CSPI04G04480 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G04480
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionTetratricopeptide-like helical
LocationChr4: 2914246 .. 2914647 (+)
RNA-Seq ExpressionCSPI04G04480
SyntenyCSPI04G04480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGGTTTCCTTGGAAGCTTTGGCAATGGCTGGGATTGATTCCAATGAGTGGGCTATGGACGCTGAAGAATGGGAAAGAAACGAGATCGACGTTGTTCCACCACATTTATTAGCCGATGAAGAAGATGACGAGGATGGTGATGAACATGTAACGACGAGTACTATTAGTAACTATTGCTTTCCCGTTGATGGGTCATTCGAGGGATGGCGTGGGAGAGATTGCAAAGCTGATGGTGGCCATGGCCATGGCATTTTGGGGATTTTTTGTAGTCTAAGCAAAGTCATAGAGAAAAGGGCTTCTAATGGGATGAGTTCAGTGAGAATAATAGTCAGAACTATTTTAGGGTTTTTGATGGTGATGACCATGAAGATAATTAGTGCACAAAGATCCAAGATGTAA

mRNA sequence

ATGTCGGTTTCCTTGGAAGCTTTGGCAATGGCTGGGATTGATTCCAATGAGTGGGCTATGGACGCTGAAGAATGGGAAAGAAACGAGATCGACGTTGTTCCACCACATTTATTAGCCGATGAAGAAGATGACGAGGATGGTGATGAACATGTAACGACGAGTACTATTAGTAACTATTGCTTTCCCGTTGATGGGTCATTCGAGGGATGGCGTGGGAGAGATTGCAAAGCTGATGGTGGCCATGGCCATGGCATTTTGGGGATTTTTTGTAGTCTAAGCAAAGTCATAGAGAAAAGGGCTTCTAATGGGATGAGTTCAGTGAGAATAATAGTCAGAACTATTTTAGGGTTTTTGATGGTGATGACCATGAAGATAATTAGTGCACAAAGATCCAAGATGTAA

Coding sequence (CDS)

ATGTCGGTTTCCTTGGAAGCTTTGGCAATGGCTGGGATTGATTCCAATGAGTGGGCTATGGACGCTGAAGAATGGGAAAGAAACGAGATCGACGTTGTTCCACCACATTTATTAGCCGATGAAGAAGATGACGAGGATGGTGATGAACATGTAACGACGAGTACTATTAGTAACTATTGCTTTCCCGTTGATGGGTCATTCGAGGGATGGCGTGGGAGAGATTGCAAAGCTGATGGTGGCCATGGCCATGGCATTTTGGGGATTTTTTGTAGTCTAAGCAAAGTCATAGAGAAAAGGGCTTCTAATGGGATGAGTTCAGTGAGAATAATAGTCAGAACTATTTTAGGGTTTTTGATGGTGATGACCATGAAGATAATTAGTGCACAAAGATCCAAGATGTAA

Protein sequence

MSVSLEALAMAGIDSNEWAMDAEEWERNEIDVVPPHLLADEEDDEDGDEHVTTSTISNYCFPVDGSFEGWRGRDCKADGGHGHGILGIFCSLSKVIEKRASNGMSSVRIIVRTILGFLMVMTMKIISAQRSKM*
Homology
BLAST of CSPI04G04480 vs. ExPASy TrEMBL
Match: A0A0A0KWE7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G026810 PE=4 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 2.0e-68
Identity = 133/133 (100.00%), Postives = 133/133 (100.00%), Query Frame = 0

Query: 1   MSVSLEALAMAGIDSNEWAMDAEEWERNEIDVVPPHLLADEEDDEDGDEHVTTSTISNYC 60
           MSVSLEALAMAGIDSNEWAMDAEEWERNEIDVVPPHLLADEEDDEDGDEHVTTSTISNYC
Sbjct: 1   MSVSLEALAMAGIDSNEWAMDAEEWERNEIDVVPPHLLADEEDDEDGDEHVTTSTISNYC 60

Query: 61  FPVDGSFEGWRGRDCKADGGHGHGILGIFCSLSKVIEKRASNGMSSVRIIVRTILGFLMV 120
           FPVDGSFEGWRGRDCKADGGHGHGILGIFCSLSKVIEKRASNGMSSVRIIVRTILGFLMV
Sbjct: 61  FPVDGSFEGWRGRDCKADGGHGHGILGIFCSLSKVIEKRASNGMSSVRIIVRTILGFLMV 120

Query: 121 MTMKIISAQRSKM 134
           MTMKIISAQRSKM
Sbjct: 121 MTMKIISAQRSKM 133

BLAST of CSPI04G04480 vs. ExPASy TrEMBL
Match: A0A5D3CZU0 (Tetratricopeptide-like helical OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00110 PE=4 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.2e-51
Identity = 115/133 (86.47%), Postives = 118/133 (88.72%), Query Frame = 0

Query: 1   MSVSLEALAMAGIDSNEWAMDAEEWERNEIDVVPPHLLADEEDDEDGDEHVTTSTISNYC 60
           MSVSLEALAMAGIDSNEW MDA+EWERNEIDVVPPHLLADEEDDEDG+    TSTISNYC
Sbjct: 1   MSVSLEALAMAGIDSNEWGMDAQEWERNEIDVVPPHLLADEEDDEDGE--YVTSTISNYC 60

Query: 61  FPVDGSFEGWRGRDCKADGGHGHGILGIFCSLSKVIEKRASNGMSSVRIIVRTILGFLMV 120
           F VDGSF GWR RD KADG     ILGIF SLSKVIEKRASNGM+SVRIIVRTILGFLMV
Sbjct: 61  FRVDGSFGGWRRRDWKADG--ALRILGIFYSLSKVIEKRASNGMNSVRIIVRTILGFLMV 120

Query: 121 MTMKIISAQRSKM 134
           MTMKIISAQRSKM
Sbjct: 121 MTMKIISAQRSKM 129

BLAST of CSPI04G04480 vs. ExPASy TrEMBL
Match: A0A5A7TS68 (Tetratricopeptide-like helical OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G004640 PE=4 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 5.7e-47
Identity = 105/122 (86.07%), Postives = 108/122 (88.52%), Query Frame = 0

Query: 1   MSVSLEALAMAGIDSNEWAMDAEEWERNEIDVVPPHLLADEEDDEDGDEHVTTSTISNYC 60
           MSVSLEALAMAGIDSNEW MDA+EWERNEIDVVPPHLLADEEDDEDG+    TSTISNYC
Sbjct: 1   MSVSLEALAMAGIDSNEWGMDAQEWERNEIDVVPPHLLADEEDDEDGE--YVTSTISNYC 60

Query: 61  FPVDGSFEGWRGRDCKADGGHGHGILGIFCSLSKVIEKRASNGMSSVRIIVRTILGFLMV 120
           F VDGSF GWR RD KADG   H ILGIF SLSKVIEKRASNGM+SVRIIVRTILGFLMV
Sbjct: 61  FRVDGSFGGWRRRDWKADG--AHRILGIFYSLSKVIEKRASNGMNSVRIIVRTILGFLMV 118

Query: 121 MT 123
           MT
Sbjct: 121 MT 118

BLAST of CSPI04G04480 vs. ExPASy TrEMBL
Match: A0A6J1FX98 (uncharacterized protein LOC111447880 OS=Cucurbita moschata OX=3662 GN=LOC111447880 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 3.7e-22
Identity = 70/139 (50.36%), Postives = 88/139 (63.31%), Query Frame = 0

Query: 1   MSVSLEALAMAGIDSNEWAMDAEEWERNEIDV-VPPHLLADEEDDEDGDEHVTTSTISNY 60
           MSVSLEALAMAGIDSNEWAMDAEEWERNE++  +PPHLLADE+D+ED             
Sbjct: 1   MSVSLEALAMAGIDSNEWAMDAEEWERNEMEKDIPPHLLADEDDEED------------- 60

Query: 61  CFPVDGSFEGWRGRDCKADGGHGHGILGIFCS------LSKVIEKRASNGMSSVRIIVRT 120
              +   F   R   C+ D G G    G+ CS      L K+ EKR  N +SS+ I+   
Sbjct: 61  --EMSTKFPATRCSCCRKDDGVG----GVSCSPTIMWHLRKITEKRDLNCLSSMEIVTSA 120

Query: 121 ILGFLMVMTMKIISAQRSK 133
           ++GFLM MT+KII+++RSK
Sbjct: 121 VVGFLMFMTIKIIASKRSK 120

BLAST of CSPI04G04480 vs. ExPASy TrEMBL
Match: A0A5N6Q1H5 (Uncharacterized protein OS=Mikania micrantha OX=192012 GN=E3N88_01585 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 2.3e-08
Identity = 38/72 (52.78%), Postives = 45/72 (62.50%), Query Frame = 0

Query: 1   MSVSLEALAMAGIDSNEWAMDAEEWERNEIDVVPPHLLAD----EEDDEDGDEHVTTSTI 60
           MS SLEALAMAG+D NEW MD EEWER ++   PPHL AD    +E D + DE V   T 
Sbjct: 95  MSFSLEALAMAGVDYNEWGMDLEEWERMKMGPPPPHLYADDYEEQEHDHERDEEVIMITA 154

Query: 61  SNYCFPVDGSFE 69
           +N    + GS E
Sbjct: 155 TNVTHELCGSKE 166

BLAST of CSPI04G04480 vs. NCBI nr
Match: TYK16880.1 (Tetratricopeptide-like helical [Cucumis melo var. makuwa])

HSP 1 Score: 211.5 bits (537), Expect = 4.6e-51
Identity = 115/133 (86.47%), Postives = 118/133 (88.72%), Query Frame = 0

Query: 1   MSVSLEALAMAGIDSNEWAMDAEEWERNEIDVVPPHLLADEEDDEDGDEHVTTSTISNYC 60
           MSVSLEALAMAGIDSNEW MDA+EWERNEIDVVPPHLLADEEDDEDG+    TSTISNYC
Sbjct: 1   MSVSLEALAMAGIDSNEWGMDAQEWERNEIDVVPPHLLADEEDDEDGE--YVTSTISNYC 60

Query: 61  FPVDGSFEGWRGRDCKADGGHGHGILGIFCSLSKVIEKRASNGMSSVRIIVRTILGFLMV 120
           F VDGSF GWR RD KADG     ILGIF SLSKVIEKRASNGM+SVRIIVRTILGFLMV
Sbjct: 61  FRVDGSFGGWRRRDWKADG--ALRILGIFYSLSKVIEKRASNGMNSVRIIVRTILGFLMV 120

Query: 121 MTMKIISAQRSKM 134
           MTMKIISAQRSKM
Sbjct: 121 MTMKIISAQRSKM 129

BLAST of CSPI04G04480 vs. NCBI nr
Match: KAA0044707.1 (Tetratricopeptide-like helical [Cucumis melo var. makuwa])

HSP 1 Score: 196.8 bits (499), Expect = 1.2e-46
Identity = 105/122 (86.07%), Postives = 108/122 (88.52%), Query Frame = 0

Query: 1   MSVSLEALAMAGIDSNEWAMDAEEWERNEIDVVPPHLLADEEDDEDGDEHVTTSTISNYC 60
           MSVSLEALAMAGIDSNEW MDA+EWERNEIDVVPPHLLADEEDDEDG+    TSTISNYC
Sbjct: 1   MSVSLEALAMAGIDSNEWGMDAQEWERNEIDVVPPHLLADEEDDEDGE--YVTSTISNYC 60

Query: 61  FPVDGSFEGWRGRDCKADGGHGHGILGIFCSLSKVIEKRASNGMSSVRIIVRTILGFLMV 120
           F VDGSF GWR RD KADG   H ILGIF SLSKVIEKRASNGM+SVRIIVRTILGFLMV
Sbjct: 61  FRVDGSFGGWRRRDWKADG--AHRILGIFYSLSKVIEKRASNGMNSVRIIVRTILGFLMV 118

Query: 121 MT 123
           MT
Sbjct: 121 MT 118

BLAST of CSPI04G04480 vs. NCBI nr
Match: KAG6577295.1 (hypothetical protein SDJN03_24869, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 161.8 bits (408), Expect = 4.2e-36
Identity = 94/135 (69.63%), Postives = 102/135 (75.56%), Query Frame = 0

Query: 1   MSVSLEALAMAGIDSNEWAMDAEEWERNEID-VVPPHLLADEEDDEDGDEHVTTSTISNY 60
           MSVSLEALAMAGI SNEWAMDAEEWERNEI+  VPPHLLADE+DD+D      TST+SNY
Sbjct: 1   MSVSLEALAMAGITSNEWAMDAEEWERNEIENDVPPHLLADEDDDDDN----VTSTVSNY 60

Query: 61  CFPVDGSFEGWRGRDCKADGGHG-HGILGIFCSLSKVIEKRASNGMSSVRIIVRTILGFL 120
            FPV G F+  R  D    G H  H   GI CSLSK +EKRASNGMSSVRIIVRTILGFL
Sbjct: 61  SFPVAGFFDSLRRED---GGAHDIHCFWGILCSLSKSLEKRASNGMSSVRIIVRTILGFL 120

Query: 121 MVMTMKIISAQRSKM 134
           +V  +KIISA  S M
Sbjct: 121 LVKILKIISAHTSNM 128

BLAST of CSPI04G04480 vs. NCBI nr
Match: KAG7015383.1 (hypothetical protein SDJN02_23018, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 161.0 bits (406), Expect = 7.1e-36
Identity = 93/134 (69.40%), Postives = 101/134 (75.37%), Query Frame = 0

Query: 1   MSVSLEALAMAGIDSNEWAMDAEEWERNEID-VVPPHLLADEEDDEDGDEHVTTSTISNY 60
           MSVSLEALAMAGI SNEWAMDAEEWERNEI+  VPPHLLADE+DD+D      TST+SNY
Sbjct: 1   MSVSLEALAMAGITSNEWAMDAEEWERNEIENDVPPHLLADEDDDDDN----VTSTVSNY 60

Query: 61  CFPVDGSFEGWRGRDCKADGGHGHGILGIFCSLSKVIEKRASNGMSSVRIIVRTILGFLM 120
            FPV G F+     D  AD    H   GI CSLSK +EKRASNGMSSVRIIVRTILGFL+
Sbjct: 61  SFPVAGFFDSLHREDGGAD--DIHCFWGILCSLSKSLEKRASNGMSSVRIIVRTILGFLL 120

Query: 121 VMTMKIISAQRSKM 134
           V  +KIISA  S M
Sbjct: 121 VKILKIISAHTSNM 128

BLAST of CSPI04G04480 vs. NCBI nr
Match: XP_022943015.1 (uncharacterized protein LOC111447880 [Cucurbita moschata])

HSP 1 Score: 114.4 bits (285), Expect = 7.7e-22
Identity = 70/139 (50.36%), Postives = 88/139 (63.31%), Query Frame = 0

Query: 1   MSVSLEALAMAGIDSNEWAMDAEEWERNEIDV-VPPHLLADEEDDEDGDEHVTTSTISNY 60
           MSVSLEALAMAGIDSNEWAMDAEEWERNE++  +PPHLLADE+D+ED             
Sbjct: 1   MSVSLEALAMAGIDSNEWAMDAEEWERNEMEKDIPPHLLADEDDEED------------- 60

Query: 61  CFPVDGSFEGWRGRDCKADGGHGHGILGIFCS------LSKVIEKRASNGMSSVRIIVRT 120
              +   F   R   C+ D G G    G+ CS      L K+ EKR  N +SS+ I+   
Sbjct: 61  --EMSTKFPATRCSCCRKDDGVG----GVSCSPTIMWHLRKITEKRDLNCLSSMEIVTSA 120

Query: 121 ILGFLMVMTMKIISAQRSK 133
           ++GFLM MT+KII+++RSK
Sbjct: 121 VVGFLMFMTIKIIASKRSK 120

BLAST of CSPI04G04480 vs. TAIR 10
Match: AT2G22320.1 (unknown protein; Has 7 Blast hits to 7 proteins in 4 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 7; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 49.3 bits (116), Expect = 2.8e-06
Identity = 28/52 (53.85%), Postives = 33/52 (63.46%), Query Frame = 0

Query: 5  LEALAMAGIDSNEWAMDAEEWERNEIDVVPPHLLAD-------EEDDEDGDE 50
          +EALAMAG+D  EW +  EEWE  E  VVP HLLAD       EED +D D+
Sbjct: 1  MEALAMAGVDYQEWGLSIEEWELQE-SVVPLHLLADDFKEEEQEEDHQDNDD 51

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0KWE72.0e-68100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G026810 PE=4 SV=1[more]
A0A5D3CZU02.2e-5186.47Tetratricopeptide-like helical OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A5A7TS685.7e-4786.07Tetratricopeptide-like helical OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A6J1FX983.7e-2250.36uncharacterized protein LOC111447880 OS=Cucurbita moschata OX=3662 GN=LOC1114478... [more]
A0A5N6Q1H52.3e-0852.78Uncharacterized protein OS=Mikania micrantha OX=192012 GN=E3N88_01585 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
TYK16880.14.6e-5186.47Tetratricopeptide-like helical [Cucumis melo var. makuwa][more]
KAA0044707.11.2e-4686.07Tetratricopeptide-like helical [Cucumis melo var. makuwa][more]
KAG6577295.14.2e-3669.63hypothetical protein SDJN03_24869, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7015383.17.1e-3669.40hypothetical protein SDJN02_23018, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022943015.17.7e-2250.36uncharacterized protein LOC111447880 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G22320.12.8e-0653.85unknown protein; Has 7 Blast hits to 7 proteins in 4 species: Archae - 0; Bacter... [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G04480.1CSPI04G04480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane