CSPI03G47020 (gene) Cucumber (PI 183967) v1

BLAST of CSPI03G47020 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 5.8e-239
Identity = 426/572 (74.48%), Postives = 485/572 (84.79%), Query Frame = 0

Query: 1   MATFNLSTGVPLLKSDSTRLADRLASCRIVSDAFGLEVKSGNMCLQSKQIGLVRCLRIVE 60
           MAT NL TG+ +    ++   DRL+S + V D F  + +       S QI  V+ L  + 
Sbjct: 1   MATMNLPTGLHVAAKPAS--LDRLSSAKNVGDLFFSDSRHRKRVNTSNQIMAVQSLEHIH 60

Query: 61  QREVASYNGSLDTDQDVSNSTLELQSNAFHRSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120
                     ++ +   +     + S+ +   +  +   S RKTKIVCTIGPSTSSREMI
Sbjct: 61  ---------GVNNNVYANYVNFNVPSSGYSLGQESVYLNSPRKTKIVCTIGPSTSSREMI 120

Query: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180
           WKLAE GMNVARLNMSHGDH+SHQ+TIDLVKEYNAQF DKVIAIMLDTKGPEV SGDVPK
Sbjct: 121 WKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFEDKVIAIMLDTKGPEVISGDVPK 180

Query: 181 PILLKEGQEFNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240
           PILLKEGQEFNF+IKRGVST+DTVSVNYDDF+NDVE GD LLVDGGMMSLAV+SKT D V
Sbjct: 181 PILLKEGQEFNFSIKRGVSTEDTVSVNYDDFINDVEAGDILLVDGGMMSLAVKSKTSDIV 240

Query: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300
           KC VIDGGELKSRRHLNVRGKSATLPSIT+KDW+DIKFGV+NQVDFYAVSFVKDA+VVHE
Sbjct: 241 KCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVNNQVDFYAVSFVKDAKVVHE 300

Query: 301 LKDYLKSCNADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI 360
           LKDYLKSCNADI VIVKIESADSIPNLHSI+SASDGAMVARGDLGAELPIEEVPLLQEDI
Sbjct: 301 LKDYLKSCNADIHVIVKIESADSIPNLHSIISASDGAMVARGDLGAELPIEEVPLLQEDI 360

Query: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420
           I+RC+SMQKPVIVATNMLESMIDHPTPTRAEVSDI+IAVREGADAVMLSGETAHGKYPLK
Sbjct: 361 IRRCQSMQKPVIVATNMLESMIDHPTPTRAEVSDISIAVREGADAVMLSGETAHGKYPLK 420

Query: 421 TVKVMHTVALRTESSLPINSTTPIPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRTGS 480
            VKVMH VALRTESSL  ++++P  S+ +KSHMG+MFAFH+++MANTL+TPIIVFTRTGS
Sbjct: 421 AVKVMHIVALRTESSLQKSTSSPSQSAAYKSHMGEMFAFHSSSMANTLSTPIIVFTRTGS 480

Query: 481 MAILLSHYRPGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKGH 540
           MAI+LSH RP ST+FAFT++ER+KQRL LYHGV+PIYM+FS+DAEETFSRA++ LL K  
Sbjct: 481 MAIILSHNRPSSTVFAFTNNERVKQRLALYHGVVPIYMEFSSDAEETFSRAIKLLLSKSL 540

Query: 541 VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQ 573
           V +G +VTLVQSGAQPIWR+ STHHIQVR++Q
Sbjct: 541 VKDGQYVTLVQSGAQPIWRRHSTHHIQVRKVQ 561

BLAST of CSPI03G47020 vs. ExPASy Swiss-Prot
Match: Q93Z53 (Plastidial pyruvate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP3 PE=1 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 8.2e-225
Identity = 413/565 (73.10%), Postives = 472/565 (83.54%), Query Frame = 0

Query: 11  PLLKSDSTRLADRLASCRIVSDAFGLEVKSGNMCLQSKQIGLVRCLRIVE-QREVASY-- 70
           P + S S  +   L+  R      G  V+S ++ L+   +  VR ++I E  R+  +Y  
Sbjct: 15  PQVLSSSRNIGVSLSPLR--RTLIGAGVRSTSISLRQCSLS-VRSIKISEDSRKPKAYAE 74

Query: 71  NGSLDTDQDVSNSTLELQSNAFHRSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETG 130
           NG+ D         L+  S     SRT  +  SRRKTKIVCTIGPS+SSREMIWKLAE G
Sbjct: 75  NGAFDV------GVLDSSSYRLADSRTS-SNDSRRKTKIVCTIGPSSSSREMIWKLAEAG 134

Query: 131 MNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEG 190
           MNVARLNMSHGDH+SHQ TIDLVKEYN+ F DK IAIMLDTKGPEVRSGDVP+PI L+EG
Sbjct: 135 MNVARLNMSHGDHASHQITIDLVKEYNSLFVDKAIAIMLDTKGPEVRSGDVPQPIFLEEG 194

Query: 191 QEFNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDG 250
           QEFNFTIKRGVS KDTVSVNYDDFVNDVEVGD LLVDGGMMSLAV+SKT D VKCVVIDG
Sbjct: 195 QEFNFTIKRGVSLKDTVSVNYDDFVNDVEVGDILLVDGGMMSLAVKSKTSDLVKCVVIDG 254

Query: 251 GELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHELKDYLKS 310
           GEL+SRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDA+VVHELK+YLK+
Sbjct: 255 GELQSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYLKT 314

Query: 311 CNADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDIIKRCRSM 370
           C+ADI VIVKIESADSI NL SI+SA DGAMVARGDLGAELPIEEVPLLQE+II+RCRS+
Sbjct: 315 CSADISVIVKIESADSIKNLPSIISACDGAMVARGDLGAELPIEEVPLLQEEIIRRCRSI 374

Query: 371 QKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLKTVKVMHT 430
            KPVIVATNMLESMI+HPTPTRAEVSDIAIAVREGADA+MLSGETAHGK+PLK V VMHT
Sbjct: 375 HKPVIVATNMLESMINHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVNVMHT 434

Query: 431 VALRTESSLPINSTTPIPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRTGSMAILLSH 490
           VALRTE+SLP+  T+   ++ +K HMG MFAFHA+ MANTL++P+IVFTRTGSMA+LLSH
Sbjct: 435 VALRTEASLPVR-TSASRTTAYKGHMGQMFAFHASIMANTLSSPLIVFTRTGSMAVLLSH 494

Query: 491 YRPGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKGHVVEGDHV 550
           YRP +TIFAFT+  RI QRL LY GVMPIYM+FS+DAE+T++R+L+ L D+  + EG HV
Sbjct: 495 YRPSATIFAFTNQRRIMQRLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQHV 554

Query: 551 TLVQSGAQPIWRKESTHHIQVRRIQ 573
           TLVQSG+QPIWR+ESTH IQVR+I+
Sbjct: 555 TLVQSGSQPIWREESTHLIQVRKIK 568

BLAST of CSPI03G47020 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 5.9e-207
Identity = 379/562 (67.44%), Postives = 443/562 (78.83%), Query Frame = 0

Query: 15  SDSTRLADRLASCRIVSDAFGLEVK-SGNMCLQSKQI--GLVRCLRIVEQREVASYNGSL 74
           S STR    L          G E K SG + ++S+++    VR  R VE   +      +
Sbjct: 21  SVSTRSEKLLKPASFAVKVLGNEAKRSGRVSVRSRRVVDTTVRSAR-VETEVIPVSPEDV 80

Query: 75  DTDQDVSNSTLELQSNAFHRSRTKLTTKS--RRKTKIVCTIGPSTSSREMIWKLAETGMN 134
              ++     LE+Q   F  +   + +K   RRKTKIVCT+GPST++REMIWKLAE GMN
Sbjct: 81  PNREEQLERLLEMQQ--FGDTSVGMWSKPTVRRKTKIVCTVGPSTNTREMIWKLAEAGMN 140

Query: 135 VARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQE 194
           VAR+NMSHGDH+SH+K IDLVKEYNAQ  D  IAIMLDTKGPEVRSGD+P+PI+L  GQE
Sbjct: 141 VARMNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPEVRSGDLPQPIMLDPGQE 200

Query: 195 FNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGE 254
           F FTI+RGVST   VSVNYDDFVNDVE GD LLVDGGMMS  V+SKT DSVKC V+DGGE
Sbjct: 201 FTFTIERGVSTPSCVSVNYDDFVNDVEAGDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGE 260

Query: 255 LKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHELKDYLKSCN 314
           LKSRRHLNVRGKSATLPSIT+KDWEDIKFGV+N+VDFYAVSFVKDA+VVHELK YL++  
Sbjct: 261 LKSRRHLNVRGKSATLPSITEKDWEDIKFGVENKVDFYAVSFVKDAQVVHELKKYLQNSG 320

Query: 315 ADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDIIKRCRSMQK 374
           ADI VIVKIESADSIPNLHSI++ASDGAMVARGDLGAELPIEEVP+LQE+II  CRSM K
Sbjct: 321 ADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGK 380

Query: 375 PVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLKTVKVMHTVA 434
            VIVATNMLESMI HPTPTRAEVSDIAIAVREGADAVMLSGETAHGK+PLK   VMHTVA
Sbjct: 381 AVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVA 440

Query: 435 LRTESSLPINSTTPIPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRTGSMAILLSHYR 494
           LRTE+++      P      K+HM +MFA+HAT M+NTL T  +VFTRTG MAILLSHYR
Sbjct: 441 LRTEATITSGEMPPNLGQAFKNHMSEMFAYHATMMSNTLGTSTVVFTRTGFMAILLSHYR 500

Query: 495 PGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKGHVVEGDHVTL 554
           P  TI+AFT++++I+QRL LY GV PIYM+F++DAEETF+ AL  LL +G V +G+ + +
Sbjct: 501 PSGTIYAFTNEKKIQQRLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAI 560

Query: 555 VQSGAQPIWRKESTHHIQVRRI 572
           VQSG QPIWR +STH+IQVR++
Sbjct: 561 VQSGTQPIWRSQSTHNIQVRKV 579

BLAST of CSPI03G47020 vs. ExPASy Swiss-Prot
Match: P55964 (Pyruvate kinase isozyme G, chloroplastic (Fragment) OS=Ricinus communis OX=3988 PE=2 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 9.4e-205
Identity = 360/419 (85.92%), Postives = 391/419 (93.32%), Query Frame = 0

Query: 154 NAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQEFNFTIKRGVSTKDTVSVNYDDFVN 213
           NAQ +D V++IMLDTKGPEVRSGDVP+P +LKEGQEFN TI+RGVST+DTVSVNYDDFVN
Sbjct: 1   NAQSHDNVVSIMLDTKGPEVRSGDVPQP-MLKEGQEFNPTIRRGVSTQDTVSVNYDDFVN 60

Query: 214 DVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLNVRGKSATLPSITDKDW 273
           DV VGD LLVDGGMMSLAV+SKT D VKCVV+DGGELKSRRHLNVRGKSA LPSITDKDW
Sbjct: 61  DVVVGDILLVDGGMMSLAVKSKTSDLVKCVVVDGGELKSRRHLNVRGKSARLPSITDKDW 120

Query: 274 EDIKFGVDNQVDFYAVSFVKDARVVHELKDYLKSCNADIRVIVKIESADSIPNLHSILSA 333
            DIKFGVDNQVDFYAVSFVKDA+VVHELK+YLK CNADI VIVKIESADSIPNLHSI+SA
Sbjct: 121 GDIKFGVDNQVDFYAVSFVKDAKVVHELKEYLKRCNADIHVIVKIESADSIPNLHSIISA 180

Query: 334 SDGAMVARGDLGAELPIEEVPLLQEDIIKRCRSMQKPVIVATNMLESMIDHPTPTRAEVS 393
           SDGAMVARGDLGAELPIEEVPLLQEDII+RC SMQKPVIVATNMLESMI+HPTPTRAEVS
Sbjct: 181 SDGAMVARGDLGAELPIEEVPLLQEDIIRRCHSMQKPVIVATNMLESMINHPTPTRAEVS 240

Query: 394 DIAIAVREGADAVMLSGETAHGKYPLKTVKVMHTVALRTESSLPINSTTPIPSSVHKSHM 453
           DIAIAVREGADAVMLSGETAHGKYPLK V+VMHTVALRTESS P+N TTP     +K HM
Sbjct: 241 DIAIAVREGADAVMLSGETAHGKYPLKAVRVMHTVALRTESSSPVN-TTPPAQGAYKGHM 300

Query: 454 GDMFAFHATTMANTLNTPIIVFTRTGSMAILLSHYRPGSTIFAFTDDERIKQRLVLYHGV 513
           G+MFAFHAT MANTLNTPIIVFTRTGSMA+LLSHY+P STIFAFT++ERIKQRL LY GV
Sbjct: 301 GEMFAFHATIMANTLNTPIIVFTRTGSMAVLLSHYQPASTIFAFTNEERIKQRLSLYRGV 360

Query: 514 MPIYMQFSNDAEETFSRALEFLLDKGHVVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQ 573
           MPIYM+FS+DAEETFSRAL+ LL+KG +VEG+HVTLVQSGAQPIWR+ESTHHIQVR++Q
Sbjct: 361 MPIYMEFSSDAEETFSRALQLLLNKGLLVEGEHVTLVQSGAQPIWRQESTHHIQVRKVQ 417

BLAST of CSPI03G47020 vs. ExPASy Swiss-Prot
Match: Q9LIK0 (Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP1 PE=1 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 5.3e-99
Identity = 216/498 (43.37%), Postives = 299/498 (60.04%), Query Frame = 0

Query: 83  ELQSNAFHRSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHSS 142
           EL+ N F         +S R+TK++CTIGP+T   E +  LA  GMNVARLNM HG    
Sbjct: 107 ELKENGF---------RSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDW 166

Query: 143 HQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLK--EGQEFNFTIKRGVST 202
           H+  I  V+  N +     +AIM+DT+G E+  GD+      K  +G+ + FT++   S+
Sbjct: 167 HRGVIRSVRRLNEE-KGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSS 226

Query: 203 KD--TVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLN- 262
           +   T+SV+YD F  DV VGD LLVDGGM+   V  K    VKC+  D G L  R +L  
Sbjct: 227 RPERTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTF 286

Query: 263 ------VRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHELKDYL--KSCN 322
                 VR ++A LP+I+ KDW DI FG+   VDF AVSFVK A V++ LK YL  +S  
Sbjct: 287 WRDGSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRG 346

Query: 323 ADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDIIKRCRSMQK 382
            +I VI KIES DS+ NL  I+ ASDGAMVARGDLGA++P+E+VP  Q+ I++ CR++ K
Sbjct: 347 GEIGVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNK 406

Query: 383 PVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLKTVKVMHTVA 442
           PVIVA+ +LESMI++PTPTRAEV+D++ AVR+ +DA+MLSGE+A G++P K + V+ TV+
Sbjct: 407 PVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVS 466

Query: 443 LRTE---------SSLPINSTTPIPSSVHKSHMGDMFAFHATTMANTLNT-PIIVFTRTG 502
           LR E          S+P+ +      S     + +     A  MAN L    + V+T +G
Sbjct: 467 LRIERWWREEKRHESVPLQAI----GSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSG 526

Query: 503 SMAILLSHYRPGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKG 558
            MA L+S  RP   IFAFT    +++RL L  G++P  + FS+D E   ++    L  +G
Sbjct: 527 HMASLVSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRG 586

BLAST of CSPI03G47020 vs. ExPASy TrEMBL
Match: A0A0A0LHC9 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_3G904080 PE=3 SV=1)

HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 569/573 (99.30%), Postives = 570/573 (99.48%), Query Frame = 0

Query: 1   MATFNLSTGVPLLKSDSTRLADRLASCRIVSDAFGLEVKSGNMCLQSKQIGLVRCLRIVE 60
           MATFNLSTGVPLLKSDSTRLADRLASC IVSDAFGLEVKSGNMCLQSKQIGLVRCLRIVE
Sbjct: 1   MATFNLSTGVPLLKSDSTRLADRLASCIIVSDAFGLEVKSGNMCLQSKQIGLVRCLRIVE 60

Query: 61  QREVASYNGSLDTDQDVSNSTLELQSNAFHRSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120
           QREVASYNGSLDTDQDVSNSTLELQSNAFHRSRTKLTTKSRRKTK+VCTIGPSTSSREMI
Sbjct: 61  QREVASYNGSLDTDQDVSNSTLELQSNAFHRSRTKLTTKSRRKTKVVCTIGPSTSSREMI 120

Query: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180
           WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK
Sbjct: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180

Query: 181 PILLKEGQEFNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240
           PILLKEGQEFNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV
Sbjct: 181 PILLKEGQEFNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240

Query: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300
           KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE
Sbjct: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300

Query: 301 LKDYLKSCNADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI 360
           LKDYLKSCNADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI
Sbjct: 301 LKDYLKSCNADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI 360

Query: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420
           IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK
Sbjct: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420

Query: 421 TVKVMHTVALRTESSLPINSTTPIPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRTGS 480
            VKVMHTVALRTESSLPINSTT IPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRTGS
Sbjct: 421 AVKVMHTVALRTESSLPINSTTLIPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRTGS 480

Query: 481 MAILLSHYRPGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKGH 540
           MAILLSHYRPGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKGH
Sbjct: 481 MAILLSHYRPGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKGH 540

Query: 541 VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 574
           VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG
Sbjct: 541 VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 573

BLAST of CSPI03G47020 vs. ExPASy TrEMBL
Match: A0A5A7T5H5 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G00670 PE=3 SV=1)

HSP 1 Score: 1042.7 bits (2695), Expect = 5.6e-301
Identity = 538/573 (93.89%), Postives = 542/573 (94.59%), Query Frame = 0

Query: 1   MATFNLSTGVPLLKSDSTRLADRLASCRIVSDAFGLEVKSGNMCLQSKQIGLVRCLRIVE 60
           MATFN STGVPLLKSDSTR+ADRLASCRIVSDAFGL+VKSG MCLQSKQI L        
Sbjct: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLDVKSGKMCLQSKQIAL-------- 60

Query: 61  QREVASYNGSLDTDQDVSNSTLELQSNAFHRSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120
                        DQDVSNST ELQSNAFH SRTKLTTKSRRKTKIVCTIGPSTSSREMI
Sbjct: 61  -------------DQDVSNSTFELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120

Query: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180
           WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK
Sbjct: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180

Query: 181 PILLKEGQEFNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240
           PILLKEGQEFNFTIKRGVST+DTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV
Sbjct: 181 PILLKEGQEFNFTIKRGVSTEDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240

Query: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300
           KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE
Sbjct: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300

Query: 301 LKDYLKSCNADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI 360
           LKDYLKSCNADI VIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI
Sbjct: 301 LKDYLKSCNADIHVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI 360

Query: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420
           IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK
Sbjct: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420

Query: 421 TVKVMHTVALRTESSLPINSTTPIPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRTGS 480
            VKVMHTVALRTESSLPINSTTP PSSVHKSHMGDMFAFHATTMANTLNTPIIVFTR+GS
Sbjct: 421 AVKVMHTVALRTESSLPINSTTPNPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRSGS 480

Query: 481 MAILLSHYRPGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKGH 540
           MAILLSHYRP STIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALE LLDKGH
Sbjct: 481 MAILLSHYRPCSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALELLLDKGH 540

Query: 541 VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 574
           VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG
Sbjct: 541 VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 552

BLAST of CSPI03G47020 vs. ExPASy TrEMBL
Match: A0A5D3E4W6 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004170 PE=3 SV=1)

HSP 1 Score: 1040.0 bits (2688), Expect = 3.6e-300
Identity = 536/573 (93.54%), Postives = 542/573 (94.59%), Query Frame = 0

Query: 1   MATFNLSTGVPLLKSDSTRLADRLASCRIVSDAFGLEVKSGNMCLQSKQIGLVRCLRIVE 60
           MATFN STGVPLLKSDSTR+ADRLASCRIVSDAFGL+VKSG MCLQSKQI L        
Sbjct: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLDVKSGKMCLQSKQIAL-------- 60

Query: 61  QREVASYNGSLDTDQDVSNSTLELQSNAFHRSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120
                        DQDVSNST ELQSNAFH SRTKLTTKSRRKTKIVCTIGPSTSSREMI
Sbjct: 61  -------------DQDVSNSTFELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120

Query: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180
           WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK
Sbjct: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180

Query: 181 PILLKEGQEFNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240
           PILLKEGQEFNFTI+RGVST+DTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV
Sbjct: 181 PILLKEGQEFNFTIRRGVSTEDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240

Query: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300
           KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE
Sbjct: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300

Query: 301 LKDYLKSCNADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI 360
           LKDYLKSCNADI VIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI
Sbjct: 301 LKDYLKSCNADIHVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI 360

Query: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420
           IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK
Sbjct: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420

Query: 421 TVKVMHTVALRTESSLPINSTTPIPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRTGS 480
            VKVMHTVALRTESSLPINSTTP PSSVHKSHMGDMFAFHATTMANTLNTPIIVFTR+GS
Sbjct: 421 AVKVMHTVALRTESSLPINSTTPNPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRSGS 480

Query: 481 MAILLSHYRPGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKGH 540
           MAILLSHYRP STIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRAL+ LLDKGH
Sbjct: 481 MAILLSHYRPCSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALKLLLDKGH 540

Query: 541 VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 574
           VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG
Sbjct: 541 VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 552

BLAST of CSPI03G47020 vs. ExPASy TrEMBL
Match: A0A6J1DQ72 (Pyruvate kinase OS=Momordica charantia OX=3673 GN=LOC111023306 PE=3 SV=1)

HSP 1 Score: 1013.4 bits (2619), Expect = 3.6e-292
Identity = 519/573 (90.58%), Postives = 540/573 (94.24%), Query Frame = 0

Query: 1   MATFNLSTGVPLLKSDSTRLADRLASCRIVSDAFGLEVKSGNMCLQSKQIGLVRCLRIVE 60
           MATFNLSTGVP+L+SDS R++DRLASCR V+DAF LE K+G MCLQSK I  +RC  I E
Sbjct: 1   MATFNLSTGVPILRSDSARVSDRLASCRTVADAFALEPKNGRMCLQSK-ISPIRCRSIAE 60

Query: 61  QREVASYNGSLDTDQDVSNSTLELQSNAFHRSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120
           QREV + +GSLDTDQDVSNS ++LQSN F  SRTKLTT SRRKTKIVCTIGPSTSSREMI
Sbjct: 61  QREVETCDGSLDTDQDVSNSPIKLQSNGFPLSRTKLTTNSRRKTKIVCTIGPSTSSREMI 120

Query: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180
           WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQF DKVIAIMLDTKGPEVRSGDVP+
Sbjct: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFKDKVIAIMLDTKGPEVRSGDVPQ 180

Query: 181 PILLKEGQEFNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240
           PILLKEGQEFNFTIKRGVST+DTVSVNYDDFVNDVEVGD LLVDGGMMSLAV+SKT D V
Sbjct: 181 PILLKEGQEFNFTIKRGVSTEDTVSVNYDDFVNDVEVGDILLVDGGMMSLAVKSKTKDLV 240

Query: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300
           KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE
Sbjct: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300

Query: 301 LKDYLKSCNADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI 360
           LKDYLKSCNADI VIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQE+I
Sbjct: 301 LKDYLKSCNADIYVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEEI 360

Query: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420
           IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK
Sbjct: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420

Query: 421 TVKVMHTVALRTESSLPINSTTPIPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRTGS 480
            VKVMH+VALRTESSLPI ST P   SVHKS MG+MFAFHATTMANTLNTPIIVFTRTGS
Sbjct: 421 AVKVMHSVALRTESSLPIGSTAPNQLSVHKSQMGEMFAFHATTMANTLNTPIIVFTRTGS 480

Query: 481 MAILLSHYRPGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKGH 540
           M+ILLSHYRP STIFAFTDDERIKQRLVL+HGVMPIYM+FSNDAEETFSRAL  LLDKGH
Sbjct: 481 MSILLSHYRPCSTIFAFTDDERIKQRLVLFHGVMPIYMKFSNDAEETFSRALNQLLDKGH 540

Query: 541 VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 574
           V+EGDHVTLVQSGAQPIWRKESTHHIQVRRIQG
Sbjct: 541 VIEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 572

BLAST of CSPI03G47020 vs. ExPASy TrEMBL
Match: A0A6J1GMS1 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111455395 PE=3 SV=1)

HSP 1 Score: 1010.7 bits (2612), Expect = 2.3e-291
Identity = 511/573 (89.18%), Postives = 538/573 (93.89%), Query Frame = 0

Query: 1   MATFNLSTGVPLLKSDSTRLADRLASCRIVSDAFGLEVKSGNMCLQSKQIGLVRCLRIVE 60
           MATF LSTGVPLLKSDSTR+AD LASCR VSDAFG+E K+G M +QSKQI L+RC RI E
Sbjct: 1   MATFYLSTGVPLLKSDSTRVADHLASCRNVSDAFGIEAKTGRMYVQSKQIALIRCSRIAE 60

Query: 61  QREVASYNGSLDTDQDVSNSTLELQSNAFHRSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120
           +++VASY GSLDTDQD+SNSTL+ QSN F  SR KLTTKSRRKTKIVCTIGPSTSSREMI
Sbjct: 61  KQDVASYKGSLDTDQDISNSTLDHQSNGFPLSRMKLTTKSRRKTKIVCTIGPSTSSREMI 120

Query: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180
           WKLAE GMNVARLNMSHGDHS+HQKTIDLVKEYNAQF DKVIAIMLDTKGPEVRSGDVPK
Sbjct: 121 WKLAEMGMNVARLNMSHGDHSTHQKTIDLVKEYNAQFKDKVIAIMLDTKGPEVRSGDVPK 180

Query: 181 PILLKEGQEFNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240
           PILLK+GQEFNFTIKRGVST+DTVSVNYDDF+NDVEVGD++LVDGGMMSL V+SKT+D V
Sbjct: 181 PILLKDGQEFNFTIKRGVSTEDTVSVNYDDFINDVEVGDSVLVDGGMMSLTVKSKTNDLV 240

Query: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300
           KCVV+DGGELKSRRHLNVRGKSATLPSITDKDW+DIKFGVDNQVDFYAVSFVKDARVVHE
Sbjct: 241 KCVVVDGGELKSRRHLNVRGKSATLPSITDKDWDDIKFGVDNQVDFYAVSFVKDARVVHE 300

Query: 301 LKDYLKSCNADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI 360
           LKDYLKSCNADI VIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQE I
Sbjct: 301 LKDYLKSCNADIHVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQETI 360

Query: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420
           IKRC+SMQK VIVATNMLESMIDHPTPTRAEVSDI+IAVREGADA+MLSGETAHGKYPLK
Sbjct: 361 IKRCQSMQKTVIVATNMLESMIDHPTPTRAEVSDISIAVREGADAIMLSGETAHGKYPLK 420

Query: 421 TVKVMHTVALRTESSLPINSTTPIPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRTGS 480
            V VMHTVALR ESSLP+NSTTP P SVHK HMGDM AFH TTMANTL TPIIVFTRTGS
Sbjct: 421 AVNVMHTVALRMESSLPVNSTTPNPLSVHKRHMGDMLAFHTTTMANTLKTPIIVFTRTGS 480

Query: 481 MAILLSHYRPGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKGH 540
           MAILLSHYRP STIFAFTDD+RIKQRLVLYHGVMPIYMQFS+DAEETFSRALE LLDKGH
Sbjct: 481 MAILLSHYRPCSTIFAFTDDDRIKQRLVLYHGVMPIYMQFSSDAEETFSRALELLLDKGH 540

Query: 541 VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 574
           VV+GDHVTLVQSGAQPIWRKES HHIQVRRI G
Sbjct: 541 VVDGDHVTLVQSGAQPIWRKESIHHIQVRRILG 573

BLAST of CSPI03G47020 vs. NCBI nr
Match: XP_038877819.1 (pyruvate kinase isozyme G, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1066.6 bits (2757), Expect = 7.4e-308
Identity = 544/573 (94.94%), Postives = 553/573 (96.51%), Query Frame = 0

Query: 1   MATFNLSTGVPLLKSDSTRLADRLASCRIVSDAFGLEVKSGNMCLQSKQIGLVRCLRIVE 60
           MAT NLSTGVPLLKSDSTR+ADRLASCRIVSDAFGLE KSG MCLQSKQI  VRCLRI E
Sbjct: 1   MATSNLSTGVPLLKSDSTRVADRLASCRIVSDAFGLEAKSGRMCLQSKQIASVRCLRIAE 60

Query: 61  QREVASYNGSLDTDQDVSNSTLELQSNAFHRSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120
           QRE+ASYNGSLDTDQDVSNST ELQSNAF  SRTKLTTKSRRKTKIVCTIGPST+SREMI
Sbjct: 61  QREIASYNGSLDTDQDVSNSTFELQSNAFPLSRTKLTTKSRRKTKIVCTIGPSTNSREMI 120

Query: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180
           WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQF DKVIAIMLDTKGPEVRSGDVPK
Sbjct: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFTDKVIAIMLDTKGPEVRSGDVPK 180

Query: 181 PILLKEGQEFNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240
           PILLKEGQEFNFTIKRGVST+DTVSVNYDDFVNDVEVGDTLLVDGGMMSL VQSKTDD V
Sbjct: 181 PILLKEGQEFNFTIKRGVSTEDTVSVNYDDFVNDVEVGDTLLVDGGMMSLTVQSKTDDLV 240

Query: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300
           KCVVIDGGELKSRRHLNVRGKSATLPSIT+KDWEDIKFGVDNQVDFYAVSFVKDARVVHE
Sbjct: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITEKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300

Query: 301 LKDYLKSCNADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI 360
           LKDYLKSCNADI VIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI
Sbjct: 301 LKDYLKSCNADIHVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI 360

Query: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420
           IKRCR+MQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK
Sbjct: 361 IKRCRTMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420

Query: 421 TVKVMHTVALRTESSLPINSTTPIPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRTGS 480
            VKVMHTVALRTESSLPI+ TTP P SVHKSHMGDMFAFHATTMANTL+TPIIVFTR+GS
Sbjct: 421 AVKVMHTVALRTESSLPISPTTPNPLSVHKSHMGDMFAFHATTMANTLSTPIIVFTRSGS 480

Query: 481 MAILLSHYRPGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKGH 540
           MAILLSHYRP STIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALE LLDKGH
Sbjct: 481 MAILLSHYRPCSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALELLLDKGH 540

Query: 541 VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 574
           V EGDHVTLVQSGAQPIWRKESTHHIQVRRIQG
Sbjct: 541 VAEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 573

BLAST of CSPI03G47020 vs. NCBI nr
Match: KAA0038540.1 (pyruvate kinase isozyme G [Cucumis melo var. makuwa])

HSP 1 Score: 1042.7 bits (2695), Expect = 1.1e-300
Identity = 538/573 (93.89%), Postives = 542/573 (94.59%), Query Frame = 0

Query: 1   MATFNLSTGVPLLKSDSTRLADRLASCRIVSDAFGLEVKSGNMCLQSKQIGLVRCLRIVE 60
           MATFN STGVPLLKSDSTR+ADRLASCRIVSDAFGL+VKSG MCLQSKQI L        
Sbjct: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLDVKSGKMCLQSKQIAL-------- 60

Query: 61  QREVASYNGSLDTDQDVSNSTLELQSNAFHRSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120
                        DQDVSNST ELQSNAFH SRTKLTTKSRRKTKIVCTIGPSTSSREMI
Sbjct: 61  -------------DQDVSNSTFELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120

Query: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180
           WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK
Sbjct: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180

Query: 181 PILLKEGQEFNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240
           PILLKEGQEFNFTIKRGVST+DTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV
Sbjct: 181 PILLKEGQEFNFTIKRGVSTEDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240

Query: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300
           KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE
Sbjct: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300

Query: 301 LKDYLKSCNADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI 360
           LKDYLKSCNADI VIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI
Sbjct: 301 LKDYLKSCNADIHVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI 360

Query: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420
           IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK
Sbjct: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420

Query: 421 TVKVMHTVALRTESSLPINSTTPIPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRTGS 480
            VKVMHTVALRTESSLPINSTTP PSSVHKSHMGDMFAFHATTMANTLNTPIIVFTR+GS
Sbjct: 421 AVKVMHTVALRTESSLPINSTTPNPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRSGS 480

Query: 481 MAILLSHYRPGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKGH 540
           MAILLSHYRP STIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALE LLDKGH
Sbjct: 481 MAILLSHYRPCSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALELLLDKGH 540

Query: 541 VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 574
           VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG
Sbjct: 541 VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 552

BLAST of CSPI03G47020 vs. NCBI nr
Match: TYK31137.1 (pyruvate kinase isozyme G [Cucumis melo var. makuwa])

HSP 1 Score: 1040.0 bits (2688), Expect = 7.4e-300
Identity = 536/573 (93.54%), Postives = 542/573 (94.59%), Query Frame = 0

Query: 1   MATFNLSTGVPLLKSDSTRLADRLASCRIVSDAFGLEVKSGNMCLQSKQIGLVRCLRIVE 60
           MATFN STGVPLLKSDSTR+ADRLASCRIVSDAFGL+VKSG MCLQSKQI L        
Sbjct: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLDVKSGKMCLQSKQIAL-------- 60

Query: 61  QREVASYNGSLDTDQDVSNSTLELQSNAFHRSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120
                        DQDVSNST ELQSNAFH SRTKLTTKSRRKTKIVCTIGPSTSSREMI
Sbjct: 61  -------------DQDVSNSTFELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120

Query: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180
           WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK
Sbjct: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180

Query: 181 PILLKEGQEFNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240
           PILLKEGQEFNFTI+RGVST+DTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV
Sbjct: 181 PILLKEGQEFNFTIRRGVSTEDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240

Query: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300
           KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE
Sbjct: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300

Query: 301 LKDYLKSCNADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI 360
           LKDYLKSCNADI VIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI
Sbjct: 301 LKDYLKSCNADIHVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI 360

Query: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420
           IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK
Sbjct: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420

Query: 421 TVKVMHTVALRTESSLPINSTTPIPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRTGS 480
            VKVMHTVALRTESSLPINSTTP PSSVHKSHMGDMFAFHATTMANTLNTPIIVFTR+GS
Sbjct: 421 AVKVMHTVALRTESSLPINSTTPNPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRSGS 480

Query: 481 MAILLSHYRPGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKGH 540
           MAILLSHYRP STIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRAL+ LLDKGH
Sbjct: 481 MAILLSHYRPCSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALKLLLDKGH 540

Query: 541 VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 574
           VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG
Sbjct: 541 VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 552

BLAST of CSPI03G47020 vs. NCBI nr
Match: KAG6571832.1 (hypothetical protein SDJN03_28560, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1013.4 bits (2619), Expect = 7.5e-292
Identity = 511/573 (89.18%), Postives = 539/573 (94.07%), Query Frame = 0

Query: 1   MATFNLSTGVPLLKSDSTRLADRLASCRIVSDAFGLEVKSGNMCLQSKQIGLVRCLRIVE 60
           MATF LSTGVPLLKSDSTR+ADRLASCR VSDAFG+E KSG M +QSKQI L+RC RI E
Sbjct: 1   MATFYLSTGVPLLKSDSTRVADRLASCRNVSDAFGIEAKSGRMYVQSKQIALIRCSRIAE 60

Query: 61  QREVASYNGSLDTDQDVSNSTLELQSNAFHRSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120
           +++VASY GSLDTDQD+SNST + QSN F  SR KLTTKSRRKTKIVCTIGPSTSSREMI
Sbjct: 61  KQDVASYKGSLDTDQDISNSTFDHQSNGFPLSRMKLTTKSRRKTKIVCTIGPSTSSREMI 120

Query: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180
           WKLAE GMNVARLNMSHGDHS+HQKTIDLVKEYNAQF DKVIAIMLDTKGPEVRSGDVPK
Sbjct: 121 WKLAEMGMNVARLNMSHGDHSTHQKTIDLVKEYNAQFKDKVIAIMLDTKGPEVRSGDVPK 180

Query: 181 PILLKEGQEFNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240
           PILLK+GQEFNFTIKRGVST+DTVSVNYDDF+NDVEVGD++LVDGGMMSL V+SKT+D V
Sbjct: 181 PILLKDGQEFNFTIKRGVSTEDTVSVNYDDFINDVEVGDSVLVDGGMMSLTVKSKTNDLV 240

Query: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300
           KCVV+DGGELKSRRHLNVRGKSATLPSITDKDW+DIKFGVDNQVDFYAVSFVKDARVVHE
Sbjct: 241 KCVVVDGGELKSRRHLNVRGKSATLPSITDKDWDDIKFGVDNQVDFYAVSFVKDARVVHE 300

Query: 301 LKDYLKSCNADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI 360
           LKDYLKSCNADI VIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQE I
Sbjct: 301 LKDYLKSCNADIHVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQETI 360

Query: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420
           IKRC+SMQKPVIVATNMLESMIDHPTPTRAEVSDI+IAVREGADA+MLSGETAHGKYPLK
Sbjct: 361 IKRCQSMQKPVIVATNMLESMIDHPTPTRAEVSDISIAVREGADAIMLSGETAHGKYPLK 420

Query: 421 TVKVMHTVALRTESSLPINSTTPIPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRTGS 480
            V VMHTVALR ESSLP+NS+TP P SVHK HMGDM AFH TTMANTL TPIIVFTRTGS
Sbjct: 421 AVNVMHTVALRMESSLPVNSSTPNPLSVHKRHMGDMLAFHTTTMANTLKTPIIVFTRTGS 480

Query: 481 MAILLSHYRPGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKGH 540
           MA+LLSHYRP STIFAFTDD+RIKQRLVLYHGVMPIYMQFS+DAEETFSRALE LLDKGH
Sbjct: 481 MAVLLSHYRPFSTIFAFTDDDRIKQRLVLYHGVMPIYMQFSSDAEETFSRALELLLDKGH 540

Query: 541 VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 574
           VV+GDHVTLVQSGAQPIWRKES HHIQVRRI G
Sbjct: 541 VVDGDHVTLVQSGAQPIWRKESIHHIQVRRILG 573

BLAST of CSPI03G47020 vs. NCBI nr
Match: XP_022156410.1 (pyruvate kinase isozyme G, chloroplastic [Momordica charantia])

HSP 1 Score: 1013.4 bits (2619), Expect = 7.5e-292
Identity = 519/573 (90.58%), Postives = 540/573 (94.24%), Query Frame = 0

Query: 1   MATFNLSTGVPLLKSDSTRLADRLASCRIVSDAFGLEVKSGNMCLQSKQIGLVRCLRIVE 60
           MATFNLSTGVP+L+SDS R++DRLASCR V+DAF LE K+G MCLQSK I  +RC  I E
Sbjct: 1   MATFNLSTGVPILRSDSARVSDRLASCRTVADAFALEPKNGRMCLQSK-ISPIRCRSIAE 60

Query: 61  QREVASYNGSLDTDQDVSNSTLELQSNAFHRSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120
           QREV + +GSLDTDQDVSNS ++LQSN F  SRTKLTT SRRKTKIVCTIGPSTSSREMI
Sbjct: 61  QREVETCDGSLDTDQDVSNSPIKLQSNGFPLSRTKLTTNSRRKTKIVCTIGPSTSSREMI 120

Query: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180
           WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQF DKVIAIMLDTKGPEVRSGDVP+
Sbjct: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFKDKVIAIMLDTKGPEVRSGDVPQ 180

Query: 181 PILLKEGQEFNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240
           PILLKEGQEFNFTIKRGVST+DTVSVNYDDFVNDVEVGD LLVDGGMMSLAV+SKT D V
Sbjct: 181 PILLKEGQEFNFTIKRGVSTEDTVSVNYDDFVNDVEVGDILLVDGGMMSLAVKSKTKDLV 240

Query: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300
           KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE
Sbjct: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300

Query: 301 LKDYLKSCNADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDI 360
           LKDYLKSCNADI VIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQE+I
Sbjct: 301 LKDYLKSCNADIYVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEEI 360

Query: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420
           IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK
Sbjct: 361 IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLK 420

Query: 421 TVKVMHTVALRTESSLPINSTTPIPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRTGS 480
            VKVMH+VALRTESSLPI ST P   SVHKS MG+MFAFHATTMANTLNTPIIVFTRTGS
Sbjct: 421 AVKVMHSVALRTESSLPIGSTAPNQLSVHKSQMGEMFAFHATTMANTLNTPIIVFTRTGS 480

Query: 481 MAILLSHYRPGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKGH 540
           M+ILLSHYRP STIFAFTDDERIKQRLVL+HGVMPIYM+FSNDAEETFSRAL  LLDKGH
Sbjct: 481 MSILLSHYRPCSTIFAFTDDERIKQRLVLFHGVMPIYMKFSNDAEETFSRALNQLLDKGH 540

Query: 541 VVEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 574
           V+EGDHVTLVQSGAQPIWRKESTHHIQVRRIQG
Sbjct: 541 VIEGDHVTLVQSGAQPIWRKESTHHIQVRRIQG 572

BLAST of CSPI03G47020 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 781.2 bits (2016), Expect = 5.8e-226
Identity = 413/565 (73.10%), Postives = 472/565 (83.54%), Query Frame = 0

Query: 11  PLLKSDSTRLADRLASCRIVSDAFGLEVKSGNMCLQSKQIGLVRCLRIVE-QREVASY-- 70
           P + S S  +   L+  R      G  V+S ++ L+   +  VR ++I E  R+  +Y  
Sbjct: 15  PQVLSSSRNIGVSLSPLR--RTLIGAGVRSTSISLRQCSLS-VRSIKISEDSRKPKAYAE 74

Query: 71  NGSLDTDQDVSNSTLELQSNAFHRSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETG 130
           NG+ D         L+  S     SRT  +  SRRKTKIVCTIGPS+SSREMIWKLAE G
Sbjct: 75  NGAFDV------GVLDSSSYRLADSRTS-SNDSRRKTKIVCTIGPSSSSREMIWKLAEAG 134

Query: 131 MNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEG 190
           MNVARLNMSHGDH+SHQ TIDLVKEYN+ F DK IAIMLDTKGPEVRSGDVP+PI L+EG
Sbjct: 135 MNVARLNMSHGDHASHQITIDLVKEYNSLFVDKAIAIMLDTKGPEVRSGDVPQPIFLEEG 194

Query: 191 QEFNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDG 250
           QEFNFTIKRGVS KDTVSVNYDDFVNDVEVGD LLVDGGMMSLAV+SKT D VKCVVIDG
Sbjct: 195 QEFNFTIKRGVSLKDTVSVNYDDFVNDVEVGDILLVDGGMMSLAVKSKTSDLVKCVVIDG 254

Query: 251 GELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHELKDYLKS 310
           GEL+SRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDA+VVHELK+YLK+
Sbjct: 255 GELQSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYLKT 314

Query: 311 CNADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDIIKRCRSM 370
           C+ADI VIVKIESADSI NL SI+SA DGAMVARGDLGAELPIEEVPLLQE+II+RCRS+
Sbjct: 315 CSADISVIVKIESADSIKNLPSIISACDGAMVARGDLGAELPIEEVPLLQEEIIRRCRSI 374

Query: 371 QKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLKTVKVMHT 430
            KPVIVATNMLESMI+HPTPTRAEVSDIAIAVREGADA+MLSGETAHGK+PLK V VMHT
Sbjct: 375 HKPVIVATNMLESMINHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVNVMHT 434

Query: 431 VALRTESSLPINSTTPIPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRTGSMAILLSH 490
           VALRTE+SLP+  T+   ++ +K HMG MFAFHA+ MANTL++P+IVFTRTGSMA+LLSH
Sbjct: 435 VALRTEASLPVR-TSASRTTAYKGHMGQMFAFHASIMANTLSSPLIVFTRTGSMAVLLSH 494

Query: 491 YRPGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKGHVVEGDHV 550
           YRP +TIFAFT+  RI QRL LY GVMPIYM+FS+DAE+T++R+L+ L D+  + EG HV
Sbjct: 495 YRPSATIFAFTNQRRIMQRLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQHV 554

Query: 551 TLVQSGAQPIWRKESTHHIQVRRIQ 573
           TLVQSG+QPIWR+ESTH IQVR+I+
Sbjct: 555 TLVQSGSQPIWREESTHLIQVRKIK 568

BLAST of CSPI03G47020 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 721.8 bits (1862), Expect = 4.2e-208
Identity = 379/562 (67.44%), Postives = 443/562 (78.83%), Query Frame = 0

Query: 15  SDSTRLADRLASCRIVSDAFGLEVK-SGNMCLQSKQI--GLVRCLRIVEQREVASYNGSL 74
           S STR    L          G E K SG + ++S+++    VR  R VE   +      +
Sbjct: 21  SVSTRSEKLLKPASFAVKVLGNEAKRSGRVSVRSRRVVDTTVRSAR-VETEVIPVSPEDV 80

Query: 75  DTDQDVSNSTLELQSNAFHRSRTKLTTKS--RRKTKIVCTIGPSTSSREMIWKLAETGMN 134
              ++     LE+Q   F  +   + +K   RRKTKIVCT+GPST++REMIWKLAE GMN
Sbjct: 81  PNREEQLERLLEMQQ--FGDTSVGMWSKPTVRRKTKIVCTVGPSTNTREMIWKLAEAGMN 140

Query: 135 VARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQE 194
           VAR+NMSHGDH+SH+K IDLVKEYNAQ  D  IAIMLDTKGPEVRSGD+P+PI+L  GQE
Sbjct: 141 VARMNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPEVRSGDLPQPIMLDPGQE 200

Query: 195 FNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGE 254
           F FTI+RGVST   VSVNYDDFVNDVE GD LLVDGGMMS  V+SKT DSVKC V+DGGE
Sbjct: 201 FTFTIERGVSTPSCVSVNYDDFVNDVEAGDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGE 260

Query: 255 LKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHELKDYLKSCN 314
           LKSRRHLNVRGKSATLPSIT+KDWEDIKFGV+N+VDFYAVSFVKDA+VVHELK YL++  
Sbjct: 261 LKSRRHLNVRGKSATLPSITEKDWEDIKFGVENKVDFYAVSFVKDAQVVHELKKYLQNSG 320

Query: 315 ADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDIIKRCRSMQK 374
           ADI VIVKIESADSIPNLHSI++ASDGAMVARGDLGAELPIEEVP+LQE+II  CRSM K
Sbjct: 321 ADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGK 380

Query: 375 PVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLKTVKVMHTVA 434
            VIVATNMLESMI HPTPTRAEVSDIAIAVREGADAVMLSGETAHGK+PLK   VMHTVA
Sbjct: 381 AVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVA 440

Query: 435 LRTESSLPINSTTPIPSSVHKSHMGDMFAFHATTMANTLNTPIIVFTRTGSMAILLSHYR 494
           LRTE+++      P      K+HM +MFA+HAT M+NTL T  +VFTRTG MAILLSHYR
Sbjct: 441 LRTEATITSGEMPPNLGQAFKNHMSEMFAYHATMMSNTLGTSTVVFTRTGFMAILLSHYR 500

Query: 495 PGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKGHVVEGDHVTL 554
           P  TI+AFT++++I+QRL LY GV PIYM+F++DAEETF+ AL  LL +G V +G+ + +
Sbjct: 501 PSGTIYAFTNEKKIQQRLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAI 560

Query: 555 VQSGAQPIWRKESTHHIQVRRI 572
           VQSG QPIWR +STH+IQVR++
Sbjct: 561 VQSGTQPIWRSQSTHNIQVRKV 579

BLAST of CSPI03G47020 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 363.2 bits (931), Expect = 3.8e-100
Identity = 216/498 (43.37%), Postives = 299/498 (60.04%), Query Frame = 0

Query: 83  ELQSNAFHRSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHSS 142
           EL+ N F         +S R+TK++CTIGP+T   E +  LA  GMNVARLNM HG    
Sbjct: 107 ELKENGF---------RSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDW 166

Query: 143 HQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLK--EGQEFNFTIKRGVST 202
           H+  I  V+  N +     +AIM+DT+G E+  GD+      K  +G+ + FT++   S+
Sbjct: 167 HRGVIRSVRRLNEE-KGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSS 226

Query: 203 KD--TVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLN- 262
           +   T+SV+YD F  DV VGD LLVDGGM+   V  K    VKC+  D G L  R +L  
Sbjct: 227 RPERTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTF 286

Query: 263 ------VRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHELKDYL--KSCN 322
                 VR ++A LP+I+ KDW DI FG+   VDF AVSFVK A V++ LK YL  +S  
Sbjct: 287 WRDGSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRG 346

Query: 323 ADIRVIVKIESADSIPNLHSILSASDGAMVARGDLGAELPIEEVPLLQEDIIKRCRSMQK 382
            +I VI KIES DS+ NL  I+ ASDGAMVARGDLGA++P+E+VP  Q+ I++ CR++ K
Sbjct: 347 GEIGVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNK 406

Query: 383 PVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLKTVKVMHTVA 442
           PVIVA+ +LESMI++PTPTRAEV+D++ AVR+ +DA+MLSGE+A G++P K + V+ TV+
Sbjct: 407 PVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVS 466

Query: 443 LRTE---------SSLPINSTTPIPSSVHKSHMGDMFAFHATTMANTLNT-PIIVFTRTG 502
           LR E          S+P+ +      S     + +     A  MAN L    + V+T +G
Sbjct: 467 LRIERWWREEKRHESVPLQAI----GSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSG 526

Query: 503 SMAILLSHYRPGSTIFAFTDDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLDKG 558
            MA L+S  RP   IFAFT    +++RL L  G++P  + FS+D E   ++    L  +G
Sbjct: 527 HMASLVSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRG 586

BLAST of CSPI03G47020 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 288.1 bits (736), Expect = 1.5e-77
Identity = 194/479 (40.50%), Postives = 269/479 (56.16%), Query Frame = 0

Query: 103 KTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVI 162
           KTKIVCT+GP++ S  MI KL + GMNVAR N SHG H  HQ+T+D ++   A  N  ++
Sbjct: 21  KTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLR--TAMQNTGIL 80

Query: 163 -AIMLDTKGPEVRSGDVP--KPILLKEGQEFNFTIKRGV-STKDTVSVNYDDFVNDVEVG 222
            A+MLDTKGPE+R+G +    PI LKEGQE   T    +   + T+S++Y     DV+ G
Sbjct: 81  AAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDIKGDEKTISMSYKKLPVDVKPG 140

Query: 223 DTLLVDGGMMSLAVQS--KTDDSVKCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDI 282
           +T+L   G +SLAV S      +V C   +   L  R+++N+ G    LP++TDKD EDI
Sbjct: 141 NTILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKNVNLPGVVVDLPTLTDKDVEDI 200

Query: 283 -KFGVDNQVDFYAVSFVKDARVVHELKDYLKSCNADIRVIVKIESADSIPNLHSILSASD 342
            K+GV N +D  A+SFV+    +  ++  L S +  I ++ K+E+ + + N   IL  +D
Sbjct: 201 LKWGVPNNIDMIALSFVRKGSDLVNVRKVLGSHSKSIMLMSKVENQEGVLNFDEILRETD 260

Query: 343 GAMVARGDLGAELPIEEVPLLQEDIIKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDI 402
             MVARGDLG E+PIE++ L Q+ +I +C    KPV+ AT MLESMI  P PTRAE +D+
Sbjct: 261 AFMVARGDLGMEIPIEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDV 320

Query: 403 AIAVREGADAVMLSGETAHGKYPLKTVKVMHTVALRTESSLPINS-------TTPIPSSV 462
           A AV +G D VMLSGE+A G YP   VK M  + +  ESSL  N+        TP+P S 
Sbjct: 321 ANAVLDGTDCVMLSGESAAGAYPEIAVKTMAKICIEAESSLDYNTIFKEMIRATPLPMST 380

Query: 463 HKSHMGDMFAFHATTMANTLNTP-IIVFTRTGSMAILLSHYRPGSTIFA-----FT---- 522
            +S      A  A   AN      IIV TR G+ A L++ YRP   I +     FT    
Sbjct: 381 LES-----LASSAVRTANKAKAKLIIVLTRGGTTAKLVAKYRPAVPILSVVVPVFTSDTF 440

Query: 523 ----DDERIKQRLVLYHGVMPIYMQFSNDA------EETFSRALEFLLDKGHVVEGDHV 548
                DE   +  ++Y G++P+  + S  A      EE    AL+   +KG    GD V
Sbjct: 441 NWSCSDESPARHSLIYRGLIPVLGEGSAKATDSESTEEIIESALKSATEKGLCNHGDAV 492

BLAST of CSPI03G47020 vs. TAIR 10
Match: AT5G08570.1 (Pyruvate kinase family protein )

HSP 1 Score: 287.0 bits (733), Expect = 3.4e-77
Identity = 188/463 (40.60%), Postives = 266/463 (57.45%), Query Frame = 0

Query: 103 KTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVI 162
           KTKIVCT+GP++ +  MI KL + GMNVAR N SHG H  HQ+T+D ++  +A  N  ++
Sbjct: 21  KTKIVCTLGPASRTVSMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLR--SAMHNTGIL 80

Query: 163 -AIMLDTKGPEVRSGDVP--KPILLKEGQEFNFTIKRGV-STKDTVSVNYDDFVNDVEVG 222
            A+MLDTKGPE+R+G +    PI LKEGQE   T    +   + T+S++Y     DV+ G
Sbjct: 81  AAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDIQGDESTISMSYKKLPLDVKPG 140

Query: 223 DTLLVDGGMMSLAVQSKTDDS--VKCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDI 282
           +T+L   G +SLAV S   +S  V+C   +   L  R+++N+ G    LP++TDKD EDI
Sbjct: 141 NTILCADGSISLAVLSCDPESGTVRCRCENSAMLGERKNVNLPGVVVDLPTLTDKDIEDI 200

Query: 283 -KFGVDNQVDFYAVSFVKDARVVHELKDYLKSCNADIRVIVKIESADSIPNLHSILSASD 342
             +GV N +D  A+SFV+    +  ++  L S    I ++ K+E+ + + N   IL  +D
Sbjct: 201 LGWGVPNSIDMIALSFVRKGSDLVNVRKVLGSHAKSIMLMSKVENQEGVINFDEILRETD 260

Query: 343 GAMVARGDLGAELPIEEVPLLQEDIIKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDI 402
             MVARGDLG E+PIE++ L Q+ +I +C    KPV+ AT MLESMI  P PTRAE +D+
Sbjct: 261 AFMVARGDLGMEIPIEKIFLAQKLMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDV 320

Query: 403 AIAVREGADAVMLSGETAHGKYPLKTVKVMHTVALRTESSLPINS-------TTPIPSSV 462
           A AV +G D VMLSGE+A G YP   VKVM  + +  ESSL  N+        TP+P S 
Sbjct: 321 ANAVLDGTDCVMLSGESAAGAYPEIAVKVMAKICIEAESSLDYNTIFKEMIRATPLPMSP 380

Query: 463 HKSHMGDMFAFHATTMANTLNTP-IIVFTRTGSMAILLSHYRPGSTIFAFT--------- 522
            +S      A  A   AN      IIV TR GS A L++ YRP   I +           
Sbjct: 381 LES-----LASSAVRTANKARAKLIIVLTRGGSTANLVAKYRPAVPILSVVVPVMTTDSF 440

Query: 523 ----DDERIKQRLVLYHGVMPIYMQFSNDAEETFSRALEFLLD 538
                DE   +  ++Y G++P+  + S  A+ T S A E +++
Sbjct: 441 DWSCSDESPARHSLIYRGLIPMLAEGS--AKATDSEATEVIIE 474