CSPI04G00220 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G00220
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Adenylyl-sulfate kinase
Location	Chr4: 159590 .. 162568 (-)
RNA-Seq Expression	CSPI04G00220
Synteny	CSPI04G00220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGACAACTCGCACCCATTTTAATGCGTTGGCTTTCGTCGGACTTTAAACGGAGTCGTTTTTCACTTCTTCTTCTTCTTCTCCGGACGGCTCGCTGTTCCGCAGGCTTCGGCTCCGGCTCCGCATTCTTCTTCTACGGTACATAAATTTCAGGGAAGAAGAAGAATCTAAGTGAAAGGTTCTAAACAAGGACTCATCGCGAAAAGGGCTCGTGATTCTTGTTTTTCTTCTTCTTCTTATTTTTTTTTCTCTCTTTTTTTTTTTTTTTGTTTTCCACCGGTCATTAATTATTATGGCGGACAAGAACGTGAGAGGCTCTCCGCCATTCGACCAATTATGGCATCTCATTTCCTCTTGATTTCATCTCTCCATTTCTTTTTTAGTTTTTTCTATTTATTTTTCAGTAAATGGTGTTTTCCTTTTGAGATGTGTGTGAGATATGGTTTCGATTGGAGGAATTTCGTTGCCGGGTTCTATACCGCCGTCGCTTCACCACCAATCATCTCCGACGTCGGCGATTGGGTTCGCTAGGTTTCCCGACAGGATTAGCTTTAGATCGTCGGTGGTTTTGCGGGATGAAGTTGAGAAGAAGGGTTCGAGGGTGGTGATTGTTAATGGGAAGGTCGATGGTTTGGGTAAGAGTGAATGTGAGACTGACTTTGATACTGGCTTGGGCAATGGACGTGCTGGGAACTTGGGTATTGCTTCAATTTTGATGTTTCTTGATGATATAATCTTGAATCTTGATTCGGGGACTTTAATTTTGGTTGAATCGGAATATCTGTTATTTTTAGTTCTTGAATTTTTTAATGTATAGTGAGATATGGGAACTTCTCTTTGAATCATCTCATTGAAATTTCTGGTGTTTTGATCGTCTTGTTTGATTCATTAGATAGTGGAAATAAGATAATTGGAAAAGCAAGAACCTTATTGTTAGTATTTATGTAGATATTAGTTATATTTTCATTGACATTTTCAGTATTTTTCTACACATTTACAATGTGATGTTGAACGCTTTGATTTGTTGGAATGCTGATTTTATTGCACAATGTTTGAATTGTTGCCATGTTGGCATAAATTTATAATGTGATATCCAATTTTTCTTCATCATGGTATGAGTTTATCTTCTGTAGTTTAAGCTGTTCAGATTACTAAGATGCTTTTCAATCAGGAAAAAATCATGGAGTGTTATCCACGGTAGGAAATTCTACTAATATCAAGTGGCATGAATGTTCAGTTGGAAAGAATGAAAAACAGAGCCTTCTCAAACAGAAAGGATGCGTGATATGGTTCACAGGTCTCAGTGGCTCAGGTTTGTCATTGAACCTTTTGTCTTTTGCTTTTACAGCGTAATGGTTTTTGACTTATTATGTAATAATCATGGTTTAGAGAACCCATAGTTTAGATTGTCCTCAAATGAAACCTACAGTTTGGATTGTAGACCAAACCCAACAATGGAACATTTGATTTTGATTTTTGAGGTCATTCTTTTAAAAGATTTCGTGAAAAGGATATAAAAGAATTTGGCCTGAAATATTTAAGTTGGGTTTGGGACTTTATAGACTGGCCCAATTTTTTTAAGTATGCTTAGCTAATTTGCAGGGAAGAGCACTGTGGCCTGTGCCTTGAGTCAAAGCTTATACAAAATGGGGAAGTTAGCTTATATCCTTGATGGGGACAATGTGAGGCATGGCTTGAATCGTGACCTTGGTTTTAAAGCAGAAGATCGTGCTGAGAATATAAGGAGGGTTGGTAAGTTTGGTTCATTATCATTTATAATAACTCTCTTTGCTGTTCTGTTAAGGATTGCTAATTAGTATACCTCTTCCATAAATTAGGTGAGGTTGCAAAACTGTTTGCAGACGCTGGAGTTATTTGCATTGCTAGTTTGATATCCCCATATCGAAGGGAACGAGATGCCTGTCGTGACATTTTGCCTAATGGATACTTTATTGAGGTATCTCTTCATTTTGCGTACTATAGATTTTGAGCTGGTTTCTTACCTTGCATCTGAAATATATATTACAAAAATTTGCTAAAAATATATAGGTGTTCATGGATGTTCCTCTTGAAGTTTGTGAAGCAAGAGATGCCAAAGGACTGTATAAGCTTGCACGGGCAGGGAAGATCAAAGGTCACTGCAACTTGTTATCATTTTCCTTAGATTTACTTGATGATCTTATTAATTCTCACTTATTTTGTAGTGTGATTTGAGTATTACTTTTAACCTTATTTTGGCCCCTTATTTTCACAACCTAAGTTTCAACTAAAAGACATAATCAAATAAAATTAAGTTAATTATGCTCAGAATTATTAATTTTCATTTATCAACTAAGCAATATCACTTTTTTGCAGGCTTTACCGGTATCGATGATCCGTACGAAGTACCATTTAATTGTGAGGTAATTCCAAATCCTGAAGGAAATGATAACATCTCTTTAAAAGATTAATGCTTATACTACAGAGTGTAAATCTTTTTGCAACATATGCTGATAGTTAGTTAAATACCGAGAAGAACTTTACGAAAATAACTGGTTGTTTTATGAATGAGACATTGTGTATTGAATCTGATTGGGAGACCCTTTTGAACTTTTTGGGTTTCAGATAGTCTTGAAACACACTGGAGGTTCACCATCTGAGATGGCAGAGAAAGTAATGAGTTACCTAGAGCAGAAAGGCTTCCTCCAGGCTTAGCTCAAAGCTTTATGAGAAAAGGGGGCTTTTAACAACCTAAGTTTTGGTTTTAGAGAAAATCCACTTAACTAAAGCTTTGTCTAGGCTTATTTGTCTGTCACATCTAATTTGCTTCGTCTTTGGTCACTTCTTGTTCTGCATACAGATAGGATTGAAGGTTTGTGAGGATACATAATTTTCTTTTGCTTTTTTCCCCCTTTTGATCTCTGTAATGTAATATAGGTTTGAATGTTTTATACAAAAAAGTATATCAAATATTTTTGTGGAAATCAAGAAGTCAATCACAA

mRNA sequence

AGACAACTCGCACCCATTTTAATGCGTTGGCTTTCGTCGGACTTTAAACGGAGTCGTTTTTCACTTCTTCTTCTTCTTCTCCGGACGGCTCGCTGTTCCGCAGGCTTCGGCTCCGGCTCCGCATTCTTCTTCTACGGTACATAAATTTCAGGGAAGAAGAAGAATCTAAGTGAAAGGTTCTAAACAAGGACTCATCGCGAAAAGGGCTCGTGATTCTTGTTTTTCTTCTTCTTCTTATTTTTTTTTCTCTCTTTTTTTTTTTTTTTGTTTTCCACCGGTCATTAATTATTATGGCGGACAAGAACGTGAGAGGCTCTCCGCCATTCGACCAATTATGGCATCTCATTTCCTCTTGATTTCATCTCTCCATTTCTTTTTTAGTTTTTTCTATTTATTTTTCAGTAAATGGTGTTTTCCTTTTGAGATGTGTGTGAGATATGGTTTCGATTGGAGGAATTTCGTTGCCGGGTTCTATACCGCCGTCGCTTCACCACCAATCATCTCCGACGTCGGCGATTGGGTTCGCTAGGTTTCCCGACAGGATTAGCTTTAGATCGTCGGTGGTTTTGCGGGATGAAGTTGAGAAGAAGGGTTCGAGGGTGGTGATTGTTAATGGGAAGGTCGATGGTTTGGGTAAGAGTGAATGTGAGACTGACTTTGATACTGGCTTGGGCAATGGACGTGCTGGGAACTTGGGAAAAAATCATGGAGTGTTATCCACGGTAGGAAATTCTACTAATATCAAGTGGCATGAATGTTCAGTTGGAAAGAATGAAAAACAGAGCCTTCTCAAACAGAAAGGATGCGTGATATGGTTCACAGGTCTCAGTGGCTCAGGGAAGAGCACTGTGGCCTGTGCCTTGAGTCAAAGCTTATACAAAATGGGGAAGTTAGCTTATATCCTTGATGGGGACAATGTGAGGCATGGCTTGAATCGTGACCTTGGTTTTAAAGCAGAAGATCGTGCTGAGAATATAAGGAGGGTTGGTGAGGTTGCAAAACTGTTTGCAGACGCTGGAGTTATTTGCATTGCTAGTTTGATATCCCCATATCGAAGGGAACGAGATGCCTGTCGTGACATTTTGCCTAATGGATACTTTATTGAGGTGTTCATGGATGTTCCTCTTGAAGTTTGTGAAGCAAGAGATGCCAAAGGACTGTATAAGCTTGCACGGGCAGGGAAGATCAAAGGCTTTACCGGTATCGATGATCCGTACGAAGTACCATTTAATTGTGAGATAGTCTTGAAACACACTGGAGGTTCACCATCTGAGATGGCAGAGAAAGTAATGAGTTACCTAGAGCAGAAAGGCTTCCTCCAGGCTTAGCTCAAAGCTTTATGAGAAAAGGGGGCTTTTAACAACCTAAGTTTTGGTTTTAGAGAAAATCCACTTAACTAAAGCTTTGTCTAGGCTTATTTGTCTGTCACATCTAATTTGCTTCGTCTTTGGTCACTTCTTGTTCTGCATACAGATAGGATTGAAGGTTTGTGAGGATACATAATTTTCTTTTGCTTTTTTCCCCCTTTTGATCTCTGTAATGTAATATAGGTTTGAATGTTTTATACAAAAAAGTATATCAAATATTTTTGTGGAAATCAAGAAGTCAATCACAA

Coding sequence (CDS)

ATGGTTTCGATTGGAGGAATTTCGTTGCCGGGTTCTATACCGCCGTCGCTTCACCACCAATCATCTCCGACGTCGGCGATTGGGTTCGCTAGGTTTCCCGACAGGATTAGCTTTAGATCGTCGGTGGTTTTGCGGGATGAAGTTGAGAAGAAGGGTTCGAGGGTGGTGATTGTTAATGGGAAGGTCGATGGTTTGGGTAAGAGTGAATGTGAGACTGACTTTGATACTGGCTTGGGCAATGGACGTGCTGGGAACTTGGGAAAAAATCATGGAGTGTTATCCACGGTAGGAAATTCTACTAATATCAAGTGGCATGAATGTTCAGTTGGAAAGAATGAAAAACAGAGCCTTCTCAAACAGAAAGGATGCGTGATATGGTTCACAGGTCTCAGTGGCTCAGGGAAGAGCACTGTGGCCTGTGCCTTGAGTCAAAGCTTATACAAAATGGGGAAGTTAGCTTATATCCTTGATGGGGACAATGTGAGGCATGGCTTGAATCGTGACCTTGGTTTTAAAGCAGAAGATCGTGCTGAGAATATAAGGAGGGTTGGTGAGGTTGCAAAACTGTTTGCAGACGCTGGAGTTATTTGCATTGCTAGTTTGATATCCCCATATCGAAGGGAACGAGATGCCTGTCGTGACATTTTGCCTAATGGATACTTTATTGAGGTGTTCATGGATGTTCCTCTTGAAGTTTGTGAAGCAAGAGATGCCAAAGGACTGTATAAGCTTGCACGGGCAGGGAAGATCAAAGGCTTTACCGGTATCGATGATCCGTACGAAGTACCATTTAATTGTGAGATAGTCTTGAAACACACTGGAGGTTCACCATCTGAGATGGCAGAGAAAGTAATGAGTTACCTAGAGCAGAAAGGCTTCCTCCAGGCTTAG

Protein sequence

MVSIGGISLPGSIPPSLHHQSSPTSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNGKVDGLGKSECETDFDTGLGNGRAGNLGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQKGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGGSPSEMAEKVMSYLEQKGFLQA*

Homology

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BLAST of CSPI04G00220 vs. ExPASy Swiss-Prot
Match: Q43295 (Adenylyl-sulfate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK1 PE=1 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 2.2e-96
Identity = 171/214 (79.91%), Postives = 185/214 (86.45%), Query Frame = 0


Query: 84  GNLGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQKGCVIWFTGLSGSGKSTVACALS 143
           G+ G+  G LSTVGNSTNIKWHECSV K ++Q LL QKGCVIW TGLSGSGKST+ACAL+
Sbjct: 63  GHTGQKQGPLSTVGNSTNIKWHECSVEKVDRQRLLDQKGCVIWVTGLSGSGKSTLACALN 122

Query: 144 QSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLIS 203
           Q LY+ GKL YILDGDNVRHGLNRDL FKAEDRAENIRRVGEVAKLFADAG+ICIASLIS
Sbjct: 123 QMLYQKGKLCYILDGDNVRHGLNRDLSFKAEDRAENIRRVGEVAKLFADAGIICIASLIS 182

Query: 204 PYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVP 263
           PYR +RDACR +LP G F+EVFMDVPL VCEARD KGLYKLARAGKIKGFTGIDDPYE P
Sbjct: 183 PYRTDRDACRSLLPEGDFVEVFMDVPLSVCEARDPKGLYKLARAGKIKGFTGIDDPYEPP 242

Query: 264 FNCEIVLKHTGG-SPSEMAEKVMSYLEQKGFLQA 297
            NCEI L   GG SP EMAEKV+ YL+ KG+LQA
Sbjct: 243 LNCEISLGREGGTSPIEMAEKVVGYLDNKGYLQA 276

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BLAST of CSPI04G00220 vs. ExPASy Swiss-Prot
Match: O49204 (Adenylyl-sulfate kinase, chloroplastic OS=Catharanthus roseus OX=4058 GN=AKN PE=2 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 2.0e-89
Identity = 169/245 (68.98%), Postives = 194/245 (79.18%), Query Frame = 0


Query: 58  VNGKVDG---LGKSECETDFDTGLGNGRAGNLGKNHGVLSTVGNSTNIKWHECSVGKNEK 117
           VNGKVD    L  S+C ++ D+ L N   G  GK     +TVGNSTNI WH+C+V K+E+
Sbjct: 69  VNGKVDNFRHLQPSDCNSNSDSSLNNCN-GFPGKKILQTTTVGNSTNILWHKCAVEKSER 128

Query: 118 QSLLKQKGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAE 177
           Q  L+Q+GCVIW TGLSGSGKST+ACALS+ L+  GKL YILDGDNVRHGLN DL FKAE
Sbjct: 129 QEPLQQRGCVIWITGLSGSGKSTLACALSRGLHAKGKLTYILDGDNVRHGLNSDLSFKAE 188

Query: 178 DRAENIRRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCE 237
           DRAENIRR+GEVAKLFADAGVICIASLISPYR+  DACR +LP G FIEVFMDVPL+VCE
Sbjct: 189 DRAENIRRIGEVAKLFADAGVICIASLISPYRKPPDACRSLLPEGDFIEVFMDVPLKVCE 248

Query: 238 ARDAKGLYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGG---SPSEMAEKVMSYLEQK 297
           ARD KGLYKLARAGKIKGFTGIDDPYE P   EIVL    G   SP ++A+ V+SYLE+ 
Sbjct: 249 ARDPKGLYKLARAGKIKGFTGIDDPYEPPLKSEIVLHQKLGMCDSPCDLADIVISYLEEN 308

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BLAST of CSPI04G00220 vs. ExPASy Swiss-Prot
Match: Q9SRW7 (Adenylyl-sulfate kinase 3 OS=Arabidopsis thaliana OX=3702 GN=APK3 PE=1 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 3.2e-87
Identity = 154/206 (74.76%), Postives = 176/206 (85.44%), Query Frame = 0


Query: 93  LSTVGNSTNIKWHECSVGKNEKQSLLKQKGCVIWFTGLSGSGKSTVACALSQSLYKMGKL 152
           +STVGNSTNI W E  +GK E+Q LL QKGCV+W TGLSGSGKST+AC+LS+ L   GKL
Sbjct: 1   MSTVGNSTNIFWQESPIGKTERQKLLNQKGCVVWITGLSGSGKSTLACSLSRELNNRGKL 60

Query: 153 AYILDGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRERDAC 212
           +YILDGDN+RHGLN+DLGFKAEDR ENIRRVGEVAKLFADAG+ICIASLISPYR++RDAC
Sbjct: 61  SYILDGDNLRHGLNKDLGFKAEDRVENIRRVGEVAKLFADAGLICIASLISPYRKDRDAC 120

Query: 213 RDILPNGYFIEVFMDVPLEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVPFNCEIVLKH 272
           R+++ N  FIEVFM++ L++CEARD KGLYKLARAGKIKGFTGIDDPYE P NCEI LK 
Sbjct: 121 REMIQNSSFIEVFMNMSLQLCEARDPKGLYKLARAGKIKGFTGIDDPYESPLNCEIELKE 180

Query: 273 TGG---SPSEMAEKVMSYLEQKGFLQ 296
             G   SP  MAE+V+SYLE KGFLQ
Sbjct: 181 KEGECPSPVAMAEEVISYLEDKGFLQ 206

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BLAST of CSPI04G00220 vs. ExPASy Swiss-Prot
Match: O49196 (Adenylyl-sulfate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK2 PE=1 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 1.6e-86
Identity = 152/213 (71.36%), Postives = 178/213 (83.57%), Query Frame = 0


Query: 87  GKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQKGCVIWFTGLSGSGKSTVACALSQSL 146
           G+N+G       + NI WHE S+ + ++Q LL+QKGCV+W TGLSGSGKSTVACALS++L
Sbjct: 84  GRNNG----KEKAENIVWHESSICRCDRQQLLQQKGCVVWITGLSGSGKSTVACALSKAL 143

Query: 147 YKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYR 206
           ++ GKL Y LDGDNVRHGLNRDL FKAE R ENIRR+GEVAKLFAD GVICIASLISPYR
Sbjct: 144 FERGKLTYTLDGDNVRHGLNRDLTFKAEHRTENIRRIGEVAKLFADVGVICIASLISPYR 203

Query: 207 RERDACRDILPNGYFIEVFMDVPLEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVPFNC 266
           R+RDACR +LP+G F+EVFMDVPL VCE+RD KGLYKLARAGKIKGFTGIDDPYE P NC
Sbjct: 204 RDRDACRSLLPDGDFVEVFMDVPLHVCESRDPKGLYKLARAGKIKGFTGIDDPYEAPVNC 263

Query: 267 EIVLKHTGG----SPSEMAEKVMSYLEQKGFLQ 296
           E+VLKHTG     SP +MAE ++SYL+ KG+L+
Sbjct: 264 EVVLKHTGDDESCSPRQMAENIISYLQNKGYLE 292

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BLAST of CSPI04G00220 vs. ExPASy Swiss-Prot
Match: Q84JF0 (Adenylyl-sulfate kinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK4 PE=1 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 9.8e-81
Identity = 146/217 (67.28%), Postives = 173/217 (79.72%), Query Frame = 0


Query: 97  GNSTNIKWHECSVGKNEKQSLLKQKGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYIL 156
           G   NI WH+C V K+++Q L+KQKGCVIW TGLSGSGKS++ACALS++L+  GKL+YIL
Sbjct: 84  GKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGSGKSSLACALSRALHNRGKLSYIL 143

Query: 157 DGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRERDACRDIL 216
           DGDNVRHGLN DL F+A+DRAENIRRVGEVAKLFAD+G+ICIASLISPYR ER ACR +L
Sbjct: 144 DGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICIASLISPYRIERAACRALL 203

Query: 217 PNGYFIEVFMDVPLEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVPFNCEIVLKH---- 276
           P G FIEVFMDVPL VCEARD KGLYK ARAGKIKGFTG+DDPYE P +CEIV+++    
Sbjct: 204 PQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDDPYEAPLDCEIVIQNSRDK 263

Query: 277 -------TGGSPS-------EMAEKVMSYLEQKGFLQ 296
                  +  SPS       EMA+ V+SYL+Q G+L+
Sbjct: 264 GLSSSSSSSSSPSSSSSSLCEMADIVVSYLDQNGYLK 300

[Close]

BLAST of CSPI04G00220 vs. ExPASy TrEMBL
Match: A0A0A0KY24 (Adenylyl-sulfate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G000720 PE=3 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 1.8e-165
Identity = 291/296 (98.31%), Postives = 292/296 (98.65%), Query Frame = 0


Query: 1   MVSIGGISLPGSIPPSLHHQSSPTSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60
           MVSIGGISLPGSIPP LHHQSSPTSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVI NG
Sbjct: 1   MVSIGGISLPGSIPPLLHHQSSPTSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIANG 60

Query: 61  KVDGLGKSECETDFDTGLGNGRAGNLGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120
           KVDGLGKSECETDFDT LGNGRAGN GKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ
Sbjct: 61  KVDGLGKSECETDFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAKG 240
           RRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAKG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGGSPSEMAEKVMSYLEQKGFLQA 297
           LYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGGSPSEMAEKV+SYLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGGSPSEMAEKVLSYLEQKGFLQA 296

[Close]

BLAST of CSPI04G00220 vs. ExPASy TrEMBL
Match: A0A1S3BYH9 (Adenylyl-sulfate kinase OS=Cucumis melo OX=3656 GN=LOC103494812 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.5e-160
Identity = 282/296 (95.27%), Postives = 288/296 (97.30%), Query Frame = 0


Query: 1   MVSIGGISLPGSIPPSLHHQSSPTSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60
           MVSIG ISLPGS+PPSLHHQ SP SAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG
Sbjct: 1   MVSIGRISLPGSVPPSLHHQCSPPSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60

Query: 61  KVDGLGKSECETDFDTGLGNGRAGNLGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120
           KVDGLGKSECET+FDT LGNGRAGN GKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ
Sbjct: 61  KVDGLGKSECETEFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWFTGLSGSGKSTVACALSQSL KMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWFTGLSGSGKSTVACALSQSLCKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAKG 240
           RRVGEVAKLFADAGVICIASLISPYRR+RDACR ILP+GYFIEVFMDVPLEVCEARDAKG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGGSPSEMAEKVMSYLEQKGFLQA 297
           LYKLARAGKIKGFTGIDDPYEVP NCEIVLKHTGGSPSEMAEKV++YLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHTGGSPSEMAEKVLNYLEQKGFLQA 296

[Close]

BLAST of CSPI04G00220 vs. ExPASy TrEMBL
Match: A0A5A7TN80 (Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00500 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.5e-160
Identity = 282/296 (95.27%), Postives = 288/296 (97.30%), Query Frame = 0


Query: 1   MVSIGGISLPGSIPPSLHHQSSPTSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60
           MVSIG ISLPGS+PPSLHHQ SP SAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG
Sbjct: 1   MVSIGRISLPGSVPPSLHHQCSPPSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60

Query: 61  KVDGLGKSECETDFDTGLGNGRAGNLGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120
           KVDGLGKSECET+FDT LGNGRAGN GKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ
Sbjct: 61  KVDGLGKSECETEFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWFTGLSGSGKSTVACALSQSL KMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWFTGLSGSGKSTVACALSQSLCKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAKG 240
           RRVGEVAKLFADAGVICIASLISPYRR+RDACR ILP+GYFIEVFMDVPLEVCEARDAKG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGGSPSEMAEKVMSYLEQKGFLQA 297
           LYKLARAGKIKGFTGIDDPYEVP NCEIVLKHTGGSPSEMAEKV++YLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHTGGSPSEMAEKVLNYLEQKGFLQA 296

[Close]

BLAST of CSPI04G00220 vs. ExPASy TrEMBL
Match: A0A5D3DZZ6 (Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00510 PE=3 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 1.9e-159
Identity = 281/295 (95.25%), Postives = 287/295 (97.29%), Query Frame = 0


Query: 1   MVSIGGISLPGSIPPSLHHQSSPTSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60
           MVSIG ISLPGS+PPSLHHQ SP SAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG
Sbjct: 1   MVSIGRISLPGSVPPSLHHQCSPPSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60

Query: 61  KVDGLGKSECETDFDTGLGNGRAGNLGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120
           KVDGLGKSECET+FDT LGNGRAGN GKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ
Sbjct: 61  KVDGLGKSECETEFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWFTGLSGSGKSTVACALSQSL KMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWFTGLSGSGKSTVACALSQSLCKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAKG 240
           RRVGEVAKLFADAGVICIASLISPYRR+RDACR ILP+GYFIEVFMDVPLEVCEARDAKG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGGSPSEMAEKVMSYLEQKGFLQ 296
           LYKLARAGKIKGFTGIDDPYEVP NCEIVLKHTGGSPSEMAEKV++YLEQKGFLQ
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHTGGSPSEMAEKVLNYLEQKGFLQ 295

[Close]

BLAST of CSPI04G00220 vs. ExPASy TrEMBL
Match: A0A6J1J8X7 (Adenylyl-sulfate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482821 PE=3 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.5e-140
Identity = 250/297 (84.18%), Postives = 267/297 (89.90%), Query Frame = 0


Query: 1   MVSIGGISLPGSIPPSLHHQS-SPTSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVN 60
           MVS GGIS PG +PP L H+S SP SA GFARF + ISF+S+VV+ D  EKKGSR VIVN
Sbjct: 1   MVSTGGISFPGCVPPPLRHKSFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN 60

Query: 61  GKVDGLGKSECETDFDTGLGNGRAGNLGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLK 120
           GKVDGLGKSECE D D  LGNG AGN GKN GVLSTVGNSTNIKWHECS+G  E+QSLL 
Sbjct: 61  GKVDGLGKSECEIDLDATLGNGHAGNSGKNAGVLSTVGNSTNIKWHECSIGNVERQSLLN 120

Query: 121 QKGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAEN 180
           QKGCVIW TGLSGSGKS+VACAL++SLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAEN
Sbjct: 121 QKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAEN 180

Query: 181 IRRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAK 240
           IRRVGEVAKLFADAGVICIASLISPYRR+RDACR ILP+GYFIEVFMDVPLE+CEARD K
Sbjct: 181 IRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEICEARDTK 240

Query: 241 GLYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGGSPSEMAEKVMSYLEQKGFLQA 297
           GLYKLARAGKIKGFTGIDDPYEVP NCEIVLKH GGSP +MA+KVMSYLEQKGFL+A
Sbjct: 241 GLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSYLEQKGFLKA 297

[Close]

BLAST of CSPI04G00220 vs. NCBI nr
Match: XP_031739526.1 (adenylyl-sulfate kinase 3 [Cucumis sativus] >KGN52751.1 hypothetical protein Csa_014768 [Cucumis sativus])

HSP 1 Score: 591.7 bits (1524), Expect = 3.6e-165
Identity = 291/296 (98.31%), Postives = 292/296 (98.65%), Query Frame = 0


Query: 1   MVSIGGISLPGSIPPSLHHQSSPTSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60
           MVSIGGISLPGSIPP LHHQSSPTSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVI NG
Sbjct: 1   MVSIGGISLPGSIPPLLHHQSSPTSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIANG 60

Query: 61  KVDGLGKSECETDFDTGLGNGRAGNLGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120
           KVDGLGKSECETDFDT LGNGRAGN GKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ
Sbjct: 61  KVDGLGKSECETDFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAKG 240
           RRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAKG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGGSPSEMAEKVMSYLEQKGFLQA 297
           LYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGGSPSEMAEKV+SYLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGGSPSEMAEKVLSYLEQKGFLQA 296

[Close]

BLAST of CSPI04G00220 vs. NCBI nr
Match: XP_008454396.1 (PREDICTED: adenylyl-sulfate kinase 3 [Cucumis melo] >XP_008454397.1 PREDICTED: adenylyl-sulfate kinase 3 [Cucumis melo] >KAA0044328.1 adenylyl-sulfate kinase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 573.2 bits (1476), Expect = 1.3e-159
Identity = 282/296 (95.27%), Postives = 288/296 (97.30%), Query Frame = 0


Query: 1   MVSIGGISLPGSIPPSLHHQSSPTSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60
           MVSIG ISLPGS+PPSLHHQ SP SAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG
Sbjct: 1   MVSIGRISLPGSVPPSLHHQCSPPSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60

Query: 61  KVDGLGKSECETDFDTGLGNGRAGNLGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120
           KVDGLGKSECET+FDT LGNGRAGN GKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ
Sbjct: 61  KVDGLGKSECETEFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWFTGLSGSGKSTVACALSQSL KMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWFTGLSGSGKSTVACALSQSLCKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAKG 240
           RRVGEVAKLFADAGVICIASLISPYRR+RDACR ILP+GYFIEVFMDVPLEVCEARDAKG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGGSPSEMAEKVMSYLEQKGFLQA 297
           LYKLARAGKIKGFTGIDDPYEVP NCEIVLKHTGGSPSEMAEKV++YLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHTGGSPSEMAEKVLNYLEQKGFLQA 296

[Close]

BLAST of CSPI04G00220 vs. NCBI nr
Match: TYK29457.1 (adenylyl-sulfate kinase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 571.6 bits (1472), Expect = 3.9e-159
Identity = 281/295 (95.25%), Postives = 287/295 (97.29%), Query Frame = 0


Query: 1   MVSIGGISLPGSIPPSLHHQSSPTSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60
           MVSIG ISLPGS+PPSLHHQ SP SAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG
Sbjct: 1   MVSIGRISLPGSVPPSLHHQCSPPSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60

Query: 61  KVDGLGKSECETDFDTGLGNGRAGNLGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120
           KVDGLGKSECET+FDT LGNGRAGN GKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ
Sbjct: 61  KVDGLGKSECETEFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWFTGLSGSGKSTVACALSQSL KMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWFTGLSGSGKSTVACALSQSLCKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAKG 240
           RRVGEVAKLFADAGVICIASLISPYRR+RDACR ILP+GYFIEVFMDVPLEVCEARDAKG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGGSPSEMAEKVMSYLEQKGFLQ 296
           LYKLARAGKIKGFTGIDDPYEVP NCEIVLKHTGGSPSEMAEKV++YLEQKGFLQ
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHTGGSPSEMAEKVLNYLEQKGFLQ 295

[Close]

BLAST of CSPI04G00220 vs. NCBI nr
Match: XP_038903366.1 (adenylyl-sulfate kinase 3-like [Benincasa hispida])

HSP 1 Score: 555.1 bits (1429), Expect = 3.8e-154
Identity = 274/296 (92.57%), Postives = 281/296 (94.93%), Query Frame = 0


Query: 1   MVSIGGISLPGSIPPSLHHQSSPTSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60
           MV+IGGISLPG +PPSLHHQS   SAIGFARFPDRISFRSSVV RD VEKK SRVVIVNG
Sbjct: 1   MVAIGGISLPGFLPPSLHHQSFSPSAIGFARFPDRISFRSSVVWRDGVEKKDSRVVIVNG 60

Query: 61  KVDGLGKSECETDFDTGLGNGRAGNLGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120
           KVDGLGKSECET+FDT LGNG AGN GKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ
Sbjct: 61  KVDGLGKSECETEFDTTLGNGHAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIW TGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWITGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAKG 240
           RRVGEVAKLFADAGVICIASLISPYRR+RDACR ILP+GYFIEVFMDVPLEVCEARDAKG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGGSPSEMAEKVMSYLEQKGFLQA 297
           LYKLARAGKIKGFTGIDDPYEVP NCEI+LKH GGSPSEMA KV+SYLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEILLKHNGGSPSEMAGKVLSYLEQKGFLQA 296

[Close]

BLAST of CSPI04G00220 vs. NCBI nr
Match: XP_022984565.1 (adenylyl-sulfate kinase 3-like [Cucurbita maxima])

HSP 1 Score: 508.8 bits (1309), Expect = 3.1e-140
Identity = 250/297 (84.18%), Postives = 267/297 (89.90%), Query Frame = 0


Query: 1   MVSIGGISLPGSIPPSLHHQS-SPTSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVN 60
           MVS GGIS PG +PP L H+S SP SA GFARF + ISF+S+VV+ D  EKKGSR VIVN
Sbjct: 1   MVSTGGISFPGCVPPPLRHKSFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN 60

Query: 61  GKVDGLGKSECETDFDTGLGNGRAGNLGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLK 120
           GKVDGLGKSECE D D  LGNG AGN GKN GVLSTVGNSTNIKWHECS+G  E+QSLL 
Sbjct: 61  GKVDGLGKSECEIDLDATLGNGHAGNSGKNAGVLSTVGNSTNIKWHECSIGNVERQSLLN 120

Query: 121 QKGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAEN 180
           QKGCVIW TGLSGSGKS+VACAL++SLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAEN
Sbjct: 121 QKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAEN 180

Query: 181 IRRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAK 240
           IRRVGEVAKLFADAGVICIASLISPYRR+RDACR ILP+GYFIEVFMDVPLE+CEARD K
Sbjct: 181 IRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEICEARDTK 240

Query: 241 GLYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGGSPSEMAEKVMSYLEQKGFLQA 297
           GLYKLARAGKIKGFTGIDDPYEVP NCEIVLKH GGSP +MA+KVMSYLEQKGFL+A
Sbjct: 241 GLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSYLEQKGFLKA 297

[Close]

BLAST of CSPI04G00220 vs. TAIR 10
Match: AT2G14750.1 (APS kinase )

HSP 1 Score: 353.6 bits (906), Expect = 1.5e-97
Identity = 171/214 (79.91%), Postives = 185/214 (86.45%), Query Frame = 0


Query: 84  GNLGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQKGCVIWFTGLSGSGKSTVACALS 143
           G+ G+  G LSTVGNSTNIKWHECSV K ++Q LL QKGCVIW TGLSGSGKST+ACAL+
Sbjct: 63  GHTGQKQGPLSTVGNSTNIKWHECSVEKVDRQRLLDQKGCVIWVTGLSGSGKSTLACALN 122

Query: 144 QSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLIS 203
           Q LY+ GKL YILDGDNVRHGLNRDL FKAEDRAENIRRVGEVAKLFADAG+ICIASLIS
Sbjct: 123 QMLYQKGKLCYILDGDNVRHGLNRDLSFKAEDRAENIRRVGEVAKLFADAGIICIASLIS 182

Query: 204 PYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVP 263
           PYR +RDACR +LP G F+EVFMDVPL VCEARD KGLYKLARAGKIKGFTGIDDPYE P
Sbjct: 183 PYRTDRDACRSLLPEGDFVEVFMDVPLSVCEARDPKGLYKLARAGKIKGFTGIDDPYEPP 242

Query: 264 FNCEIVLKHTGG-SPSEMAEKVMSYLEQKGFLQA 297
            NCEI L   GG SP EMAEKV+ YL+ KG+LQA
Sbjct: 243 LNCEISLGREGGTSPIEMAEKVVGYLDNKGYLQA 276

[Close]

BLAST of CSPI04G00220 vs. TAIR 10
Match: AT3G03900.1 (adenosine-5'-phosphosulfate (APS) kinase 3 )

HSP 1 Score: 323.2 bits (827), Expect = 2.2e-88
Identity = 154/206 (74.76%), Postives = 176/206 (85.44%), Query Frame = 0


Query: 93  LSTVGNSTNIKWHECSVGKNEKQSLLKQKGCVIWFTGLSGSGKSTVACALSQSLYKMGKL 152
           +STVGNSTNI W E  +GK E+Q LL QKGCV+W TGLSGSGKST+AC+LS+ L   GKL
Sbjct: 1   MSTVGNSTNIFWQESPIGKTERQKLLNQKGCVVWITGLSGSGKSTLACSLSRELNNRGKL 60

Query: 153 AYILDGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRERDAC 212
           +YILDGDN+RHGLN+DLGFKAEDR ENIRRVGEVAKLFADAG+ICIASLISPYR++RDAC
Sbjct: 61  SYILDGDNLRHGLNKDLGFKAEDRVENIRRVGEVAKLFADAGLICIASLISPYRKDRDAC 120

Query: 213 RDILPNGYFIEVFMDVPLEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVPFNCEIVLKH 272
           R+++ N  FIEVFM++ L++CEARD KGLYKLARAGKIKGFTGIDDPYE P NCEI LK 
Sbjct: 121 REMIQNSSFIEVFMNMSLQLCEARDPKGLYKLARAGKIKGFTGIDDPYESPLNCEIELKE 180

Query: 273 TGG---SPSEMAEKVMSYLEQKGFLQ 296
             G   SP  MAE+V+SYLE KGFLQ
Sbjct: 181 KEGECPSPVAMAEEVISYLEDKGFLQ 206

[Close]

BLAST of CSPI04G00220 vs. TAIR 10
Match: AT4G39940.1 (APS-kinase 2 )

HSP 1 Score: 320.9 bits (821), Expect = 1.1e-87
Identity = 152/213 (71.36%), Postives = 178/213 (83.57%), Query Frame = 0


Query: 87  GKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQKGCVIWFTGLSGSGKSTVACALSQSL 146
           G+N+G       + NI WHE S+ + ++Q LL+QKGCV+W TGLSGSGKSTVACALS++L
Sbjct: 84  GRNNG----KEKAENIVWHESSICRCDRQQLLQQKGCVVWITGLSGSGKSTVACALSKAL 143

Query: 147 YKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYR 206
           ++ GKL Y LDGDNVRHGLNRDL FKAE R ENIRR+GEVAKLFAD GVICIASLISPYR
Sbjct: 144 FERGKLTYTLDGDNVRHGLNRDLTFKAEHRTENIRRIGEVAKLFADVGVICIASLISPYR 203

Query: 207 RERDACRDILPNGYFIEVFMDVPLEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVPFNC 266
           R+RDACR +LP+G F+EVFMDVPL VCE+RD KGLYKLARAGKIKGFTGIDDPYE P NC
Sbjct: 204 RDRDACRSLLPDGDFVEVFMDVPLHVCESRDPKGLYKLARAGKIKGFTGIDDPYEAPVNC 263

Query: 267 EIVLKHTGG----SPSEMAEKVMSYLEQKGFLQ 296
           E+VLKHTG     SP +MAE ++SYL+ KG+L+
Sbjct: 264 EVVLKHTGDDESCSPRQMAENIISYLQNKGYLE 292

[Close]

BLAST of CSPI04G00220 vs. TAIR 10
Match: AT5G67520.1 (adenosine-5'-phosphosulfate (APS) kinase 4 )

HSP 1 Score: 301.6 bits (771), Expect = 7.0e-82
Identity = 146/217 (67.28%), Postives = 173/217 (79.72%), Query Frame = 0


Query: 97  GNSTNIKWHECSVGKNEKQSLLKQKGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYIL 156
           G   NI WH+C V K+++Q L+KQKGCVIW TGLSGSGKS++ACALS++L+  GKL+YIL
Sbjct: 84  GKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGSGKSSLACALSRALHNRGKLSYIL 143

Query: 157 DGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRERDACRDIL 216
           DGDNVRHGLN DL F+A+DRAENIRRVGEVAKLFAD+G+ICIASLISPYR ER ACR +L
Sbjct: 144 DGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICIASLISPYRIERAACRALL 203

Query: 217 PNGYFIEVFMDVPLEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVPFNCEIVLKH---- 276
           P G FIEVFMDVPL VCEARD KGLYK ARAGKIKGFTG+DDPYE P +CEIV+++    
Sbjct: 204 PQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDDPYEAPLDCEIVIQNSRDK 263

Query: 277 -------TGGSPS-------EMAEKVMSYLEQKGFLQ 296
                  +  SPS       EMA+ V+SYL+Q G+L+
Sbjct: 264 GLSSSSSSSSSPSSSSSSLCEMADIVVSYLDQNGYLK 300

The following BLAST results are available for this feature:

BLAST of CSPI04G00220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43295	2.2e-96	79.91	Adenylyl-sulfate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK1...	[more]
O49204	2.0e-89	68.98	Adenylyl-sulfate kinase, chloroplastic OS=Catharanthus roseus OX=4058 GN=AKN PE=...	[more]
Q9SRW7	3.2e-87	74.76	Adenylyl-sulfate kinase 3 OS=Arabidopsis thaliana OX=3702 GN=APK3 PE=1 SV=1	[more]
O49196	1.6e-86	71.36	Adenylyl-sulfate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK2...	[more]
Q84JF0	9.8e-81	67.28	Adenylyl-sulfate kinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK4...	[more]

BLAST of CSPI04G00220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KY24	1.8e-165	98.31	Adenylyl-sulfate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G000720 PE=3 SV=1	[more]
A0A1S3BYH9	6.5e-160	95.27	Adenylyl-sulfate kinase OS=Cucumis melo OX=3656 GN=LOC103494812 PE=3 SV=1	[more]
A0A5A7TN80	6.5e-160	95.27	Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A5D3DZZ6	1.9e-159	95.25	Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A6J1J8X7	1.5e-140	84.18	Adenylyl-sulfate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482821 PE=3 SV=1	[more]

BLAST of CSPI04G00220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031739526.1	3.6e-165	98.31	adenylyl-sulfate kinase 3 [Cucumis sativus] >KGN52751.1 hypothetical protein Csa...	[more]
XP_008454396.1	1.3e-159	95.27	PREDICTED: adenylyl-sulfate kinase 3 [Cucumis melo] >XP_008454397.1 PREDICTED: a...	[more]
TYK29457.1	3.9e-159	95.25	adenylyl-sulfate kinase 3 [Cucumis melo var. makuwa]	[more]
XP_038903366.1	3.8e-154	92.57	adenylyl-sulfate kinase 3-like [Benincasa hispida]	[more]
XP_022984565.1	3.1e-140	84.18	adenylyl-sulfate kinase 3-like [Cucurbita maxima]	[more]

BLAST of CSPI04G00220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G14750.1	1.5e-97	79.91	APS kinase	[more]
AT3G03900.1	2.2e-88	74.76	adenosine-5'-phosphosulfate (APS) kinase 3	[more]
AT4G39940.1	1.1e-87	71.36	APS-kinase 2	[more]
AT5G67520.1	7.0e-82	67.28	adenosine-5'-phosphosulfate (APS) kinase 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF01583	APS_kinase	coord: 121..273 e-value: 4.8E-72 score: 241.0
None	No IPR available	PANTHER	PTHR11055:SF55	ADENYLYL-SULFATE KINASE 1, CHLOROPLASTIC	coord: 38..296
None	No IPR available	PANTHER	PTHR11055	BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE	coord: 38..296
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 93..296 e-value: 6.5E-94 score: 315.4
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 120..291
IPR002891	Adenylyl-sulfate kinase	TIGRFAM	TIGR00455	TIGR00455	coord: 104..287 e-value: 2.7E-76 score: 253.3
IPR002891	Adenylyl-sulfate kinase	HAMAP	MF_00065	Adenylyl_sulf_kinase	coord: 120..292 score: 36.437248
IPR002891	Adenylyl-sulfate kinase	CDD	cd02027	APSK	coord: 124..271 e-value: 2.262E-99 score: 286.296

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G00220.1	CSPI04G00220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070814	hydrogen sulfide biosynthetic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0000103	sulfate assimilation
molecular_function	GO:0004020	adenylylsulfate kinase activity
molecular_function	GO:0005524	ATP binding