKQECTGEVGLFPLYLGATDRLLNADNEKVYFKGMPLYNGMSLRRHGDILKRTAGGFFI 602

Query: 607 VQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVLRKEY 666
           V GRADDTMNLGGIKTSS+EIER+C+  DES++ET AVS++P+ GGPEQLV+ VVL+  +
Sbjct: 603 VHGRADDTMNLGGIKTSSIEIERVCNRADESVMETTAVSVAPVSGGPEQLVMFVVLKSGH 662

Query: 667 ERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELALRSQ 724
               + LK KFSKAIQ NLNPLFKV FVKIV  FPRTASNKLLRRVLR QMK EL +RS+
Sbjct: 663 NNEAERLKKKFSKAIQSNLNPLFKVSFVKIVEEFPRTASNKLLRRVLRDQMKHELLVRSK 722

BLAST of CSPI04G12960 vs. ExPASy Swiss-Prot
Match: Q84P17 (Probable acyl-activating enzyme 18, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE18 PE=2 SV=1)

HSP 1 Score: 1012.7 bits (2617), Expect = 2.1e-294
Identity = 487/728 (66.90%), Postives = 594/728 (81.59%), Query Frame = 0

Query: 1   MGKSVWELGIEDLVKTGLNLHEAVEFNRILQNVLSTA--QKPTEVWRELMARRALKPTHP 60
           M KS+ EL  +D VK GL L +A EF++++ +V++ A    P + W+ L+    LKP HP
Sbjct: 1   MWKSIGELSCDDYVKAGLTLEDAKEFDKLVSDVITKAIETDPRDQWKALVDESVLKPWHP 60

Query: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           + LH LLYYSVY+NW+ S +GPP YWFPS++QSK TNLGK+ME HGP+LLG SYK+P+ S
Sbjct: 61  HPLHQLLYYSVYSNWDSSVHGPPLYWFPSLSQSKSTNLGKLMEYHGPRLLGPSYKNPLES 120

Query: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180
           F LF++ S + PE+YWS V+ ELS+ F  PPRCIL+++ K    WLPD++LNIAECCL+P
Sbjct: 121 FELFRRFSVEHPEVYWSFVIDELSLVFHTPPRCILNKS-KPEGTWLPDAVLNIAECCLMP 180

Query: 181 SSRPLKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SS P K+D+S+A+VWRNEG D+S VN MT+K+LRE+V LVANA+  +F KGD IAIDMP+
Sbjct: 181 SSHPKKEDDSVAVVWRNEGFDDSPVNRMTIKELREQVMLVANAISGSFEKGDTIAIDMPM 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAV+IYLAI+LAG +VVSIADSFAA E+A RL+ISKAKGIFTQD+ILRGG+++PLYSR
Sbjct: 241 TVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKISKAKGIFTQDYILRGGRRFPLYSR 300

Query: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPR-SNRYSPVYHSSDGVTNI 360
           V++    K IV+PA+G  L V LREQD+SW +FLS AK      N Y P+Y   + V NI
Sbjct: 301 VVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNAKPHSSGENYYRPIYLPVESVINI 360

Query: 361 LFSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLA 420
           LFSSGTTGEPKAIPWTQLSPIR A D WAH+DVQ G  +CWPTNLGWVMGP  ++S  L 
Sbjct: 361 LFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGHTYCWPTNLGWVMGPTLMFSCFLT 420

Query: 421 GATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGE 480
           GATLALY GSPLG GFGKFVQDAGVT+LGTVPSLVKTWK T CMEGLNWTKI+ FA+TGE
Sbjct: 421 GATLALYSGSPLGRGFGKFVQDAGVTVLGTVPSLVKTWKRTNCMEGLNWTKIKFFATTGE 480

Query: 481 TSNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDE 540
            SNVDD LWLSS++ YKP+ E CGGTELASSYI G+PLQPQAFGAF+T SMTT  +I DE
Sbjct: 481 ASNVDDVLWLSSKADYKPVIECCGGTELASSYIIGSPLQPQAFGAFSTPSMTTRIIIFDE 540

Query: 541 HGIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTV 600
           +G+PYP+DQPC GE+GLFP +LGA++ LLNA+HD+VYFKGMP+Y   +LRRHGDI+KRTV
Sbjct: 541 NGVPYPDDQPCTGEVGLFPQHLGATDRLLNANHDEVYFKGMPMYKETRLRRHGDIVKRTV 600

Query: 601 GGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVV 660
           GGY+ VQGRADDTMNLGGIKTSS+EIER+CD  DE I ETAAV+L+P  GGPE LVI  V
Sbjct: 601 GGYYNVQGRADDTMNLGGIKTSSIEIERVCDQADECISETAAVTLTPPNGGPELLVIFAV 660

Query: 661 LRKEY-ERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDE 720
           L++ + ++S +ELK+KFS+ IQK+LNPLFKV FVKIVP FPRTAS+KLLRRVLR Q+K E
Sbjct: 661 LKEGFKQQSGEELKMKFSRTIQKDLNPLFKVSFVKIVPEFPRTASSKLLRRVLRDQIKQE 720

Query: 721 -LALRSQL 724
            L+LRS++
Sbjct: 721 LLSLRSRI 727

BLAST of CSPI04G12960 vs. ExPASy Swiss-Prot
Match: F4KBF3 (Probable acyl-activating enzyme 17, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE17 PE=2 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 1.1e-202
Identity = 363/728 (49.86%), Postives = 490/728 (67.31%), Query Frame = 0

Query: 3   KSVWELGIEDLVKTGLNLHEAVEFNRILQNVLSTAQKPTEVWRELMARRALKPTHPYELH 62
           KS+  +   D+   G++   + +  R L++++  +  P E W ++ +RR L P  P+  H
Sbjct: 4   KSLNSITKSDIEALGISGDVSEKLLRDLEDIIHGSSTPPETWIQI-SRRILHPNLPFSFH 63

Query: 63  HLLYYSVYANWNISSNGPP-PYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISSFSL 122
            ++YY  Y ++     GP  P W P    + LTN+GK++E  G + LG +YK+P+SSFS 
Sbjct: 64  QMMYYGCYKDF-----GPDLPAWIPDPKVASLTNVGKLLEKRGKEFLGGNYKNPVSSFSS 123

Query: 123 FQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRT---EKSNVRWLPDSILNIAECCLLP 182
           FQ+ S  +PE+YW  VL EL++ F  PP+CIL++    +    +WLP + LN A  CL  
Sbjct: 124 FQEFSVSNPEVYWKTVLDELNILFSVPPKCILEKDTSGDNPGGKWLPGAYLNPARNCLTN 183

Query: 183 SSRPLKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDAT-FSKGDAIAIDMP 242
             +   DD  + I WR+EG D+  VN MTL +LR +V L A+AL A    +  AIA+DMP
Sbjct: 184 GFKRRLDD--IVIRWRDEGSDDLPVNTMTLLELRSQVWLAAHALSALGLEEESAIAVDMP 243

Query: 243 LTVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYS 302
           + V++V+IYLAIVLAG VVVSIADSF+  E++ RL+ISKAK IFTQD I+RG K  PLY 
Sbjct: 244 MNVESVIIYLAIVLAGHVVVSIADSFSPREISTRLKISKAKAIFTQDVIIRGDKSIPLYR 303

Query: 303 RVIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNI 362
           RV+   +  AIV+PA G+   + LRE+DLSW  FL  A+   R   Y      +   TNI
Sbjct: 304 RVVDAEAPLAIVVPARGSSCRMKLREKDLSWNNFLGNARNL-RGVEYVAAEKPAGAYTNI 363

Query: 363 LFSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLA 422
           LFSSGTTGEPKAIPWT +SP++ AAD+W H+DVQ GDV  WPTNLGW+MGP  VY+SL+ 
Sbjct: 364 LFSSGTTGEPKAIPWTNISPLKSAADAWCHLDVQRGDVVAWPTNLGWMMGPWLVYASLIN 423

Query: 423 GATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGE 482
           GA + LY+GSPLG  F KFVQDA V++LG +PS+V+TW+++    G +W++IR F STGE
Sbjct: 424 GACMGLYNGSPLGPTFAKFVQDAEVSVLGVIPSIVRTWQNSNSTSGYDWSRIRCFGSTGE 483

Query: 483 TSNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDE 542
            SN+D+ LWL  R++YKP+ E+CGGTE+  S+I+G+ LQPQ+  AF+TA+M  +  ILDE
Sbjct: 484 ASNIDEYLWLMGRAHYKPVIEYCGGTEIGGSFISGSLLQPQSLAAFSTAAMGCKLFILDE 543

Query: 543 HGIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTV 602
              P P     +GE+ L P   GAS+ LLN +H KVYF+GMP + G  LRRHGD+ +RT 
Sbjct: 544 DSNPIPPYAAGVGELALCPHMFGASSTLLNGNHFKVYFQGMPTFQGQILRRHGDLFERTS 603

Query: 603 GGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVV 662
            GY+   GRADDTMNLGGIK  S+EIER+C+ VD+S+LETAA+ + P  GGPEQLVI VV
Sbjct: 604 KGYYRAHGRADDTMNLGGIKVGSIEIERVCNSVDDSVLETAAIGVPPPSGGPEQLVIAVV 663

Query: 663 LRKEYERSP--QELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKD 722
            +     +P    LK  F+  IQK LNPLFKV  V  +PS PRTA+NK++RRVLR+Q+  
Sbjct: 664 FKSPEFSNPDLNLLKKSFNSEIQKKLNPLFKVSSVVTLPSLPRTATNKVMRRVLRQQL-T 721

Query: 723 ELALRSQL 724
           +  L S+L
Sbjct: 724 QTGLNSKL 721

BLAST of CSPI04G12960 vs. ExPASy Swiss-Prot
Match: Q88EH6 (Acetyl-coenzyme A synthetase 1 OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) OX=160488 GN=acsA1 PE=3 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 5.8e-42
Identity = 172/636 (27.04%), Postives = 286/636 (44.97%), Query Frame = 0

Query: 105 PKLLGASYKDPISSFSLFQKLSYQSPEIYWSIVLHELS--VTFQKPPRCILDRTEKSNVR 164
           P++   +  D  +  +++Q+ S  +P+ +W      +     F K  +   D     +++
Sbjct: 11  PEVAATTLTDEATYKAMYQQ-SVINPDGFWREQAQRIDWIKPFTKVKQTSFD-DHHVDIK 70

Query: 165 WLPDSILNIAECCLLPSSRPLKD-DNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANA 224
           W  D  LN++  CL    R L++  + LAI+W  EG D S   ++T ++L E+V   ANA
Sbjct: 71  WFADGTLNVSSNCL---DRHLEERGDQLAIIW--EGDDPSEHRNITYRELHEQVCKFANA 130

Query: 225 L-DATFSKGDAIAIDMPLTVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGI 284
           L      +GD + I MP+  +AVV  LA    G +   +   F+   +A R+   K+K +
Sbjct: 131 LRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVV 190

Query: 285 FTQDFILRGGKKYPLYSRV-------IQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLS 344
            T D  +RGG++ PL + V             K IV   TG  +    + +D+ +E+ + 
Sbjct: 191 ITADEGVRGGRRTPLKANVDLALTNPETSSVQKIIVCKRTGGDIAWH-QHRDIWYEDLMK 250

Query: 345 TAKEFPRSNRYSPVYHSSDGVTNILFSSGTTGEPKAIPWTQLSPIRCAADSWAHM-DVQP 404
            A     S+  +P    ++    IL++SG+TG+PK +  T    +  AA +   + D +P
Sbjct: 251 VA-----SSHCAPKEMGAEEALFILYTSGSTGKPKGVLHTTGGYLVYAALTHERVFDYRP 310

Query: 405 GDVFCWPTNLGWVMG-PVSVYSSLLAGATLALYHGSPLGHGF---GKFVQDAGVTLLGTV 464
           G+V+    ++GWV G    VY  L  GAT  L+ G P         K V    V +L T 
Sbjct: 311 GEVYWCTADVGWVTGHSYIVYGPLANGATTLLFEGVPNYPDITRVSKIVDKHKVNILYTA 370

Query: 465 PSLVKTW--KDTRCMEGLNWTKIRHFASTGETSNVDDDLWLSSRSYYK-------PIFEF 524
           P+ ++    +    +EG + + +R   S GE  N +   W     YYK       PI + 
Sbjct: 371 PTAIRAMMAEGQAAVEGADGSSLRLLGSVGEPINPEAWNW-----YYKTVGKERCPIVDT 430

Query: 525 CGGTE----LASSYINGTPLQPQAFGAFTTASMTTEFVILDEHGIPYPEDQPCIGEIGLF 584
              TE    L S     T L+P   G+ T         ++D  G     D    G + + 
Sbjct: 431 WWQTETGGILISPLPGATGLKP---GSATRPFFGVVPALVDNLGNLI--DGAAEGNLVIL 490

Query: 585 PIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVGGYFVVQGRADDTMNLGG 644
             + G S  L   DHD+        + GM     GD  +R   GY+ + GR DD +N+ G
Sbjct: 491 DSWPGQSRSLY-GDHDRFVDTYFKTFRGMYFT--GDGARRDEDGYYWITGRVDDVLNVSG 550

Query: 645 IKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVLRKEYERSPQELKVKFSK 704
            +  + EIE     V  S +  AAV   P +   + + + V L    E S ++L+++   
Sbjct: 551 HRMGTAEIESAM--VAHSKVAEAAVVGVPHDIKGQGIYVYVTLNAGIEAS-EQLRLELKN 610

Query: 705 AIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRK 712
            ++K + P+     ++  P  P+T S K++RR+LRK
Sbjct: 611 WVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617

BLAST of CSPI04G12960 vs. ExPASy Swiss-Prot
Match: Q4ZQG8 (Acetyl-coenzyme A synthetase OS=Pseudomonas syringae pv. syringae (strain B728a) OX=205918 GN=acsA PE=3 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 5.8e-42
Identity = 170/641 (26.52%), Postives = 284/641 (44.31%), Query Frame = 0

Query: 105 PKLLGASYKDPISSFSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRT---EKSNV 164
           P++   +  D  +  +++Q+ S  +P+ +W      L   + KP   +   +    + ++
Sbjct: 11  PEVAATTLTDEATYKAMYQQ-SVVNPDGFWREQAQRLD--WIKPFSVVKQTSFDDHRVDI 70

Query: 165 RWLPDSILNIAECCLLPSSRPLKD-DNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVAN 224
           +W  D  LN+A  CL    R L++  +S+AI+W  EG D S    +T ++L  +V   AN
Sbjct: 71  KWFADGTLNVAYNCL---DRHLEERGDSIAIIW--EGDDPSEHREITYRELHAEVCKFAN 130

Query: 225 AL-DATFSKGDAIAIDMPLTVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKG 284
           AL      +GD + I MP+  +AVV  LA    G +   +   F+   +A R+    +K 
Sbjct: 131 ALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCSSKV 190

Query: 285 IFTQDFILRGGKKYPLYSRVIQ-------GGSCKAIVIPATGNRLDVDLREQDLSWEEFL 344
           + T D  LRGGKK  L + V +           K IV   TG  ++ + R +D+ +   L
Sbjct: 191 VITADEGLRGGKKTALKANVDRALTNPETSSVQKVIVCKRTGGEIEWN-RHRDIWYHSLL 250

Query: 345 STAKEFPRSNRYSPVYHSSDGVTNILFSSGTTGEPKAIPWTQLSPIRCAADSWAHM-DVQ 404
             A     S+  +P    ++    IL++SG+TG+PK +  T    +  AA +   + D +
Sbjct: 251 EVA-----SSTCAPKEMGAEESLFILYTSGSTGKPKGVLHTTAGYLLYAALTHERVFDYR 310

Query: 405 PGDVFCWPTNLGWVMG-PVSVYSSLLAGATLALYHGSPLGHGF---GKFVQDAGVTLLGT 464
           PG+V+    ++GWV G    VY  L  GAT  L+ G P         K +    V +L T
Sbjct: 311 PGEVYWCTADVGWVTGHSYIVYGPLANGATTLLFEGVPNYPDITRVSKIIDKHKVNILYT 370

Query: 465 VPSLVKTW--KDTRCMEGLNWTKIRHFASTGETSNVDDDLWLSSRSYYK-------PIFE 524
            P+ ++    + T+ +EG + + +R   S GE  N +   W     YY        PI +
Sbjct: 371 APTAIRAMMAEGTKSVEGADGSSLRLLGSVGEPINPEAWAW-----YYNTVGKQNCPIVD 430

Query: 525 FCGGTE----LASSYINGTPLQPQAFGAFTTASMTTEFVILDEHGIPYPEDQPCIGEIGL 584
               TE    L S     T L+P   G+ T         ++D  G     +    G + +
Sbjct: 431 TWWQTETGGILISPLPGATALKP---GSATRPFFGVIPALVDNLGNLI--EGAAEGNLVI 490

Query: 585 FPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVGGYFVVQGRADDTMNLG 644
              + G S  L   DHD+        + GM     GD  +R   GYF + GR DD +N+ 
Sbjct: 491 LDSWPGQSRSLY-GDHDRFVDTYFKTFRGMYFT--GDGARRDEDGYFWITGRVDDVLNVS 550

Query: 645 GIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQL----VILVVLRKEYERSPQELK 704
           G +  + EIE        +++    V+ + + G P  L    + + V     E     L+
Sbjct: 551 GHRMGTAEIE-------SAMVAHPKVAEAAVVGVPHDLKGQGIYVYVTLNGGEEPSDALR 610

Query: 705 VKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRK 712
            +    ++K + P+    F++  P  P+T S K++RR+LRK
Sbjct: 611 TELRNWVRKEIGPIASPDFIQWAPGLPKTRSGKIMRRILRK 617

BLAST of CSPI04G12960 vs. ExPASy TrEMBL
Match: A0A0A0L1Q9 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G280430 PE=4 SV=1)

HSP 1 Score: 1452.6 bits (3759), Expect = 0.0e+00
Identity = 719/725 (99.17%), Postives = 720/725 (99.31%), Query Frame = 0

Query: 1   MGKSVWELGIEDLVKTGLNLHEAVEFNRILQNVLSTAQ--KPTEVWRELMARRALKPTHP 60
           MGKSVWEL IEDLVKTGLNLHEAVEFNRILQNVLSTAQ  KPTEVWRELMARRALKPTHP
Sbjct: 1   MGKSVWELDIEDLVKTGLNLHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60

Query: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180
           FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP
Sbjct: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180

Query: 181 SSRPLKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSRPLKDDNS AIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL
Sbjct: 181 SSRPLKDDNSSAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360
           VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL
Sbjct: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420
           FSSGTTGEPKAIPWTQ SPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG
Sbjct: 361 FSSGTTGEPKAIPWTQRSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLG+GFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET
Sbjct: 421 ATLALYHGSPLGYGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480

Query: 481 SNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEH 540
           SNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVG
Sbjct: 541 GIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVG 600

Query: 601 GYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660
           GYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL
Sbjct: 601 GYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660

Query: 661 RKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
           RKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA
Sbjct: 661 RKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720

Query: 721 LRSQL 724
           LRSQL
Sbjct: 721 LRSQL 725

BLAST of CSPI04G12960 vs. ExPASy TrEMBL
Match: A0A5A7TAS8 (Putative acyl-activating enzyme 18, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00370 PE=3 SV=1)

HSP 1 Score: 1349.3 bits (3491), Expect = 0.0e+00
Identity = 662/725 (91.31%), Postives = 695/725 (95.86%), Query Frame = 0

Query: 1   MGKSVWELGIEDLVKTGLNLHEAVEFNRILQNVLSTAQ--KPTEVWRELMARRALKPTHP 60
           MGKS+WELGIEDLV+TGLNLHEAVEFNRIL+NVLST Q  KPTEVWRELMARRALKPTHP
Sbjct: 1   MGKSIWELGIEDLVRTGLNLHEAVEFNRILKNVLSTTQRSKPTEVWRELMARRALKPTHP 60

Query: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           +ELH LLYYSVYANWNIS++GPPPYWFPS++QSKLTNLGKIMEL GPKLLGASYKDPISS
Sbjct: 61  FELHRLLYYSVYANWNISTSGPPPYWFPSLDQSKLTNLGKIMELQGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180
           FSLFQK S Q+PEIYWSIVL ELSVTFQK PRCILDRTEK N RWLPDS+LNIAECCL  
Sbjct: 121 FSLFQKFSIQNPEIYWSIVLQELSVTFQKAPRCILDRTEKPNGRWLPDSMLNIAECCLRS 180

Query: 181 SSRPLKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSR L+DDNSLAIVWR+EGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDA+AIDMPL
Sbjct: 181 SSRLLRDDNSLAIVWRDEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAVAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRL ISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLLISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360
           VI+GGSCKAIVIP TGNRL+VDLREQDLSWEEFLSTAKEFPRSN YSP+YHS D VTNIL
Sbjct: 301 VIEGGSCKAIVIPVTGNRLEVDLREQDLSWEEFLSTAKEFPRSNCYSPMYHSIDAVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420
           FSSGTTG+PKAIPWTQLSPIRCAADSWAHMDVQ GDVFCWPTNLGW MGPVSVYSSLL G
Sbjct: 361 FSSGTTGKPKAIPWTQLSPIRCAADSWAHMDVQAGDVFCWPTNLGWAMGPVSVYSSLLVG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLGHGFGKFVQDAGVT+LGT+PSLVKTWKDTRCMEGLNWTKIR+FASTGET
Sbjct: 421 ATLALYHGSPLGHGFGKFVQDAGVTILGTIPSLVKTWKDTRCMEGLNWTKIRYFASTGET 480

Query: 481 SNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEH 540
           SNVDDDLWLSSRSYYKP+FE CGGTEL SS+I G+P+QPQAFGAF+TASMTT FVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPVQPQAFGAFSTASMTTGFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIP+PEDQPCIGEIGLFPIYLGASNELLNADHD+VYFKGMPIYNGMQLRRHGDII+RTVG
Sbjct: 541 GIPFPEDQPCIGEIGLFPIYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIRRTVG 600

Query: 601 GYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660
           GY +VQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL
Sbjct: 601 GYLIVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660

Query: 661 RKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
           +K+Y +S QELK KFSKAIQ+NLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA
Sbjct: 661 KKQYVKSSQELKDKFSKAIQRNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720

Query: 721 LRSQL 724
           LRSQL
Sbjct: 721 LRSQL 725

BLAST of CSPI04G12960 vs. ExPASy TrEMBL
Match: A0A5D3DDN3 (Putative acyl-activating enzyme 18, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00570 PE=3 SV=1)

HSP 1 Score: 1349.0 bits (3490), Expect = 0.0e+00
Identity = 663/725 (91.45%), Postives = 693/725 (95.59%), Query Frame = 0

Query: 1   MGKSVWELGIEDLVKTGLNLHEAVEFNRILQNVLSTAQ--KPTEVWRELMARRALKPTHP 60
           MGKS+WELGIEDLVKTGLNLHEAVEFNRIL+NVLSTAQ  KP EVWRELMA+RALKPTHP
Sbjct: 1   MGKSIWELGIEDLVKTGLNLHEAVEFNRILKNVLSTAQRSKPAEVWRELMAKRALKPTHP 60

Query: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           +ELH LLYYSVYANWNIS+NGPPPYWFPS+NQSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  FELHRLLYYSVYANWNISTNGPPPYWFPSLNQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180
           FSLFQK S Q+PEIYWSIVL ELSVTFQK PRCILDRTEK N RWLPDS+LNIAECCL  
Sbjct: 121 FSLFQKFSIQNPEIYWSIVLQELSVTFQKAPRCILDRTEKPNGRWLPDSMLNIAECCLRS 180

Query: 181 SSRPLKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSR L+DDNSLAIVWR+EGCDNSNVNHMTLKQLR+KVTLVANALDATFSKGDA+AIDMPL
Sbjct: 181 SSRLLRDDNSLAIVWRDEGCDNSNVNHMTLKQLRDKVTLVANALDATFSKGDAVAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRL ISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLLISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360
           VI+GGSCKAIVIP TGNRL+VDLREQDLSWEEFLSTAKEFPRSN YSP+YHS D VTNIL
Sbjct: 301 VIEGGSCKAIVIPVTGNRLEVDLREQDLSWEEFLSTAKEFPRSNCYSPMYHSIDAVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420
           FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQ GDVFCWPTNLGW MGPVSVYSSLL G
Sbjct: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVFCWPTNLGWAMGPVSVYSSLLVG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLGHGFGKFVQDAGVT+LGTVPSLVKTWKDTRCMEGLNWTKIR+FASTGET
Sbjct: 421 ATLALYHGSPLGHGFGKFVQDAGVTILGTVPSLVKTWKDTRCMEGLNWTKIRYFASTGET 480

Query: 481 SNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEH 540
           SNVDDDLWLSSRSYYKP+FE CGGTEL SS+I G+PLQPQAFGAF+TASMTT FVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPLQPQAFGAFSTASMTTGFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIP+PEDQPCIGEIGLFPIYLGASNELLNADHD+VYFKGMPIYNGMQLRRHGDIIKRTVG
Sbjct: 541 GIPFPEDQPCIGEIGLFPIYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600

Query: 601 GYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660
           GY VVQGRADDTMNLGGIKTSSVE+ERICD VDESILETAAV++SPI+GGPEQLVILVVL
Sbjct: 601 GYLVVQGRADDTMNLGGIKTSSVELERICDLVDESILETAAVAVSPIQGGPEQLVILVVL 660

Query: 661 RKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
            + Y +SPQELKVKFSKAIQ NLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA
Sbjct: 661 SEGYRKSPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720

Query: 721 LRSQL 724
           L+S L
Sbjct: 721 LQSHL 725

BLAST of CSPI04G12960 vs. ExPASy TrEMBL
Match: A0A1S3BNK5 (probable acyl-activating enzyme 18, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103491592 PE=3 SV=1)

HSP 1 Score: 1349.0 bits (3490), Expect = 0.0e+00
Identity = 663/725 (91.45%), Postives = 693/725 (95.59%), Query Frame = 0

Query: 1   MGKSVWELGIEDLVKTGLNLHEAVEFNRILQNVLSTAQ--KPTEVWRELMARRALKPTHP 60
           MGKS+WELGIEDLVKTGLNLHEAVEFNRIL+NVLSTAQ  KP EVWRELMA+RALKPTHP
Sbjct: 1   MGKSIWELGIEDLVKTGLNLHEAVEFNRILKNVLSTAQRSKPAEVWRELMAKRALKPTHP 60

Query: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           +ELH LLYYSVYANWNIS+NGPPPYWFPS+NQSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  FELHRLLYYSVYANWNISTNGPPPYWFPSLNQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180
           FSLFQK S Q+PEIYWSIVL ELSVTFQK PRCILDRTEK N RWLPDS+LNIAECCL  
Sbjct: 121 FSLFQKFSIQNPEIYWSIVLQELSVTFQKAPRCILDRTEKPNGRWLPDSMLNIAECCLRS 180

Query: 181 SSRPLKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSR L+DDNSLAIVWR+EGCDNSNVNHMTLKQLR+KVTLVANALDATFSKGDA+AIDMPL
Sbjct: 181 SSRLLRDDNSLAIVWRDEGCDNSNVNHMTLKQLRDKVTLVANALDATFSKGDAVAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRL ISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLLISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360
           VI+GGSCKAIVIP TGNRL+VDLREQDLSWEEFLSTAKEFPRSN YSP+YHS D VTNIL
Sbjct: 301 VIEGGSCKAIVIPVTGNRLEVDLREQDLSWEEFLSTAKEFPRSNCYSPMYHSIDAVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420
           FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQ GDVFCWPTNLGW MGPVSVYSSLL G
Sbjct: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVFCWPTNLGWAMGPVSVYSSLLVG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLGHGFGKFVQDAGVT+LGTVPSLVKTWKDTRCMEGLNWTKIR+FASTGET
Sbjct: 421 ATLALYHGSPLGHGFGKFVQDAGVTILGTVPSLVKTWKDTRCMEGLNWTKIRYFASTGET 480

Query: 481 SNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEH 540
           SNVDDDLWLSSRSYYKP+FE CGGTEL SS+I G+PLQPQAFGAF+TASMTT FVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPLQPQAFGAFSTASMTTGFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIP+PEDQPCIGEIGLFPIYLGASNELLNADHD+VYFKGMPIYNGMQLRRHGDIIKRTVG
Sbjct: 541 GIPFPEDQPCIGEIGLFPIYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600

Query: 601 GYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660
           GY VVQGRADDTMNLGGIKTSSVE+ERICD VDESILETAAV++SPI+GGPEQLVILVVL
Sbjct: 601 GYLVVQGRADDTMNLGGIKTSSVELERICDLVDESILETAAVAVSPIQGGPEQLVILVVL 660

Query: 661 RKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
            + Y +SPQELKVKFSKAIQ NLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA
Sbjct: 661 SEGYRKSPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720

Query: 721 LRSQL 724
           L+S L
Sbjct: 721 LQSHL 725

BLAST of CSPI04G12960 vs. ExPASy TrEMBL
Match: A0A6J1DJZ2 (probable acyl-activating enzyme 18, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111021232 PE=4 SV=1)

HSP 1 Score: 1276.2 bits (3301), Expect = 0.0e+00
Identity = 623/725 (85.93%), Postives = 668/725 (92.14%), Query Frame = 0

Query: 1   MGKSVWELGIEDLVKTGLNLHEAVEFNRILQNVLSTAQK--PTEVWRELMARRALKPTHP 60
           MGK++WELG+ED VK GLNL EAVEF+RILQNV+   ++  P EVWRELMARR LKPTHP
Sbjct: 1   MGKNIWELGVEDTVKAGLNLEEAVEFDRILQNVVLVPKRSDPREVWRELMARRVLKPTHP 60

Query: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           Y LH L+YYSVYANW+ SSNGPPPYWFPS++QSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  YGLHQLVYYSVYANWDSSSNGPPPYWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180
           +SLFQK S Q PEIYWSIVL ELSVTF K PRCILD  EKS  RWLPDSILNIAECCLLP
Sbjct: 121 YSLFQKFSSQHPEIYWSIVLEELSVTFHKEPRCILDMAEKSKGRWLPDSILNIAECCLLP 180

Query: 181 SSRPLKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSRPL+DDNSLAIVWR+EG DNSNVN MTLK+LREKVTLVANALDA FSKGDAIAIDMP+
Sbjct: 181 SSRPLRDDNSLAIVWRDEGGDNSNVNRMTLKELREKVTLVANALDARFSKGDAIAIDMPM 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TV AV+IYLAIVLAG VVVSIADSF+ANEVAVRLRIS AKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVHAVIIYLAIVLAGFVVVSIADSFSANEVAVRLRISNAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360
           VI+GGSCKAIVIPATGN L+VDLREQDLSWEEFLSTAKE PRSN YS VYHS D +TNIL
Sbjct: 301 VIEGGSCKAIVIPATGNTLEVDLREQDLSWEEFLSTAKELPRSNCYSAVYHSIDAMTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420
           FSSGTTG+PKAIPWTQLSPIR  AD+WAH+D+  GD+ CWPTNLGWV+GP S+YS LL G
Sbjct: 361 FSSGTTGDPKAIPWTQLSPIRAVADAWAHIDIHVGDILCWPTNLGWVVGPTSLYSCLLTG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLG GFGKFV+DAGVTLLGTVPSLVKTWKDT+CMEGL+WTKIR+F STGET
Sbjct: 421 ATLALYHGSPLGRGFGKFVEDAGVTLLGTVPSLVKTWKDTQCMEGLDWTKIRYFTSTGET 480

Query: 481 SNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEH 540
           SNVDDDLWLSSRSYYKPI E CGGTELASSYI G+PLQPQAFGAF+TASMTT FVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPIIECCGGTELASSYIMGSPLQPQAFGAFSTASMTTGFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           G+PYPEDQPC+GE+GLFP+YLGAS+ LLNADHD+VYFKGMPIYN MQLRRHGDIIKRTVG
Sbjct: 541 GMPYPEDQPCVGELGLFPLYLGASDTLLNADHDEVYFKGMPIYNEMQLRRHGDIIKRTVG 600

Query: 601 GYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660
           GYFVVQGRADDTMNLGGIKTSSVEIERICDH +ESILETAAV +SPIEGGPEQLVILVVL
Sbjct: 601 GYFVVQGRADDTMNLGGIKTSSVEIERICDHAEESILETAAVGVSPIEGGPEQLVILVVL 660

Query: 661 RKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
           RK Y RSPQELK+KFSKAIQ  LNPLFKVGFVKIVP+FPRTASNKLLRRVLRKQMKDELA
Sbjct: 661 RKGYRRSPQELKIKFSKAIQSKLNPLFKVGFVKIVPNFPRTASNKLLRRVLRKQMKDELA 720

Query: 721 LRSQL 724
           +RSQL
Sbjct: 721 VRSQL 725

BLAST of CSPI04G12960 vs. NCBI nr
Match: XP_004142170.3 (probable acyl-activating enzyme 18, peroxisomal [Cucumis sativus])

HSP 1 Score: 1457.2 bits (3771), Expect = 0.0e+00
Identity = 721/725 (99.45%), Postives = 721/725 (99.45%), Query Frame = 0

Query: 1   MGKSVWELGIEDLVKTGLNLHEAVEFNRILQNVLSTAQ--KPTEVWRELMARRALKPTHP 60
           MGKSVWEL IEDLVKTGLNLHEAVEFNRILQNVLSTAQ  KPTEVWRELMARRALKPTHP
Sbjct: 1   MGKSVWELDIEDLVKTGLNLHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60

Query: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180
           FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP
Sbjct: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180

Query: 181 SSRPLKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSRPLKDDNS AIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL
Sbjct: 181 SSRPLKDDNSSAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360
           VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL
Sbjct: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420
           FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG
Sbjct: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET
Sbjct: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480

Query: 481 SNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEH 540
           SNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVG
Sbjct: 541 GIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVG 600

Query: 601 GYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660
           GYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL
Sbjct: 601 GYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660

Query: 661 RKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
           RKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA
Sbjct: 661 RKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720

Query: 721 LRSQL 724
           LRSQL
Sbjct: 721 LRSQL 725

BLAST of CSPI04G12960 vs. NCBI nr
Match: KAE8649475.1 (hypothetical protein Csa_018177 [Cucumis sativus])

HSP 1 Score: 1407.9 bits (3643), Expect = 0.0e+00
Identity = 691/725 (95.31%), Postives = 712/725 (98.21%), Query Frame = 0

Query: 1   MGKSVWELGIEDLVKTGLNLHEAVEFNRILQNVLSTAQ--KPTEVWRELMARRALKPTHP 60
           MGKSVWEL IEDLVKTGLNLHEAVEFNRILQNVLSTAQ  KPTEVWRELMARRALKPTHP
Sbjct: 1   MGKSVWELDIEDLVKTGLNLHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60

Query: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180
           FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP
Sbjct: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180

Query: 181 SSRPLKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSRPLKDDNS AIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL
Sbjct: 181 SSRPLKDDNSSAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360
           VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL
Sbjct: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420
           FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG
Sbjct: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLGHGFGKFVQDAGVT+LGTVPSLVKTWKDTRCMEGLNWTKIR+FASTGET
Sbjct: 421 ATLALYHGSPLGHGFGKFVQDAGVTMLGTVPSLVKTWKDTRCMEGLNWTKIRYFASTGET 480

Query: 481 SNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEH 540
           SNVDDDLWLSSRSYYKP+FE CGGTEL SS+I G+P+QPQAFGAF+TASMTT FVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPVQPQAFGAFSTASMTTGFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIP P+DQPCIGEIGLFP YLGAS+ELLNADHD+VYFKGMPIYNGMQLRRHGDIIKRTVG
Sbjct: 541 GIPSPDDQPCIGEIGLFPTYLGASSELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600

Query: 601 GYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660
           GY VVQGRADDTMNLGGIKTSSVE+ERICDHVDES+LETAAV++SPI+GGPEQLVILVVL
Sbjct: 601 GYLVVQGRADDTMNLGGIKTSSVELERICDHVDESLLETAAVAVSPIQGGPEQLVILVVL 660

Query: 661 RKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
           R+ Y++SPQELK+KFSKAIQ NLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDE+A
Sbjct: 661 REGYKKSPQELKIKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDEIA 720

Query: 721 LRSQL 724
           L+SQL
Sbjct: 721 LQSQL 725

BLAST of CSPI04G12960 vs. NCBI nr
Match: KAA0040068.1 (putative acyl-activating enzyme 18, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 1349.3 bits (3491), Expect = 0.0e+00
Identity = 662/725 (91.31%), Postives = 695/725 (95.86%), Query Frame = 0

Query: 1   MGKSVWELGIEDLVKTGLNLHEAVEFNRILQNVLSTAQ--KPTEVWRELMARRALKPTHP 60
           MGKS+WELGIEDLV+TGLNLHEAVEFNRIL+NVLST Q  KPTEVWRELMARRALKPTHP
Sbjct: 1   MGKSIWELGIEDLVRTGLNLHEAVEFNRILKNVLSTTQRSKPTEVWRELMARRALKPTHP 60

Query: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           +ELH LLYYSVYANWNIS++GPPPYWFPS++QSKLTNLGKIMEL GPKLLGASYKDPISS
Sbjct: 61  FELHRLLYYSVYANWNISTSGPPPYWFPSLDQSKLTNLGKIMELQGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180
           FSLFQK S Q+PEIYWSIVL ELSVTFQK PRCILDRTEK N RWLPDS+LNIAECCL  
Sbjct: 121 FSLFQKFSIQNPEIYWSIVLQELSVTFQKAPRCILDRTEKPNGRWLPDSMLNIAECCLRS 180

Query: 181 SSRPLKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSR L+DDNSLAIVWR+EGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDA+AIDMPL
Sbjct: 181 SSRLLRDDNSLAIVWRDEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAVAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRL ISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLLISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360
           VI+GGSCKAIVIP TGNRL+VDLREQDLSWEEFLSTAKEFPRSN YSP+YHS D VTNIL
Sbjct: 301 VIEGGSCKAIVIPVTGNRLEVDLREQDLSWEEFLSTAKEFPRSNCYSPMYHSIDAVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420
           FSSGTTG+PKAIPWTQLSPIRCAADSWAHMDVQ GDVFCWPTNLGW MGPVSVYSSLL G
Sbjct: 361 FSSGTTGKPKAIPWTQLSPIRCAADSWAHMDVQAGDVFCWPTNLGWAMGPVSVYSSLLVG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLGHGFGKFVQDAGVT+LGT+PSLVKTWKDTRCMEGLNWTKIR+FASTGET
Sbjct: 421 ATLALYHGSPLGHGFGKFVQDAGVTILGTIPSLVKTWKDTRCMEGLNWTKIRYFASTGET 480

Query: 481 SNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEH 540
           SNVDDDLWLSSRSYYKP+FE CGGTEL SS+I G+P+QPQAFGAF+TASMTT FVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPVQPQAFGAFSTASMTTGFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIP+PEDQPCIGEIGLFPIYLGASNELLNADHD+VYFKGMPIYNGMQLRRHGDII+RTVG
Sbjct: 541 GIPFPEDQPCIGEIGLFPIYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIRRTVG 600

Query: 601 GYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660
           GY +VQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL
Sbjct: 601 GYLIVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660

Query: 661 RKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
           +K+Y +S QELK KFSKAIQ+NLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA
Sbjct: 661 KKQYVKSSQELKDKFSKAIQRNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720

Query: 721 LRSQL 724
           LRSQL
Sbjct: 721 LRSQL 725

BLAST of CSPI04G12960 vs. NCBI nr
Match: XP_008449821.2 (PREDICTED: probable acyl-activating enzyme 18, peroxisomal [Cucumis melo] >KAA0040067.1 putative acyl-activating enzyme 18, peroxisomal [Cucumis melo var. makuwa] >TYK21692.1 putative acyl-activating enzyme 18, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 1349.0 bits (3490), Expect = 0.0e+00
Identity = 663/725 (91.45%), Postives = 693/725 (95.59%), Query Frame = 0

Query: 1   MGKSVWELGIEDLVKTGLNLHEAVEFNRILQNVLSTAQ--KPTEVWRELMARRALKPTHP 60
           MGKS+WELGIEDLVKTGLNLHEAVEFNRIL+NVLSTAQ  KP EVWRELMA+RALKPTHP
Sbjct: 1   MGKSIWELGIEDLVKTGLNLHEAVEFNRILKNVLSTAQRSKPAEVWRELMAKRALKPTHP 60

Query: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           +ELH LLYYSVYANWNIS+NGPPPYWFPS+NQSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  FELHRLLYYSVYANWNISTNGPPPYWFPSLNQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180
           FSLFQK S Q+PEIYWSIVL ELSVTFQK PRCILDRTEK N RWLPDS+LNIAECCL  
Sbjct: 121 FSLFQKFSIQNPEIYWSIVLQELSVTFQKAPRCILDRTEKPNGRWLPDSMLNIAECCLRS 180

Query: 181 SSRPLKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSR L+DDNSLAIVWR+EGCDNSNVNHMTLKQLR+KVTLVANALDATFSKGDA+AIDMPL
Sbjct: 181 SSRLLRDDNSLAIVWRDEGCDNSNVNHMTLKQLRDKVTLVANALDATFSKGDAVAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRL ISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLLISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360
           VI+GGSCKAIVIP TGNRL+VDLREQDLSWEEFLSTAKEFPRSN YSP+YHS D VTNIL
Sbjct: 301 VIEGGSCKAIVIPVTGNRLEVDLREQDLSWEEFLSTAKEFPRSNCYSPMYHSIDAVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420
           FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQ GDVFCWPTNLGW MGPVSVYSSLL G
Sbjct: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVFCWPTNLGWAMGPVSVYSSLLVG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLGHGFGKFVQDAGVT+LGTVPSLVKTWKDTRCMEGLNWTKIR+FASTGET
Sbjct: 421 ATLALYHGSPLGHGFGKFVQDAGVTILGTVPSLVKTWKDTRCMEGLNWTKIRYFASTGET 480

Query: 481 SNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEH 540
           SNVDDDLWLSSRSYYKP+FE CGGTEL SS+I G+PLQPQAFGAF+TASMTT FVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPLQPQAFGAFSTASMTTGFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIP+PEDQPCIGEIGLFPIYLGASNELLNADHD+VYFKGMPIYNGMQLRRHGDIIKRTVG
Sbjct: 541 GIPFPEDQPCIGEIGLFPIYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600

Query: 601 GYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660
           GY VVQGRADDTMNLGGIKTSSVE+ERICD VDESILETAAV++SPI+GGPEQLVILVVL
Sbjct: 601 GYLVVQGRADDTMNLGGIKTSSVELERICDLVDESILETAAVAVSPIQGGPEQLVILVVL 660

Query: 661 RKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
            + Y +SPQELKVKFSKAIQ NLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA
Sbjct: 661 SEGYRKSPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720

Query: 721 LRSQL 724
           L+S L
Sbjct: 721 LQSHL 725

BLAST of CSPI04G12960 vs. NCBI nr
Match: XP_031740080.1 (probable acyl-activating enzyme 18, peroxisomal [Cucumis sativus])

HSP 1 Score: 1344.7 bits (3479), Expect = 0.0e+00
Identity = 657/725 (90.62%), Postives = 694/725 (95.72%), Query Frame = 0

Query: 1   MGKSVWELGIEDLVKTGLNLHEAVEFNRILQNVLSTAQ--KPTEVWRELMARRALKPTHP 60
           MGKS+WELGIEDLVKTGL LHEAVEFNRILQNVLSTAQ  KPTEVWRELMARRALKPTHP
Sbjct: 24  MGKSIWELGIEDLVKTGLKLHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 83

Query: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           YELH LLYYSVYANWNISSNGPPPYWFPSI+QSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 84  YELHRLLYYSVYANWNISSNGPPPYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISS 143

Query: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180
           FSLFQK S Q+PEIYWSIVL ELS+TFQK PRCILDRTEK N RWLPDS+LNIAECCL  
Sbjct: 144 FSLFQKFSIQNPEIYWSIVLQELSITFQKAPRCILDRTEKPNGRWLPDSMLNIAECCLRS 203

Query: 181 SSRPLKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSR L+DDNSLAIVWR+EGCDNSNVNHMTLKQLREKVTLVANALDATFSKGD +AIDMPL
Sbjct: 204 SSRLLRDDNSLAIVWRDEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDVVAIDMPL 263

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRL ISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 264 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLLISKAKGIFTQDFILRGGKKYPLYSR 323

Query: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360
           VI+GGSCKAIVIPA+GNRL+VDLREQDLSWEEF+STAKEFPRSN YSP+YHS D +TNIL
Sbjct: 324 VIEGGSCKAIVIPASGNRLEVDLREQDLSWEEFISTAKEFPRSNCYSPMYHSIDAMTNIL 383

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420
           FSSGTTGEPKAIPWTQ SPIRCAADSWAHMDVQPGDVFCWPTNLGW MGPVSVYS+ L G
Sbjct: 384 FSSGTTGEPKAIPWTQRSPIRCAADSWAHMDVQPGDVFCWPTNLGWAMGPVSVYSAFLTG 443

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLG+GFGKFVQDAGVT+LGTVPSLVKTWKDTRCMEGLNWTKIR+FASTGET
Sbjct: 444 ATLALYHGSPLGYGFGKFVQDAGVTMLGTVPSLVKTWKDTRCMEGLNWTKIRYFASTGET 503

Query: 481 SNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEH 540
           SNVDDDLWLSSRSYYKP+FE CGGTEL SS+I G+P+QPQAFGAF+TASMTT FVILDEH
Sbjct: 504 SNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPVQPQAFGAFSTASMTTGFVILDEH 563

Query: 541 GIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIP P+DQPCIGEIGLFP YLGAS+ELLNADHD+VYFKGMPIYNGMQLRRHGDIIKRTVG
Sbjct: 564 GIPSPDDQPCIGEIGLFPTYLGASSELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 623

Query: 601 GYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660
           GY VVQGRADDTMNLGGIKTSSVE+ERICDHVDES+LETAAV++SPI+GGPEQLVILVVL
Sbjct: 624 GYLVVQGRADDTMNLGGIKTSSVELERICDHVDESLLETAAVAVSPIQGGPEQLVILVVL 683

Query: 661 RKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
           R+ Y++SPQELK+KFSKAIQ NLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDE+A
Sbjct: 684 REGYKKSPQELKIKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDEIA 743

Query: 721 LRSQL 724
           L+SQL
Sbjct: 744 LQSQL 748

BLAST of CSPI04G12960 vs. TAIR 10
Match: AT1G55320.1 (acyl-activating enzyme 18 )

HSP 1 Score: 1012.7 bits (2617), Expect = 1.5e-295
Identity = 487/728 (66.90%), Postives = 594/728 (81.59%), Query Frame = 0

Query: 1   MGKSVWELGIEDLVKTGLNLHEAVEFNRILQNVLSTA--QKPTEVWRELMARRALKPTHP 60
           M KS+ EL  +D VK GL L +A EF++++ +V++ A    P + W+ L+    LKP HP
Sbjct: 1   MWKSIGELSCDDYVKAGLTLEDAKEFDKLVSDVITKAIETDPRDQWKALVDESVLKPWHP 60

Query: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           + LH LLYYSVY+NW+ S +GPP YWFPS++QSK TNLGK+ME HGP+LLG SYK+P+ S
Sbjct: 61  HPLHQLLYYSVYSNWDSSVHGPPLYWFPSLSQSKSTNLGKLMEYHGPRLLGPSYKNPLES 120

Query: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180
           F LF++ S + PE+YWS V+ ELS+ F  PPRCIL+++ K    WLPD++LNIAECCL+P
Sbjct: 121 FELFRRFSVEHPEVYWSFVIDELSLVFHTPPRCILNKS-KPEGTWLPDAVLNIAECCLMP 180

Query: 181 SSRPLKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SS P K+D+S+A+VWRNEG D+S VN MT+K+LRE+V LVANA+  +F KGD IAIDMP+
Sbjct: 181 SSHPKKEDDSVAVVWRNEGFDDSPVNRMTIKELREQVMLVANAISGSFEKGDTIAIDMPM 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAV+IYLAI+LAG +VVSIADSFAA E+A RL+ISKAKGIFTQD+ILRGG+++PLYSR
Sbjct: 241 TVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKISKAKGIFTQDYILRGGRRFPLYSR 300

Query: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPR-SNRYSPVYHSSDGVTNI 360
           V++    K IV+PA+G  L V LREQD+SW +FLS AK      N Y P+Y   + V NI
Sbjct: 301 VVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNAKPHSSGENYYRPIYLPVESVINI 360

Query: 361 LFSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLA 420
           LFSSGTTGEPKAIPWTQLSPIR A D WAH+DVQ G  +CWPTNLGWVMGP  ++S  L 
Sbjct: 361 LFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGHTYCWPTNLGWVMGPTLMFSCFLT 420

Query: 421 GATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGE 480
           GATLALY GSPLG GFGKFVQDAGVT+LGTVPSLVKTWK T CMEGLNWTKI+ FA+TGE
Sbjct: 421 GATLALYSGSPLGRGFGKFVQDAGVTVLGTVPSLVKTWKRTNCMEGLNWTKIKFFATTGE 480

Query: 481 TSNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDE 540
            SNVDD LWLSS++ YKP+ E CGGTELASSYI G+PLQPQAFGAF+T SMTT  +I DE
Sbjct: 481 ASNVDDVLWLSSKADYKPVIECCGGTELASSYIIGSPLQPQAFGAFSTPSMTTRIIIFDE 540

Query: 541 HGIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTV 600
           +G+PYP+DQPC GE+GLFP +LGA++ LLNA+HD+VYFKGMP+Y   +LRRHGDI+KRTV
Sbjct: 541 NGVPYPDDQPCTGEVGLFPQHLGATDRLLNANHDEVYFKGMPMYKETRLRRHGDIVKRTV 600

Query: 601 GGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVV 660
           GGY+ VQGRADDTMNLGGIKTSS+EIER+CD  DE I ETAAV+L+P  GGPE LVI  V
Sbjct: 601 GGYYNVQGRADDTMNLGGIKTSSIEIERVCDQADECISETAAVTLTPPNGGPELLVIFAV 660

Query: 661 LRKEY-ERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDE 720
           L++ + ++S +ELK+KFS+ IQK+LNPLFKV FVKIVP FPRTAS+KLLRRVLR Q+K E
Sbjct: 661 LKEGFKQQSGEELKMKFSRTIQKDLNPLFKVSFVKIVPEFPRTASSKLLRRVLRDQIKQE 720

Query: 721 -LALRSQL 724
            L+LRS++
Sbjct: 721 LLSLRSRI 727

BLAST of CSPI04G12960 vs. TAIR 10
Match: AT1G55320.2 (acyl-activating enzyme 18 )

HSP 1 Score: 747.7 bits (1929), Expect = 9.0e-216
Identity = 364/510 (71.37%), Postives = 428/510 (83.92%), Query Frame = 0

Query: 217 LVANALDATFSKGDAIAIDMPLTVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISK 276
           LVANA+  +F KGD IAIDMP+TVDAV+IYLAI+LAG +VVSIADSFAA E+A RL+ISK
Sbjct: 2   LVANAISGSFEKGDTIAIDMPMTVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKISK 61

Query: 277 AKGIFTQDFILRGGKKYPLYSRVIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAK 336
           AKGIFTQD+ILRGG+++PLYSRV++    K IV+PA+G  L V LREQD+SW +FLS AK
Sbjct: 62  AKGIFTQDYILRGGRRFPLYSRVVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNAK 121

Query: 337 EFPR-SNRYSPVYHSSDGVTNILFSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDV 396
                 N Y P+Y   + V NILFSSGTTGEPKAIPWTQLSPIR A D WAH+DVQ G  
Sbjct: 122 PHSSGENYYRPIYLPVESVINILFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGHT 181

Query: 397 FCWPTNLGWVMGPVSVYSSLLAGATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTW 456
           +CWPTNLGWVMGP  ++S  L GATLALY GSPLG GFGKFVQDAGVT+LGTVPSLVKTW
Sbjct: 182 YCWPTNLGWVMGPTLMFSCFLTGATLALYSGSPLGRGFGKFVQDAGVTVLGTVPSLVKTW 241

Query: 457 KDTRCMEGLNWTKIRHFASTGETSNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPL 516
           K T CMEGLNWTKI+ FA+TGE SNVDD LWLSS++ YKP+ E CGGTELASSYI G+PL
Sbjct: 242 KRTNCMEGLNWTKIKFFATTGEASNVDDVLWLSSKADYKPVIECCGGTELASSYIIGSPL 301

Query: 517 QPQAFGAFTTASMTTEFVILDEHGIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYF 576
           QPQAFGAF+T SMTT  +I DE+G+PYP+DQPC GE+GLFP +LGA++ LLNA+HD+VYF
Sbjct: 302 QPQAFGAFSTPSMTTRIIIFDENGVPYPDDQPCTGEVGLFPQHLGATDRLLNANHDEVYF 361

Query: 577 KGMPIYNGMQLRRHGDIIKRTVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESIL 636
           KGMP+Y   +LRRHGDI+KRTVGGY+ VQGRADDTMNLGGIKTSS+EIER+CD  DE I 
Sbjct: 362 KGMPMYKETRLRRHGDIVKRTVGGYYNVQGRADDTMNLGGIKTSSIEIERVCDQADECIS 421

Query: 637 ETAAVSLSPIEGGPEQLVILVVLRKEY-ERSPQELKVKFSKAIQKNLNPLFKVGFVKIVP 696
           ETAAV+L+P  GGPE LVI  VL++ + ++S +ELK+KFS+ IQK+LNPLFKV FVKIVP
Sbjct: 422 ETAAVTLTPPNGGPELLVIFAVLKEGFKQQSGEELKMKFSRTIQKDLNPLFKVSFVKIVP 481

Query: 697 SFPRTASNKLLRRVLRKQMKDE-LALRSQL 724
            FPRTAS+KLLRRVLR Q+K E L+LRS++
Sbjct: 482 EFPRTASSKLLRRVLRDQIKQELLSLRSRI 511

BLAST of CSPI04G12960 vs. TAIR 10
Match: AT5G23050.1 (acyl-activating enzyme 17 )

HSP 1 Score: 708.0 bits (1826), Expect = 7.9e-204
Identity = 363/728 (49.86%), Postives = 490/728 (67.31%), Query Frame = 0

Query: 3   KSVWELGIEDLVKTGLNLHEAVEFNRILQNVLSTAQKPTEVWRELMARRALKPTHPYELH 62
           KS+  +   D+   G++   + +  R L++++  +  P E W ++ +RR L P  P+  H
Sbjct: 4   KSLNSITKSDIEALGISGDVSEKLLRDLEDIIHGSSTPPETWIQI-SRRILHPNLPFSFH 63

Query: 63  HLLYYSVYANWNISSNGPP-PYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISSFSL 122
            ++YY  Y ++     GP  P W P    + LTN+GK++E  G + LG +YK+P+SSFS 
Sbjct: 64  QMMYYGCYKDF-----GPDLPAWIPDPKVASLTNVGKLLEKRGKEFLGGNYKNPVSSFSS 123

Query: 123 FQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRT---EKSNVRWLPDSILNIAECCLLP 182
           FQ+ S  +PE+YW  VL EL++ F  PP+CIL++    +    +WLP + LN A  CL  
Sbjct: 124 FQEFSVSNPEVYWKTVLDELNILFSVPPKCILEKDTSGDNPGGKWLPGAYLNPARNCLTN 183

Query: 183 SSRPLKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDAT-FSKGDAIAIDMP 242
             +   DD  + I WR+EG D+  VN MTL +LR +V L A+AL A    +  AIA+DMP
Sbjct: 184 GFKRRLDD--IVIRWRDEGSDDLPVNTMTLLELRSQVWLAAHALSALGLEEESAIAVDMP 243

Query: 243 LTVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYS 302
           + V++V+IYLAIVLAG VVVSIADSF+  E++ RL+ISKAK IFTQD I+RG K  PLY 
Sbjct: 244 MNVESVIIYLAIVLAGHVVVSIADSFSPREISTRLKISKAKAIFTQDVIIRGDKSIPLYR 303

Query: 303 RVIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNI 362
           RV+   +  AIV+PA G+   + LRE+DLSW  FL  A+   R   Y      +   TNI
Sbjct: 304 RVVDAEAPLAIVVPARGSSCRMKLREKDLSWNNFLGNARNL-RGVEYVAAEKPAGAYTNI 363

Query: 363 LFSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLA 422
           LFSSGTTGEPKAIPWT +SP++ AAD+W H+DVQ GDV  WPTNLGW+MGP  VY+SL+ 
Sbjct: 364 LFSSGTTGEPKAIPWTNISPLKSAADAWCHLDVQRGDVVAWPTNLGWMMGPWLVYASLIN 423

Query: 423 GATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGE 482
           GA + LY+GSPLG  F KFVQDA V++LG +PS+V+TW+++    G +W++IR F STGE
Sbjct: 424 GACMGLYNGSPLGPTFAKFVQDAEVSVLGVIPSIVRTWQNSNSTSGYDWSRIRCFGSTGE 483

Query: 483 TSNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDE 542
            SN+D+ LWL  R++YKP+ E+CGGTE+  S+I+G+ LQPQ+  AF+TA+M  +  ILDE
Sbjct: 484 ASNIDEYLWLMGRAHYKPVIEYCGGTEIGGSFISGSLLQPQSLAAFSTAAMGCKLFILDE 543

Query: 543 HGIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTV 602
              P P     +GE+ L P   GAS+ LLN +H KVYF+GMP + G  LRRHGD+ +RT 
Sbjct: 544 DSNPIPPYAAGVGELALCPHMFGASSTLLNGNHFKVYFQGMPTFQGQILRRHGDLFERTS 603

Query: 603 GGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVV 662
            GY+   GRADDTMNLGGIK  S+EIER+C+ VD+S+LETAA+ + P  GGPEQLVI VV
Sbjct: 604 KGYYRAHGRADDTMNLGGIKVGSIEIERVCNSVDDSVLETAAIGVPPPSGGPEQLVIAVV 663

Query: 663 LRKEYERSP--QELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKD 722
            +     +P    LK  F+  IQK LNPLFKV  V  +PS PRTA+NK++RRVLR+Q+  
Sbjct: 664 FKSPEFSNPDLNLLKKSFNSEIQKKLNPLFKVSSVVTLPSLPRTATNKVMRRVLRQQL-T 721

Query: 723 ELALRSQL 724
           +  L S+L
Sbjct: 724 QTGLNSKL 721

BLAST of CSPI04G12960 vs. TAIR 10
Match: AT5G36880.1 (acetyl-CoA synthetase )

HSP 1 Score: 119.8 bits (299), Expect = 9.2e-27
Identity = 154/621 (24.80%), Postives = 254/621 (40.90%), Query Frame = 0

Query: 124 KLSYQSPEIYWSIVLHELSVTFQKPPRCILD----RTEKSNVRWLPDSILNIAECCLLPS 183
           K S   P  +WS +  E     +   +   +    R    ++ W    I NI   CL  +
Sbjct: 65  KRSMDDPAAFWSDIASEFYWKQKWGDQVFSENLDVRKGPISIEWFKGGITNICYNCLDKN 124

Query: 184 SRPLKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANAL-DATFSKGDAIAIDMPL 243
                 D + AI W  EG +      +T  +L ++V  +AN L D    KGDA+ I +P+
Sbjct: 125 VEAGLGDKT-AIHW--EGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPM 184

Query: 244 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 303
            ++  +  LA    G V   +   F+A+ +A R+   K   I T + + RG K   L + 
Sbjct: 185 LMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAI 244

Query: 304 V-----------IQGGSCKAIVIPATGNRLDVDLRE-QDLSWEEFLSTAKEFPRSNRYSP 363
           V           +  G C          R +   +  +D+ W++ +S   ++P S     
Sbjct: 245 VDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQNGRDVWWQDVIS---QYPTSCEVEW 304

Query: 364 VYHSSDGVTNILFSSGTTGEPKAIPWTQLSPIRCAADSWAH-MDVQPGDVFCWPTNLGWV 423
           V   ++    +L++SG+TG+PK +  T    +   A ++ +  D +  DV+    + GW+
Sbjct: 305 V--DAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWI 364

Query: 424 MGPVSV-YSSLLAGATLALYHGSPLGHGFGK---FVQDAGVTLLGTVPSLVKTW--KDTR 483
            G   V Y  +L GAT+ ++ G+P     G+    V    V++  T P+LV++    D +
Sbjct: 365 TGHSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDK 424

Query: 484 CMEGLNWTKIRHFASTGETSNVDDDLWL-----SSRSYYKPIFEFCGGTELASSYINGTP 543
            +   +   +R   S GE  N     W       SR    PI +    TE     I   P
Sbjct: 425 FVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRC---PISDTWWQTETGGFMITPLP 484

Query: 544 -LQPQAFGAFTTASMTTEFVILDEHGIPYPEDQPCIGEIGLFPIYLGASNELLNADHDK- 603
              PQ  G+ T      + VI+DE G     +  C G + +   + GA   L   DH++ 
Sbjct: 485 GAWPQKPGSATFPFFGVQPVIVDEKGNEI--EGECSGYLCVKGSWPGAFRTLF-GDHERY 544

Query: 604 --VYFKGMPIYNGMQLRRHGDIIKRTVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHV 663
              YFK    Y        GD   R   GY+ + GR DD +N+ G +  + E+E     +
Sbjct: 545 ETTYFKPFAGY-----YFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESAL-VL 604

Query: 664 DESILETAAVSLSPIEGGPEQLVILVVLRKEYERSPQELKVKFSKAIQKNLNPLFKVGFV 712
                E A V +     G  Q +   V   E     +EL+      ++  +        +
Sbjct: 605 HPQCAEAAVVGIEHEVKG--QGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRI 663

BLAST of CSPI04G12960 vs. TAIR 10
Match: AT5G36880.2 (acetyl-CoA synthetase )

HSP 1 Score: 119.8 bits (299), Expect = 9.2e-27
Identity = 154/621 (24.80%), Postives = 254/621 (40.90%), Query Frame = 0

Query: 124 KLSYQSPEIYWSIVLHELSVTFQKPPRCILD----RTEKSNVRWLPDSILNIAECCLLPS 183
           K S   P  +WS +  E     +   +   +    R    ++ W    I NI   CL  +
Sbjct: 115 KRSMDDPAAFWSDIASEFYWKQKWGDQVFSENLDVRKGPISIEWFKGGITNICYNCLDKN 174

Query: 184 SRPLKDDNSLAIVWRNEGCDNSNVNHMTLKQLREKVTLVANAL-DATFSKGDAIAIDMPL 243
                 D + AI W  EG +      +T  +L ++V  +AN L D    KGDA+ I +P+
Sbjct: 175 VEAGLGDKT-AIHW--EGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPM 234

Query: 244 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 303
            ++  +  LA    G V   +   F+A+ +A R+   K   I T + + RG K   L + 
Sbjct: 235 LMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAI 294

Query: 304 V-----------IQGGSCKAIVIPATGNRLDVDLRE-QDLSWEEFLSTAKEFPRSNRYSP 363
           V           +  G C          R +   +  +D+ W++ +S   ++P S     
Sbjct: 295 VDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQNGRDVWWQDVIS---QYPTSCEVEW 354

Query: 364 VYHSSDGVTNILFSSGTTGEPKAIPWTQLSPIRCAADSWAH-MDVQPGDVFCWPTNLGWV 423
           V   ++    +L++SG+TG+PK +  T    +   A ++ +  D +  DV+    + GW+
Sbjct: 355 V--DAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWI 414

Query: 424 MGPVSV-YSSLLAGATLALYHGSPLGHGFGK---FVQDAGVTLLGTVPSLVKTW--KDTR 483
            G   V Y  +L GAT+ ++ G+P     G+    V    V++  T P+LV++    D +
Sbjct: 415 TGHSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDK 474

Query: 484 CMEGLNWTKIRHFASTGETSNVDDDLWL-----SSRSYYKPIFEFCGGTELASSYINGTP 543
            +   +   +R   S GE  N     W       SR    PI +    TE     I   P
Sbjct: 475 FVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRC---PISDTWWQTETGGFMITPLP 534

Query: 544 -LQPQAFGAFTTASMTTEFVILDEHGIPYPEDQPCIGEIGLFPIYLGASNELLNADHDK- 603
              PQ  G+ T      + VI+DE G     +  C G + +   + GA   L   DH++ 
Sbjct: 535 GAWPQKPGSATFPFFGVQPVIVDEKGNEI--EGECSGYLCVKGSWPGAFRTLF-GDHERY 594

Query: 604 --VYFKGMPIYNGMQLRRHGDIIKRTVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHV 663
              YFK    Y        GD   R   GY+ + GR DD +N+ G +  + E+E     +
Sbjct: 595 ETTYFKPFAGY-----YFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESAL-VL 654

Query: 664 DESILETAAVSLSPIEGGPEQLVILVVLRKEYERSPQELKVKFSKAIQKNLNPLFKVGFV 712
                E A V +     G  Q +   V   E     +EL+      ++  +        +
Sbjct: 655 HPQCAEAAVVGIEHEVKG--QGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRI 713

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
M4IRL91.3e-30770.04Probable CoA ligase CCL12 OS=Humulus lupulus OX=3486 GN=CCL12 PE=2 SV=1[more]
Q84P172.1e-29466.90Probable acyl-activating enzyme 18, peroxisomal OS=Arabidopsis thaliana OX=3702 ... [more]
F4KBF31.1e-20249.86Probable acyl-activating enzyme 17, peroxisomal OS=Arabidopsis thaliana OX=3702 ... [more]
Q88EH65.8e-4227.04Acetyl-coenzyme A synthetase 1 OS=Pseudomonas putida (strain ATCC 47054 / DSM 61... [more]
Q4ZQG85.8e-4226.52Acetyl-coenzyme A synthetase OS=Pseudomonas syringae pv. syringae (strain B728a)... [more]
Match NameE-valueIdentityDescription
A0A0A0L1Q90.0e+0099.17AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G280430... [more]
A0A5A7TAS80.0e+0091.31Putative acyl-activating enzyme 18, peroxisomal OS=Cucumis melo var. makuwa OX=1... [more]
A0A5D3DDN30.0e+0091.45Putative acyl-activating enzyme 18, peroxisomal OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3BNK50.0e+0091.45probable acyl-activating enzyme 18, peroxisomal OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1DJZ20.0e+0085.93probable acyl-activating enzyme 18, peroxisomal OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
XP_004142170.30.0e+0099.45probable acyl-activating enzyme 18, peroxisomal [Cucumis sativus][more]
KAE8649475.10.0e+0095.31hypothetical protein Csa_018177 [Cucumis sativus][more]
KAA0040068.10.0e+0091.31putative acyl-activating enzyme 18, peroxisomal [Cucumis melo var. makuwa][more]
XP_008449821.20.0e+0091.45PREDICTED: probable acyl-activating enzyme 18, peroxisomal [Cucumis melo] >KAA00... [more]
XP_031740080.10.0e+0090.62probable acyl-activating enzyme 18, peroxisomal [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G55320.11.5e-29566.90acyl-activating enzyme 18 [more]
AT1G55320.29.0e-21671.37acyl-activating enzyme 18 [more]
AT5G23050.17.9e-20449.86acyl-activating enzyme 17 [more]
AT5G36880.19.2e-2724.80acetyl-CoA synthetase [more]
AT5G36880.29.2e-2724.80acetyl-CoA synthetase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 105..580
e-value: 3.1E-77
score: 262.4
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 191..552
e-value: 3.8E-34
score: 118.0
NoneNo IPR availablePANTHERPTHR44378:SF4ACYL-ACTIVATING ENZYME 18, PEROXISOMAL ISOFORM X1-RELATEDcoord: 1..584
NoneNo IPR availablePANTHERPTHR44378ACYL-ACTIVATING ENZYME 17, PEROXISOMAL-RELATEDcoord: 1..584
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 124..552
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 357..368

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G12960.1CSPI04G12960.1mRNA
CSPI04G12960.2CSPI04G12960.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane