CSPI03G47550 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G47550
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	low-temperature-induced 65 kDa protein
Location	Chr3: 40533540 .. 40535860 (+)
RNA-Seq Expression	CSPI03G47550
Synteny	CSPI03G47550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCTAATTAACATCCCCAATTTCATCCATTTCCTTTCATTATCATGGACTCACAAATCCCCCCTCATCACCATCACCATCACCACCATCCCCTTGGACTTCACCAAAGTATGACTTTCTCTGTTTCCTAACTTTTTCTTTTTTATTTTAAATAACTTAAGTTCATCATTTTATTTTAATTTCTTTTATGTCAAATTACATATTTGATCCATTTAAATTTGTATTTAGTTTAAATTTTAATTATAAATTTACACTAGTATCTAGACCTATTCAAATTCTATAATTAAATTGTAGGGTGAAGTTTACTTTTCTTTAGGAATTGTGAAAGTTAACACTACTACTTGATCATGGGGCAAACATTCCTGACTAGGTTCCACACATATCAATTACGAATGTTTATATTTCATTAGACTAATAAAGAAAATTATATACAAATTAAAAAGATTTAGCATTGGTAGGTCATGTGGAGGGAAAAGAAGAACTACAAGAAGGGGACCAGGAGCATCATGAGAAGAAGTCAGTGTTGAAGAAAGTGAAGGCGAAAGCTAAAAAGATTAAAGACACTATTACGAAGCACGGCCATGGCCATGATCATCATGACGAAGAAGACGTCAGTGATGACGAGGAGGATGAAGTTGTAGAAGATCCCGAAATCCAGGGTGCACCATGTATATACACATCTTCCTTTTCTTTCATAATTATAAATTGATGTAGGTGTTGTCAATTTGAAGAAGAGAATGTTAACTAAAGTACATTTTGACAGTATATGAAGGTGCTGCAATGAGGAGTGCCGTGGCTGGGAAAGGGCAGCATCAAGATGTCGGAATTAGAACTGGAACGACGAGAATGATGCACAATGAGCCGCCTCCAGCTCGAAGGGAAATCACAAGTCGTCCCTCTGCTGTTGACACTGGCTTTACTTCCATCCATAATCCCACAGCAAATTATAAAGTAGACGACTCCAATGTTGCCCCGAACACCACCATGTCTCTATCTCCATGGAAATTGGAGGAGGATCCTCACGCGCCTAAGGACCCTCATGCTCCTCATATCTCCGAGGTCAAAGTTCACGATCCCTCTAACAGAGGTAATTAGATCCATACATAAGACTTTTTTCATCATCATATTTTATTTTAGAGCTCAATTCGAGGCTCATGGTACGTGTGTTATTTTGTTTAGAGCTTTGAATCTCATTCCATGATATCCAACTTTCTCGCTTTTTATTTGGGTACACATTTTATTATGCTTATCAAAATAAGCTGGCTTGCAAATTTTGTCCTATTTTTAGTTTCCACTGAACTTACTTATAGTGAGTCCTTTACGTCTTCAAACCACACTACAGAGTAAAGAGTCAACCAATCGAAGTTAGTAAAAAAATTAATCTAAAACTCATGACTATAAGGTGATCTTTTGTTTTCGTTTTTTGGGTTTTTTTTTTTTTTATCTCATGAACATGTCTGCAACGTACAGGGAGCGAAGAGGCAGCAGGGAGATCTCAAGTCTTCGATTCGTTTGCAAGAATGAAAGTTAACGACAAAGAACCGAACCGCACCGGTTCACCACGTGGATTAGTCCAAGAACAAGGAGGAGAAGACCAGACCAACTACGCCCAAAAAATCTCAGCCGTGGGTTCAGCAGTGAGTGGAAAAGCAGTAGCAGCGAAGGATTTTGTGGCATCAAAGCTAGGGTATGGAGAAACAACTGAGGAAACCACCACCAACAAGTCATCTTCGTCTTCACCGTTAGAGTACGGCAAAAAGATAGCATTAACAGTGACAGAGAAGTTAAAGCCAGGGGAAGAAGACAGAGCATTGTCAGAAGTAATTTCAGAGGCTTGGACTAGAAGGAAGGATGAGGTAGTTAAAGTTGGAGAAAGTGCTTTTGGACGGCCACCCTCTAAAGGGGAGGTGACGGAGTCGGAGGAGTTGACACGGCGGCTTGGGAAAGAGGATAAGGAAGCGACAGAGAAATCCTCTGCTGCGAGGGCTGCAGCAGCGACAGGGAGGAGTGTGGTGGGTATGGTGAAAGATACGGTGGGGTCGTGGCTGGGAAATGCTGGAGAGCAATCGGTGCCATCGCAACAATCACTTGGTACTTCACAAGGTAACTTAACTAGTGTCTAGTTAAGGGTTTTGTGATTGTTTGGTTTTATCTGTTAATTTCATATTTTTGTGGGAATAATTTGAAAAGGTGTCGAGGGTTTTGTGGATTCTTCTTCATCACGGCGGCAGGCGGAGCATGGTGGTAAGGGGGCGGAGGTGCGGAGACTTCAGGGTTCGGCGAATTAA

mRNA sequence

CCCTAATTAACATCCCCAATTTCATCCATTTCCTTTCATTATCATGGACTCACAAATCCCCCCTCATCACCATCACCATCACCACCATCCCCTTGGACTTCACCAAAGTCATGTGGAGGGAAAAGAAGAACTACAAGAAGGGGACCAGGAGCATCATGAGAAGAAGTCAGTGTTGAAGAAAGTGAAGGCGAAAGCTAAAAAGATTAAAGACACTATTACGAAGCACGGCCATGGCCATGATCATCATGACGAAGAAGACGTCAGTGATGACGAGGAGGATGAAGTTGTAGAAGATCCCGAAATCCAGGGTGCACCATTATATGAAGGTGCTGCAATGAGGAGTGCCGTGGCTGGGAAAGGGCAGCATCAAGATGTCGGAATTAGAACTGGAACGACGAGAATGATGCACAATGAGCCGCCTCCAGCTCGAAGGGAAATCACAAGTCGTCCCTCTGCTGTTGACACTGGCTTTACTTCCATCCATAATCCCACAGCAAATTATAAAGTAGACGACTCCAATGTTGCCCCGAACACCACCATGTCTCTATCTCCATGGAAATTGGAGGAGGATCCTCACGCGCCTAAGGACCCTCATGCTCCTCATATCTCCGAGGTCAAAGTTCACGATCCCTCTAACAGAGGGAGCGAAGAGGCAGCAGGGAGATCTCAAGTCTTCGATTCGTTTGCAAGAATGAAAGTTAACGACAAAGAACCGAACCGCACCGGTTCACCACGTGGATTAGTCCAAGAACAAGGAGGAGAAGACCAGACCAACTACGCCCAAAAAATCTCAGCCGTGGGTTCAGCAGTGAGTGGAAAAGCAGTAGCAGCGAAGGATTTTGTGGCATCAAAGCTAGGGTATGGAGAAACAACTGAGGAAACCACCACCAACAAGTCATCTTCGTCTTCACCGTTAGAGTACGGCAAAAAGATAGCATTAACAGTGACAGAGAAGTTAAAGCCAGGGGAAGAAGACAGAGCATTGTCAGAAGTAATTTCAGAGGCTTGGACTAGAAGGAAGGATGAGGTAGTTAAAGTTGGAGAAAGTGCTTTTGGACGGCCACCCTCTAAAGGGGAGGTGACGGAGTCGGAGGAGTTGACACGGCGGCTTGGGAAAGAGGATAAGGAAGCGACAGAGAAATCCTCTGCTGCGAGGGCTGCAGCAGCGACAGGGAGGAGTGTGGTGGGTATGGTGAAAGATACGGTGGGGTCGTGGCTGGGAAATGCTGGAGAGCAATCGGTGCCATCGCAACAATCACTTGGTACTTCACAAGGTGTCGAGGGTTTTGTGGATTCTTCTTCATCACGGCGGCAGGCGGAGCATGGTGGTAAGGGGGCGGAGGTGCGGAGACTTCAGGGTTCGGCGAATTAA

Coding sequence (CDS)

ATGGACTCACAAATCCCCCCTCATCACCATCACCATCACCACCATCCCCTTGGACTTCACCAAAGTCATGTGGAGGGAAAAGAAGAACTACAAGAAGGGGACCAGGAGCATCATGAGAAGAAGTCAGTGTTGAAGAAAGTGAAGGCGAAAGCTAAAAAGATTAAAGACACTATTACGAAGCACGGCCATGGCCATGATCATCATGACGAAGAAGACGTCAGTGATGACGAGGAGGATGAAGTTGTAGAAGATCCCGAAATCCAGGGTGCACCATTATATGAAGGTGCTGCAATGAGGAGTGCCGTGGCTGGGAAAGGGCAGCATCAAGATGTCGGAATTAGAACTGGAACGACGAGAATGATGCACAATGAGCCGCCTCCAGCTCGAAGGGAAATCACAAGTCGTCCCTCTGCTGTTGACACTGGCTTTACTTCCATCCATAATCCCACAGCAAATTATAAAGTAGACGACTCCAATGTTGCCCCGAACACCACCATGTCTCTATCTCCATGGAAATTGGAGGAGGATCCTCACGCGCCTAAGGACCCTCATGCTCCTCATATCTCCGAGGTCAAAGTTCACGATCCCTCTAACAGAGGGAGCGAAGAGGCAGCAGGGAGATCTCAAGTCTTCGATTCGTTTGCAAGAATGAAAGTTAACGACAAAGAACCGAACCGCACCGGTTCACCACGTGGATTAGTCCAAGAACAAGGAGGAGAAGACCAGACCAACTACGCCCAAAAAATCTCAGCCGTGGGTTCAGCAGTGAGTGGAAAAGCAGTAGCAGCGAAGGATTTTGTGGCATCAAAGCTAGGGTATGGAGAAACAACTGAGGAAACCACCACCAACAAGTCATCTTCGTCTTCACCGTTAGAGTACGGCAAAAAGATAGCATTAACAGTGACAGAGAAGTTAAAGCCAGGGGAAGAAGACAGAGCATTGTCAGAAGTAATTTCAGAGGCTTGGACTAGAAGGAAGGATGAGGTAGTTAAAGTTGGAGAAAGTGCTTTTGGACGGCCACCCTCTAAAGGGGAGGTGACGGAGTCGGAGGAGTTGACACGGCGGCTTGGGAAAGAGGATAAGGAAGCGACAGAGAAATCCTCTGCTGCGAGGGCTGCAGCAGCGACAGGGAGGAGTGTGGTGGGTATGGTGAAAGATACGGTGGGGTCGTGGCTGGGAAATGCTGGAGAGCAATCGGTGCCATCGCAACAATCACTTGGTACTTCACAAGGTGTCGAGGGTTTTGTGGATTCTTCTTCATCACGGCGGCAGGCGGAGCATGGTGGTAAGGGGGCGGAGGTGCGGAGACTTCAGGGTTCGGCGAATTAA

Protein sequence

MDSQIPPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDTITKHGHGHDHHDEEDVSDDEEDEVVEDPEIQGAPLYEGAAMRSAVAGKGQHQDVGIRTGTTRMMHNEPPPARREITSRPSAVDTGFTSIHNPTANYKVDDSNVAPNTTMSLSPWKLEEDPHAPKDPHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARMKVNDKEPNRTGSPRGLVQEQGGEDQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETTEETTTNKSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLGKEDKEATEKSSAARAAAATGRSVVGMVKDTVGSWLGNAGEQSVPSQQSLGTSQGVEGFVDSSSSRRQAEHGGKGAEVRRLQGSAN*

Homology

[Close]

BLAST of CSPI03G47550 vs. ExPASy Swiss-Prot
Match: Q04980 (Low-temperature-induced 65 kDa protein OS=Arabidopsis thaliana OX=3702 GN=LTI65 PE=2 SV=2)

HSP 1 Score: 101.3 bits (251), Expect = 2.9e-20
Identity = 181/646 (28.02%), Postives = 248/646 (38.39%), Query Frame = 0


Query: 1   MDSQIP-PHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEK--KSVLKKVKAKAKKIKDT 60
           M+SQ+  P+ H     P+ +H  H E        ++EHHEK    VLKKVK KAKKIK++
Sbjct: 1   MESQLTRPYGHEQAEEPIRIH--HPE--------EEEHHEKGASKVLKKVKEKAKKIKNS 60

Query: 61  ITKHGHGHDHHDEEDVSDDEEDEVVEDPEIQGAPLYEGAAMRSAVAGKGQHQDVGIRTGT 120
           +TKHG+GHDH  E+D  DDE DE  +DPE+ GAP+YE +A+R  V GK +       T  
Sbjct: 61  LTKHGNGHDHDVEDD--DDEYDE--QDPEVHGAPVYESSAVRGGVTGKPKSLSHAGETNV 120

Query: 121 TRMMHNEPP-----PARREITSRP----SAVDT--GFTSIHNPTANYKVDD---SNVAP- 180
                  PP     P      ++P    S  DT  G  ++ +P    +  D      AP 
Sbjct: 121 PASEEIVPPGTKVFPVVSSDHTKPIEPVSLQDTSYGHEALADPVRTTETSDWEAKREAPT 180

Query: 181 -----------------------NTTMSL-----------------------------SP 240
                                  NT +SL                             +P
Sbjct: 181 HYPLGVSEFSDRGESREAHQEPLNTPVSLLSATEDVTRTFAPGGEDDYLGGQRKVNVETP 240

Query: 241 WKLEEDPHAPKDPHAPHISEV-----KVHDPSNRGSEEAAGRSQVFDSFARMKVNDKEPN 300
            +LEEDP AP    + ++S V     KV DP+++G E  AG  ++ +S  RMKV D+ P+
Sbjct: 241 KRLEEDPAAP-GGGSDYLSGVSNYQSKVTDPTHKGGE--AGVPEIAESLGRMKVTDESPD 300

Query: 301 R--------------------------TGSP-----------------RGLVQEQGG--- 360
           +                            SP                 RG V+ + G   
Sbjct: 301 QKSRQGREEDFPTRSHEFDLKKESDINKNSPARFGGESKAGMEEDFPTRGDVKVESGLGR 360

Query: 361 ------EDQ---------------------------TNYAQKISAVGSAVSGKAVAAKDF 420
                  DQ                           + Y +++++  SA++ KA+AAK+ 
Sbjct: 361 DLPTGTHDQFSPELSRPKERDDSEETKDESTHETKPSTYTEQLASATSAITNKAIAAKNV 420

Query: 421 VASKLGY----GETTEETTTNKSSSSSPLEYGKKIALTV--------------------- 434
           VASKLGY    G    E+     +  S   YG+K+A TV                     
Sbjct: 421 VASKLGYTGENGGGQSESPVKDETPRSVTAYGQKVAGTVAEKLTPVYEKVKETGSTVMTK 480

[Close]

BLAST of CSPI03G47550 vs. ExPASy TrEMBL
Match: A0A1S3CPX7 (low-temperature-induced 65 kDa protein OS=Cucumis melo OX=3656 GN=LOC103503460 PE=4 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 6.8e-206
Identity = 396/443 (89.39%), Postives = 406/443 (91.65%), Query Frame = 0


Query: 1   MDSQIPPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDTITK 60
           MDSQIPP  HHHHHHPLGLHQS  EGKEEL+EG +EHHEKKSVLKKVKAKAKKIKDTITK
Sbjct: 1   MDSQIPP--HHHHHHPLGLHQS-AEGKEELEEG-EEHHEKKSVLKKVKAKAKKIKDTITK 60

Query: 61  HGHGHDHHDEEDVSDDEEDEVVEDPEIQGAPLYEGAAMRSAVAGKGQHQDVGIRTGTTRM 120
           H H HDHHDEED  DDEEDEVVEDPEIQGAPLYEGAAMRSA AGKGQHQDVGI  GTT M
Sbjct: 61  HAHAHDHHDEEDEEDDEEDEVVEDPEIQGAPLYEGAAMRSATAGKGQHQDVGIGIGTTTM 120

Query: 121 MHN-EPPPARREITSRPSAVDTGFTSIHNPTANYKVDDSNVAPNTTMSLSPWKLEEDPHA 180
           MHN  PPPARREI SRPSAVDTGFTS+ NPTAN+KVDDSNVAPNTTMSLSPWKLEEDPH 
Sbjct: 121 MHNYAPPPARREIRSRPSAVDTGFTSVENPTANHKVDDSNVAPNTTMSLSPWKLEEDPHG 180

Query: 181 PKDPHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARMKVNDKEPNRTGSPRGLVQEQGG 240
           PKDP APHISEVKVHDPSNRGSEEAAG+SQVFDSFA+MKV+DK+PNRTGSP GL QE GG
Sbjct: 181 PKDPRAPHISEVKVHDPSNRGSEEAAGKSQVFDSFAKMKVDDKKPNRTGSPHGLDQEHGG 240

Query: 241 EDQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETTEETTTNKSSSSSPLEYGKKIAL 300
           ED  NYAQKISAVGSAVSGKAVAAKDFVASKLGYGET E TT +KSSSSSPLEYGKKIAL
Sbjct: 241 EDHINYAQKISAVGSAVSGKAVAAKDFVASKLGYGETKEGTTADKSSSSSPLEYGKKIAL 300

Query: 301 TVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLGKE 360
           TVTEKLKPGEEDRALSEVISEA  RRKDEVVKVGESAFGRPPSKG VTESEELTRRLG+E
Sbjct: 301 TVTEKLKPGEEDRALSEVISEALNRRKDEVVKVGESAFGRPPSKGVVTESEELTRRLGEE 360

Query: 361 DKEATEKSSAARAAAATGRSVVGMVKDTVGSWLGNAGEQSVPSQQSLGTSQGVEGFVDSS 420
           DKEATEKSS A AAAATGRSVVGMVKDTVGSWLG AGEQS PSQQSLGTSQGVEGFVD S
Sbjct: 361 DKEATEKSSVASAAAATGRSVVGMVKDTVGSWLGKAGEQSAPSQQSLGTSQGVEGFVDDS 420

Query: 421 SSRRQAEHGGKGAEVRRLQGSAN 443
           SSRRQAEHGG GAEVR LQGSAN
Sbjct: 421 SSRRQAEHGGTGAEVRVLQGSAN 439

[Close]

BLAST of CSPI03G47550 vs. ExPASy TrEMBL
Match: A0A0A0LIE7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914030 PE=4 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 3.4e-181
Identity = 340/345 (98.55%), Postives = 342/345 (99.13%), Query Frame = 0


Query: 98  MRSAVAGKGQHQDVGIRTGTTRMMHNEPPPARREITSRPSAVDTGFTSIHNPTANYKVDD 157
           MRSAVAGKGQHQDVGI TGTTRMMHNEPPPARREITSRPSAVDTGFTSIHN TANYKVDD
Sbjct: 1   MRSAVAGKGQHQDVGIGTGTTRMMHNEPPPARREITSRPSAVDTGFTSIHNTTANYKVDD 60

Query: 158 SNVAPNTTMSLSPWKLEEDPHAPKDPHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARM 217
           SNVAPNTTMSLSPWKLE+DPHAPKDPHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARM
Sbjct: 61  SNVAPNTTMSLSPWKLEDDPHAPKDPHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARM 120

Query: 218 KVNDKEPNRTGSPRGLVQEQGGEDQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETT 277
           KV+DKEPNRTGSPRGLVQEQGGEDQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETT
Sbjct: 121 KVDDKEPNRTGSPRGLVQEQGGEDQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETT 180

Query: 278 EETTTNKSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAF 337
           EETTTNKSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAF
Sbjct: 181 EETTTNKSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAF 240

Query: 338 GRPPSKGEVTESEELTRRLGKEDKEATEKSSAARAAAATGRSVVGMVKDTVGSWLGNAGE 397
           GRPPSKGEVTESEELTRRLGKEDKEATEKSS ARAAAATGRSVVGMVKDTVGSWLGNAGE
Sbjct: 241 GRPPSKGEVTESEELTRRLGKEDKEATEKSSVARAAAATGRSVVGMVKDTVGSWLGNAGE 300

Query: 398 QSVPSQQSLGTSQGVEGFVDSSSSRRQAEHGGKGAEVRRLQGSAN 443
           QSVPSQQSLGTSQGVEGFVDSSSSRRQAEHGGKGAEVRRLQGSAN
Sbjct: 301 QSVPSQQSLGTSQGVEGFVDSSSSRRQAEHGGKGAEVRRLQGSAN 345

[Close]

BLAST of CSPI03G47550 vs. ExPASy TrEMBL
Match: A0A5D3E5L4 (Low-temperature-induced 65 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G003650 PE=4 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.4e-163
Identity = 315/352 (89.49%), Postives = 324/352 (92.05%), Query Frame = 0


Query: 92  LYEGAAMRSAVAGKGQHQDVGIRTGTTRMMHN-EPPPARREITSRPSAVDTGFTSIHNPT 151
           +YEGAAMRSA AGKGQHQDVGI  GTT MMHN  PPPARREI SRPSAVDTGFTS+ NPT
Sbjct: 8   VYEGAAMRSATAGKGQHQDVGIGIGTTTMMHNYAPPPARREIRSRPSAVDTGFTSVENPT 67

Query: 152 ANYKVDDSNVAPNTTMSLSPWKLEEDPHAPKDPHAPHISEVKVHDPSNRGSEEAAGRSQV 211
           AN+KVDDSNVAPNTTMSLSPWKLEEDPH PKDP APHISEVKVHDPSNRGSEEAAG+SQV
Sbjct: 68  ANHKVDDSNVAPNTTMSLSPWKLEEDPHGPKDPRAPHISEVKVHDPSNRGSEEAAGKSQV 127

Query: 212 FDSFARMKVNDKEPNRTGSPRGLVQEQGGEDQTNYAQKISAVGSAVSGKAVAAKDFVASK 271
           FDSFA+MKV+DK+PNRTGSP GL QE GGED  NYAQKISAVGSAVSGKAVAAKDFVASK
Sbjct: 128 FDSFAKMKVDDKKPNRTGSPHGLDQEHGGEDHINYAQKISAVGSAVSGKAVAAKDFVASK 187

Query: 272 LGYGETTEETTTNKSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVV 331
           LGYGET E TT +KSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEA  RRKDEVV
Sbjct: 188 LGYGETKEGTTADKSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEALNRRKDEVV 247

Query: 332 KVGESAFGRPPSKGEVTESEELTRRLGKEDKEATEKSSAARAAAATGRSVVGMVKDTVGS 391
           KVGESAFGRPPSKG VTESEELTRRLG+EDKEATEKSS A AAAATGRSVVGMVKDTVGS
Sbjct: 248 KVGESAFGRPPSKGVVTESEELTRRLGEEDKEATEKSSVASAAAATGRSVVGMVKDTVGS 307

Query: 392 WLGNAGEQSVPSQQSLGTSQGVEGFVDSSSSRRQAEHGGKGAEVRRLQGSAN 443
           WLG AGEQS PSQQSLGTSQGVEGFVD SSSRRQAEHGG GAEVR LQGSAN
Sbjct: 308 WLGKAGEQSAPSQQSLGTSQGVEGFVDDSSSRRQAEHGGTGAEVRVLQGSAN 359

[Close]

BLAST of CSPI03G47550 vs. ExPASy TrEMBL
Match: A0A6J1DNM6 (low-temperature-induced 65 kDa protein-like OS=Momordica charantia OX=3673 GN=LOC111022758 PE=4 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 1.4e-86
Identity = 229/412 (55.58%), Postives = 273/412 (66.26%), Query Frame = 0


Query: 4   QIPPHHHHHHHHPLGLHQSHVEGKEELQEGD-QEHHEKKSVLKKVKAKAKKIKDTITKHG 63
           QI PHHH H H             + + +GD +E HEKKSVLKKVKAKAKKIKDTITKHG
Sbjct: 5   QIAPHHHPHAH-------------QGVDQGDNEEDHEKKSVLKKVKAKAKKIKDTITKHG 64

Query: 64  HGHDHHDEEDVSDDEEDE---VVEDPEIQGAPLYEGAAMRSAVA-GKGQHQDVGIRTGTT 123
           H H HH E++  DD+EDE   VVEDP++QGAPLYEGAAMRS VA       DVG   G +
Sbjct: 65  H-HPHHYEDNEEDDDEDEDEAVVEDPQLQGAPLYEGAAMRSGVAPTPTPTPDVGF--GRS 124

Query: 124 RMMHNEPPPARREITSRPSAVDTGFTSIHNPTANYKVDDSNVAPNTTMSLSPWKLEEDPH 183
            +MH+ PPP   E TSR SAVD GF +        ++D+S VAPNTTMSLSP  LEEDPH
Sbjct: 125 TVMHHWPPPPPTETTSR-SAVDAGFATKEKEVD--RLDNSAVAPNTTMSLSPLSLEEDPH 184

Query: 184 A-PKDPHAPHISEVKVHDPSNRGSEEA-AGRSQVFDSFARMKVNDK-EPNRTGSPRGLVQ 243
           A PK PHAP  SEVK  DP+  GS+EA +G S++ DSFA+MKVND+   NR G+      
Sbjct: 185 APPKQPHAPLHSEVKASDPAAIGSQEAGSGSSEILDSFAKMKVNDENRANRAGN------ 244

Query: 244 EQGGEDQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETTEETTTNKSSSSSPLEYGK 303
            + G+ Q++Y QKISAVGSA++G A++AKDFVASKLGYG  T E    +    S  EY  
Sbjct: 245 -EAGDQQSSYGQKISAVGSALAGTAISAKDFVASKLGYGVRTGE---GEDKGVSVKEY-- 304

Query: 304 KIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRR 363
                + +KL+PGE+DRAL E ISEA+ +RK+EV           P K EVTESEELTRR
Sbjct: 305 -----LAQKLEPGEDDRALLEAISEAFQKRKEEV-----------PPKAEVTESEELTRR 364

Query: 364 LGKEDKEATEKSSAARAAAATGRSVVGMVKDTVGSWLGNAGEQSVPSQQSLG 408
           LG+ED   TE+SS A A AA  RSVV  VKDTVGSW+G  G+ S PSQQ  G
Sbjct: 365 LGREDNRETERSSTAAATAAAARSVVDTVKDTVGSWMGKGGD-SPPSQQQQG 368

[Close]

BLAST of CSPI03G47550 vs. ExPASy TrEMBL
Match: A0A6J1H2H6 (LOW QUALITY PROTEIN: low-temperature-induced 65 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111459097 PE=4 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 1.6e-85
Identity = 223/407 (54.79%), Postives = 266/407 (65.36%), Query Frame = 0


Query: 8   HHHHHHH--HPLGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKD--------T 67
           HH HHHH  H + LH+S VEG+E     D +HHEKKSVLKKVKAKAKKIKD        T
Sbjct: 11  HHAHHHHDPHNVALHRS-VEGEE-----DGQHHEKKSVLKKVKAKAKKIKDRSLSMDMAT 70

Query: 68  ITKHGHGHDHHDEEDVSDDEEDEVVEDPEIQGAPLYEGAAMRSAVAGKGQHQDVGIRTGT 127
           ITKHGHGHDHH+ ED  D+E    V DPE+QGAPLYEGA MRSA+AGKGQ QDVGI  G 
Sbjct: 71  ITKHGHGHDHHEPEDHEDEEGGGAVVDPELQGAPLYEGAVMRSAMAGKGQQQDVGI--GL 130

Query: 128 TRMMHNEPPPARREITSRPSAVDTGFTSIHNPTANYKVDDSNVAPNTTMSLSPWKLEEDP 187
              +H++PPPA RE  SRPSAV+                     PNTTMSLSPW+LEE P
Sbjct: 131 PTEVHDQPPPAPRETASRPSAVE---------------------PNTTMSLSPWRLEEGP 190

Query: 188 HAPKD----PHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARMKVNDKE-PNRTGSPRG 247
            APKD    PH PH SEVK  DP+ RG++E  G SQ FDSFARMK+N ++   R G   G
Sbjct: 191 QAPKDKAPYPHTPHNSEVKALDPAKRGNKE-TGDSQTFDSFARMKLNHEDVQTRPGLASG 250

Query: 248 LVQEQGGEDQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETTEETTTNKSSSSSPLE 307
           +  +QG  +Q++  +KIS VGS + GKA                 E T TN   SSSPLE
Sbjct: 251 I--DQG--NQSSSGEKISGVGSPMRGKA----------------EENTDTN---SSSPLE 310

Query: 308 YGKKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEEL 367
           YG+KIAL VTEKLKPGEED+ALSEVISEA+   K++VVK GE+       KG+VTESEEL
Sbjct: 311 YGRKIALAVTEKLKPGEEDKALSEVISEAYNTSKEKVVKAGEN-----QPKGKVTESEEL 359

Query: 368 TRRLGKED-KEATEK-SSAARAAAATGRSVVGMVKDTVGSWLGNAGE 398
           T+RLG ED  E T++ S A    AAT +++  MV+D+VGSW+G  G+
Sbjct: 371 TQRLGGEDNNEGTQRYSPATSTTAATVKNMADMVRDSVGSWMGKGGD 359

[Close]

BLAST of CSPI03G47550 vs. NCBI nr
Match: XP_011652891.1 (low-temperature-induced 65 kDa protein isoform X2 [Cucumis sativus])

HSP 1 Score: 830.5 bits (2144), Expect = 6.9e-237
Identity = 437/442 (98.87%), Postives = 439/442 (99.32%), Query Frame = 0


Query: 1   MDSQIPPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDTITK 60
           MDSQIPPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDTITK
Sbjct: 1   MDSQIPPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDTITK 60

Query: 61  HGHGHDHHDEEDVSDDEEDEVVEDPEIQGAPLYEGAAMRSAVAGKGQHQDVGIRTGTTRM 120
           HGHGHDHHDEEDVSDDEEDEVVEDPEIQGAPLYEGAAMRSAVAGKGQHQDVGI TGTTRM
Sbjct: 61  HGHGHDHHDEEDVSDDEEDEVVEDPEIQGAPLYEGAAMRSAVAGKGQHQDVGIGTGTTRM 120

Query: 121 MHNEPPPARREITSRPSAVDTGFTSIHNPTANYKVDDSNVAPNTTMSLSPWKLEEDPHAP 180
           MHNEPPPARREITSRPSAVDTGFTSIHN TANYKVDDSNVAPNTTMSLSPWKLE+DPHAP
Sbjct: 121 MHNEPPPARREITSRPSAVDTGFTSIHNTTANYKVDDSNVAPNTTMSLSPWKLEDDPHAP 180

Query: 181 KDPHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARMKVNDKEPNRTGSPRGLVQEQGGE 240
           KDPHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARMKV+DKEPNRTGSPRGLVQEQGGE
Sbjct: 181 KDPHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARMKVDDKEPNRTGSPRGLVQEQGGE 240

Query: 241 DQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETTEETTTNKSSSSSPLEYGKKIALT 300
           DQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETTEETTTNKSSSSSPLEYGKKIALT
Sbjct: 241 DQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETTEETTTNKSSSSSPLEYGKKIALT 300

Query: 301 VTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLGKED 360
           VTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLGKED
Sbjct: 301 VTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLGKED 360

Query: 361 KEATEKSSAARAAAATGRSVVGMVKDTVGSWLGNAGEQSVPSQQSLGTSQGVEGFVDSSS 420
           KEATEKSS ARAAAATGRSVVGMVKDTVGSWLGNAGEQSVPSQQSLGTSQGVEGFVDSSS
Sbjct: 361 KEATEKSSVARAAAATGRSVVGMVKDTVGSWLGNAGEQSVPSQQSLGTSQGVEGFVDSSS 420

Query: 421 SRRQAEHGGKGAEVRRLQGSAN 443
           SRRQAEHGGKGAEVRRLQGSAN
Sbjct: 421 SRRQAEHGGKGAEVRRLQGSAN 442

[Close]

BLAST of CSPI03G47550 vs. NCBI nr
Match: XP_031739118.1 (low-temperature-induced 65 kDa protein isoform X1 [Cucumis sativus])

HSP 1 Score: 823.9 bits (2127), Expect = 6.5e-235
Identity = 436/445 (97.98%), Postives = 439/445 (98.65%), Query Frame = 0


Query: 1   MDSQIPPHHHHHHHHPLGLHQS---HVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDT 60
           MDSQIPPHHHHHHHHPLGLHQ+   HVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDT
Sbjct: 1   MDSQIPPHHHHHHHHPLGLHQTLVGHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDT 60

Query: 61  ITKHGHGHDHHDEEDVSDDEEDEVVEDPEIQGAPLYEGAAMRSAVAGKGQHQDVGIRTGT 120
           ITKHGHGHDHHDEEDVSDDEEDEVVEDPEIQGAPLYEGAAMRSAVAGKGQHQDVGI TGT
Sbjct: 61  ITKHGHGHDHHDEEDVSDDEEDEVVEDPEIQGAPLYEGAAMRSAVAGKGQHQDVGIGTGT 120

Query: 121 TRMMHNEPPPARREITSRPSAVDTGFTSIHNPTANYKVDDSNVAPNTTMSLSPWKLEEDP 180
           TRMMHNEPPPARREITSRPSAVDTGFTSIHN TANYKVDDSNVAPNTTMSLSPWKLE+DP
Sbjct: 121 TRMMHNEPPPARREITSRPSAVDTGFTSIHNTTANYKVDDSNVAPNTTMSLSPWKLEDDP 180

Query: 181 HAPKDPHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARMKVNDKEPNRTGSPRGLVQEQ 240
           HAPKDPHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARMKV+DKEPNRTGSPRGLVQEQ
Sbjct: 181 HAPKDPHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARMKVDDKEPNRTGSPRGLVQEQ 240

Query: 241 GGEDQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETTEETTTNKSSSSSPLEYGKKI 300
           GGEDQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETTEETTTNKSSSSSPLEYGKKI
Sbjct: 241 GGEDQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETTEETTTNKSSSSSPLEYGKKI 300

Query: 301 ALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLG 360
           ALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLG
Sbjct: 301 ALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLG 360

Query: 361 KEDKEATEKSSAARAAAATGRSVVGMVKDTVGSWLGNAGEQSVPSQQSLGTSQGVEGFVD 420
           KEDKEATEKSS ARAAAATGRSVVGMVKDTVGSWLGNAGEQSVPSQQSLGTSQGVEGFVD
Sbjct: 361 KEDKEATEKSSVARAAAATGRSVVGMVKDTVGSWLGNAGEQSVPSQQSLGTSQGVEGFVD 420

Query: 421 SSSSRRQAEHGGKGAEVRRLQGSAN 443
           SSSSRRQAEHGGKGAEVRRLQGSAN
Sbjct: 421 SSSSRRQAEHGGKGAEVRRLQGSAN 445

[Close]

BLAST of CSPI03G47550 vs. NCBI nr
Match: XP_008465872.2 (PREDICTED: low-temperature-induced 65 kDa protein [Cucumis melo])

HSP 1 Score: 726.5 bits (1874), Expect = 1.4e-205
Identity = 396/443 (89.39%), Postives = 406/443 (91.65%), Query Frame = 0


Query: 1   MDSQIPPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDTITK 60
           MDSQIPP  HHHHHHPLGLHQS  EGKEEL+EG +EHHEKKSVLKKVKAKAKKIKDTITK
Sbjct: 1   MDSQIPP--HHHHHHPLGLHQS-AEGKEELEEG-EEHHEKKSVLKKVKAKAKKIKDTITK 60

Query: 61  HGHGHDHHDEEDVSDDEEDEVVEDPEIQGAPLYEGAAMRSAVAGKGQHQDVGIRTGTTRM 120
           H H HDHHDEED  DDEEDEVVEDPEIQGAPLYEGAAMRSA AGKGQHQDVGI  GTT M
Sbjct: 61  HAHAHDHHDEEDEEDDEEDEVVEDPEIQGAPLYEGAAMRSATAGKGQHQDVGIGIGTTTM 120

Query: 121 MHN-EPPPARREITSRPSAVDTGFTSIHNPTANYKVDDSNVAPNTTMSLSPWKLEEDPHA 180
           MHN  PPPARREI SRPSAVDTGFTS+ NPTAN+KVDDSNVAPNTTMSLSPWKLEEDPH 
Sbjct: 121 MHNYAPPPARREIRSRPSAVDTGFTSVENPTANHKVDDSNVAPNTTMSLSPWKLEEDPHG 180

Query: 181 PKDPHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARMKVNDKEPNRTGSPRGLVQEQGG 240
           PKDP APHISEVKVHDPSNRGSEEAAG+SQVFDSFA+MKV+DK+PNRTGSP GL QE GG
Sbjct: 181 PKDPRAPHISEVKVHDPSNRGSEEAAGKSQVFDSFAKMKVDDKKPNRTGSPHGLDQEHGG 240

Query: 241 EDQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETTEETTTNKSSSSSPLEYGKKIAL 300
           ED  NYAQKISAVGSAVSGKAVAAKDFVASKLGYGET E TT +KSSSSSPLEYGKKIAL
Sbjct: 241 EDHINYAQKISAVGSAVSGKAVAAKDFVASKLGYGETKEGTTADKSSSSSPLEYGKKIAL 300

Query: 301 TVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLGKE 360
           TVTEKLKPGEEDRALSEVISEA  RRKDEVVKVGESAFGRPPSKG VTESEELTRRLG+E
Sbjct: 301 TVTEKLKPGEEDRALSEVISEALNRRKDEVVKVGESAFGRPPSKGVVTESEELTRRLGEE 360

Query: 361 DKEATEKSSAARAAAATGRSVVGMVKDTVGSWLGNAGEQSVPSQQSLGTSQGVEGFVDSS 420
           DKEATEKSS A AAAATGRSVVGMVKDTVGSWLG AGEQS PSQQSLGTSQGVEGFVD S
Sbjct: 361 DKEATEKSSVASAAAATGRSVVGMVKDTVGSWLGKAGEQSAPSQQSLGTSQGVEGFVDDS 420

Query: 421 SSRRQAEHGGKGAEVRRLQGSAN 443
           SSRRQAEHGG GAEVR LQGSAN
Sbjct: 421 SSRRQAEHGGTGAEVRVLQGSAN 439

[Close]

BLAST of CSPI03G47550 vs. NCBI nr
Match: KAA0038494.1 (low-temperature-induced 65 kDa protein [Cucumis melo var. makuwa] >TYK31088.1 low-temperature-induced 65 kDa protein [Cucumis melo var. makuwa])

HSP 1 Score: 585.9 bits (1509), Expect = 3.0e-163
Identity = 315/352 (89.49%), Postives = 324/352 (92.05%), Query Frame = 0


Query: 92  LYEGAAMRSAVAGKGQHQDVGIRTGTTRMMHN-EPPPARREITSRPSAVDTGFTSIHNPT 151
           +YEGAAMRSA AGKGQHQDVGI  GTT MMHN  PPPARREI SRPSAVDTGFTS+ NPT
Sbjct: 8   VYEGAAMRSATAGKGQHQDVGIGIGTTTMMHNYAPPPARREIRSRPSAVDTGFTSVENPT 67

Query: 152 ANYKVDDSNVAPNTTMSLSPWKLEEDPHAPKDPHAPHISEVKVHDPSNRGSEEAAGRSQV 211
           AN+KVDDSNVAPNTTMSLSPWKLEEDPH PKDP APHISEVKVHDPSNRGSEEAAG+SQV
Sbjct: 68  ANHKVDDSNVAPNTTMSLSPWKLEEDPHGPKDPRAPHISEVKVHDPSNRGSEEAAGKSQV 127

Query: 212 FDSFARMKVNDKEPNRTGSPRGLVQEQGGEDQTNYAQKISAVGSAVSGKAVAAKDFVASK 271
           FDSFA+MKV+DK+PNRTGSP GL QE GGED  NYAQKISAVGSAVSGKAVAAKDFVASK
Sbjct: 128 FDSFAKMKVDDKKPNRTGSPHGLDQEHGGEDHINYAQKISAVGSAVSGKAVAAKDFVASK 187

Query: 272 LGYGETTEETTTNKSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVV 331
           LGYGET E TT +KSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEA  RRKDEVV
Sbjct: 188 LGYGETKEGTTADKSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEALNRRKDEVV 247

Query: 332 KVGESAFGRPPSKGEVTESEELTRRLGKEDKEATEKSSAARAAAATGRSVVGMVKDTVGS 391
           KVGESAFGRPPSKG VTESEELTRRLG+EDKEATEKSS A AAAATGRSVVGMVKDTVGS
Sbjct: 248 KVGESAFGRPPSKGVVTESEELTRRLGEEDKEATEKSSVASAAAATGRSVVGMVKDTVGS 307

Query: 392 WLGNAGEQSVPSQQSLGTSQGVEGFVDSSSSRRQAEHGGKGAEVRRLQGSAN 443
           WLG AGEQS PSQQSLGTSQGVEGFVD SSSRRQAEHGG GAEVR LQGSAN
Sbjct: 308 WLGKAGEQSAPSQQSLGTSQGVEGFVDDSSSRRQAEHGGTGAEVRVLQGSAN 359

[Close]

BLAST of CSPI03G47550 vs. NCBI nr
Match: XP_038888501.1 (low-temperature-induced 65 kDa protein isoform X1 [Benincasa hispida])

HSP 1 Score: 577.8 bits (1488), Expect = 8.1e-161
Identity = 352/456 (77.19%), Postives = 371/456 (81.36%), Query Frame = 0


Query: 1   MDSQIPPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDTITK 60
           MDSQI P  HHH HHPL LHQS VEGKE   E D + HEKKSVLKKVKAKAKKIKDTITK
Sbjct: 1   MDSQIAP--HHHLHHPLALHQS-VEGKE---EEDGQLHEKKSVLKKVKAKAKKIKDTITK 60

Query: 61  HGHG--HDHHDEEDVSD-DEEDEVVEDPEIQGAPLYEGAAMRSAVAGKGQHQDVGIRTGT 120
           HGHG  HDHHD ED  D DE+DEV+EDPEIQGAPLYEGAAMRSAVAG+GQHQDVGI  G 
Sbjct: 61  HGHGHDHDHHDYEDEDDEDEDDEVIEDPEIQGAPLYEGAAMRSAVAGQGQHQDVGI--GM 120

Query: 121 TRMMHNEPPPARREITSRPSAVDTGFTSIHNPTANY---KVDDSNVAPNTTMSLSPWKLE 180
           T  MHNEP    RE TSRPSA DTGFTS+ NPT N    KV+DS VAPNTTMSLSPWKLE
Sbjct: 121 TTGMHNEPSRGPRETTSRPSAFDTGFTSVDNPTTNKGADKVEDSAVAPNTTMSLSPWKLE 180

Query: 181 EDPHAPKDPHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARMKVNDK-EPNRTGSPRGL 240
           EDPH    PH PH S+VKVHDP+NRGSEE AG+SQVFDSFA+MKVND+ EPNR      +
Sbjct: 181 EDPHG---PHTPHNSQVKVHDPANRGSEE-AGKSQVFDSFAKMKVNDEYEPNRPSLASRI 240

Query: 241 VQEQGGEDQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETTEETTTNKSSSSSPLEY 300
             ++GGEDQTNY QK+SAVGSAVS KAVAAKDFVASKLGY ETTEET  N   SSSPLEY
Sbjct: 241 --DRGGEDQTNYGQKVSAVGSAVSEKAVAAKDFVASKLGYVETTEETINN---SSSPLEY 300

Query: 301 GKKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPP---SKGEVTESE 360
           GKKIALTVTEKLKPGEED+ALSEVISEA +RRK E+VKVGESAFGR     SKGEVTESE
Sbjct: 301 GKKIALTVTEKLKPGEEDKALSEVISEALSRRKYELVKVGESAFGRHQSLLSKGEVTESE 360

Query: 361 ELTRRLGKEDKEATEKSSAARAAAATGRSVVGMVKDTVGSWLGNAGEQSVPSQQSLGTSQ 420
           ELTRRLGKEDKEATEKSS A A AATGRSVVGMVKDTVGSWLG AGEQS PSQQSLG SQ
Sbjct: 361 ELTRRLGKEDKEATEKSSVAGATAATGRSVVGMVKDTVGSWLGKAGEQSAPSQQSLGISQ 420

Query: 421 GVEGFVDSSS----SRRQAEHGGKGAEVRRLQGSAN 443
           GVEGFVDSSS     RRQ EH G   +VR LQ SAN
Sbjct: 421 GVEGFVDSSSVGEGRRRQQEHAGTEPDVRILQDSAN 439

[Close]

BLAST of CSPI03G47550 vs. TAIR 10
Match: AT5G52300.1 (CAP160 protein )

HSP 1 Score: 101.3 bits (251), Expect = 2.1e-21
Identity = 181/646 (28.02%), Postives = 248/646 (38.39%), Query Frame = 0


Query: 1   MDSQIP-PHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEK--KSVLKKVKAKAKKIKDT 60
           M+SQ+  P+ H     P+ +H  H E        ++EHHEK    VLKKVK KAKKIK++
Sbjct: 1   MESQLTRPYGHEQAEEPIRIH--HPE--------EEEHHEKGASKVLKKVKEKAKKIKNS 60

Query: 61  ITKHGHGHDHHDEEDVSDDEEDEVVEDPEIQGAPLYEGAAMRSAVAGKGQHQDVGIRTGT 120
           +TKHG+GHDH  E+D  DDE DE  +DPE+ GAP+YE +A+R  V GK +       T  
Sbjct: 61  LTKHGNGHDHDVEDD--DDEYDE--QDPEVHGAPVYESSAVRGGVTGKPKSLSHAGETNV 120

Query: 121 TRMMHNEPP-----PARREITSRP----SAVDT--GFTSIHNPTANYKVDD---SNVAP- 180
                  PP     P      ++P    S  DT  G  ++ +P    +  D      AP 
Sbjct: 121 PASEEIVPPGTKVFPVVSSDHTKPIEPVSLQDTSYGHEALADPVRTTETSDWEAKREAPT 180

Query: 181 -----------------------NTTMSL-----------------------------SP 240
                                  NT +SL                             +P
Sbjct: 181 HYPLGVSEFSDRGESREAHQEPLNTPVSLLSATEDVTRTFAPGGEDDYLGGQRKVNVETP 240

Query: 241 WKLEEDPHAPKDPHAPHISEV-----KVHDPSNRGSEEAAGRSQVFDSFARMKVNDKEPN 300
            +LEEDP AP    + ++S V     KV DP+++G E  AG  ++ +S  RMKV D+ P+
Sbjct: 241 KRLEEDPAAP-GGGSDYLSGVSNYQSKVTDPTHKGGE--AGVPEIAESLGRMKVTDESPD 300

Query: 301 R--------------------------TGSP-----------------RGLVQEQGG--- 360
           +                            SP                 RG V+ + G   
Sbjct: 301 QKSRQGREEDFPTRSHEFDLKKESDINKNSPARFGGESKAGMEEDFPTRGDVKVESGLGR 360

Query: 361 ------EDQ---------------------------TNYAQKISAVGSAVSGKAVAAKDF 420
                  DQ                           + Y +++++  SA++ KA+AAK+ 
Sbjct: 361 DLPTGTHDQFSPELSRPKERDDSEETKDESTHETKPSTYTEQLASATSAITNKAIAAKNV 420

Query: 421 VASKLGY----GETTEETTTNKSSSSSPLEYGKKIALTV--------------------- 434
           VASKLGY    G    E+     +  S   YG+K+A TV                     
Sbjct: 421 VASKLGYTGENGGGQSESPVKDETPRSVTAYGQKVAGTVAEKLTPVYEKVKETGSTVMTK 480

[Close]

BLAST of CSPI03G47550 vs. TAIR 10
Match: AT5G52300.2 (CAP160 protein )

HSP 1 Score: 100.9 bits (250), Expect = 2.7e-21
Identity = 179/646 (27.71%), Postives = 246/646 (38.08%), Query Frame = 0


Query: 1   MDSQIP-PHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEK--KSVLKKVKAKAKKIKDT 60
           M+SQ+  P+ H     P+ +H             ++EHHEK    VLKKVK KAKKIK++
Sbjct: 1   MESQLTRPYGHEQAEEPIRIHHP-----------EEEHHEKGASKVLKKVKEKAKKIKNS 60

Query: 61  ITKHGHGHDHHDEEDVSDDEEDEVVEDPEIQGAPLYEGAAMRSAVAGKGQHQDVGIRTGT 120
           +TKHG+GHDH  E+D  DDE DE  +DPE+ GAP+YE +A+R  V GK +       T  
Sbjct: 61  LTKHGNGHDHDVEDD--DDEYDE--QDPEVHGAPVYESSAVRGGVTGKPKSLSHAGETNV 120

Query: 121 TRMMHNEPP-----PARREITSRP----SAVDT--GFTSIHNPTANYKVDD---SNVAP- 180
                  PP     P      ++P    S  DT  G  ++ +P    +  D      AP 
Sbjct: 121 PASEEIVPPGTKVFPVVSSDHTKPIEPVSLQDTSYGHEALADPVRTTETSDWEAKREAPT 180

Query: 181 -----------------------NTTMSL-----------------------------SP 240
                                  NT +SL                             +P
Sbjct: 181 HYPLGVSEFSDRGESREAHQEPLNTPVSLLSATEDVTRTFAPGGEDDYLGGQRKVNVETP 240

Query: 241 WKLEEDPHAPKDPHAPHISEV-----KVHDPSNRGSEEAAGRSQVFDSFARMKVNDKEPN 300
            +LEEDP AP    + ++S V     KV DP+++G E  AG  ++ +S  RMKV D+ P+
Sbjct: 241 KRLEEDPAAP-GGGSDYLSGVSNYQSKVTDPTHKGGE--AGVPEIAESLGRMKVTDESPD 300

Query: 301 R--------------------------TGSP-----------------RGLVQEQGG--- 360
           +                            SP                 RG V+ + G   
Sbjct: 301 QKSRQGREEDFPTRSHEFDLKKESDINKNSPARFGGESKAGMEEDFPTRGDVKVESGLGR 360

Query: 361 ------EDQ---------------------------TNYAQKISAVGSAVSGKAVAAKDF 420
                  DQ                           + Y +++++  SA++ KA+AAK+ 
Sbjct: 361 DLPTGTHDQFSPELSRPKERDDSEETKDESTHETKPSTYTEQLASATSAITNKAIAAKNV 420

Query: 421 VASKLGY----GETTEETTTNKSSSSSPLEYGKKIALTV--------------------- 434
           VASKLGY    G    E+     +  S   YG+K+A TV                     
Sbjct: 421 VASKLGYTGENGGGQSESPVKDETPRSVTAYGQKVAGTVAEKLTPVYEKVKETGSTVMTK 480

[Close]

BLAST of CSPI03G47550 vs. TAIR 10
Match: AT4G25580.1 (CAP160 protein )

HSP 1 Score: 62.8 bits (151), Expect = 8.1e-10
Identity = 161/645 (24.96%), Postives = 225/645 (34.88%), Query Frame = 0


Query: 1   MDSQIP---PHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEK--KSVLKKVKAKAKKIK 60
           MDSQ      H HH    P+ +H    EG          HHEK    VLKKVK KAKKIK
Sbjct: 1   MDSQAQLQRTHGHHQAQEPIRIHHPEEEG----------HHEKGPSKVLKKVKEKAKKIK 60

Query: 61  DTITKHGHGHDHH------DEEDVSDDEEDEVVEDPEIQGAPLYEG-------------- 120
           + +TKHGHGH+H       D+ D+  +++++   D ++ G     G              
Sbjct: 61  NVLTKHGHGHEHDRGEHIPDDHDLDQEDDEDDYNDNQLHGGAPARGKAHIPVKEEIVPPG 120

Query: 121 -----------------------AAMRSAVAGKGQHQDVGIRTGTTRMMHNEPP---PAR 180
                                   AM   +   G  +    R   T   HN P     A 
Sbjct: 121 SKAFPVVSSSHTKPSEPVRGVGHEAMSRPIKHSGVPEKEERRGAPTLTPHNTPVSLLSAT 180

Query: 181 REIT------------------SRPSAV--DTGFTSIHNPTANY--KVDD---------- 240
            ++T                   RP  +  D      H   +NY  KV D          
Sbjct: 181 EDVTRTFVPDEHKSRNQSKVNIDRPKGLDQDPAAPGSHGGLSNYQSKVTDPIGKSTGEIG 240

Query: 241 --------SNVAPNTTMSLSPWKLEEDPHAPKDPHAPHISEVKVHDPSNRGSEE-----A 300
                     +   T +      L+  P A          + KV DP+ +G+ E     A
Sbjct: 241 AASTVSAIGRLGLGTDLQKKSHGLDTKPTAQGSHGGMSSYQSKVTDPTGKGAGEQPSVAA 300

Query: 301 AGR-------------------SQVFDSFAR-------------MKVNDKEPNRTGS--- 360
            GR                   S  FD+ +R              ++  + P R+     
Sbjct: 301 FGRRNETGEDDHMGLGRDLREKSHGFDTKSRPEMGKHIPAGDYGSRLGKESPERSDEFDL 360

Query: 361 -------PRGLVQEQG--------GED-----QTNYAQKISAVGSAVSGKAVAAKDFVAS 420
                   +G+   QG        GE+     Q++Y  KIS   S V+ KAVAAK+ VAS
Sbjct: 361 GKDLPTRTQGIQNPQGFDSRGQRRGEEMHQPNQSSYTDKISLATSVVADKAVAAKNAVAS 420

Query: 421 KLGY-GETTEETTTNKSSS-SSPLEYGKKIALTV-------------------------- 443
           KLGY GE   E     + + SS   YG  +A  V                          
Sbjct: 421 KLGYSGEGGHENRVEGAENPSSAGGYGSTVASMVTPVYEKVKETGASVMTKLPFTGTGTE 480

[Close]

BLAST of CSPI03G47550 vs. TAIR 10
Match: AT5G52310.1 (low-temperature-responsive protein 78 (LTI78) / desiccation-responsive protein 29A (RD29A) )

HSP 1 Score: 46.2 bits (108), Expect = 7.9e-05
Identity = 70/263 (26.62%), Postives = 106/263 (40.30%), Query Frame = 0


Query: 16  PLGLHQSHVEGKEELQEGDQEHHEK--KSVLKKVKAKAKKIKDTITKHGHGHDH---HD- 75
           PL  HQ H E        + EHHE     + +KVKA+AKK K+++TKHG  ++H   HD 
Sbjct: 8   PLNTHQQHPE--------EVEHHENGATKMFRKVKARAKKFKNSLTKHGQSNEHEQDHDL 67

Query: 76  -EEDVSDDE-EDEVVEDPEIQGAPLYEGAAMRSAVAGKGQHQDVGIRTGTTRMMHNEPPP 135
            EED  DDE E EV++ P + G P          +   G      + +  T+   + P  
Sbjct: 68  VEEDDDDDELEPEVIDAPGVTGKPRETNVPASEEIIPPGTKVFPVVSSDYTKPTESVPVQ 127

Query: 136 ARREITSRPS-AVDTGFTS---------IHNPTANY-----KVDDSNVAPNTTMSL---- 195
                   P+ +V T FTS         IH+P +         +  + + NT +SL    
Sbjct: 128 EASYGHDAPAHSVRTTFTSDKEEKRDVPIHHPLSELSDREESRETHHESLNTPVSLLSGT 187

Query: 196 -------------------------SPWKLEEDPHAPKDPHAPHISEVKVHDPSNRGSEE 227
                                    +P  LEE+             + KV DP+    EE
Sbjct: 188 EDVTSTFAPSGDDEYLDGQRKVNVETPITLEEESAVSDYLSGVSNYQSKVTDPT---KEE 247

The following BLAST results are available for this feature:

BLAST of CSPI03G47550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q04980	2.9e-20	28.02	Low-temperature-induced 65 kDa protein OS=Arabidopsis thaliana OX=3702 GN=LTI65 ...	[more]

BLAST of CSPI03G47550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CPX7	6.8e-206	89.39	low-temperature-induced 65 kDa protein OS=Cucumis melo OX=3656 GN=LOC103503460 P...	[more]
A0A0A0LIE7	3.4e-181	98.55	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914030 PE=4 SV=1	[more]
A0A5D3E5L4	1.4e-163	89.49	Low-temperature-induced 65 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6J1DNM6	1.4e-86	55.58	low-temperature-induced 65 kDa protein-like OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1H2H6	1.6e-85	54.79	LOW QUALITY PROTEIN: low-temperature-induced 65 kDa protein-like OS=Cucurbita mo...	[more]

BLAST of CSPI03G47550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011652891.1	6.9e-237	98.87	low-temperature-induced 65 kDa protein isoform X2 [Cucumis sativus]	[more]
XP_031739118.1	6.5e-235	97.98	low-temperature-induced 65 kDa protein isoform X1 [Cucumis sativus]	[more]
XP_008465872.2	1.4e-205	89.39	PREDICTED: low-temperature-induced 65 kDa protein [Cucumis melo]	[more]
KAA0038494.1	3.0e-163	89.49	low-temperature-induced 65 kDa protein [Cucumis melo var. makuwa] >TYK31088.1 lo...	[more]
XP_038888501.1	8.1e-161	77.19	low-temperature-induced 65 kDa protein isoform X1 [Benincasa hispida]	[more]

BLAST of CSPI03G47550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52300.1	2.1e-21	28.02	CAP160 protein	[more]
AT5G52300.2	2.7e-21	27.71	CAP160 protein	[more]
AT4G25580.1	8.1e-10	24.96	CAP160 protein	[more]
AT5G52310.1	7.9e-05	26.62	low-temperature-responsive protein 78 (LTI78) / desiccation-responsive protein 2...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012418	CAP160	PFAM	PF07918	CAP160	coord: 152..177 e-value: 3.2E-7 score: 30.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 236..281
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 294..345
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 177..196
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 172..196
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 77..104
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 295..325
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 247..270
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..145
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 40..73
None	No IPR available	PANTHER	PTHR33836:SF15	SUBFAMILY NOT NAMED	coord: 10..203 coord: 199..345
IPR037491	Low-temperature-induced 78kDa/65kDa	PANTHER	PTHR33836	LOW-TEMPERATURE-INDUCED 65 KDA PROTEIN-RELATED	coord: 10..203 coord: 199..345

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G47550.1	CSPI03G47550.1	mRNA
CSPI03G47550.2	CSPI03G47550.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009737	response to abscisic acid