CSPI04G06180 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G06180
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Phytocyanin domain-containing protein
Location	Chr4: 4266228 .. 4267429 (-)
RNA-Seq Expression	CSPI04G06180
Synteny	CSPI04G06180

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAGGAGAAAGAAGAAAGAAGAAAGAAGAAGAAGAAATATATATTCTTTTTTTAAAAAAAGGTTTTAAAAAATACCCATTTTTTTTCTCTCCCCTCTCTCTCTCTTAAGCTCCAAAAGAGAGATTGGCCAAAAAAAAAAAAAAAAAGAATGGACAAAACAATAATGAATTATTGTTATTGGTTAGTAATGATGATGATGATTATGGGTTCATGCGTTGATGCATTCACTCATATTGTTGGTGGAAGCCATGGTTGGAGAGTCCCTGAAAATGATTCCTTTTTTGATCAATGGGCTAAACCAAGAACCTTTGGTGTTGGTGATAGGCTTGGTATATCTCTTTTTCCTTTTACCCTCCCTTTTTGCAATTCAATTTTACTCTCCTAATATTGATCTTATATCTATATACATTTGATAGTATCAATACATACCAACATTACTATAGCATAGCAGTCTCATTGAAGGATTATGTTTTAGAGGTCCAAACTATATTATTTCATCATACGTTTCTAACGTTTTAAGTAATTTTCCTTTTTCAATTGGGGATACGTACATTTACACACGCAGTTTTCCCGTACCGAGCTGGTGCGAACAACCTAGTGACAGTAAAGAAGGCAGACTATGATACATGTGGGGAAGAAGAAGTAATATACATGTATTTTCTAGGGCCAACAGTAGTGAACCTAACAAAGGCTGGTGATTACTATTACTTTGATGGAATTGGAAAGCATTGCGAAGCTGGTCAGAAGCTCCACATCCAAGTTGGTACCAAAGAAGGAAGCTCTGGGTCTGATCCTTTACCATTTAACCTCGAAACTTTTGGAATCCACACCAATTTGGGGCCAGCCCTTTCCCCTCAAGGCCAAATGGATGCCGAATCCGTATCCCAAGCCCAATCCCCATCCGGTACTCCCGCTCACCCATCCAATGCCTTCCTTCTTCTTCCTACACCAATGTTATTGGCTCTTATCATTCCTACCCTTTTTTCCATTTTTCTTTCATTCCCATTTCCATATATTAAGTAATTCCTTTCCCTTTTCTTTTTCTTTTTCTTTTTCTTTTTAATGTGTATCAATCTTTTGTTCTTCCAAATTCCTATTTTCTTGTGTTCCCAACCTTCCAAAGATCAAACTATAAATCCATTTCTTCTGTCTTGGGATGATTCTTGTTTTATTTCAATTCCTTTTATATATATATATATATA

mRNA sequence

GGAGGAGAAAGAAGAAAGAAGAAAGAAGAAGAAGAAATATATATTCTTTTTTTAAAAAAAGGTTTTAAAAAATACCCATTTTTTTTCTCTCCCCTCTCTCTCTCTTAAGCTCCAAAAGAGAGATTGGCCAAAAAAAAAAAAAAAAAGAATGGACAAAACAATAATGAATTATTGTTATTGGTTAGTAATGATGATGATGATTATGGGTTCATGCGTTGATGCATTCACTCATATTGTTGGTGGAAGCCATGGTTGGAGAGTCCCTGAAAATGATTCCTTTTTTGATCAATGGGCTAAACCAAGAACCTTTGGTGTTGGTGATAGGCTTGTTTTCCCGTACCGAGCTGGTGCGAACAACCTAGTGACAGTAAAGAAGGCAGACTATGATACATGTGGGGAAGAAGAAGTAATATACATGTATTTTCTAGGGCCAACAGTAGTGAACCTAACAAAGGCTGGTGATTACTATTACTTTGATGGAATTGGAAAGCATTGCGAAGCTGGTCAGAAGCTCCACATCCAAGTTGGTACCAAAGAAGGAAGCTCTGGGTCTGATCCTTTACCATTTAACCTCGAAACTTTTGGAATCCACACCAATTTGGGGCCAGCCCTTTCCCCTCAAGGCCAAATGGATGCCGAATCCGTATCCCAAGCCCAATCCCCATCCGGTACTCCCGCTCACCCATCCAATGCCTTCCTTCTTCTTCCTACACCAATGTTATTGGCTCTTATCATTCCTACCCTTTTTTCCATTTTTCTTTCATTCCCATTTCCATATATTAAGTAATTCCTTTCCCTTTTCTTTTTCTTTTTCTTTTTCTTTTTAATGTGTATCAATCTTTTGTTCTTCCAAATTCCTATTTTCTTGTGTTCCCAACCTTCCAAAGATCAAACTATAAATCCATTTCTTCTGTCTTGGGATGATTCTTGTTTTATTTCAATTCCTTTTATATATATATATATATA

Coding sequence (CDS)

ATGGACAAAACAATAATGAATTATTGTTATTGGTTAGTAATGATGATGATGATTATGGGTTCATGCGTTGATGCATTCACTCATATTGTTGGTGGAAGCCATGGTTGGAGAGTCCCTGAAAATGATTCCTTTTTTGATCAATGGGCTAAACCAAGAACCTTTGGTGTTGGTGATAGGCTTGTTTTCCCGTACCGAGCTGGTGCGAACAACCTAGTGACAGTAAAGAAGGCAGACTATGATACATGTGGGGAAGAAGAAGTAATATACATGTATTTTCTAGGGCCAACAGTAGTGAACCTAACAAAGGCTGGTGATTACTATTACTTTGATGGAATTGGAAAGCATTGCGAAGCTGGTCAGAAGCTCCACATCCAAGTTGGTACCAAAGAAGGAAGCTCTGGGTCTGATCCTTTACCATTTAACCTCGAAACTTTTGGAATCCACACCAATTTGGGGCCAGCCCTTTCCCCTCAAGGCCAAATGGATGCCGAATCCGTATCCCAAGCCCAATCCCCATCCGGTACTCCCGCTCACCCATCCAATGCCTTCCTTCTTCTTCCTACACCAATGTTATTGGCTCTTATCATTCCTACCCTTTTTTCCATTTTTCTTTCATTCCCATTTCCATATATTAAGTAA

Protein sequence

MDKTIMNYCYWLVMMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRLVFPYRAGANNLVTVKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGTKEGSSGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPAHPSNAFLLLPTPMLLALIIPTLFSIFLSFPFPYIK*

Homology

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BLAST of CSPI04G06180 vs. ExPASy Swiss-Prot
Match: Q41001 (Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 5.1e-15
Identity = 58/181 (32.04%), Postives = 88/181 (48.62%), Query Frame = 0


Query: 9   CYWLVMMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRLVFPYRAGA 68
           C+ L ++ M + S    +T  VG + GW +  +   +  WA  +TF VGD LVF Y AGA
Sbjct: 10  CFLLAIINMALPSLATVYT--VGDTSGWVIGGD---YSTWASDKTFAVGDSLVFNYGAGA 69

Query: 69  NNLVTVKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGT 128
           + +  VK++DY +C     I     G T + L KAG +Y+  G+  H   G KL I+V  
Sbjct: 70  HTVDEVKESDYKSCTSGNSISTDSTGATTIPLKKAGKHYFICGVPGHSTGGMKLSIKV-- 129

Query: 129 KEGSSGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPA--HPSNAFLLL 188
            + SSGS   P          +  P+ S +G   ++    A + + TP   + S+A  L 
Sbjct: 130 -KASSGSSAAP----------SATPSSSGKGSPSSDDTPAATTTTTTPTKQNESSATSLS 172

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BLAST of CSPI04G06180 vs. ExPASy Swiss-Prot
Match: P00302 (Stellacyanin OS=Toxicodendron vernicifluum OX=4013 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 6.7e-15
Identity = 41/101 (40.59%), Postives = 57/101 (56.44%), Query Frame = 0


Query: 28  HIVGGSHGWRVP-ENDSFFD-QWAKPRTFGVGDRLVFPYRAGANNLVTVKKADYDTCGEE 87
           + VG S GW+VP   D  +D +WA  +TF +GD LVF Y    +N+  V + +Y +C + 
Sbjct: 3   YTVGDSAGWKVPFFGDVDYDWKWASNKTFHIGDVLVFKYDRRFHNVDKVTQKNYQSCNDT 62

Query: 88  EVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQV 127
             I  Y  G   +NL   G  YY  G+ KHC+ GQK+HI V
Sbjct: 63  TPIASYNTGBBRINLKTVGQKYYICGVPKHCDLGQKVHINV 103

[Close]

BLAST of CSPI04G06180 vs. ExPASy Swiss-Prot
Match: P42849 (Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 8.8e-15
Identity = 39/105 (37.14%), Postives = 52/105 (49.52%), Query Frame = 0


Query: 30  VGGSHGWRVPENDSFFDQWAKPRTFGVGDRLVFPYRAGANNLVTVKKADYDTCGEEEVIY 89
           VGG   W+ P +  F+  WA  +TF VGD L F + AG +++  V K  +D C +E  I 
Sbjct: 5   VGGDMEWKRPSDPKFYITWATGKTFRVGDELEFDFAAGMHDVAVVTKDAFDNCKKENPIS 64

Query: 90  MYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGTKEGSSG 135
                P  + L   G  YY   +G HC  GQKL I V    G+ G
Sbjct: 65  HMTTPPVKIMLNTTGPQYYICTVGDHCRVGQKLSINVVGAGGAGG 109

[Close]

BLAST of CSPI04G06180 vs. ExPASy Swiss-Prot
Match: O82081 (Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.7e-13
Identity = 53/180 (29.44%), Postives = 84/180 (46.67%), Query Frame = 0


Query: 13  VMMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRLVFPYRAGANNLV 72
           V+   ++G  V A  H +GG  GW V    +    WA  +TF VGD LVF Y A  +++V
Sbjct: 12  VLATTLIGLTV-ATDHTIGGPSGWTV---GASLRTWAAGQTFAVGDNLVFSYPAAFHDVV 71

Query: 73  TVKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGTKEGS 132
            V K ++D+C   + +  +  G ++V LT  G  Y+  G+  HC  G KL + V      
Sbjct: 72  EVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVNVVPTATV 131

Query: 133 SGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQ-----SPSGTPAHPSNAFLLLP 188
           + + PLP  + +         A SP   +  + +         SPS +   PS++  L+P
Sbjct: 132 APTAPLPNTVPSLN-------APSPSSVLPIQPLLPLNPVPVLSPSSSTPLPSSSLPLIP 180

[Close]

BLAST of CSPI04G06180 vs. ExPASy Swiss-Prot
Match: P29602 (Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3)

HSP 1 Score: 75.9 bits (185), Expect = 6.3e-13
Identity = 39/104 (37.50%), Postives = 57/104 (54.81%), Query Frame = 0


Query: 28  HIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRLVFPYRAGANNLVTVK-KADYDTC---- 87
           HIVG + GW VP + +F+ QWA  +TF VGD L F + A A+N+  ++ K  +D C    
Sbjct: 5   HIVGDNTGWSVPSSPNFYSQWAAGKTFRVGDSLQFNFPANAHNVHEMETKQSFDACNFVN 64

Query: 88  GEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQV 127
            + +V       P +  L + G +Y+   +G HC  GQKL I V
Sbjct: 65  SDNDV---ERTSPVIERLDELGMHYFVCTVGTHCSNGQKLSINV 105

[Close]

BLAST of CSPI04G06180 vs. ExPASy TrEMBL
Match: A0A0A0KUU5 (Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G051370 PE=4 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 9.4e-121
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MDKTIMNYCYWLVMMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRL 60
           MDKTIMNYCYWLVMMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRL
Sbjct: 1   MDKTIMNYCYWLVMMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRL 60

Query: 61  VFPYRAGANNLVTVKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQ 120
           VFPYRAGANNLVTVKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQ
Sbjct: 61  VFPYRAGANNLVTVKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQ 120

Query: 121 KLHIQVGTKEGSSGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPAHPS 180
           KLHIQVGTKEGSSGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPAHPS
Sbjct: 121 KLHIQVGTKEGSSGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPAHPS 180

Query: 181 NAFLLLPTPMLLALIIPTLFSIFLSFPFPYIK 213
           NAFLLLPTPMLLALIIPTLFSIFLSFPFPYIK
Sbjct: 181 NAFLLLPTPMLLALIIPTLFSIFLSFPFPYIK 212

[Close]

BLAST of CSPI04G06180 vs. ExPASy TrEMBL
Match: A0A1S4DYV8 (stellacyanin-like OS=Cucumis melo OX=3656 GN=LOC103493116 PE=4 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 2.8e-80
Identity = 157/186 (84.41%), Postives = 161/186 (86.56%), Query Frame = 0


Query: 19  MGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRLVFPYRAGANNLVTVKKAD 78
           MGSCVDAFTHIVGGSHGWRVPEN SF           +   +VFPYRAGANNLV VKKAD
Sbjct: 1   MGSCVDAFTHIVGGSHGWRVPENVSFLINGLNQEPLVL--VIVFPYRAGANNLVAVKKAD 60

Query: 79  YDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGTKEGSSGSDPL 138
           YDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGTKEGSSGSDPL
Sbjct: 61  YDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGTKEGSSGSDPL 120

Query: 139 PFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPAHPSNAFLLLPTPMLLALIIPT 198
           PFNLETFGIHT+LGPALSPQ Q  AES S+ QSP GT   PSNAFLLLPTPMLLALIIPT
Sbjct: 121 PFNLETFGIHTSLGPALSPQPQ--AESESKPQSPPGTATPPSNAFLLLPTPMLLALIIPT 180

Query: 199 LFSIFL 205
           LFSIFL
Sbjct: 181 LFSIFL 182

[Close]

BLAST of CSPI04G06180 vs. ExPASy TrEMBL
Match: A0A6J1ETX6 (stellacyanin-like OS=Cucurbita moschata OX=3662 GN=LOC111437535 PE=4 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 1.6e-64
Identity = 122/184 (66.30%), Postives = 149/184 (80.98%), Query Frame = 0


Query: 12  LVMMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRLVFPYRAGANNL 71
           ++ +MM+MGS V+AFTHIVG SHGWRVP+N +F+D+WAKPRTFGVGD+LVFPYR GANN+
Sbjct: 7   IMTIMMMMGSSVNAFTHIVGASHGWRVPDNVTFYDEWAKPRTFGVGDKLVFPYRPGANNI 66

Query: 72  VTVKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGTKEG 131
           V VKKADY+ CGEE VI MY+LGPT++NLT+AGDYYYFDGIGKHCEAGQKLH+QVG KEG
Sbjct: 67  VAVKKADYEVCGEENVINMYYLGPTILNLTEAGDYYYFDGIGKHCEAGQKLHVQVGLKEG 126

Query: 132 SSGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSP-SGTPAHPSNAFLLLPTPM 191
           +SG+DPLPFNLETFGIHT+L P          ++V+ A +P + T A  +NA  LL    
Sbjct: 127 TSGADPLPFNLETFGIHTSLDP---------TQNVAPAAAPTAATKASHANANALLVPAG 181

Query: 192 LLAL 195
           L A+
Sbjct: 187 LAAV 181

[Close]

BLAST of CSPI04G06180 vs. ExPASy TrEMBL
Match: A0A6J1J846 (stellacyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111483431 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 2.1e-64
Identity = 128/204 (62.75%), Postives = 153/204 (75.00%), Query Frame = 0


Query: 1   MDKTIMNYCYWLVMMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRL 60
           MDK +M     +  MMM+MGS V+AFTHIVG SHGWRVP+N +F+D+WAKPRTFGVGD+L
Sbjct: 1   MDKFMM--IMTITTMMMMMGSSVNAFTHIVGASHGWRVPDNVTFYDEWAKPRTFGVGDKL 60

Query: 61  VFPYRAGANNLVTVKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQ 120
           VFPYR GANN+V VKK DY+ CGEE VI MY+LGPT++NLT+AGDYYYFDGIGKHCEAGQ
Sbjct: 61  VFPYRPGANNIVAVKKTDYEVCGEENVINMYYLGPTILNLTEAGDYYYFDGIGKHCEAGQ 120

Query: 121 KLHIQVGTKEGSSGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPAHPS 180
           KLH+QVG+KEG+SG+DPLPFNLETFGIHTNL P    Q    A + + A   S    + +
Sbjct: 121 KLHVQVGSKEGTSGADPLPFNLETFGIHTNLDPT---QNVGPAAAPTAATKGSNANVNAN 180

Query: 181 NAFLLLPTPMLLALIIPTLFSIFL 205
              LL+P  +     I   FS FL
Sbjct: 181 ANALLVPAGL---AAIAMFFSTFL 196

[Close]

BLAST of CSPI04G06180 vs. ExPASy TrEMBL
Match: A0A6J1J638 (umecyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111481590 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 5.3e-63
Identity = 129/207 (62.32%), Postives = 158/207 (76.33%), Query Frame = 0


Query: 1   MDKTIMNYCYWLVMMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRL 60
           MDK  +     ++  ++ MGS +DAFTHIVGG+HGWRVP+N +FFD+WAKPRTFGVGD+L
Sbjct: 1   MDKMYLALMIGILSCLVFMGS-MDAFTHIVGGNHGWRVPDNVTFFDEWAKPRTFGVGDKL 60

Query: 61  VFPYRAGANNLVTVKKADYDTC--GEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEA 120
           VFPYR GANNL+ VKKADY+TC  GEE+VI MY+LGPT++N+T  GDYYY+DGIGKHCEA
Sbjct: 61  VFPYRPGANNLLAVKKADYETCGQGEEDVINMYYLGPTILNITHPGDYYYYDGIGKHCEA 120

Query: 121 GQKLHIQVGTKEGSSGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPAH 180
           GQKLH+QVG+KEGSSGSDPLPFNL+TFGI T  G  + P     A  V+ A +P  TPA 
Sbjct: 121 GQKLHVQVGSKEGSSGSDPLPFNLQTFGIPTAFGTTVEPPAA--APVVTPAAAPVVTPAA 180

Query: 181 P-SNAFLLLPTPMLLALIIPTLFSIFL 205
             ++ F L  + M   LI+P L SIFL
Sbjct: 181 AMAHKFGLASSAM---LIMPVLLSIFL 201

[Close]

BLAST of CSPI04G06180 vs. NCBI nr
Match: XP_004149757.2 (blue copper protein [Cucumis sativus] >KGN53385.1 hypothetical protein Csa_014752 [Cucumis sativus])

HSP 1 Score: 442.6 bits (1137), Expect = 2.0e-120
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MDKTIMNYCYWLVMMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRL 60
           MDKTIMNYCYWLVMMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRL
Sbjct: 1   MDKTIMNYCYWLVMMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRL 60

Query: 61  VFPYRAGANNLVTVKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQ 120
           VFPYRAGANNLVTVKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQ
Sbjct: 61  VFPYRAGANNLVTVKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQ 120

Query: 121 KLHIQVGTKEGSSGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPAHPS 180
           KLHIQVGTKEGSSGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPAHPS
Sbjct: 121 KLHIQVGTKEGSSGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPAHPS 180

Query: 181 NAFLLLPTPMLLALIIPTLFSIFLSFPFPYIK 213
           NAFLLLPTPMLLALIIPTLFSIFLSFPFPYIK
Sbjct: 181 NAFLLLPTPMLLALIIPTLFSIFLSFPFPYIK 212

[Close]

BLAST of CSPI04G06180 vs. NCBI nr
Match: XP_016901158.1 (PREDICTED: stellacyanin-like [Cucumis melo])

HSP 1 Score: 308.1 bits (788), Expect = 5.7e-80
Identity = 157/186 (84.41%), Postives = 161/186 (86.56%), Query Frame = 0


Query: 19  MGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRLVFPYRAGANNLVTVKKAD 78
           MGSCVDAFTHIVGGSHGWRVPEN SF           +   +VFPYRAGANNLV VKKAD
Sbjct: 1   MGSCVDAFTHIVGGSHGWRVPENVSFLINGLNQEPLVL--VIVFPYRAGANNLVAVKKAD 60

Query: 79  YDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGTKEGSSGSDPL 138
           YDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGTKEGSSGSDPL
Sbjct: 61  YDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGTKEGSSGSDPL 120

Query: 139 PFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPAHPSNAFLLLPTPMLLALIIPT 198
           PFNLETFGIHT+LGPALSPQ Q  AES S+ QSP GT   PSNAFLLLPTPMLLALIIPT
Sbjct: 121 PFNLETFGIHTSLGPALSPQPQ--AESESKPQSPPGTATPPSNAFLLLPTPMLLALIIPT 180

Query: 199 LFSIFL 205
           LFSIFL
Sbjct: 181 LFSIFL 182

[Close]

BLAST of CSPI04G06180 vs. NCBI nr
Match: XP_038878156.1 (stellacyanin-like [Benincasa hispida])

HSP 1 Score: 303.1 bits (775), Expect = 1.8e-78
Identity = 149/204 (73.04%), Postives = 167/204 (81.86%), Query Frame = 0


Query: 1   MDKTIMNYCYWLVMMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRL 60
           MDKT+M  C    ++MM+MGSCV+AFTHIVGGSHGWRVPEN +FFD+W KPRTFGVGDRL
Sbjct: 1   MDKTVMICC----LVMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRL 60

Query: 61  VFPYRAGANNLVTVKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQ 120
           VFPYR GANNL+ VKKADYDTCGEE VI+MYFLGPTV+NLT+ GDYYYFDGIGKHCEAGQ
Sbjct: 61  VFPYRPGANNLLAVKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQ 120

Query: 121 KLHIQVGTKEGSSGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPAHPS 180
           KLHIQVG+KEG+SGSDP+PFNLETFGIHTNLGPA++P  Q  A         +    HPS
Sbjct: 121 KLHIQVGSKEGTSGSDPMPFNLETFGIHTNLGPAMAPTAQTQA---------AANATHPS 180

Query: 181 NAFLLLPTPMLLALIIPTLFSIFL 205
           NAFLL   P +L  II  LFSIFL
Sbjct: 181 NAFLL--PPPILPPIIAFLFSIFL 189

[Close]

BLAST of CSPI04G06180 vs. NCBI nr
Match: KAG6577158.1 (hypothetical protein SDJN03_24732, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 257.3 bits (656), Expect = 1.2e-64
Identity = 124/188 (65.96%), Postives = 149/188 (79.26%), Query Frame = 0


Query: 12  LVMMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRLVFPYRAGANNL 71
           + MMMM+MGS V+AFTHIVG SHGWRVP+N +F+D+WAKPRTFGVGD+LVFPYR GANN+
Sbjct: 10  ITMMMMMMGSSVNAFTHIVGASHGWRVPDNVTFYDEWAKPRTFGVGDKLVFPYRPGANNI 69

Query: 72  VTVKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGTKEG 131
           V VKKADY+ CGEE VI MY+LGPT++NLT+AGDYYYFDGIGKHCEAGQKLH+QVG KEG
Sbjct: 70  VAVKKADYEVCGEENVINMYYLGPTILNLTEAGDYYYFDGIGKHCEAGQKLHVQVGLKEG 129

Query: 132 SSGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPAHPSNAFLLLPTPML 191
           +SG+DPLPFNLETFGIHT+L P    Q    A + + A   S   A+ +NA L+      
Sbjct: 130 TSGADPLPFNLETFGIHTSLDPT---QNVAPAAAPTAAMKASHANAN-ANALLVPAGLAA 189

Query: 192 LALIIPTL 200
           +A+   TL
Sbjct: 190 VAMFFSTL 193

[Close]

BLAST of CSPI04G06180 vs. NCBI nr
Match: XP_023552874.1 (stellacyanin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 256.9 bits (655), Expect = 1.5e-64
Identity = 124/187 (66.31%), Postives = 154/187 (82.35%), Query Frame = 0


Query: 16  MMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRLVFPYRAGANNLVTVK 75
           MM+MGS V+AFTHIVG SHGWRVP+N +F+D+WAKPRTFGVGD+LVFPYR GANN+V VK
Sbjct: 1   MMMMGSSVNAFTHIVGASHGWRVPDNVTFYDEWAKPRTFGVGDKLVFPYRPGANNIVAVK 60

Query: 76  KADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGTKEGSSGS 135
           KADY+ CGEE VI MY+LGPT++NLT+AGDYYYFDGIGKHCEAGQKLH+QVG+K+G+SG+
Sbjct: 61  KADYEVCGEENVINMYYLGPTILNLTEAGDYYYFDGIGKHCEAGQKLHVQVGSKDGTSGA 120

Query: 136 DPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSP-SGTPAHPSNA-FLLLPTPM-LL 195
           DPLPFNLETFGIHT+L P         +++V+ A +P + T A  +NA  LL+PT +  +
Sbjct: 121 DPLPFNLETFGIHTSLDP---------SQNVAPAAAPTAATKASHANANALLVPTGLAAI 178

Query: 196 ALIIPTL 200
           A+   TL
Sbjct: 181 AMFFSTL 178

[Close]

BLAST of CSPI04G06180 vs. TAIR 10
Match: AT4G31840.1 (early nodulin-like protein 15 )

HSP 1 Score: 85.9 bits (211), Expect = 4.3e-17
Identity = 51/140 (36.43%), Postives = 73/140 (52.14%), Query Frame = 0


Query: 21  SCVDAFTHIVGGSHG-WRVPENDSF-FDQWAKPRTFGVGDRLVFPYRAGANNLVTVKKAD 80
           S V+A    VGG  G W++P + SF F++WA+   F VGD +VF Y AG ++++ V +  
Sbjct: 20  SSVNANEVTVGGKSGDWKIPPSSSFSFNEWAQKARFKVGDFIVFKYEAGKDSVLQVTREA 79

Query: 81  YDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGTKEGSSGS-DP 140
           Y+ C        Y  G T V L +AG  Y+  G   HC+ GQKL + V T   S+ S  P
Sbjct: 80  YEKCNTTSPKASYTDGNTKVKLDQAGPVYFVSGTEGHCQKGQKLRLVVITPRNSAFSPGP 139

Query: 141 LPFNLETFGIHTNLGPALSP 158
            P   +        GPA++P
Sbjct: 140 SPSEFD--------GPAVAP 151

[Close]

BLAST of CSPI04G06180 vs. TAIR 10
Match: AT5G53870.1 (early nodulin-like protein 1 )

HSP 1 Score: 79.0 bits (193), Expect = 5.3e-15
Identity = 51/162 (31.48%), Postives = 76/162 (46.91%), Query Frame = 0


Query: 21  SCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRLVFPYRAGANNLVTVKKADYD 80
           S  DA+   VGG+  W V      ++ WA+   F V D L F Y  G++++  V KAD+D
Sbjct: 23  SVADAWRFNVGGNGAW-VTNPQENYNTWAERNRFQVNDSLYFKYAKGSDSVQQVMKADFD 82

Query: 81  TCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHI---QVGTKEGSSGSDP 140
            C     I  +  G +VV L ++G +Y+  G   HC+ GQKL +    V  +  +    P
Sbjct: 83  GCNVRNPIKNFENGESVVTLDRSGAFYFISGNQDHCQKGQKLIVVVLAVRNQPSAPAHSP 142

Query: 141 LPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPAHP 180
           +P    T    ++    +SP     A S SQ    S +PA P
Sbjct: 143 VPSVSPTQPPKSH--SPVSPVAPASAPSKSQPPRSSVSPAQP 181

[Close]

BLAST of CSPI04G06180 vs. TAIR 10
Match: AT2G32300.1 (uclacyanin 1 )

HSP 1 Score: 76.6 bits (187), Expect = 2.6e-14
Identity = 53/180 (29.44%), Postives = 84/180 (46.67%), Query Frame = 0


Query: 13  VMMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRLVFPYRAGANNLV 72
           V+   ++G  V A  H +GG  GW V    +    WA  +TF VGD LVF Y A  +++V
Sbjct: 12  VLATTLIGLTV-ATDHTIGGPSGWTV---GASLRTWAAGQTFAVGDNLVFSYPAAFHDVV 71

Query: 73  TVKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGTKEGS 132
            V K ++D+C   + +  +  G ++V LT  G  Y+  G+  HC  G KL + V      
Sbjct: 72  EVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVNVVPTATV 131

Query: 133 SGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQ-----SPSGTPAHPSNAFLLLP 188
           + + PLP  + +         A SP   +  + +         SPS +   PS++  L+P
Sbjct: 132 APTAPLPNTVPSLN-------APSPSSVLPIQPLLPLNPVPVLSPSSSTPLPSSSLPLIP 180

[Close]

BLAST of CSPI04G06180 vs. TAIR 10
Match: AT3G18590.1 (early nodulin-like protein 5 )

HSP 1 Score: 76.3 bits (186), Expect = 3.4e-14
Identity = 54/176 (30.68%), Postives = 82/176 (46.59%), Query Frame = 0


Query: 12  LVMMMMIMGSCVDAFTHIVGGSHGWRVPENDSF---FDQWAKPRTFGVGDRLVFPYRAGA 71
           + ++   M SCV +    VGG +GW VP++ +    F+QWA    F VGD L F Y    
Sbjct: 11  MFLVTFYMFSCVSSTEFEVGGENGWIVPKSKTLGDAFNQWASDNRFKVGDTLRFKYT--K 70

Query: 72  NNLVTVKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGT 131
           ++++ V + +Y  C   +         TV  L + G +Y+  G+  HCE GQK+ ++V  
Sbjct: 71  DSVLVVSEEEYKKCKATKPQLYSNNEDTVFKLDRPGLFYFISGVSGHCEKGQKMIVKVME 130

Query: 132 KEGSSGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTP-AHPSNAF 184
            E S+ S P               P+ S      + S S +  P+ TP A  SNAF
Sbjct: 131 TESSTESPP---------------PSSS------SSSSSSSSLPASTPKAKKSNAF 163

[Close]

BLAST of CSPI04G06180 vs. TAIR 10
Match: AT5G20230.1 (blue-copper-binding protein )

HSP 1 Score: 74.3 bits (181), Expect = 1.3e-13
Identity = 50/190 (26.32%), Postives = 79/190 (41.58%), Query Frame = 0


Query: 14  MMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRLVFPYRAGANNLVT 73
           ++++     V A  + VG    W  P +  F+  WA  +TF VGD L F + AG +++  
Sbjct: 11  LVLVFAAVVVFAEDYDVGDDTEWTRPMDPEFYTTWATGKTFRVGDELEFDFAAGRHDVAV 70

Query: 74  VKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGTKEGSS 133
           V +A ++ C +E+ I    + P  + L   G  Y+   +G HC  GQKL I V     + 
Sbjct: 71  VSEAAFENCEKEKPISHMTVPPVKIMLNTTGPQYFICTVGDHCRFGQKLSITVVAAGATG 130

Query: 134 GSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPAHPSNAFLLLPTPMLLA 193
           G+ P        G   + G    P     A   +     SGT     NA   L     L 
Sbjct: 131 GATPGAGATPAPGSTPSTGGTTPP----TAGGTTTPSGSSGTTTPAGNAASSLGGATFLV 190

Query: 194 LIIPTLFSIF 204
             +  + ++F
Sbjct: 191 AFVSAVVALF 196

The following BLAST results are available for this feature:

BLAST of CSPI04G06180 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q41001	5.1e-15	32.04	Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
P00302	6.7e-15	40.59	Stellacyanin OS=Toxicodendron vernicifluum OX=4013 PE=1 SV=1	[more]
P42849	8.8e-15	37.14	Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1	[more]
O82081	3.7e-13	29.44	Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1	[more]
P29602	6.3e-13	37.50	Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3	[more]

BLAST of CSPI04G06180 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KUU5	9.4e-121	100.00	Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G051370...	[more]
A0A1S4DYV8	2.8e-80	84.41	stellacyanin-like OS=Cucumis melo OX=3656 GN=LOC103493116 PE=4 SV=1	[more]
A0A6J1ETX6	1.6e-64	66.30	stellacyanin-like OS=Cucurbita moschata OX=3662 GN=LOC111437535 PE=4 SV=1	[more]
A0A6J1J846	2.1e-64	62.75	stellacyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111483431 PE=4 SV=1	[more]
A0A6J1J638	5.3e-63	62.32	umecyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111481590 PE=4 SV=1	[more]

BLAST of CSPI04G06180 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149757.2	2.0e-120	100.00	blue copper protein [Cucumis sativus] >KGN53385.1 hypothetical protein Csa_01475...	[more]
XP_016901158.1	5.7e-80	84.41	PREDICTED: stellacyanin-like [Cucumis melo]	[more]
XP_038878156.1	1.8e-78	73.04	stellacyanin-like [Benincasa hispida]	[more]
KAG6577158.1	1.2e-64	65.96	hypothetical protein SDJN03_24732, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023552874.1	1.5e-64	66.31	stellacyanin-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI04G06180 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31840.1	4.3e-17	36.43	early nodulin-like protein 15	[more]
AT5G53870.1	5.3e-15	31.48	early nodulin-like protein 1	[more]
AT2G32300.1	2.6e-14	29.44	uclacyanin 1	[more]
AT3G18590.1	3.4e-14	30.68	early nodulin-like protein 5	[more]
AT5G20230.1	1.3e-13	26.32	blue-copper-binding protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 36..120 e-value: 1.6E-18 score: 66.5
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 26..128 score: 31.324223
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 26..137 e-value: 4.3E-28 score: 99.7
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 25..126
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 3..143
None	No IPR available	PANTHER	PTHR33021:SF264	OS05G0570900 PROTEIN	coord: 3..143
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..22 score: 5.0

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G06180.1	CSPI04G06180.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009055	electron transfer activity