CSPI04G04940 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G04940
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionSec14p-like phosphatidylinositol transfer family protein
LocationChr4: 3289377 .. 3294592 (-)
RNA-Seq ExpressionCSPI04G04940
SyntenyCSPI04G04940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCGAGATTTCTCCCTCTTTTTCGCTGCCCAAATAATCGTGGCCTTCTCAGATTTGTGGTTGCACCCACCGTTCTTCTTCACCTGCTCCATTTTCGAGACGAACAAACAAAAGCATTCAACCAGTGGATTCTCTTAATTATCACTATTTTAAATCCGTTTTTCTTTTCCCTGAGGAAGTTTTCTCTTTCGGTTTCTTCATCCCTCTACTTGCTTCTGGTGAGTTTTCTGTAAAATTTTGTTCTATTTTTCTGTTTTTTGTTGGAATCCTGAGGTTTCTTGCTTCCACTATACTTTCGCGTGTGTTTTTGGATGATTGAGCTTTACTGTGATCTTGTCTGAGTGTTTTTGGAGTAGATCGGATTGTTGCGAGTTCCGAGTTTTGCGTTGGTGATTGTCGATTTTTATCGGTTTGACTCTTGGTTACTTTATCTCGTTTTGGAATTGGATTCGCTAATTACTGTCTTTCTCTTAATTTTTCCTGTTTTATCGGTTCGTTTGTTCTTTAAACTCAACGGCGTTGGTTTGTTGTTTGGATTTTTGACATTTGTGTTAAACTCTGATTCTGTGATTCTAGTTTGGTATAGGATTGAGATTGATCTTGTTGATTTTGTTCGTTAGATAGTTACATTTTTCAATAGTATTGGCTGGTTGAACTTAGATAGGGAATGCCAATCGATTTATCAACATACCGGAAATTAATTTTGCAGTCTTAGAGTTTATAGTTTTTTAGGATACGATCTATTCCATCCATGGACTGGATTGTTTATTGCCTGTTCTGAATCTTGACCTAAGGAGGATAAAACTTATAGCAGCGTCCAGAAATTGAGCTGATAAACAAATAATTGTTCTCTTCACACTTAAAATCGAATCATGAGATCATTGAGAATTTAGGTACTGATTAGGAAGTAGTTTAGTTTTCCTCGTTTACTAAATAGAATTTTAACAAAAGCACTCGTGGAAAAATGATTCAATCAGACAGACATGTCCTATACCCAAGGGAACAAATTGGTTGTGTATTAAATCATTCTTGGCCTAAAATTAATTATATATCTTCTTAACATTGTGGTGGGCCGTGGCTTCAAATAATTTATATCTTTTTAAAGAAAACTTTACTAGTTTAATTTATTGTGGACAGCTTTTTTCTCGTCTTAATTGTAAAAATATAAATGTATATTTTTAAAAAAACTTTAATCGAACTTATTTGTAGAGAAAATAGAGAAATATTCAGTCACGTTCTTTTAATTTGAGAATTTCAAATGCTTCTCATTTTTTGAAAATGATTTACACGCTCCTTAATAACCAATCGACAAAACTTCTAGGTAGCCTTGCATTCCGTCATCTAATAGTGTAAAATAGTTAGCCAATGTCGCCAATTTGAATAATTTTGCTATTGTTTGTTCCTATTTAATCTCAATGTAGCAAACTATTTTAATGGTTAAAGATTTCGTTCAACATTAGCATCTTGGTTTTATTTATTCTATTATGATCAGTATAAGGTAATGCTGTTCTCCATAATAGCTAGTCTAGCTACTGATTTTTTTTATATATATATCTTGTCCTCACTCAAATAGCCAGTGAAGTGTTTAAATAATGACTTTGTTTGTAGGTTATCGTGCTGCACATTGTGCTCCATTGCTAATTGATATGTTTCGTTCAAAAAAACATTCTCAAAACAACGATTCTACACAAGAAGAAGGGAAGGTACTAGTTAATTCAATTTTTATAGACAACAGCTTTCATTTTGTTAGTTTGAGTGACTTGATATATATGTGTGTGTATGTATTCTTCTTAGTCTCTATCATATCTGTGGAACTCTATCTATTGAAGTCAAACTATAGTTTGGGTGATATTTTCGGGGAAAATTAAAATCCTGAAGTTTTGGGGTCAGTTAAAATAACTCCGTTCCATAGAAGTAATATATCCCATCTCAAATTTAATTTTCGTACCATCTTGAAGGAAATTTTAGAACCAATTCTCATTTATGAAGTCCAGGACCCAATTCATATAAACTAGGGTCATGCCTTCACACTTGAGCCTGTCTACAAGAAAATATCTAAGACTTTAGGCATGTAGCTTTTAACATTTAAAGACCAAAAGCATCTTGGTTTTTAGCTTGATGCGTAAATAACAGAACAAATAATGCATCACGTACCCCATCCAAATTAGTAATTTTCTTGAATTATTGATGCCATATGTACGTTTCTTAATTTCTGTCCTGCATCACCGTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTGTCCTGCATCCCCGTTTTTTTTTTTTTTTTTTTTTTGATGAGGCGTTAATACAACAAGTTTTCAGATATAAAGATCTTTTTCATTAGAAGATTGCCAACTTTTTATCTTAGACTATTTAGTTGAAGATAAAAAAAAAAAAAAAAAGTTAATGACTCGAATTTTTGAACGCCAGGAACAGATTAGGGCCGGATCAGTTAAGGAAGGTGTGACTAATTATATTTATGTTTCTCATACAGATCAATGAACTTAGAGCTGCCCTAGGGCCTCTGTCTAGTCGAAGCTCAAAGTATTGCACCGATGCATGTCTGAGGAGATACTTGATAGCTCGAAACTGGAACGTTGAGAAGTCTAGAAAAATGCTTGAAGAGACGCTCAAATGGAGGGCAGCTTACAAGCCCGAAGAAATCGGTTGGGTAAGTTAAATTATTAATTATATAATTTTATATCTTCAAACATCTATCACAAACAGTTAATATATATTATTCACCTTGGTTCGCATACTTTCAAAATATCATTTTTGTCTTAGAATATCATTGTTGTCCCTAAAATTGGTCAGTTTTTAGAAGTTCGGAGAAAAAATTGTCATTTTGATAGTTCAGAGAATAAAATGGACATTTAAACAGTACACGTACCAAAAAAATTTATATTGAAAGTTGATATGACTGTTAAAATATTTCTTAATAAAATTCTCTCTTATATGCATGGTTGCATTCTATGGAATCAAAAGATTGTTTAAGAAGCAATTTTAATTTATCGTACAGGATGAAGTAGCATTTGAAGGCGAAACTGGGAAAGTGTCGAGAGCAAATTTTTACGATCGACATGGAAGATCTGTGCTCATAATGAGACCAGGAATGCAGGTATGAGCTGGACTCCCCATCAATATTAATGGAACTCGTACTTCCATTATTACTACTATTGAAATTCCATTTTTCTTTATAATAACAAATCTCAAATCACCGGATCCAAATTTTAAGATCTTAAACTAAAAGGCTATGATTTGTTTAATCTTTTCCGTTATAGCAATAAAAAGGACAGTATGAAAATTGGAATAGAGAGAAGCATCTGAATGATCATCTAGGTTTAGATAAGATATTTATCAAATTCTACATGACTAAACTAAGGCATTATATCAAATCCTCCCACCACTCATCAGAAAGAAATAATAAGTAGACAAATTTTTAGAAAAATAAAAAGTACAAAGTATAGAAGTGGTCCATTCATTTGCAAATTTAAGTTTTGATCTTTTTTGTGTTTTGCATTCACTTTCTGATTTTAGTGACAGTTCCTATTTTTGAAATCTCTCAGAACACAACTCCATCAGAAGCCAGTGTGCGGCATTTGGTCTATCTTTTAGAGAATGCGATTATGAACCTTGGTGAGGGTCAGGAACAAATGTGTTGGTTAATAGATTTCACCGGATTTACAATGAAAACCAATGTCTCTGTCAAAATAGCCGCAGATATTATCAATGTCCTTCAGAACCATTACCCCGAGAGACTTGCCTTTGCTTTCCTCTACAATCCCCCCAAGTTTTTTCAAGCCTTCTGGAAGGTATGTGCTCTTTAATGCCATCATTCATATACATGCATCCATACAATTGAATATTCCTACTGCTTTTAGCTTTCTCTCAATAGTGCGTAATATTTCAAGACAATCTATGGATCTGAATACCAACAAAAAGTAATCAGTATGTACACTAAGGATAAAGTTTAAGTGTGCCTTTATTAATTCAAATAGTAAGTTAATTCCCATGATTTTCTTTTCAAGTATCATCAATATTAATATCTTATTTATAGAAAATGTGGACACGATCGAACAATCAACTCCAAACTTCCTTCCTATTTTGTCACGAATGAAATAACCAGTAGATTGATTGTTCCAATTTTTATAGGCTATAAAGTACTTCTTGGATCCAAAGACATTTCAAAAGGTAAAGTTTGTAAATCCGAAAGACAAAGGAAGTGTGGAGCTGATGAAATCACACTTCGACATGGAAAATCTTCCCAGTGTGTTTGGAGGTAAAGCCACACTCGAATATGATCATCAGCAGTTCTCCCAGATGATGGGCCAAGACGAGCTTAAAGCTGCCCAATTCTGGGGCTTTGATGAGAAGGCCCAACACAGTGTTAATGGGCCTTCCTCTGGCCCAGAAGTTGGACCTGAGCCCGTTACGTCAACTAACTGACTAACTAACTAACTGGTATGTCTCTCATGCCTCTCCCCATTTTACAGCCCATTTTGAAAGTTAAATAATAATCAACTTCTCTTTAATGCAAAGGAGATGATGTTTGCTCATATCCATAAGTAATTAGTCTCTGCCAACCAACCATGAGAGACTATTGTTATATATATTGTGTTAACTTACACAGTAGAATTATCAAGAGAGCCCAATAATGTTTTCTAAAAAAAAAAGAATAAATACATGGAGGGATACCTTTTGTTGATTTCTCTCTTGTAGCTGTCTCTCTTTAGAATAGAAGAAAATTACTATAATTTGTTTACTTTGTTGCATATTTTTTTCCATGTATCATTTATAATAAACTTAGAGAGACCCATGCCTTTAAGTTTAGGCTTCGAATTACAGCCAATTTCATATATCATATATTCCTTTTCTGGCCTAACTTGATCTATTAAAATGTCAGAGTATGTTATTATCATAATTATTACTCAACTCAACTCCATGCATCAAAAGCTCATATGTCAAACGTCGGCAGCTACAGGATGTAACTAACTGTGATGCCATAGACCAATATAGCTCTTGAAACCATGTCTATAGGCCAACCAAAACGGCCTATATATGAGTTTTTGAGATATTTAAATAATAATAATGATAATAATAAAGATAGAAAAGGAAA

mRNA sequence

GCGAGATTTCTCCCTCTTTTTCGCTGCCCAAATAATCGTGGCCTTCTCAGATTTGTGGTTGCACCCACCGTTCTTCTTCACCTGCTCCATTTTCGAGACGAACAAACAAAAGCATTCAACCAGTGGATTCTCTTAATTATCACTATTTTAAATCCGTTTTTCTTTTCCCTGAGGAAGTTTTCTCTTTCGGTTTCTTCATCCCTCTACTTGCTTCTGGTTATCGTGCTGCACATTGTGCTCCATTGCTAATTGATATGTTTCGTTCAAAAAAACATTCTCAAAACAACGATTCTACACAAGAAGAAGGGAAGATCAATGAACTTAGAGCTGCCCTAGGGCCTCTGTCTAGTCGAAGCTCAAAGTATTGCACCGATGCATGTCTGAGGAGATACTTGATAGCTCGAAACTGGAACGTTGAGAAGTCTAGAAAAATGCTTGAAGAGACGCTCAAATGGAGGGCAGCTTACAAGCCCGAAGAAATCGGTTGGGATGAAGTAGCATTTGAAGGCGAAACTGGGAAAGTGTCGAGAGCAAATTTTTACGATCGACATGGAAGATCTGTGCTCATAATGAGACCAGGAATGCAGAACACAACTCCATCAGAAGCCAGTGTGCGGCATTTGGTCTATCTTTTAGAGAATGCGATTATGAACCTTGGTGAGGGTCAGGAACAAATGTGTTGGTTAATAGATTTCACCGGATTTACAATGAAAACCAATGTCTCTGTCAAAATAGCCGCAGATATTATCAATGTCCTTCAGAACCATTACCCCGAGAGACTTGCCTTTGCTTTCCTCTACAATCCCCCCAAGTTTTTTCAAGCCTTCTGGAAGGCTATAAAGTACTTCTTGGATCCAAAGACATTTCAAAAGGTAAAGTTTGTAAATCCGAAAGACAAAGGAAGTGTGGAGCTGATGAAATCACACTTCGACATGGAAAATCTTCCCAGTGTGTTTGGAGGTAAAGCCACACTCGAATATGATCATCAGCAGTTCTCCCAGATGATGGGCCAAGACGAGCTTAAAGCTGCCCAATTCTGGGGCTTTGATGAGAAGGCCCAACACAGTGTTAATGGGCCTTCCTCTGGCCCAGAAGTTGGACCTGAGCCCGTTACGTCAACTAACTGACTAACTAACTAACTGGTATGTCTCTCATGCCTCTCCCCATTTTACAGCCCATTTTGAAAGTTAAATAATAATCAACTTCTCTTTAATGCAAAGGAGATGATGTTTGCTCATATCCATAAGTAATTAGTCTCTGCCAACCAACCATGAGAGACTATTGTTATATATATTGTGTTAACTTACACAGTAGAATTATCAAGAGAGCCCAATAATGTTTTCTAAAAAAAAAAGAATAAATACATGGAGGGATACCTTTTGTTGATTTCTCTCTTGTAGCTGTCTCTCTTTAGAATAGAAGAAAATTACTATAATTTGTTTACTTTGTTGCATATTTTTTTCCATGTATCATTTATAATAAACTTAGAGAGACCCATGCCTTTAAGTTTAGGCTTCGAATTACAGCCAATTTCATATATCATATATTCCTTTTCTGGCCTAACTTGATCTATTAAAATGTCAGAGTATGTTATTATCATAATTATTACTCAACTCAACTCCATGCATCAAAAGCTCATATGTCAAACGTCGGCAGCTACAGGATGTAACTAACTGTGATGCCATAGACCAATATAGCTCTTGAAACCATGTCTATAGGCCAACCAAAACGGCCTATATATGAGTTTTTGAGATATTTAAATAATAATAATGATAATAATAAAGATAGAAAAGGAAA

Coding sequence (CDS)

ATGTTTCGTTCAAAAAAACATTCTCAAAACAACGATTCTACACAAGAAGAAGGGAAGATCAATGAACTTAGAGCTGCCCTAGGGCCTCTGTCTAGTCGAAGCTCAAAGTATTGCACCGATGCATGTCTGAGGAGATACTTGATAGCTCGAAACTGGAACGTTGAGAAGTCTAGAAAAATGCTTGAAGAGACGCTCAAATGGAGGGCAGCTTACAAGCCCGAAGAAATCGGTTGGGATGAAGTAGCATTTGAAGGCGAAACTGGGAAAGTGTCGAGAGCAAATTTTTACGATCGACATGGAAGATCTGTGCTCATAATGAGACCAGGAATGCAGAACACAACTCCATCAGAAGCCAGTGTGCGGCATTTGGTCTATCTTTTAGAGAATGCGATTATGAACCTTGGTGAGGGTCAGGAACAAATGTGTTGGTTAATAGATTTCACCGGATTTACAATGAAAACCAATGTCTCTGTCAAAATAGCCGCAGATATTATCAATGTCCTTCAGAACCATTACCCCGAGAGACTTGCCTTTGCTTTCCTCTACAATCCCCCCAAGTTTTTTCAAGCCTTCTGGAAGGCTATAAAGTACTTCTTGGATCCAAAGACATTTCAAAAGGTAAAGTTTGTAAATCCGAAAGACAAAGGAAGTGTGGAGCTGATGAAATCACACTTCGACATGGAAAATCTTCCCAGTGTGTTTGGAGGTAAAGCCACACTCGAATATGATCATCAGCAGTTCTCCCAGATGATGGGCCAAGACGAGCTTAAAGCTGCCCAATTCTGGGGCTTTGATGAGAAGGCCCAACACAGTGTTAATGGGCCTTCCTCTGGCCCAGAAGTTGGACCTGAGCCCGTTACGTCAACTAACTGA

Protein sequence

MFRSKKHSQNNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVEKSRKMLEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTPSEASVRHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPERLAFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFGGKATLEYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEPVTSTN*
Homology
BLAST of CSPI04G04940 vs. ExPASy Swiss-Prot
Match: Q9UU99 (CRAL-TRIO domain-containing protein C23B6.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC23B6.04c PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.3e-29
Identity = 88/253 (34.78%), Postives = 127/253 (50.20%), Query Frame = 0

Query: 9   QNNDSTQEEGKINELRAALG----PLSSRSSK----------YCTDACLRRYLIARNWNV 68
           +N  S QEE     L+  L     P++S SSK          + T  C+ RYL A  W+V
Sbjct: 590 RNLTSEQEEMYEEVLKYCLELKEIPVASNSSKKTDLIELERLWLTRECILRYLRATKWHV 649

Query: 69  EKSRKMLEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTT 128
             ++K + +TL WR  +    +  DE+  E  TGK      YD+ GR  L + P  QNT 
Sbjct: 650 SNAKKRIVDTLVWRRHFGVNNMDPDEIQEENATGKQVLLG-YDKDGRPCLYLYPARQNTK 709

Query: 129 PSEASVRHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPE 188
            S   +RHLV+ LE AI  +  G E +  LI+F   + ++N SV    +++N+LQ HY E
Sbjct: 710 TSPLQIRHLVFSLECAIDLMPPGVETLALLINFKSSSNRSNPSVGQGKEVLNILQTHYCE 769

Query: 189 RLAFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVF 248
           RL  A + N P     F+K I  F+DP T +K+KF  P D+        +   + L S F
Sbjct: 770 RLGRALVINIPWAVWGFFKLISPFIDPITREKLKFNEPLDR--------YVPKDQLDSNF 829

BLAST of CSPI04G04940 vs. ExPASy Swiss-Prot
Match: Q55CU8 (Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=pitC PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 8.0e-27
Identity = 74/219 (33.79%), Postives = 114/219 (52.05%), Query Frame = 0

Query: 37  YCTDACLRRYLIARNWNVEKSRKMLEETLKWRAAYKPEEIG-WDEVAFEGETGKVSRANF 96
           +CTD C  RYL ARN+ V KS KML +TL+WR  ++P++I    ++   G  G V   N 
Sbjct: 70  FCTDMCFLRYLRARNYIVSKSEKMLRDTLEWRKKFRPQDIQLGGDIREIGSAGCV-YVNK 129

Query: 97  YDRHGRSVL--IMRPGMQNTTPSEASVRHLVYLLENAIMNLGE--GQEQMCWLIDFTGFT 156
            D+ GR ++  + R       PSE   ++LVY LE     + E  G EQ C+++D+  F 
Sbjct: 130 RDKKGRPIIFAVPRNDTLKNVPSELKFKNLVYWLEQGFSRMDEPKGIEQFCFIVDYKDFG 189

Query: 157 MKTNVSVKIAADIINVLQNHYPERLAFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVN 216
              N+ +K   + ++ L +H PER+  +   +PP  F   WK I  FL+  T  KV+F+N
Sbjct: 190 -SGNMDMKTNLEAMHFLLDHCPERMGQSLFLDPPALFWFAWKIISPFLNEVTLSKVRFIN 249

Query: 217 PK---DKGSVELMKSHFDMENLPSVFGGKATLEYDHQQF 248
            K    K +   +  + D+ENL    GG     Y+  ++
Sbjct: 250 SKKVDGKRTFAELLEYVDIENLEQNLGGNLDYNYNIDEY 286

BLAST of CSPI04G04940 vs. ExPASy Swiss-Prot
Match: P53860 (Phosphatidylinositol transfer protein PDR16 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PDR16 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.2e-24
Identity = 79/257 (30.74%), Postives = 115/257 (44.75%), Query Frame = 0

Query: 6   KHSQNNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVEKSRKMLEETL 65
           KH  N D      + N+    L PL      + T  C  RYL A  W ++     +  TL
Sbjct: 53  KHFSNPDLKVYTSEKNKSEDDLKPLEEEEKAWLTRECFLRYLRATKWVLKDCIDRITMTL 112

Query: 66  KWRAAYKPEEIG--------WDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTPSE 125
            WR  +    +G         D VA E E+GK      Y+   R +L ++PG QNT  S 
Sbjct: 113 AWRREFGISHLGEEHGDKITADLVAVENESGKQVILG-YENDARPILYLKPGRQNTKTSH 172

Query: 126 ASVRHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNV-------SVKIAADIINVLQN 185
             V+HLV++LE  I  +  GQ+ +  LIDF  +     V        + +  +++++LQ 
Sbjct: 173 RQVQHLVFMLERVIDFMPAGQDSLALLIDFKDYPDVPKVPGNSKIPPIGVGKEVLHILQT 232

Query: 186 HYPERLAFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENL 245
           HYPERL  A L N P     F K I  F+DP T +K+ F  P  K        +     L
Sbjct: 233 HYPERLGKALLTNIPWLAWTFLKLIHPFIDPLTREKLVFDEPFVK--------YVPKNEL 292

Query: 246 PSVFGGKATLEYDHQQF 248
            S++GG    +Y+H  +
Sbjct: 293 DSLYGGDLKFKYNHDVY 300

BLAST of CSPI04G04940 vs. ExPASy Swiss-Prot
Match: P53844 (Phosphatidylinositol transfer protein PDR17 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PDR17 PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 5.4e-23
Identity = 86/274 (31.39%), Postives = 128/274 (46.72%), Query Frame = 0

Query: 6   KHSQNNDSTQEEGKINEL----RAALGPLSSRSSKYCTDACLRRYLIARNWNVEKSRKML 65
           +H Q++D    E  +N+L     A   PLS     + +  C  RYL A  WN   + K L
Sbjct: 55  RHFQDDDLKLPE-NLNDLDNGTHANDRPLSDWEKFWLSRECFLRYLRANKWNTANAIKGL 114

Query: 66  EETLKWR-------AAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTT 125
            +TL WR            + +  D+VA E ETGK     F D   R +  M+ G QNT 
Sbjct: 115 TKTLVWRREIGLTHGKEDKDPLTADKVAVENETGKQVILGF-DNAKRPLYYMKNGRQNTE 174

Query: 126 PSEASVRHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVS-----VKIAADIINVLQ 185
            S   V+ LVY++E A     +G E++  L+DF  +     ++     + IA   +NV+Q
Sbjct: 175 SSFRQVQELVYMMETATTVAPQGVEKITVLVDFKSYKEPGIITDKAPPISIARMCLNVMQ 234

Query: 186 NHYPERLAFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMEN 245
           +HYPERLA   L N P F  AF K +  FLDP T  K  F  P         ++H +   
Sbjct: 235 DHYPERLAKCVLINIPWFAWAFLKMMYPFLDPATKAKAIFDEP--------FENHIEPSQ 294

Query: 246 LPSVFGGKATLEYDHQQF--SQMMGQDELKAAQF 262
           L +++ G    +Y H+ +    +   D+L+  +F
Sbjct: 295 LDALYNGLLDFKYKHEVYWPDMVKKVDDLRLKRF 318

BLAST of CSPI04G04940 vs. ExPASy Swiss-Prot
Match: P45816 (SEC14 cytosolic factor OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=SEC14 PE=3 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 4.6e-14
Identity = 70/247 (28.34%), Postives = 110/247 (44.53%), Query Frame = 0

Query: 11  NDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVEKSRKMLEETLKWRAA 70
           N + ++E K+ EL+  L  L+        DA L R+L AR ++V  +++M E   KWR  
Sbjct: 27  NLTAEQEQKLGELKMIL--LTKGYEDRTDDATLLRFLRARKFDVPLAQEMWENCEKWRKE 86

Query: 71  YKPEEIGWDEVAFEGETGKVSRANFY---DRHGRSVLIMRPG------MQNTTPSEASVR 130
           +    I  D    E +        +Y   D+ GR V +   G      M   T  E  +R
Sbjct: 87  FGTNTILEDFWYKEKKEVAKLYPQYYHKTDKDGRPVYVENVGKVNIHEMYKITTQERMLR 146

Query: 131 HLVYLLENAIMN--------LGEGQEQMCWLIDFTGFTMKTNVSV-KIAADIINVLQNHY 190
           +LV+  E+ + +        +G   E  C ++D  G ++ +   V     D  N+ QN+Y
Sbjct: 147 NLVWEYESFVRHRLPACSRVVGHLIETSCTILDLKGVSLSSASQVYGFLKDASNIGQNYY 206

Query: 191 PERLAFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPS 240
           PER+   +L N P  F   +  IK FLDP T  K+       K   E + +     NLP 
Sbjct: 207 PERMGKFYLINAPFGFSTVFSVIKRFLDPVTVSKIHVYGSNYK---EKLLAQVPAYNLPI 266

BLAST of CSPI04G04940 vs. ExPASy TrEMBL
Match: A0A0A0KWJ1 (CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G038670 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 2.7e-166
Identity = 290/290 (100.00%), Postives = 290/290 (100.00%), Query Frame = 0

Query: 1   MFRSKKHSQNNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVEKSRKM 60
           MFRSKKHSQNNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVEKSRKM
Sbjct: 1   MFRSKKHSQNNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVEKSRKM 60

Query: 61  LEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTPSEASV 120
           LEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTPSEASV
Sbjct: 61  LEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTPSEASV 120

Query: 121 RHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPERLAFAF 180
           RHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPERLAFAF
Sbjct: 121 RHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPERLAFAF 180

Query: 181 LYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFGGKATL 240
           LYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFGGKATL
Sbjct: 181 LYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFGGKATL 240

Query: 241 EYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEPVTSTN 291
           EYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEPVTSTN
Sbjct: 241 EYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEPVTSTN 290

BLAST of CSPI04G04940 vs. ExPASy TrEMBL
Match: A0A5A7TRI3 (Random slug protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G005530 PE=4 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.3e-160
Identity = 284/296 (95.95%), Postives = 286/296 (96.62%), Query Frame = 0

Query: 1   MFRSKKHS------QNNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNV 60
           MFRSKKHS      Q+NDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNV
Sbjct: 28  MFRSKKHSQSHNNGQDNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNV 87

Query: 61  EKSRKMLEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTT 120
           EKSRKMLEETLKWRAAYKPEEI WDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTT
Sbjct: 88  EKSRKMLEETLKWRAAYKPEEIRWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTT 147

Query: 121 PSEASVRHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPE 180
           PSEASVRHLVYLLENAI+NLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPE
Sbjct: 148 PSEASVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPE 207

Query: 181 RLAFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVF 240
           RLAFAFLYNPPKFFQAFWKAIKYFLDPKT QKVKFVNPKDKGSVELMKSHFDMENLPSVF
Sbjct: 208 RLAFAFLYNPPKFFQAFWKAIKYFLDPKTSQKVKFVNPKDKGSVELMKSHFDMENLPSVF 267

Query: 241 GGKATLEYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEPVTSTN 291
           GGKATLEYDHQQFSQMMGQDELKAAQFWGFDEKA HSVNG SSGPEVGPEPVTSTN
Sbjct: 268 GGKATLEYDHQQFSQMMGQDELKAAQFWGFDEKAHHSVNGHSSGPEVGPEPVTSTN 323

BLAST of CSPI04G04940 vs. ExPASy TrEMBL
Match: A0A1S3BX30 (random slug protein 5 OS=Cucumis melo OX=3656 GN=LOC103494385 PE=4 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.3e-160
Identity = 284/296 (95.95%), Postives = 286/296 (96.62%), Query Frame = 0

Query: 1   MFRSKKHS------QNNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNV 60
           MFRSKKHS      Q+NDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNV
Sbjct: 1   MFRSKKHSQSHNNGQDNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNV 60

Query: 61  EKSRKMLEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTT 120
           EKSRKMLEETLKWRAAYKPEEI WDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTT
Sbjct: 61  EKSRKMLEETLKWRAAYKPEEIRWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTT 120

Query: 121 PSEASVRHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPE 180
           PSEASVRHLVYLLENAI+NLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPE
Sbjct: 121 PSEASVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPE 180

Query: 181 RLAFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVF 240
           RLAFAFLYNPPKFFQAFWKAIKYFLDPKT QKVKFVNPKDKGSVELMKSHFDMENLPSVF
Sbjct: 181 RLAFAFLYNPPKFFQAFWKAIKYFLDPKTSQKVKFVNPKDKGSVELMKSHFDMENLPSVF 240

Query: 241 GGKATLEYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEPVTSTN 291
           GGKATLEYDHQQFSQMMGQDELKAAQFWGFDEKA HSVNG SSGPEVGPEPVTSTN
Sbjct: 241 GGKATLEYDHQQFSQMMGQDELKAAQFWGFDEKAHHSVNGHSSGPEVGPEPVTSTN 296

BLAST of CSPI04G04940 vs. ExPASy TrEMBL
Match: A0A6J1ESC3 (phosphatidylinositol transfer protein 3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436058 PE=4 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 5.6e-148
Identity = 263/295 (89.15%), Postives = 273/295 (92.54%), Query Frame = 0

Query: 1   MFRSKKHSQ-----NNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVE 60
           MFRSKKHSQ     NNDSTQE+GKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVE
Sbjct: 1   MFRSKKHSQSHNHHNNDSTQEDGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVE 60

Query: 61  KSRKMLEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTP 120
           K+RKMLEETLKWRAAYKPEEI W EVAFEGETGKVSRA+F DR GRSVLIMRPGMQNTTP
Sbjct: 61  KARKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRADFCDRQGRSVLIMRPGMQNTTP 120

Query: 121 SEASVRHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPER 180
           SE SVRHLVYLLENAI+NL EGQEQMCWLIDFTGFTMKTNVSVKIA+D+IN+LQNHYPER
Sbjct: 121 SEVSVRHLVYLLENAILNLVEGQEQMCWLIDFTGFTMKTNVSVKIASDVINILQNHYPER 180

Query: 181 LAFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFG 240
           LA AFLYNPPK FQAFWKAIKYFLDPKT QKVKFVNPKDKGSVELMKS+FDMENLPSVFG
Sbjct: 181 LAVAFLYNPPKIFQAFWKAIKYFLDPKTSQKVKFVNPKDKGSVELMKSYFDMENLPSVFG 240

Query: 241 GKATLEYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEPVTSTN 291
           GKATLEYDHQQFSQMM QDELKAA  WG DEKA H  NGPSSGPEV PEP+T+TN
Sbjct: 241 GKATLEYDHQQFSQMMAQDELKAALVWGLDEKAHHITNGPSSGPEVAPEPITATN 295

BLAST of CSPI04G04940 vs. ExPASy TrEMBL
Match: A0A6J1J6E4 (phosphatidylinositol transfer protein 3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482999 PE=4 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 8.9e-146
Identity = 260/295 (88.14%), Postives = 270/295 (91.53%), Query Frame = 0

Query: 1   MFRSKKHSQ-----NNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVE 60
           MFR KKHSQ     +NDSTQE+GKI ELRAALGPLS RSSKYCTDACLRRYLIARNWNVE
Sbjct: 1   MFRLKKHSQSHNHHDNDSTQEDGKIIELRAALGPLSRRSSKYCTDACLRRYLIARNWNVE 60

Query: 61  KSRKMLEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTP 120
           K+RKMLEETLKWRAAYKPEEI W EVAFEGETGKVSRANF DR GRSVLIMRPGMQNTTP
Sbjct: 61  KARKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFCDRQGRSVLIMRPGMQNTTP 120

Query: 121 SEASVRHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPER 180
           SE SVRHLVYLLENAI+NL EGQEQMCWLIDFTGFTMKTNVSVKIA+D+IN+LQNHYPER
Sbjct: 121 SEVSVRHLVYLLENAILNLVEGQEQMCWLIDFTGFTMKTNVSVKIASDVINILQNHYPER 180

Query: 181 LAFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFG 240
           LA AFLYNPPK FQAFWKAIKYFLDPKT QKVKFVNPKDKGSVELMKS+FDMENLPSVFG
Sbjct: 181 LAVAFLYNPPKIFQAFWKAIKYFLDPKTSQKVKFVNPKDKGSVELMKSYFDMENLPSVFG 240

Query: 241 GKATLEYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEPVTSTN 291
           GKATLEYDHQQFSQMM QDELKAAQ W  DEKA H  NGPSSGPEV PEP+T+TN
Sbjct: 241 GKATLEYDHQQFSQMMAQDELKAAQVWALDEKAHHITNGPSSGPEVAPEPITATN 295

BLAST of CSPI04G04940 vs. NCBI nr
Match: XP_004149151.1 (phosphatidylinositol transfer protein 3 [Cucumis sativus] >KGN53249.1 hypothetical protein Csa_014408 [Cucumis sativus])

HSP 1 Score: 594.3 bits (1531), Expect = 5.5e-166
Identity = 290/290 (100.00%), Postives = 290/290 (100.00%), Query Frame = 0

Query: 1   MFRSKKHSQNNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVEKSRKM 60
           MFRSKKHSQNNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVEKSRKM
Sbjct: 1   MFRSKKHSQNNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVEKSRKM 60

Query: 61  LEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTPSEASV 120
           LEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTPSEASV
Sbjct: 61  LEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTPSEASV 120

Query: 121 RHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPERLAFAF 180
           RHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPERLAFAF
Sbjct: 121 RHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPERLAFAF 180

Query: 181 LYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFGGKATL 240
           LYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFGGKATL
Sbjct: 181 LYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFGGKATL 240

Query: 241 EYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEPVTSTN 291
           EYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEPVTSTN
Sbjct: 241 EYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEPVTSTN 290

BLAST of CSPI04G04940 vs. NCBI nr
Match: KAA0044746.1 (random slug protein 5 [Cucumis melo var. makuwa] >TYK16716.1 random slug protein 5 [Cucumis melo var. makuwa])

HSP 1 Score: 573.2 bits (1476), Expect = 1.3e-159
Identity = 284/296 (95.95%), Postives = 286/296 (96.62%), Query Frame = 0

Query: 1   MFRSKKHS------QNNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNV 60
           MFRSKKHS      Q+NDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNV
Sbjct: 28  MFRSKKHSQSHNNGQDNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNV 87

Query: 61  EKSRKMLEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTT 120
           EKSRKMLEETLKWRAAYKPEEI WDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTT
Sbjct: 88  EKSRKMLEETLKWRAAYKPEEIRWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTT 147

Query: 121 PSEASVRHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPE 180
           PSEASVRHLVYLLENAI+NLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPE
Sbjct: 148 PSEASVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPE 207

Query: 181 RLAFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVF 240
           RLAFAFLYNPPKFFQAFWKAIKYFLDPKT QKVKFVNPKDKGSVELMKSHFDMENLPSVF
Sbjct: 208 RLAFAFLYNPPKFFQAFWKAIKYFLDPKTSQKVKFVNPKDKGSVELMKSHFDMENLPSVF 267

Query: 241 GGKATLEYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEPVTSTN 291
           GGKATLEYDHQQFSQMMGQDELKAAQFWGFDEKA HSVNG SSGPEVGPEPVTSTN
Sbjct: 268 GGKATLEYDHQQFSQMMGQDELKAAQFWGFDEKAHHSVNGHSSGPEVGPEPVTSTN 323

BLAST of CSPI04G04940 vs. NCBI nr
Match: XP_008453740.1 (PREDICTED: random slug protein 5 [Cucumis melo])

HSP 1 Score: 573.2 bits (1476), Expect = 1.3e-159
Identity = 284/296 (95.95%), Postives = 286/296 (96.62%), Query Frame = 0

Query: 1   MFRSKKHS------QNNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNV 60
           MFRSKKHS      Q+NDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNV
Sbjct: 1   MFRSKKHSQSHNNGQDNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNV 60

Query: 61  EKSRKMLEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTT 120
           EKSRKMLEETLKWRAAYKPEEI WDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTT
Sbjct: 61  EKSRKMLEETLKWRAAYKPEEIRWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTT 120

Query: 121 PSEASVRHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPE 180
           PSEASVRHLVYLLENAI+NLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPE
Sbjct: 121 PSEASVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPE 180

Query: 181 RLAFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVF 240
           RLAFAFLYNPPKFFQAFWKAIKYFLDPKT QKVKFVNPKDKGSVELMKSHFDMENLPSVF
Sbjct: 181 RLAFAFLYNPPKFFQAFWKAIKYFLDPKTSQKVKFVNPKDKGSVELMKSHFDMENLPSVF 240

Query: 241 GGKATLEYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEPVTSTN 291
           GGKATLEYDHQQFSQMMGQDELKAAQFWGFDEKA HSVNG SSGPEVGPEPVTSTN
Sbjct: 241 GGKATLEYDHQQFSQMMGQDELKAAQFWGFDEKAHHSVNGHSSGPEVGPEPVTSTN 296

BLAST of CSPI04G04940 vs. NCBI nr
Match: XP_038893006.1 (phosphatidylinositol transfer protein 3-like [Benincasa hispida] >XP_038893012.1 phosphatidylinositol transfer protein 3-like [Benincasa hispida])

HSP 1 Score: 547.0 bits (1408), Expect = 1.0e-151
Identity = 270/295 (91.53%), Postives = 278/295 (94.24%), Query Frame = 0

Query: 1   MFRSKKHSQNN-----DSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVE 60
           MFR KKHSQ++     DSTQE+GKINELRAALGPLSSR+ KYCTDACLRRYLIARNWNVE
Sbjct: 1   MFRLKKHSQSHNRQDIDSTQEDGKINELRAALGPLSSRNLKYCTDACLRRYLIARNWNVE 60

Query: 61  KSRKMLEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTP 120
           KSRKMLEETLKWRAA+KPEEI W EVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTP
Sbjct: 61  KSRKMLEETLKWRAAFKPEEIRWHEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTP 120

Query: 121 SEASVRHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPER 180
           SEASVRHLVYLLENAIMNLGEGQEQMCWLIDF+GFTMKTN+SVKIA DIINVLQNHYPER
Sbjct: 121 SEASVRHLVYLLENAIMNLGEGQEQMCWLIDFSGFTMKTNISVKIAGDIINVLQNHYPER 180

Query: 181 LAFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFG 240
           LAFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFG
Sbjct: 181 LAFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFG 240

Query: 241 GKATLEYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEPVTSTN 291
           GKATLEYDHQQFSQMM QDELKAAQFWG DE   H VNGPSSGPEV PEP+TSTN
Sbjct: 241 GKATLEYDHQQFSQMMAQDELKAAQFWGLDEN-HHIVNGPSSGPEVAPEPITSTN 294

BLAST of CSPI04G04940 vs. NCBI nr
Match: XP_023553217.1 (phosphatidylinositol transfer protein 3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023553218.1 phosphatidylinositol transfer protein 3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023553219.1 phosphatidylinositol transfer protein 3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023553220.1 phosphatidylinositol transfer protein 3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023553221.1 phosphatidylinositol transfer protein 3-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 536.2 bits (1380), Expect = 1.8e-148
Identity = 264/295 (89.49%), Postives = 274/295 (92.88%), Query Frame = 0

Query: 1   MFRSKKHSQ-----NNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVE 60
           MFRSKKHSQ     +NDSTQE+GKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVE
Sbjct: 1   MFRSKKHSQSHNHRDNDSTQEDGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVE 60

Query: 61  KSRKMLEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTP 120
           K+RKMLEETLKWRAAYKPEEI W EVAFEGETGKVSRANF DR GRSVLIMRPGMQNTTP
Sbjct: 61  KARKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFCDRQGRSVLIMRPGMQNTTP 120

Query: 121 SEASVRHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPER 180
           SE SVRHLVYLLENAI+NL EGQEQMCWLIDFTGFTMKTNVSVKIA+D+IN+LQNHYPER
Sbjct: 121 SEVSVRHLVYLLENAILNLVEGQEQMCWLIDFTGFTMKTNVSVKIASDVINILQNHYPER 180

Query: 181 LAFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFG 240
           LA AFLYNPPK FQAFWKAIKYFLDPKT QKVKFVNPKDKGSVELMKS+FDMENLPSVFG
Sbjct: 181 LAVAFLYNPPKIFQAFWKAIKYFLDPKTSQKVKFVNPKDKGSVELMKSYFDMENLPSVFG 240

Query: 241 GKATLEYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEPVTSTN 291
           GKATLEYDHQQFSQMM QDELKAAQ WG DEKA H  NGPSSGPEV PEP+T+TN
Sbjct: 241 GKATLEYDHQQFSQMMAQDELKAAQVWGLDEKAHHITNGPSSGPEVAPEPITATN 295

BLAST of CSPI04G04940 vs. TAIR 10
Match: AT1G75170.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 365.2 bits (936), Expect = 5.0e-101
Identity = 177/293 (60.41%), Postives = 225/293 (76.79%), Query Frame = 0

Query: 1   MFRSKKHSQNNDSTQE----EGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVEK 60
           MFR K  SQ     +E    E K+ EL+  +G LS R+S YC+DACL+RYL ARNWNV K
Sbjct: 1   MFRWKNSSQTEQEQKEAALREAKMKELKTLIGQLSGRNSLYCSDACLKRYLEARNWNVGK 60

Query: 61  SRKMLEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTPS 120
           ++KMLEETLKWR+++KPEEI W+EV+ EGETGKV +A F+DRHGR+VLI+RPG+QNT   
Sbjct: 61  AKKMLEETLKWRSSFKPEEIRWNEVSGEGETGKVYKAGFHDRHGRTVLILRPGLQNTKSL 120

Query: 121 EASVRHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPERL 180
           E  ++HLVYL+ENAI+NL E QEQM WLIDFTG++M T+V +K A + IN+LQNHYPERL
Sbjct: 121 ENQMKHLVYLIENAILNLPEDQEQMSWLIDFTGWSMSTSVPIKSARETINILQNHYPERL 180

Query: 181 AFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFGG 240
           A AFLYNPP+ F+AFWK +KYF+D KTF KVKFV PK+  SVELM + FD ENLP+ FGG
Sbjct: 181 AVAFLYNPPRLFEAFWKIVKYFIDAKTFVKVKFVYPKNSESVELMSTFFDEENLPTEFGG 240

Query: 241 KATLEYDHQQFSQMMGQDELKAAQFWGF---DEKAQHSVNGPSSGPEVGPEPV 287
           KA L+Y++++FS+ M QD++K A FWG    +    H+ NG  SG E+ PEP+
Sbjct: 241 KALLQYNYEEFSKQMNQDDVKTANFWGLGHSNNNQLHASNG-FSGAEIAPEPI 292

BLAST of CSPI04G04940 vs. TAIR 10
Match: AT1G75170.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 365.2 bits (936), Expect = 5.0e-101
Identity = 177/293 (60.41%), Postives = 225/293 (76.79%), Query Frame = 0

Query: 1   MFRSKKHSQNNDSTQE----EGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVEK 60
           MFR K  SQ     +E    E K+ EL+  +G LS R+S YC+DACL+RYL ARNWNV K
Sbjct: 1   MFRWKNSSQTEQEQKEAALREAKMKELKTLIGQLSGRNSLYCSDACLKRYLEARNWNVGK 60

Query: 61  SRKMLEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTPS 120
           ++KMLEETLKWR+++KPEEI W+EV+ EGETGKV +A F+DRHGR+VLI+RPG+QNT   
Sbjct: 61  AKKMLEETLKWRSSFKPEEIRWNEVSGEGETGKVYKAGFHDRHGRTVLILRPGLQNTKSL 120

Query: 121 EASVRHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPERL 180
           E  ++HLVYL+ENAI+NL E QEQM WLIDFTG++M T+V +K A + IN+LQNHYPERL
Sbjct: 121 ENQMKHLVYLIENAILNLPEDQEQMSWLIDFTGWSMSTSVPIKSARETINILQNHYPERL 180

Query: 181 AFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFGG 240
           A AFLYNPP+ F+AFWK +KYF+D KTF KVKFV PK+  SVELM + FD ENLP+ FGG
Sbjct: 181 AVAFLYNPPRLFEAFWKIVKYFIDAKTFVKVKFVYPKNSESVELMSTFFDEENLPTEFGG 240

Query: 241 KATLEYDHQQFSQMMGQDELKAAQFWGF---DEKAQHSVNGPSSGPEVGPEPV 287
           KA L+Y++++FS+ M QD++K A FWG    +    H+ NG  SG E+ PEP+
Sbjct: 241 KALLQYNYEEFSKQMNQDDVKTANFWGLGHSNNNQLHASNG-FSGAEIAPEPI 292

BLAST of CSPI04G04940 vs. TAIR 10
Match: AT4G36640.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 363.2 bits (931), Expect = 1.9e-100
Identity = 173/286 (60.49%), Postives = 225/286 (78.67%), Query Frame = 0

Query: 1   MFRSKK-HSQNNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVEKSRK 60
           MFR +  H  +ND +Q++ K+ EL++A+GPLS  S  +C+DA LRR+L ARNW+VEK++K
Sbjct: 1   MFRRRNAHQLDNDDSQQDNKVRELKSAIGPLSGHSLVFCSDASLRRFLDARNWDVEKAKK 60

Query: 61  MLEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTPSEAS 120
           M++ETLKWR+ YKP+EI W++VA EGETGK SRA+F+DR GR VLIMRP MQN+T  E +
Sbjct: 61  MIQETLKWRSTYKPQEIRWNQVAHEGETGKASRASFHDRQGRVVLIMRPAMQNSTSQEGN 120

Query: 121 VRHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPERLAFA 180
           +RHLVYLLENAI+NL +GQ+QM WLIDFTG++M  N  +K   +II++LQN+YPERL  A
Sbjct: 121 IRHLVYLLENAIINLPKGQKQMSWLIDFTGWSMAVNPPMKTTREIIHILQNYYPERLGIA 180

Query: 181 FLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFGGKAT 240
           FLYNPP+ FQA ++A KYFLDP+T +KVKFV PKDK S ELM +HFD+ENLP  FGG+AT
Sbjct: 181 FLYNPPRLFQAVYRAAKYFLDPRTAEKVKFVYPKDKASDELMTTHFDVENLPKEFGGEAT 240

Query: 241 LEYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEP 286
           LEYDH+ FS+ M +D+LK A++WG + K     NG  S  +V PEP
Sbjct: 241 LEYDHEDFSRQMYEDDLKTAKYWGLEGKHYPKTNG-FSPSDVVPEP 285

BLAST of CSPI04G04940 vs. TAIR 10
Match: AT4G36640.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 300.4 bits (768), Expect = 1.5e-81
Identity = 142/226 (62.83%), Postives = 179/226 (79.20%), Query Frame = 0

Query: 60  MLEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTPSEAS 119
           M++ETLKWR+ YKP+EI W++VA EGETGK SRA+F+DR GR VLIMRP MQN+T  E +
Sbjct: 1   MIQETLKWRSTYKPQEIRWNQVAHEGETGKASRASFHDRQGRVVLIMRPAMQNSTSQEGN 60

Query: 120 VRHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPERLAFA 179
           +RHLVYLLENAI+NL +GQ+QM WLIDFTG++M  N  +K   +II++LQN+YPERL  A
Sbjct: 61  IRHLVYLLENAIINLPKGQKQMSWLIDFTGWSMAVNPPMKTTREIIHILQNYYPERLGIA 120

Query: 180 FLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFGGKAT 239
           FLYNPP+ FQA ++A KYFLDP+T +KVKFV PKDK S ELM +HFD+ENLP  FGG+AT
Sbjct: 121 FLYNPPRLFQAVYRAAKYFLDPRTAEKVKFVYPKDKASDELMTTHFDVENLPKEFGGEAT 180

Query: 240 LEYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEP 286
           LEYDH+ FS+ M +D+LK A++WG + K     NG  S  +V PEP
Sbjct: 181 LEYDHEDFSRQMYEDDLKTAKYWGLEGKHYPKTNG-FSPSDVVPEP 225

BLAST of CSPI04G04940 vs. TAIR 10
Match: AT1G75170.3 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 269.2 bits (687), Expect = 3.8e-72
Identity = 128/209 (61.24%), Postives = 163/209 (77.99%), Query Frame = 0

Query: 81  VAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTPSEASVRHLVYLLENAIMNLGEGQEQ 140
           V+ EGETGKV +A F+DRHGR+VLI+RPG+QNT   E  ++HLVYL+ENAI+NL E QEQ
Sbjct: 2   VSGEGETGKVYKAGFHDRHGRTVLILRPGLQNTKSLENQMKHLVYLIENAILNLPEDQEQ 61

Query: 141 MCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPERLAFAFLYNPPKFFQAFWKAIKYFLD 200
           M WLIDFTG++M T+V +K A + IN+LQNHYPERLA AFLYNPP+ F+AFWK +KYF+D
Sbjct: 62  MSWLIDFTGWSMSTSVPIKSARETINILQNHYPERLAVAFLYNPPRLFEAFWKIVKYFID 121

Query: 201 PKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFGGKATLEYDHQQFSQMMGQDELKAAQ 260
            KTF KVKFV PK+  SVELM + FD ENLP+ FGGKA L+Y++++FS+ M QD++K A 
Sbjct: 122 AKTFVKVKFVYPKNSESVELMSTFFDEENLPTEFGGKALLQYNYEEFSKQMNQDDVKTAN 181

Query: 261 FWGF---DEKAQHSVNGPSSGPEVGPEPV 287
           FWG    +    H+ NG  SG E+ PEP+
Sbjct: 182 FWGLGHSNNNQLHASNG-FSGAEIAPEPI 209

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9UU991.3e-2934.78CRAL-TRIO domain-containing protein C23B6.04c OS=Schizosaccharomyces pombe (stra... [more]
Q55CU88.0e-2733.79Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=... [more]
P538602.2e-2430.74Phosphatidylinositol transfer protein PDR16 OS=Saccharomyces cerevisiae (strain ... [more]
P538445.4e-2331.39Phosphatidylinositol transfer protein PDR17 OS=Saccharomyces cerevisiae (strain ... [more]
P458164.6e-1428.34SEC14 cytosolic factor OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=28459... [more]
Match NameE-valueIdentityDescription
A0A0A0KWJ12.7e-166100.00CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G038670 P... [more]
A0A5A7TRI36.3e-16095.95Random slug protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21... [more]
A0A1S3BX306.3e-16095.95random slug protein 5 OS=Cucumis melo OX=3656 GN=LOC103494385 PE=4 SV=1[more]
A0A6J1ESC35.6e-14889.15phosphatidylinositol transfer protein 3-like isoform X1 OS=Cucurbita moschata OX... [more]
A0A6J1J6E48.9e-14688.14phosphatidylinositol transfer protein 3-like isoform X1 OS=Cucurbita maxima OX=3... [more]
Match NameE-valueIdentityDescription
XP_004149151.15.5e-166100.00phosphatidylinositol transfer protein 3 [Cucumis sativus] >KGN53249.1 hypothetic... [more]
KAA0044746.11.3e-15995.95random slug protein 5 [Cucumis melo var. makuwa] >TYK16716.1 random slug protein... [more]
XP_008453740.11.3e-15995.95PREDICTED: random slug protein 5 [Cucumis melo][more]
XP_038893006.11.0e-15191.53phosphatidylinositol transfer protein 3-like [Benincasa hispida] >XP_038893012.1... [more]
XP_023553217.11.8e-14889.49phosphatidylinositol transfer protein 3-like isoform X1 [Cucurbita pepo subsp. p... [more]
Match NameE-valueIdentityDescription
AT1G75170.15.0e-10160.41Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G75170.25.0e-10160.41Sec14p-like phosphatidylinositol transfer family protein [more]
AT4G36640.11.9e-10060.49Sec14p-like phosphatidylinositol transfer family protein [more]
AT4G36640.21.5e-8162.83Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G75170.33.8e-7261.24Sec14p-like phosphatidylinositol transfer family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001251CRAL-TRIO lipid binding domainSMARTSM00516sec14_4coord: 84..239
e-value: 1.1E-46
score: 171.0
IPR001251CRAL-TRIO lipid binding domainPFAMPF00650CRAL_TRIOcoord: 96..237
e-value: 1.8E-35
score: 122.0
IPR001251CRAL-TRIO lipid binding domainPROSITEPS50191CRAL_TRIOcoord: 78..242
score: 21.075546
IPR001251CRAL-TRIO lipid binding domainCDDcd00170SEC14coord: 97..237
e-value: 1.71969E-39
score: 133.665
IPR011074CRAL/TRIO, N-terminal domainSMARTSM01100CRAL_TRIO_N_2coord: 39..64
e-value: 1.1E-7
score: 41.6
IPR011074CRAL/TRIO, N-terminal domainPFAMPF03765CRAL_TRIO_Ncoord: 38..63
e-value: 1.2E-6
score: 28.6
IPR036865CRAL-TRIO lipid binding domain superfamilyGENE3D3.40.525.10coord: 2..272
e-value: 2.2E-81
score: 275.4
IPR036865CRAL-TRIO lipid binding domain superfamilySUPERFAMILY52087CRAL/TRIO domaincoord: 78..248
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 268..290
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 270..290
NoneNo IPR availablePANTHERPTHR45824:SF17DIVERGENT CRAL/TRIO DOMAIN PROTEINcoord: 5..287
NoneNo IPR availablePANTHERPTHR45824GH16843Pcoord: 5..287
IPR036273CRAL/TRIO, N-terminal domain superfamilySUPERFAMILY46938CRAL/TRIO N-terminal domaincoord: 8..80

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G04940.1CSPI04G04940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0120009 intermembrane lipid transfer
biological_process GO:0015914 phospholipid transport
cellular_component GO:0016020 membrane
molecular_function GO:0008526 phosphatidylinositol transfer activity