CSPI03G44420 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G44420
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Aspartate--tRNA ligase
Location	Chr3: 38060393 .. 38064032 (+)
RNA-Seq Expression	CSPI03G44420
Synteny	CSPI03G44420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTAAACCCTTTACCCAACCGAGCGGTTCGGTTCATATATGACACGAACCGAATGAACCGAGCCCCATGTTTTAGCTATTGTATTTGTTCAGCACATCTTATCGTAAGCCGCCTTCACTGACCGAGAGACGCCGTTTTCTCTCTTCCATTTTCCTTCAAAACCCTCAATCTCTCTTCTCATTTCCTTATACTCCAACCATGTCCGCTTCATCCCAAACTCCGATCGACGAGCCGCCTTCCGCCGATGATGATTCTTCCAAAGCCGTCAGCAAGAAAGCCGCCAAGAAGGAGGCTGCCAAGCAAGAAAAGCTCCGACGTCGCCAAGAAGCAGCTGCAGAATCTGCTATTTCGTCTCTCAACGTCGAGGACGATCCGCTTTCCGCTAACTACGGCGATGTACCACTCTCCGATCTCCAATCAAAGGAGGTTAAGAGTATTGAAAACTGGACTCAGGTTGGCTCTTTGACGCCGGAGCTTAAAGACAAGTATGTGATTCTACGGGGTCGTGTTCAGACGATTCGTGCTGTTGGGAAGAAGATGGCGTTTTTGGTGGTGAGAGAAAAGGGATTCACCGTGCAATGCGTTTTGTCGGAGCAGCCGGAGCTTGTTAGTCGCCAGATGGTGAAGTATGTTGATGGATTGAGCCGTGAGTCCATCGTTGACGTTGAAGGAGTTGTGTCTGTGCCCAATGTTGCCATTAAAGGTGCTTCGCAACAGGTGGAAATTCAAGTGAGGAAAGTATATTGCATCAGTAAGGCAATGCCGACTTTGCCTATAAATATCGAAGATGCTGCCCGTAGTGAAGCTGAAATTGATAAGGCCTTGCAGGTATAATTTGTAATCGCTTTCTTTTAATGTGATCTTCTTCGTGATTATATATATATATATATATGTTCTTGGTTTCACTCTGTAGTGCTTTTGAACCAAAAGCAGAGAGATTTGTGAACCTTCTGTTTAATGTTAAACTCATTTCTTGTCCGCAGGCTGGTGAGCAACTGGTACGTGTTAATCAGGATACGCGTTTGAACTACAGAGTCCTTGATATGCGAACTCCAGCTAATCAAGGGATTTTTCGCATCCAATGTCAAGTTTCAACGGTGAGTTCTCTTTGTTATTTTATATTTTTAAAGGTTCTTTGATCTAAATCGATGGTGATATTACATGAAATTTAAGTATGCGGGCTTTATTCAAAGTTGATCATGGTAGCTATAGCTATCATTGTCGGCCTGCTTGGTTCGGTTCTTGTTGCCTTGTTCATTAGCCATCTGTAGCTTGTAGGAAGAGTCGATTTGTTTTGTGGGGTTGGACTTCTGGGGAGGATCTTTGGGCTTTGATTATATTAAAATCCTCTTTCTGGATGTATCTTAATAGTTTTGGTAATTATCAGCTTGGTCTTATTATTCTTTTGGGTGTGAGGGTTGTTTTGCCTCTGTGATTGTTTTTTATATTGTATGCCCTTGTTTATTCTTTCAATATTTTCTATGAAAACAAGATTTCTTATCTTTAAAAATCTTATGGCTGCCATTAATCACTCCTTTTGCTCTCTTTGGTGTGAAAAGGAATTGGAATTCTTTCATGTAACTCACGGTTGTTTACAATAAGATTGTAAACAAGGAATTTGTAATGTTAAGCTATTTCCGCATGTAGTTCATGTAGTTGCACTTCTCATGTTAAATGAAGCACAGTCGTTGATTATATTTTCATCATGCAGATGTTTAGACAATTTCTGTTGGATGAGAACTTTGTTGAAATCCACACTCCAAAATTGATTGGAGGTTCTAGTGAAGGTGGTGCTTCTGTTTTTAGGTTGGATTACAAAGGCCAACCTGCTTGCCTGGCTCAGTCACCTCAGTTACATAAGCAAATGGCAATCTGTGGTGACTTTGGGCGTGTATTTGAGATTGGTCCTGTTTTTAGAGCAGAAGACTCATACACACACAGACATTTATGTGAATTTACAGGACTTGATGTTGAAATGGAGATCAAGACTCACTATTCTGAGGTAATGCTCTAAGTTGGCTTTTCATTTGTCTTGGGCATAGTTATCTAATATTTGATCTGACTTTTTTTCTTCTGTTTTAGTGCAGGTCATGGACATTGTGGACCGTTTATTTGTTGCAATGTTTGATAGTTTGAATGAAAAATGTAAGAAAGCGCTTGAAGCAGTTGATAAACAGTATCCCTTTGAACCTTTAAAGGTGAACTTATTTATTGATTATGCTTACTTTGTAAATGTTTCATACGCTTCGAAGGTGAACTTATTTTTGCTTTGGATTTATGCCAATAGAAGACATGTACAAGTACAATGTGGATATATGACTGAGTTTGGATAGTTGAATAATTTTCTCATTTCTATCTTTGATTCAATTTCATGAATATGCAGTATTTGCGAAAAACTCTACGACTTACATTTGAGGAAGGAATTCAAATGTTGAAGGTTAGTACATAGTGATACCGACCCTTTTTCCCTTACTAATCATGATAAGATTCCAAGTCCCAAATGTCTTGACATTTTTTTCTTTCTTCGTTATGTATGATTATCATCATAGGATGCTGGCGTCGAAATTGATCCTCTTGGGGATCTTAATACAGAGGCTGAGAGAAAATTGGGACAACTCGTCTTAGAGAAGTAATTAGGGGTTCTTGCTTATTTAGTTATGAACTTGTTGATTTTGTTCAACTATCATATCTAAAGATTTCTGATCAAACTTTCCTAACTATAGATATGGCACGGAGTTCTACATTCTTCACCGCTATCCTTTGGCTGTACGGCCATTCTACACTATGCCTTGCTACGATAACCCAACTTACAGTAACTCATTTGACGTCTTTATTCGAGGTATACATTGCATATTATTACATCACTAGAATTTTCTTACCAGTGCTTATTCTTCCTTAAAATTCGGGTTCATATTCATTCTACGGAACAAAATTTGACAATACATCCTCCATGGTAAGTAACTTGAAACATTGTTCTCCACGCAGGGGAAGAAATCATTTCTGGGGCTCAGCGTGTGCACGTGCCAGAGTTTCTAGCTGAACGCGCTCAAGCATGTGGCATTGACGTAAAAACAATAGAAACATATATCGATTCATTCAGGTACATTTCTCTTTGTTTTCTCCTTTACTCAAAGTTGGAATTTAACCAAATAAGCAAGATTCAATTAGGTCCGACTCCGACATTTTTTGTGACACGGATGCTTTCATTGCAGATATGGTGCCCCTCCGCACGGTGGATTTGGAGTAGGTTTGGAGCGGGTGGTGATGCTGTTTTGTGGTTTGAATAACATTCGCAAAACATCATTGTTCCCTCGTGATCCACAGAGGATTGCTCCATAGGAATTTCACAAGTAACGCTGCAATTCCAGTTTTTGAAATATACATTTTGTTCCCTTCTTTCTATCTTTCTTTCTCCCTTCATAGGAAAGGAAAACAAACAAATTTCTGATATACTGATTATCGTGGTTTACTGAATAACAATTTGTTTTCATCGTTGCGAATTTTCCAAGGTTGTTTTGACAACCGTTACTTCATGTAGATTTTTGTTATAGTTTTCTTTGTTTGAAAATGATAATTTGACAAATTCTCCAAGTCAAAACCTTTGTTTTTTCTTTTTTTGGTTAAAGAATTGAAGAACTCT

mRNA sequence

CCTAAACCCTTTACCCAACCGAGCGGTTCGGTTCATATATGACACGAACCGAATGAACCGAGCCCCATGTTTTAGCTATTGTATTTGTTCAGCACATCTTATCGTAAGCCGCCTTCACTGACCGAGAGACGCCGTTTTCTCTCTTCCATTTTCCTTCAAAACCCTCAATCTCTCTTCTCATTTCCTTATACTCCAACCATGTCCGCTTCATCCCAAACTCCGATCGACGAGCCGCCTTCCGCCGATGATGATTCTTCCAAAGCCGTCAGCAAGAAAGCCGCCAAGAAGGAGGCTGCCAAGCAAGAAAAGCTCCGACGTCGCCAAGAAGCAGCTGCAGAATCTGCTATTTCGTCTCTCAACGTCGAGGACGATCCGCTTTCCGCTAACTACGGCGATGTACCACTCTCCGATCTCCAATCAAAGGAGGTTAAGAGTATTGAAAACTGGACTCAGGTTGGCTCTTTGACGCCGGAGCTTAAAGACAAGTATGTGATTCTACGGGGTCGTGTTCAGACGATTCGTGCTGTTGGGAAGAAGATGGCGTTTTTGGTGGTGAGAGAAAAGGGATTCACCGTGCAATGCGTTTTGTCGGAGCAGCCGGAGCTTGTTAGTCGCCAGATGGTGAAGTATGTTGATGGATTGAGCCGTGAGTCCATCGTTGACGTTGAAGGAGTTGTGTCTGTGCCCAATGTTGCCATTAAAGGTGCTTCGCAACAGGTGGAAATTCAAGTGAGGAAAGTATATTGCATCAGTAAGGCAATGCCGACTTTGCCTATAAATATCGAAGATGCTGCCCGTAGTGAAGCTGAAATTGATAAGGCCTTGCAGGCTGGTGAGCAACTGGTACGTGTTAATCAGGATACGCGTTTGAACTACAGAGTCCTTGATATGCGAACTCCAGCTAATCAAGGGATTTTTCGCATCCAATGTCAAGTTTCAACGATGTTTAGACAATTTCTGTTGGATGAGAACTTTGTTGAAATCCACACTCCAAAATTGATTGGAGGTTCTAGTGAAGGTGGTGCTTCTGTTTTTAGGTTGGATTACAAAGGCCAACCTGCTTGCCTGGCTCAGTCACCTCAGTTACATAAGCAAATGGCAATCTGTGGTGACTTTGGGCGTGTATTTGAGATTGGTCCTGTTTTTAGAGCAGAAGACTCATACACACACAGACATTTATGTGAATTTACAGGACTTGATGTTGAAATGGAGATCAAGACTCACTATTCTGAGGTCATGGACATTGTGGACCGTTTATTTGTTGCAATGTTTGATAGTTTGAATGAAAAATGTAAGAAAGCGCTTGAAGCAGTTGATAAACAGTATCCCTTTGAACCTTTAAAGTATTTGCGAAAAACTCTACGACTTACATTTGAGGAAGGAATTCAAATGTTGAAGGATGCTGGCGTCGAAATTGATCCTCTTGGGGATCTTAATACAGAGGCTGAGAGAAAATTGGGACAACTCGTCTTAGAGAAATATGGCACGGAGTTCTACATTCTTCACCGCTATCCTTTGGCTGTACGGCCATTCTACACTATGCCTTGCTACGATAACCCAACTTACAGTAACTCATTTGACGTCTTTATTCGAGGGGAAGAAATCATTTCTGGGGCTCAGCGTGTGCACGTGCCAGAGTTTCTAGCTGAACGCGCTCAAGCATGTGGCATTGACGTAAAAACAATAGAAACATATATCGATTCATTCAGATATGGTGCCCCTCCGCACGGTGGATTTGGAGTAGGTTTGGAGCGGGTGGTGATGCTGTTTTGTGGTTTGAATAACATTCGCAAAACATCATTGTTCCCTCGTGATCCACAGAGGATTGCTCCATAGGAATTTCACAAGTAACGCTGCAATTCCAGTTTTTGAAATATACATTTTGTTCCCTTCTTTCTATCTTTCTTTCTCCCTTCATAGGAAAGGAAAACAAACAAATTTCTGATATACTGATTATCGTGGTTTACTGAATAACAATTTGTTTTCATCGTTGCGAATTTTCCAAGGTTGTTTTGACAACCGTTACTTCATGTAGATTTTTGTTATAGTTTTCTTTGTTTGAAAATGATAATTTGACAAATTCTCCAAGTCAAAACCTTTGTTTTTTCTTTTTTTGGTTAAAGAATTGAAGAACTCT

Coding sequence (CDS)

ATGTCCGCTTCATCCCAAACTCCGATCGACGAGCCGCCTTCCGCCGATGATGATTCTTCCAAAGCCGTCAGCAAGAAAGCCGCCAAGAAGGAGGCTGCCAAGCAAGAAAAGCTCCGACGTCGCCAAGAAGCAGCTGCAGAATCTGCTATTTCGTCTCTCAACGTCGAGGACGATCCGCTTTCCGCTAACTACGGCGATGTACCACTCTCCGATCTCCAATCAAAGGAGGTTAAGAGTATTGAAAACTGGACTCAGGTTGGCTCTTTGACGCCGGAGCTTAAAGACAAGTATGTGATTCTACGGGGTCGTGTTCAGACGATTCGTGCTGTTGGGAAGAAGATGGCGTTTTTGGTGGTGAGAGAAAAGGGATTCACCGTGCAATGCGTTTTGTCGGAGCAGCCGGAGCTTGTTAGTCGCCAGATGGTGAAGTATGTTGATGGATTGAGCCGTGAGTCCATCGTTGACGTTGAAGGAGTTGTGTCTGTGCCCAATGTTGCCATTAAAGGTGCTTCGCAACAGGTGGAAATTCAAGTGAGGAAAGTATATTGCATCAGTAAGGCAATGCCGACTTTGCCTATAAATATCGAAGATGCTGCCCGTAGTGAAGCTGAAATTGATAAGGCCTTGCAGGCTGGTGAGCAACTGGTACGTGTTAATCAGGATACGCGTTTGAACTACAGAGTCCTTGATATGCGAACTCCAGCTAATCAAGGGATTTTTCGCATCCAATGTCAAGTTTCAACGATGTTTAGACAATTTCTGTTGGATGAGAACTTTGTTGAAATCCACACTCCAAAATTGATTGGAGGTTCTAGTGAAGGTGGTGCTTCTGTTTTTAGGTTGGATTACAAAGGCCAACCTGCTTGCCTGGCTCAGTCACCTCAGTTACATAAGCAAATGGCAATCTGTGGTGACTTTGGGCGTGTATTTGAGATTGGTCCTGTTTTTAGAGCAGAAGACTCATACACACACAGACATTTATGTGAATTTACAGGACTTGATGTTGAAATGGAGATCAAGACTCACTATTCTGAGGTCATGGACATTGTGGACCGTTTATTTGTTGCAATGTTTGATAGTTTGAATGAAAAATGTAAGAAAGCGCTTGAAGCAGTTGATAAACAGTATCCCTTTGAACCTTTAAAGTATTTGCGAAAAACTCTACGACTTACATTTGAGGAAGGAATTCAAATGTTGAAGGATGCTGGCGTCGAAATTGATCCTCTTGGGGATCTTAATACAGAGGCTGAGAGAAAATTGGGACAACTCGTCTTAGAGAAATATGGCACGGAGTTCTACATTCTTCACCGCTATCCTTTGGCTGTACGGCCATTCTACACTATGCCTTGCTACGATAACCCAACTTACAGTAACTCATTTGACGTCTTTATTCGAGGGGAAGAAATCATTTCTGGGGCTCAGCGTGTGCACGTGCCAGAGTTTCTAGCTGAACGCGCTCAAGCATGTGGCATTGACGTAAAAACAATAGAAACATATATCGATTCATTCAGATATGGTGCCCCTCCGCACGGTGGATTTGGAGTAGGTTTGGAGCGGGTGGTGATGCTGTTTTGTGGTTTGAATAACATTCGCAAAACATCATTGTTCCCTCGTGATCCACAGAGGATTGCTCCATAG

Protein sequence

MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPLSANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVREKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRKVYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIFRIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDSLNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP*

Homology

[Close]

BLAST of CSPI03G44420 vs. ExPASy Swiss-Prot
Match: Q9M084 (Aspartate--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=IBI1 PE=1 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 6.8e-213
Identity = 381/557 (68.40%), Postives = 445/557 (79.89%), Query Frame = 0


Query: 2   SASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQ---EAAAESAISSLNVEDD 61
           S S   P+    +A ++S +  SKKAAKKEAAK EKLRRRQ   EA   +A  SL  E+D
Sbjct: 3   SESEIPPLSSSTAAAEESGEKTSKKAAKKEAAKLEKLRRRQEQEEATRRTASISLE-END 62

Query: 62  PLSANYGDVPLSDLQS-------KEVKSIE--NWTQVGSLTPELKDKYVILRGRVQTIRA 121
             S NYGDV L++LQS       K ++++E   WT V  L  E+ +  V++RGRV T R 
Sbjct: 63  EFSNNYGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRGRVHTNRP 122

Query: 122 VGKKMAFLVVREKGFTVQCVLSEQPEL-VSRQMVKYVDGLSRESIVDVEGVVSVPNVAIK 181
              K+ F+V+RE G TVQCV+S+  +  V   MVKY+  LSRES VDV GVV++P   + 
Sbjct: 123 TSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDVIGVVTLPKEPLT 182

Query: 182 GASQQVEIQVRKVYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRV 241
           G +QQVEIQVRKVYCI+K++  LP+++EDAARSEA+I+ +LQ      RVNQDTRLNYRV
Sbjct: 183 GTTQQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNYRV 242

Query: 242 LDMRTPANQGIFRIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPA 301
           LD+RTPANQ IF++Q +V   FR+ L  +NFV IHTPKL+ GSSEGG++VFRL+YKGQPA
Sbjct: 243 LDLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEYKGQPA 302

Query: 302 CLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMD 361
           CLAQSPQLHKQMAICGD  RVFE+GPVFRAEDS+THRHLCEF GLDVEMEI+ HYSE+MD
Sbjct: 303 CLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRKHYSEIMD 362

Query: 362 IVDRLFVAMFDSLNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDP 421
           +VD LFV +F SLNE+CKK L+AV KQYPFEPLK+L KTLRLTFEEG+QMLK+AGVE+DP
Sbjct: 363 LVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEVDP 422

Query: 422 LGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEE 481
           LGDLNTE+ERKLGQLVLEKY TEFYILHRYP AVRPFYTM C DNP YSNSFDVFIRGEE
Sbjct: 423 LGDLNTESERKLGQLVLEKYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRGEE 482

Query: 482 IISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLN 541
           IISGAQRVH+PE L +RA  CGIDVKTI TYIDSFRYGAP HGGFGVGLERVVMLFC LN
Sbjct: 483 IISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALN 542

Query: 542 NIRKTSLFPRDPQRIAP 546
           NIRKTSLFPRDPQR++P
Sbjct: 543 NIRKTSLFPRDPQRLSP 558

[Close]

BLAST of CSPI03G44420 vs. ExPASy Swiss-Prot
Match: Q8H104 (Aspartate--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g26870 PE=2 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 6.9e-197
Identity = 350/531 (65.91%), Postives = 422/531 (79.47%), Query Frame = 0


Query: 17  DDSSKAVSKKAAKKEAAKQEKLRRRQE-AAAESAISSLNVEDDPLSANYGDVPLSDLQSK 76
           ++  + +SKK +KK AAK EKL R+QE   A S+  SL  ED+  S+NYGDV  ++LQS 
Sbjct: 8   EECGEKISKKESKKRAAKLEKLLRKQEREEATSSSLSLEEEDESCSSNYGDVTTNELQSA 67

Query: 77  -EVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVREKGFTVQCVLSEQP 136
            E K +   T V +L  E+    V +RGR+   R VG K+ F+++RE GFTVQCV+ E  
Sbjct: 68  VEGKEL---TDVSNLVEEIVGSEVSIRGRLHKNRLVGTKL-FVILRESGFTVQCVVEE-- 127

Query: 137 ELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRKVYCISKAMPTLPIN 196
             V   M+K+V  LSRES+V++ GVVS P   + G +QQVEI VRK+YC+S+++P LP+ 
Sbjct: 128 TRVGANMIKFVKQLSRESVVELIGVVSHPKKPLTGTTQQVEIHVRKMYCLSRSLPNLPLV 187

Query: 197 IEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIFRIQCQVSTMFRQFL 256
           +EDAARSE++I+K+ + G+Q  RV QDTRLN RVLD+RTPANQ IFRIQCQV   FR++L
Sbjct: 188 VEDAARSESDIEKSGKDGKQAARVLQDTRLNNRVLDIRTPANQAIFRIQCQVQIAFREYL 247

Query: 257 LDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGP 316
             + F+EIHTPKLI GSSEGG++VFRLDYKGQPACLAQSPQLHKQMAICGD  RVFE+GP
Sbjct: 248 QSKGFLEIHTPKLIAGSSEGGSAVFRLDYKGQPACLAQSPQLHKQMAICGDMRRVFEVGP 307

Query: 317 VFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDSLNEKCKKALEAVDK 376
           VFRAEDS+THRHLCEF GLDVEMEI+ HYSE+MD+V  LF  +F  + E+C K LE+V K
Sbjct: 308 VFRAEDSFTHRHLCEFVGLDVEMEIRMHYSEIMDLVGELFPFIFTKIEERCPKELESVRK 367

Query: 377 QYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKLGQLVLEKYGTEFYI 436
           QYPF+ LK+L +TLRLTF EGIQMLK+AG E+DPLGDLNTE+ERKLGQLVLEKY TEFY+
Sbjct: 368 QYPFQSLKFLPQTLRLTFAEGIQMLKEAGEEVDPLGDLNTESERKLGQLVLEKYKTEFYM 427

Query: 437 LHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVK 496
           LHRYP AVRPFYTMP  ++  YSNSFDVFIRGEEI+SGAQR+H PE L +RA+ CGIDVK
Sbjct: 428 LHRYPSAVRPFYTMPYENDSNYSNSFDVFIRGEEIMSGAQRIHDPELLEKRARECGIDVK 487

Query: 497 TIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 546
           TI TYID+FRYGAPPHGGFGVGLERVVML C LNNIRKTSLFPRD QR+ P
Sbjct: 488 TISTYIDAFRYGAPPHGGFGVGLERVVMLLCALNNIRKTSLFPRDSQRLTP 532

[Close]

BLAST of CSPI03G44420 vs. ExPASy Swiss-Prot
Match: Q5R9I5 (Aspartate--tRNA ligase, cytoplasmic OS=Pongo abelii OX=9601 GN=DARS1 PE=2 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 2.8e-134
Identity = 260/520 (50.00%), Postives = 349/520 (67.12%), Query Frame = 0


Query: 27  AAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPLSANYGDVPLSDLQSKEVKSIENWTQV 86
           +A      QEK R   +AA           +D     YG   +S +   + KS     +V
Sbjct: 3   SASASRKSQEKPREIMDAA-----------EDYAKERYG---ISSMIQSQEKSDRVLVRV 62

Query: 87  GSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVREKGFTVQCVLSEQPELVSRQMVKYVD 146
             LT +  D+ V +R RV T RA GK+  FLV+R++ F VQ +++   +  S+QMVK+  
Sbjct: 63  RDLTIQKADEVVWVRARVHTSRAKGKQ-CFLVLRQQQFNVQALVA-VGDHASKQMVKFAA 122

Query: 147 GLSRESIVDVEGVVSVPNVAIKGASQQ-VEIQVRKVYCISKAMPTLPINIEDAARSEAEI 206
            +++ESIVDVEGVV   N  I+  +QQ VE+ V+K+Y IS A P LP+ ++DA R EAE 
Sbjct: 123 NINKESIVDVEGVVRKVNQKIESCTQQDVELHVQKIYVISLAEPRLPLQLDDAVRPEAEG 182

Query: 207 DKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIFRIQCQVSTMFRQFLLDENFVEIHTP 266
           ++     E    VNQDTRL+ RV+D+RT  +Q +FR+Q  +  +FR+ L+++ FVEI TP
Sbjct: 183 EE-----EGRATVNQDTRLDNRVIDLRTSTSQAVFRLQSGICHLFRETLINKGFVEIQTP 242

Query: 267 KLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSYTHR 326
           K+I  +SEGGA+VF + Y    A LAQSPQL+KQM IC DF +VF IGPVFRAEDS THR
Sbjct: 243 KIISAASEGGANVFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFCIGPVFRAEDSNTHR 302

Query: 327 HLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDSLNEKCKKALEAVDKQYPFEPLKYLR 386
           HL EF GLD+EM    HY EVM+ +    V +F  L E+ +  ++ V+KQ+P EP K+L 
Sbjct: 303 HLTEFVGLDIEMAFNYHYHEVMEEIADTMVQIFKGLQERFQTEIQTVNKQFPCEPFKFLE 362

Query: 387 KTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPF 446
            TLRL + E + ML++AGVE+    DL+T  E+ LG L+ EKY T+FYIL +YPLAVRPF
Sbjct: 363 PTLRLEYCEALAMLREAGVEMGDEDDLSTPNEKLLGHLIKEKYDTDFYILDKYPLAVRPF 422

Query: 447 YTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRY 506
           YTMP   NP  SNS+D+F+RGEEI+SGAQR+H P+ L ERA   GID++ I+ YIDSFR+
Sbjct: 423 YTMPDPRNPKQSNSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRF 482

Query: 507 GAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 546
           GAPPH G G+GLERV MLF GL+N+R+TS+FPRDP+R+ P
Sbjct: 483 GAPPHAGGGIGLERVTMLFLGLHNVRQTSMFPRDPKRLTP 501

[Close]

BLAST of CSPI03G44420 vs. ExPASy Swiss-Prot
Match: Q922B2 (Aspartate--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Dars1 PE=1 SV=2)

HSP 1 Score: 479.6 bits (1233), Expect = 4.8e-134
Identity = 251/494 (50.81%), Postives = 340/494 (68.83%), Query Frame = 0


Query: 53  LNVEDDPLSANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGK 112
           ++  +D     YG   +S +   + K      +V  LT +  D  V +R RV T RA GK
Sbjct: 18  VDAAEDYAKERYG---ISSMIQSQEKPDRVLVRVKDLTVQKADDVVWVRARVHTSRAKGK 77

Query: 113 KMAFLVVREKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQ 172
           +  FLV+R++ F VQ +++   +  S+QMVK+   +++ESI+DVEGVV   N  I   +Q
Sbjct: 78  Q-CFLVLRQQQFNVQALVA-VGDHASKQMVKFAANINKESIIDVEGVVRKVNQKIGSCTQ 137

Query: 173 Q-VEIQVRKVYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDM 232
           Q VE+ V+K+Y IS A P LP+ ++DA R E E ++  +A      VNQDTRL+ RV+D+
Sbjct: 138 QDVELHVQKIYVISLAEPRLPLQLDDAIRPEVEGEEDGRA-----TVNQDTRLDNRVIDL 197

Query: 233 RTPANQGIFRIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLA 292
           RT  +Q IFR+Q  +  +FR+ L+++ FVEI TPK+I  +SEGGA+VF + Y    A LA
Sbjct: 198 RTSTSQAIFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGANVFTVSYFKNNAYLA 257

Query: 293 QSPQLHKQMAICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVD 352
           QSPQL+KQM IC DF +VF IGPVFRAEDS THRHL EF GLD+EM    HY EV++ + 
Sbjct: 258 QSPQLYKQMCICADFEKVFCIGPVFRAEDSNTHRHLTEFVGLDIEMAFNYHYHEVVEEIA 317

Query: 353 RLFVAMFDSLNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGD 412
              V +F  L E+ +  ++ V KQ+P EP K+L  TLRL + E + ML++AGVE+D   D
Sbjct: 318 DTLVQIFKGLQERFQTEIQTVSKQFPCEPFKFLEPTLRLEYCEALAMLREAGVEMDDEED 377

Query: 413 LNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIIS 472
           L+T  E+ LG+LV EKY T+FY+L +YPLAVRPFYTMP   NP  SNS+D+F+RGEEI+S
Sbjct: 378 LSTPNEKLLGRLVKEKYDTDFYVLDKYPLAVRPFYTMPDPRNPKQSNSYDMFMRGEEILS 437

Query: 473 GAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIR 532
           GAQR+H P+ L ERA   GID++ I+ YIDSFR+GAPPH G G+GLERV MLF GL+N+R
Sbjct: 438 GAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGLERVTMLFLGLHNVR 497

Query: 533 KTSLFPRDPQRIAP 546
           +TS+FPRDP+R+ P
Sbjct: 498 QTSMFPRDPKRLTP 501

[Close]

BLAST of CSPI03G44420 vs. ExPASy Swiss-Prot
Match: P14868 (Aspartate--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=DARS1 PE=1 SV=2)

HSP 1 Score: 479.2 bits (1232), Expect = 6.3e-134
Identity = 260/520 (50.00%), Postives = 347/520 (66.73%), Query Frame = 0


Query: 27  AAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPLSANYGDVPLSDLQSKEVKSIENWTQV 86
           +A      QEK R   +AA           +D     YG   +S +   + K      +V
Sbjct: 3   SASASRKSQEKPREIMDAA-----------EDYAKERYG---ISSMIQSQEKPDRVLVRV 62

Query: 87  GSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVREKGFTVQCVLSEQPELVSRQMVKYVD 146
             LT +  D+ V +R RV T RA GK+  FLV+R++ F VQ +++   +  S+QMVK+  
Sbjct: 63  RDLTIQKADEVVWVRARVHTSRAKGKQ-CFLVLRQQQFNVQALVA-VGDHASKQMVKFAA 122

Query: 147 GLSRESIVDVEGVVSVPNVAIKGASQQ-VEIQVRKVYCISKAMPTLPINIEDAARSEAEI 206
            +++ESIVDVEGVV   N  I   +QQ VE+ V+K+Y IS A P LP+ ++DA R EAE 
Sbjct: 123 NINKESIVDVEGVVRKVNQKIGSCTQQDVELHVQKIYVISLAEPRLPLQLDDAVRPEAEG 182

Query: 207 DKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIFRIQCQVSTMFRQFLLDENFVEIHTP 266
           ++     E    VNQDTRL+ RV+D+RT  +Q +FR+Q  +  +FR+ L+++ FVEI TP
Sbjct: 183 EE-----EGRATVNQDTRLDNRVIDLRTSTSQAVFRLQSGICHLFRETLINKGFVEIQTP 242

Query: 267 KLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSYTHR 326
           K+I  +SEGGA+VF + Y    A LAQSPQL+KQM IC DF +VF IGPVFRAEDS THR
Sbjct: 243 KIISAASEGGANVFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFSIGPVFRAEDSNTHR 302

Query: 327 HLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDSLNEKCKKALEAVDKQYPFEPLKYLR 386
           HL EF GLD+EM    HY EVM+ +    V +F  L E+ +  ++ V+KQ+P EP K+L 
Sbjct: 303 HLTEFVGLDIEMAFNYHYHEVMEEIADTMVQIFKGLQERFQTEIQTVNKQFPCEPFKFLE 362

Query: 387 KTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPF 446
            TLRL + E + ML++AGVE+    DL+T  E+ LG LV EKY T+FYIL +YPLAVRPF
Sbjct: 363 PTLRLEYCEALAMLREAGVEMGDEDDLSTPNEKLLGHLVKEKYDTDFYILDKYPLAVRPF 422

Query: 447 YTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRY 506
           YTMP   NP  SNS+D+F+RGEEI+SGAQR+H P+ L ERA   GID++ I+ YIDSFR+
Sbjct: 423 YTMPDPRNPKQSNSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRF 482

Query: 507 GAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 546
           GAPPH G G+GLERV MLF GL+N+R+TS+FPRDP+R+ P
Sbjct: 483 GAPPHAGGGIGLERVTMLFLGLHNVRQTSMFPRDPKRLTP 501

[Close]

BLAST of CSPI03G44420 vs. ExPASy TrEMBL
Match: A0A0A0LDS7 (Aspartyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_3G881580 PE=3 SV=1)

HSP 1 Score: 1074.7 bits (2778), Expect = 2.0e-310
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0


Query: 1   MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL
Sbjct: 1   MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK
Sbjct: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360

Query: 361 LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

[Close]

BLAST of CSPI03G44420 vs. ExPASy TrEMBL
Match: A0A5A7TBL8 (Aspartyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006980 PE=3 SV=1)

HSP 1 Score: 1058.9 bits (2737), Expect = 7.1e-306
Identity = 537/545 (98.53%), Postives = 542/545 (99.45%), Query Frame = 0


Query: 1   MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASSQTPIDEP SA D+SSKAVSKKAAKKEAAK EKLRRRQEAAAESAISSLNVEDDPL
Sbjct: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGR+QTIRAVGKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK
Sbjct: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMPTLPINIEDAARSEAEI+KALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQVSTMFRQFLLDE+FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360

Query: 361 LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           LNE+CKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

[Close]

BLAST of CSPI03G44420 vs. ExPASy TrEMBL
Match: A0A1S3CR09 (Aspartyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503781 PE=3 SV=1)

HSP 1 Score: 1058.9 bits (2737), Expect = 7.1e-306
Identity = 537/545 (98.53%), Postives = 542/545 (99.45%), Query Frame = 0


Query: 1   MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASSQTPIDEP SA D+SSKAVSKKAAKKEAAK EKLRRRQEAAAESAISSLNVEDDPL
Sbjct: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGR+QTIRAVGKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK
Sbjct: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMPTLPINIEDAARSEAEI+KALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQVSTMFRQFLLDE+FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360

Query: 361 LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           LNE+CKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

[Close]

BLAST of CSPI03G44420 vs. ExPASy TrEMBL
Match: A0A6J1FFA5 (Aspartate--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111443419 PE=3 SV=1)

HSP 1 Score: 1013.8 bits (2620), Expect = 2.6e-292
Identity = 509/545 (93.39%), Postives = 529/545 (97.06%), Query Frame = 0


Query: 1   MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASS+TPIDEP SA D++SKAVSKKAAKKEAAK EKLRRRQEAAAE+AISSLNVEDDPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKE K + NWTQVGSLT ELKDKYVILRGR+QTIRAV KKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCV+SEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGA+QQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMP+LPINIEDAARSEAEI+KALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQV T+FRQFLL E FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           +ICGDFGRVFEIGPVFRAEDS+THRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFV+MFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 361 LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           +N+ CKK LEA+DKQYPFEPLKYLR TLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FL ERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLTERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

[Close]

BLAST of CSPI03G44420 vs. ExPASy TrEMBL
Match: A0A6J1ILA3 (Aspartate--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111476621 PE=3 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 3.8e-291
Identity = 508/545 (93.21%), Postives = 528/545 (96.88%), Query Frame = 0


Query: 1   MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASS+TPIDE  SA D++SKAVSKKAAKKEAAK EKLRRRQEAAAE+AISSLNVEDDPL
Sbjct: 1   MSASSETPIDETLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKE K + NWTQVGSLT ELKDKYVILRGR+QTIRAV KKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCV+SEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGA+QQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMP+LPINIEDAARSEAEI+KALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQV T+FRQFLL E FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           +ICGDFGRVFEIGPVFRAEDS+THRHLCEFTGLDVEMEIKTHY EVMDIVDRLFV+MFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYYEVMDIVDRLFVSMFDS 360

Query: 361 LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           +N+ CKK LEA+DKQYPFEPLKYLR TLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

[Close]

BLAST of CSPI03G44420 vs. NCBI nr
Match: XP_004136326.1 (aspartate--tRNA ligase 2, cytoplasmic [Cucumis sativus] >KGN60145.1 hypothetical protein Csa_002506 [Cucumis sativus])

HSP 1 Score: 1074.7 bits (2778), Expect = 4.2e-310
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0


Query: 1   MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL
Sbjct: 1   MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK
Sbjct: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360

Query: 361 LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

[Close]

BLAST of CSPI03G44420 vs. NCBI nr
Match: XP_008466345.1 (PREDICTED: aspartate--tRNA ligase 2, cytoplasmic [Cucumis melo] >KAA0038775.1 aspartate--tRNA ligase 2 [Cucumis melo var. makuwa] >TYK31390.1 aspartate--tRNA ligase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1058.9 bits (2737), Expect = 1.5e-305
Identity = 537/545 (98.53%), Postives = 542/545 (99.45%), Query Frame = 0


Query: 1   MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASSQTPIDEP SA D+SSKAVSKKAAKKEAAK EKLRRRQEAAAESAISSLNVEDDPL
Sbjct: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGR+QTIRAVGKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK
Sbjct: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMPTLPINIEDAARSEAEI+KALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQVSTMFRQFLLDE+FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360

Query: 361 LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           LNE+CKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

[Close]

BLAST of CSPI03G44420 vs. NCBI nr
Match: XP_023535979.1 (aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1015.0 bits (2623), Expect = 2.4e-292
Identity = 510/545 (93.58%), Postives = 529/545 (97.06%), Query Frame = 0


Query: 1   MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASS+TPIDEP SA D++SKAVSKKAAKKEAAK EKLRRRQEA AE+AISSLNVEDDPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKE K + NWTQVGSLT ELKDKYVILRGR+QTIRAV KKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCV+SEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGA+QQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMP+LPINIEDAARSEAEI+KALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQV T+FRQFLL E FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           AICGDFGRVFEIGPVFRAEDS+THRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFV+MFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 361 LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           +N+ CKK LEA+DKQYPFEPLKYLR TLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

[Close]

BLAST of CSPI03G44420 vs. NCBI nr
Match: KAG6591612.1 (Aspartate--tRNA ligase 2, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1013.8 bits (2620), Expect = 5.4e-292
Identity = 509/545 (93.39%), Postives = 530/545 (97.25%), Query Frame = 0


Query: 1   MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASS+TPIDEP SA D++SKAVSKKAAKKEAAK EKLRRRQEAAAE+AISSLNVEDDPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKE K + NWTQVGSLT ELK+KYVILRGR+QTIRAV KKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKEKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCV+SEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGA+QQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMP+LPINIEDAARSEAEI+KALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQV T+FRQFLL E FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           +ICGDFGRVFEIGPVFRAEDS+THRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFV+MFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 361 LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           +N+ CKK LEA+DKQYPFEPLKYLR TLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

[Close]

BLAST of CSPI03G44420 vs. NCBI nr
Match: XP_022936995.1 (aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita moschata])

HSP 1 Score: 1013.8 bits (2620), Expect = 5.4e-292
Identity = 509/545 (93.39%), Postives = 529/545 (97.06%), Query Frame = 0


Query: 1   MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASS+TPIDEP SA D++SKAVSKKAAKKEAAK EKLRRRQEAAAE+AISSLNVEDDPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKE K + NWTQVGSLT ELKDKYVILRGR+QTIRAV KKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCV+SEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGA+QQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMP+LPINIEDAARSEAEI+KALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQV T+FRQFLL E FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           +ICGDFGRVFEIGPVFRAEDS+THRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFV+MFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 361 LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           +N+ CKK LEA+DKQYPFEPLKYLR TLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FL ERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLTERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

[Close]

BLAST of CSPI03G44420 vs. TAIR 10
Match: AT4G31180.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 741.5 bits (1913), Expect = 4.9e-214
Identity = 381/557 (68.40%), Postives = 445/557 (79.89%), Query Frame = 0


Query: 2   SASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQ---EAAAESAISSLNVEDD 61
           S S   P+    +A ++S +  SKKAAKKEAAK EKLRRRQ   EA   +A  SL  E+D
Sbjct: 3   SESEIPPLSSSTAAAEESGEKTSKKAAKKEAAKLEKLRRRQEQEEATRRTASISLE-END 62

Query: 62  PLSANYGDVPLSDLQS-------KEVKSIE--NWTQVGSLTPELKDKYVILRGRVQTIRA 121
             S NYGDV L++LQS       K ++++E   WT V  L  E+ +  V++RGRV T R 
Sbjct: 63  EFSNNYGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRGRVHTNRP 122

Query: 122 VGKKMAFLVVREKGFTVQCVLSEQPEL-VSRQMVKYVDGLSRESIVDVEGVVSVPNVAIK 181
              K+ F+V+RE G TVQCV+S+  +  V   MVKY+  LSRES VDV GVV++P   + 
Sbjct: 123 TSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDVIGVVTLPKEPLT 182

Query: 182 GASQQVEIQVRKVYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRV 241
           G +QQVEIQVRKVYCI+K++  LP+++EDAARSEA+I+ +LQ      RVNQDTRLNYRV
Sbjct: 183 GTTQQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNYRV 242

Query: 242 LDMRTPANQGIFRIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPA 301
           LD+RTPANQ IF++Q +V   FR+ L  +NFV IHTPKL+ GSSEGG++VFRL+YKGQPA
Sbjct: 243 LDLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEYKGQPA 302

Query: 302 CLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMD 361
           CLAQSPQLHKQMAICGD  RVFE+GPVFRAEDS+THRHLCEF GLDVEMEI+ HYSE+MD
Sbjct: 303 CLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRKHYSEIMD 362

Query: 362 IVDRLFVAMFDSLNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDP 421
           +VD LFV +F SLNE+CKK L+AV KQYPFEPLK+L KTLRLTFEEG+QMLK+AGVE+DP
Sbjct: 363 LVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEVDP 422

Query: 422 LGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEE 481
           LGDLNTE+ERKLGQLVLEKY TEFYILHRYP AVRPFYTM C DNP YSNSFDVFIRGEE
Sbjct: 423 LGDLNTESERKLGQLVLEKYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRGEE 482

Query: 482 IISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLN 541
           IISGAQRVH+PE L +RA  CGIDVKTI TYIDSFRYGAP HGGFGVGLERVVMLFC LN
Sbjct: 483 IISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALN 542

Query: 542 NIRKTSLFPRDPQRIAP 546
           NIRKTSLFPRDPQR++P
Sbjct: 543 NIRKTSLFPRDPQRLSP 558

[Close]

BLAST of CSPI03G44420 vs. TAIR 10
Match: AT4G31180.2 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 741.5 bits (1913), Expect = 4.9e-214
Identity = 381/557 (68.40%), Postives = 445/557 (79.89%), Query Frame = 0


Query: 2   SASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQ---EAAAESAISSLNVEDD 61
           S S   P+    +A ++S +  SKKAAKKEAAK EKLRRRQ   EA   +A  SL  E+D
Sbjct: 3   SESEIPPLSSSTAAAEESGEKTSKKAAKKEAAKLEKLRRRQEQEEATRRTASISLE-END 62

Query: 62  PLSANYGDVPLSDLQS-------KEVKSIE--NWTQVGSLTPELKDKYVILRGRVQTIRA 121
             S NYGDV L++LQS       K ++++E   WT V  L  E+ +  V++RGRV T R 
Sbjct: 63  EFSNNYGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRGRVHTNRP 122

Query: 122 VGKKMAFLVVREKGFTVQCVLSEQPEL-VSRQMVKYVDGLSRESIVDVEGVVSVPNVAIK 181
              K+ F+V+RE G TVQCV+S+  +  V   MVKY+  LSRES VDV GVV++P   + 
Sbjct: 123 TSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDVIGVVTLPKEPLT 182

Query: 182 GASQQVEIQVRKVYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRV 241
           G +QQVEIQVRKVYCI+K++  LP+++EDAARSEA+I+ +LQ      RVNQDTRLNYRV
Sbjct: 183 GTTQQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNYRV 242

Query: 242 LDMRTPANQGIFRIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPA 301
           LD+RTPANQ IF++Q +V   FR+ L  +NFV IHTPKL+ GSSEGG++VFRL+YKGQPA
Sbjct: 243 LDLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEYKGQPA 302

Query: 302 CLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMD 361
           CLAQSPQLHKQMAICGD  RVFE+GPVFRAEDS+THRHLCEF GLDVEMEI+ HYSE+MD
Sbjct: 303 CLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRKHYSEIMD 362

Query: 362 IVDRLFVAMFDSLNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDP 421
           +VD LFV +F SLNE+CKK L+AV KQYPFEPLK+L KTLRLTFEEG+QMLK+AGVE+DP
Sbjct: 363 LVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEVDP 422

Query: 422 LGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEE 481
           LGDLNTE+ERKLGQLVLEKY TEFYILHRYP AVRPFYTM C DNP YSNSFDVFIRGEE
Sbjct: 423 LGDLNTESERKLGQLVLEKYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRGEE 482

Query: 482 IISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLN 541
           IISGAQRVH+PE L +RA  CGIDVKTI TYIDSFRYGAP HGGFGVGLERVVMLFC LN
Sbjct: 483 IISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALN 542

Query: 542 NIRKTSLFPRDPQRIAP 546
           NIRKTSLFPRDPQR++P
Sbjct: 543 NIRKTSLFPRDPQRLSP 558

[Close]

BLAST of CSPI03G44420 vs. TAIR 10
Match: AT4G26870.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 688.3 bits (1775), Expect = 4.9e-198
Identity = 350/531 (65.91%), Postives = 422/531 (79.47%), Query Frame = 0


Query: 17  DDSSKAVSKKAAKKEAAKQEKLRRRQE-AAAESAISSLNVEDDPLSANYGDVPLSDLQSK 76
           ++  + +SKK +KK AAK EKL R+QE   A S+  SL  ED+  S+NYGDV  ++LQS 
Sbjct: 8   EECGEKISKKESKKRAAKLEKLLRKQEREEATSSSLSLEEEDESCSSNYGDVTTNELQSA 67

Query: 77  -EVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVREKGFTVQCVLSEQP 136
            E K +   T V +L  E+    V +RGR+   R VG K+ F+++RE GFTVQCV+ E  
Sbjct: 68  VEGKEL---TDVSNLVEEIVGSEVSIRGRLHKNRLVGTKL-FVILRESGFTVQCVVEE-- 127

Query: 137 ELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRKVYCISKAMPTLPIN 196
             V   M+K+V  LSRES+V++ GVVS P   + G +QQVEI VRK+YC+S+++P LP+ 
Sbjct: 128 TRVGANMIKFVKQLSRESVVELIGVVSHPKKPLTGTTQQVEIHVRKMYCLSRSLPNLPLV 187

Query: 197 IEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIFRIQCQVSTMFRQFL 256
           +EDAARSE++I+K+ + G+Q  RV QDTRLN RVLD+RTPANQ IFRIQCQV   FR++L
Sbjct: 188 VEDAARSESDIEKSGKDGKQAARVLQDTRLNNRVLDIRTPANQAIFRIQCQVQIAFREYL 247

Query: 257 LDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGP 316
             + F+EIHTPKLI GSSEGG++VFRLDYKGQPACLAQSPQLHKQMAICGD  RVFE+GP
Sbjct: 248 QSKGFLEIHTPKLIAGSSEGGSAVFRLDYKGQPACLAQSPQLHKQMAICGDMRRVFEVGP 307

Query: 317 VFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDSLNEKCKKALEAVDK 376
           VFRAEDS+THRHLCEF GLDVEMEI+ HYSE+MD+V  LF  +F  + E+C K LE+V K
Sbjct: 308 VFRAEDSFTHRHLCEFVGLDVEMEIRMHYSEIMDLVGELFPFIFTKIEERCPKELESVRK 367

Query: 377 QYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKLGQLVLEKYGTEFYI 436
           QYPF+ LK+L +TLRLTF EGIQMLK+AG E+DPLGDLNTE+ERKLGQLVLEKY TEFY+
Sbjct: 368 QYPFQSLKFLPQTLRLTFAEGIQMLKEAGEEVDPLGDLNTESERKLGQLVLEKYKTEFYM 427

Query: 437 LHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVK 496
           LHRYP AVRPFYTMP  ++  YSNSFDVFIRGEEI+SGAQR+H PE L +RA+ CGIDVK
Sbjct: 428 LHRYPSAVRPFYTMPYENDSNYSNSFDVFIRGEEIMSGAQRIHDPELLEKRARECGIDVK 487

Query: 497 TIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 546
           TI TYID+FRYGAPPHGGFGVGLERVVML C LNNIRKTSLFPRD QR+ P
Sbjct: 488 TISTYIDAFRYGAPPHGGFGVGLERVVMLLCALNNIRKTSLFPRDSQRLTP 532

[Close]

BLAST of CSPI03G44420 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 125.6 bits (314), Expect = 1.3e-28
Identity = 88/272 (32.35%), Postives = 141/272 (51.84%), Query Frame = 0


Query: 282 DYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKT 341
           D+ G+ A L  S QL  +   C     V+  GP FRAE+S+T RHL EF  ++ E+    
Sbjct: 303 DFFGRQAFLTVSGQLQVETYACA-LSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAF-A 362

Query: 342 HYSEVMDIVDRLFVAMFDSLNEKCKKALEAVDKQYP---FEPLKYLRKTL--RLTFEEGI 401
              + M+  +     M + L EKC   +E + K +     + LK +  T   R+T+ + I
Sbjct: 363 DLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAI 422

Query: 402 QMLKDA---GVEID---PLG-DLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMP 461
           ++L++A   G E D     G DL +E ER L +++ +K      I++ YP  ++ FY M 
Sbjct: 423 ELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKP----LIVYNYPKGIKAFY-MR 482

Query: 462 CYDNPTYSNSFDVFI-RGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAP 521
             D+     + DV + +  E+I G+QR    + + +R +  G+ ++  E Y+D  RYG  
Sbjct: 483 LNDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTV 542

Query: 522 PHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 541
            H GFG+G ER+++   GL+NIR    FPR P
Sbjct: 543 KHCGFGLGFERMILFATGLDNIRDVIPFPRYP 567

[Close]

BLAST of CSPI03G44420 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 124.4 bits (311), Expect = 2.8e-28
Identity = 120/482 (24.90%), Postives = 204/482 (42.32%), Query Frame = 0


Query: 100 LRGRVQTIRAVGKKMAFLVVREKG--FTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVE 159
           + G V+T+R+    + F+ + +      +QCV++   E      V+    L+  S V V+
Sbjct: 114 IMGWVRTLRS-QSSVTFIEINDGSCLSNLQCVMTSDAE--GYDQVESGSILTGAS-VSVQ 173

Query: 160 GVVSVPNVAIKGASQQVEIQVRKVYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVR 219
           G +    VA +G  Q+VE++V K+  + +   + PI                    Q  R
Sbjct: 174 GTI----VASQGTKQKVELKVEKIIVVGECDSSYPI--------------------QKKR 233

Query: 220 VNQDTRLNYRVLDMRTPANQGIFRIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGAS 279
           V+++       L  RT     + R++  ++    +F  +  FV + +P +     EG   
Sbjct: 234 VSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFFQESGFVWVASPIITASDCEGAGE 293

Query: 280 VFRL------------------------------DYKGQPACLAQSPQLHKQMAICGDFG 339
            F +                              D+ G+PA L  S QL+ +        
Sbjct: 294 QFCVTTLIPSSHENTDTSIDAIPKTKGGLIDWSQDFFGKPAFLTVSGQLNGE-TYATALS 353

Query: 340 RVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDSLNEKCKK 399
            V+  GP FRAE+S T RHL EF  ++ E+       + M         +   + + CK+
Sbjct: 354 DVYTFGPTFRAENSNTSRHLAEFWMIEPELAF-ADLDDDMACATAYLQYVVKYVLDNCKE 413

Query: 400 ALEAVDKQYPFEPLKYL-----RKTLRLTFEEGIQMLKDAGVEID-PLG---DLNTEAER 459
            +E  D       ++ L     ++ L+L + + I++L  A  + D P+    DL +E ER
Sbjct: 414 DMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEILLKANKKFDFPVKWGLDLQSEHER 473

Query: 460 KLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHV 519
            + +   E +G    I+  YP  ++ FY     D  T +    +  R  E+I G+QR   
Sbjct: 474 YITE---EAFGGRPVIIRDYPKEIKAFYMRENDDGKTVAAMDMLVPRIGELIGGSQREER 533

Query: 520 PEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPR 541
            E L  R     ++ ++   Y+D  RYG+ PH GFG+G ER+V    G++NIR    FPR
Sbjct: 534 LEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIRDVIPFPR 562

The following BLAST results are available for this feature:

BLAST of CSPI03G44420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M084	6.8e-213	68.40	Aspartate--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=IBI1 PE...	[more]
Q8H104	6.9e-197	65.91	Aspartate--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g268...	[more]
Q5R9I5	2.8e-134	50.00	Aspartate--tRNA ligase, cytoplasmic OS=Pongo abelii OX=9601 GN=DARS1 PE=2 SV=1	[more]
Q922B2	4.8e-134	50.81	Aspartate--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Dars1 PE=1 SV=2	[more]
P14868	6.3e-134	50.00	Aspartate--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=DARS1 PE=1 SV=2	[more]

BLAST of CSPI03G44420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LDS7	2.0e-310	100.00	Aspartyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_3G881580 PE=3 SV=1	[more]
A0A5A7TBL8	7.1e-306	98.53	Aspartyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A1S3CR09	7.1e-306	98.53	Aspartyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503781 PE=3 SV=1	[more]
A0A6J1FFA5	2.6e-292	93.39	Aspartate--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111443419 PE=3 SV=1	[more]
A0A6J1ILA3	3.8e-291	93.21	Aspartate--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111476621 PE=3 SV=1	[more]

BLAST of CSPI03G44420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136326.1	4.2e-310	100.00	aspartate--tRNA ligase 2, cytoplasmic [Cucumis sativus] >KGN60145.1 hypothetical...	[more]
XP_008466345.1	1.5e-305	98.53	PREDICTED: aspartate--tRNA ligase 2, cytoplasmic [Cucumis melo] >KAA0038775.1 as...	[more]
XP_023535979.1	2.4e-292	93.58	aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita pepo subsp. pepo]	[more]
KAG6591612.1	5.4e-292	93.39	Aspartate--tRNA ligase 2, cytoplasmic, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022936995.1	5.4e-292	93.39	aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita moschata]	[more]

BLAST of CSPI03G44420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31180.1	4.9e-214	68.40	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT4G31180.2	4.9e-214	68.40	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT4G26870.1	4.9e-198	65.91	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT5G56680.1	1.3e-28	32.35	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT4G17300.1	2.8e-28	24.90	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 25..47
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 192..545 e-value: 7.1E-130 score: 435.2
None	No IPR available	GENE3D	2.40.50.140		coord: 56..190 e-value: 1.1E-39 score: 137.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 14..44
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..50
None	No IPR available	PANTHER	PTHR43450:SF1	ASPARTATE--TRNA LIGASE, CYTOPLASMIC	coord: 16..545
None	No IPR available	CDD	cd04320	AspRS_cyto_N	coord: 97..197 e-value: 9.07077E-45 score: 151.562
None	No IPR available	CDD	cd00776	AsxRS_core	coord: 217..541 e-value: 1.63904E-166 score: 473.206
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 215..544
IPR002312	Aspartyl/Asparaginyl-tRNA synthetase, class IIb	PRINTS	PR01042	TRNASYNTHASP	coord: 290..302 score: 55.24 coord: 307..320 score: 51.79 coord: 500..514 score: 55.15 coord: 460..476 score: 40.11
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 220..539 e-value: 1.1E-76 score: 258.0
IPR004365	OB-fold nucleic acid binding domain, AA-tRNA synthetase-type	PFAM	PF01336	tRNA_anti-codon	coord: 98..182 e-value: 1.1E-7 score: 31.8
IPR004523	Aspartate-tRNA synthetase, type 2	TIGRFAM	TIGR00458	TIGR00458	coord: 89..545 e-value: 9.8E-144 score: 477.4
IPR004523	Aspartate-tRNA synthetase, type 2	PANTHER	PTHR43450	ASPARTYL-TRNA SYNTHETASE	coord: 16..545
IPR004523	Aspartate-tRNA synthetase, type 2	HAMAP	MF_02075	Asp_tRNA_synth_type2	coord: 81..545 score: 39.832504
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 240..537 score: 19.137009
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 62..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G44420.1	CSPI03G44420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006422	aspartyl-tRNA aminoacylation
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0017101	aminoacyl-tRNA synthetase multienzyme complex
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004815	aspartate-tRNA ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0000166	nucleotide binding