CSPI04G04850 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G04850
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionMitochondrial transcription termination factor family protein
LocationChr4: 3226669 .. 3228216 (-)
RNA-Seq ExpressionCSPI04G04850
SyntenyCSPI04G04850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGGGCTAAATTTGTAATTTAAACTTTTAGAAAAGTTGAATCGGAGAAGATGAGGGATGGAAGCGACGGAGAGAAGAAAAACGGGAGAAGCTTTTGAGAAACGCCGCCTCCTTAAACCCTAAAATTTCTATGCTTTTCAGCTCTTAAATTAAATGTACGCTATGTATCCATCATTCTCTGTTTGTGTTCCCTCTCCTCTTAATCCTCCTTTCTTCCTCCGACTAATTCATCCATTCTTGTTTTCTTTCTTCTCTTCTTCTTCTTCTTCTTCTTCTTCCCACTCCCAACCACCCTCTCATTCCTATGGAATCGTCCTCCAATACCTCATCGATACCTTCCAACTCTCCCCCGCCAGAGCCGTCTCCATTATGGCTACCCGTAGAGGCATTCAATCAACTGAAAAGCCTCGTTCCGTTTACAAATATCTTTCGGACCTCGGATTATCCGACACCCAAATTAAATCAGCCGTTCGAATCACACCCCAAATCGCGTTTTCCAGCATTGAGAAAACACTGAAGCCAAAGATCGAGTTCTTACAGAATCTTGGCTTTGTTGGCTCAGATTTGAGTAAGTTCATTTCCAGGCAATCTAAATTTTTTTCTTCTAGTTTGGAAAAGACATTGATGCCCAATGTTGAGATTCTTAAGAATGTTTTGCCCAAGGGTACGTGTAATGACGATCTCCCGAAAGTTTTGCGGCGATGTTCTGATGTACTAACGAGATCCCCGTATAAAGTGTTGTCTGTAAATATCAATTACTTACGAAGTTGTGGGATTGTTGACTATCAACTCTCTACGTTATTGAAGCGACAACCTGCACTTTTTATTATGCATGAATCTCGACTTAAAGATTTTGTTTCAATGGCTGTAAAGGCTGGTTTTTCTCCCAACGGTACAATGTTTATTCATGGACTTCATTCTATCAGTAGTATAAGTAATGCAACTTATAAGAAGAAAGTGAAGCTGATTTGTAGTTTTGGAATAACTGAGAAAGAATGTATGAGAATGTTTAATTCGGCTCCTGTTTTGATGAGGACTTCCGTTGGTAAACTTGAGGTTGGTCTAGAATTCTTCATGAATGAGGCAAAAGTTAGCAAATCAGATATTGTTCGTAATCCTTTTTGTTTGATGCACGCAATGCATGGAAGGGTGCTCCCTCGTTATAGAGTTCTAGAGGTTTTGAAGTCGAAGAGGCTAACGAAGAAGCTCCCGAAGTTGATCGATTCATTGTGGATGCCTGATGAGGATTTCTTAGATAAATTCGTTCGTAGGTTTCCTGACAATATGAATGATTTGTTTGAGGCCTTTAGAGGTAATTCGAGGAGATAGCAACATGAAGATGGGGCAAGAACTAGAGCTAGTTTGATCTAACATTTGGTCAATACCTTGAGAACGAGTGGTCGGGGTTGGAAGGTGTTTTGATCATAGTGAGCTCTTGATTCCTCCTTTTCATGTTAGGATGGTCTCTCTCTATCCCACATTGTATGTGCTACTATGGTGAATGTACTTGGATTAAAGCTTCACATTACAAAACACAACTATTTTGCAT

mRNA sequence

TGGGGCTAAATTTGTAATTTAAACTTTTAGAAAAGTTGAATCGGAGAAGATGAGGGATGGAAGCGACGGAGAGAAGAAAAACGGGAGAAGCTTTTGAGAAACGCCGCCTCCTTAAACCCTAAAATTTCTATGCTTTTCAGCTCTTAAATTAAATGTACGCTATGTATCCATCATTCTCTGTTTGTGTTCCCTCTCCTCTTAATCCTCCTTTCTTCCTCCGACTAATTCATCCATTCTTGTTTTCTTTCTTCTCTTCTTCTTCTTCTTCTTCTTCTTCCCACTCCCAACCACCCTCTCATTCCTATGGAATCGTCCTCCAATACCTCATCGATACCTTCCAACTCTCCCCCGCCAGAGCCGTCTCCATTATGGCTACCCGTAGAGGCATTCAATCAACTGAAAAGCCTCGTTCCGTTTACAAATATCTTTCGGACCTCGGATTATCCGACACCCAAATTAAATCAGCCGTTCGAATCACACCCCAAATCGCGTTTTCCAGCATTGAGAAAACACTGAAGCCAAAGATCGAGTTCTTACAGAATCTTGGCTTTGTTGGCTCAGATTTGAGTAAGTTCATTTCCAGGCAATCTAAATTTTTTTCTTCTAGTTTGGAAAAGACATTGATGCCCAATGTTGAGATTCTTAAGAATGTTTTGCCCAAGGGTACGTGTAATGACGATCTCCCGAAAGTTTTGCGGCGATGTTCTGATGTACTAACGAGATCCCCGTATAAAGTGTTGTCTGTAAATATCAATTACTTACGAAGTTGTGGGATTGTTGACTATCAACTCTCTACGTTATTGAAGCGACAACCTGCACTTTTTATTATGCATGAATCTCGACTTAAAGATTTTGTTTCAATGGCTGTAAAGGCTGGTTTTTCTCCCAACGGTACAATGTTTATTCATGGACTTCATTCTATCAGTAGTATAAGTAATGCAACTTATAAGAAGAAAGTGAAGCTGATTTGTAGTTTTGGAATAACTGAGAAAGAATGTATGAGAATGTTTAATTCGGCTCCTGTTTTGATGAGGACTTCCGTTGGTAAACTTGAGGTTGGTCTAGAATTCTTCATGAATGAGGCAAAAGTTAGCAAATCAGATATTGTTCGTAATCCTTTTTGTTTGATGCACGCAATGCATGGAAGGGTGCTCCCTCGTTATAGAGTTCTAGAGGTTTTGAAGTCGAAGAGGCTAACGAAGAAGCTCCCGAAGTTGATCGATTCATTGTGGATGCCTGATGAGGATTTCTTAGATAAATTCGTTCGTAGGTTTCCTGACAATATGAATGATTTGTTTGAGGCCTTTAGAGGTAATTCGAGGAGATAGCAACATGAAGATGGGGCAAGAACTAGAGCTAGTTTGATCTAACATTTGGTCAATACCTTGAGAACGAGTGGTCGGGGTTGGAAGGTGTTTTGATCATAGTGAGCTCTTGATTCCTCCTTTTCATGTTAGGATGGTCTCTCTCTATCCCACATTGTATGTGCTACTATGGTGAATGTACTTGGATTAAAGCTTCACATTACAAAACACAACTATTTTGCAT

Coding sequence (CDS)

ATGTACGCTATGTATCCATCATTCTCTGTTTGTGTTCCCTCTCCTCTTAATCCTCCTTTCTTCCTCCGACTAATTCATCCATTCTTGTTTTCTTTCTTCTCTTCTTCTTCTTCTTCTTCTTCTTCCCACTCCCAACCACCCTCTCATTCCTATGGAATCGTCCTCCAATACCTCATCGATACCTTCCAACTCTCCCCCGCCAGAGCCGTCTCCATTATGGCTACCCGTAGAGGCATTCAATCAACTGAAAAGCCTCGTTCCGTTTACAAATATCTTTCGGACCTCGGATTATCCGACACCCAAATTAAATCAGCCGTTCGAATCACACCCCAAATCGCGTTTTCCAGCATTGAGAAAACACTGAAGCCAAAGATCGAGTTCTTACAGAATCTTGGCTTTGTTGGCTCAGATTTGAGTAAGTTCATTTCCAGGCAATCTAAATTTTTTTCTTCTAGTTTGGAAAAGACATTGATGCCCAATGTTGAGATTCTTAAGAATGTTTTGCCCAAGGGTACGTGTAATGACGATCTCCCGAAAGTTTTGCGGCGATGTTCTGATGTACTAACGAGATCCCCGTATAAAGTGTTGTCTGTAAATATCAATTACTTACGAAGTTGTGGGATTGTTGACTATCAACTCTCTACGTTATTGAAGCGACAACCTGCACTTTTTATTATGCATGAATCTCGACTTAAAGATTTTGTTTCAATGGCTGTAAAGGCTGGTTTTTCTCCCAACGGTACAATGTTTATTCATGGACTTCATTCTATCAGTAGTATAAGTAATGCAACTTATAAGAAGAAAGTGAAGCTGATTTGTAGTTTTGGAATAACTGAGAAAGAATGTATGAGAATGTTTAATTCGGCTCCTGTTTTGATGAGGACTTCCGTTGGTAAACTTGAGGTTGGTCTAGAATTCTTCATGAATGAGGCAAAAGTTAGCAAATCAGATATTGTTCGTAATCCTTTTTGTTTGATGCACGCAATGCATGGAAGGGTGCTCCCTCGTTATAGAGTTCTAGAGGTTTTGAAGTCGAAGAGGCTAACGAAGAAGCTCCCGAAGTTGATCGATTCATTGTGGATGCCTGATGAGGATTTCTTAGATAAATTCGTTCGTAGGTTTCCTGACAATATGAATGATTTGTTTGAGGCCTTTAGAGGTAATTCGAGGAGATAG

Protein sequence

MYAMYPSFSVCVPSPLNPPFFLRLIHPFLFSFFSSSSSSSSSHSQPPSHSYGIVLQYLIDTFQLSPARAVSIMATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSAVRITPQIAFSSIEKTLKPKIEFLQNLGFVGSDLSKFISRQSKFFSSSLEKTLMPNVEILKNVLPKGTCNDDLPKVLRRCSDVLTRSPYKVLSVNINYLRSCGIVDYQLSTLLKRQPALFIMHESRLKDFVSMAVKAGFSPNGTMFIHGLHSISSISNATYKKKVKLICSFGITEKECMRMFNSAPVLMRTSVGKLEVGLEFFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKSKRLTKKLPKLIDSLWMPDEDFLDKFVRRFPDNMNDLFEAFRGNSRR*
Homology
BLAST of CSPI04G04850 vs. ExPASy Swiss-Prot
Match: F4JVI3 (Transcription termination factor MTERF5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF5 PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 5.4e-10
Identity = 65/284 (22.89%), Postives = 125/284 (44.01%), Query Frame = 0

Query: 60  DTFQLSPARAVSIMATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSAVRITPQIAFSSIEK 119
           DT +L     VS + T   +    +P+++  YL DLGL+  QIK+  R      + S++ 
Sbjct: 177 DTRKLRAVSRVSELDTEGAL----RPQTL--YLLDLGLNLEQIKTITRKFAAFPYYSLDG 236

Query: 120 TLKPKIEFLQNLGFVGSDLSKFISRQSKFFSSSLEKTLMPNVEILKNVLPKGTCNDDLPK 179
            +KP +EFL +LG   SD+   + ++ +    SL   L P +  L+ +   G   +   K
Sbjct: 237 KIKPVVEFLLDLGIPKSDIPTILCKRPQICGISLTDNLKPTMAFLETL---GIDKNQWAK 296

Query: 180 VLRRCSDVLTRSPYKVLSVNINYLRSCGIVDYQLSTLLKRQPALFIMH-ESRLKDFVSMA 239
           ++ R   +LT S  K+ S  + +L   G+ + Q+  +L R P +     E +L+   +M 
Sbjct: 297 IISRFPAILTYSRQKLTS-TVEFLSQTGLTEEQIGRILTRCPNIMSYSVEDKLRP--TME 356

Query: 240 VKAGFSPNGTMFIHGLHSISSIS-NATYKKKVKLICSFGITEKECMRMFNSAPVLMRTSV 299
                + +  + +H       +S  +  K   +     G    E   M +    L   S+
Sbjct: 357 YFRSLNVDVAVLLHRCPQTFGLSIESNLKPVTEFFLEKGFGLDEIGIMISRYGALYTFSL 416

Query: 300 GKLEVGLEFFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYRVLE 342
            +  +    +       KS++V+ P    +++  R+ PRY +++
Sbjct: 417 KENVMPKWDYFQTMDYPKSELVKFPQFFGYSLQERIKPRYELVQ 448

BLAST of CSPI04G04850 vs. ExPASy Swiss-Prot
Match: Q9SZL6 (Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MTERF6 PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.7e-08
Identity = 39/144 (27.08%), Postives = 68/144 (47.22%), Query Frame = 0

Query: 83  EKPRSVYKYLSDLGLSDTQIKSAVRITPQIAFSSIEKTLKPKIEFLQNLGF-VGSDLSKF 142
           EK   +  +   LG+ +TQ+   +   P++   SI+  L   + FL +LG      + K 
Sbjct: 112 EKLCPLLAFFQALGVPETQLGKMILFNPRLISYSIDTKLTVIVSFLASLGLDQDGMIGKV 171

Query: 143 ISRQSKFFSSSLEKTLMPNVEILKNVLPKGTCNDDLPKVLRRCSDVLTRSPYKVLSVNIN 202
           + +       S++K L P  E LK+ +  G   D +  V+     +L R   K+L  N +
Sbjct: 172 LVKNPFLMGYSVDKRLRPTTEFLKSSV--GLSEDGIKSVVMNFPQLLCRDVNKILKPNYD 231

Query: 203 YLRSCGIVDYQLSTLLKRQPALFI 226
           YL+ CG  D Q++T++   P + I
Sbjct: 232 YLKECGFGDSQIATMVTGYPQILI 253

BLAST of CSPI04G04850 vs. ExPASy Swiss-Prot
Match: F4IHL3 (Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF2 PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 1.2e-06
Identity = 41/155 (26.45%), Postives = 68/155 (43.87%), Query Frame = 0

Query: 78  GIQSTEKPRSVYKYLSDLGLSDTQIKSAVRITPQIAFSSIEKTLKPKIEFLQNLGFVGSD 137
           G    E+ + + KY   LG+    +K  + + P +    +EKT+ PK+ FLQ +G     
Sbjct: 381 GCSIEERWKPLVKYFYYLGIPKEGMKRILVVKPILYCIDLEKTIAPKVRFLQEMGIPNEA 440

Query: 138 LSKFISRQSKFFSSSLEKTLMPNVEILKNVLPKGTCNDDLPKVLRRCSDVLTRSPYKVLS 197
           +   + +     ++SL K + P V  L  +   G    D+ KV+     +L  S    L 
Sbjct: 441 IGNMLVKFPSLLTNSLYKKIRPVVIFL--LTRAGVTQKDIGKVIAMDPALLGCSIGTKLE 500

Query: 198 VNINYLRSCGIVDYQLSTLLKRQPALFIMHESRLK 233
            N+ Y  S GI  YQL  ++   P L   +   L+
Sbjct: 501 PNMRYYISLGIRFYQLGEMIADFPMLLRYNVDNLR 533

BLAST of CSPI04G04850 vs. ExPASy Swiss-Prot
Match: Q9ZT96 (Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF4 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 8.0e-06
Identity = 38/142 (26.76%), Postives = 71/142 (50.00%), Query Frame = 0

Query: 90  KYLSDLGLSDTQIKSAVRITPQIAFSSIEKTLKPKIEFLQNLGFVGSDLSKFISRQSKFF 149
           ++L  LGL+   I +     P +   S++K + P +++L  LG   S  ++F+ R  +  
Sbjct: 152 EFLHKLGLTIEDINN----YPLVLGCSVKKNMVPVLDYLGKLGVRKSTFTEFLRRYPQVL 211

Query: 150 SSSLEKTLMPNVEILKNVLPKGTCNDDLPKVLRRCSDVLTRSPYKVLSVNINYLRSCGIV 209
            SS+   L P V+ L+ +  K +   D+P+VL R  +VL       +S ++ YL   G+ 
Sbjct: 212 HSSVVIDLAPVVKYLQGLDIKPS---DVPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVA 271

Query: 210 DYQLSTLLKRQPALFIMHESRL 232
             ++  +L R P +  M  +R+
Sbjct: 272 RREIGGILTRYPEILGMRVARI 286

BLAST of CSPI04G04850 vs. ExPASy Swiss-Prot
Match: Q9FM80 (Transcription termination factor MTERF9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF9 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 1.5e-04
Identity = 31/120 (25.83%), Postives = 58/120 (48.33%), Query Frame = 0

Query: 86  RSVYKYL-SDLGLSDTQIKSAVRITPQIAFSSIEKTLKPKIEFL-QNLGFVGSDLSKFIS 145
           R   +YL  ++G+ +T +   V+++PQI    ++ T   +  FL + LG     + K + 
Sbjct: 292 RPTIRYLIEEVGIKETDVGKVVQLSPQILVQRLDITWNTRYMFLSKELGAPRDSVVKMVK 351

Query: 146 RQSKFFSSSLEKTLMPNVEILKNVLPKGTCNDDLPKVLRRCSDVLTRSPYKVLSVNINYL 204
           +  +    S++   +P +  L+++   G CN D+ KVL   + VL+ S    L     YL
Sbjct: 352 KHPQLLHYSIDDGFLPRINFLRSI---GMCNSDILKVLTSLTQVLSLSLEDNLKPKYMYL 408

BLAST of CSPI04G04850 vs. ExPASy TrEMBL
Match: A0A0A0KUB9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G036600 PE=3 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 6.4e-216
Identity = 391/406 (96.31%), Postives = 391/406 (96.31%), Query Frame = 0

Query: 1   MYAMYPSFSVCVPSPLNPPFFLRLIHPFLFSFF---------------SSSSSSSSSHSQ 60
           MYAMYPSFSVCVPSPLNPPFFLRLIHPFLFSFF               SSSSSSSSSHSQ
Sbjct: 1   MYAMYPSFSVCVPSPLNPPFFLRLIHPFLFSFFSSSSSSSSSSSSSSSSSSSSSSSSHSQ 60

Query: 61  PPSHSYGIVLQYLIDTFQLSPARAVSIMATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSA 120
           PPSHSYGIVLQYLIDTFQLSPARAVSIMATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSA
Sbjct: 61  PPSHSYGIVLQYLIDTFQLSPARAVSIMATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSA 120

Query: 121 VRITPQIAFSSIEKTLKPKIEFLQNLGFVGSDLSKFISRQSKFFSSSLEKTLMPNVEILK 180
           VRITPQIAFSSIEKTLKPKIEFLQNLGFVGSDLSKFISRQSKFFSSSLEKTLMPNVEILK
Sbjct: 121 VRITPQIAFSSIEKTLKPKIEFLQNLGFVGSDLSKFISRQSKFFSSSLEKTLMPNVEILK 180

Query: 181 NVLPKGTCNDDLPKVLRRCSDVLTRSPYKVLSVNINYLRSCGIVDYQLSTLLKRQPALFI 240
           NVLPKGTCNDDLPKVLRRCSDVLTRSPYKVLSVNINYLRSCGIVDYQLSTLLKRQPALFI
Sbjct: 181 NVLPKGTCNDDLPKVLRRCSDVLTRSPYKVLSVNINYLRSCGIVDYQLSTLLKRQPALFI 240

Query: 241 MHESRLKDFVSMAVKAGFSPNGTMFIHGLHSISSISNATYKKKVKLICSFGITEKECMRM 300
           MHESRLKDFVSMAVKAGFSPNGTMFIHGLHSISSISNATYKKKVKLICSFGITEKECMRM
Sbjct: 241 MHESRLKDFVSMAVKAGFSPNGTMFIHGLHSISSISNATYKKKVKLICSFGITEKECMRM 300

Query: 301 FNSAPVLMRTSVGKLEVGLEFFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKS 360
           FNSAPVLMRTSVGKLEVGLEFFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKS
Sbjct: 301 FNSAPVLMRTSVGKLEVGLEFFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKS 360

Query: 361 KRLTKKLPKLIDSLWMPDEDFLDKFVRRFPDNMNDLFEAFRGNSRR 392
           KRLTKKLPKLIDSLWMPDEDFLDKFVRRFPDNMNDLFEAFRGNSRR
Sbjct: 361 KRLTKKLPKLIDSLWMPDEDFLDKFVRRFPDNMNDLFEAFRGNSRR 406

BLAST of CSPI04G04850 vs. ExPASy TrEMBL
Match: A0A5A7TPC3 (Transcription termination factor MTERF5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00220 PE=3 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 1.4e-170
Identity = 322/389 (82.78%), Postives = 342/389 (87.92%), Query Frame = 0

Query: 1   MYAMYPSFSVCVPSPLNPPFFLRLIHPFLFSFFSSSSSSSSSHSQPPSHSYGIVLQYLID 60
           MYAM P FS  V S LNPP FLR IHPF  SFFSSSSSSSSSH+QPPS S GIVLQYLID
Sbjct: 1   MYAMTPPFS--VSSRLNPPPFLR-IHPFFSSFFSSSSSSSSSHTQPPSDSNGIVLQYLID 60

Query: 61  TFQLSPARAVSIMATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSAVRITPQIAFSSIEKT 120
           TFQLSPA AVSIMA RRG++STEKPRSVYKYLS+LGLSDTQIKSA+R TPQ+AF+SIEKT
Sbjct: 61  TFQLSPASAVSIMANRRGLKSTEKPRSVYKYLSELGLSDTQIKSAIRNTPQLAFTSIEKT 120

Query: 121 LKPKIEFLQNLGFVGSDLSKFISRQSKFFSSSLEKTLMPNVEILKNVLPKGTCNDDLPKV 180
           LKPKIEF QNLG VGS LSKFIS+ SK  S SLEKTLMPN+EILKNVL K  CN  LP V
Sbjct: 121 LKPKIEFFQNLGLVGSHLSKFISKNSKVLSCSLEKTLMPNIEILKNVLAKDKCNAGLPTV 180

Query: 181 LRRCSDVLTRSPYKVLSVNINYLRSCGIVDYQLSTLLKRQPALFIMHESRLKDFVSMAVK 240
           LRRCS +L RSP KVLSVNI+YLRSCG+VDYQL  LLKRQPAL I  ESRLKDFVSMA+K
Sbjct: 181 LRRCSGILMRSPDKVLSVNISYLRSCGVVDYQLPMLLKRQPALLITRESRLKDFVSMAIK 240

Query: 241 AGFSPNGTMFIHGLHSISSISNATYKKKVKLICSFGITEKECMRMFNSAPVLMRTSVGKL 300
           AGFS N  MFIHGL SISSISNAT+KKKV+LICSFGITEKECMRMFNSAPVLMRTS+ KL
Sbjct: 241 AGFSINSRMFIHGLLSISSISNATFKKKVELICSFGITEKECMRMFNSAPVLMRTSIDKL 300

Query: 301 EVGLEFFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKSKRLTKKLPKLIDSLW 360
           EVGLEFFMNEAKVSKS+IVR P CLMHAMH RVLPRYRVLEVLKSKRLTKKLPKLI+SLW
Sbjct: 301 EVGLEFFMNEAKVSKSEIVRKPSCLMHAMHERVLPRYRVLEVLKSKRLTKKLPKLINSLW 360

Query: 361 MPDEDFLDKFVRRFPDNMNDLFEAFRGNS 390
             DEDFLD +VRRFPDNM+DLFEAFRGNS
Sbjct: 361 KSDEDFLDIYVRRFPDNMDDLFEAFRGNS 386

BLAST of CSPI04G04850 vs. ExPASy TrEMBL
Match: A0A1S3BXT8 (transcription termination factor MTERF5, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494394 PE=3 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 4.5e-169
Identity = 322/396 (81.31%), Postives = 343/396 (86.62%), Query Frame = 0

Query: 1   MYAMYPSFSVCVPSPLNPPFFLRLIHPFLFSFF-------SSSSSSSSSHSQPPSHSYGI 60
           MYAM P FS  V S LNPP FLR IHPF FSFF       SSSSSSSSSH+QPPS S GI
Sbjct: 1   MYAMTPPFS--VSSRLNPPPFLR-IHPFFFSFFSSSSSSSSSSSSSSSSHTQPPSDSNGI 60

Query: 61  VLQYLIDTFQLSPARAVSIMATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSAVRITPQIA 120
           VLQYLIDTFQLSPA AVSIMA RRG++STEKPRSVYKYLS+LGLSDTQIKSA+R TPQ+A
Sbjct: 61  VLQYLIDTFQLSPASAVSIMANRRGLKSTEKPRSVYKYLSELGLSDTQIKSAIRNTPQLA 120

Query: 121 FSSIEKTLKPKIEFLQNLGFVGSDLSKFISRQSKFFSSSLEKTLMPNVEILKNVLPKGTC 180
           F+SI+KTLKPKIEF QNLG VGS LSKFIS+ SK  S SLEKTLMPN+EILKNVL K  C
Sbjct: 121 FTSIDKTLKPKIEFFQNLGLVGSHLSKFISKNSKVLSCSLEKTLMPNIEILKNVLAKDKC 180

Query: 181 NDDLPKVLRRCSDVLTRSPYKVLSVNINYLRSCGIVDYQLSTLLKRQPALFIMHESRLKD 240
           N  LP VLRRCS +L RSP KVLSVNI+YLRSCG+VDYQL  LLKRQPAL I  ESRLKD
Sbjct: 181 NAGLPTVLRRCSGILMRSPDKVLSVNISYLRSCGVVDYQLPMLLKRQPALLITRESRLKD 240

Query: 241 FVSMAVKAGFSPNGTMFIHGLHSISSISNATYKKKVKLICSFGITEKECMRMFNSAPVLM 300
           FVSMA+KAGFS N  MFIHGL SISSISNAT+KKKV+LICSFGITEKECMRMFNSAPVLM
Sbjct: 241 FVSMAIKAGFSINSRMFIHGLLSISSISNATFKKKVELICSFGITEKECMRMFNSAPVLM 300

Query: 301 RTSVGKLEVGLEFFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKSKRLTKKLP 360
           RTS+ KLEVGLEFFMNEAKVSKS+IVR P CLMHAMH RVLPRYRVLEVLKSKRLTKKLP
Sbjct: 301 RTSIDKLEVGLEFFMNEAKVSKSEIVRKPSCLMHAMHERVLPRYRVLEVLKSKRLTKKLP 360

Query: 361 KLIDSLWMPDEDFLDKFVRRFPDNMNDLFEAFRGNS 390
           KLI+SLW  DEDFLD +VRRFPDNM+DLFEAFRGNS
Sbjct: 361 KLINSLWKSDEDFLDIYVRRFPDNMDDLFEAFRGNS 393

BLAST of CSPI04G04850 vs. ExPASy TrEMBL
Match: A0A5D3CYZ2 (Transcription termination factor MTERF5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G005620 PE=3 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 4.5e-169
Identity = 322/396 (81.31%), Postives = 343/396 (86.62%), Query Frame = 0

Query: 1   MYAMYPSFSVCVPSPLNPPFFLRLIHPFLFSFF-------SSSSSSSSSHSQPPSHSYGI 60
           MYAM P FS  V S LNPP FLR IHPF FSFF       SSSSSSSSSH+QPPS S GI
Sbjct: 1   MYAMTPPFS--VSSRLNPPPFLR-IHPFFFSFFSSSSSSSSSSSSSSSSHTQPPSDSNGI 60

Query: 61  VLQYLIDTFQLSPARAVSIMATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSAVRITPQIA 120
           VLQYLIDTFQLSPA AVSIMA RRG++STEKPRSVYKYLS+LGLSDTQIKSA+R TPQ+A
Sbjct: 61  VLQYLIDTFQLSPASAVSIMANRRGLKSTEKPRSVYKYLSELGLSDTQIKSAIRNTPQLA 120

Query: 121 FSSIEKTLKPKIEFLQNLGFVGSDLSKFISRQSKFFSSSLEKTLMPNVEILKNVLPKGTC 180
           F+SI+KTLKPKIEF QNLG VGS LSKFIS+ SK  S SLEKTLMPN+EILKNVL K  C
Sbjct: 121 FTSIDKTLKPKIEFFQNLGLVGSHLSKFISKNSKVLSCSLEKTLMPNIEILKNVLAKDKC 180

Query: 181 NDDLPKVLRRCSDVLTRSPYKVLSVNINYLRSCGIVDYQLSTLLKRQPALFIMHESRLKD 240
           N  LP VLRRCS +L RSP KVLSVNI+YLRSCG+VDYQL  LLKRQPAL I  ESRLKD
Sbjct: 181 NAGLPTVLRRCSGILMRSPDKVLSVNISYLRSCGVVDYQLPMLLKRQPALLITRESRLKD 240

Query: 241 FVSMAVKAGFSPNGTMFIHGLHSISSISNATYKKKVKLICSFGITEKECMRMFNSAPVLM 300
           FVSMA+KAGFS N  MFIHGL SISSISNAT+KKKV+LICSFGITEKECMRMFNSAPVLM
Sbjct: 241 FVSMAIKAGFSINSRMFIHGLLSISSISNATFKKKVELICSFGITEKECMRMFNSAPVLM 300

Query: 301 RTSVGKLEVGLEFFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKSKRLTKKLP 360
           RTS+ KLEVGLEFFMNEAKVSKS+IVR P CLMHAMH RVLPRYRVLEVLKSKRLTKKLP
Sbjct: 301 RTSIDKLEVGLEFFMNEAKVSKSEIVRKPSCLMHAMHERVLPRYRVLEVLKSKRLTKKLP 360

Query: 361 KLIDSLWMPDEDFLDKFVRRFPDNMNDLFEAFRGNS 390
           KLI+SLW  DEDFLD +VRRFPDNM+DLFEAFRGNS
Sbjct: 361 KLINSLWKSDEDFLDIYVRRFPDNMDDLFEAFRGNS 393

BLAST of CSPI04G04850 vs. ExPASy TrEMBL
Match: A0A6J1JB80 (uncharacterized protein LOC111482884 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482884 PE=3 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 2.3e-141
Identity = 273/387 (70.54%), Postives = 316/387 (81.65%), Query Frame = 0

Query: 1   MYAMYPSFSVCVPSPLNPPFFLRLIHPFLFSFFSSSSSSSSSHSQPPSHSYGIVLQYLID 60
           MYA+  SFS  + S  NPP  LR I P L+ FF     SSSSHSQ  S SYGIV+QYLID
Sbjct: 1   MYALRSSFS--ISSRHNPPSLLR-IDPLLYCFF-----SSSSHSQ-ASASYGIVVQYLID 60

Query: 61  TFQLSPARAVSIMATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSAVRITPQIAFSSIEKT 120
           TF+LSPARAVSIM++RRGI STEKP+SVYKYLS+LG S+  I+S +R+ PQIAFS+IEKT
Sbjct: 61  TFELSPARAVSIMSSRRGIASTEKPQSVYKYLSELGFSEAHIQSTIRLAPQIAFSNIEKT 120

Query: 121 LKPKIEFLQNLGFVGSDLSKFISRQSKFFSSSLEKTLMPNVEILKNVLPKGTCNDDLPKV 180
           LKPKIEF QNLG VGSDL +FIS+ S   + SLEK LMP+VEILK+V PK  CN DL +V
Sbjct: 121 LKPKIEFFQNLGLVGSDLGRFISKHSTLLTISLEKRLMPSVEILKSVFPKDECNSDLLQV 180

Query: 181 LRRCSDVLTRSPYKVLSVNINYLRSCGIVDYQLSTLLKRQPALFIMHESRLKDFVSMAVK 240
           LRRCSD+L RSPY  L VNINYL+SCGIV  QLS LLKRQPALF M ES+++D VSM V+
Sbjct: 181 LRRCSDMLMRSPYTRLPVNINYLQSCGIVGSQLSMLLKRQPALFGMRESQVRDLVSMVVE 240

Query: 241 AGFSPNGTMFIHGLHSISSISNATYKKKVKLICSFGITEKECMRMFNSAPVLMRTSVGKL 300
            GFS N  MF+HGLH+ISS+SNAT+KKKV+LICSFGITEKECMRMF SAPVL+RTSVGKL
Sbjct: 241 TGFSTNTRMFVHGLHAISSVSNATFKKKVELICSFGITEKECMRMFTSAPVLIRTSVGKL 300

Query: 301 EVGLEFFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKSKRLTKKLPKLIDSLW 360
           + GLEFFMNEA+VS+SDIVR P CLMHAM GRVLPRYRVL+V+ SKRL+KK  +LID L 
Sbjct: 301 KRGLEFFMNEAEVSRSDIVRKPTCLMHAMQGRVLPRYRVLQVVNSKRLSKKHLQLIDILG 360

Query: 361 MPDEDFLDKFVRRFPDNMNDLFEAFRG 388
           M DE+FLDKFV RFPD++ +L  AFRG
Sbjct: 361 MSDENFLDKFVHRFPDHVTELLAAFRG 378

BLAST of CSPI04G04850 vs. NCBI nr
Match: XP_004149163.2 (transcription termination factor MTERF5, chloroplastic [Cucumis sativus] >KAE8637573.1 hypothetical protein CSA_017850 [Cucumis sativus])

HSP 1 Score: 764.6 bits (1973), Expect = 4.1e-217
Identity = 391/393 (99.49%), Postives = 391/393 (99.49%), Query Frame = 0

Query: 1   MYAMYPSFSVCVPSPLNPPFFLRLIHPFLFSFF--SSSSSSSSSHSQPPSHSYGIVLQYL 60
           MYAMYPSFSVCVPSPLNPPFFLRLIHPFLFSFF  SSSSSSSSSHSQPPSHSYGIVLQYL
Sbjct: 1   MYAMYPSFSVCVPSPLNPPFFLRLIHPFLFSFFSSSSSSSSSSSHSQPPSHSYGIVLQYL 60

Query: 61  IDTFQLSPARAVSIMATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSAVRITPQIAFSSIE 120
           IDTFQLSPARAVSIMATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSAVRITPQIAFSSIE
Sbjct: 61  IDTFQLSPARAVSIMATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSAVRITPQIAFSSIE 120

Query: 121 KTLKPKIEFLQNLGFVGSDLSKFISRQSKFFSSSLEKTLMPNVEILKNVLPKGTCNDDLP 180
           KTLKPKIEFLQNLGFVGSDLSKFISRQSKFFSSSLEKTLMPNVEILKNVLPKGTCNDDLP
Sbjct: 121 KTLKPKIEFLQNLGFVGSDLSKFISRQSKFFSSSLEKTLMPNVEILKNVLPKGTCNDDLP 180

Query: 181 KVLRRCSDVLTRSPYKVLSVNINYLRSCGIVDYQLSTLLKRQPALFIMHESRLKDFVSMA 240
           KVLRRCSDVLTRSPYKVLSVNINYLRSCGIVDYQLSTLLKRQPALFIMHESRLKDFVSMA
Sbjct: 181 KVLRRCSDVLTRSPYKVLSVNINYLRSCGIVDYQLSTLLKRQPALFIMHESRLKDFVSMA 240

Query: 241 VKAGFSPNGTMFIHGLHSISSISNATYKKKVKLICSFGITEKECMRMFNSAPVLMRTSVG 300
           VKAGFSPNGTMFIHGLHSISSISNATYKKKVKLICSFGITEKECMRMFNSAPVLMRTSVG
Sbjct: 241 VKAGFSPNGTMFIHGLHSISSISNATYKKKVKLICSFGITEKECMRMFNSAPVLMRTSVG 300

Query: 301 KLEVGLEFFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKSKRLTKKLPKLIDS 360
           KLEVGLEFFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKSKRLTKKLPKLIDS
Sbjct: 301 KLEVGLEFFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKSKRLTKKLPKLIDS 360

Query: 361 LWMPDEDFLDKFVRRFPDNMNDLFEAFRGNSRR 392
           LWMPDEDFLDKFVRRFPDNMNDLFEAFRGNSRR
Sbjct: 361 LWMPDEDFLDKFVRRFPDNMNDLFEAFRGNSRR 393

BLAST of CSPI04G04850 vs. NCBI nr
Match: KAA0044738.1 (transcription termination factor MTERF5 [Cucumis melo var. makuwa])

HSP 1 Score: 609.0 bits (1569), Expect = 2.9e-170
Identity = 322/389 (82.78%), Postives = 342/389 (87.92%), Query Frame = 0

Query: 1   MYAMYPSFSVCVPSPLNPPFFLRLIHPFLFSFFSSSSSSSSSHSQPPSHSYGIVLQYLID 60
           MYAM P FS  V S LNPP FLR IHPF  SFFSSSSSSSSSH+QPPS S GIVLQYLID
Sbjct: 1   MYAMTPPFS--VSSRLNPPPFLR-IHPFFSSFFSSSSSSSSSHTQPPSDSNGIVLQYLID 60

Query: 61  TFQLSPARAVSIMATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSAVRITPQIAFSSIEKT 120
           TFQLSPA AVSIMA RRG++STEKPRSVYKYLS+LGLSDTQIKSA+R TPQ+AF+SIEKT
Sbjct: 61  TFQLSPASAVSIMANRRGLKSTEKPRSVYKYLSELGLSDTQIKSAIRNTPQLAFTSIEKT 120

Query: 121 LKPKIEFLQNLGFVGSDLSKFISRQSKFFSSSLEKTLMPNVEILKNVLPKGTCNDDLPKV 180
           LKPKIEF QNLG VGS LSKFIS+ SK  S SLEKTLMPN+EILKNVL K  CN  LP V
Sbjct: 121 LKPKIEFFQNLGLVGSHLSKFISKNSKVLSCSLEKTLMPNIEILKNVLAKDKCNAGLPTV 180

Query: 181 LRRCSDVLTRSPYKVLSVNINYLRSCGIVDYQLSTLLKRQPALFIMHESRLKDFVSMAVK 240
           LRRCS +L RSP KVLSVNI+YLRSCG+VDYQL  LLKRQPAL I  ESRLKDFVSMA+K
Sbjct: 181 LRRCSGILMRSPDKVLSVNISYLRSCGVVDYQLPMLLKRQPALLITRESRLKDFVSMAIK 240

Query: 241 AGFSPNGTMFIHGLHSISSISNATYKKKVKLICSFGITEKECMRMFNSAPVLMRTSVGKL 300
           AGFS N  MFIHGL SISSISNAT+KKKV+LICSFGITEKECMRMFNSAPVLMRTS+ KL
Sbjct: 241 AGFSINSRMFIHGLLSISSISNATFKKKVELICSFGITEKECMRMFNSAPVLMRTSIDKL 300

Query: 301 EVGLEFFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKSKRLTKKLPKLIDSLW 360
           EVGLEFFMNEAKVSKS+IVR P CLMHAMH RVLPRYRVLEVLKSKRLTKKLPKLI+SLW
Sbjct: 301 EVGLEFFMNEAKVSKSEIVRKPSCLMHAMHERVLPRYRVLEVLKSKRLTKKLPKLINSLW 360

Query: 361 MPDEDFLDKFVRRFPDNMNDLFEAFRGNS 390
             DEDFLD +VRRFPDNM+DLFEAFRGNS
Sbjct: 361 KSDEDFLDIYVRRFPDNMDDLFEAFRGNS 386

BLAST of CSPI04G04850 vs. NCBI nr
Match: XP_008453751.1 (PREDICTED: transcription termination factor MTERF5, chloroplastic [Cucumis melo] >XP_008453752.1 PREDICTED: transcription termination factor MTERF5, chloroplastic [Cucumis melo] >XP_008453753.1 PREDICTED: transcription termination factor MTERF5, chloroplastic [Cucumis melo] >XP_008453754.1 PREDICTED: transcription termination factor MTERF5, chloroplastic [Cucumis melo] >XP_008453755.1 PREDICTED: transcription termination factor MTERF5, chloroplastic [Cucumis melo] >XP_008453756.1 PREDICTED: transcription termination factor MTERF5, chloroplastic [Cucumis melo] >XP_008453758.1 PREDICTED: transcription termination factor MTERF5, chloroplastic [Cucumis melo] >TYK16725.1 transcription termination factor MTERF5 [Cucumis melo var. makuwa])

HSP 1 Score: 604.0 bits (1556), Expect = 9.3e-169
Identity = 322/396 (81.31%), Postives = 343/396 (86.62%), Query Frame = 0

Query: 1   MYAMYPSFSVCVPSPLNPPFFLRLIHPFLFSFF-------SSSSSSSSSHSQPPSHSYGI 60
           MYAM P FS  V S LNPP FLR IHPF FSFF       SSSSSSSSSH+QPPS S GI
Sbjct: 1   MYAMTPPFS--VSSRLNPPPFLR-IHPFFFSFFSSSSSSSSSSSSSSSSHTQPPSDSNGI 60

Query: 61  VLQYLIDTFQLSPARAVSIMATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSAVRITPQIA 120
           VLQYLIDTFQLSPA AVSIMA RRG++STEKPRSVYKYLS+LGLSDTQIKSA+R TPQ+A
Sbjct: 61  VLQYLIDTFQLSPASAVSIMANRRGLKSTEKPRSVYKYLSELGLSDTQIKSAIRNTPQLA 120

Query: 121 FSSIEKTLKPKIEFLQNLGFVGSDLSKFISRQSKFFSSSLEKTLMPNVEILKNVLPKGTC 180
           F+SI+KTLKPKIEF QNLG VGS LSKFIS+ SK  S SLEKTLMPN+EILKNVL K  C
Sbjct: 121 FTSIDKTLKPKIEFFQNLGLVGSHLSKFISKNSKVLSCSLEKTLMPNIEILKNVLAKDKC 180

Query: 181 NDDLPKVLRRCSDVLTRSPYKVLSVNINYLRSCGIVDYQLSTLLKRQPALFIMHESRLKD 240
           N  LP VLRRCS +L RSP KVLSVNI+YLRSCG+VDYQL  LLKRQPAL I  ESRLKD
Sbjct: 181 NAGLPTVLRRCSGILMRSPDKVLSVNISYLRSCGVVDYQLPMLLKRQPALLITRESRLKD 240

Query: 241 FVSMAVKAGFSPNGTMFIHGLHSISSISNATYKKKVKLICSFGITEKECMRMFNSAPVLM 300
           FVSMA+KAGFS N  MFIHGL SISSISNAT+KKKV+LICSFGITEKECMRMFNSAPVLM
Sbjct: 241 FVSMAIKAGFSINSRMFIHGLLSISSISNATFKKKVELICSFGITEKECMRMFNSAPVLM 300

Query: 301 RTSVGKLEVGLEFFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKSKRLTKKLP 360
           RTS+ KLEVGLEFFMNEAKVSKS+IVR P CLMHAMH RVLPRYRVLEVLKSKRLTKKLP
Sbjct: 301 RTSIDKLEVGLEFFMNEAKVSKSEIVRKPSCLMHAMHERVLPRYRVLEVLKSKRLTKKLP 360

Query: 361 KLIDSLWMPDEDFLDKFVRRFPDNMNDLFEAFRGNS 390
           KLI+SLW  DEDFLD +VRRFPDNM+DLFEAFRGNS
Sbjct: 361 KLINSLWKSDEDFLDIYVRRFPDNMDDLFEAFRGNS 393

BLAST of CSPI04G04850 vs. NCBI nr
Match: XP_031739970.1 (transcription termination factor MTERF5, chloroplastic-like [Cucumis sativus] >KAE8649160.1 hypothetical protein Csa_014489 [Cucumis sativus])

HSP 1 Score: 580.9 bits (1496), Expect = 8.4e-162
Identity = 298/319 (93.42%), Postives = 300/319 (94.04%), Query Frame = 0

Query: 73  MATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSAVRITPQIAFSSIEKTLKPKIEFLQNLG 132
           MATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSAVRITPQIAFSSIEKTLKPKIEFLQNLG
Sbjct: 1   MATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSAVRITPQIAFSSIEKTLKPKIEFLQNLG 60

Query: 133 FVGSDLSKFISRQSKFFSSSLEKTLMPNVEILKNVLPKGTCNDDLPKVLRRCSDVLTRSP 192
           FVGSDL+                 +  NVEILKNVLPKGTCNDDLPKVLRRCSDVLTRSP
Sbjct: 61  FVGSDLN-----------------IDANVEILKNVLPKGTCNDDLPKVLRRCSDVLTRSP 120

Query: 193 YKVLSVNINYLRSCGIVDYQLSTLLKRQPALFIMHESRLKDFVSMAVKAGFSPNGTMFIH 252
           YKVLSVNINYLRSCGIVDYQLSTLLKRQPALFIMHESRLKDFVSMAVKAGFSPNGTMFIH
Sbjct: 121 YKVLSVNINYLRSCGIVDYQLSTLLKRQPALFIMHESRLKDFVSMAVKAGFSPNGTMFIH 180

Query: 253 GLHSISSISNATYKKKVKLICSFGITEKECMRMFNSAPVLMRTSVGKLEVGLEFFMNEAK 312
           GLHSISSISNATYKKKVKLICSFGITEKECMRMFNSAPVLMRTSVGKLEVGLEFFMNEAK
Sbjct: 181 GLHSISSISNATYKKKVKLICSFGITEKECMRMFNSAPVLMRTSVGKLEVGLEFFMNEAK 240

Query: 313 VSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKSKRLTKKLPKLIDSLWMPDEDFLDKFVR 372
           VSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKSKRLTKKLPKLIDSLWMPDEDFLDKFVR
Sbjct: 241 VSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKSKRLTKKLPKLIDSLWMPDEDFLDKFVR 300

Query: 373 RFPDNMNDLFEAFRGNSRR 392
           RFPDNMNDLFEAFRGNSRR
Sbjct: 301 RFPDNMNDLFEAFRGNSRR 302

BLAST of CSPI04G04850 vs. NCBI nr
Match: XP_038888519.1 (transcription termination factor MTERF5, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 531.6 bits (1368), Expect = 5.9e-147
Identity = 284/389 (73.01%), Postives = 320/389 (82.26%), Query Frame = 0

Query: 1   MYAMYPSFSVCVPSPLNPPFFLRLIHPFLFSFFSSSSSSSSSHSQPPSHSYGIVLQYLID 60
           MYA+ PSFS  V S LNPP FLR IHPFL+ FFSSSSSSS + S   S S  IV+QYLID
Sbjct: 1   MYALRPSFS--VSSRLNPPSFLR-IHPFLYYFFSSSSSSSHTQS---SASNAIVVQYLID 60

Query: 61  TFQLSPARAVSIMATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSAVRITPQIAFSSIEKT 120
           TFQLSPARA SIM+ RRG+QSTEKP+SVYKYLSDLG SD  I+SA+R+ PQIAFS+IEKT
Sbjct: 61  TFQLSPARAASIMSCRRGLQSTEKPQSVYKYLSDLGFSDAHIRSAIRLAPQIAFSNIEKT 120

Query: 121 LKPKIEFLQNLGFVGSDLSKFISRQSKFFSSSLEKTLMPNVEILKNVLPKGTCNDDLPKV 180
           LKPKIEF QNLG VGSDLSKFISR S   + SLEK LMP+VEILKN+LPK  CNDDL  V
Sbjct: 121 LKPKIEFFQNLGLVGSDLSKFISRHSTLLTVSLEKKLMPSVEILKNILPKDNCNDDLLLV 180

Query: 181 LRRCSDVLTRSPYKVLSVNINYLRSCGIVDYQLSTLLKRQPALFIMHESRLKDFVSMAVK 240
           +RR SD L R+P K LS+NINYL+SCGIVD QL  LLKRQ +LF   ESRLKD VS+ V+
Sbjct: 181 IRRWSDRLMRTPCKRLSININYLQSCGIVDSQLLMLLKRQSSLFGRSESRLKDIVSVVVE 240

Query: 241 AGFSPNGTMFIHGLHSISSISNATYKKKVKLICSFGITEKECMRMFNSAPVLMRTSVGKL 300
            GFS N  MF+HGLH+ISS+S  T KKKV+LICSFGITEKECMRMF SAPVL+RTS+GKL
Sbjct: 241 IGFSTNTRMFVHGLHAISSVSKVTLKKKVELICSFGITEKECMRMFTSAPVLIRTSIGKL 300

Query: 301 EVGLEFFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKSKRLTKKLPKLIDSLW 360
           ++GLEFFMNEAKVSKSDIV +P CLMHAM GRVLPRYRVL+++KSKRL KK PK I +L 
Sbjct: 301 KLGLEFFMNEAKVSKSDIVGSPACLMHAMQGRVLPRYRVLQIVKSKRLMKKHPKFILTLG 360

Query: 361 MPDEDFLDKFVRRFPDNMNDLFEAFRGNS 390
           M DEDFLD+FV RFPDNM DL  A+RGNS
Sbjct: 361 MSDEDFLDRFVCRFPDNMKDLLVAYRGNS 383

BLAST of CSPI04G04850 vs. TAIR 10
Match: AT5G64950.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 231.1 bits (588), Expect = 1.5e-60
Identity = 133/353 (37.68%), Postives = 215/353 (60.91%), Query Frame = 0

Query: 41  SSHSQPPSHSYGIVLQYLIDTFQLSPARAVSIMATRRGIQSTEKPRSVYKYLSDLGLSDT 100
           SS++ P S S    +++L D     P  A++I      ++S E+PRSV + L     SDT
Sbjct: 28  SSNATPASISNPAFVEFLRDNGFQKPL-AIAIAMRYPNLKSLEQPRSVIQMLKSYSFSDT 87

Query: 101 QIKSAVRITPQIAFSSIEKTLKPKIEFLQNLGFVGSDLSKFISRQSKFFSSSLEKTLMPN 160
           QI+ ++R+ P++ F ++EK L+PK+ F +++GF GS L KF+S+ S     SL K L+P 
Sbjct: 88  QIQKSIRVHPRMMFYNVEKILEPKLRFFKDIGFTGSGLGKFVSQNSSVVGVSLVKKLIPT 147

Query: 161 VEILKNVL-PKGTCNDDLPKVLRRCS-DVLTRSPYKVLSVNINYLRSCGIVDYQLSTLLK 220
           VEILK+++ PK   ++DLP +L RC   +L+R P   L  NI+YL +CGIV  QL++LL+
Sbjct: 148 VEILKSIVAPK---HEDLPVILSRCGWLLLSRDPNLFLLPNISYLETCGIVGSQLASLLR 207

Query: 221 RQPALFIMHESRLKDFVSMAVKAGFSPNGTMFIHGLHSISSISNATYKKKVKLICSFGIT 280
           RQP +F + E +L+ +VS A+  GF+ N  M +H + S+SS+S  T+ +KVKL  + G +
Sbjct: 208 RQPRIFNLSEEKLRGYVSRALDLGFTLNSRMLVHAVISLSSLSEKTFDRKVKLFMANGFS 267

Query: 281 EKECMRMFNSAPVLMRTSVGKLEVGLEFFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYR 340
           E E   +   +P L+R S  KL +G EF++    + +  + + P  L + +  RV+PR +
Sbjct: 268 EDEITDIIRRSPGLIRCSEDKLTLGFEFYLKRMGLEREALAKRPCVLSYNLEKRVIPRLK 327

Query: 341 VLEVLKSKRL----TKKLPKLIDSLWMPDEDFLDKFVRRFPDNM-NDLFEAFR 387
           VL++L+ K L     KK   ++  + M +E FL+K+V RF D +  +L  A++
Sbjct: 328 VLQILREKGLLLKEEKKKKNMVGIVEMTEEAFLEKYVVRFGDEIAEELLVAYK 376

BLAST of CSPI04G04850 vs. TAIR 10
Match: AT1G21150.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 142.5 bits (358), Expect = 7.2e-34
Identity = 100/356 (28.09%), Postives = 168/356 (47.19%), Query Frame = 0

Query: 34  SSSSSSSSSHSQPPSHSYGIVLQYLIDTFQLSPARAVSIMATRRGIQSTEKPRSVYKYLS 93
           S   S +    +P   S+   + YL+D+  LS   A S     + + S++KP SV     
Sbjct: 40  SQVKSENLEKQKPCLESF--TVSYLVDSCGLSLESAKSNSRFVK-LVSSKKPDSVLALFK 99

Query: 94  DLGLSDTQIKSAVRITPQIAFSSIEKTLKPKIEFLQNLGFVGSDLSKFISRQSKFFSSSL 153
           D G ++ QI S ++  P++   S E  + PK+ F  ++GF  SD +K IS   K  S SL
Sbjct: 100 DHGFTNDQITSVIKSFPRVLSLSPEDVISPKLMFFSSIGFSTSDTAKMISSSPKMLSYSL 159

Query: 154 EKTLMPNVEILKNVLPKGTCNDDLPKVLRRCSDVLTRSPYKVLSVNINYLRSCGIVDYQL 213
            K L+P  + LK++L +    + + K L+R     +      +S+ ++  R  G+ D  +
Sbjct: 160 HKRLIPCYDSLKSILVE---EESVVKCLKRGIRCFSLKITHCVSLRVSICRELGVPDKSI 219

Query: 214 STLLKRQPALFIMHESRLKDFVSMAVKAGFSPNGTMFIHGLHSISSISNATYKKKVKLIC 273
             L++  P  F   E R  + ++     GF P    F+H + +    S +  ++K KL  
Sbjct: 220 KWLVQASPFTFFSRERRFNEVLNRVCSYGFDPKKAGFVHAMVAFDCTSESAMERKFKLFQ 279

Query: 274 SFGITEKECMRMFNSAPVLMRTSVGKLEVGLEFFMNEAKVSKSDIVRNPFCLMHAMHGRV 333
            FG ++++ +      P  +  S  K+   LE+ +N   +   DIV  P  L  +M  R+
Sbjct: 280 CFGWSKEDFVAAIMRFPNCVTVSDEKIMYTLEYLVNNIGLQARDIVARPVVLSLSMEKRI 339

Query: 334 LPRYRVLEVLKSKRLTKKLP-KLIDSLWMPDEDFLDKFVRRFPDNMNDLFEAFRGN 389
            PR +V+ +L SK L KK        L +   +F+DKFV ++ D M  L + F  N
Sbjct: 340 KPRNQVISLLLSKGLVKKEDINYFTILKLKSSEFMDKFVLKYQDEMPQLVQPFTSN 389

BLAST of CSPI04G04850 vs. TAIR 10
Match: AT5G07900.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 135.6 bits (340), Expect = 8.7e-32
Identity = 100/382 (26.18%), Postives = 178/382 (46.60%), Query Frame = 0

Query: 7   SFSVCVPSPLNPPFFLRLIHPFLFSFFSSSSSSSSSHSQPPSHSYGIVLQYLIDTFQLSP 66
           SF+  +P     P F+  +    FS   ++ +S +             L YLID+  LSP
Sbjct: 25  SFTTILPKHHRKPIFVFSLQT-QFSSPVTTLTSKNPKDDEQQQKLSFTLNYLIDSCGLSP 84

Query: 67  ARAVSIMATRRGIQSTEKPRSVYKYLSDLGLSDTQIKSAVRITPQIAFSSIEKTLKPKIE 126
             A ++ + +  + S E+P +V   L D G +  QI S V+  P +  ++ E  L PK+ 
Sbjct: 85  DSA-TVASRKLLLDSPERPNTVLNLLRDHGFTTAQISSLVKKRPVLLLANAESVLLPKLS 144

Query: 127 FLQNLGFVGSDLSKFISRQSKFFSSSLEKTLMPNVEILKNVLPKGTCNDDLPKVLRRCSD 186
           F  ++G   S L++ ++      + SL   L+P+   LK+VL     ++ +   LRR + 
Sbjct: 145 FFLSIGVSKSLLARTLASDPTILTRSLVNQLIPSYNFLKSVLDS---DEKIVAALRRTTW 204

Query: 187 VLTRSPYKVLSVNINYLRSCGIVDYQLSTLLKRQPALFIMHESRLKDFVSMAVKAGFSPN 246
           V      K L  NINY+   G+ +  +  LL   P   +      +     A + GF+P 
Sbjct: 205 VFLEDHTKNLVPNINYMAETGVPEKCIKLLLTHFPEAVMQKNHEFQAIAKQAQEMGFNPQ 264

Query: 247 GTMFIHGLHSISSISN-ATYKKKVKLICSFGITEKECMRMFNSAPVLMRTSVGKLEVGLE 306
            + F+  +H++S   N + + K  ++   +G +E + M  F   P  M  S  K+   +E
Sbjct: 265 KSTFVLAIHALSGKGNKSIWDKCFEVYQRWGWSEDDIMCAFKKHPHCMMLSERKINRTME 324

Query: 307 FFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKSKRLTKKLPKLIDSLWMPDED 366
           +F+NE  ++   I + P  L  ++  R++PR  V +VL S  L K+   L   L   ++ 
Sbjct: 325 YFVNEMNMAPRSIAQCPVVLFFSLEKRIIPRCSVTKVLVSNGLVKEDWSLTSLLVPVEKV 384

Query: 367 FLDKFVRRFPDNMNDLFEAFRG 388
           FL+K V ++ + + +L   + G
Sbjct: 385 FLEKLVIKYEEELPELMNLYLG 401

BLAST of CSPI04G04850 vs. TAIR 10
Match: AT1G62120.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 98.6 bits (244), Expect = 1.2e-20
Identity = 95/394 (24.11%), Postives = 168/394 (42.64%), Query Frame = 0

Query: 33  FSSSSSSSSSHSQPPSHSYGIVLQYLIDTFQLSPARAVSIMATRRGIQSTEKPRSVYKYL 92
           FSSS+ ++   S+     +   + YL+D+  L+   A SI + +    +   P SV   L
Sbjct: 37  FSSSAIAADVSSRDGRKGHNFTVSYLVDSLGLATKVAESI-SMKVSFDNKGNPDSVLSLL 96

Query: 93  SDLGLSDTQIKSAVRITPQIAFSSIEKTLKPKIEFLQNLGFVGSDLSKFISRQSKFFSSS 152
              G +D+QI + +R  P++     EK+L PK++FLQ++G   S+L++ +S   K     
Sbjct: 97  RSHGFTDSQISNIIRTFPRLLILDAEKSLAPKLQFLQSIGASSSELTETVSAVPKILGKR 156

Query: 153 LEKTLMPNVEILKNVLPKGTCNDDLPKVLRRCSDVLTRSPYKVLSVNINYLRSCGIVDYQ 212
             K+L    + +K ++      D   K+ + C  +   S  +    N+  LR  G+    
Sbjct: 157 KGKSLSRYYDFVKVIIEA----DKSSKLEKLCHSLPEGSKQENKIRNLLVLREMGVPQRL 216

Query: 213 LSTLLKRQPALFIMHESRLKDFVSMAVKAGFSPNGTMFIHGLHSISSISNATYKKKVKLI 272
           L +LL    A  +  + + K+ +  AV+ GF P    F+  L+ +  +S+   + K    
Sbjct: 217 LFSLL-ISDAGDVCGKEKFKESLKKAVEIGFDPTTATFVKALNVLYGLSDKGIENKFNAC 276

Query: 273 CSFGITEKECMRMFNSAPVLMRTSVGKLE------VGL---------------------- 332
              G+   +   MF   P ++  S  K+E      +GL                      
Sbjct: 277 KRLGLAVDDVWAMFKKWPNILTKSEKKIENSVETFLGLGFSRDEFLMMVKRFPQCIGYST 336

Query: 333 -------EFFMNEAKVSKSDIVRNPFCLMHAMHGRVLPRYRVLEVLKSKRLTK-KLPKLI 389
                  EF + E       +   P  L +++  R +PR  V++VL SK L + +LP + 
Sbjct: 337 ELMKTKTEFLVTEMNWPLKAVASIPQVLGYSLEKRTVPRCNVIKVLISKGLLESELPPIS 396

BLAST of CSPI04G04850 vs. TAIR 10
Match: AT1G61970.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 96.3 bits (238), Expect = 5.9e-20
Identity = 95/399 (23.81%), Postives = 176/399 (44.11%), Query Frame = 0

Query: 31  SFFSSSSSSSSSHSQPPSHSYGIVLQYLIDTFQLSPARAVSIMATRRGIQSTEKPRSVYK 90
           +FFSS+S++  S  +         + YL+D+  L+   A SI + +   +    P SV  
Sbjct: 32  NFFSSASAACLS-PRVGRKGNNFTVSYLVDSLGLTTKLAESI-SRKVSFEDKNNPDSVLN 91

Query: 91  YLSDLGLSDTQIKSAVRITPQIAFSSIEKTLKPKIEFLQNLGFVGSDLSKFISRQSKFFS 150
            L+  G + +QI + +R  PQ+  +  EK+L PK++FLQ+ G   S++++ +S   +   
Sbjct: 92  LLTSHGFTGSQISTIIRDYPQLLIADAEKSLGPKLQFLQSRGASSSEITEIVSSVPEILG 151

Query: 151 SSLEKTLMPNVEILKN-VLPKGTCNDDLPKVLRRCSDVLTRSPYKVLSVNINYLRSCGIV 210
               KT+    + +K+ +L K + N+ L      C   L +   +    N++ LR  G+ 
Sbjct: 152 KKGHKTISVYYDFIKDTLLEKSSKNEKL------CHS-LPQGNLENKIRNVSVLRELGMP 211

Query: 211 DYQLSTLL--KRQPALFIMHESRLKDFVSMAVKAGFSPNGTMFIHGLHSISSISNATYKK 270
              L +LL    QP   +  + + ++ +   V+ GF P  + F+  L  I  ++  T ++
Sbjct: 212 HKLLFSLLISDSQP---VCGKEKFEETLKKVVEMGFDPTTSKFVEALQVIYKMNEKTIEE 271

Query: 271 KVKLICSFGITEKECMRMFNSAPVLMRTSVGKLEVGLEFFM------------------- 330
           KV L  S G    +    F   P+ +R S  K+   +E F+                   
Sbjct: 272 KVHLYKSLGFDVGDVWSSFKKWPISLRVSEKKMLDSIETFLGLGFSRDEFAKMVKHFPPC 331

Query: 331 -----------NEAKVSKSD-----IVRNPFCLMHAMHGRVLPRYRVLEVLKSKRLTK-K 389
                       E  V K +     +V NP    +++  R++PR  V++ L SK L + +
Sbjct: 332 IGLSTETVKKKTEFLVKKMNWPLKAVVSNPAVFGYSLEKRIVPRGNVIKALMSKGLMRNE 391

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JVI35.4e-1022.89Transcription termination factor MTERF5, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9SZL61.7e-0827.08Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidop... [more]
F4IHL31.2e-0626.45Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9ZT968.0e-0626.76Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9FM801.5e-0425.83Transcription termination factor MTERF9, chloroplastic OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A0A0KUB96.4e-21696.31Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G036600 PE=3 SV=1[more]
A0A5A7TPC31.4e-17082.78Transcription termination factor MTERF5 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3BXT84.5e-16981.31transcription termination factor MTERF5, chloroplastic OS=Cucumis melo OX=3656 G... [more]
A0A5D3CYZ24.5e-16981.31Transcription termination factor MTERF5 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A6J1JB802.3e-14170.54uncharacterized protein LOC111482884 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
XP_004149163.24.1e-21799.49transcription termination factor MTERF5, chloroplastic [Cucumis sativus] >KAE863... [more]
KAA0044738.12.9e-17082.78transcription termination factor MTERF5 [Cucumis melo var. makuwa][more]
XP_008453751.19.3e-16981.31PREDICTED: transcription termination factor MTERF5, chloroplastic [Cucumis melo]... [more]
XP_031739970.18.4e-16293.42transcription termination factor MTERF5, chloroplastic-like [Cucumis sativus] >K... [more]
XP_038888519.15.9e-14773.01transcription termination factor MTERF5, chloroplastic-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G64950.11.5e-6037.68Mitochondrial transcription termination factor family protein [more]
AT1G21150.17.2e-3428.09Mitochondrial transcription termination factor family protein [more]
AT5G07900.18.7e-3226.18Mitochondrial transcription termination factor family protein [more]
AT1G62120.11.2e-2024.11Mitochondrial transcription termination factor family protein [more]
AT1G61970.15.9e-2023.81Mitochondrial transcription termination factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003690Transcription termination factor, mitochondrial/chloroplasticSMARTSM00733mt_12coord: 214..244
e-value: 130.0
score: 10.7
coord: 103..134
e-value: 0.01
score: 25.0
coord: 283..314
e-value: 170.0
score: 9.8
coord: 178..209
e-value: 0.62
score: 19.1
coord: 315..346
e-value: 240.0
score: 8.7
coord: 139..171
e-value: 51.0
score: 12.8
coord: 248..278
e-value: 1800.0
score: 1.8
IPR003690Transcription termination factor, mitochondrial/chloroplasticPFAMPF02536mTERFcoord: 259..367
e-value: 5.3E-13
score: 48.6
coord: 171..246
e-value: 2.7E-5
score: 23.2
coord: 90..166
e-value: 9.0E-11
score: 41.2
IPR003690Transcription termination factor, mitochondrial/chloroplasticPANTHERPTHR13068CGI-12 PROTEIN-RELATEDcoord: 32..384
IPR038538MTERF superfamily, mitochondrial/chloroplasticGENE3D1.25.70.10Transcription termination factor 3, mitochondrialcoord: 169..385
e-value: 4.0E-36
score: 126.5
NoneNo IPR availablePANTHERPTHR13068:SF173EMB|CAB62602.1coord: 32..384

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G04850.1CSPI04G04850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006353 DNA-templated transcription, termination
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003690 double-stranded DNA binding