CSPI04G04220 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G04220
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionpre-mRNA-splicing factor CWC25 homolog
LocationChr4: 2752024 .. 2754350 (-)
RNA-Seq ExpressionCSPI04G04220
SyntenyCSPI04G04220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGAAAAATAATTGTTTTCGTAATTTTGTTTTTAGATTTGAAAATCGGATTCTTCAACACAAAACCACACCGCCGGTTGCTGAAAATCCATTATCCGATCCCTAATCTGAAGCTCTGTTACAGACATGGCGTTGAAGTTCTTGAACAAGAAAGGATGGCATACTGGCAGTCTCCGGAACATAGAGAACGTATGGAAGGCGGAGCAAAAGCATGAGGCCGAACAGAAGAAGTTGGACGAGCTCCGCAAACAGATCCTCGAGGAGAAAGAACGCCAGGAGTTTCGTCTTCTTCAAGAACAAGCCGGCCTCGTTCCGTATGTTTCATCCTCTGTCTTTTTATTTTATTTGTGTTCTGTGAATTTCAAAATCTGATTCGATTTCTATGGGGATTGAAACCGTTCGTAGATTAGGGTTTGTGCCAAGGCGATGGCTAGGGTTTAATTAGTTCGATTGATTTGACCAATATGCAGGAAGCAGGAGAGATTGGATTTTCTTTATGAATCGGGTTTGGCAGTTGGGAAAGCTAGCAGTTCCGATGGATTTAAAAGCCTTGAAACCCTACCTAGTTCATCTACCGCCGCAGCTGCCACTGAACCATCTAGTTCCAAGGTACTGTTTCGTTTTTTCGTTGATTTGTGTGCTTTGGCTATCACTTTGTATGCTCAATTATGATGTTTGTTGGATTCTTGTGTTGTTTATGCAGGAAGCCGCTGTCCCTGGAGCTCTGTTTGAGGATAAGCCTCATTCTGCCAATGATACATGGAGGAAACTCCATTCTGATCCTTTGCTCATCATTCGACAGCGTGAGCAACAAGCTCTTGCTCGTGTTAAAAACAATCCTATTCAGATGGCTATGATTCGCAAAACTGTAAGTGTTTTTTAATCTCCTTATTGTTACAACATGGGCTGTGGAAATTTTGTGGAAACATCTTTCTTCCTTTCATTATTTTGTAGAGAGATTTTCTGAACGTCAACTGTTAGTTTGTTCATGGACACTTTACTTTGTGGTTGAGAAATCCGGTGGCCTATCTAAGTTCTAAGTTTTTCCTTTTGAGTTGATGTGATGGATTTCTCTTCATTTCTCTCACCCTTTTGATAGGAGCTAAAGATGAATCTTTGATCAGAAAGTTTCTTTCTATATATCTTCATGGCTAGTCACTTGTGGCAAAAGGACAAATGACGAACTGCCATGTTAAAGAATGTTCTTGTTGAGTTGTGATTGAATCTCAATTTAGTTCACTATCGTATCATGTTGAAAGCTCTCTTGTTTGATTTGAAAAATAGATTTTGAGGGTTTGTTTTTTAATTATTTCTTCATATATATAATCATAGGTTGAAGTGGAAAAACACAAGGATAAAAATCCTGATGACAAGAGAGAACGGAAGAAGCACCGCCATAGTAAGTCTAAGCGCCATAAAGATTCTTCTCCAGAAAGAGATTATGATTCTGAAGATGTCAGCCCTGAAAGGCAGAGAAGTAAACATGATCATGACAAGAGTTCAAGACATGATGGCCATTCTCATTCAGAAGATAGAAGAAGCAAAGCAGATACTAAGAACGAGAGGGACCGTGATAGAGGCTCAAAATATGCTGCACGAACAAGCTATGATCAGTCAGATCGAAAGACATTCAAAAGCAATCCTCATGATTCTGCAGCAGATAGACATCATGACAGATCAAAGAGAGATAGAGATTCTTATGCTAACAATGATAGGGACAGCAGAAGAGTGGGGGAACCAAGATATTATGAATCCAATGCTTCTGAAACTCCTAACGAGTCACGCCACAGGCATCGCAGGCCTACCACAAAGCTTTCAGAAGAAGAGCGTGCTGCTAGACTGCGTGAAATGCAGCAAGATGCCGAGTTACATGAAGAGCAACGATTCAAGCGTTTGAAGAAGGCAGATGAGGATGATGCACTGGAAGCCAAACAAAATGCAGTGCCCTCGAGTCGAAATTTCTTGGACATGGCTCAGAAACGCATGTACGGTGCTGAGAAGGGAGGAAGCTCAACGATCGAGGAGAGTATTCGCCGTAGGACATATTATTCACAGGGTAAATCTCAGATTGAAGCCAATGCTTTTAGACGGTAATCAACATCACTTGTATACTTCTGCTTGTGGTTAGTGTATAATTTGCCTTTTGAAGAATTGCTTTTTCATATATCAATGTGATCTGATCAGATTTGGGGTTTGTGATGGTTAGATTCATATATGGTTTGTTTTAATTAAATAGTTGTATTTTCTATTGATATTGATATATATTATCAACTTGTCTAACATTTCTTCTTCCTTATTATGTGAAAATTGTGACCTTGTTTGCTCTTA

mRNA sequence

AAGAAAAATAATTGTTTTCGTAATTTTGTTTTTAGATTTGAAAATCGGATTCTTCAACACAAAACCACACCGCCGGTTGCTGAAAATCCATTATCCGATCCCTAATCTGAAGCTCTGTTACAGACATGGCGTTGAAGTTCTTGAACAAGAAAGGATGGCATACTGGCAGTCTCCGGAACATAGAGAACGTATGGAAGGCGGAGCAAAAGCATGAGGCCGAACAGAAGAAGTTGGACGAGCTCCGCAAACAGATCCTCGAGGAGAAAGAACGCCAGGAGTTTCGTCTTCTTCAAGAACAAGCCGGCCTCGTTCCGAAGCAGGAGAGATTGGATTTTCTTTATGAATCGGGTTTGGCAGTTGGGAAAGCTAGCAGTTCCGATGGATTTAAAAGCCTTGAAACCCTACCTAGTTCATCTACCGCCGCAGCTGCCACTGAACCATCTAGTTCCAAGGAAGCCGCTGTCCCTGGAGCTCTGTTTGAGGATAAGCCTCATTCTGCCAATGATACATGGAGGAAACTCCATTCTGATCCTTTGCTCATCATTCGACAGCGTGAGCAACAAGCTCTTGCTCGTGTTAAAAACAATCCTATTCAGATGGCTATGATTCGCAAAACTGTTGAAGTGGAAAAACACAAGGATAAAAATCCTGATGACAAGAGAGAACGGAAGAAGCACCGCCATAGTAAGTCTAAGCGCCATAAAGATTCTTCTCCAGAAAGAGATTATGATTCTGAAGATGTCAGCCCTGAAAGGCAGAGAAGTAAACATGATCATGACAAGAGTTCAAGACATGATGGCCATTCTCATTCAGAAGATAGAAGAAGCAAAGCAGATACTAAGAACGAGAGGGACCGTGATAGAGGCTCAAAATATGCTGCACGAACAAGCTATGATCAGTCAGATCGAAAGACATTCAAAAGCAATCCTCATGATTCTGCAGCAGATAGACATCATGACAGATCAAAGAGAGATAGAGATTCTTATGCTAACAATGATAGGGACAGCAGAAGAGTGGGGGAACCAAGATATTATGAATCCAATGCTTCTGAAACTCCTAACGAGTCACGCCACAGGCATCGCAGGCCTACCACAAAGCTTTCAGAAGAAGAGCGTGCTGCTAGACTGCGTGAAATGCAGCAAGATGCCGAGTTACATGAAGAGCAACGATTCAAGCGTTTGAAGAAGGCAGATGAGGATGATGCACTGGAAGCCAAACAAAATGCAGTGCCCTCGAGTCGAAATTTCTTGGACATGGCTCAGAAACGCATGTACGGTGCTGAGAAGGGAGGAAGCTCAACGATCGAGGAGAGTATTCGCCGTAGGACATATTATTCACAGGGTAAATCTCAGATTGAAGCCAATGCTTTTAGACGGTAATCAACATCACTTGTATACTTCTGCTTGTGGTTAGTGTATAATTTGCCTTTTGAAGAATTGCTTTTTCATATATCAATGTGATCTGATCAGATTTGGGGTTTGTGATGGTTAGATTCATATATGGTTTGTTTTAATTAAATAGTTGTATTTTCTATTGATATTGATATATATTATCAACTTGTCTAACATTTCTTCTTCCTTATTATGTGAAAATTGTGACCTTGTTTGCTCTTA

Coding sequence (CDS)

ATGGCGTTGAAGTTCTTGAACAAGAAAGGATGGCATACTGGCAGTCTCCGGAACATAGAGAACGTATGGAAGGCGGAGCAAAAGCATGAGGCCGAACAGAAGAAGTTGGACGAGCTCCGCAAACAGATCCTCGAGGAGAAAGAACGCCAGGAGTTTCGTCTTCTTCAAGAACAAGCCGGCCTCGTTCCGAAGCAGGAGAGATTGGATTTTCTTTATGAATCGGGTTTGGCAGTTGGGAAAGCTAGCAGTTCCGATGGATTTAAAAGCCTTGAAACCCTACCTAGTTCATCTACCGCCGCAGCTGCCACTGAACCATCTAGTTCCAAGGAAGCCGCTGTCCCTGGAGCTCTGTTTGAGGATAAGCCTCATTCTGCCAATGATACATGGAGGAAACTCCATTCTGATCCTTTGCTCATCATTCGACAGCGTGAGCAACAAGCTCTTGCTCGTGTTAAAAACAATCCTATTCAGATGGCTATGATTCGCAAAACTGTTGAAGTGGAAAAACACAAGGATAAAAATCCTGATGACAAGAGAGAACGGAAGAAGCACCGCCATAGTAAGTCTAAGCGCCATAAAGATTCTTCTCCAGAAAGAGATTATGATTCTGAAGATGTCAGCCCTGAAAGGCAGAGAAGTAAACATGATCATGACAAGAGTTCAAGACATGATGGCCATTCTCATTCAGAAGATAGAAGAAGCAAAGCAGATACTAAGAACGAGAGGGACCGTGATAGAGGCTCAAAATATGCTGCACGAACAAGCTATGATCAGTCAGATCGAAAGACATTCAAAAGCAATCCTCATGATTCTGCAGCAGATAGACATCATGACAGATCAAAGAGAGATAGAGATTCTTATGCTAACAATGATAGGGACAGCAGAAGAGTGGGGGAACCAAGATATTATGAATCCAATGCTTCTGAAACTCCTAACGAGTCACGCCACAGGCATCGCAGGCCTACCACAAAGCTTTCAGAAGAAGAGCGTGCTGCTAGACTGCGTGAAATGCAGCAAGATGCCGAGTTACATGAAGAGCAACGATTCAAGCGTTTGAAGAAGGCAGATGAGGATGATGCACTGGAAGCCAAACAAAATGCAGTGCCCTCGAGTCGAAATTTCTTGGACATGGCTCAGAAACGCATGTACGGTGCTGAGAAGGGAGGAAGCTCAACGATCGAGGAGAGTATTCGCCGTAGGACATATTATTCACAGGGTAAATCTCAGATTGAAGCCAATGCTTTTAGACGGTAA

Protein sequence

MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAGLVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFEDKPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRERKKHRHSKSKRHKDSSPERDYDSEDVSPERQRSKHDHDKSSRHDGHSHSEDRRSKADTKNERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRHHDRSKRDRDSYANNDRDSRRVGEPRYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADEDDALEAKQNAVPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR*
Homology
BLAST of CSPI04G04220 vs. ExPASy Swiss-Prot
Match: Q9NXE8 (Pre-mRNA-splicing factor CWC25 homolog OS=Homo sapiens OX=9606 GN=CWC25 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 3.7e-17
Identity = 129/441 (29.25%), Postives = 216/441 (48.98%), Query Frame = 0

Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAGLV-PKQE 67
           KK WH  +LRN+E VWKAEQKHEAE+KK++EL++++ EE+ R+E +   E  G V  K+E
Sbjct: 9   KKSWHPQTLRNVEKVWKAEQKHEAERKKIEELQRELREERAREEMQRYAEDVGAVKKKEE 68

Query: 68  RLDFLYE--SGLA------VGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALF 127
           +LD++Y+   G+       +G+      F+ +E   +           SS+   +PG++F
Sbjct: 69  KLDWMYQGPGGMVNRDEYLLGRPIDKYVFEKMEEKEAG---------CSSETGLLPGSIF 128

Query: 128 EDKPHSAN---DTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNP 187
              P  AN   D   K+  DPL IIR++E++    V NNP++M  I++ +++   K +  
Sbjct: 129 --APSGANSLLDMASKIREDPLFIIRKKEEEKKREVLNNPVKMKKIKELLQMSLEKKEKK 188

Query: 188 DDKRERKKHRHSKSKRHKDSSPERDYDSEDVSPERQRSKHDHDKS--SRHDGHS---HSE 247
             K ++KKH   K  +H+ SS +R    ++ S  R + K  +     S+  G+     + 
Sbjct: 189 KKKEKKKKH---KKHKHRSSSSDRSSSEDEHSAGRSQKKMANSSPVLSKVPGYGLQVRNS 248

Query: 248 DRRS--KADTKNERDRDRGSKYAAR---TSYDQSDRKTFKSNPHDSAADRHH----DRSK 307
           DR    +     E+ R  G K  +R   +S+      + KS     + DR       RS+
Sbjct: 249 DRNQGLQGPLTAEQKRGHGMKNHSRSRSSSHSPPRHASKKSTREAGSRDRRSRSLGRRSR 308

Query: 308 RDRDSYANNDRDSRR-VGEPRYYESNASETPNESRHRHRRP---TTKLSEEERAARLREM 367
             R S  +N + +RR  G+ R      S +P +  ++ R     T KLS EE   + +EM
Sbjct: 309 SPRPSKLHNSKVNRRETGQTR------SPSPKKEVYQRRHAPGYTRKLSAEELERKRQEM 368

Query: 368 QQDAELHEEQRFKRLKKADEDDALEAKQNAVPSSRNFLDMAQKRMYGAEKGGSSTIEESI 418
            ++A+  EE+R   LK+  +D+  E +   + S         K     E   +S++E+ +
Sbjct: 369 MENAKWREEERLNILKRHAKDEEREQRLEKLDSRDGKFIHRMK----LESASTSSLEDRV 425

BLAST of CSPI04G04220 vs. ExPASy Swiss-Prot
Match: Q9DBF7 (Pre-mRNA-splicing factor CWC25 homolog OS=Mus musculus OX=10090 GN=Cwc25 PE=2 SV=2)

HSP 1 Score: 90.9 bits (224), Expect = 3.7e-17
Identity = 126/436 (28.90%), Postives = 210/436 (48.17%), Query Frame = 0

Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAGLV-PKQE 67
           KK WH  +LRN+E VWKAEQKHEAE+KK++EL++++ EE+ R+E +   E  G V  K+E
Sbjct: 9   KKSWHPQTLRNVEKVWKAEQKHEAERKKIEELQRELREERAREEMQRYAEDVGAVKKKEE 68

Query: 68  RLDFLYE--SGLA------VGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALF 127
           +LD++Y+   G+       +G+      F+ +E   +           SS+   +PG++F
Sbjct: 69  KLDWMYQGPGGMVNRDEYLLGRPIDKYVFEKMEEREAG---------CSSETGLLPGSIF 128

Query: 128 EDKPHSAN---DTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNP 187
              P  AN   D   K+  DPL IIR++E++    V NNP++M  I++ +++   K +  
Sbjct: 129 --APSGANSLLDMASKIREDPLFIIRKKEEEKKREVLNNPVKMKKIKELLQMSLEKKEKK 188

Query: 188 DDKRERKKHRHSKSKRHKDSSPERDYDSEDVSPERQRSK--HDHDKSSRHDGHSHSEDRR 247
             K ++KKHR  K   H+ SS       ++ S  R + K  +     S+  G+       
Sbjct: 189 KKKEKKKKHRKHK---HRSSSSGGSSSEDEQSQARSQKKMANSFPVLSKVPGYGLQ---- 248

Query: 248 SKADTKNERDRDRG--SKYAARTSYDQSDRKTFKSNPHDSAADRHHDRSKRDRDSYANND 307
                  + DR+RG       + +   + R    S P  ++     +   RDR S + + 
Sbjct: 249 -----VRDSDRNRGLQGSLGEQRAIKNNSRSRSSSPPRHASKKSTKEERPRDRRSRSPSR 308

Query: 308 RD-SRRVGEPRYYESNASE----TPNESRHRHRRP---TTKLSEEERAARLREMQQDAEL 367
           R  S R  +P   + N  E    +P +  ++ R     T KLS EE   + +EM ++A+ 
Sbjct: 309 RSRSPRPSKPHTSKVNRKERDSPSPKKEAYQRRHASGYTRKLSAEELERKRQEMMENAKW 368

Query: 368 HEEQRFKRLKKADEDDALEAKQNAVPS-SRNFLDMAQKRMYGAEKGGSSTIEESIRRRTY 418
            EE+R   LK+   +D  E +   + S S  FL   +      E   +S++E+ ++R  +
Sbjct: 369 REEERLNTLKRHAREDEREHRLERLDSRSGKFLHRMK-----LESASTSSLEDRVKRNIH 416

BLAST of CSPI04G04220 vs. ExPASy Swiss-Prot
Match: Q5B0I1 (Pre-mRNA-splicing factor cwc25 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=cwc25 PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.4e-11
Identity = 117/394 (29.70%), Postives = 183/394 (46.45%), Query Frame = 0

Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAGLVPKQER 67
           KK WH   LRN E VW  E++   E+K++D+L+++  EE++ QE + LQE AG   + +R
Sbjct: 8   KKSWHPSLLRNQERVWAEEKRALEERKRIDQLKRERDEERQIQELQRLQESAGGTRQLQR 67

Query: 68  LDFLYE-------------SGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVP 127
           +D++Y+              G  +GK    DG              AAT P ++  A +P
Sbjct: 68  VDWMYQEPSSASGHYAEEMEGYLLGK-RRIDGILLKNDESKKLEKGAATGPGAA--AGLP 127

Query: 128 GALFEDKPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTV---EVEKHK 187
             +     H+  DT  K+ +DPLL IR+REQ A           AM++++V   E E+ +
Sbjct: 128 PVV-----HNTRDTMAKVMADPLLEIRKREQAAYE---------AMVKESVRRREREQGR 187

Query: 188 DKNPDDKRERKKHRH--------SKSKRHKDSSPERDYDSEDVSPERQRSKHDHDKSSRH 247
           +++ + +RER  HRH         +S RH+  S  R         +R+    +HD+ S  
Sbjct: 188 ERHQERERER-GHRHRRDSGNDSHRSHRHRRRSRSRSRSPRVEKDDRRHRDREHDRRSHR 247

Query: 248 DGHSHSEDRRSKADTKNERDRDRGSKY---AARTSYDQSDRKTFKSNPHDSAADRHHDRS 307
           D     E+R  +    + RD DR  +Y   A R++  + DR+       D   D   DR+
Sbjct: 248 DRDGERENRVERVYHSSRRDHDRDERYDRAARRSASPRPDRRRDDRRDRDQDQDYRRDRN 307

Query: 308 KRD--RDSYANNDRDSRRVGEPRYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQ 367
            R   RD+ ++ DRD+   G  R      S + N +   +     +  EE+R  RL EMQ
Sbjct: 308 SRSSRRDARSSRDRDTDAFGRDR-NRDRGSHSRNGNADANADQHAQELEEQRKRRLAEMQ 367

Query: 368 QDAELHEEQRFKRLKKADEDDALEAKQNAVPSSR 373
            +A+  E  R +R+    E  ALE KQ      R
Sbjct: 368 ANAQDMEATRRQRVA---EVTALEEKQREEDDKR 379

BLAST of CSPI04G04220 vs. ExPASy Swiss-Prot
Match: Q4P6I3 (Pre-mRNA-splicing factor CWC25 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=CWC25 PE=3 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 4.4e-10
Identity = 110/402 (27.36%), Postives = 191/402 (47.51%), Query Frame = 0

Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAGLVPKQER 67
           KK WH     N E VWK E++   E+KKL+ELR++  +E+E Q+ + LQE+AG   + +R
Sbjct: 9   KKSWHPLLQVNQERVWKREKEALEERKKLEELRREREQEREMQQLQRLQEEAGGKKRIDR 68

Query: 68  LDFLYESGLAVGKASSSD------GFKSLETLPSSSTAAAATEPSSSKEAAVPGALFEDK 127
           +D++Y +    G  S+++      G K ++ L         ++ +     ++  A     
Sbjct: 69  VDWMYATPATTGSGSAAEMEDYLLGKKRVDKLLQGEENKEMSKTTQKSLVSLQNA----- 128

Query: 128 PHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKR-- 187
            +SA D   K+  DP+L I+Q+EQ A   +  +P ++  ++    ++       + +R  
Sbjct: 129 -NSARDLAAKIREDPMLAIKQQEQAAYEALLRDPTRLRQLKMQAGIDTDTQSKDERRRSK 188

Query: 188 ERKKHRHSKSK------RHKDSSPERDYDSEDVSPERQRSKHDHDKSSRHDGHSHSEDRR 247
           E K+ RH + +      RH +S  E    S     +R+ S ++   S RHDG    E+RR
Sbjct: 189 EEKRRRHDRDEHRRRGSRHGESRSESRSHSRRDDRDRKSSWNEGSSSRRHDG---DEERR 248

Query: 248 SKADTKNERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRHHDRSKRDRDSYANN--- 307
           S    ++++DRDR  +Y  R       R   +SN      D   + S R   SY+ +   
Sbjct: 249 SLKRHRDDQDRDR-HRYERRLHDADDSRPASRSNRDRRDKDAVDESSSRQSTSYSRSRSR 308

Query: 308 ---DRDSRRVGEPRYYESNASETP-NESRHRHRRPTTKLSEEERAARLREMQQDAELHEE 367
               RD R   + R   S+A   P N    R+R    +  ++ R  +L+EM+Q+A   E+
Sbjct: 309 SPPARDYR--NDKRLSSSSAFTRPSNGGSARNREEDEERKQQARQLKLQEMEQNARSMEQ 368

Query: 368 QRFKRLKKADEDDALEAKQNAVPSSRNFLDMAQKRMYGAEKG 389
           QR   + K + ++A + ++ A    R  L  A+ + +G  KG
Sbjct: 369 QRSSYVSKINAEEAEQERREA--ELRQKLIDARSKGHGDGKG 396

BLAST of CSPI04G04220 vs. ExPASy Swiss-Prot
Match: Q4X1D7 (Pre-mRNA-splicing factor cwc25 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=cwc25 PE=3 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.3e-09
Identity = 123/446 (27.58%), Postives = 202/446 (45.29%), Query Frame = 0

Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAGLVPKQER 67
           KK WH   LRN E VW  E++   E+K++++LR++  EE++ QE + LQE +G   +  R
Sbjct: 8   KKSWHPSLLRNQERVWAEEKRALEERKRIEQLRREREEERQIQELQRLQEASGKGRQLNR 67

Query: 68  LDFLYES-GLAVGK-ASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFEDKP-HS 127
           +D++Y++   A G  A   +G+   +                 K   V GA     P ++
Sbjct: 68  VDWMYQAPSSATGHYAEEMEGYLLGKRRIDGILLKNDESKKLEKGTDVVGANAAPPPVNN 127

Query: 128 ANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKH-KDKNPDDKRERKK 187
             DT  K+ +DPLL I++REQ A            M++++V   +H +DK+ D  RER++
Sbjct: 128 PRDTMAKVMADPLLEIKKREQAAYEN---------MVKESVRRSQHIRDKDRDRDRERRR 187

Query: 188 HRHSKSK-----------RHKDSSPERDY--------DSEDVSPERQRSKHDHDKSSR-- 247
            R  + +           RH+D    R +            +SP+R   K   D+  R  
Sbjct: 188 DRGDRDRSRDRDRSHRRSRHEDEDAHRSHRHRSHRHRSRSPLSPDRSDRKRGDDRDYRDE 247

Query: 248 ----------HDGHSHSEDRRSKADTKNER-DRDR-GSKYAARTS-YDQSDRKTFKS--N 307
                     H GH   +DRRS+ D + +R D DR  S+Y  R   YD S R+   +  +
Sbjct: 248 RDRRDDRRDEHRGHDRRDDRRSERDRRADRGDEDRHSSRYKDRDDRYDHSTRRRSYADRS 307

Query: 308 PHDSAADRHHDRSKRDRDSYANNDRDSRRVGEPRYYESN---ASETPNESRHRHRRPTT- 367
           P     + ++DR +R  D      RDSR    P +Y  N    ++ P +S  R R   T 
Sbjct: 308 PSPRRGNHYYDR-RRTEDRSDGYHRDSRNHDRPEFYRGNDRKVNKEPRDSNLRERSSNTR 367

Query: 368 --------KLSEEERAARLREMQQDAELHEEQRFKRLKK--ADEDDALEAKQNAVPSSRN 400
                   K+ EEER  +L EM  +A+  E++R +R+ +  A E+   EA +        
Sbjct: 368 DEVAGNKEKVLEEERKKKLAEMMSNADELEQKRLQRIAEVTAMEEKEREADEKQRSERGR 427

BLAST of CSPI04G04220 vs. ExPASy TrEMBL
Match: A0A0A0KU62 (Cir_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G025080 PE=3 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 2.1e-212
Identity = 411/417 (98.56%), Postives = 413/417 (99.04%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120
           LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180
           KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE
Sbjct: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180

Query: 181 RKKHRHSKSKRHKDSSPERDYDSEDVSPERQRSKHDHDKSSRHDGHSHSEDRRSKADTKN 240
           RKKHRHSKSKRHKDSSPERDYDSEDVSPERQR KHDHDKSSRHDGHSHSEDRRSKA+TKN
Sbjct: 181 RKKHRHSKSKRHKDSSPERDYDSEDVSPERQRRKHDHDKSSRHDGHSHSEDRRSKAETKN 240

Query: 241 ERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRHHDRSKRDRDSYANNDRDSRRVGEP 300
           ERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADR+HDRSKRDRDSYA NDRDSRRVGE 
Sbjct: 241 ERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRYHDRSKRDRDSYATNDRDSRRVGEL 300

Query: 301 RYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADEDDA 360
           RYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADEDDA
Sbjct: 301 RYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADEDDA 360

Query: 361 LEAKQNAVPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR 418
           LEAKQNAVPSSRNFLDMAQKRMY AEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR
Sbjct: 361 LEAKQNAVPSSRNFLDMAQKRMYSAEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR 417

BLAST of CSPI04G04220 vs. ExPASy TrEMBL
Match: A0A5A7TSZ0 (Pre-mRNA-splicing factor CWC25-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00360 PE=3 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 9.9e-207
Identity = 403/419 (96.18%), Postives = 409/419 (97.61%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120
           LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAA PGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAAPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180
           KPHSAND WRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE
Sbjct: 121 KPHSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180

Query: 181 RKKHRHSKSKRHKDSSPERDYDSEDVSPERQRSKHDHDKSSRHDGHSHSEDRRSKADTKN 240
           RKKHRHSKSKRHKDSSPERDYDSED SPERQR KHDHDKSSRHDGHSHSEDRRSKAD+KN
Sbjct: 181 RKKHRHSKSKRHKDSSPERDYDSEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 240

Query: 241 ERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRHHDRSK--RDRDSYANNDRDSRRVG 300
           ERDRDRGSKYAARTSYDQSDRKT KSNP DSAADRHHDRSK  RDRDS+ANND+DSRRVG
Sbjct: 241 ERDRDRGSKYAARTSYDQSDRKTSKSNPRDSAADRHHDRSKRDRDRDSHANNDKDSRRVG 300

Query: 301 EPRYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADED 360
           EPRYYES+ASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQR+KRLKKA ED
Sbjct: 301 EPRYYESHASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAED 360

Query: 361 DALEAKQNAVPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR 418
           DALEAKQNAVPSSRNFLD AQKRMYGAEKGGSSTIEESIRRRTYYSQGKS+IEANAFRR
Sbjct: 361 DALEAKQNAVPSSRNFLDTAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 419

BLAST of CSPI04G04220 vs. ExPASy TrEMBL
Match: A0A1S3BY15 (pre-mRNA-splicing factor CWC25 homolog OS=Cucumis melo OX=3656 GN=LOC103494454 PE=3 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 9.9e-207
Identity = 403/419 (96.18%), Postives = 409/419 (97.61%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120
           LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAA PGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAAPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180
           KPHSAND WRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE
Sbjct: 121 KPHSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180

Query: 181 RKKHRHSKSKRHKDSSPERDYDSEDVSPERQRSKHDHDKSSRHDGHSHSEDRRSKADTKN 240
           RKKHRHSKSKRHKDSSPERDYDSED SPERQR KHDHDKSSRHDGHSHSEDRRSKAD+KN
Sbjct: 181 RKKHRHSKSKRHKDSSPERDYDSEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 240

Query: 241 ERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRHHDRSK--RDRDSYANNDRDSRRVG 300
           ERDRDRGSKYAARTSYDQSDRKT KSNP DSAADRHHDRSK  RDRDS+ANND+DSRRVG
Sbjct: 241 ERDRDRGSKYAARTSYDQSDRKTSKSNPRDSAADRHHDRSKRDRDRDSHANNDKDSRRVG 300

Query: 301 EPRYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADED 360
           EPRYYES+ASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQR+KRLKKA ED
Sbjct: 301 EPRYYESHASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAED 360

Query: 361 DALEAKQNAVPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR 418
           DALEAKQNAVPSSRNFLD AQKRMYGAEKGGSSTIEESIRRRTYYSQGKS+IEANAFRR
Sbjct: 361 DALEAKQNAVPSSRNFLDTAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 419

BLAST of CSPI04G04220 vs. ExPASy TrEMBL
Match: A0A6J1CHZ1 (pre-mRNA-splicing factor CWC25 homolog OS=Momordica charantia OX=3673 GN=LOC111011671 PE=3 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 7.2e-173
Identity = 353/417 (84.65%), Postives = 381/417 (91.37%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120
           LVPKQERLDFLYESGLAVGK SSSDGFKSLETLPS+S   A TEPSSSKEAA PGALFE+
Sbjct: 61  LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFEN 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180
           KPHSAND+WRKLHSDPLLIIRQREQ+ALARVKNNPIQMAMIRKTV+VEKHK+KNPDDKR+
Sbjct: 121 KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRK 180

Query: 181 RKKHRHSKSKRHKDSSPERDYDSEDVSPERQRSKHDHDKSSRHDGHSHSEDRRSKADTKN 240
           RKKH HSKSKRHKD S ERDYDSE+ SPERQR +HD DKSS+HD H +SEDRRSK D KN
Sbjct: 181 RKKHNHSKSKRHKDFSSERDYDSENASPERQR-RHDRDKSSKHD-HFYSEDRRSKTDFKN 240

Query: 241 ERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRHHDRSKRDRDSYANNDRDSRRVGEP 300
           ER  DRGSKYAA  S DQSDRKT K++ H S A+RHHDRSKR+RDSYA+++++SRRVGE 
Sbjct: 241 ERVHDRGSKYAAH-STDQSDRKTSKTSTHGS-AERHHDRSKRERDSYASSEKNSRRVGEL 300

Query: 301 RYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADEDDA 360
           R YESN+SETP+ESRH+HRR   KLSEEERAARLREMQQDAELH+EQR++RLKKA EDDA
Sbjct: 301 RSYESNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAEDDA 360

Query: 361 LEAKQNAVPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR 418
           LEAKQ A PS +NFLD AQKRMYGAEKGGSSTIEESIRRRTYYSQGKS+IEANAFRR
Sbjct: 361 LEAKQAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 413

BLAST of CSPI04G04220 vs. ExPASy TrEMBL
Match: A0A6J1E963 (LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog OS=Cucurbita moschata OX=3662 GN=LOC111430542 PE=3 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.6e-170
Identity = 355/422 (84.12%), Postives = 371/422 (87.91%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLP-SSSTAAAATEPSSSKEAAVPGALFE 120
           LVPKQERLDFLYESGLAVGK S+SDGFKSLETLP ++STAAAA EPSSSKEAA PGALFE
Sbjct: 61  LVPKQERLDFLYESGLAVGKGSNSDGFKSLETLPTTTSTAAAAPEPSSSKEAAAPGALFE 120

Query: 121 DKPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKR 180
           DKPHSAND WRKLHSDPLLIIRQREQ ALARVKNNPIQMAMIRKTVEVEKHK KNPDD+ 
Sbjct: 121 DKPHSANDAWRKLHSDPLLIIRQREQXALARVKNNPIQMAMIRKTVEVEKHKAKNPDDEG 180

Query: 181 ERKKHRHSKSKRHKDSSPERDYDSEDVSPERQRSK---HDHDKSSRHDGHSHSEDRRSKA 240
           ERKKH HSKSKR KDS P R+YDSE  SPERQR K   HD DKSS+ D  S+SEDRRSK 
Sbjct: 181 ERKKHCHSKSKRRKDSPPGREYDSESASPERQRRKRRNHDRDKSSKRDVRSYSEDRRSKT 240

Query: 241 DTKNERDRDRGSKYAARTSYDQSDRKTFKSNPHD-SAADRHHDRSKRDRDSYANNDRDSR 300
           D  NERDRD GSK      YDQSDRKT KSNPHD  A +RHHD SKR RDSY +N++DSR
Sbjct: 241 DYMNERDRDSGSK------YDQSDRKTPKSNPHDYYAEERHHDSSKRYRDSYTHNEKDSR 300

Query: 301 RVGEPRYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKA 360
           RVGE R YE NAS TP E RH+HRRP TKLSEEERAARLREMQQDAE HEEQR++RLKKA
Sbjct: 301 RVGESRTYEPNASNTPFEPRHKHRRPATKLSEEERAARLREMQQDAEFHEEQRWRRLKKA 360

Query: 361 DEDDALEAKQNAVPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSQIEANAF 418
            EDDALEA+QNAVPSSRNFLD AQKRMY AEKGGSSTIEESIRRRTYYSQGKS+IEANAF
Sbjct: 361 AEDDALEAEQNAVPSSRNFLDTAQKRMYSAEKGGSSTIEESIRRRTYYSQGKSEIEANAF 416

BLAST of CSPI04G04220 vs. NCBI nr
Match: XP_004146897.1 (pre-mRNA-splicing factor CWC25 homolog [Cucumis sativus] >KGN53175.1 hypothetical protein Csa_014942 [Cucumis sativus])

HSP 1 Score: 748.0 bits (1930), Expect = 4.3e-212
Identity = 411/417 (98.56%), Postives = 413/417 (99.04%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120
           LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180
           KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE
Sbjct: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180

Query: 181 RKKHRHSKSKRHKDSSPERDYDSEDVSPERQRSKHDHDKSSRHDGHSHSEDRRSKADTKN 240
           RKKHRHSKSKRHKDSSPERDYDSEDVSPERQR KHDHDKSSRHDGHSHSEDRRSKA+TKN
Sbjct: 181 RKKHRHSKSKRHKDSSPERDYDSEDVSPERQRRKHDHDKSSRHDGHSHSEDRRSKAETKN 240

Query: 241 ERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRHHDRSKRDRDSYANNDRDSRRVGEP 300
           ERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADR+HDRSKRDRDSYA NDRDSRRVGE 
Sbjct: 241 ERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRYHDRSKRDRDSYATNDRDSRRVGEL 300

Query: 301 RYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADEDDA 360
           RYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADEDDA
Sbjct: 301 RYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADEDDA 360

Query: 361 LEAKQNAVPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR 418
           LEAKQNAVPSSRNFLDMAQKRMY AEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR
Sbjct: 361 LEAKQNAVPSSRNFLDMAQKRMYSAEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR 417

BLAST of CSPI04G04220 vs. NCBI nr
Match: XP_008453851.1 (PREDICTED: pre-mRNA-splicing factor CWC25 homolog [Cucumis melo] >KAA0044681.1 pre-mRNA-splicing factor CWC25-like protein [Cucumis melo var. makuwa] >TYK16902.1 pre-mRNA-splicing factor CWC25-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 729.2 bits (1881), Expect = 2.0e-206
Identity = 403/419 (96.18%), Postives = 409/419 (97.61%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120
           LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAA PGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAAPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180
           KPHSAND WRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE
Sbjct: 121 KPHSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180

Query: 181 RKKHRHSKSKRHKDSSPERDYDSEDVSPERQRSKHDHDKSSRHDGHSHSEDRRSKADTKN 240
           RKKHRHSKSKRHKDSSPERDYDSED SPERQR KHDHDKSSRHDGHSHSEDRRSKAD+KN
Sbjct: 181 RKKHRHSKSKRHKDSSPERDYDSEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 240

Query: 241 ERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRHHDRSK--RDRDSYANNDRDSRRVG 300
           ERDRDRGSKYAARTSYDQSDRKT KSNP DSAADRHHDRSK  RDRDS+ANND+DSRRVG
Sbjct: 241 ERDRDRGSKYAARTSYDQSDRKTSKSNPRDSAADRHHDRSKRDRDRDSHANNDKDSRRVG 300

Query: 301 EPRYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADED 360
           EPRYYES+ASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQR+KRLKKA ED
Sbjct: 301 EPRYYESHASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAED 360

Query: 361 DALEAKQNAVPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR 418
           DALEAKQNAVPSSRNFLD AQKRMYGAEKGGSSTIEESIRRRTYYSQGKS+IEANAFRR
Sbjct: 361 DALEAKQNAVPSSRNFLDTAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 419

BLAST of CSPI04G04220 vs. NCBI nr
Match: XP_038896474.1 (pre-mRNA-splicing factor CWC25 homolog [Benincasa hispida])

HSP 1 Score: 690.6 bits (1781), Expect = 8.1e-195
Identity = 382/417 (91.61%), Postives = 398/417 (95.44%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120
           LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180
           KPHSAND WRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHK+KNPD KRE
Sbjct: 121 KPHSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKEKNPDGKRE 180

Query: 181 RKKHRHSKSKRHKDSSPERDYDSEDVSPERQRSKHDHDKSSRHDGHSHSEDRRSKADTKN 240
           RKKHRHSKSKRHKDSS ERDYDSED SPERQR KHDH+ SS+HD HSH EDRRSKAD+KN
Sbjct: 181 RKKHRHSKSKRHKDSSSERDYDSEDASPERQRRKHDHENSSKHDAHSHLEDRRSKADSKN 240

Query: 241 ERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRHHDRSKRDRDSYANNDRDSRRVGEP 300
           ERDRDRGSKYAART+YDQSDR+T KSNPHDSAA+RHHDRS+RDRDSYANN+++SRRVGEP
Sbjct: 241 ERDRDRGSKYAARTTYDQSDRRTSKSNPHDSAAERHHDRSRRDRDSYANNEKNSRRVGEP 300

Query: 301 RYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADEDDA 360
           R Y SNASET +ESRH+HRRP TKLSEEER ARL EMQQDAELHEEQR++RLKKA EDDA
Sbjct: 301 RSYGSNASET-HESRHKHRRPATKLSEEERVARLHEMQQDAELHEEQRWRRLKKAAEDDA 360

Query: 361 LEAKQNAVPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR 418
           LEAKQNA PSSRNFLD AQKRMYGAEKGGSSTIEESIRRRTYYSQGKS+IEANAFRR
Sbjct: 361 LEAKQNAAPSSRNFLDTAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 416

BLAST of CSPI04G04220 vs. NCBI nr
Match: XP_023551879.1 (LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 622.9 bits (1605), Expect = 2.1e-174
Identity = 358/418 (85.65%), Postives = 376/418 (89.95%), Query Frame = 0

Query: 2   ALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAGL 61
           ALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAGL
Sbjct: 7   ALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFRLLQEQAGL 66

Query: 62  VPKQERLDFLYESGLAVGKASSSDGFKSLETLP-SSSTAAAATEPSSSKEAAVPGALFED 121
           VPKQERLDFLYESGLAVGK S+SDGFKSLETLP ++STAAAATEPSSSKEAA PGALFED
Sbjct: 67  VPKQERLDFLYESGLAVGKGSNSDGFKSLETLPTTTSTAAAATEPSSSKEAAAPGALFED 126

Query: 122 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 181
           KPHSAND+WRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE
Sbjct: 127 KPHSANDSWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 186

Query: 182 RKKHRHSKSKRHKDSSPERDYDSEDVSPERQRSKHDHDKSSRHDGHSHSEDRRSKADTKN 241
           RKKHRHSKSKR K SSPERDYDSE VSPE QR  HD DKSS+HD HS+SEDRRSK D  N
Sbjct: 187 RKKHRHSKSKRRKASSPERDYDSESVSPEMQRRNHDRDKSSKHDVHSYSEDRRSKTDYVN 246

Query: 242 ERDRDRGSKYAARTSYDQSDRKTFKSNPHD-SAADRHHDRSKRDRDSYANNDRDSRRVGE 301
           ERDRDRGSK      YDQSDRKT KSNPHD  A +RHHD  KRDRDSY +N++DS +VGE
Sbjct: 247 ERDRDRGSK------YDQSDRKTPKSNPHDYYAEERHHDSXKRDRDSYTHNEKDSTKVGE 306

Query: 302 PRYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADEDD 361
            R  E NAS++P E RH+HRRP TKLSEE RAARLREMQQDAE HEEQR++RLKKA EDD
Sbjct: 307 SRTREPNASKSPYEPRHKHRRPATKLSEEGRAARLREMQQDAEFHEEQRWRRLKKAAEDD 366

Query: 362 ALEAKQNAVPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR 418
           ALEA+QNAVPSSRNFLD AQKRMYGAEKGGSSTIEESI  RTYYSQGKS+IEANAFRR
Sbjct: 367 ALEAEQNAVPSSRNFLDTAQKRMYGAEKGGSSTIEESICWRTYYSQGKSEIEANAFRR 418

BLAST of CSPI04G04220 vs. NCBI nr
Match: XP_022141219.1 (pre-mRNA-splicing factor CWC25 homolog [Momordica charantia])

HSP 1 Score: 616.7 bits (1589), Expect = 1.5e-172
Identity = 353/417 (84.65%), Postives = 381/417 (91.37%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120
           LVPKQERLDFLYESGLAVGK SSSDGFKSLETLPS+S   A TEPSSSKEAA PGALFE+
Sbjct: 61  LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFEN 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180
           KPHSAND+WRKLHSDPLLIIRQREQ+ALARVKNNPIQMAMIRKTV+VEKHK+KNPDDKR+
Sbjct: 121 KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRK 180

Query: 181 RKKHRHSKSKRHKDSSPERDYDSEDVSPERQRSKHDHDKSSRHDGHSHSEDRRSKADTKN 240
           RKKH HSKSKRHKD S ERDYDSE+ SPERQR +HD DKSS+HD H +SEDRRSK D KN
Sbjct: 181 RKKHNHSKSKRHKDFSSERDYDSENASPERQR-RHDRDKSSKHD-HFYSEDRRSKTDFKN 240

Query: 241 ERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRHHDRSKRDRDSYANNDRDSRRVGEP 300
           ER  DRGSKYAA  S DQSDRKT K++ H S A+RHHDRSKR+RDSYA+++++SRRVGE 
Sbjct: 241 ERVHDRGSKYAAH-STDQSDRKTSKTSTHGS-AERHHDRSKRERDSYASSEKNSRRVGEL 300

Query: 301 RYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADEDDA 360
           R YESN+SETP+ESRH+HRR   KLSEEERAARLREMQQDAELH+EQR++RLKKA EDDA
Sbjct: 301 RSYESNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAEDDA 360

Query: 361 LEAKQNAVPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR 418
           LEAKQ A PS +NFLD AQKRMYGAEKGGSSTIEESIRRRTYYSQGKS+IEANAFRR
Sbjct: 361 LEAKQAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 413

BLAST of CSPI04G04220 vs. TAIR 10
Match: AT2G44200.1 (CBF1-interacting co-repressor CIR, N-terminal;Pre-mRNA splicing factor )

HSP 1 Score: 321.6 bits (823), Expect = 9.2e-88
Identity = 233/494 (47.17%), Postives = 293/494 (59.31%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           M +KFLNKKGWHTGSLRNIE VWKAEQK EAEQKKL+ELR QIL+E+ER EFR LQEQAG
Sbjct: 1   MGMKFLNKKGWHTGSLRNIETVWKAEQKQEAEQKKLEELRLQILQERERSEFRALQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSK-EAAVPGALFE 120
           L+P+QERL+FLY+SGLAVGK S+S    S +        + A   +S K + + PGALFE
Sbjct: 61  LIPRQERLEFLYDSGLAVGKGSASGSGVSFQKEEQPLAKSEAGSSASEKPDQSAPGALFE 120

Query: 121 DKPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKR 180
           +K  SAND+WRKLHSDPLL+IRQREQ+ALA++KNNP++MA+IRK+VE EK K K+ D K 
Sbjct: 121 EKAQSANDSWRKLHSDPLLLIRQREQEALAKIKNNPVKMALIRKSVE-EKGKGKDGDTKE 180

Query: 181 ERKKHRHSKSKRHKDSSPERDYDSEDVSPERQ--RSKHDHDKSSRHDGH----------- 240
            +KKH+    K  K SS  +  DSE+ S E    R  H    S  HD H           
Sbjct: 181 HKKKHKRKSGKHQKQSSSRQRSDSEEDSGEENNGRKSHHQKTSGTHDRHYERPRSDLEDE 240

Query: 241 --------SHSEDRRSKADTKNERDRDRGSKYAARTSY--DQSDRKTFKSNPHD------ 300
                    H E RRS+ D  ++R     + Y  R S   D+S R+  + N ++      
Sbjct: 241 SKGRESRDRHYEKRRSELDDGHKRRERHDTHYERRRSEMDDESKRRESRDNHYERRRSDL 300

Query: 301 -------SAADRHHDRSKRDRDSYAN-------------NDRDSRRVGEP---------- 360
                   + D+H +R + D D                 +D   RR   P          
Sbjct: 301 DDESKRRESHDKHFERQRSDLDDEYKRRESQDKRRRSDIDDEPKRRDARPNEKYRNRSPK 360

Query: 361 -----------------RYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAEL 418
                            R  E   S  PNE ++R R+  +KLSEEERAARL++MQ DAE+
Sbjct: 361 GGVERENLKSYGQEDKKRKAEDLDSGKPNEYQNRRRKGGSKLSEEERAARLKQMQMDAEV 420

BLAST of CSPI04G04220 vs. TAIR 10
Match: AT2G44195.1 (CBF1-interacting co-repressor CIR, N-terminal;Pre-mRNA splicing factor )

HSP 1 Score: 143.7 bits (361), Expect = 3.4e-34
Identity = 83/149 (55.70%), Postives = 101/149 (67.79%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGS+RN+E VWKAEQKHEAEQ+K++          ER EFR +QEQAG
Sbjct: 1   MALKFLNKKGWHTGSIRNVEKVWKAEQKHEAEQEKIE----------ERSEFRAIQEQAG 60

Query: 61  LVPKQ-ERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALF- 120
           LVP++ ERL+FLY+  LAV K          + + SS              A +PGALF 
Sbjct: 61  LVPRRPERLEFLYDPELAVAK----------QNVSSSRHGVLFQNQDQRARATIPGALFD 120

Query: 121 -EDKPHSANDTWRKLHSDPLLIIRQREQQ 147
            +DK HSAND+WRK HSDPLL+IRQ+EQ+
Sbjct: 121 DDDKRHSANDSWRKFHSDPLLLIRQKEQE 129

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9NXE83.7e-1729.25Pre-mRNA-splicing factor CWC25 homolog OS=Homo sapiens OX=9606 GN=CWC25 PE=1 SV=... [more]
Q9DBF73.7e-1728.90Pre-mRNA-splicing factor CWC25 homolog OS=Mus musculus OX=10090 GN=Cwc25 PE=2 SV... [more]
Q5B0I11.4e-1129.70Pre-mRNA-splicing factor cwc25 OS=Emericella nidulans (strain FGSC A4 / ATCC 381... [more]
Q4P6I34.4e-1027.36Pre-mRNA-splicing factor CWC25 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=23... [more]
Q4X1D71.3e-0927.58Pre-mRNA-splicing factor cwc25 OS=Neosartorya fumigata (strain ATCC MYA-4609 / A... [more]
Match NameE-valueIdentityDescription
A0A0A0KU622.1e-21298.56Cir_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G025080 PE=3 ... [more]
A0A5A7TSZ09.9e-20796.18Pre-mRNA-splicing factor CWC25-like protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BY159.9e-20796.18pre-mRNA-splicing factor CWC25 homolog OS=Cucumis melo OX=3656 GN=LOC103494454 P... [more]
A0A6J1CHZ17.2e-17384.65pre-mRNA-splicing factor CWC25 homolog OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1E9632.6e-17084.12LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog OS=Cucurbita moschat... [more]
Match NameE-valueIdentityDescription
XP_004146897.14.3e-21298.56pre-mRNA-splicing factor CWC25 homolog [Cucumis sativus] >KGN53175.1 hypothetica... [more]
XP_008453851.12.0e-20696.18PREDICTED: pre-mRNA-splicing factor CWC25 homolog [Cucumis melo] >KAA0044681.1 p... [more]
XP_038896474.18.1e-19591.61pre-mRNA-splicing factor CWC25 homolog [Benincasa hispida][more]
XP_023551879.12.1e-17485.65LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog, partial [Cucurbita ... [more]
XP_022141219.11.5e-17284.65pre-mRNA-splicing factor CWC25 homolog [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT2G44200.19.2e-8847.17CBF1-interacting co-repressor CIR, N-terminal;Pre-mRNA splicing factor [more]
AT2G44195.13.4e-3455.70CBF1-interacting co-repressor CIR, N-terminal;Pre-mRNA splicing factor [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 22..49
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 150..417
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 163..179
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 310..363
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 193..302
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 395..411
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 85..105
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 80..129
NoneNo IPR availablePANTHERPTHR16196CELL CYCLE CONTROL PROTEIN CWF25coord: 1..417
IPR019339CBF1-interacting co-repressor CIR, N-terminal domainSMARTSM01083Cir_N_3coord: 10..46
e-value: 2.4E-13
score: 60.3
IPR019339CBF1-interacting co-repressor CIR, N-terminal domainPFAMPF10197Cir_Ncoord: 11..46
e-value: 9.8E-15
score: 54.5
IPR022209Pre-mRNA splicing factorPFAMPF12542CWC25coord: 64..155
e-value: 5.3E-12
score: 46.5

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G04220.1CSPI04G04220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
cellular_component GO:0005684 U2-type spliceosomal complex