CSPI04G11610 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G11610
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionBnaA09g07220D protein
LocationChr4: 9941503 .. 9942914 (-)
RNA-Seq ExpressionCSPI04G11610
SyntenyCSPI04G11610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGGTCATACCTTTTGCACTTCGGAAGTTCAAACAACAATACCACTTCTAAGGGAAGTATATGATGATACCATGACCAAAGTGTTATATGTTGGCCCTGATACTTGTTCGATGATTTCCAAATTACTCATAGTCGACGAAGATGATTATGAAGCTTGGGGTGTGGAACCATATGACTCTGATTCTAGTTACTTCCATTGCTGGGATCTCATCCATAAAGGCATCATCCGTGTTGCCGATGTTAAGTTTGATCTGCCTTATGAGAAAAATTCATTTTCTCATGTTATAATTTCAGATACTTTGGAATACTTCTCTTCACGATACCTTAACAGCACAATTTTTGAGTTGATGAGGGTGTCTAGAGAGGGTGTTATCATATTTGCTGGTAAACCCCTTATTTTAGTTTCGTTTTATAGATATCCAAAACACTTGGAAGTGAGAGTTCATTATAACATAATTTCCTTTTCTTTTAATCTCAAACTATACTATCCTTATCATTTTTCCTTTCTTTCAATGTTATGCAGGTCATCCGGATTATCCAGTTTCAGAATTTACCAGATACAAATTTGACCATGAGGTTAGAATTATGGAACTTGATGAGTGTGTTTGGAATACATCCGTAATTTATAAATGAGTACATTTATTTGTTCCTCTTATATTCTATTAGGTTCTCAAGTACAAGTATATAACACCAAACACAAAGATAAAACTGGATTGCACTATCAAAAGAAATTACAATATAACAATAGCCCTTGAAGAGGGCTATATCTCTCCAACAATCTACTAAAAGTAGATAATTTCAAATTACGACCTCCCCTCATGAACTCTAACTTTCTCTATTTACAACCAAGACCGCTAGCTAATGACCAATATATACCTTACCAATATCCTCCTAATATTTCCGATGTATCTCCCAATAATACTCTCATAGATCATAGATAGTCAGGAAACAATCCGGCGGTGTTCTATCACTGAATATCACAGTTTGTTCACTTATATTGTTGCATATTCTGAAACAAGATGGGATGACAAAGAAACTAAATAGAGAATGTAGAATTATATACTGCTTTTTGGGAGGCAATGTTTGCCTTTTCTAGAGTAACTTTTAAGCTTATGCCTAATATTTGATAATCAATCATTCAAACTATCCTCAAGTTAGGCAATTCTAATAAAAAATTACTAATTTACATGCATTGGAATTTAACCTACTGATTAGCAATGACCCATTTGGCTTTTGCTTTTGTTTGTGGATTTTGCAGGCCAAACTCCGAAGTCCTTCGTGGTGGAAAAGGTATTTAAGTCAAAAGAATTTGGAAGAGAATGAAGCTGCTAGAAAGAGGTTTAAAAAAATTTTAAGGGAAATTTCATATAAACCAGCATGCCAAGTCTTTTTTCTCAAGTCAGGATGA

mRNA sequence

ATGGCAGGTCATACCTTTTGCACTTCGGAAGTTCAAACAACAATACCACTTCTAAGGGAAGTATATGATGATACCATGACCAAAGTGTTATATGTTGGCCCTGATACTTGTTCGATGATTTCCAAATTACTCATAGTCGACGAAGATGATTATGAAGCTTGGGGTGTGGAACCATATGACTCTGATTCTAGTTACTTCCATTGCTGGGATCTCATCCATAAAGGCATCATCCGTGTTGCCGATGTTAAGTTTGATCTGCCTTATGAGAAAAATTCATTTTCTCATGTTATAATTTCAGATACTTTGGAATACTTCTCTTCACGATACCTTAACAGCACAATTTTTGAGTTGATGAGGGTGTCTAGAGAGGGTGTTATCATATTTGCTGGTCATCCGGATTATCCAGTTTCAGAATTTACCAGATACAAATTTGACCATGAGGCCAAACTCCGAAGTCCTTCGTGGTGGAAAAGGTATTTAAGTCAAAAGAATTTGGAAGAGAATGAAGCTGCTAGAAAGAGGTTTAAAAAAATTTTAAGGGAAATTTCATATAAACCAGCATGCCAAGTCTTTTTTCTCAAGTCAGGATGA

Coding sequence (CDS)

ATGGCAGGTCATACCTTTTGCACTTCGGAAGTTCAAACAACAATACCACTTCTAAGGGAAGTATATGATGATACCATGACCAAAGTGTTATATGTTGGCCCTGATACTTGTTCGATGATTTCCAAATTACTCATAGTCGACGAAGATGATTATGAAGCTTGGGGTGTGGAACCATATGACTCTGATTCTAGTTACTTCCATTGCTGGGATCTCATCCATAAAGGCATCATCCGTGTTGCCGATGTTAAGTTTGATCTGCCTTATGAGAAAAATTCATTTTCTCATGTTATAATTTCAGATACTTTGGAATACTTCTCTTCACGATACCTTAACAGCACAATTTTTGAGTTGATGAGGGTGTCTAGAGAGGGTGTTATCATATTTGCTGGTCATCCGGATTATCCAGTTTCAGAATTTACCAGATACAAATTTGACCATGAGGCCAAACTCCGAAGTCCTTCGTGGTGGAAAAGGTATTTAAGTCAAAAGAATTTGGAAGAGAATGAAGCTGCTAGAAAGAGGTTTAAAAAAATTTTAAGGGAAATTTCATATAAACCAGCATGCCAAGTCTTTTTTCTCAAGTCAGGATGA

Protein sequence

MAGHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYDSDSSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRVSREGVIIFAGHPDYPVSEFTRYKFDHEAKLRSPSWWKRYLSQKNLEENEAARKRFKKILREISYKPACQVFFLKSG*
Homology
BLAST of CSPI04G11610 vs. ExPASy Swiss-Prot
Match: Q0WPN7 (Probable pectin methylesterase CGR3 OS=Arabidopsis thaliana OX=3702 GN=CGR3 PE=2 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 4.7e-55
Identity = 105/195 (53.85%), Postives = 140/195 (71.79%), Query Frame = 0

Query: 1   MAGHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYD 60
           + G   CT+EVQ  IP+L+  Y D+M KVL+VGP+TCS++S LL  +E++ EAWGVEPYD
Sbjct: 63  ITGDYSCTAEVQRAIPILKSAYGDSMRKVLHVGPETCSVVSSLL--NEEETEAWGVEPYD 122

Query: 61  SDSSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRV 120
            + +  +C  L+HKG++RVAD+KF LPY   SFS VI+SD L+Y S RYLN T+ EL RV
Sbjct: 123 VEDADSNCKSLLHKGLVRVADIKFPLPYRSKSFSLVIVSDALDYLSPRYLNKTVPELARV 182

Query: 121 SREGVIIFAGHPDYPVSEFTRY-KFDHEAKLRSPSWWKRYLSQKNLEENEAARKRFKKIL 180
           + +GV++ AG+P    ++     KF   AK+RS SWW R+ SQ NLEENEAA K+F++  
Sbjct: 183 ASDGVVLLAGNPGQQKAKGGELSKFGRPAKMRSSSWWIRFFSQTNLEENEAASKKFEQAA 242

Query: 181 REISYKPACQVFFLK 195
            + SYKPACQVF LK
Sbjct: 243 SKSSYKPACQVFHLK 255

BLAST of CSPI04G11610 vs. ExPASy Swiss-Prot
Match: Q9M2Y6 (Probable pectin methylesterase CGR2 OS=Arabidopsis thaliana OX=3702 GN=CGR2 PE=2 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 1.7e-52
Identity = 101/195 (51.79%), Postives = 135/195 (69.23%), Query Frame = 0

Query: 1   MAGHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYD 60
           + G   CT+EVQ  IP+L++ Y D M KVL+VGPDTCS++S LL   E++ EAWGVEPYD
Sbjct: 66  VTGDYSCTAEVQRAIPVLKKAYGDGMRKVLHVGPDTCSVVSSLL--KEEETEAWGVEPYD 125

Query: 61  SDSSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRV 120
            + +  HC   + KG++RVAD+KF LPY   SFS VI+SD L+Y S +YLN T+ EL RV
Sbjct: 126 IEDADSHCKSFVSKGLVRVADIKFPLPYRAKSFSLVIVSDALDYLSPKYLNKTVPELARV 185

Query: 121 SREGVIIFAGHPDYPVSEFTRY-KFDHEAKLRSPSWWKRYLSQKNLEENEAARKRFKKIL 180
           + +GV++FAG P    ++     KF   AK+RS SWW R+  Q NLEEN+A  K+F++ +
Sbjct: 186 ASDGVVLFAGLPGQQRAKVAELSKFGRPAKMRSASWWNRFFVQTNLEENDAPSKKFEQAV 245

Query: 181 REISYKPACQVFFLK 195
            +  YKPACQVF LK
Sbjct: 246 SKGLYKPACQVFHLK 258

BLAST of CSPI04G11610 vs. ExPASy TrEMBL
Match: A0A1S3BYV5 (uncharacterized protein At3g49720-like OS=Cucumis melo OX=3656 GN=LOC103494900 PE=4 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.4e-99
Identity = 176/194 (90.72%), Postives = 187/194 (96.39%), Query Frame = 0

Query: 3   GHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYDSD 62
           GHTFCTSEVQTTIPLLREVYDD+MTKVLYVGPDTCSMISKLLI DEDDYEAWG+EPY SD
Sbjct: 81  GHTFCTSEVQTTIPLLREVYDDSMTKVLYVGPDTCSMISKLLIDDEDDYEAWGLEPYGSD 140

Query: 63  SSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRVSR 122
           SSYF+CWDLIHKGIIRVADVKF LPY +NSFSHVIISDTLEYFSSRYLNSTIFELMRVSR
Sbjct: 141 SSYFNCWDLIHKGIIRVADVKFALPYGENSFSHVIISDTLEYFSSRYLNSTIFELMRVSR 200

Query: 123 EGVIIFAGHPDYPVSEFTRYKFDHEAKLRSPSWWKRYLSQKNLEENEAARKRFKKILREI 182
           EGVIIFAG+PDYP+SEFTR+KFD +AKLRSPSWWKRYL+QK LEENE ARKRFKKILREI
Sbjct: 201 EGVIIFAGYPDYPISEFTRFKFDRQAKLRSPSWWKRYLNQKELEENEVARKRFKKILREI 260

Query: 183 SYKPACQVFFLKSG 197
           SYKPACQ++FLKSG
Sbjct: 261 SYKPACQIYFLKSG 274

BLAST of CSPI04G11610 vs. ExPASy TrEMBL
Match: A0A5D3E063 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00250 PE=4 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 1.4e-73
Identity = 134/150 (89.33%), Postives = 144/150 (96.00%), Query Frame = 0

Query: 1   MAGHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYD 60
           MAGHTFCTSEVQTTIPLLREVYDD+MTKVLYVGPDTCSMISKLLI DEDDYEAWG+EPY 
Sbjct: 1   MAGHTFCTSEVQTTIPLLREVYDDSMTKVLYVGPDTCSMISKLLIDDEDDYEAWGLEPYG 60

Query: 61  SDSSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRV 120
           SDSSYF+CWDLIHKGIIRVADVKF LPY +NSFSHVIISDTLEYFSSRYLNSTIFELMRV
Sbjct: 61  SDSSYFNCWDLIHKGIIRVADVKFALPYGENSFSHVIISDTLEYFSSRYLNSTIFELMRV 120

Query: 121 SREGVIIFAGHPDYPVSEFTRYKFDHEAKL 151
           SREGVIIFAG+PDYP+SEFTR+KFD + ++
Sbjct: 121 SREGVIIFAGYPDYPISEFTRFKFDRQVRI 150

BLAST of CSPI04G11610 vs. ExPASy TrEMBL
Match: A0A6J1D594 (uncharacterized protein At3g49720-like OS=Momordica charantia OX=3673 GN=LOC111017728 PE=4 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 8.3e-71
Identity = 131/193 (67.88%), Postives = 158/193 (81.87%), Query Frame = 0

Query: 3   GHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYDSD 62
           GH FCT EV+ T+PLLREVYDD M KVLYVGPDTCS++S  L  DE+DYEAWGV+P+  D
Sbjct: 80  GHGFCTPEVEITMPLLREVYDDIMNKVLYVGPDTCSLVSGFL--DEEDYEAWGVDPFGFD 139

Query: 63  SSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRVSR 122
           ++   C DL+ +GI+RVAD+KF LPY  +SFSHV++SDTLEY SSRYLNST+ EL RVS 
Sbjct: 140 ANDSDCRDLVQQGIVRVADIKFPLPYRDSSFSHVVVSDTLEYLSSRYLNSTLAELTRVSS 199

Query: 123 EGVIIFAGHPDYPVSEFTRYKFDHEAKLRSPSWWKRYLSQKNLEENEAARKRFKKILREI 182
           EGVII+AG+P +PVSEFTRY    +AK RSPSWW+RYL Q+ LEEN AA+KR KKIL++I
Sbjct: 200 EGVIIYAGYPGFPVSEFTRY--SRQAKFRSPSWWRRYLVQRKLEENVAAKKRLKKILKDI 259

Query: 183 SYKPACQVFFLKS 196
           SYKPACQ+  LKS
Sbjct: 260 SYKPACQILLLKS 268

BLAST of CSPI04G11610 vs. ExPASy TrEMBL
Match: A0A0A0L156 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G179070 PE=4 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 7.0e-70
Identity = 135/142 (95.07%), Postives = 135/142 (95.07%), Query Frame = 0

Query: 1   MAGHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYD 60
           MAGHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYD
Sbjct: 1   MAGHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYD 60

Query: 61  SDSSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRV 120
            DSSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRV
Sbjct: 61  FDSSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRV 120

Query: 121 SREGVIIFAGHPDYPVSEFTRY 143
           SREGVIIFAG P   VS F RY
Sbjct: 121 SREGVIIFAGKPLILVS-FYRY 141

BLAST of CSPI04G11610 vs. ExPASy TrEMBL
Match: A0A6J1K5Y9 (uncharacterized protein At3g49720-like OS=Cucurbita maxima OX=3661 GN=LOC111491988 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 4.3e-67
Identity = 121/193 (62.69%), Postives = 157/193 (81.35%), Query Frame = 0

Query: 3   GHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYDSD 62
           GHTFCTSEVQT I LL+EVYD  M KVLYVGPDT S++ +LL  D+DDY  WG++PYD+D
Sbjct: 74  GHTFCTSEVQTIITLLKEVYDYNMNKVLYVGPDTGSVMPELL-EDKDDYVDWGMDPYDTD 133

Query: 63  SSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRVSR 122
            +   CWDLI + I+ V+D+KF LPY + SFSHVI+SDTLEY SSRYLN TI E MRVSR
Sbjct: 134 GADSCCWDLIEREIVHVSDMKFPLPYREKSFSHVIVSDTLEYMSSRYLNETIPEFMRVSR 193

Query: 123 EGVIIFAGHPDYPVSEFTRYKFDHEAKLRSPSWWKRYLSQKNLEENEAARKRFKKILREI 182
           +G++IFAGHPD+PVS+F R +++ +AKLRSPSWWK YL+Q+  +E+ AA++R   +L++I
Sbjct: 194 DGIVIFAGHPDFPVSKFNRSRYNRQAKLRSPSWWKMYLAQRKFKEDAAAKERLNNMLKDI 253

Query: 183 SYKPACQVFFLKS 196
           S++PACQ+F LKS
Sbjct: 254 SFEPACQIFLLKS 265

BLAST of CSPI04G11610 vs. NCBI nr
Match: XP_011654354.1 (probable pectin methylesterase CGR3 [Cucumis sativus])

HSP 1 Score: 402.9 bits (1034), Expect = 1.6e-108
Identity = 193/194 (99.48%), Postives = 193/194 (99.48%), Query Frame = 0

Query: 3   GHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYDSD 62
           GHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYD D
Sbjct: 81  GHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYDFD 140

Query: 63  SSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRVSR 122
           SSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRVSR
Sbjct: 141 SSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRVSR 200

Query: 123 EGVIIFAGHPDYPVSEFTRYKFDHEAKLRSPSWWKRYLSQKNLEENEAARKRFKKILREI 182
           EGVIIFAGHPDYPVSEFTRYKFDHEAKLRSPSWWKRYLSQKNLEENEAARKRFKKILREI
Sbjct: 201 EGVIIFAGHPDYPVSEFTRYKFDHEAKLRSPSWWKRYLSQKNLEENEAARKRFKKILREI 260

Query: 183 SYKPACQVFFLKSG 197
           SYKPACQVFFLKSG
Sbjct: 261 SYKPACQVFFLKSG 274

BLAST of CSPI04G11610 vs. NCBI nr
Match: KAE8649381.1 (hypothetical protein Csa_011838 [Cucumis sativus])

HSP 1 Score: 402.9 bits (1034), Expect = 1.6e-108
Identity = 193/194 (99.48%), Postives = 193/194 (99.48%), Query Frame = 0

Query: 3   GHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYDSD 62
           GHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYD D
Sbjct: 9   GHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYDFD 68

Query: 63  SSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRVSR 122
           SSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRVSR
Sbjct: 69  SSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRVSR 128

Query: 123 EGVIIFAGHPDYPVSEFTRYKFDHEAKLRSPSWWKRYLSQKNLEENEAARKRFKKILREI 182
           EGVIIFAGHPDYPVSEFTRYKFDHEAKLRSPSWWKRYLSQKNLEENEAARKRFKKILREI
Sbjct: 129 EGVIIFAGHPDYPVSEFTRYKFDHEAKLRSPSWWKRYLSQKNLEENEAARKRFKKILREI 188

Query: 183 SYKPACQVFFLKSG 197
           SYKPACQVFFLKSG
Sbjct: 189 SYKPACQVFFLKSG 202

BLAST of CSPI04G11610 vs. NCBI nr
Match: XP_008454502.1 (PREDICTED: uncharacterized protein At3g49720-like [Cucumis melo])

HSP 1 Score: 372.1 bits (954), Expect = 3.0e-99
Identity = 176/194 (90.72%), Postives = 187/194 (96.39%), Query Frame = 0

Query: 3   GHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYDSD 62
           GHTFCTSEVQTTIPLLREVYDD+MTKVLYVGPDTCSMISKLLI DEDDYEAWG+EPY SD
Sbjct: 81  GHTFCTSEVQTTIPLLREVYDDSMTKVLYVGPDTCSMISKLLIDDEDDYEAWGLEPYGSD 140

Query: 63  SSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRVSR 122
           SSYF+CWDLIHKGIIRVADVKF LPY +NSFSHVIISDTLEYFSSRYLNSTIFELMRVSR
Sbjct: 141 SSYFNCWDLIHKGIIRVADVKFALPYGENSFSHVIISDTLEYFSSRYLNSTIFELMRVSR 200

Query: 123 EGVIIFAGHPDYPVSEFTRYKFDHEAKLRSPSWWKRYLSQKNLEENEAARKRFKKILREI 182
           EGVIIFAG+PDYP+SEFTR+KFD +AKLRSPSWWKRYL+QK LEENE ARKRFKKILREI
Sbjct: 201 EGVIIFAGYPDYPISEFTRFKFDRQAKLRSPSWWKRYLNQKELEENEVARKRFKKILREI 260

Query: 183 SYKPACQVFFLKSG 197
           SYKPACQ++FLKSG
Sbjct: 261 SYKPACQIYFLKSG 274

BLAST of CSPI04G11610 vs. NCBI nr
Match: XP_038905892.1 (probable pectin methylesterase CGR3 [Benincasa hispida])

HSP 1 Score: 340.9 bits (873), Expect = 7.4e-90
Identity = 162/194 (83.51%), Postives = 180/194 (92.78%), Query Frame = 0

Query: 3   GHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYDSD 62
           G TFC+ EVQT IPLLREVYDD+MTKVLYVGPDTCSM+S+LLI DE++YEAWGVEPY SD
Sbjct: 79  GQTFCSLEVQTMIPLLREVYDDSMTKVLYVGPDTCSMMSELLI-DEEEYEAWGVEPYGSD 138

Query: 63  SSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRVSR 122
            S F+CWDLIHKGIIRVAD+KF LPY +NSFSHVIISDTLEYFS RYLNSTI ELMRVSR
Sbjct: 139 GSDFYCWDLIHKGIIRVADMKFSLPYRENSFSHVIISDTLEYFSPRYLNSTIPELMRVSR 198

Query: 123 EGVIIFAGHPDYPVSEFTRYKFDHEAKLRSPSWWKRYLSQKNLEENEAARKRFKKILREI 182
           +GVIIFAG+PDYP++EFTRYKF+ EAKLRSPSWWKRYL Q+ LEENEAA+KRFKKILR+I
Sbjct: 199 DGVIIFAGYPDYPITEFTRYKFNREAKLRSPSWWKRYLVQRKLEENEAAKKRFKKILRDI 258

Query: 183 SYKPACQVFFLKSG 197
           SYKPACQ+FFLKSG
Sbjct: 259 SYKPACQIFFLKSG 271

BLAST of CSPI04G11610 vs. NCBI nr
Match: TYK29433.1 (uncharacterized protein E5676_scaffold655G00250 [Cucumis melo var. makuwa])

HSP 1 Score: 285.8 bits (730), Expect = 2.8e-73
Identity = 134/150 (89.33%), Postives = 144/150 (96.00%), Query Frame = 0

Query: 1   MAGHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYD 60
           MAGHTFCTSEVQTTIPLLREVYDD+MTKVLYVGPDTCSMISKLLI DEDDYEAWG+EPY 
Sbjct: 1   MAGHTFCTSEVQTTIPLLREVYDDSMTKVLYVGPDTCSMISKLLIDDEDDYEAWGLEPYG 60

Query: 61  SDSSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRV 120
           SDSSYF+CWDLIHKGIIRVADVKF LPY +NSFSHVIISDTLEYFSSRYLNSTIFELMRV
Sbjct: 61  SDSSYFNCWDLIHKGIIRVADVKFALPYGENSFSHVIISDTLEYFSSRYLNSTIFELMRV 120

Query: 121 SREGVIIFAGHPDYPVSEFTRYKFDHEAKL 151
           SREGVIIFAG+PDYP+SEFTR+KFD + ++
Sbjct: 121 SREGVIIFAGYPDYPISEFTRFKFDRQVRI 150

BLAST of CSPI04G11610 vs. TAIR 10
Match: AT5G65810.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G49720.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 215.7 bits (548), Expect = 3.3e-56
Identity = 105/195 (53.85%), Postives = 140/195 (71.79%), Query Frame = 0

Query: 1   MAGHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYD 60
           + G   CT+EVQ  IP+L+  Y D+M KVL+VGP+TCS++S LL  +E++ EAWGVEPYD
Sbjct: 63  ITGDYSCTAEVQRAIPILKSAYGDSMRKVLHVGPETCSVVSSLL--NEEETEAWGVEPYD 122

Query: 61  SDSSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRV 120
            + +  +C  L+HKG++RVAD+KF LPY   SFS VI+SD L+Y S RYLN T+ EL RV
Sbjct: 123 VEDADSNCKSLLHKGLVRVADIKFPLPYRSKSFSLVIVSDALDYLSPRYLNKTVPELARV 182

Query: 121 SREGVIIFAGHPDYPVSEFTRY-KFDHEAKLRSPSWWKRYLSQKNLEENEAARKRFKKIL 180
           + +GV++ AG+P    ++     KF   AK+RS SWW R+ SQ NLEENEAA K+F++  
Sbjct: 183 ASDGVVLLAGNPGQQKAKGGELSKFGRPAKMRSSSWWIRFFSQTNLEENEAASKKFEQAA 242

Query: 181 REISYKPACQVFFLK 195
            + SYKPACQVF LK
Sbjct: 243 SKSSYKPACQVFHLK 255

BLAST of CSPI04G11610 vs. TAIR 10
Match: AT3G49720.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast thylakoid membrane, Golgi apparatus, plasma membrane, membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G65810.1); Has 64 Blast hits to 64 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 64; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 207.2 bits (526), Expect = 1.2e-53
Identity = 101/195 (51.79%), Postives = 135/195 (69.23%), Query Frame = 0

Query: 1   MAGHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYD 60
           + G   CT+EVQ  IP+L++ Y D M KVL+VGPDTCS++S LL   E++ EAWGVEPYD
Sbjct: 66  VTGDYSCTAEVQRAIPVLKKAYGDGMRKVLHVGPDTCSVVSSLL--KEEETEAWGVEPYD 125

Query: 61  SDSSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRV 120
            + +  HC   + KG++RVAD+KF LPY   SFS VI+SD L+Y S +YLN T+ EL RV
Sbjct: 126 IEDADSHCKSFVSKGLVRVADIKFPLPYRAKSFSLVIVSDALDYLSPKYLNKTVPELARV 185

Query: 121 SREGVIIFAGHPDYPVSEFTRY-KFDHEAKLRSPSWWKRYLSQKNLEENEAARKRFKKIL 180
           + +GV++FAG P    ++     KF   AK+RS SWW R+  Q NLEEN+A  K+F++ +
Sbjct: 186 ASDGVVLFAGLPGQQRAKVAELSKFGRPAKMRSASWWNRFFVQTNLEENDAPSKKFEQAV 245

Query: 181 REISYKPACQVFFLK 195
            +  YKPACQVF LK
Sbjct: 246 SKGLYKPACQVFHLK 258

BLAST of CSPI04G11610 vs. TAIR 10
Match: AT3G49720.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast thylakoid membrane, Golgi apparatus, plasma membrane, membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G65810.1); Has 64 Blast hits to 64 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 64; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 207.2 bits (526), Expect = 1.2e-53
Identity = 101/195 (51.79%), Postives = 135/195 (69.23%), Query Frame = 0

Query: 1   MAGHTFCTSEVQTTIPLLREVYDDTMTKVLYVGPDTCSMISKLLIVDEDDYEAWGVEPYD 60
           + G   CT+EVQ  IP+L++ Y D M KVL+VGPDTCS++S LL   E++ EAWGVEPYD
Sbjct: 66  VTGDYSCTAEVQRAIPVLKKAYGDGMRKVLHVGPDTCSVVSSLL--KEEETEAWGVEPYD 125

Query: 61  SDSSYFHCWDLIHKGIIRVADVKFDLPYEKNSFSHVIISDTLEYFSSRYLNSTIFELMRV 120
            + +  HC   + KG++RVAD+KF LPY   SFS VI+SD L+Y S +YLN T+ EL RV
Sbjct: 126 IEDADSHCKSFVSKGLVRVADIKFPLPYRAKSFSLVIVSDALDYLSPKYLNKTVPELARV 185

Query: 121 SREGVIIFAGHPDYPVSEFTRY-KFDHEAKLRSPSWWKRYLSQKNLEENEAARKRFKKIL 180
           + +GV++FAG P    ++     KF   AK+RS SWW R+  Q NLEEN+A  K+F++ +
Sbjct: 186 ASDGVVLFAGLPGQQRAKVAELSKFGRPAKMRSASWWNRFFVQTNLEENDAPSKKFEQAV 245

Query: 181 REISYKPACQVFFLK 195
            +  YKPACQVF LK
Sbjct: 246 SKGLYKPACQVFHLK 258

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0WPN74.7e-5553.85Probable pectin methylesterase CGR3 OS=Arabidopsis thaliana OX=3702 GN=CGR3 PE=2... [more]
Q9M2Y61.7e-5251.79Probable pectin methylesterase CGR2 OS=Arabidopsis thaliana OX=3702 GN=CGR2 PE=2... [more]
Match NameE-valueIdentityDescription
A0A1S3BYV51.4e-9990.72uncharacterized protein At3g49720-like OS=Cucumis melo OX=3656 GN=LOC103494900 P... [more]
A0A5D3E0631.4e-7389.33Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A6J1D5948.3e-7167.88uncharacterized protein At3g49720-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A0A0L1567.0e-7095.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G179070 PE=4 SV=1[more]
A0A6J1K5Y94.3e-6762.69uncharacterized protein At3g49720-like OS=Cucurbita maxima OX=3661 GN=LOC1114919... [more]
Match NameE-valueIdentityDescription
XP_011654354.11.6e-10899.48probable pectin methylesterase CGR3 [Cucumis sativus][more]
KAE8649381.11.6e-10899.48hypothetical protein Csa_011838 [Cucumis sativus][more]
XP_008454502.13.0e-9990.72PREDICTED: uncharacterized protein At3g49720-like [Cucumis melo][more]
XP_038905892.17.4e-9083.51probable pectin methylesterase CGR3 [Benincasa hispida][more]
TYK29433.12.8e-7389.33uncharacterized protein E5676_scaffold655G00250 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT5G65810.13.3e-5653.85unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G49720.11.2e-5351.79unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT3G49720.21.2e-5351.79unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 12..189
e-value: 7.7E-8
score: 33.9
NoneNo IPR availablePANTHERPTHR34208:SF10PECTIN METHYLESTERASE CGR3-RELATEDcoord: 3..195
IPR044689Pectin methyltransferase CGR2/3PANTHERPTHR34208S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE-RELATEDcoord: 3..195
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 80..167

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G11610.1CSPI04G11610.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045488 pectin metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008168 methyltransferase activity