CSPI04G03660 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G03660
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionsalutaridinol 7-O-acetyltransferase-like
LocationChr4: 2227393 .. 2229128 (+)
RNA-Seq ExpressionCSPI04G03660
SyntenyCSPI04G03660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGTGTTTGTTTCATGATAAGATTATACAAAAAAAAATGGAGGTTAAAGTTTTGTCAAAAGAAACTATTATACCTTCTTCCCCAACTCCCCCTCACCTTCAAACCTTTCAACTTTCACTACTTGATCAGTTGTCTCCAATGCTTTATATTCCACTTTTACTTTTCTACCCCATGAAAAGAAGTTATGATCATATTGATCAACATGACCAAGATCCTAAGAAAACAATTGCTACCCTAAAAACTTCTTTATCAAAAACACTGAGTCGTTTTTACTTATTGGCAGGAAGAATCATTGACAAATCTATCCATTGTAATGACAAAGGAGCTGTGTTTATGGAAGCAACCATAAACACCAACATGTTTGACATCCTCAAAGAACCAAACAATGAGGTTTTAACAAAACTCCTTCCATGTTCTTTGTTGTGTAACACTAAACCAATTGAAGAATATCCTCAAATTGTTGTTCAAGCCAACGTTTTCAAATGTGGTGGTATTGCAATCTCTCTCTGTCTATTGCACAAACTTATTGATGCAGCTACTTTCTGCTGTTTTCTAAGATCATGGGCCACTACCAATAGAGAATTGTTATCTCAGCTTGATCATTCATCTCCAAATAATATGGTATGTTTTGACTATAAATCATTCTCTTCCCTCTTTCCGCAAACCAATTTGTTGCCTTTTCATCAAAGACCGATCAATAATGATAACGCGGTCATACTGCCGTCGAGTATTTTCAACGGTAAACGTCGTTTACAAAGATTCGTGTTCCGATCGAAAGCGATATTAGACCTTAAAGCTAAAGCGAAATCCTGTGATATACCAAATCCAACATGTGTTGAAGCAATAACATGCTTTATTTGGAAATATCTCATGAAAGTTGCTGATGGTGGTGATTCTCAAATGCCATCAACTCTGTCTCATGTTGTAAACATCAGAAAGATGATAGAACCATCACTGGGTGAAGTTTGTTTGGGGAACATTATGTGGGGCACAGTTGCTCATCATTTTTCAACCACAAGGCCGAATGAAGCATTCGAGGGTTTGGAGCTAAGCAAATTGGTAAGTTTATTGAGACAATCATTGAAAAAGATCAATAAAGATTATATAAAAGAGTTGATTATGGGAGGTGATAAAGGAAGAAGAAATGGTGTTATGAAGTTGGTTGGTGAGATAAATAAGTGGCCAATCTCAAATTATTACTTCTTCACAAGTTGGAAAAATATGAAGTTGAATGAAGTTGACTTTGGGTGGGGAAAACCTTTATGGTTTGGAATTGCTGGGGACTCGAATGAAATGATGGGAAACATTATTGTTTTGGTAGATAATGTATCGGATGATGGATCAATAGAGGCATGGATTCTCTTAGATGAAAAAGAAATGCAACTCTTAGAACAAAATCCACAATTTCTTGAGTTTGCATTGCTGAATCCAAGTATACATTTGCCCCATGATCACAAAATTGCTGATCAGATTTTCTCTAGAAAATTAATATGATGTCTCCAGTGTGATGATGATGGTGTCGACTTAGTGGAAATAACCTATGTGTCTACTGATATTGCTCGACACTTGTAGACGTATTTTATTAAGAAAATGTGATTTCTATGTTAGTTTTTCAAGTATATAGTTGATCTTTCTATTATTATTAATGTTTAGAAAATGATCCATAAGATGTAATAATTAAATAGTAAGGTTTAATTTCAATTTCGACTAAATAAATGGATATGTTTCGAGAGGGT

mRNA sequence

TAGTGTTTGTTTCATGATAAGATTATACAAAAAAAAATGGAGGTTAAAGTTTTGTCAAAAGAAACTATTATACCTTCTTCCCCAACTCCCCCTCACCTTCAAACCTTTCAACTTTCACTACTTGATCAGTTGTCTCCAATGCTTTATATTCCACTTTTACTTTTCTACCCCATGAAAAGAAGTTATGATCATATTGATCAACATGACCAAGATCCTAAGAAAACAATTGCTACCCTAAAAACTTCTTTATCAAAAACACTGAGTCGTTTTTACTTATTGGCAGGAAGAATCATTGACAAATCTATCCATTGTAATGACAAAGGAGCTGTGTTTATGGAAGCAACCATAAACACCAACATGTTTGACATCCTCAAAGAACCAAACAATGAGGTTTTAACAAAACTCCTTCCATGTTCTTTGTTGTGTAACACTAAACCAATTGAAGAATATCCTCAAATTGTTGTTCAAGCCAACGTTTTCAAATGTGGTGGTATTGCAATCTCTCTCTGTCTATTGCACAAACTTATTGATGCAGCTACTTTCTGCTGTTTTCTAAGATCATGGGCCACTACCAATAGAGAATTGTTATCTCAGCTTGATCATTCATCTCCAAATAATATGGTATGTTTTGACTATAAATCATTCTCTTCCCTCTTTCCGCAAACCAATTTGTTGCCTTTTCATCAAAGACCGATCAATAATGATAACGCGGTCATACTGCCGTCGAGTATTTTCAACGGTAAACGTCGTTTACAAAGATTCGTGTTCCGATCGAAAGCGATATTAGACCTTAAAGCTAAAGCGAAATCCTGTGATATACCAAATCCAACATGTGTTGAAGCAATAACATGCTTTATTTGGAAATATCTCATGAAAGTTGCTGATGGTGGTGATTCTCAAATGCCATCAACTCTGTCTCATGTTGTAAACATCAGAAAGATGATAGAACCATCACTGGGTGAAGTTTGTTTGGGGAACATTATGTGGGGCACAGTTGCTCATCATTTTTCAACCACAAGGCCGAATGAAGCATTCGAGGGTTTGGAGCTAAGCAAATTGGTAAGTTTATTGAGACAATCATTGAAAAAGATCAATAAAGATTATATAAAAGAGTTGATTATGGGAGGTGATAAAGGAAGAAGAAATGGTGTTATGAAGTTGGTTGGTGAGATAAATAAGTGGCCAATCTCAAATTATTACTTCTTCACAAGTTGGAAAAATATGAAGTTGAATGAAGTTGACTTTGGGTGGGGAAAACCTTTATGGTTTGGAATTGCTGGGGACTCGAATGAAATGATGGGAAACATTATTGTTTTGGTAGATAATGTATCGGATGATGGATCAATAGAGGCATGGATTCTCTTAGATGAAAAAGAAATGCAACTCTTAGAACAAAATCCACAATTTCTTGAGTTTGCATTGCTGAATCCAAGTATACATTTGCCCCATGATCACAAAATTGCTGATCAGATTTTCTCTAGAAAATTAATATGATGTCTCCAGTGTGATGATGATGGTGTCGACTTAGTGGAAATAACCTATGTGTCTACTGATATTGCTCGACACTTGTAGACGTATTTTATTAAGAAAATGTGATTTCTATGTTAGTTTTTCAAGTATATAGTTGATCTTTCTATTATTATTAATGTTTAGAAAATGATCCATAAGATGTAATAATTAAATAGTAAGGTTTAATTTCAATTTCGACTAAATAAATGGATATGTTTCGAGAGGGT

Coding sequence (CDS)

ATGGAGGTTAAAGTTTTGTCAAAAGAAACTATTATACCTTCTTCCCCAACTCCCCCTCACCTTCAAACCTTTCAACTTTCACTACTTGATCAGTTGTCTCCAATGCTTTATATTCCACTTTTACTTTTCTACCCCATGAAAAGAAGTTATGATCATATTGATCAACATGACCAAGATCCTAAGAAAACAATTGCTACCCTAAAAACTTCTTTATCAAAAACACTGAGTCGTTTTTACTTATTGGCAGGAAGAATCATTGACAAATCTATCCATTGTAATGACAAAGGAGCTGTGTTTATGGAAGCAACCATAAACACCAACATGTTTGACATCCTCAAAGAACCAAACAATGAGGTTTTAACAAAACTCCTTCCATGTTCTTTGTTGTGTAACACTAAACCAATTGAAGAATATCCTCAAATTGTTGTTCAAGCCAACGTTTTCAAATGTGGTGGTATTGCAATCTCTCTCTGTCTATTGCACAAACTTATTGATGCAGCTACTTTCTGCTGTTTTCTAAGATCATGGGCCACTACCAATAGAGAATTGTTATCTCAGCTTGATCATTCATCTCCAAATAATATGGTATGTTTTGACTATAAATCATTCTCTTCCCTCTTTCCGCAAACCAATTTGTTGCCTTTTCATCAAAGACCGATCAATAATGATAACGCGGTCATACTGCCGTCGAGTATTTTCAACGGTAAACGTCGTTTACAAAGATTCGTGTTCCGATCGAAAGCGATATTAGACCTTAAAGCTAAAGCGAAATCCTGTGATATACCAAATCCAACATGTGTTGAAGCAATAACATGCTTTATTTGGAAATATCTCATGAAAGTTGCTGATGGTGGTGATTCTCAAATGCCATCAACTCTGTCTCATGTTGTAAACATCAGAAAGATGATAGAACCATCACTGGGTGAAGTTTGTTTGGGGAACATTATGTGGGGCACAGTTGCTCATCATTTTTCAACCACAAGGCCGAATGAAGCATTCGAGGGTTTGGAGCTAAGCAAATTGGTAAGTTTATTGAGACAATCATTGAAAAAGATCAATAAAGATTATATAAAAGAGTTGATTATGGGAGGTGATAAAGGAAGAAGAAATGGTGTTATGAAGTTGGTTGGTGAGATAAATAAGTGGCCAATCTCAAATTATTACTTCTTCACAAGTTGGAAAAATATGAAGTTGAATGAAGTTGACTTTGGGTGGGGAAAACCTTTATGGTTTGGAATTGCTGGGGACTCGAATGAAATGATGGGAAACATTATTGTTTTGGTAGATAATGTATCGGATGATGGATCAATAGAGGCATGGATTCTCTTAGATGAAAAAGAAATGCAACTCTTAGAACAAAATCCACAATTTCTTGAGTTTGCATTGCTGAATCCAAGTATACATTTGCCCCATGATCACAAAATTGCTGATCAGATTTTCTCTAGAAAATTAATATGA

Protein sequence

MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDPKKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVLTKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTNRELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRRLQRFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHVVNIRKMIEPSLGEVCLGNIMWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLKKINKDYIKELIMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNEVDFGWGKPLWFGIAGDSNEMMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSIHLPHDHKIADQIFSRKLI*
Homology
BLAST of CSPI04G03660 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.2e-48
Identity = 150/479 (31.32%), Postives = 230/479 (48.02%), Query Frame = 0

Query: 3   VKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPM-LYIPLLLFYP--MKRSYDHIDQHDQD 62
           V+V+SKETI P++PTP  L+ F LSLLDQ  P+  Y+P++LFYP     S    + HD  
Sbjct: 9   VEVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSNHHDD- 68

Query: 63  PKKTIATLKTSLSKTLSRFYLLAGRIIDK-SIHCNDKGAVFMEATINTNMFDILKEPNNE 122
               +  LK+SLSKTL  FY +AGR+ID   + C+D+G  F +  I   M + + +P + 
Sbjct: 69  ----LDLLKSSLSKTLVHFYPMAGRMIDNILVDCHDQGINFYKVKIRGKMCEFMSQP-DV 128

Query: 123 VLTKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWAT 182
            L++LLP  ++  + P E    ++VQ N+F CGG AI   + HK+ DAAT   F+RSWA+
Sbjct: 129 PLSQLLPSEVVSASVPKEAL--VIVQVNMFDCGGTAICSSVSHKIADAATMSTFIRSWAS 188

Query: 183 TNRELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLP----FHQRPINNDNAVILPSSIFN 242
           T +   S    ++  +         +SLFP +  L       + P ++      P    +
Sbjct: 189 TTKTSRSGGSTAAVTDQKLIPSFDSASLFPPSERLTSPSGMSEIPFSS-----TPEDTED 248

Query: 243 GKRRLQRFVFRSKAILDLKAKAKSCDIPN-----PTCVEAITCFIWKYLMKVADGGDSQM 302
            K   +RFVF    I  ++ K +     N      T VE +T  IWK +MK    G    
Sbjct: 249 DKTVSKRFVFDFAKITSVREKLQVLMHDNYKSRRQTRVEVVTSLIWKSVMKSTPAG---F 308

Query: 303 PSTLSHVVNIRKMIEPSLGEVCLGNIMWGTVAHHFSTTRP-----NEAFEGL-------- 362
              + H VN+RK ++P L +V  GN+     A   +TT       N+             
Sbjct: 309 LPVVHHAVNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTTTNAVNKTINSTSSESQVVL 368

Query: 363 -ELSKLVSLLRQSLKKI--NKDYIKELIMGGDKGRRNGVMKLVGEINKWPISNYYFFTSW 422
            EL   ++ +R  + K+  +K  ++++I     G    + K    IN   + N++  +SW
Sbjct: 369 HELHDFIAQMRSEIDKVKGDKGSLEKVIQNFASGHDASIKK----INDVEVINFW-ISSW 428

Query: 423 KNMKLNEVDFGWGKPLWFGIAGDSNEMMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLE 453
             M L E+DFGWGKP+W  +  D N         +++      IE W    E +M   E
Sbjct: 429 CRMGLYEIDFGWGKPIW--VTVDPNIKPNKNCFFMNDTKCGEGIEVWASFLEDDMAKFE 464

BLAST of CSPI04G03660 vs. ExPASy Swiss-Prot
Match: O23393 (BAHD acyltransferase BIA1 OS=Arabidopsis thaliana OX=3702 GN=BIA1 PE=2 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 6.2e-48
Identity = 149/473 (31.50%), Postives = 246/473 (52.01%), Query Frame = 0

Query: 2   EVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDPK 61
           +++V  KE I P+SP+P      QLS+LD   P +Y+  + FY +      +   +    
Sbjct: 4   KLEVTGKEVIKPASPSP--RDRLQLSILDLYCPGIYVSTIFFYDLITESSEVFSEN---- 63

Query: 62  KTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVLT 121
                LK SLS+TLSRFY LAGRI   SI CND+GAVF EA  +  + D L+  N + L+
Sbjct: 64  -----LKLSLSETLSRFYPLAGRIEGLSISCNDEGAVFTEARTDLLLPDFLRNLNTDSLS 123

Query: 122 KLLPCSLLCNTKPIEEYPQIVVQANVFKCG-GIAISLCLLHKLIDAATFCCFLRSWATTN 181
             LP +L     P   +P + V+   F  G G+A+S+ + HK+ D A+   F++ WATT 
Sbjct: 124 GFLP-TLAAGESP-AAWPLLSVKVTFFGSGSGVAVSVSVSHKICDIASLVTFVKDWATTT 183

Query: 182 RELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRRLQ 241
            +          N+ + F   + ++++P      + Q P  + ++ I        K  L+
Sbjct: 184 AK-------GKSNSTIEF---AETTIYPPPPSHMYEQFPSTDSDSNI------TSKYVLK 243

Query: 242 RFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMP--STLSHVVN 301
           RFVF    I +LK KA S  +P PT VEAI   IW+   + +   +  +P  + +   ++
Sbjct: 244 RFVFEPSKIAELKHKAASESVPVPTRVEAIMSLIWR-CARNSSRSNLLIPRQAVMWQAMD 303

Query: 302 IRKMIEPSLG-EVCLGNIMWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLKKINKDYI 361
           IR  I  S+  +  +GN+  G     FS  +  +A    E+ ++V+  R++ +++N + I
Sbjct: 304 IRLRIPSSVAPKDVIGNLQSG-----FSLKK--DAESEFEIPEIVATFRKNKERVN-EMI 363

Query: 362 KELIMGGDKGRRNGVMKLVGE-INKWPISNYYFFTSWKNMKLNEVDFGWGKPLWFGIAGD 421
           KE + G   G+   ++ L+ E +++    + Y  +SW      EVDFG G P+W G A  
Sbjct: 364 KESLQGNTIGQ--SLLSLMAETVSESTEIDRYIMSSWCRKPFYEVDFGSGSPVWVGYASH 423

Query: 422 S--NEMMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSI 468
           +  + M+G  +VL+D+   DG +EAWI L E++M +   + + L +A+LNP +
Sbjct: 424 TIYDNMVG--VVLIDSKEGDG-VEAWISLPEEDMSVFVDDQELLAYAVLNPPV 433

BLAST of CSPI04G03660 vs. ExPASy Swiss-Prot
Match: I3PLR4 ((13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver somniferum OX=3469 GN=AT1 PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 5.2e-47
Identity = 153/475 (32.21%), Postives = 226/475 (47.58%), Query Frame = 0

Query: 3   VKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDPKK 62
           V+V+SKETI P +PTP  L+ + +SLLDQ S ++Y+P++LFYP     +       D   
Sbjct: 9   VEVISKETIKPRNPTPYQLRNYNMSLLDQYSSLVYVPIILFYPAASDANSTGSKHHD--- 68

Query: 63  TIATLKTSLSKTLSRFYLLAGRIIDK-SIHCNDKGAVFMEATINTNMFDILKEPNNEVLT 122
            +  LK SLS+TL  FY +AGR+ D  ++ CND+G  F E  I   M D + + ++  L+
Sbjct: 69  DLHLLKRSLSETLVHFYPMAGRMKDNMTVDCNDEGIDFFEVRIKGRMCDFMMK-SDAHLS 128

Query: 123 KLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWA-TTN 182
            LLP S + +T  ++E  Q++VQ N+F CGG AI  C+ +K+ DA T   F+RS A TTN
Sbjct: 129 LLLP-SEVASTNFVKE-AQVIVQVNMFDCGGTAICFCISNKIADACTMITFIRSLAGTTN 188

Query: 183 RELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRRLQ 242
                    +   N         +SLFP +  L         D+  +        K   +
Sbjct: 189 IARRGSSIAAPTTNQNLVPSFDSTSLFPPSEQLASQVSYPTQDSTSV-------DKLVSK 248

Query: 243 RFVFRSKAILDLKAKAKSC-----DIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSH 302
           RFVF +  I   + K +S          PT VE ++  IWK  +K A  G     ST++H
Sbjct: 249 RFVFDAAKITSAREKLQSLMHDKYKCHRPTRVEVVSALIWKSAVKSAPPGSI---STVTH 308

Query: 303 VVNIRKMIEPSLGEVCLGNIMWGTV----AHHFSTTRP---------NEAFEGL-ELSKL 362
            +N RK ++P L +   GN+         A   +TT P         NE    L ELS  
Sbjct: 309 AMNFRKKMDPPLQDASFGNLCVVVTAVLPATTATTTNPATKKVSSTSNEEQVALDELSDF 368

Query: 363 VSLLRQSLKKI--NKDYIKELIMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLN 422
           V+LLR+ + K+  +K  ++++I     G    V K     +K       F TSW      
Sbjct: 369 VALLRREIDKVKGDKGCMEKIIQKFIYGHDASVAKDSDVEDK---VTALFMTSWCKFGFY 428

Query: 423 EVDFGWGKPLWFGIAGDSNEMMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQN 455
           E DFGWG P+W            N++ + D    +G IE W+   E +M   E +
Sbjct: 429 EADFGWGTPVWVTTVPLIEPKYKNMVFMNDMKCGEG-IEVWVNFLEDDMTKFEHH 463

BLAST of CSPI04G03660 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 1.2e-46
Identity = 144/459 (31.37%), Postives = 236/459 (51.42%), Query Frame = 0

Query: 2   EVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDPK 61
           ++++LS+E I PSSPTP  L+T +LS LDQ+    +IP++LFYP     + +D  + D  
Sbjct: 4   QMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYP-----NQLDS-NLDRA 63

Query: 62  KTIATLKTSLSKTLSRFYLLAGRI-IDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL 121
           +    LK SLS  L++FY LAGRI I+ S+ CND G  F+EA +++ + + +K    + L
Sbjct: 64  QRSENLKRSLSTVLTQFYPLAGRININSSVDCNDSGVPFLEARVHSQLSEAIKNVAIDEL 123

Query: 122 TKLLPCSLLCNTKP--IEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWAT 181
            + LP       +   +++   + V+ + F+CGG AI +C+ HK+ DA +   FL SW  
Sbjct: 124 NQYLPFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTA 183

Query: 182 TNRELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRR 241
           T +E   + D   PN    FD  S    FP    +P  +  + ++N V            
Sbjct: 184 TCQE---ETDIVQPN----FDLGSHH--FPPMESIPAPEF-LPDENIV------------ 243

Query: 242 LQRFVFRSKAILDLKAK--AKSCDIPNPTCVEAITCFIWKYLMKVADGG-DSQMPSTLSH 301
           ++RFVF  + +  LKA+  + + ++ N + V+ +   IWK  + V     D++     + 
Sbjct: 244 MKRFVFDKEKLEALKAQLASSATEVKNSSRVQIVIAVIWKQFIDVTRAKFDTKNKLVAAQ 303

Query: 302 VVNIRKMIEPSLGEVCLGNIMWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLKKINKD 361
            VN+R  + P   +  +GNI   T+A+         A E  + S LV  L+ SL KI+ +
Sbjct: 304 AVNLRSRMNPPFPQSAMGNI--ATMAYAV-------AEEDKDFSDLVGPLKTSLAKIDDE 363

Query: 362 YIKELIMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNEVDFGWGKPLWFGIAG 421
           ++KEL        + GV  L  E         + F+SW  +   ++DFGWGKP+      
Sbjct: 364 HVKEL--------QKGVTYLDYEAEP---QELFSFSSWCRLGFYDLDFGWGKPV---SVC 410

Query: 422 DSNEMMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQN 455
            +   M N++ L+D  ++DG +EAWI + E EM +L  +
Sbjct: 424 TTTVPMKNLVYLMDTRNEDG-MEAWISMAEDEMSMLSSD 410

BLAST of CSPI04G03660 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 174.9 bits (442), Expect = 2.3e-42
Identity = 138/458 (30.13%), Postives = 222/458 (48.47%), Query Frame = 0

Query: 2   EVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDPK 61
           +++ +S+E I+PSSPTP  L+ +++S LDQL    +IP +LFYP     + +D  + DP 
Sbjct: 4   QMEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYP-----NPLDS-NLDPA 63

Query: 62  KTIATLKTSLSKTLSRFYLLAGRI-IDKSIHCNDKGAVFMEATINTNMFDILKE-PNNEV 121
           +T   LK SLSK L+ FY LAGRI ++ S+ CND G  F+EA +   +   ++     E 
Sbjct: 64  QTSQHLKQSLSKVLTHFYPLAGRINVNSSVDCNDSGVPFVEARVQAQLSQAIQNVVELEK 123

Query: 122 LTKLLPCSLLCNTK-PIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWAT 181
           L + LP +     K  + E   + V+ + F+CGG AI + L HK+ D  +   FL +W  
Sbjct: 124 LDQYLPSAAYPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTA 183

Query: 182 TNRELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRR 241
           T R                        + P  +L   H  P++N  +   P  + +    
Sbjct: 184 TCR-------------------GETEIVLPNFDLAARHFPPVDNTPS---PELVPDENVV 243

Query: 242 LQRFVFRSKAILDLKAKAKSC-DIPNPTCVEAITCFIWKYLMKVADGG-DSQMPSTLSHV 301
           ++RFVF  + I  L+A+A S  +  N + V+ +  +IWK+++ V      ++    +   
Sbjct: 244 MKRFVFDKEKIGALRAQASSASEEKNFSRVQLVVAYIWKHVIDVTRAKYGAKNKFVVVQA 303

Query: 302 VNIRKMIEPSLGEVCLGNI---MWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLKKIN 361
           VN+R  + P L    +GNI   ++  V   +    P+          L+  LR SL+K  
Sbjct: 304 VNLRSRMNPPLPHYAMGNIATLLFAAVDAEWDKDFPD----------LIGPLRTSLEKTE 363

Query: 362 KDYIKELIMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNEVDFGWGKPLWFGI 421
            D+  EL+ G         M  + E+    + +   FTSW  +   ++DFGWGKPL    
Sbjct: 364 DDHNHELLKG---------MTCLYELEPQELLS---FTSWCRLGFYDLDFGWGKPL---S 407

Query: 422 AGDSNEMMGNIIVLVDNVSDDGSIEAWILLDEKEMQLL 452
           A  +     N  +L+D  S DG +EAW+ + E EM +L
Sbjct: 424 ACTTTFPKRNAALLMDTRSGDG-VEAWLPMAEDEMAML 407

BLAST of CSPI04G03660 vs. ExPASy TrEMBL
Match: A0A0A0KXH2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G016510 PE=3 SV=1)

HSP 1 Score: 983.0 bits (2540), Expect = 4.4e-283
Identity = 481/485 (99.18%), Postives = 483/485 (99.59%), Query Frame = 0

Query: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60
           MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP
Sbjct: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60

Query: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL 120
           KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL
Sbjct: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL 120

Query: 121 TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN 180
           TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN
Sbjct: 121 TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN 180

Query: 181 RELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRRLQ 240
           RELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQR INNDNAVILPSSIFNGKRRLQ
Sbjct: 181 RELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRLINNDNAVILPSSIFNGKRRLQ 240

Query: 241 RFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHVVNIR 300
           RFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHVVNIR
Sbjct: 241 RFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHVVNIR 300

Query: 301 KMIEPSLGEVCLGNIMWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLKKINKDYIKEL 360
           KMIEPSLGEV LGNIMWGTVAHHFSTTRPNE+FEGLELSKLVSLLRQSLKKINKDYIKEL
Sbjct: 301 KMIEPSLGEVSLGNIMWGTVAHHFSTTRPNESFEGLELSKLVSLLRQSLKKINKDYIKEL 360

Query: 361 IMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNEVDFGWGKPLWFGIAGDSNEM 420
           IMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNE+DFGWGKPLWFGIAGDSNEM
Sbjct: 361 IMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNELDFGWGKPLWFGIAGDSNEM 420

Query: 421 MGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSIHLPHDHKIADQIF 480
           MGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSIHLPHDHKIADQIF
Sbjct: 421 MGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSIHLPHDHKIADQIF 480

Query: 481 SRKLI 486
           SRKLI
Sbjct: 481 SRKLI 485

BLAST of CSPI04G03660 vs. ExPASy TrEMBL
Match: A0A1S3BXK4 (LOW QUALITY PROTEIN: salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103494516 PE=3 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 1.6e-248
Identity = 436/486 (89.71%), Postives = 447/486 (91.98%), Query Frame = 0

Query: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60
           MEVKVLSKETIIPSSPTPPHLQT QLSLLDQLSPMLYIPLLLFYPMK+SY H     QD 
Sbjct: 42  MEVKVLSKETIIPSSPTPPHLQTLQLSLLDQLSPMLYIPLLLFYPMKKSYQH-----QDH 101

Query: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL 120
            K IATLKTSLSKTLSRFYLLAGRII KSIHCNDKGAVFMEATIN+NMFDILKEPNNEVL
Sbjct: 102 NKAIATLKTSLSKTLSRFYLLAGRIIGKSIHCNDKGAVFMEATINSNMFDILKEPNNEVL 161

Query: 121 TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN 180
           TKLLPCSLLCNTKPIEEYPQIVVQAN+F+CGGIAISLCLLHKLIDAATFCCFLRSWATTN
Sbjct: 162 TKLLPCSLLCNTKPIEEYPQIVVQANIFECGGIAISLCLLHKLIDAATFCCFLRSWATTN 221

Query: 181 RELLSQLDHSSP-NNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRRL 240
           RE LS LDHSSP NNMVCFDYKSFSSLFPQTNLLPFHQ  INND  V+ PSSIFN KR  
Sbjct: 222 RESLS-LDHSSPNNNMVCFDYKSFSSLFPQTNLLPFHQSLINNDKXVVPPSSIFNRKRHF 281

Query: 241 QRFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHVVNI 300
           QRFVFRS+AILDLKAKAKSCDIPNPTCVE +TCFIWKYLMKVAD GDSQ PSTLSHVVNI
Sbjct: 282 QRFVFRSEAILDLKAKAKSCDIPNPTCVETLTCFIWKYLMKVADDGDSQRPSTLSHVVNI 341

Query: 301 RKMIEPSLGEVCLGNIMWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLKKINKDYIKE 360
           RKM+EPSLGEV LGNIMWGTVAHHFSTTR NE FEGLELSKLVSLLRQS KKINKDYIKE
Sbjct: 342 RKMLEPSLGEVSLGNIMWGTVAHHFSTTR-NEEFEGLELSKLVSLLRQSFKKINKDYIKE 401

Query: 361 LIMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNEVDFGWGKPLWFGIAGDSNE 420
           LIMGGDK RRNGVMKLVGEINKWPISNYYFFTSWKN+KLNEVDFGWGKPLW  IAGD NE
Sbjct: 402 LIMGGDKERRNGVMKLVGEINKWPISNYYFFTSWKNLKLNEVDFGWGKPLWSAIAGDPNE 461

Query: 421 MMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSIHLPHDHKIADQI 480
           MMGNIIVLVDNV DDGS EAWILLDEKEMQLLEQ PQFLEFALLNPSI+LPHD K AD+I
Sbjct: 462 MMGNIIVLVDNVLDDGSTEAWILLDEKEMQLLEQIPQFLEFALLNPSINLPHDQKTADEI 520

Query: 481 FSRKLI 486
           FS KLI
Sbjct: 522 FSNKLI 520

BLAST of CSPI04G03660 vs. ExPASy TrEMBL
Match: Q5I2Q4 (Putative alcohol acyl-transferases OS=Cucumis melo OX=3656 GN=AAT4 PE=2 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 1.0e-247
Identity = 435/486 (89.51%), Postives = 447/486 (91.98%), Query Frame = 0

Query: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60
           MEVKVLSKETIIPSSPTPPHLQ   LSLLDQLSPMLYIPLLLFYPMK+SY H     QD 
Sbjct: 1   MEVKVLSKETIIPSSPTPPHLQPLNLSLLDQLSPMLYIPLLLFYPMKKSYQH-----QDH 60

Query: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL 120
            K IATLKTSLSKTLSRFYLLAGRII KSIHCNDKGAVFMEATIN+NMFDILKEPNNEVL
Sbjct: 61  NKAIATLKTSLSKTLSRFYLLAGRIIGKSIHCNDKGAVFMEATINSNMFDILKEPNNEVL 120

Query: 121 TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN 180
           TKLLPCSLLCNTKPIEEYPQIVVQAN+F+CGGIAISLCLLHKLIDAATFCCFLRSWATTN
Sbjct: 121 TKLLPCSLLCNTKPIEEYPQIVVQANIFECGGIAISLCLLHKLIDAATFCCFLRSWATTN 180

Query: 181 RELLSQLDHSSP-NNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRRL 240
           RELLS LDHSSP NNMVC DYKSFSSLFPQTNLLPFHQ  INND AV+ PSSIFN KRR 
Sbjct: 181 RELLS-LDHSSPNNNMVCVDYKSFSSLFPQTNLLPFHQSLINNDKAVVPPSSIFNRKRRF 240

Query: 241 QRFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHVVNI 300
           QRFVFRS+AILDLKAKAKSCDIPNPTCVE +TCFIWKYLMKVAD GDSQ PSTLSHVVNI
Sbjct: 241 QRFVFRSEAILDLKAKAKSCDIPNPTCVETLTCFIWKYLMKVADDGDSQRPSTLSHVVNI 300

Query: 301 RKMIEPSLGEVCLGNIMWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLKKINKDYIKE 360
           RKM+EPSLGEV LGNIMWGTVAHHFSTTR NE FEGLELSKLVSLLRQS KKINKDYIKE
Sbjct: 301 RKMLEPSLGEVSLGNIMWGTVAHHFSTTR-NEEFEGLELSKLVSLLRQSFKKINKDYIKE 360

Query: 361 LIMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNEVDFGWGKPLWFGIAGDSNE 420
           LIMGGDK RRNGVMKLVGEINKWPISNYYFFTSWKN+KLNEVDFGWGKPLW  IAGD NE
Sbjct: 361 LIMGGDKERRNGVMKLVGEINKWPISNYYFFTSWKNLKLNEVDFGWGKPLWSAIAGDPNE 420

Query: 421 MMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSIHLPHDHKIADQI 480
           MMGNIIVLVDNV DDGS EAWILLDEKEMQLLEQ PQFLEFALLNPSI+LPH+ K AD+I
Sbjct: 421 MMGNIIVLVDNVLDDGSTEAWILLDEKEMQLLEQIPQFLEFALLNPSINLPHNQKTADEI 479

Query: 481 FSRKLI 486
           FS KLI
Sbjct: 481 FSNKLI 479

BLAST of CSPI04G03660 vs. ExPASy TrEMBL
Match: A0A6J1GVT5 (salutaridinol 7-O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111458004 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 1.1e-164
Identity = 308/469 (65.67%), Postives = 369/469 (78.68%), Query Frame = 0

Query: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60
           MEVKVLS+ETIIPSSPTPPHL+TF+LSLLDQLSPMLY PLL FYP+ ++Y        DP
Sbjct: 1   MEVKVLSRETIIPSSPTPPHLKTFELSLLDQLSPMLYTPLLFFYPITKTY--------DP 60

Query: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL 120
             T+ATLK SLS+TL+RFYLLAGRI+ KSIHC+D+GAVF+EA+++ NM  ILK+PNNEVL
Sbjct: 61  NPTMATLKDSLSQTLTRFYLLAGRIVGKSIHCSDQGAVFIEASVSCNMSHILKQPNNEVL 120

Query: 121 TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN 180
            KLLPCSLLC + P EE PQ+VVQAN+F CGGIAISLCLLHKL+D ATF  FL SWA+TN
Sbjct: 121 RKLLPCSLLC-SNPAEESPQVVVQANIFSCGGIAISLCLLHKLMDGATFTSFLTSWASTN 180

Query: 181 RELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRRLQ 240
           +     LD SS  NM+C+DYK+FSSLFPQTNLLPFHQ+       +  P+   N KR L+
Sbjct: 181 K---GSLDSSS--NMICYDYKAFSSLFPQTNLLPFHQK-------LAKPA---NRKRCLR 240

Query: 241 RFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHVVNIR 300
           RFVF SK++L+LKAKA+S  +PNP+CVEA+T FIWK+ M  A   D Q  STLSHVVN+R
Sbjct: 241 RFVFGSKSLLNLKAKAESRAVPNPSCVEALTGFIWKHAMMAA---DCQGSSTLSHVVNLR 300

Query: 301 KMIEPSLGEVCLGNIMWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLKKINKDYIKEL 360
             ++P+L EV +GNIMWG VAH       + A E LEL  LV LLRQS ++INKDYI+ +
Sbjct: 301 GRMKPALPEVSVGNIMWGAVAH-------SSAMERLELRALVGLLRQSFEEINKDYIERM 360

Query: 361 IMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNEVDFGWGKPLWFGIAGDSNEM 420
           ++G DKG    V+K VGE+NKW +SNYYF TSW+NMKLNEVDFGWG+P W  IA D+NE+
Sbjct: 361 LIGSDKG-FEAVLKFVGEMNKWGMSNYYFCTSWRNMKLNEVDFGWGRPAWIAIAWDTNEL 420

Query: 421 MGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSIHL 470
           M N+IVLVD V  DG IEAWILLDEKEM++LEQNPQFLEFALLNPSI+L
Sbjct: 421 MANVIVLVDGVMGDG-IEAWILLDEKEMEILEQNPQFLEFALLNPSIYL 433

BLAST of CSPI04G03660 vs. ExPASy TrEMBL
Match: A0A6J1GVI6 (salutaridinol 7-O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111457861 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 3.2e-164
Identity = 307/473 (64.90%), Postives = 371/473 (78.44%), Query Frame = 0

Query: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60
           MEVKVLS+ETIIPSSPTPPHL++F+LSLLDQLSPMLY PLL FYP+ ++Y        DP
Sbjct: 1   MEVKVLSRETIIPSSPTPPHLKSFKLSLLDQLSPMLYTPLLFFYPITKTY--------DP 60

Query: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL 120
             T+ATLK SLS+TL+RFYLLAGRI+ KSIHC+D+GAVF+EA+++ NM  ILK+PNNEVL
Sbjct: 61  NPTMATLKDSLSQTLARFYLLAGRIVGKSIHCSDQGAVFIEASVSCNMSHILKQPNNEVL 120

Query: 121 TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN 180
            KLLPCSLLC + P EE PQ+VVQAN+F CGGIAISLCLLHKL+D ATF  FL SWA+TN
Sbjct: 121 RKLLPCSLLC-SNPAEESPQVVVQANIFSCGGIAISLCLLHKLMDGATFTSFLTSWASTN 180

Query: 181 RELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRRLQ 240
           +     LD SS  NM+C+DYK+FSSLFPQTNLLPFHQ+       +  P+   N KR L+
Sbjct: 181 K---GSLDSSS--NMICYDYKAFSSLFPQTNLLPFHQK-------LAKPA---NRKRCLR 240

Query: 241 RFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHVVNIR 300
           RFVF SK++L+LKAKA+S  +PNP+CVEA+T FIWK+ M  A   D Q  STLSHVVN+R
Sbjct: 241 RFVFGSKSLLNLKAKAESRAVPNPSCVEALTGFIWKHAMMAA---DCQGSSTLSHVVNLR 300

Query: 301 KMIEPSLGEVCLGNIMWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLKKINKDYIKEL 360
             ++P+L EV +GNIMWG VAH       + A E LEL  LV LLRQS ++INKDYI+ +
Sbjct: 301 GRMKPALPEVSVGNIMWGAVAH-------SSAMERLELRALVGLLRQSFEEINKDYIERM 360

Query: 361 IMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNEVDFGWGKPLWFGIAGDSNEM 420
           ++G DKG    V+K VGE+NKW +SNYYF TSW+NMKLNEVDFGWG+P W  IA D+NE+
Sbjct: 361 LIGSDKG-FEAVLKFVGEMNKWGMSNYYFCTSWRNMKLNEVDFGWGRPAWIAIAWDTNEL 420

Query: 421 MGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSIHLPHDH 474
           M N+IVLVD V  DG IEAWILLDEKEM++LEQNPQFLEFALLNPSI+L  ++
Sbjct: 421 MANVIVLVDGVMGDG-IEAWILLDEKEMEILEQNPQFLEFALLNPSIYLADEN 437

BLAST of CSPI04G03660 vs. NCBI nr
Match: XP_004152093.1 (stemmadenine O-acetyltransferase [Cucumis sativus])

HSP 1 Score: 983.0 bits (2540), Expect = 9.1e-283
Identity = 481/485 (99.18%), Postives = 483/485 (99.59%), Query Frame = 0

Query: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60
           MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP
Sbjct: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60

Query: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL 120
           KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL
Sbjct: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL 120

Query: 121 TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN 180
           TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN
Sbjct: 121 TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN 180

Query: 181 RELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRRLQ 240
           RELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQR INNDNAVILPSSIFNGKRRLQ
Sbjct: 181 RELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRLINNDNAVILPSSIFNGKRRLQ 240

Query: 241 RFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHVVNIR 300
           RFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHVVNIR
Sbjct: 241 RFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHVVNIR 300

Query: 301 KMIEPSLGEVCLGNIMWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLKKINKDYIKEL 360
           KMIEPSLGEV LGNIMWGTVAHHFSTTRPNE+FEGLELSKLVSLLRQSLKKINKDYIKEL
Sbjct: 301 KMIEPSLGEVSLGNIMWGTVAHHFSTTRPNESFEGLELSKLVSLLRQSLKKINKDYIKEL 360

Query: 361 IMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNEVDFGWGKPLWFGIAGDSNEM 420
           IMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNE+DFGWGKPLWFGIAGDSNEM
Sbjct: 361 IMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNELDFGWGKPLWFGIAGDSNEM 420

Query: 421 MGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSIHLPHDHKIADQIF 480
           MGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSIHLPHDHKIADQIF
Sbjct: 421 MGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSIHLPHDHKIADQIF 480

Query: 481 SRKLI 486
           SRKLI
Sbjct: 481 SRKLI 485

BLAST of CSPI04G03660 vs. NCBI nr
Match: KAE8649121.1 (hypothetical protein Csa_015082 [Cucumis sativus])

HSP 1 Score: 941.0 bits (2431), Expect = 4.0e-270
Identity = 461/465 (99.14%), Postives = 463/465 (99.57%), Query Frame = 0

Query: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60
           MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP
Sbjct: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60

Query: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL 120
           KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL
Sbjct: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL 120

Query: 121 TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN 180
           TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN
Sbjct: 121 TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN 180

Query: 181 RELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRRLQ 240
           RELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQR INNDNAVILPSSIFNGKRRLQ
Sbjct: 181 RELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRLINNDNAVILPSSIFNGKRRLQ 240

Query: 241 RFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHVVNIR 300
           RFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHVVNIR
Sbjct: 241 RFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHVVNIR 300

Query: 301 KMIEPSLGEVCLGNIMWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLKKINKDYIKEL 360
           KMIEPSLGEV LGNIMWGTVAHHFSTTRPNE+FEGLELSKLVSLLRQSLKKINKDYIKEL
Sbjct: 301 KMIEPSLGEVSLGNIMWGTVAHHFSTTRPNESFEGLELSKLVSLLRQSLKKINKDYIKEL 360

Query: 361 IMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNEVDFGWGKPLWFGIAGDSNEM 420
           IMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNE+DFGWGKPLWFGIAGDSNEM
Sbjct: 361 IMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNELDFGWGKPLWFGIAGDSNEM 420

Query: 421 MGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNP 466
           MGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNP
Sbjct: 421 MGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNP 465

BLAST of CSPI04G03660 vs. NCBI nr
Match: XP_008453948.1 (PREDICTED: LOW QUALITY PROTEIN: salutaridinol 7-O-acetyltransferase-like [Cucumis melo])

HSP 1 Score: 868.2 bits (2242), Expect = 3.3e-248
Identity = 436/486 (89.71%), Postives = 447/486 (91.98%), Query Frame = 0

Query: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60
           MEVKVLSKETIIPSSPTPPHLQT QLSLLDQLSPMLYIPLLLFYPMK+SY H     QD 
Sbjct: 42  MEVKVLSKETIIPSSPTPPHLQTLQLSLLDQLSPMLYIPLLLFYPMKKSYQH-----QDH 101

Query: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL 120
            K IATLKTSLSKTLSRFYLLAGRII KSIHCNDKGAVFMEATIN+NMFDILKEPNNEVL
Sbjct: 102 NKAIATLKTSLSKTLSRFYLLAGRIIGKSIHCNDKGAVFMEATINSNMFDILKEPNNEVL 161

Query: 121 TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN 180
           TKLLPCSLLCNTKPIEEYPQIVVQAN+F+CGGIAISLCLLHKLIDAATFCCFLRSWATTN
Sbjct: 162 TKLLPCSLLCNTKPIEEYPQIVVQANIFECGGIAISLCLLHKLIDAATFCCFLRSWATTN 221

Query: 181 RELLSQLDHSSP-NNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRRL 240
           RE LS LDHSSP NNMVCFDYKSFSSLFPQTNLLPFHQ  INND  V+ PSSIFN KR  
Sbjct: 222 RESLS-LDHSSPNNNMVCFDYKSFSSLFPQTNLLPFHQSLINNDKXVVPPSSIFNRKRHF 281

Query: 241 QRFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHVVNI 300
           QRFVFRS+AILDLKAKAKSCDIPNPTCVE +TCFIWKYLMKVAD GDSQ PSTLSHVVNI
Sbjct: 282 QRFVFRSEAILDLKAKAKSCDIPNPTCVETLTCFIWKYLMKVADDGDSQRPSTLSHVVNI 341

Query: 301 RKMIEPSLGEVCLGNIMWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLKKINKDYIKE 360
           RKM+EPSLGEV LGNIMWGTVAHHFSTTR NE FEGLELSKLVSLLRQS KKINKDYIKE
Sbjct: 342 RKMLEPSLGEVSLGNIMWGTVAHHFSTTR-NEEFEGLELSKLVSLLRQSFKKINKDYIKE 401

Query: 361 LIMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNEVDFGWGKPLWFGIAGDSNE 420
           LIMGGDK RRNGVMKLVGEINKWPISNYYFFTSWKN+KLNEVDFGWGKPLW  IAGD NE
Sbjct: 402 LIMGGDKERRNGVMKLVGEINKWPISNYYFFTSWKNLKLNEVDFGWGKPLWSAIAGDPNE 461

Query: 421 MMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSIHLPHDHKIADQI 480
           MMGNIIVLVDNV DDGS EAWILLDEKEMQLLEQ PQFLEFALLNPSI+LPHD K AD+I
Sbjct: 462 MMGNIIVLVDNVLDDGSTEAWILLDEKEMQLLEQIPQFLEFALLNPSINLPHDQKTADEI 520

Query: 481 FSRKLI 486
           FS KLI
Sbjct: 522 FSNKLI 520

BLAST of CSPI04G03660 vs. NCBI nr
Match: NP_001315389.1 (salutaridinol 7-O-acetyltransferase-like [Cucumis melo] >AAW51126.1 putative alcohol acyl-transferases [Cucumis melo])

HSP 1 Score: 865.5 bits (2235), Expect = 2.1e-247
Identity = 435/486 (89.51%), Postives = 447/486 (91.98%), Query Frame = 0

Query: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60
           MEVKVLSKETIIPSSPTPPHLQ   LSLLDQLSPMLYIPLLLFYPMK+SY H     QD 
Sbjct: 1   MEVKVLSKETIIPSSPTPPHLQPLNLSLLDQLSPMLYIPLLLFYPMKKSYQH-----QDH 60

Query: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL 120
            K IATLKTSLSKTLSRFYLLAGRII KSIHCNDKGAVFMEATIN+NMFDILKEPNNEVL
Sbjct: 61  NKAIATLKTSLSKTLSRFYLLAGRIIGKSIHCNDKGAVFMEATINSNMFDILKEPNNEVL 120

Query: 121 TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN 180
           TKLLPCSLLCNTKPIEEYPQIVVQAN+F+CGGIAISLCLLHKLIDAATFCCFLRSWATTN
Sbjct: 121 TKLLPCSLLCNTKPIEEYPQIVVQANIFECGGIAISLCLLHKLIDAATFCCFLRSWATTN 180

Query: 181 RELLSQLDHSSP-NNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRRL 240
           RELLS LDHSSP NNMVC DYKSFSSLFPQTNLLPFHQ  INND AV+ PSSIFN KRR 
Sbjct: 181 RELLS-LDHSSPNNNMVCVDYKSFSSLFPQTNLLPFHQSLINNDKAVVPPSSIFNRKRRF 240

Query: 241 QRFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHVVNI 300
           QRFVFRS+AILDLKAKAKSCDIPNPTCVE +TCFIWKYLMKVAD GDSQ PSTLSHVVNI
Sbjct: 241 QRFVFRSEAILDLKAKAKSCDIPNPTCVETLTCFIWKYLMKVADDGDSQRPSTLSHVVNI 300

Query: 301 RKMIEPSLGEVCLGNIMWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLKKINKDYIKE 360
           RKM+EPSLGEV LGNIMWGTVAHHFSTTR NE FEGLELSKLVSLLRQS KKINKDYIKE
Sbjct: 301 RKMLEPSLGEVSLGNIMWGTVAHHFSTTR-NEEFEGLELSKLVSLLRQSFKKINKDYIKE 360

Query: 361 LIMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNEVDFGWGKPLWFGIAGDSNE 420
           LIMGGDK RRNGVMKLVGEINKWPISNYYFFTSWKN+KLNEVDFGWGKPLW  IAGD NE
Sbjct: 361 LIMGGDKERRNGVMKLVGEINKWPISNYYFFTSWKNLKLNEVDFGWGKPLWSAIAGDPNE 420

Query: 421 MMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSIHLPHDHKIADQI 480
           MMGNIIVLVDNV DDGS EAWILLDEKEMQLLEQ PQFLEFALLNPSI+LPH+ K AD+I
Sbjct: 421 MMGNIIVLVDNVLDDGSTEAWILLDEKEMQLLEQIPQFLEFALLNPSINLPHNQKTADEI 479

Query: 481 FSRKLI 486
           FS KLI
Sbjct: 481 FSNKLI 479

BLAST of CSPI04G03660 vs. NCBI nr
Match: XP_038875765.1 (BAHD acyltransferase BIA1-like [Benincasa hispida])

HSP 1 Score: 725.7 bits (1872), Expect = 2.6e-205
Identity = 371/472 (78.60%), Postives = 404/472 (85.59%), Query Frame = 0

Query: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60
           MEVKVLS+ETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLL FYPMK +YD ID   QDP
Sbjct: 1   MEVKVLSRETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLFFYPMKTNYD-IDH--QDP 60

Query: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL 120
           KKTIATLK SLSKTLSRFYLLAGRI DKSIHCNDKGAVF EAT++ NMF+ILKEPN+EVL
Sbjct: 61  KKTIATLKNSLSKTLSRFYLLAGRITDKSIHCNDKGAVFQEATVSCNMFEILKEPNSEVL 120

Query: 121 TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN 180
            KLLPCSL C TKPIEEY QIVVQAN+FKCGGIAISLCLLHKLIDAATF CFLRSWATTN
Sbjct: 121 AKLLPCSLSCTTKPIEEYTQIVVQANIFKCGGIAISLCLLHKLIDAATFSCFLRSWATTN 180

Query: 181 RELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRRLQ 240
           RELL   D S  NNM C+DY SFSSLFPQTNLLPFH+       A +  S+    K  LQ
Sbjct: 181 RELLLSFDSSPNNNMACYDYNSFSSLFPQTNLLPFHK-------AAVTLSTTPARKHHLQ 240

Query: 241 RFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHVVNIR 300
           RFVFRSKAILDLKAKAKS D+PNPTCVE +T FIWKY MKVAD  +SQ  STLSHVVN+R
Sbjct: 241 RFVFRSKAILDLKAKAKSSDVPNPTCVETLTGFIWKYAMKVADHDNSQKQSTLSHVVNMR 300

Query: 301 KMIEPSLGEVCLGNIMWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLKKI-NKDYIKE 360
           K+IEPSL E  +GNI+WGTVAH++STT  +E  E LELSKLVSLLRQS KK+ NK+YIKE
Sbjct: 301 KIIEPSLPEDSVGNILWGTVAHYYSTT-SHEEMEDLELSKLVSLLRQSFKKVNNKEYIKE 360

Query: 361 LIMGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNEVDFGWGKPLWFGIAGDSNE 420
           LIM  DKGR +GVMKLVGEINK P+SNYYFFTSWKNM LNEVDFGWGKPLWF IAGDSNE
Sbjct: 361 LIMESDKGRSDGVMKLVGEINKCPLSNYYFFTSWKNMNLNEVDFGWGKPLWFAIAGDSNE 420

Query: 421 MM-GNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSIHLP 471
           MM GN ++L+D+V DDGS+EAWILLDEK+MQLL+QN QFLEFALLNPSI+ P
Sbjct: 421 MMKGNFVILLDSVLDDGSMEAWILLDEKKMQLLDQNQQFLEFALLNPSINFP 461

BLAST of CSPI04G03660 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 246.9 bits (629), Expect = 3.3e-65
Identity = 161/483 (33.33%), Postives = 257/483 (53.21%), Query Frame = 0

Query: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60
           M V V+S++ I PSSPTP HL+ F+LSLL+QL P ++ P++ FY    S          P
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSI--------KP 60

Query: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDK-SIHCNDKGAVFMEATINTNMFDILKEPNNEV 120
            + +  LK SLS+TL+ FY LAGR+    SI CND GA F+EA +N+ + ++L EP+++ 
Sbjct: 61  TEQLQMLKKSLSETLTHFYPLAGRLKGNISIDCNDSGADFLEARVNSPLSNLLLEPSSDS 120

Query: 121 LTKLLPCSLLCNTKPIEEYPQIVV-QANVFKCGGIAISLCLLHKLIDAATFCCFLRSWAT 180
           L +L+P S+      IE   ++++ QA+ F+CG ++I +C+ HKL DA +   F++SWA 
Sbjct: 121 LQQLIPTSV----DSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAA 180

Query: 181 -TNRELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKR 240
            ++R  +  +     + +  F   +FS   P                A ++   I   + 
Sbjct: 181 ISSRGSIKTIGAPVFDTVKIFPPGNFSETSP----------------APVVEPEIMMNQT 240

Query: 241 RLQRFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVA-DGGDSQMPSTLSHV 300
             +RF+F S +I  L+AKA S ++  PT VEA++  IWK  MK       +  PS L++ 
Sbjct: 241 LSKRFIFDSSSIQALQAKASSFEVNQPTRVEAVSALIWKSAMKATRTVSGTSKPSILANS 300

Query: 301 VNIRKMIEPSLGEVCLGNIMWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLKKINKDY 360
           V++R  + P   +  +GN++       +   +  E     +L  LVS +R++ ++    +
Sbjct: 301 VSLRSRVSPPFTKNSIGNLV------SYFAAKAEEGINQTKLQTLVSKIRKAKQRFRDIH 360

Query: 361 IKELIMGGDKGRRNGV------MKLVGEINKWPISNYYFFTSWKNMKLNEVDFGWGKPLW 420
           I +L+     G  N         K  G++      ++Y F+S     L E DFGWGKP+W
Sbjct: 361 IPKLV-----GNPNATEIICSYQKEAGDMIASGDFDFYIFSSACRFGLYETDFGWGKPVW 420

Query: 421 FGIAGDSNEMMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSIHLP 474
            G      +   NI+ L+D   + G IEAW+ L+E+EM L EQ+ + L+FA LNPS+  P
Sbjct: 421 VGFPSVRQK---NIVTLLD-TKEAGGIEAWVNLNEQEMNLFEQDRELLQFASLNPSVIQP 440

BLAST of CSPI04G03660 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 233.0 bits (593), Expect = 5.0e-61
Identity = 169/488 (34.63%), Postives = 254/488 (52.05%), Query Frame = 0

Query: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60
           M+V+ +SKE I PSSPTP +LQT QLS+ D + P +Y    LFY      D I Q     
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKN---DLISQEHTSH 65

Query: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINT-NMFDILKEPNNEV 120
           K     LKTSLS+TL++FY LAGRI   ++ C D+GA+F++A +N   + + LK P+ + 
Sbjct: 66  K-----LKTSLSETLTKFYPLAGRITGVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDA 125

Query: 121 LTKLLPCSLLCNT-KPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWAT 180
           L +LLP  ++ N       +P ++V+A  F CGG+AI +C+ HK+ DAA+   F+RSWA 
Sbjct: 126 LQQLLPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAA 185

Query: 181 TNRELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRR 240
           T R           N+    +    S +F   N  P    P N   A  LP+    GKR 
Sbjct: 186 TAR---------GENDAAAME----SPVFAGANFYP----PAN--EAFKLPADEQAGKRS 245

Query: 241 --LQRFVFRSKAILDLKAKAKSCD-IPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSH 300
              +RFVF +  + DL+ KA S + +  PT VE++T  IWK  +       S   +T  H
Sbjct: 246 SITKRFVFEASKVEDLRTKAASEETVDQPTRVESVTALIWKCFVA------SSKTTTCDH 305

Query: 301 VV-----NIRKMIEPSLGEVCLGNIMWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLK 360
            V     N+R  I   L E  +GN+M+ +V    S  R  E        K+   +R   K
Sbjct: 306 KVLVQLANLRSKIPSLLQESSIGNLMFSSVV--LSIGRGGEV-------KIEEAVRDLRK 365

Query: 361 KINKDYIKELIMGGDKGRRNGVMKLVG-EINKWPISNY----------YFFTSWKNMKLN 420
           K  K+ +  +I+  D+G  +    ++G ++    ++NY          Y  +SW  + L 
Sbjct: 366 K--KEELGTVIL--DEGGSSDSSSMIGSKLANLMLTNYSRLSYETHEPYTVSSWCKLPLY 425

Query: 421 EVDFGWGKPLWFGIAGDSNEMMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLE 468
           E  FGW  P+W  + G+ + ++GN+ +L+D+  D   IEA++ L E+ M   EQNP+ L 
Sbjct: 426 EASFGWDSPVW--VVGNVSPVLGNLAMLIDS-KDGQGIEAFVTLPEENMSSFEQNPELLA 444

BLAST of CSPI04G03660 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 217.2 bits (552), Expect = 2.8e-56
Identity = 158/470 (33.62%), Postives = 242/470 (51.49%), Query Frame = 0

Query: 3   VKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDPKK 62
           V++LS+E + PSSPTP   +   LSLLD LS  +Y   LLFY    + D  +      ++
Sbjct: 5   VEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFY----AADPQNLLGFSTEE 64

Query: 63  TIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVLTK 122
           T   LK SLSKTL  FY LAGRII   + CND+GAVF+EA ++  + + LK P  E L  
Sbjct: 65  TSLKLKKSLSKTLPIFYPLAGRIIGSFVECNDEGAVFIEARVDHLLSEFLKCPVPESLEL 124

Query: 123 LLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTNRE 182
           L+P      ++    +P +++QAN F CGG+ I++C+ HK+ DA +   F+R WA ++R 
Sbjct: 125 LIPVE--AKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESSRG 184

Query: 183 LLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRRLQRF 242
           L           +      + S +FP+  L     +P++    V   S +       +RF
Sbjct: 185 L----------GITLIPSFTASEVFPKP-LDELPSKPMDRKEEVEEMSCV------TKRF 244

Query: 243 VFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVAD-GGDSQMPSTLSHVVNIRK 302
           VF +  I  L+AKA    + NPT VEA+T   W+ + KV+     +   S L  +VN+R 
Sbjct: 245 VFDASKIKKLRAKASRNLVKNPTRVEAVTALFWRCVTKVSRLSSLTPRTSVLQILVNLRG 304

Query: 303 MIEPSLGEVCLGNIMWGTVA--HHFSTTRPNEAFEGLELSKLVSLLRQSLKKINKDYIKE 362
            ++ SL E  +GN++   +      +  R  +  + +  +K +  L       +   I E
Sbjct: 305 KVD-SLCENTIGNMLSLMILKNEEAAIERIQDVVDEIRRAKEIFSLNCKEMSKSSSRIFE 364

Query: 363 LI--MGGDKGRRNGVMKLVGEINKWPISNYYFFTSWKNMKLNEVDFGWGKPLWFGIAGDS 422
           L+  +G   GR N       E++ W +SN     SW  + L + DFGWGKP+W  + G  
Sbjct: 365 LLEEIGKVYGRGN-------EMDLW-MSN-----SWCKLGLYDADFGWGKPVW--VTGRG 424

Query: 423 NEMMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSI 468
                N+++L+D    +G IEAWI L E++M L E + + LE A LNP +
Sbjct: 425 TSHFKNLMLLIDTKDGEG-IEAWITLTEEQMSLFECDQELLESASLNPPV 434

BLAST of CSPI04G03660 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 217.2 bits (552), Expect = 2.8e-56
Identity = 158/481 (32.85%), Postives = 248/481 (51.56%), Query Frame = 0

Query: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60
           M+V+ ++KE I PSS TP  LQT QLS+ D + P +Y    LFY    + D +   +Q  
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFY----TKDDLISPEQSS 63

Query: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINT-NMFDILKEPNNEV 120
            K    LKTSL++TL++FY LAGRI   +I CND+GAVF++A +N   + D L+ P+ + 
Sbjct: 64  HK----LKTSLAETLTKFYPLAGRIKGVTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKT 123

Query: 121 LTKLLPCSLLCNT-KPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWAT 180
           L +LLP  ++ +  +    +P ++V+A  F CGG+AI LC+ HK+ DA +   F++SWA 
Sbjct: 124 LQQLLPLDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAA 183

Query: 181 TNRELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGKRR 240
             R            ++V     + ++ +P  N L   + P++ +   I         + 
Sbjct: 184 MAR--------GEAGDIVADPVFAAANFYPPANEL--FKFPVDENANKI--------SQI 243

Query: 241 LQRFVFRSKAILDLKAKAKSCD-IPNPTCVEAITCFIWKYLMKVADGGDSQMP-STLSHV 300
            +RFVF +  + +L+ KA S D +  P  VE++T  +WK  +  A    +      L   
Sbjct: 244 TKRFVFSASKLEELRTKAASEDFVGRPKRVESVTALLWKAFVAAASSNTNACDMKVLIQP 303

Query: 301 VNIRKMIEPSLGEVCLGNIMWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLKKINKDY 360
            N+R  I   L E  +GNIM+ +V    S        E +++ K V  LR+    + ++ 
Sbjct: 304 SNLRPKIPSLLAESLIGNIMFSSVVLGISQQ------EEIKIEKAVRDLRKQGDDL-QNV 363

Query: 361 IKELIMGGDKGRRNGVMKLVGEINKWPISNY----------YFFTSWKNMKLNEVDFGWG 420
           IKE   GG      G      ++    ++NY          Y  +SW  + L E  FGWG
Sbjct: 364 IKE--EGGSSSAMIG-----SKLANLMLTNYSKLSYETHEPYTVSSWCKLPLYEASFGWG 423

Query: 421 KPLWFGIAGDSNEMMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPS 468
            P+W  IAG+   M+ N+ +L+D+  D   IEA++ L E+ M  LEQN + L FA +NPS
Sbjct: 424 SPVW--IAGNVAPMLENVTMLIDS-KDGQGIEAFVTLPEENMLSLEQNTELLAFASVNPS 441

BLAST of CSPI04G03660 vs. TAIR 10
Match: AT5G47950.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 216.1 bits (549), Expect = 6.3e-56
Identity = 154/475 (32.42%), Postives = 245/475 (51.58%), Query Frame = 0

Query: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60
           M+V+ + KE I PS+ TP  L T QLS++D L P +Y    LFY      D I Q     
Sbjct: 4   MKVETIGKEIIKPSATTPTDLSTLQLSIMDILMPPVYAVAFLFYTKD---DLISQ----- 63

Query: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATI-NTNMFDILKEPNNEV 120
           ++T  TLKTSLS+ L++F+ LAGR+   +I   D+GAVF+EA + N ++   L+ P+ E 
Sbjct: 64  EQTSHTLKTSLSEILTKFHPLAGRVNGVTIKSTDEGAVFVEARVDNCDLSGFLRSPDTES 123

Query: 121 LTKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATT 180
           L +LLP     + +P   +P ++V+A  F+CGG+AI LC+ H+L DAA+   FL++WA T
Sbjct: 124 LKQLLP----VDDEPAPTWPLLLVKATYFRCGGMAIGLCISHRLADAASLSIFLQAWAAT 183

Query: 181 NR---ELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRPINNDNAVILPSSIFNGK 240
            R   +L++  D  S            + L+P  N             A+ +P  + N  
Sbjct: 184 ARGESDLVASPDFCS------------TKLYPAAN------------EAIKIPGEVVNRT 243

Query: 241 RRLQRFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGDSQMPSTLSHV 300
              +RFVF +  I +L+ K  S  +P PT V+++T  IWK +  V    D+     L   
Sbjct: 244 SVTKRFVFVASKIEELRNKVASDVVPRPTRVQSVTSLIWKCV--VTASTDTIREKALFQP 303

Query: 301 VNIRKMIEPSLGEVCLGNIMWGTVAHHFSTTRPNEAFEGLELSKLVSLLRQSLKKINKDY 360
            N+R  I   L E  +GN+++ ++          +   G+++ + V    + L+K  +D 
Sbjct: 304 ANLRTKIPSLLSENQIGNLLFNSLI--------LDGKAGMDIVETV----KELQKRAED- 363

Query: 361 IKELIMGGDKGRRNGVMKLVGEINKWPIS----NYYFFTSWKNMKLNEVDFGWGKPLWFG 420
           +  L+   +        +L GEI     +    + +  TSW  + L +  FGWG P+W  
Sbjct: 364 LSGLVQDEEGSSMTIGWRLFGEIINSKFNREDHDMHSVTSWCKIPLYDACFGWGSPVW-- 423

Query: 421 IAGDSNEMMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSI 468
           +AG  +  +  + VL+D+  D   IEAW+ L ++ M LLEQ+ + L FA  NPS+
Sbjct: 424 VAGSVSPNLDYVTVLIDS-KDGQGIEAWVTLPQENMLLLEQSTELLAFASPNPSV 424

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94FT41.2e-4831.32Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 ... [more]
O233936.2e-4831.50BAHD acyltransferase BIA1 OS=Arabidopsis thaliana OX=3702 GN=BIA1 PE=2 SV=1[more]
I3PLR45.2e-4732.21(13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver ... [more]
A0A2P1GIW71.2e-4631.37Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1[more]
Q70PR72.3e-4230.13Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KXH24.4e-28399.18Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G016510 PE=3 SV=1[more]
A0A1S3BXK41.6e-24889.71LOW QUALITY PROTEIN: salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo OX... [more]
Q5I2Q41.0e-24789.51Putative alcohol acyl-transferases OS=Cucumis melo OX=3656 GN=AAT4 PE=2 SV=1[more]
A0A6J1GVT51.1e-16465.67salutaridinol 7-O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1GVI63.2e-16464.90salutaridinol 7-O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_004152093.19.1e-28399.18stemmadenine O-acetyltransferase [Cucumis sativus][more]
KAE8649121.14.0e-27099.14hypothetical protein Csa_015082 [Cucumis sativus][more]
XP_008453948.13.3e-24889.71PREDICTED: LOW QUALITY PROTEIN: salutaridinol 7-O-acetyltransferase-like [Cucumi... [more]
NP_001315389.12.1e-24789.51salutaridinol 7-O-acetyltransferase-like [Cucumis melo] >AAW51126.1 putative alc... [more]
XP_038875765.12.6e-20578.60BAHD acyltransferase BIA1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G26040.13.3e-6533.33HXXXD-type acyl-transferase family protein [more]
AT4G15390.15.0e-6134.63HXXXD-type acyl-transferase family protein [more]
AT1G24420.12.8e-5633.62HXXXD-type acyl-transferase family protein [more]
AT3G30280.12.8e-5632.85HXXXD-type acyl-transferase family protein [more]
AT5G47950.16.3e-5632.42HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003480TransferasePFAMPF02458Transferasecoord: 1..457
e-value: 2.3E-75
score: 253.9
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 1..214
e-value: 5.4E-55
score: 188.0
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 238..459
e-value: 4.0E-46
score: 158.8
NoneNo IPR availablePANTHERPTHR31623F21J9.9coord: 5..467
NoneNo IPR availablePANTHERPTHR31623:SF59HXXXD-TYPE ACYL-TRANSFERASE FAMILY PROTEINcoord: 5..467

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G03660.1CSPI04G03660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups