Homology
BLAST of CSPI03G46970 vs. ExPASy Swiss-Prot
Match:
B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)
HSP 1 Score: 410.2 bits (1053), Expect = 3.2e-113
Identity = 211/489 (43.15%), Postives = 326/489 (66.67%), Query Frame = 0
Query: 7 VCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNKAFRVVG 66
VC I G F R N +Y KL RK + LPPGD+GWP +G L+F K F G
Sbjct: 15 VCYIFG------SKFVRNLNGWYYDVKLRRKEH-PLPPGDMGWPLIGDLLSFIKDFS-SG 74
Query: 67 DPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKSVKILET 126
P +FI+ +L+YGR G+YK+HLFG P++IV P++CRR+ DD F+ YPKS+K L
Sbjct: 75 HPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELAR 134
Query: 127 NGNFLKI---EHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEVMDK 186
+ + EH++ ++I SP+ G + L+ ++E +E++V LEE SSM + P+E++ +
Sbjct: 135 CRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSM-KHPVELLKE 194
Query: 187 IGGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKARNKI 246
+ + FK ++H+F+G+ D + ++ + + +L + S +P ++PGFT+ +AL+AR K+
Sbjct: 195 MKKVSFKAIVHVFMGSSNQD-IIKKIGSSFTDLYNGMFS-IPINVPGFTFHKALEARKKL 254
Query: 247 EKILRCVIEKKRKRFEK-DDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFFAG 306
KI++ V++++R E ++ L+D L+ K+ENG K D+ I DLL+G FAG
Sbjct: 255 AKIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRK-LEDEDISDLLIGLLFAG 314
Query: 307 HNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFINE 366
H + AT+ MW++ +++Q+PHI + AKEEQE I R R S+QK L+ +EIKQM +L++ I+E
Sbjct: 315 HESTATSLMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQVIDE 374
Query: 367 VLRRNTVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWDNY 426
LR +A FR+A +VNINGY IPKGW V IW+ A+HMDS+ Y NP++F+PSRWD+Y
Sbjct: 375 TLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRWDDY 434
Query: 427 TPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPMGL 486
K G F+PFG GS+ CPG++LAKLE++I LH+F+ NY++E ++ C+ T LP +KP
Sbjct: 435 NAKAGTFLPFGAGSRLCPGADLAKLEISIFLHYFLRNYRLERINPECHVTSLPVSKPTDN 491
Query: 487 C--RIVRVS 490
C ++++VS
Sbjct: 495 CLAKVIKVS 491
BLAST of CSPI03G46970 vs. ExPASy Swiss-Prot
Match:
Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)
HSP 1 Score: 382.5 bits (981), Expect = 7.2e-105
Identity = 209/482 (43.36%), Postives = 301/482 (62.45%), Query Frame = 0
Query: 13 VVLGFFYV--FARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNKAFRVVGDPYT 72
++LG F + +R N Y KLG K + LPPGDLGWP +G+ +F +AF+ DP +
Sbjct: 14 IILGLFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFK-TSDPES 73
Query: 73 FIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKSVKILETNGNF 132
FI +++ RYGR G+YK+H+FG P V+VT PE CRR+ DD+ F +PKS L +F
Sbjct: 74 FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 133
Query: 133 LKI---EHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEVMDKIGGL 192
+ I EHK ++ ++P+NG E LS +++FIE+ V LE+WS M IE + + L
Sbjct: 134 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE--IEFLSHLRKL 193
Query: 193 FFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKARNKIEKIL 252
FKV+++IFL +E + M L Y L + + + +LPGF Y RALKAR K+
Sbjct: 194 TFKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRA-MGINLPGFAYHRALKARKKLVAAF 253
Query: 253 RCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFFAGHNTPA 312
+ ++ +R + K + ++ +LD LI K+ENG + D+ IIDLLL AGH +
Sbjct: 254 QSIVTNRRNQ-RKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSG 313
Query: 313 TAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFINEVLRRN 372
MWA + + ++P I Q AKEEQE I+++R QK LT +E ++M +L++ I+E LR
Sbjct: 314 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 373
Query: 373 TVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWDNYTPKPG 432
T + T FR+AK++V ++GY IPKGW V W VH+D +IY +P+ FDPSRW+ YTPK G
Sbjct: 374 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 433
Query: 433 EFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPMGLC--RI 488
F+PFGLGS CPG++LAKLE++I LHHF+L Y+VE + C LP +P C RI
Sbjct: 434 TFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 486
BLAST of CSPI03G46970 vs. ExPASy Swiss-Prot
Match:
Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)
HSP 1 Score: 364.0 bits (933), Expect = 2.7e-99
Identity = 184/483 (38.10%), Postives = 294/483 (60.87%), Query Frame = 0
Query: 4 IMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNKAFR 63
++ V+ GVVL RR ++ LG + LPPG++GWP VGS AF +AF+
Sbjct: 19 VLAAAVVAGVVL--VDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFK 78
Query: 64 VVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKSVKI 123
G+P FI + + R+GR G+Y++ +F PT++ PE C+++ +DDE F +PK+
Sbjct: 79 -SGNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVT 138
Query: 124 LETNGNFLKI---EHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEV 183
L +F+ + +H+ K+ A+P+NG + L+ ++ FI+Q V L WSS +E
Sbjct: 139 LIGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEF 198
Query: 184 MDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKAR 243
+ ++ + FK+++ IF+ DD M L Y +L M + +LPGF Y RAL+AR
Sbjct: 199 LTELRRMTFKIIVQIFMSG-ADDATMEALERSYTDLNYG-MRAMAINLPGFAYYRALRAR 258
Query: 244 NKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFF 303
K+ +L+ V++ +R K + ++D+LI A++E G + +DD I+D+L+
Sbjct: 259 RKLVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERG-RRLADDEIVDVLIMYLN 318
Query: 304 AGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFI 363
AGH + MWA + + +NP IF AK EQE I+R P+TQ GLT ++ K+M FL++ +
Sbjct: 319 AGHESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVV 378
Query: 364 NEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWD 423
+E LR ++ +FR+A ++ +NGY IPKGW VQ+W +VHMD Q+Y +P+ F+PSRW+
Sbjct: 379 DETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWE 438
Query: 424 NYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPM 483
PK G F+PFGLG++ CPG++LAKLE+++ LHHF+L YK++ + C LP +P+
Sbjct: 439 GPPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLLGYKLKRANPKCRVRYLPHPRPV 495
BLAST of CSPI03G46970 vs. ExPASy Swiss-Prot
Match:
O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)
HSP 1 Score: 363.6 bits (932), Expect = 3.5e-99
Identity = 193/483 (39.96%), Postives = 298/483 (61.70%), Query Frame = 0
Query: 13 VVLGFFYV--FARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNKAFRVVGDPYT 72
+VLG F + R+ N Y LG + LPPGDLGWPF+G+ L+F +AF+ DP +
Sbjct: 15 MVLGCFGLNWLVRKVNVWLYESSLGENRH-YLPPGDLGWPFIGNMLSFLRAFK-TSDPDS 74
Query: 73 FIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKSVKILETNGNF 132
F T + RYG G+YK+H+FG P++IVT + CRR+ DD+ F+P +P S L +F
Sbjct: 75 FTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSF 134
Query: 133 LKI---EHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEVMDKIGGL 192
+ I EHK ++ A+P+NG E LS ++ +IE+ V L++W+ M E + + L
Sbjct: 135 VGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE--FEFLTHLRKL 194
Query: 193 FFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKARNKIEKIL 252
F+++++IFL +E + M L Y L + + + ++PGF Y RALKAR +
Sbjct: 195 TFRIIMYIFLSSE-SENVMDALEREYTALNYGVRA-MAVNIPGFAYHRALKARKTLVAAF 254
Query: 253 RCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFFAGHNTPA 312
+ ++ ++R + K + + +LD L+ K+E+G K+ D+ IID+LL AGH +
Sbjct: 255 QSIVTERRNQ-RKQNILSNKKDMLDNLLNVKDEDG-KTLDDEEIIDVLLMYLNAGHESSG 314
Query: 313 TAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFINEVLRRN 372
MWA + + ++P + Q AK EQE I++ RP QKGL+ +E ++M+FL++ ++E LR
Sbjct: 315 HTIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVI 374
Query: 373 TVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWDN-YTPKP 432
T + T FR+AKT+V +NGY IPKGW V W VH+D +++ +P+ FDP+RWDN + PK
Sbjct: 375 TFSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKA 434
Query: 433 GEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPMGLCRIV 490
G F+PFG GS CPG++LAKLE++I LHHF+L Y+V+ + C LP +P C +
Sbjct: 435 GAFLPFGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNC-LA 488
BLAST of CSPI03G46970 vs. ExPASy Swiss-Prot
Match:
Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)
HSP 1 Score: 356.7 bits (914), Expect = 4.3e-97
Identity = 183/476 (38.45%), Postives = 289/476 (60.71%), Query Frame = 0
Query: 22 ARRFNEIWY-SFKLGRKIYKSLPPGDLGWPFVGSTLAFNKAFRVVGDPYTFIHTHLLRYG 81
A R WY + LG LPPGD+GWP VG AF +AF+ G P +FI + R+G
Sbjct: 24 AVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFK-SGRPDSFIDSFARRFG 83
Query: 82 RVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKSVKILETNGNFLKI---EHKI 141
R G+Y++ +F PT++ T PE C+++ +DD+ F +PK+ L +F+ + EH+
Sbjct: 84 RAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVALIGPKSFVNMGYDEHRR 143
Query: 142 GYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRRE-PIEVMDKIGGLFFKVVLHIF 201
K+ A+P+NG + L+ ++ FI+ V L WS + E + ++ + F++++ IF
Sbjct: 144 LRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLTELRRMTFRIIVQIF 203
Query: 202 LGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKARNKIEKILRCVIEKKRK 261
+G D++ AEL Y EL M + DLPGF Y +A++AR ++ L+ V++++R
Sbjct: 204 MGG-ADERTAAELERTYTELNYG-MRAMAIDLPGFAYHKAIRARRRLVAALQRVLDERRA 263
Query: 262 RFEKD-DGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFFAGHNTPATAAMWALL 321
R K G ++D+LI ++E G + DD IID+L+ AGH + MWA +
Sbjct: 264 RGGKTAAGAAAPVDMMDRLIAVEDE-GGRRLQDDEIIDVLVMYLNAGHESSGHITMWATV 323
Query: 322 HISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFINEVLRRNTVAPTNFR 381
+ +NP I AK EQE+I+R P QKGLT ++ ++M +L++ ++E LR ++ +FR
Sbjct: 324 FLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYLSQVVDETLRFVNISFVSFR 383
Query: 382 KAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWDNYTPKPGEFIPFGLG 441
+A +V +NGY IPKGW VQ+W +VHMD Q+Y +P+ FDPSRW+ P+ G F+PFGLG
Sbjct: 384 QATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDPSRWEGPPPRAGTFLPFGLG 443
Query: 442 SKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPMGLC--RIVRVS 490
++ CPG++LAKLE+++ LHHF+L YK+ + NC LP +P+ C +I R+S
Sbjct: 444 TRLCPGNDLAKLEISVFLHHFLLGYKLTRKNPNCRVRYLPHPRPVDNCLAKITRLS 495
BLAST of CSPI03G46970 vs. ExPASy TrEMBL
Match:
A0A0A0LHC2 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa3G9038540 PE=2 SV=1)
HSP 1 Score: 930.2 bits (2403), Expect = 3.4e-267
Identity = 462/490 (94.29%), Postives = 463/490 (94.49%), Query Frame = 0
Query: 1 MELIMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNK 60
MELIMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNK
Sbjct: 1 MELIMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNK 60
Query: 61 AFRVVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKS 120
AFRVVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKS
Sbjct: 61 AFRVVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKS 120
Query: 121 VKILETNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEV 180
VKILETNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEV
Sbjct: 121 VKILETNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEV 180
Query: 181 MDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKAR 240
MDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLY+ELGLVIMSFLPYDLPGFTYRRALKAR
Sbjct: 181 MDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLYKELGLVIMSFLPYDLPGFTYRRALKAR 240
Query: 241 NKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFF 300
NKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFF
Sbjct: 241 NKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFF 300
Query: 301 AGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFI 360
AGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFI
Sbjct: 301 AGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFI 360
Query: 361 NEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWD 420
NEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWT
Sbjct: 361 NEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWT--------------------------- 420
Query: 421 NYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPM 480
NYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPM
Sbjct: 421 NYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPM 463
Query: 481 GLCRIVRVSQ 491
GLCRIVRVSQ
Sbjct: 481 GLCRIVRVSQ 463
BLAST of CSPI03G46970 vs. ExPASy TrEMBL
Match:
A0A0A0LF52 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G903530 PE=3 SV=1)
HSP 1 Score: 671.8 bits (1732), Expect = 2.2e-189
Identity = 334/385 (86.75%), Postives = 356/385 (92.47%), Query Frame = 0
Query: 4 IMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNKAFR 63
+MNVCVIIGVVLG FYVF RRFNEIWY FKLGRK+ KSLPPGDLGWPFVGST +F KAF+
Sbjct: 3 LMNVCVIIGVVLG-FYVFVRRFNEIWYLFKLGRKLNKSLPPGDLGWPFVGSTFSFYKAFK 62
Query: 64 VVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKSVKI 123
V GDPYTFIHT LLRYGRVGMYKSHL+GMPT+IVTNPEICRRIYLDDE FEPNYPKSVKI
Sbjct: 63 VEGDPYTFIHTLLLRYGRVGMYKSHLYGMPTIIVTNPEICRRIYLDDERFEPNYPKSVKI 122
Query: 124 LETNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEVMDK 183
LETNG+F KI+HK GYKI+ASPMNGSEVLSKHVEFIEQ VE GLEEWSSMRREPIE++D+
Sbjct: 123 LETNGDFSKIDHKSGYKIMASPMNGSEVLSKHVEFIEQTVEKGLEEWSSMRREPIELVDE 182
Query: 184 IGGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKARNKI 243
IGGL FK++LHIFLGNEID QAMAELHTLY+ELGLVIMSFLPYDLPGFTYRRALKAR KI
Sbjct: 183 IGGLLFKIILHIFLGNEIDGQAMAELHTLYKELGLVIMSFLPYDLPGFTYRRALKARKKI 242
Query: 244 EKILRCVIEKKRKRFEKDDGTTE-VYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFFAG 303
EKIL CVIEKKRKRFEKDDGT E V+ +DKLIVA NENGSK Y++ TIIDL+LG FFAG
Sbjct: 243 EKILHCVIEKKRKRFEKDDGTNEVVHCQVDKLIVATNENGSKPYNNSTIIDLILGIFFAG 302
Query: 304 HNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFINE 363
HNTPA AAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMK+LTKFINE
Sbjct: 303 HNTPAIAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKYLTKFINE 362
Query: 364 VLRRNTVAPTNFRKAKTNVNINGYT 388
VLRRNTVAPTNFRKA+T VNIN T
Sbjct: 363 VLRRNTVAPTNFRKARTYVNINEST 386
BLAST of CSPI03G46970 vs. ExPASy TrEMBL
Match:
A0A097IYP6 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa3G903550 PE=2 SV=1)
HSP 1 Score: 665.2 bits (1715), Expect = 2.1e-187
Identity = 325/492 (66.06%), Postives = 403/492 (81.91%), Query Frame = 0
Query: 1 MELIMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNK 60
ME+I +VCVIIGVVLG + RR NE+WY KLG + YKSLPPGDLGWP +G + + K
Sbjct: 1 MEMI-SVCVIIGVVLG-VCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYK 60
Query: 61 AFRVVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKS 120
F V DP +FI + RYG+ GMYK+HL+G PTVIVTNPEICRRIYLD+ F+ +YPKS
Sbjct: 61 TFIVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKS 120
Query: 121 VKILE-TNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIE 180
VKILE ++G+F ++HKI YK++ASPMNG EVLS +V+FIE+++ GLEEWSSM REPI+
Sbjct: 121 VKILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIK 180
Query: 181 VMDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGL-VIMSFLPYDLPGFTYRRALK 240
++D+IG LFFKV+ IFLG+++D + M ELHTLY+EL ++MS PYD PGFT+ + LK
Sbjct: 181 LVDEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLK 240
Query: 241 ARNKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGT 300
AR KIE ++ V+E+K +RFE +D T+EV +DKL+VA NENG+K YS++ I DL+LG
Sbjct: 241 ARRKIENTIQGVVEEKIRRFE-NDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGI 300
Query: 301 FFAGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTK 360
FFAGH+TPA AA WALLHISQNPH+FQ AKEEQESIIRQRPS QKGLT EIKQMK+LTK
Sbjct: 301 FFAGHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTK 360
Query: 361 FINEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSR 420
INEVLRRNT+ TNFR+ KT+VNING+ IPKGWTVQIW++AVHMD QIYSNPQ+F+PSR
Sbjct: 361 IINEVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSR 420
Query: 421 WDNYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAK 480
WDNYTPKPGEFIPFGLGS+FCPGSELAKLE+TILLHHF+LNYK+E V+ N+ ++PS +
Sbjct: 421 WDNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPR 480
Query: 481 PMG-LCRIVRVS 490
P LC+I+R+S
Sbjct: 481 PENCLCKIIRLS 489
BLAST of CSPI03G46970 vs. ExPASy TrEMBL
Match:
A0A6J1GLR1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 SV=1)
HSP 1 Score: 662.1 bits (1707), Expect = 1.7e-186
Identity = 318/479 (66.39%), Postives = 388/479 (81.00%), Query Frame = 0
Query: 5 MNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNKAFRV 64
+NVCVIIG VLG Y+F R+ NEIWY KLGRK YKSLPPGD+GWP +GS+ +F AF V
Sbjct: 4 INVCVIIGFVLG-VYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTV 63
Query: 65 VGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKSVKIL 124
GDP +FI+ L R GRVGMYKSH +G PT+IVT+PEICRRIYLDDE F+PNYPKSVKIL
Sbjct: 64 GGDPDSFIYHLLSRNGRVGMYKSHFYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVKIL 123
Query: 125 ETNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEVMDKI 184
E NG F + +HK Y+I+A+PMNG EVLS +V+FI+Q++ GLEEWSSM +EPIE++ +I
Sbjct: 124 EVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSM-KEPIELLGEI 183
Query: 185 GGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKARNKIE 244
G LFFKV+ IFLG +I MAEL TLY++LG I+S LPYDLPG T+ ALKAR KIE
Sbjct: 184 GSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKIE 243
Query: 245 KILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFFAGHN 304
IL+CVIE+KR+ EK++ EV S +DKLI+A++ENG K Y ++ IIDLLLG AGH+
Sbjct: 244 NILQCVIEEKRRALEKEE--REVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHH 303
Query: 305 TPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFINEVL 364
TPA AAMWAL+HIS+ PH+F AKEEQESII+QRPSTQKGLTFQEIKQMK+L KFINE+L
Sbjct: 304 TPAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKFINELL 363
Query: 365 RRNTVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWDNYTP 424
RRNT+A +FRKAK +VNIN Y IPKGW V IW+ A+HMD Q+YSNPQ+FDPSRW+NY+P
Sbjct: 364 RRNTIAVASFRKAKKDVNINDYIIPKGWNVLIWTRAIHMDPQLYSNPQEFDPSRWENYSP 423
Query: 425 KPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPMGLC 484
KPG F+PFG+G++FCPGSEL KLE+TILLHHF+LNYK+E V+ C T LP+ K + C
Sbjct: 424 KPGTFLPFGIGTRFCPGSELTKLEITILLHHFILNYKMERVNPKCCITHLPAPKLVDNC 478
BLAST of CSPI03G46970 vs. ExPASy TrEMBL
Match:
A0A6J1GJM1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 SV=1)
HSP 1 Score: 662.1 bits (1707), Expect = 1.7e-186
Identity = 318/483 (65.84%), Postives = 392/483 (81.16%), Query Frame = 0
Query: 1 MELIMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNK 60
ME+I NVC IIG VLG Y+F R+ NEIWY KLGRK YKSLPPGD+GWP +GS+ +F
Sbjct: 1 MEMI-NVCTIIGAVLG-VYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYN 60
Query: 61 AFRVVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKS 120
AF V GDP +FI+ L R GRVGMYKSHL+G PT+IVT+PEICRRIYLDDE F+PNYPKS
Sbjct: 61 AFTVGGDPDSFIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKS 120
Query: 121 VKILETNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEV 180
VKILE NG F + +HK Y+I+A+PMNG EVLS +V+FI+Q++ GLEEWSSM +EPIE+
Sbjct: 121 VKILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSM-KEPIEL 180
Query: 181 MDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKAR 240
+ +IG LFFKV+ IFLG +I MAEL TLY++LG I+S LPYD+PG Y RALKAR
Sbjct: 181 LGEIGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLVYHRALKAR 240
Query: 241 NKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFF 300
KIE +L CVIE+KR+ E+ + T EV+S +DKLI+A++ENG K Y ++TIIDLLLG
Sbjct: 241 KKIENVLECVIEEKRRALEQKE-TREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLLH 300
Query: 301 AGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFI 360
AGH+TPA AAMWAL+HIS+ PHIF AKEEQESI++QRPSTQKGLTFQEIKQMK+L KFI
Sbjct: 301 AGHHTPAYAAMWALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKFI 360
Query: 361 NEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWD 420
NE+LRRNT+ +FRKAKT++NIN Y IPKGW+V IW+ AVHMD Q+YSNP++FDPSRW+
Sbjct: 361 NELLRRNTIVVASFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRWE 420
Query: 421 NYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPM 480
N++PKPG FIPFG+G++FCPGSEL KLE+TILLHHF+LNYK+E + C + LP+ K +
Sbjct: 421 NHSPKPGTFIPFGIGTRFCPGSELTKLEITILLHHFILNYKMERTNPKCCISHLPAPKLV 479
Query: 481 GLC 484
C
Sbjct: 481 DNC 479
BLAST of CSPI03G46970 vs. NCBI nr
Match:
KAE8651450.1 (hypothetical protein Csa_001467 [Cucumis sativus])
HSP 1 Score: 1005.4 bits (2598), Expect = 1.7e-289
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0
Query: 1 MELIMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNK 60
MELIMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNK
Sbjct: 1 MELIMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNK 60
Query: 61 AFRVVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKS 120
AFRVVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKS
Sbjct: 61 AFRVVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKS 120
Query: 121 VKILETNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEV 180
VKILETNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEV
Sbjct: 121 VKILETNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEV 180
Query: 181 MDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKAR 240
MDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKAR
Sbjct: 181 MDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKAR 240
Query: 241 NKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFF 300
NKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFF
Sbjct: 241 NKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFF 300
Query: 301 AGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFI 360
AGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFI
Sbjct: 301 AGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFI 360
Query: 361 NEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWD 420
NEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWD
Sbjct: 361 NEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWD 420
Query: 421 NYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPM 480
NYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPM
Sbjct: 421 NYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPM 480
Query: 481 GLCRIVRVSQ 491
GLCRIVRVSQ
Sbjct: 481 GLCRIVRVSQ 490
BLAST of CSPI03G46970 vs. NCBI nr
Match:
XP_004148514.3 (beta-amyrin 11-oxidase-like isoform X1 [Cucumis sativus])
HSP 1 Score: 1005.4 bits (2598), Expect = 1.7e-289
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0
Query: 1 MELIMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNK 60
MELIMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNK
Sbjct: 1 MELIMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNK 60
Query: 61 AFRVVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKS 120
AFRVVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKS
Sbjct: 61 AFRVVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKS 120
Query: 121 VKILETNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEV 180
VKILETNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEV
Sbjct: 121 VKILETNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEV 180
Query: 181 MDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKAR 240
MDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKAR
Sbjct: 181 MDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKAR 240
Query: 241 NKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFF 300
NKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFF
Sbjct: 241 NKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFF 300
Query: 301 AGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFI 360
AGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFI
Sbjct: 301 AGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFI 360
Query: 361 NEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWD 420
NEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWD
Sbjct: 361 NEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWD 420
Query: 421 NYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPM 480
NYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPM
Sbjct: 421 NYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPM 480
Query: 481 GLCRIVRVSQ 491
GLCRIVRVSQ
Sbjct: 481 GLCRIVRVSQ 490
BLAST of CSPI03G46970 vs. NCBI nr
Match:
NP_001295866.1 (beta-amyrin 11-oxidase-like [Cucumis sativus] >AIT72035.1 cytochrome P450 [Cucumis sativus])
HSP 1 Score: 930.2 bits (2403), Expect = 7.1e-267
Identity = 462/490 (94.29%), Postives = 463/490 (94.49%), Query Frame = 0
Query: 1 MELIMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNK 60
MELIMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNK
Sbjct: 1 MELIMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNK 60
Query: 61 AFRVVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKS 120
AFRVVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKS
Sbjct: 61 AFRVVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKS 120
Query: 121 VKILETNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEV 180
VKILETNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEV
Sbjct: 121 VKILETNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEV 180
Query: 181 MDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKAR 240
MDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLY+ELGLVIMSFLPYDLPGFTYRRALKAR
Sbjct: 181 MDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLYKELGLVIMSFLPYDLPGFTYRRALKAR 240
Query: 241 NKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFF 300
NKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFF
Sbjct: 241 NKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFF 300
Query: 301 AGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFI 360
AGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFI
Sbjct: 301 AGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFI 360
Query: 361 NEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWD 420
NEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWT
Sbjct: 361 NEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWT--------------------------- 420
Query: 421 NYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPM 480
NYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPM
Sbjct: 421 NYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPM 463
Query: 481 GLCRIVRVSQ 491
GLCRIVRVSQ
Sbjct: 481 GLCRIVRVSQ 463
BLAST of CSPI03G46970 vs. NCBI nr
Match:
XP_011652660.1 (beta-amyrin 11-oxidase [Cucumis sativus])
HSP 1 Score: 865.9 bits (2236), Expect = 1.6e-247
Identity = 422/489 (86.30%), Postives = 452/489 (92.43%), Query Frame = 0
Query: 4 IMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNKAFR 63
+MNVCVIIGVVLG FYVF RRFNEIWY FKLGRK+ KSLPPGDLGWPFVGST +F KAF+
Sbjct: 3 LMNVCVIIGVVLG-FYVFVRRFNEIWYLFKLGRKLNKSLPPGDLGWPFVGSTFSFYKAFK 62
Query: 64 VVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKSVKI 123
V GDPYTFIHT LLRYGRVGMYKSHL+GMPT+IVTNPEICRRIYLDDE FEPNYPKSVKI
Sbjct: 63 VEGDPYTFIHTLLLRYGRVGMYKSHLYGMPTIIVTNPEICRRIYLDDERFEPNYPKSVKI 122
Query: 124 LETNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEVMDK 183
LETNG+F KI+HK GYKI+ASPMNGSEVLSKHVEFIEQ VE GLEEWSSMRREPIE++D+
Sbjct: 123 LETNGDFSKIDHKSGYKIMASPMNGSEVLSKHVEFIEQTVEKGLEEWSSMRREPIELVDE 182
Query: 184 IGGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKARNKI 243
IGGL FK++LHIFLGNEID QAMAELHTLY+ELGLVIMSFLPYDLPGFTYRRALKAR KI
Sbjct: 183 IGGLLFKIILHIFLGNEIDGQAMAELHTLYKELGLVIMSFLPYDLPGFTYRRALKARKKI 242
Query: 244 EKILRCVIEKKRKRFEKDDGTTE-VYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFFAG 303
EKIL CVIEKKRKRFEKDDGT E V+ +DKLIVA NENGSK Y++ TIIDL+LG FFAG
Sbjct: 243 EKILHCVIEKKRKRFEKDDGTNEVVHCQVDKLIVATNENGSKPYNNSTIIDLILGIFFAG 302
Query: 304 HNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFINE 363
HNTPA AAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMK+LTKFINE
Sbjct: 303 HNTPAIAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKYLTKFINE 362
Query: 364 VLRRNTVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWDNY 423
VLRRNTVAPTNFRKA+T VNINGYTIPKGWTVQIWSVA+HMDSQIYSN Q+FDPSRWDNY
Sbjct: 363 VLRRNTVAPTNFRKARTYVNINGYTIPKGWTVQIWSVAIHMDSQIYSNSQEFDPSRWDNY 422
Query: 424 TPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPMG- 483
TPKPGEFIPFGLGS+FCPGSELAKLE+TILLHHF+LNYK+E VDQNC T LPS KP
Sbjct: 423 TPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMELVDQNCKVTHLPSPKPRDN 482
Query: 484 -LCRIVRVS 490
LC+I+RVS
Sbjct: 483 CLCKIIRVS 490
BLAST of CSPI03G46970 vs. NCBI nr
Match:
XP_004148515.2 (beta-amyrin 11-oxidase [Cucumis sativus] >AIT72036.1 cytochrome P450 [Cucumis sativus] >KGN60415.1 hypothetical protein Csa_001840 [Cucumis sativus])
HSP 1 Score: 665.2 bits (1715), Expect = 4.3e-187
Identity = 325/492 (66.06%), Postives = 403/492 (81.91%), Query Frame = 0
Query: 1 MELIMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNK 60
ME+I +VCVIIGVVLG + RR NE+WY KLG + YKSLPPGDLGWP +G + + K
Sbjct: 1 MEMI-SVCVIIGVVLG-VCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYK 60
Query: 61 AFRVVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKS 120
F V DP +FI + RYG+ GMYK+HL+G PTVIVTNPEICRRIYLD+ F+ +YPKS
Sbjct: 61 TFIVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKS 120
Query: 121 VKILE-TNGNFLKIEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIE 180
VKILE ++G+F ++HKI YK++ASPMNG EVLS +V+FIE+++ GLEEWSSM REPI+
Sbjct: 121 VKILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIK 180
Query: 181 VMDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGL-VIMSFLPYDLPGFTYRRALK 240
++D+IG LFFKV+ IFLG+++D + M ELHTLY+EL ++MS PYD PGFT+ + LK
Sbjct: 181 LVDEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLK 240
Query: 241 ARNKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGT 300
AR KIE ++ V+E+K +RFE +D T+EV +DKL+VA NENG+K YS++ I DL+LG
Sbjct: 241 ARRKIENTIQGVVEEKIRRFE-NDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGI 300
Query: 301 FFAGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTK 360
FFAGH+TPA AA WALLHISQNPH+FQ AKEEQESIIRQRPS QKGLT EIKQMK+LTK
Sbjct: 301 FFAGHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTK 360
Query: 361 FINEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSR 420
INEVLRRNT+ TNFR+ KT+VNING+ IPKGWTVQIW++AVHMD QIYSNPQ+F+PSR
Sbjct: 361 IINEVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSR 420
Query: 421 WDNYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAK 480
WDNYTPKPGEFIPFGLGS+FCPGSELAKLE+TILLHHF+LNYK+E V+ N+ ++PS +
Sbjct: 421 WDNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPR 480
Query: 481 PMG-LCRIVRVS 490
P LC+I+R+S
Sbjct: 481 PENCLCKIIRLS 489
BLAST of CSPI03G46970 vs. TAIR 10
Match:
AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )
HSP 1 Score: 382.5 bits (981), Expect = 5.1e-106
Identity = 209/482 (43.36%), Postives = 301/482 (62.45%), Query Frame = 0
Query: 13 VVLGFFYV--FARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNKAFRVVGDPYT 72
++LG F + +R N Y KLG K + LPPGDLGWP +G+ +F +AF+ DP +
Sbjct: 14 IILGLFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFK-TSDPES 73
Query: 73 FIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKSVKILETNGNF 132
FI +++ RYGR G+YK+H+FG P V+VT PE CRR+ DD+ F +PKS L +F
Sbjct: 74 FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 133
Query: 133 LKI---EHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEVMDKIGGL 192
+ I EHK ++ ++P+NG E LS +++FIE+ V LE+WS M IE + + L
Sbjct: 134 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE--IEFLSHLRKL 193
Query: 193 FFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKARNKIEKIL 252
FKV+++IFL +E + M L Y L + + + +LPGF Y RALKAR K+
Sbjct: 194 TFKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRA-MGINLPGFAYHRALKARKKLVAAF 253
Query: 253 RCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFFAGHNTPA 312
+ ++ +R + K + ++ +LD LI K+ENG + D+ IIDLLL AGH +
Sbjct: 254 QSIVTNRRNQ-RKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSG 313
Query: 313 TAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFINEVLRRN 372
MWA + + ++P I Q AKEEQE I+++R QK LT +E ++M +L++ I+E LR
Sbjct: 314 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 373
Query: 373 TVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWDNYTPKPG 432
T + T FR+AK++V ++GY IPKGW V W VH+D +IY +P+ FDPSRW+ YTPK G
Sbjct: 374 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 433
Query: 433 EFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPMGLC--RI 488
F+PFGLGS CPG++LAKLE++I LHHF+L Y+VE + C LP +P C RI
Sbjct: 434 TFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 486
BLAST of CSPI03G46970 vs. TAIR 10
Match:
AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )
HSP 1 Score: 382.5 bits (981), Expect = 5.1e-106
Identity = 209/482 (43.36%), Postives = 301/482 (62.45%), Query Frame = 0
Query: 13 VVLGFFYV--FARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNKAFRVVGDPYT 72
++LG F + +R N Y KLG K + LPPGDLGWP +G+ +F +AF+ DP +
Sbjct: 14 IILGLFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFK-TSDPES 73
Query: 73 FIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKSVKILETNGNF 132
FI +++ RYGR G+YK+H+FG P V+VT PE CRR+ DD+ F +PKS L +F
Sbjct: 74 FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 133
Query: 133 LKI---EHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEVMDKIGGL 192
+ I EHK ++ ++P+NG E LS +++FIE+ V LE+WS M IE + + L
Sbjct: 134 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE--IEFLSHLRKL 193
Query: 193 FFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKARNKIEKIL 252
FKV+++IFL +E + M L Y L + + + +LPGF Y RALKAR K+
Sbjct: 194 TFKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRA-MGINLPGFAYHRALKARKKLVAAF 253
Query: 253 RCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFFAGHNTPA 312
+ ++ +R + K + ++ +LD LI K+ENG + D+ IIDLLL AGH +
Sbjct: 254 QSIVTNRRNQ-RKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSG 313
Query: 313 TAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFINEVLRRN 372
MWA + + ++P I Q AKEEQE I+++R QK LT +E ++M +L++ I+E LR
Sbjct: 314 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 373
Query: 373 TVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWDNYTPKPG 432
T + T FR+AK++V ++GY IPKGW V W VH+D +IY +P+ FDPSRW+ YTPK G
Sbjct: 374 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 433
Query: 433 EFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPMGLC--RI 488
F+PFGLGS CPG++LAKLE++I LHHF+L Y+VE + C LP +P C RI
Sbjct: 434 TFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 486
BLAST of CSPI03G46970 vs. TAIR 10
Match:
AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )
HSP 1 Score: 363.6 bits (932), Expect = 2.5e-100
Identity = 193/483 (39.96%), Postives = 298/483 (61.70%), Query Frame = 0
Query: 13 VVLGFFYV--FARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNKAFRVVGDPYT 72
+VLG F + R+ N Y LG + LPPGDLGWPF+G+ L+F +AF+ DP +
Sbjct: 15 MVLGCFGLNWLVRKVNVWLYESSLGENRH-YLPPGDLGWPFIGNMLSFLRAFK-TSDPDS 74
Query: 73 FIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDDECFEPNYPKSVKILETNGNF 132
F T + RYG G+YK+H+FG P++IVT + CRR+ DD+ F+P +P S L +F
Sbjct: 75 FTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSF 134
Query: 133 LKI---EHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPIEVMDKIGGL 192
+ I EHK ++ A+P+NG E LS ++ +IE+ V L++W+ M E + + L
Sbjct: 135 VGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE--FEFLTHLRKL 194
Query: 193 FFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALKARNKIEKIL 252
F+++++IFL +E + M L Y L + + + ++PGF Y RALKAR +
Sbjct: 195 TFRIIMYIFLSSE-SENVMDALEREYTALNYGVRA-MAVNIPGFAYHRALKARKTLVAAF 254
Query: 253 RCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGTFFAGHNTPA 312
+ ++ ++R + K + + +LD L+ K+E+G K+ D+ IID+LL AGH +
Sbjct: 255 QSIVTERRNQ-RKQNILSNKKDMLDNLLNVKDEDG-KTLDDEEIIDVLLMYLNAGHESSG 314
Query: 313 TAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTKFINEVLRRN 372
MWA + + ++P + Q AK EQE I++ RP QKGL+ +E ++M+FL++ ++E LR
Sbjct: 315 HTIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVI 374
Query: 373 TVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSRWDN-YTPKP 432
T + T FR+AKT+V +NGY IPKGW V W VH+D +++ +P+ FDP+RWDN + PK
Sbjct: 375 TFSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKA 434
Query: 433 GEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPVDQNCNDTRLPSAKPMGLCRIV 490
G F+PFG GS CPG++LAKLE++I LHHF+L Y+V+ + C LP +P C +
Sbjct: 435 GAFLPFGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNC-LA 488
BLAST of CSPI03G46970 vs. TAIR 10
Match:
AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )
HSP 1 Score: 211.1 bits (536), Expect = 2.1e-54
Identity = 147/467 (31.48%), Postives = 239/467 (51.18%), Query Frame = 0
Query: 3 LIMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNKAF 62
LI V++ VVL F + W +K R LPPG +G P++G TL
Sbjct: 22 LITLTIVVVVVVLLFKW---------WLHWKEQR---LRLPPGSMGLPYIGETLRL---- 81
Query: 63 RVVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDD-ECFEPNYPKS- 122
+P +F T +YG + +K+H+ G P V++++PE R + + F+P YP S
Sbjct: 82 -YTENPNSFFATRQNKYGDI--FKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSK 141
Query: 123 VKILETNGNFLK--IEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPI 182
+++ F H +++ S S L V IE LV L W+S ++ I
Sbjct: 142 ERMIGPEALFFHQGPYHSTLKRLVQSSFMPS-ALRPTVSHIELLVLQTLSSWTS--QKSI 201
Query: 183 EVMDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALK 242
++ + F V + G++ + + + LY+ L S +P DLPG + +++K
Sbjct: 202 NTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNS-MPLDLPGTLFHKSMK 261
Query: 243 ARNKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGT 302
AR ++ + LR VIEK+R+ ++ G LL L+ AK++ SD I D ++G
Sbjct: 262 ARIELSEELRKVIEKRRENGREEGG------LLGVLLGAKDQK-RNGLSDSQIADNIIGV 321
Query: 303 FFAGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTK 362
FA +T A+ W L ++ +P++ Q EQ SI ++ + +++++ ++M T+
Sbjct: 322 IFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTR 381
Query: 363 FINEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSR 422
I E LR +V FR+A +V +GY IPKGW V +H S+ + +P+ FDPSR
Sbjct: 382 VIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSR 441
Query: 423 WDNYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPV 466
++ PKP ++PFG G CPGSELAKLEM ILLHH +++ E +
Sbjct: 442 FE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVI 457
BLAST of CSPI03G46970 vs. TAIR 10
Match:
AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )
HSP 1 Score: 211.1 bits (536), Expect = 2.1e-54
Identity = 147/467 (31.48%), Postives = 239/467 (51.18%), Query Frame = 0
Query: 3 LIMNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNKAF 62
LI V++ VVL F + W +K R LPPG +G P++G TL
Sbjct: 22 LITLTIVVVVVVLLFKW---------WLHWKEQR---LRLPPGSMGLPYIGETLRL---- 81
Query: 63 RVVGDPYTFIHTHLLRYGRVGMYKSHLFGMPTVIVTNPEICRRIYLDD-ECFEPNYPKS- 122
+P +F T +YG + +K+H+ G P V++++PE R + + F+P YP S
Sbjct: 82 -YTENPNSFFATRQNKYGDI--FKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSK 141
Query: 123 VKILETNGNFLK--IEHKIGYKIIASPMNGSEVLSKHVEFIEQLVEMGLEEWSSMRREPI 182
+++ F H +++ S S L V IE LV L W+S ++ I
Sbjct: 142 ERMIGPEALFFHQGPYHSTLKRLVQSSFMPS-ALRPTVSHIELLVLQTLSSWTS--QKSI 201
Query: 183 EVMDKIGGLFFKVVLHIFLGNEIDDQAMAELHTLYRELGLVIMSFLPYDLPGFTYRRALK 242
++ + F V + G++ + + + LY+ L S +P DLPG + +++K
Sbjct: 202 NTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNS-MPLDLPGTLFHKSMK 261
Query: 243 ARNKIEKILRCVIEKKRKRFEKDDGTTEVYSLLDKLIVAKNENGSKSYSDDTIIDLLLGT 302
AR ++ + LR VIEK+R+ ++ G LL L+ AK++ SD I D ++G
Sbjct: 262 ARIELSEELRKVIEKRRENGREEGG------LLGVLLGAKDQK-RNGLSDSQIADNIIGV 321
Query: 303 FFAGHNTPATAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKFLTK 362
FA +T A+ W L ++ +P++ Q EQ SI ++ + +++++ ++M T+
Sbjct: 322 IFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTR 381
Query: 363 FINEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWTVQIWSVAVHMDSQIYSNPQDFDPSR 422
I E LR +V FR+A +V +GY IPKGW V +H S+ + +P+ FDPSR
Sbjct: 382 VIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSR 441
Query: 423 WDNYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLHHFVLNYKVEPV 466
++ PKP ++PFG G CPGSELAKLEM ILLHH +++ E +
Sbjct: 442 FE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVI 457
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| B5BSX1 | 3.2e-113 | 43.15 | Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1 | [more] |
| Q9C5Y2 | 7.2e-105 | 43.36 | Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2 | [more] |
| Q5VRM7 | 2.7e-99 | 38.10 | Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ... | [more] |
| O23051 | 3.5e-99 | 39.96 | Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1 | [more] |
| Q9AXH9 | 4.3e-97 | 38.45 | Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0LHC2 | 3.4e-267 | 94.29 | Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa3G9038540 PE=2 SV=1 | [more] |
| A0A0A0LF52 | 2.2e-189 | 86.75 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G903530 PE=3 SV=1 | [more] |
| A0A097IYP6 | 2.1e-187 | 66.06 | Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa3G903550 PE=2 SV=1 | [more] |
| A0A6J1GLR1 | 1.7e-186 | 66.39 | beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 S... | [more] |
| A0A6J1GJM1 | 1.7e-186 | 65.84 | beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| KAE8651450.1 | 1.7e-289 | 100.00 | hypothetical protein Csa_001467 [Cucumis sativus] | [more] |
| XP_004148514.3 | 1.7e-289 | 100.00 | beta-amyrin 11-oxidase-like isoform X1 [Cucumis sativus] | [more] |
| NP_001295866.1 | 7.1e-267 | 94.29 | beta-amyrin 11-oxidase-like [Cucumis sativus] >AIT72035.1 cytochrome P450 [Cucum... | [more] |
| XP_011652660.1 | 1.6e-247 | 86.30 | beta-amyrin 11-oxidase [Cucumis sativus] | [more] |
| XP_004148515.2 | 4.3e-187 | 66.06 | beta-amyrin 11-oxidase [Cucumis sativus] >AIT72036.1 cytochrome P450 [Cucumis sa... | [more] |
