CSPI04G01030 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G01030
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionFumarate hydratase
LocationChr4: 552107 .. 557906 (-)
RNA-Seq ExpressionCSPI04G01030
SyntenyCSPI04G01030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCCATTTCTCTTTTGAATCTGTATTTTGTTCGTCCAAATAAATCGCCGTATTCTTTCACGACACCTACCACCTTCTGACGTCACCTGCCATTTCTCCGGCGTCTTATCTCCGTCCATTCCTCCGTTCGAGGATTCCACTTTCCCCAATCTCCATTCTTATTTGTTACTCTTCTCTCCTCCTCTTTCTGGATCACTCTCTCATTCTTCCCCAAACTCATATTAGCTTTTACCTCTAAAAAATGGCGGCCGTCTATGTTTTGTCCCGGCGATCTTCCGCTTCACACCTCTTTGGGATGGCTCGTTATGCTACTACGTCACTGAGATCTTACTCCACGTCTTTTCGCGAGGAAAGGGATACTTTTGGACCTATCAACGTTCCCTCCGATAGGTAGTTTGTCTTGTTTTTGGATTTACTTGACTAATAATTGCTTTGTGTTCGTTGTGTTTTAAATTATGGGGAGCTGTGATAAGTGAAGTGAAATGAGTTTTTATGAATTTTTTGTTGTGTTTTAGATTATGGGGAGCTCAAACGCAGAGATCGTTGCAGAATTTTGAGATCGGAGGCGACCGGGAGCGAATGCCAGAACCTATAATTCGTGCCTTTGGCGTTCTTAAAAAGTGTGCTGCCAAGGTCCATTCGTTTTCAAATTAAGTACAATAGTTCGTGTTTTGCAACTGAGATAAACGTGGAATACCGTTTTGGAAATAGTTTCTTATTCTCTTGCTCATTGTGAGTCTGTTTTTGTAGGTAAACATGGACTACGGTCTTGATCCAACAATTGGGAAAGCCATAATGCAAGCAGCTCAAGAAGTAGCGGAGGGAAAGTTGAATGATCATTTTCCACTTGTTGTATGGCAAACTGGAAGTGGTACTCAGAGTAACATGAATGCGAATGAGGTTGGAACAACTCTGTTTTTATTTTGATTATGAAGCTTCGAGAGTGAAGGATTGTACGTAGTTAGCATATTTTAGGATTTTTCACGAATCATTTTCTTTGGAGAAGTTATTGAGGCAGGCGTCACAATGTGTTTTCCATCCTTGATAGTTTTTCCTTTTTTTATCGAGATGTGTGGGACTCTGGTTACCATCTAAATGCTTTCCAGAGGCATGGAAGCTTATGTCACGCTTCTTCGTCGTTTTTGCGTTCTTATAAACAGCAGGACGGCAGGTGTACTAACTCATAGTAACCAGAGAAATGATTTGCTTCTCTTGGGTAGTGAGCTTCAGTCAGTGTTCGGATTTACTTTGCCCCTAAACTCGATTGCTAAAATTTATTGTACAATGTTACGTGAAGTAGGCAGCTTCAAAACTAGTGGTCAATAATGGATGAATTTAAAATGGTGTAAGATGACAATAGTCCTTCCCCTCTTCCTCTTCTTCATCGTGTCAAATTCATGCATTCTCTAAGTATCATCTCTTGTAAAATAAAATCCTTTATTAACCTTCATTAAAATATTTGAAGTGCCCCATAAGAATGTTTTGTGCCCATAATAGATGAAGATGTATATTATGGTAATCTGTAAAGTAATTTTTTTAGAATGCCCCTTAGGGTCTACTTTGTTGGTTGATTTGAATGTTAGAAAATACGAACCTCTACTGCACAATTGACGAACATCTTACTTTTGTGGCAGGTTATTGCTAACAGAGCATCTGAGATTCTTGGCCACAAACGTGGTGATAAGTTTGTTCACCCAAATGACCATGTTAACCGGTCCCAGTCTTCTAATGATACATTCCCAACTGTAAGATTTTGATTTACCAAGTTAATTTGTCAATTGCTTGCTATTCTAACTCTAATTCTATAACTAACAAAAACCAAGTTTAGCAGTTTTCCATGGCCAAGCTTCTTTTTGTTTAGTAATTTTAATAGTCAAACTTTTGTCTTTATTACTTTGACCAAGAAACAACTTTTTTTTTGTCTCAATTGACTAATTGTTTTTCTCCACTTCTGATTTCTACTGTTTTCCTTTCGCAGGTAATGCACATAGCAGCTGCAACAGAAATTAATTCCAAATTCATACCAAAATTGAAAACTTTGCATTCATCACTATACTCAAAGGTTTGATGTTTAGGAGGCTATGCTGCCTAAAATTTCAATTTCATAGTTTGCAAAAGTCTCTTTAATTCTATGCTTGTTTGATTGCATGGGCTTAGCTGAATGTATAAAAGTGGGAAAACATTACAAGATCCACATTGTTTGGATCTTATTGTACCATCCTAGGTTTAGCACATTTCAATTGAGTTGTGTGCTAGTAATGTATGTGTTTATTTACACTCCAAGCTCTTGCCTTGCATTCTATTTTCCCTTTGCACTTTCATAAGTTTGTAATTCCTTGTATTTCTCTGTTATGATCCTAACAGTCTATAGAATTCAAAGATATCGTAAAAATTGGACGCACTCATACTCAAGATGCCACACCTCTAACTCTTGGACAAGAGTTCAGTGGCTATACCACACAAGTATGCCTTCTTTCTCTATGTGCTTGTGGAAGTTCTGGGTTATTATGTCTTTCGAAAAGAAGAGGGTTTAACGAATTGCATATGAGTAATTTTCTTATTGGTATGCGCTTGGACTGCAGGTGAAGTATGGTATTGATAGAGTCTTGTGTACTCTACCTCGAATGTATCAGGTTGGAAAACGTGTGATTTTCCCAGATGTTTTGTCTCTTCTTTTTTTTTTTTTTTTTGACACTTCAACATTCCATTTGTGCAGCTTGCCCAAGGTGGAACGGCTGTTGGAACTGGATTGAACACAAAGAAAGGGTATAATTCCTTTTAGTTAGTTTGGATAAGGAAAACTATCTTGTTTTTGGATATTCAGCTTGATGATAAGAAGGCTGAACGTGTTTCTCATTTTAGATTGGTGTTATCTATTGTTCAGATTTGATGTGAAAATAGCGGCAGCAGTAGCTGAAGAAACAAATTTGCCATTTGTGACAGCAGAAAACAAGTTCGAAGCCTTGGTTAGTCATTGGATGAGCTTCATCCTTTTATTCCACAATTTGGGGTTTCTATCCTTCACACATGGAACCACTTTTTTTCTACTAGGCTGCACATGATGCCTTCGTTGAAACTAGTGGATCTCTTAACACAGTTGCTGCTTCTTTGATGAAGATTGCCAATGACATACGCCTGTTGGGAAGGTTGTATTAGTTTTGTCAATCTTTCTCTTTGTTATTTTTTCTTTCTTTCTAACATTTTCTCCTTTCATTTCATTTCAAGTATACAGTGGTCCACGTTGTGGTCTTGGTGAACTTATTCTTCCCGAAAACGAACCAGGCAGTAGTATTATGCCAGTTACTTTCTCTCTCTCTCTCCTTGCATATATATTTGGTGGCATGTGCATTCTGGGTTTTCGTAACCTAAACATTTCTTTTTTGTTTCAGGGTAAGGTGAACCCTACTCAATGTGAGGCTCTCACTATGGTCTGCGCTCAGGTAAATAATATCTTGCGTAGTTTATGGGGTGCTGTAAGAGGAATTAATTAACATAGATTCAGTCAACTCATTATGTTTGTTTCCTTTTGTTCCACAAATTCTTTTGGCTAATTTCCATGAGCCTACGCGTACTCAGATTCTTAGTGATTATTTCATGTGTATTGTTTTGTCATTCTAGAATTTAAAAGATGATATGGCAACTCGCATCGTTTATATGCAGGTTATGGGAAACCATGTGGCAATAACTGTTGGTGGGTCAAATGGTCACTTTGAACTTAATGTATATAAACCAATGATAGCCAATGCTCTTTTGCATGTAAGTTCCATCATCATCTCTTCACCGTTACTATGGCTAGGGATTACGTTCAAATAATGATATCAGAAACTAAACAGTTAAAATAAAAATTCTGGATTCATCGAATTTCATCTCCAATGCTGGTATATCATTGGCAGTCATTGAGATTATTAGGGGATGCTTCTGCTTCATTTGAGAAGAACTGTGTGAGGGGAATTCAAGCTAACAGGGAAAGAATTTCGAAATTACTCCATGAGGTAAATTTTCTACTATTATTTGATAAACACTTATATACTACACTGTTGAATAGAAGATTTTTGGCTGATGATTCTTGTGGTTATCTCTTCGTGTAAATCCGATGGCCCCTTCAGTCTCTGATGCTCGTCACAGCTTTAAACCCTGTAAGTGTTCATCAACTAGAAAGCTCTCGTTTTCCTTAAGCTTGGTATTTTGTTTGCTAATATATGTCCCTGGCTTATTGCCCAGAAAATTGGTTACGACAACGCTGCTGCAGTTGCAAAGAAAGCTCACAAGGAAGGGAGTACTTTGAAGGTATGAATTTTATTTATATTTAGTTTTATTTATATTGGGGGTAGGAGAGCCAAACTATTTAAGTACCGCACTGATTATCCCATTCTAACTAATGTGGGACAAGGACATCTAAACTACGGTGGTTCCTAACAATATTCTATCCCCAGAAAACCAATGTCCTGACAGCTACTTCAGTGAAACCTCACTAGGCCACACCCTGTAGAACCCTCTGTCAATTCCACTTCAAAATGTCGACAACCGTATTTTATTATTAGATACTTAAGCACCTTGGAGAACTACACACCTATATTAAGGGGAAAAAACAAACTTATACATGATGTCATATGAGTGAATAAGATGTCTACATGAAAATGTCTAGGGTTTGTTATATGAGTGAATAAGATATCTACATGGAGAAAAATTTAATGTTTTTTTATAATTAAAAAGATGTCTACATGAACAAGTACTTGAAAGTCAAATGCTGTATGTTAGGTGTCAAGGCTTCATATATGCTTGGGTGTTTAGATTCTTGTTGAACGATTAAAGAAAAATTAACTGGGATTATATTAGCATTTTAATGTCAAATATAGAAATAAGAACAAACCATTGAAAGTTAAAAGGGACTGAGAGAAATTATACCACTTATTAGAAATTTAGTCCATGGTTGAGGTTTGTACTTGTTTTGTCCCTAAATTTTCAAGTTTTTTTTTTTTAAAAAAAAATCAGTCTACTAAATACAAATTGAAAATCTAGGATTTCATTAGACATAAAATTCAAATTATCTAATAGAGCAATCAATTTTTTAGATCAAAATTGAAGTTCAGGGACTCAGGTTTGGAACAAAAAAGAAACAAACTTACATTGAAGAACTATACTTGGAGACCAAATAAAACATATATGATACGATACACAGACCACCAAAATAAGGATTGAAACTTAAATTTGATTGGTTAATTGTGGTTTTGGTATTTGATAATGCAGGAAGCTGCACTGAAGCTTGGAGTTCTAAGCGAGGAAGATTTTGATAAACATGTGGTGCCCGAGAAAATGATTGGTCCATCCGATTGCTAATAGAGAGCTAAAAAGCTTCCTTCTTCTGTGTTTGGATCCATCAAAATCATTTTTATAGGTACTCTCATTTTTCCCCTAAAAGGAACAACAAATCCATTTTTACGAGGATTGAACTGGATAATAAAATGACCACACAGAAAGAGTGTCTGGCCGTTCTCTTCTACTTTAGAAAATATTGTCAAAAAGGTTTTCTTGCGGGAAGTAATTAATAATTTCTTTTGGAGACATTATTTCTTGTGTACGTCTACGTCCATTATTGGATCTCTTTGAGAATTTGATCATTTTTCCCCTTTTGAAAATGAAGAATATGACATTCATCAATAGGGGATTTGAGCCTCTACTTGTTTGTTCTAATCTTGTAGTGTTCAGTAGGGGCTAGACTTCAGTATTTTCTGAAAAAATAAAAGCATTATTGCACACATTTTATTATGCATACATATCTTAGGTATGGTATAATCACGAC

mRNA sequence

ATTCCATTTCTCTTTTGAATCTGTATTTTGTTCGTCCAAATAAATCGCCGTATTCTTTCACGACACCTACCACCTTCTGACGTCACCTGCCATTTCTCCGGCGTCTTATCTCCGTCCATTCCTCCGTTCGAGGATTCCACTTTCCCCAATCTCCATTCTTATTTGTTACTCTTCTCTCCTCCTCTTTCTGGATCACTCTCTCATTCTTCCCCAAACTCATATTAGCTTTTACCTCTAAAAAATGGCGGCCGTCTATGTTTTGTCCCGGCGATCTTCCGCTTCACACCTCTTTGGGATGGCTCGTTATGCTACTACGTCACTGAGATCTTACTCCACGTCTTTTCGCGAGGAAAGGGATACTTTTGGACCTATCAACGTTCCCTCCGATAGATTATGGGGAGCTCAAACGCAGAGATCGTTGCAGAATTTTGAGATCGGAGGCGACCGGGAGCGAATGCCAGAACCTATAATTCGTGCCTTTGGCGTTCTTAAAAAGTGTGCTGCCAAGGTAAACATGGACTACGGTCTTGATCCAACAATTGGGAAAGCCATAATGCAAGCAGCTCAAGAAGTAGCGGAGGGAAAGTTGAATGATCATTTTCCACTTGTTGTATGGCAAACTGGAAGTGGTACTCAGAGTAACATGAATGCGAATGAGGTTATTGCTAACAGAGCATCTGAGATTCTTGGCCACAAACGTGGTGATAAGTTTGTTCACCCAAATGACCATGTTAACCGGTCCCAGTCTTCTAATGATACATTCCCAACTGTAATGCACATAGCAGCTGCAACAGAAATTAATTCCAAATTCATACCAAAATTGAAAACTTTGCATTCATCACTATACTCAAAGTCTATAGAATTCAAAGATATCGTAAAAATTGGACGCACTCATACTCAAGATGCCACACCTCTAACTCTTGGACAAGAGTTCAGTGGCTATACCACACAAGTGAAGTATGGTATTGATAGAGTCTTGTGTACTCTACCTCGAATGTATCAGCTTGCCCAAGGTGGAACGGCTGTTGGAACTGGATTGAACACAAAGAAAGGATTTGATGTGAAAATAGCGGCAGCAGTAGCTGAAGAAACAAATTTGCCATTTGTGACAGCAGAAAACAAGTTCGAAGCCTTGGCTGCACATGATGCCTTCGTTGAAACTAGTGGATCTCTTAACACAGTTGCTGCTTCTTTGATGAAGATTGCCAATGACATACGCCTGTTGGGAAGTGGTCCACGTTGTGGTCTTGGTGAACTTATTCTTCCCGAAAACGAACCAGGCAGTAGTATTATGCCAGGTAAGGTGAACCCTACTCAATGTGAGGCTCTCACTATGGTCTGCGCTCAGGTTATGGGAAACCATGTGGCAATAACTGTTGGTGGGTCAAATGGTCACTTTGAACTTAATGTATATAAACCAATGATAGCCAATGCTCTTTTGCATTCATTGAGATTATTAGGGGATGCTTCTGCTTCATTTGAGAAGAACTGTGTGAGGGGAATTCAAGCTAACAGGGAAAGAATTTCGAAATTACTCCATGAGTCTCTGATGCTCGTCACAGCTTTAAACCCTAAAATTGGTTACGACAACGCTGCTGCAGTTGCAAAGAAAGCTCACAAGGAAGGGAGTACTTTGAAGGAAGCTGCACTGAAGCTTGGAGTTCTAAGCGAGGAAGATTTTGATAAACATGTGGTGCCCGAGAAAATGATTGGTCCATCCGATTGCTAATAGAGAGCTAAAAAGCTTCCTTCTTCTGTGTTTGGATCCATCAAAATCATTTTTATAGGTACTCTCATTTTTCCCCTAAAAGGAACAACAAATCCATTTTTACGAGGATTGAACTGGATAATAAAATGACCACACAGAAAGAGTGTCTGGCCGTTCTCTTCTACTTTAGAAAATATTGTCAAAAAGGTTTTCTTGCGGGAAGTAATTAATAATTTCTTTTGGAGACATTATTTCTTGTGTACGTCTACGTCCATTATTGGATCTCTTTGAGAATTTGATCATTTTTCCCCTTTTGAAAATGAAGAATATGACATTCATCAATAGGGGATTTGAGCCTCTACTTGTTTGTTCTAATCTTGTAGTGTTCAGTAGGGGCTAGACTTCAGTATTTTCTGAAAAAATAAAAGCATTATTGCACACATTTTATTATGCATACATATCTTAGGTATGGTATAATCACGAC

Coding sequence (CDS)

ATGGCGGCCGTCTATGTTTTGTCCCGGCGATCTTCCGCTTCACACCTCTTTGGGATGGCTCGTTATGCTACTACGTCACTGAGATCTTACTCCACGTCTTTTCGCGAGGAAAGGGATACTTTTGGACCTATCAACGTTCCCTCCGATAGATTATGGGGAGCTCAAACGCAGAGATCGTTGCAGAATTTTGAGATCGGAGGCGACCGGGAGCGAATGCCAGAACCTATAATTCGTGCCTTTGGCGTTCTTAAAAAGTGTGCTGCCAAGGTAAACATGGACTACGGTCTTGATCCAACAATTGGGAAAGCCATAATGCAAGCAGCTCAAGAAGTAGCGGAGGGAAAGTTGAATGATCATTTTCCACTTGTTGTATGGCAAACTGGAAGTGGTACTCAGAGTAACATGAATGCGAATGAGGTTATTGCTAACAGAGCATCTGAGATTCTTGGCCACAAACGTGGTGATAAGTTTGTTCACCCAAATGACCATGTTAACCGGTCCCAGTCTTCTAATGATACATTCCCAACTGTAATGCACATAGCAGCTGCAACAGAAATTAATTCCAAATTCATACCAAAATTGAAAACTTTGCATTCATCACTATACTCAAAGTCTATAGAATTCAAAGATATCGTAAAAATTGGACGCACTCATACTCAAGATGCCACACCTCTAACTCTTGGACAAGAGTTCAGTGGCTATACCACACAAGTGAAGTATGGTATTGATAGAGTCTTGTGTACTCTACCTCGAATGTATCAGCTTGCCCAAGGTGGAACGGCTGTTGGAACTGGATTGAACACAAAGAAAGGATTTGATGTGAAAATAGCGGCAGCAGTAGCTGAAGAAACAAATTTGCCATTTGTGACAGCAGAAAACAAGTTCGAAGCCTTGGCTGCACATGATGCCTTCGTTGAAACTAGTGGATCTCTTAACACAGTTGCTGCTTCTTTGATGAAGATTGCCAATGACATACGCCTGTTGGGAAGTGGTCCACGTTGTGGTCTTGGTGAACTTATTCTTCCCGAAAACGAACCAGGCAGTAGTATTATGCCAGGTAAGGTGAACCCTACTCAATGTGAGGCTCTCACTATGGTCTGCGCTCAGGTTATGGGAAACCATGTGGCAATAACTGTTGGTGGGTCAAATGGTCACTTTGAACTTAATGTATATAAACCAATGATAGCCAATGCTCTTTTGCATTCATTGAGATTATTAGGGGATGCTTCTGCTTCATTTGAGAAGAACTGTGTGAGGGGAATTCAAGCTAACAGGGAAAGAATTTCGAAATTACTCCATGAGTCTCTGATGCTCGTCACAGCTTTAAACCCTAAAATTGGTTACGACAACGCTGCTGCAGTTGCAAAGAAAGCTCACAAGGAAGGGAGTACTTTGAAGGAAGCTGCACTGAAGCTTGGAGTTCTAAGCGAGGAAGATTTTGATAAACATGTGGTGCCCGAGAAAATGATTGGTCCATCCGATTGCTAA

Protein sequence

MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRGIQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDFDKHVVPEKMIGPSDC*
Homology
BLAST of CSPI04G01030 vs. ExPASy Swiss-Prot
Match: P93033 (Fumarate hydratase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FUM1 PE=1 SV=2)

HSP 1 Score: 870.5 bits (2248), Expect = 8.9e-252
Identity = 437/492 (88.82%), Postives = 462/492 (93.90%), Query Frame = 0

Query: 3   AVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQN 62
           ++YV SRR S        RYA TSLRSYSTSFREERDTFGPI VPSD+LWGAQTQRSLQN
Sbjct: 2   SIYVASRRLSGGTTVTALRYA-TSLRSYSTSFREERDTFGPIQVPSDKLWGAQTQRSLQN 61

Query: 63  FEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHFPL 122
           FEIGG+RERMPEPI+RAFGVLKKCAAKVNM+YGLDPTIGKAIMQAAQEVAEGKLNDHFPL
Sbjct: 62  FEIGGERERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPL 121

Query: 123 VVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHIAA 182
           VVWQTGSGTQSNMNANEVIANRA+EILG KRG+K VHPNDHVNRSQSSNDTFPTVMHIAA
Sbjct: 122 VVWQTGSGTQSNMNANEVIANRAAEILGRKRGEKCVHPNDHVNRSQSSNDTFPTVMHIAA 181

Query: 183 ATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGI 242
           ATEINS+ IP LKTLHS+L SKS EFKDIVKIGRTHTQDATPLTLGQEF GY TQVKYG+
Sbjct: 182 ATEINSRLIPSLKTLHSTLESKSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVKYGL 241

Query: 243 DRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHD 302
           +RV CTLPR+YQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHD
Sbjct: 242 NRVTCTLPRLYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHD 301

Query: 303 AFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEA 362
           A VETSGSLNT+A SLMKIANDIR LGSGPRCGLGEL+LPENEPGSSIMPGKVNPTQCEA
Sbjct: 302 ACVETSGSLNTIATSLMKIANDIRFLGSGPRCGLGELVLPENEPGSSIMPGKVNPTQCEA 361

Query: 363 LTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRGIQ 422
           LTMVCAQVMGNHVA+TVGGSNGHFELNV+KP+IA+ALLHS+RL+ DASASFEKNCVRGI+
Sbjct: 362 LTMVCAQVMGNHVAVTVGGSNGHFELNVFKPVIASALLHSVRLIADASASFEKNCVRGIE 421

Query: 423 ANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDFDK 482
           ANRERISKLLHESLMLVT+LNPKIGYDNAAAVAKKAHKEG TLKEAAL LGVL+ E+FD 
Sbjct: 422 ANRERISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGCTLKEAALNLGVLTAEEFDT 481

Query: 483 HVVPEKMIGPSD 495
            VVPEKMIGPSD
Sbjct: 482 LVVPEKMIGPSD 492

BLAST of CSPI04G01030 vs. ExPASy Swiss-Prot
Match: Q9FI53 (Fumarate hydratase 2 OS=Arabidopsis thaliana OX=3702 GN=FUM2 PE=1 SV=1)

HSP 1 Score: 847.8 bits (2189), Expect = 6.1e-245
Identity = 418/467 (89.51%), Postives = 444/467 (95.07%), Query Frame = 0

Query: 28  RSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIIRAFGVLKKCA 87
           RSYST FREERDTFGPI VPSD+LWGAQTQRSLQNFEIGGDRERMPEPI+RAFGVLKKCA
Sbjct: 33  RSYSTPFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 92

Query: 88  AKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRASE 147
           AKVNM+YGLDP IG+AIM+AAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRA+E
Sbjct: 93  AKVNMEYGLDPMIGEAIMEAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRAAE 152

Query: 148 ILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSKFIPKLKTLHSSLYSKSIE 207
           ILGHKRG+K VHPNDHVNRSQSSNDTFPTVMHIAAATEI S+ IP LK LHSSL SKS E
Sbjct: 153 ILGHKRGEKIVHPNDHVNRSQSSNDTFPTVMHIAAATEITSRLIPSLKNLHSSLESKSFE 212

Query: 208 FKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLN 267
           FKDIVKIGRTHTQDATPLTLGQEF GY TQV+YG++RV CTLPR+YQLAQGGTAVGTGLN
Sbjct: 213 FKDIVKIGRTHTQDATPLTLGQEFGGYATQVEYGLNRVACTLPRIYQLAQGGTAVGTGLN 272

Query: 268 TKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDAFVETSGSLNTVAASLMKIANDIRL 327
           TKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDA VETSGSLNT+A SLMKIANDIR 
Sbjct: 273 TKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDACVETSGSLNTIATSLMKIANDIRF 332

Query: 328 LGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAITVGGSNGHFE 387
           LGSGPRCGLGEL LPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVA+T+GGSNGHFE
Sbjct: 333 LGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTIGGSNGHFE 392

Query: 388 LNVYKPMIANALLHSLRLLGDASASFEKNCVRGIQANRERISKLLHESLMLVTALNPKIG 447
           LNV+KP+IA+ALLHS+RL+ DASASFEKNCVRGI+ANRERISKLLHESLMLVT+LNPKIG
Sbjct: 393 LNVFKPVIASALLHSIRLIADASASFEKNCVRGIEANRERISKLLHESLMLVTSLNPKIG 452

Query: 448 YDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDFDKHVVPEKMIGPSD 495
           YDNAAAVAK+AHKEG TLK AA+KLGVL+ E+FD  VVPEKMIGPSD
Sbjct: 453 YDNAAAVAKRAHKEGCTLKHAAMKLGVLTSEEFDTLVVPEKMIGPSD 499

BLAST of CSPI04G01030 vs. ExPASy Swiss-Prot
Match: P55250 (Fumarate hydratase, mitochondrial OS=Rhizopus oryzae OX=64495 GN=FUMR PE=3 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 2.1e-192
Identity = 342/492 (69.51%), Postives = 392/492 (79.67%), Query Frame = 0

Query: 10  RSSASHLFGMARYATTSLRSYSTS------FREERDTFGPINVPSDRLWGAQTQRSLQNF 69
           R+SA+     A+    S R +S++      FR ERDTFG + VP+DR WGAQTQRSLQNF
Sbjct: 3   RASATRFLSQAKNMNNSPRLFSSASAALQKFRAERDTFGDLQVPADRYWGAQTQRSLQNF 62

Query: 70  EIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHFPLV 129
           +IGG  ERMPEP+IRAFGVLKK AA VNM YGLDP +G+AI +AA EV +G L DHFPLV
Sbjct: 63  DIGGPTERMPEPLIRAFGVLKKAAATVNMTYGLDPKVGEAIQKAADEVIDGSLIDHFPLV 122

Query: 130 VWQTGSGTQSNMNANEVIANRASEILGHKRGDKF-VHPNDHVNRSQSSNDTFPTVMHIAA 189
           VWQTGSGTQ+ MN NEVI+NRA E+LG + G K  VHPNDHVN SQSSNDTFPT MH+AA
Sbjct: 123 VWQTGSGTQTKMNVNEVISNRAIELLGGELGSKAPVHPNDHVNMSQSSNDTFPTAMHVAA 182

Query: 190 ATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGI 249
             EI+ + IP L TL  +L +KS EF+ I+KIGRTH QDATPLTLGQEFSGYT Q+ YGI
Sbjct: 183 VVEIHGRLIPALTTLRDALQAKSAEFEHIIKIGRTHLQDATPLTLGQEFSGYTQQLTYGI 242

Query: 250 DRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHD 309
            RV  TL R+Y LAQGGTAVGTGLNT+KGFD K+A A+A  T LPF TA NKFEALAAHD
Sbjct: 243 ARVQGTLERLYNLAQGGTAVGTGLNTRKGFDAKVAEAIASITGLPFKTAPNKFEALAAHD 302

Query: 310 AFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEA 369
           A VE  G+LNTVA SLMKIANDIR LGSGPRCGLGEL LPENEPGSSIMPGKVNPTQCEA
Sbjct: 303 ALVEAHGALNTVACSLMKIANDIRYLGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEA 362

Query: 370 LTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRGIQ 429
           +TMVCAQVMGN+ AI+V GSNG FELNV+KP++   L+ S+RL+ DAS SF KNCV GI+
Sbjct: 363 MTMVCAQVMGNNTAISVAGSNGQFELNVFKPVMIKNLIQSIRLISDASISFTKNCVVGIE 422

Query: 430 ANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDFDK 489
           AN ++IS +++ESLMLVTALNP IGYD AA  AKKAHKEG+TLKEAAL LG L+ E+FD+
Sbjct: 423 ANEKKISSIMNESLMLVTALNPHIGYDKAAKCAKKAHKEGTTLKEAALSLGYLTSEEFDQ 482

Query: 490 HVVPEKMIGPSD 495
            V PE MI   D
Sbjct: 483 WVRPEDMISAKD 494

BLAST of CSPI04G01030 vs. ExPASy Swiss-Prot
Match: Q60HF9 (Fumarate hydratase, mitochondrial OS=Macaca fascicularis OX=9541 GN=FH PE=2 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 5.1e-191
Identity = 333/461 (72.23%), Postives = 381/461 (82.65%), Query Frame = 0

Query: 33  SFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIIRAFGVLKKCAAKVNM 92
           SFR E DTFG + VP+D+ +GAQT RS  NF+IGG  ERMP P+I+AFG+LK+ AA+VN 
Sbjct: 49  SFRIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILKRAAAEVNQ 108

Query: 93  DYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRASEILGHK 152
           DYGLDP I  AIM+AA EVAEGKLNDHFPLVVWQTGSGTQ+NMN NEVI+NRA E+LG +
Sbjct: 109 DYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNEVISNRAIEMLGGE 168

Query: 153 RGDKF-VHPNDHVNRSQSSNDTFPTVMHIAAATEINSKFIPKLKTLHSSLYSKSIEFKDI 212
            G K  VHPNDHVN+SQSSNDTFPT MHIAAA E++   +P L+ LH +L +KS EF  I
Sbjct: 169 LGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDALDAKSKEFAQI 228

Query: 213 VKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKG 272
           +KIGRTHTQDA PLTLGQEFSGY  QVKY + R+   +PR+Y+LA GGTAVGTGLNT+ G
Sbjct: 229 IKIGRTHTQDAVPLTLGQEFSGYVQQVKYAVTRIKAAMPRIYELAAGGTAVGTGLNTRIG 288

Query: 273 FDVKIAAAVAEETNLPFVTAENKFEALAAHDAFVETSGSLNTVAASLMKIANDIRLLGSG 332
           F  K+AA VA  T LPFVTA NKFEALAAHDA VE SG++NT A SLMKIANDIR LGSG
Sbjct: 289 FAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSG 348

Query: 333 PRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAITVGGSNGHFELNVY 392
           PR GLGELILPENEPGSSIMPGKVNPTQCEA+TMV AQVMGNHVA+TVGGSNGHFELNV+
Sbjct: 349 PRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVF 408

Query: 393 KPMIANALLHSLRLLGDASASFEKNCVRGIQANRERISKLLHESLMLVTALNPKIGYDNA 452
           KPM+   +LHS RLLGDAS SF +NCV GIQAN ERI+KL++ESLMLVTALNP IGYD A
Sbjct: 409 KPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKA 468

Query: 453 AAVAKKAHKEGSTLKEAALKLGVLSEEDFDKHVVPEKMIGP 493
           A +AK AHK GSTLKE A++LG L+ E FD+ V P+ M+GP
Sbjct: 469 AKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGP 509

BLAST of CSPI04G01030 vs. ExPASy Swiss-Prot
Match: P07954 (Fumarate hydratase, mitochondrial OS=Homo sapiens OX=9606 GN=FH PE=1 SV=3)

HSP 1 Score: 667.9 bits (1722), Expect = 8.7e-191
Identity = 333/461 (72.23%), Postives = 381/461 (82.65%), Query Frame = 0

Query: 33  SFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIIRAFGVLKKCAAKVNM 92
           SFR E DTFG + VP+D+ +GAQT RS  NF+IGG  ERMP P+I+AFG+LK+ AA+VN 
Sbjct: 49  SFRIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILKRAAAEVNQ 108

Query: 93  DYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRASEILGHK 152
           DYGLDP I  AIM+AA EVAEGKLNDHFPLVVWQTGSGTQ+NMN NEVI+NRA E+LG +
Sbjct: 109 DYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNEVISNRAIEMLGGE 168

Query: 153 RGDKF-VHPNDHVNRSQSSNDTFPTVMHIAAATEINSKFIPKLKTLHSSLYSKSIEFKDI 212
            G K  VHPNDHVN+SQSSNDTFPT MHIAAA E++   +P L+ LH +L +KS EF  I
Sbjct: 169 LGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDALDAKSKEFAQI 228

Query: 213 VKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKG 272
           +KIGRTHTQDA PLTLGQEFSGY  QVKY + R+   +PR+Y+LA GGTAVGTGLNT+ G
Sbjct: 229 IKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIG 288

Query: 273 FDVKIAAAVAEETNLPFVTAENKFEALAAHDAFVETSGSLNTVAASLMKIANDIRLLGSG 332
           F  K+AA VA  T LPFVTA NKFEALAAHDA VE SG++NT A SLMKIANDIR LGSG
Sbjct: 289 FAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSG 348

Query: 333 PRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAITVGGSNGHFELNVY 392
           PR GLGELILPENEPGSSIMPGKVNPTQCEA+TMV AQVMGNHVA+TVGGSNGHFELNV+
Sbjct: 349 PRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVF 408

Query: 393 KPMIANALLHSLRLLGDASASFEKNCVRGIQANRERISKLLHESLMLVTALNPKIGYDNA 452
           KPM+   +LHS RLLGDAS SF +NCV GIQAN ERI+KL++ESLMLVTALNP IGYD A
Sbjct: 409 KPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKA 468

Query: 453 AAVAKKAHKEGSTLKEAALKLGVLSEEDFDKHVVPEKMIGP 493
           A +AK AHK GSTLKE A++LG L+ E FD+ V P+ M+GP
Sbjct: 469 AKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGP 509

BLAST of CSPI04G01030 vs. ExPASy TrEMBL
Match: A0A0A0KWM0 (Fumarate hydratase OS=Cucumis sativus OX=3659 GN=Csa_4G001990 PE=3 SV=1)

HSP 1 Score: 979.5 bits (2531), Expect = 5.0e-282
Identity = 495/495 (100.00%), Postives = 495/495 (100.00%), Query Frame = 0

Query: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300

Query: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDF 480
           IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDF
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDF 480

Query: 481 DKHVVPEKMIGPSDC 496
           DKHVVPEKMIGPSDC
Sbjct: 481 DKHVVPEKMIGPSDC 495

BLAST of CSPI04G01030 vs. ExPASy TrEMBL
Match: A0A5A7TQL4 (Fumarate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G001250 PE=3 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 4.8e-277
Identity = 486/493 (98.58%), Postives = 490/493 (99.39%), Query Frame = 0

Query: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAAVYVLSRRSS SHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNM+YGLDPT+GKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTVGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRG+KFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300

Query: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDF 480
           IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEG TLKEAALKLGVL EE+F
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGCTLKEAALKLGVLCEEEF 480

Query: 481 DKHVVPEKMIGPS 494
           DKHVVPEKMIGPS
Sbjct: 481 DKHVVPEKMIGPS 493

BLAST of CSPI04G01030 vs. ExPASy TrEMBL
Match: A0A5D3E117 (Fumarate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001270 PE=3 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 8.3e-277
Identity = 486/493 (98.58%), Postives = 490/493 (99.39%), Query Frame = 0

Query: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAAVYVLSRRSS SHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNM+YGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRG+KFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPF+TAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFMTAENKFEALAA 300

Query: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDF 480
           IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEG TLKEAALKLGVL EE+F
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGCTLKEAALKLGVLCEEEF 480

Query: 481 DKHVVPEKMIGPS 494
           DKHVVPEKMIGPS
Sbjct: 481 DKHVVPEKMIGPS 493

BLAST of CSPI04G01030 vs. ExPASy TrEMBL
Match: A0A1S3BXT7 (Fumarate hydratase OS=Cucumis melo OX=3656 GN=LOC103494751 PE=3 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 8.3e-277
Identity = 486/493 (98.58%), Postives = 490/493 (99.39%), Query Frame = 0

Query: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAAVYVLSRRSS SHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNM+YGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRG+KFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPF+TAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFMTAENKFEALAA 300

Query: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDF 480
           IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEG TLKEAALKLGVL EE+F
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGCTLKEAALKLGVLCEEEF 480

Query: 481 DKHVVPEKMIGPS 494
           DKHVVPEKMIGPS
Sbjct: 481 DKHVVPEKMIGPS 493

BLAST of CSPI04G01030 vs. ExPASy TrEMBL
Match: A0A6J1J9G0 (Fumarate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111482474 PE=3 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 1.6e-272
Identity = 480/494 (97.17%), Postives = 488/494 (98.79%), Query Frame = 0

Query: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA+YVLSRRSS SHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAALYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNM+YGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRG+KFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINSKFIPKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300
           GIDRVL TLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PF+TAENKFEALAA
Sbjct: 241 GIDRVLRTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFLTAENKFEALAA 300

Query: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSG+LNTVA SLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVAITVGGSNGHFELNV+KPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDF 480
           IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAK AHKEGSTLKEAALKLGVLSEEDF
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALKLGVLSEEDF 480

Query: 481 DKHVVPEKMIGPSD 495
           +K+VVPEKMIGPSD
Sbjct: 481 NKYVVPEKMIGPSD 494

BLAST of CSPI04G01030 vs. NCBI nr
Match: XP_004152237.1 (fumarate hydratase 1, mitochondrial [Cucumis sativus] >KGN52832.1 hypothetical protein Csa_014636 [Cucumis sativus])

HSP 1 Score: 979.5 bits (2531), Expect = 1.0e-281
Identity = 495/495 (100.00%), Postives = 495/495 (100.00%), Query Frame = 0

Query: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300

Query: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDF 480
           IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDF
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDF 480

Query: 481 DKHVVPEKMIGPSDC 496
           DKHVVPEKMIGPSDC
Sbjct: 481 DKHVVPEKMIGPSDC 495

BLAST of CSPI04G01030 vs. NCBI nr
Match: KAA0044396.1 (fumarate hydratase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 963.0 bits (2488), Expect = 1.0e-276
Identity = 486/493 (98.58%), Postives = 490/493 (99.39%), Query Frame = 0

Query: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAAVYVLSRRSS SHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNM+YGLDPT+GKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTVGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRG+KFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300

Query: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDF 480
           IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEG TLKEAALKLGVL EE+F
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGCTLKEAALKLGVLCEEEF 480

Query: 481 DKHVVPEKMIGPS 494
           DKHVVPEKMIGPS
Sbjct: 481 DKHVVPEKMIGPS 493

BLAST of CSPI04G01030 vs. NCBI nr
Match: XP_008454312.1 (PREDICTED: fumarate hydratase 1, mitochondrial [Cucumis melo] >TYK29524.1 fumarate hydratase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 962.2 bits (2486), Expect = 1.7e-276
Identity = 486/493 (98.58%), Postives = 490/493 (99.39%), Query Frame = 0

Query: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAAVYVLSRRSS SHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNM+YGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRG+KFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPF+TAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFMTAENKFEALAA 300

Query: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDF 480
           IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEG TLKEAALKLGVL EE+F
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGCTLKEAALKLGVLCEEEF 480

Query: 481 DKHVVPEKMIGPS 494
           DKHVVPEKMIGPS
Sbjct: 481 DKHVVPEKMIGPS 493

BLAST of CSPI04G01030 vs. NCBI nr
Match: XP_023528894.1 (fumarate hydratase 1, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 949.1 bits (2452), Expect = 1.5e-272
Identity = 481/494 (97.37%), Postives = 488/494 (98.79%), Query Frame = 0

Query: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAAVYVLSRRSS SHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNM+YGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRG+KFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINSKFIPKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300
           GIDRVL TLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPF+TAENKFEALAA
Sbjct: 241 GIDRVLRTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFLTAENKFEALAA 300

Query: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSG+LNTVA SLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVAITVGGSNGHFELNV+KPMIANALLHSLRLLGDAS SFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVFKPMIANALLHSLRLLGDASGSFEKNCVRG 420

Query: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDF 480
           IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAK AHKEGSTLKEAALKLGVLSEEDF
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALKLGVLSEEDF 480

Query: 481 DKHVVPEKMIGPSD 495
           +K+VVPEKMIGPSD
Sbjct: 481 NKYVVPEKMIGPSD 494

BLAST of CSPI04G01030 vs. NCBI nr
Match: XP_022984048.1 (fumarate hydratase 1, mitochondrial-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 948.0 bits (2449), Expect = 3.3e-272
Identity = 480/494 (97.17%), Postives = 488/494 (98.79%), Query Frame = 0

Query: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA+YVLSRRSS SHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAALYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNM+YGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRG+KFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINSKFIPKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300
           GIDRVL TLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PF+TAENKFEALAA
Sbjct: 241 GIDRVLRTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFLTAENKFEALAA 300

Query: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSG+LNTVA SLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVAITVGGSNGHFELNV+KPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDF 480
           IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAK AHKEGSTLKEAALKLGVLSEEDF
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALKLGVLSEEDF 480

Query: 481 DKHVVPEKMIGPSD 495
           +K+VVPEKMIGPSD
Sbjct: 481 NKYVVPEKMIGPSD 494

BLAST of CSPI04G01030 vs. TAIR 10
Match: AT2G47510.1 (fumarase 1 )

HSP 1 Score: 870.5 bits (2248), Expect = 6.3e-253
Identity = 437/492 (88.82%), Postives = 462/492 (93.90%), Query Frame = 0

Query: 3   AVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQN 62
           ++YV SRR S        RYA TSLRSYSTSFREERDTFGPI VPSD+LWGAQTQRSLQN
Sbjct: 2   SIYVASRRLSGGTTVTALRYA-TSLRSYSTSFREERDTFGPIQVPSDKLWGAQTQRSLQN 61

Query: 63  FEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHFPL 122
           FEIGG+RERMPEPI+RAFGVLKKCAAKVNM+YGLDPTIGKAIMQAAQEVAEGKLNDHFPL
Sbjct: 62  FEIGGERERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPL 121

Query: 123 VVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHIAA 182
           VVWQTGSGTQSNMNANEVIANRA+EILG KRG+K VHPNDHVNRSQSSNDTFPTVMHIAA
Sbjct: 122 VVWQTGSGTQSNMNANEVIANRAAEILGRKRGEKCVHPNDHVNRSQSSNDTFPTVMHIAA 181

Query: 183 ATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGI 242
           ATEINS+ IP LKTLHS+L SKS EFKDIVKIGRTHTQDATPLTLGQEF GY TQVKYG+
Sbjct: 182 ATEINSRLIPSLKTLHSTLESKSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVKYGL 241

Query: 243 DRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHD 302
           +RV CTLPR+YQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHD
Sbjct: 242 NRVTCTLPRLYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHD 301

Query: 303 AFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEA 362
           A VETSGSLNT+A SLMKIANDIR LGSGPRCGLGEL+LPENEPGSSIMPGKVNPTQCEA
Sbjct: 302 ACVETSGSLNTIATSLMKIANDIRFLGSGPRCGLGELVLPENEPGSSIMPGKVNPTQCEA 361

Query: 363 LTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRGIQ 422
           LTMVCAQVMGNHVA+TVGGSNGHFELNV+KP+IA+ALLHS+RL+ DASASFEKNCVRGI+
Sbjct: 362 LTMVCAQVMGNHVAVTVGGSNGHFELNVFKPVIASALLHSVRLIADASASFEKNCVRGIE 421

Query: 423 ANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDFDK 482
           ANRERISKLLHESLMLVT+LNPKIGYDNAAAVAKKAHKEG TLKEAAL LGVL+ E+FD 
Sbjct: 422 ANRERISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGCTLKEAALNLGVLTAEEFDT 481

Query: 483 HVVPEKMIGPSD 495
            VVPEKMIGPSD
Sbjct: 482 LVVPEKMIGPSD 492

BLAST of CSPI04G01030 vs. TAIR 10
Match: AT2G47510.2 (fumarase 1 )

HSP 1 Score: 870.5 bits (2248), Expect = 6.3e-253
Identity = 437/492 (88.82%), Postives = 462/492 (93.90%), Query Frame = 0

Query: 3   AVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQN 62
           ++YV SRR S        RYA TSLRSYSTSFREERDTFGPI VPSD+LWGAQTQRSLQN
Sbjct: 2   SIYVASRRLSGGTTVTALRYA-TSLRSYSTSFREERDTFGPIQVPSDKLWGAQTQRSLQN 61

Query: 63  FEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHFPL 122
           FEIGG+RERMPEPI+RAFGVLKKCAAKVNM+YGLDPTIGKAIMQAAQEVAEGKLNDHFPL
Sbjct: 62  FEIGGERERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPL 121

Query: 123 VVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHIAA 182
           VVWQTGSGTQSNMNANEVIANRA+EILG KRG+K VHPNDHVNRSQSSNDTFPTVMHIAA
Sbjct: 122 VVWQTGSGTQSNMNANEVIANRAAEILGRKRGEKCVHPNDHVNRSQSSNDTFPTVMHIAA 181

Query: 183 ATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGI 242
           ATEINS+ IP LKTLHS+L SKS EFKDIVKIGRTHTQDATPLTLGQEF GY TQVKYG+
Sbjct: 182 ATEINSRLIPSLKTLHSTLESKSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVKYGL 241

Query: 243 DRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHD 302
           +RV CTLPR+YQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHD
Sbjct: 242 NRVTCTLPRLYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHD 301

Query: 303 AFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEA 362
           A VETSGSLNT+A SLMKIANDIR LGSGPRCGLGEL+LPENEPGSSIMPGKVNPTQCEA
Sbjct: 302 ACVETSGSLNTIATSLMKIANDIRFLGSGPRCGLGELVLPENEPGSSIMPGKVNPTQCEA 361

Query: 363 LTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRGIQ 422
           LTMVCAQVMGNHVA+TVGGSNGHFELNV+KP+IA+ALLHS+RL+ DASASFEKNCVRGI+
Sbjct: 362 LTMVCAQVMGNHVAVTVGGSNGHFELNVFKPVIASALLHSVRLIADASASFEKNCVRGIE 421

Query: 423 ANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDFDK 482
           ANRERISKLLHESLMLVT+LNPKIGYDNAAAVAKKAHKEG TLKEAAL LGVL+ E+FD 
Sbjct: 422 ANRERISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGCTLKEAALNLGVLTAEEFDT 481

Query: 483 HVVPEKMIGPSD 495
            VVPEKMIGPSD
Sbjct: 482 LVVPEKMIGPSD 492

BLAST of CSPI04G01030 vs. TAIR 10
Match: AT5G50950.2 (FUMARASE 2 )

HSP 1 Score: 847.8 bits (2189), Expect = 4.4e-246
Identity = 418/467 (89.51%), Postives = 444/467 (95.07%), Query Frame = 0

Query: 28  RSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIIRAFGVLKKCA 87
           RSYST FREERDTFGPI VPSD+LWGAQTQRSLQNFEIGGDRERMPEPI+RAFGVLKKCA
Sbjct: 33  RSYSTPFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 92

Query: 88  AKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRASE 147
           AKVNM+YGLDP IG+AIM+AAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRA+E
Sbjct: 93  AKVNMEYGLDPMIGEAIMEAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRAAE 152

Query: 148 ILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSKFIPKLKTLHSSLYSKSIE 207
           ILGHKRG+K VHPNDHVNRSQSSNDTFPTVMHIAAATEI S+ IP LK LHSSL SKS E
Sbjct: 153 ILGHKRGEKIVHPNDHVNRSQSSNDTFPTVMHIAAATEITSRLIPSLKNLHSSLESKSFE 212

Query: 208 FKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLN 267
           FKDIVKIGRTHTQDATPLTLGQEF GY TQV+YG++RV CTLPR+YQLAQGGTAVGTGLN
Sbjct: 213 FKDIVKIGRTHTQDATPLTLGQEFGGYATQVEYGLNRVACTLPRIYQLAQGGTAVGTGLN 272

Query: 268 TKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDAFVETSGSLNTVAASLMKIANDIRL 327
           TKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDA VETSGSLNT+A SLMKIANDIR 
Sbjct: 273 TKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDACVETSGSLNTIATSLMKIANDIRF 332

Query: 328 LGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAITVGGSNGHFE 387
           LGSGPRCGLGEL LPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVA+T+GGSNGHFE
Sbjct: 333 LGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTIGGSNGHFE 392

Query: 388 LNVYKPMIANALLHSLRLLGDASASFEKNCVRGIQANRERISKLLHESLMLVTALNPKIG 447
           LNV+KP+IA+ALLHS+RL+ DASASFEKNCVRGI+ANRERISKLLHESLMLVT+LNPKIG
Sbjct: 393 LNVFKPVIASALLHSIRLIADASASFEKNCVRGIEANRERISKLLHESLMLVTSLNPKIG 452

Query: 448 YDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDFDKHVVPEKMIGPSD 495
           YDNAAAVAK+AHKEG TLK AA+KLGVL+ E+FD  VVPEKMIGPSD
Sbjct: 453 YDNAAAVAKRAHKEGCTLKHAAMKLGVLTSEEFDTLVVPEKMIGPSD 499

BLAST of CSPI04G01030 vs. TAIR 10
Match: AT5G50950.1 (FUMARASE 2 )

HSP 1 Score: 806.6 bits (2082), Expect = 1.1e-233
Identity = 397/439 (90.43%), Postives = 420/439 (95.67%), Query Frame = 0

Query: 28  RSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIIRAFGVLKKCA 87
           RSYST FREERDTFGPI VPSD+LWGAQTQRSLQNFEIGGDRERMPEPI+RAFGVLKKCA
Sbjct: 33  RSYSTPFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 92

Query: 88  AKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRASE 147
           AKVNM+YGLDP IG+AIM+AAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRA+E
Sbjct: 93  AKVNMEYGLDPMIGEAIMEAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRAAE 152

Query: 148 ILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSKFIPKLKTLHSSLYSKSIE 207
           ILGHKRG+K VHPNDHVNRSQSSNDTFPTVMHIAAATEI S+ IP LK LHSSL SKS E
Sbjct: 153 ILGHKRGEKIVHPNDHVNRSQSSNDTFPTVMHIAAATEITSRLIPSLKNLHSSLESKSFE 212

Query: 208 FKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLN 267
           FKDIVKIGRTHTQDATPLTLGQEF GY TQV+YG++RV CTLPR+YQLAQGGTAVGTGLN
Sbjct: 213 FKDIVKIGRTHTQDATPLTLGQEFGGYATQVEYGLNRVACTLPRIYQLAQGGTAVGTGLN 272

Query: 268 TKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDAFVETSGSLNTVAASLMKIANDIRL 327
           TKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDA VETSGSLNT+A SLMKIANDIR 
Sbjct: 273 TKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDACVETSGSLNTIATSLMKIANDIRF 332

Query: 328 LGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAITVGGSNGHFE 387
           LGSGPRCGLGEL LPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVA+T+GGSNGHFE
Sbjct: 333 LGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTIGGSNGHFE 392

Query: 388 LNVYKPMIANALLHSLRLLGDASASFEKNCVRGIQANRERISKLLHESLMLVTALNPKIG 447
           LNV+KP+IA+ALLHS+RL+ DASASFEKNCVRGI+ANRERISKLLHESLMLVT+LNPKIG
Sbjct: 393 LNVFKPVIASALLHSIRLIADASASFEKNCVRGIEANRERISKLLHESLMLVTSLNPKIG 452

Query: 448 YDNAAAVAKKAHKEGSTLK 467
           YDNAAAVAK+AHKEG TLK
Sbjct: 453 YDNAAAVAKRAHKEGCTLK 471

BLAST of CSPI04G01030 vs. TAIR 10
Match: AT5G50950.3 (FUMARASE 2 )

HSP 1 Score: 763.1 bits (1969), Expect = 1.4e-220
Identity = 377/423 (89.13%), Postives = 402/423 (95.04%), Query Frame = 0

Query: 72  MPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGT 131
           MPEPI+RAFGVLKKCAAKVNM+YGLDP IG+AIM+AAQEVAEGKLNDHFPLVVWQTGSGT
Sbjct: 1   MPEPIVRAFGVLKKCAAKVNMEYGLDPMIGEAIMEAAQEVAEGKLNDHFPLVVWQTGSGT 60

Query: 132 QSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSKFI 191
           QSNMNANEVIANRA+EILGHKRG+K VHPNDHVNRSQSSNDTFPTVMHIAAATEI S+ I
Sbjct: 61  QSNMNANEVIANRAAEILGHKRGEKIVHPNDHVNRSQSSNDTFPTVMHIAAATEITSRLI 120

Query: 192 PKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPR 251
           P LK LHSSL SKS EFKDIVKIGRTHTQDATPLTLGQEF GY TQV+YG++RV CTLPR
Sbjct: 121 PSLKNLHSSLESKSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVEYGLNRVACTLPR 180

Query: 252 MYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDAFVETSGSL 311
           +YQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDA VETSGSL
Sbjct: 181 IYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDACVETSGSL 240

Query: 312 NTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVM 371
           NT+A SLMKIANDIR LGSGPRCGLGEL LPENEPGSSIMPGKVNPTQCEALTMVCAQVM
Sbjct: 241 NTIATSLMKIANDIRFLGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEALTMVCAQVM 300

Query: 372 GNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRGIQANRERISKL 431
           GNHVA+T+GGSNGHFELNV+KP+IA+ALLHS+RL+ DASASFEKNCVRGI+ANRERISKL
Sbjct: 301 GNHVAVTIGGSNGHFELNVFKPVIASALLHSIRLIADASASFEKNCVRGIEANRERISKL 360

Query: 432 LHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDFDKHVVPEKMIG 491
           LHESLMLVT+LNPKIGYDNAAAVAK+AHKEG TLK AA+KLGVL+ E+FD  VVPEKMIG
Sbjct: 361 LHESLMLVTSLNPKIGYDNAAAVAKRAHKEGCTLKHAAMKLGVLTSEEFDTLVVPEKMIG 420

Query: 492 PSD 495
           PSD
Sbjct: 421 PSD 423

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P930338.9e-25288.82Fumarate hydratase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FUM1 PE=1... [more]
Q9FI536.1e-24589.51Fumarate hydratase 2 OS=Arabidopsis thaliana OX=3702 GN=FUM2 PE=1 SV=1[more]
P552502.1e-19269.51Fumarate hydratase, mitochondrial OS=Rhizopus oryzae OX=64495 GN=FUMR PE=3 SV=1[more]
Q60HF95.1e-19172.23Fumarate hydratase, mitochondrial OS=Macaca fascicularis OX=9541 GN=FH PE=2 SV=1[more]
P079548.7e-19172.23Fumarate hydratase, mitochondrial OS=Homo sapiens OX=9606 GN=FH PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A0A0KWM05.0e-282100.00Fumarate hydratase OS=Cucumis sativus OX=3659 GN=Csa_4G001990 PE=3 SV=1[more]
A0A5A7TQL44.8e-27798.58Fumarate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00... [more]
A0A5D3E1178.3e-27798.58Fumarate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G0... [more]
A0A1S3BXT78.3e-27798.58Fumarate hydratase OS=Cucumis melo OX=3656 GN=LOC103494751 PE=3 SV=1[more]
A0A6J1J9G01.6e-27297.17Fumarate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111482474 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004152237.11.0e-281100.00fumarate hydratase 1, mitochondrial [Cucumis sativus] >KGN52832.1 hypothetical p... [more]
KAA0044396.11.0e-27698.58fumarate hydratase 1 [Cucumis melo var. makuwa][more]
XP_008454312.11.7e-27698.58PREDICTED: fumarate hydratase 1, mitochondrial [Cucumis melo] >TYK29524.1 fumara... [more]
XP_023528894.11.5e-27297.37fumarate hydratase 1, mitochondrial-like [Cucurbita pepo subsp. pepo][more]
XP_022984048.13.3e-27297.17fumarate hydratase 1, mitochondrial-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G47510.16.3e-25388.82fumarase 1 [more]
AT2G47510.26.3e-25388.82fumarase 1 [more]
AT5G50950.24.4e-24689.51FUMARASE 2 [more]
AT5G50950.11.1e-23390.43FUMARASE 2 [more]
AT5G50950.31.4e-22089.13FUMARASE 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000362Fumarate lyase familyPRINTSPR00149FUMRATELYASEcoord: 347..363
score: 66.75
coord: 301..328
score: 36.35
coord: 164..182
score: 38.81
coord: 210..228
score: 55.26
IPR018951Fumarase C, C-terminalPFAMPF10415FumaraseC_Ccoord: 438..491
e-value: 1.1E-24
score: 86.6
NoneNo IPR availableGENE3D1.10.40.30coord: 437..494
e-value: 6.2E-28
score: 98.8
NoneNo IPR availableGENE3D1.20.200.10Fumarase/aspartase (Central domain)coord: 170..436
e-value: 2.3E-105
score: 353.7
NoneNo IPR availablePANTHERPTHR11444:SF24FUMARATE HYDRATASE, MITOCHONDRIALcoord: 16..494
IPR005677Fumarate hydratase, class IITIGRFAMTIGR00979TIGR00979coord: 34..491
e-value: 6.6E-230
score: 761.5
IPR005677Fumarate hydratase, class IIPANTHERPTHR11444ASPARTATEAMMONIA/ARGININOSUCCINATE/ADENYLOSUCCINATE LYASEcoord: 16..494
IPR005677Fumarate hydratase, class IIHAMAPMF_00743FumaraseCcoord: 34..492
score: 88.538872
IPR005677Fumarate hydratase, class IICDDcd01362Fumarase_classIIcoord: 35..489
e-value: 0.0
score: 872.593
IPR024083Fumarase/histidase, N-terminalGENE3D1.10.275.10coord: 35..169
e-value: 2.3E-63
score: 214.5
IPR022761Fumarate lyase, N-terminalPFAMPF00206Lyase_1coord: 42..372
e-value: 7.0E-116
score: 387.0
IPR020557Fumarate lyase, conserved sitePROSITEPS00163FUMARATE_LYASEScoord: 347..356
IPR008948L-Aspartase-likeSUPERFAMILY48557L-aspartase-likecoord: 34..490

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G01030.1CSPI04G01030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006106 fumarate metabolic process
biological_process GO:0006108 malate metabolic process
biological_process GO:0006099 tricarboxylic acid cycle
cellular_component GO:0005739 mitochondrion
cellular_component GO:0045239 tricarboxylic acid cycle enzyme complex
molecular_function GO:0004333 fumarate hydratase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016829 lyase activity