CSPI03G42650 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G42650
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	CRAL-TRIO domain-containing protein
Location	Chr3: 36649730 .. 36655044 (+)
RNA-Seq Expression	CSPI03G42650
Synteny	CSPI03G42650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAGTTGGTGCGTGACAAAGATGAAGGAGTTGGTAGAGTTAATTTTATTTAAGAGAAGAGTTCGTAAATATTTAGTAGAGGGAAAGGGTAGCTTCTTGGAATCCACGAAATAAGTTGGAGCAAAATAAATATTAAGAGGAAAAGGAAAAAGCATCTAACGTCTAAACAAAACAAAAAAGAAGAGAAAAAGAACAAAGGAAGCAAGAAGAAAGAGAGGAAGGAGCTCGAAAGAAGAAGGGGGTGGGAGAAGGAAAGTCAACCACCCGTCGCTGAAAATTGGTTGGGAAAGTGAAAACTAACGCCGTAGTCGGTTTGAAGAAGCTTTTACAGAGGGATCCAACTTGGTAAGCCATTTTCTTATCAGGTGAAGCTTGGAAAATGGAGATCTTGGATGGTAGATTTATGGGTTTTCTTTCTTTTGATAAGTTTATTGATATTTCTTCCTTTATGTCTAAATAATTTCTTTGAAGTTTGTATTCTTTCTTTGACGCAAGAAATTTTTATTGATCTTGTGAGTATTTTGATTGAGATCAGCTCAAGGACAAGAAGAATAGGAAAGTGGTAACCCACTTTTGAGGGTGAAAAAAAAGTGGGAAGAAAAAAAGGGGGAATTAATTAAATGAAGAAAAATGTGAGTATTGAGGAAACAAGAGAATGTGTTTTAATTTCTTCTTCTTTTCCACTATGTTCCTTCTTGGAGTTTATATGATAATACTTATAAATAAGAGGAGTGTTGGAATGAAAGAAGTAAAGGGGAAGAAGAATGAAAAAGAAATTTGTTGTAGATAGAAGAAAAAGAAGAGGAAGAAGAAGATGATGAATTTAATAATTGTAGTTTTTAGACAATGGATTTAATTTTTGAGAAACTACAACTTTTCATTTAAGAGGGAGAGAAATGATGAAAATAAATGGAAAAAACATATAATCATAGCATGGAAGGGAAGAAAAGGAAATGTATCAAATGGGGAGGAAATTCTAGCTTTATGAGGGAATAAGTAATCTTTATAAACAAAATAAAAATAAAGGTATACAAAAGTTACTAGAGAAAGAATGAAGATAAGTCATGAGGAATGAGTTTGAGTTTAGAATTCATATAAGTTCATGGTTGGTCAATGGTTATTGATCTATTTATTGAAATTTCAGGTTGGACTGAAATTTGAATCCCTTTTCAACCGAGGAGTTATCGCCGGCATAATTGTGAGTGATTATTATGCAAATAATTTTCCTATTTTTGCAAATTATGGTTTTTGATTTTGGAAATTGATTATGATTTATGTTCATACCATCGATTTGGGTATTCAAGATTTCAAGAAACTGAACCACTCATTCTGCAATTGTTTACAACTGGAAAGAGACTCACGCATTTATGCTTCTTGGTTTAGAATAATTGAATTTCAAGGAATGTGTGTTTAAGTGTTTTTTGTTTGTTTGTTTGTTTGTTAAGAGATGTATGTGTAAGTGCGATTATAGACATAACGACTTACTTGTATCATTGGAGCTTATGGAAGGTAAATTGTTGTAATCTATTGATTTTAGTCATTTGGCAAAAGTTTTCAGTTATTTGACAAGAAATCAAGTTTCTCTGACTTTTAGAAATGAGGAAATATGTTTTATGATTATTGGAATATTTAGAAATCAAGTTTCTGTCTTTTAGAAGGGTTTTACGGTTACTAGAAAAATAATTAAGTTGCAAAATAAGGTTTCTTTCAAGAGAGTATGATTTCCAATACGATTTGAGATTGATGAGAAAGTATGGTTTTATAATGCTTTAAGAATCATGATAATTTAAAATTTTCAGAATTGATGAGATTTATGTATTTGGAGTGAAAAGTGGTTTCGATCCACTAGATTGATGAACCTTGTGGTGATTACTTGTATGCCATGTGAATGTGTTTCTACAGTTTATACTAGTGTGCCTAGGCAGGGAATGTTTCGTGGGCATTTACGGAAAGGTTTTGCTCTGCCACGTGTCTATGATAGATTGAGAGTTTTACTTCATGCATAGCATGATAGATTGCTCTATTGTGCATGCTGAGACTATTCTAAGCCAGTATGATAGAGATTTGGGTATAGATTTGGTGATAGTTTTTGCCAGTTTACGTTTATGTTTTCAAGTGGTACACAACTCATTCATTTATGGTGGAGTTTTGAGTTTGAAACAAAATATTTGAAAGTTGTTTCTTTTCAGGTGTGGTTTATTCGATTTTTAGAGGAAACTCTACTGAAATTTTAAGACTTTCATTTTGTAAAGTTTATTTTTAACAGTGTTTGCAATTAAAAGTTTCACAAGTCATTTGTTTTAAAAAATGTTAGTTTGAGATTGACAATGCGTTTCATGGTTTAGCTGGAATTTAATATTGACATGCCACCCATGTTTTAAAAGGCTAAAGGCGGCCTTTGAGGCTTTTCCTCTCCAAGAGGCGAGGCCTAAGCCTCAAATAATTACTTAAATACATTTTACTAAACCTAAATTGGTATGATCAGTATTGCTAAACATGATAGCCATATAAAAGTGTAAAAAGAAAGTTCACACCCACAATTTTCACTTAGTGAAAAAATTCTAAAAGGACATCTTCATCATCATTAATACTGCTATTATTAGTACAGGGTTGAATTATACCATATATGTTTGCTTTCAACATTTTTTAAGTTCACAAGAGTGAAAAGAGTCCTAGTTCAAATTGTCACAGCTCAAACCTTAATATTTTTGAAGAATTAGACAAATCACGATTATTTTTCTAATCACTAGGCAAGGCATAGAGATTTGAACCTTAGTGCATTTTCTCAAAGGCGTAAGCCTCTTATGGTTCAAGAAAGGTGTAAGTCTTGAAGCTAACACTTGGAGCCTTGCGAACCTCAACGCGTAAGCCTCAATAGCTTTTTAAAACATTGCAAGCCACGCCCTAGGTAATGTAGGGTAATATGGGGTGTTGTGTTACAGGAGACGAGCTAGATTTTGATTCTGCACATTGACTTGTATAAGAATCTTGCTTGAGGTTATTTGTTTTTAAAATTTTTATTTTGATCTTCTAGTGCCATGTTCTCTAATTGATCAACATCTGAGATCCATCACTGTTTGATTGTTGCATTTGACTCTTGATGTTGAAAATTTGCTTACCTTTCAGTTAAGCCTAATTATTTGGAATTTGCTGGCGATTTTTGGTGGTAATTCCTTTCTACTGCTTTGGAAGTCTTCCATCGGTAAATTACCAACGTTTTTGCTAGGAAGACTCTGGCTTCATCTTCCAGCAAGTTCATATCGAACTTTAGGCTATTCCTTTTTACAAGGAAGCAGAGAATTTATGTTAGTTGGAGTGTAACAGGTTCGTGATTGGCAGCTACAATGTTTGGCCGACGGTCTACTTCTCATCACGAGGAAGAGAAAAATCCAGAACAGATCACATCGAAGGTATGAATCCAATTGAGTTGATTATAACAAAACGTATGTAGACTTCTCTGTTAGTTTCCGGATCAAATAGTTATAAACTTTTAACTTGAATGAGTCCGATTGGATCTAAACCATCTTAAAGATTTTTCAGGATCATGATTCATGTGTTTTACAACAGGTTTTAAGATGATTGGTAATCATAGATAATAAGATTTTACGATTTTGCAGGTCAATGAGCTAAAGGCTAAGTTAGGACCCATAGTTGGGCGCAATGCGATATATTGCAGTGATGCATGTTTGAAAAGATATCTAGTGGCCAGGAATTGGAATGTAGACAAAGCAAAGAAAATGCTGGAAGAGACGTTCAAATGGAGATCTATTTATAAGCCTGAAGAAATTCGTTGGGTAAGCTTGTAGTAGTTTCTTTTTTCTTTTTGTTTGAAAAGGGCTTTTTAAGTACAACATGGGTAGAGAGATTTGAATCCGAGATCTCTTGTCTTTGGGAAAAAATAGATGTCAGTTAAGCTAGACTCATGTTAGCACTTGTAGTAGTTTCATGAAGCTTGACACTAATGAAACTCAAGGTGAAGTCATGAAGAGTTGAATAGTAATGAAACCCAAGGTGAAGTCATGAAGAATTGAATAGTAGTAGTGATGGAGTTTTATGTTGGCTCTAAAACTAATCATTTAGTTTGTCTTCATTTTAGCCTGAAATAGCATTTGAAAGTGAGACCGGAAAACTTTACCGAGCAAGTTTTCATGACCGAGAAGGAAGAACTGTTCTTATAATGAAGCCAGGAAAGCAGGTATTTTATTTGGGTAAATTTGCTAAAATTCTTATTATTTCCGGTTTTTGCTATTTTTCAGTTTGCTGTATCAGAAATTTCTATTTCCACGGCTTTCTCTAACAGTTAGAAGCCTAATATTTTTTCTTACTATTTTACAGAACACGACATCATTAGAGAATCAGATTCGGCATTTGGTCTATCTTATGGAGAATGCTCTTCTTAACCTTCCTGAAGGTCAGGAGCAGATGTCATGGCTGATTGATTTTAATGGTTGGTCTCTTAGCACCAGTGTGCCAATCAAATCTGCACGGGAGACCGTTAACATCTTGCAGAACCACTATCCAGAGAGATTAGCCCTAGCGTTTCTATATAATCCCCCAAGGATATTTGAAGCATTCTGGAAGGTATGGTATATACTTGCAATATCTCTTCCATCCTTATGGTAATAGCTACCAATAAAACAAGCACATTATGGTATTATTTTGTTTACTACTTGTAGGTAGTCAAGTACCTTTTAGATCCGAAGACATTTCAGAAAGTGAGATTTGTTTATCCTAAGAAGCAAGAGAGTGTGGAACTCATGAAGTCATATTTTGATGAGGAGAATCTTCCATCAGAGTTTGGAGGAAAAGCCCAACTGGAATATGTTCATGAGGAGTTTTCTACATTAATGATTCAAGATGATATTAAATGTGCTGCCTTCTGGGAGCAGGGTGAAAAGCAACACCATATTGTCAATGGCTACTCAAGTGCAGTGGTGGCTCCAGAATGAGTGTACTTTTTAGCAACATATCAGTTACAGGGATTGCCACTTGTATTTGCGGCTGACACCCTTGAAATGTGTTTCATTTATGAACTTTTCATGGTCTACAGAAGATATAGAGCAAAAAGGTTTGATGTAAGCCCTTAATTTCACTGATCTTGCGTAATTACACATGATGGAGAGATTCTGTTCTAATTGGTAATTGTAAGTTTGTAACTTTGGTTAGGAGACTTTGAATTATCCTGTAAAAGATTGGTCGATCCAATCGACTTATAGAGATCCAACTATCCAAAAAATAATCTTCATTTCTGTTACATCTCAAAAACAGATTATTCACAAGAAGCCAAGAGTGATCCAGTCATTTGATAAGGAAAT

mRNA sequence

AAGAGTTGGTGCGTGACAAAGATGAAGGAGTTGGTAGAGTTAATTTTATTTAAGAGAAGAGTTCGTAAATATTTAGTAGAGGGAAAGGGTAGCTTCTTGGAATCCACGAAATAAGTTGGAGCAAAATAAATATTAAGAGGAAAAGGAAAAAGCATCTAACGTCTAAACAAAACAAAAAAGAAGAGAAAAAGAACAAAGGAAGCAAGAAGAAAGAGAGGAAGGAGCTCGAAAGAAGAAGGGGGTGGGAGAAGGAAAGTCAACCACCCGTCGCTGAAAATTGGTTGGGAAAGTGAAAACTAACGCCGTAGTCGGTTTGAAGAAGCTTTTACAGAGGGATCCAACTTGGTTGGACTGAAATTTGAATCCCTTTTCAACCGAGGAGTTATCGCCGGCATAATTTTAAGCCTAATTATTTGGAATTTGCTGGCGATTTTTGGTGGTAATTCCTTTCTACTGCTTTGGAAGTCTTCCATCGGTAAATTACCAACGTTTTTGCTAGGAAGACTCTGGCTTCATCTTCCAGCAAGTTCATATCGAACTTTAGGCTATTCCTTTTTACAAGGAAGCAGAGAATTTATGTTAGTTGGAGTGTAACAGGTTCGTGATTGGCAGCTACAATGTTTGGCCGACGGTCTACTTCTCATCACGAGGAAGAGAAAAATCCAGAACAGATCACATCGAAGGTCAATGAGCTAAAGGCTAAGTTAGGACCCATAGTTGGGCGCAATGCGATATATTGCAGTGATGCATGTTTGAAAAGATATCTAGTGGCCAGGAATTGGAATGTAGACAAAGCAAAGAAAATGCTGGAAGAGACGTTCAAATGGAGATCTATTTATAAGCCTGAAGAAATTCGTTGGCCTGAAATAGCATTTGAAAGTGAGACCGGAAAACTTTACCGAGCAAGTTTTCATGACCGAGAAGGAAGAACTGTTCTTATAATGAAGCCAGGAAAGCAGAACACGACATCATTAGAGAATCAGATTCGGCATTTGGTCTATCTTATGGAGAATGCTCTTCTTAACCTTCCTGAAGGTCAGGAGCAGATGTCATGGCTGATTGATTTTAATGGTTGGTCTCTTAGCACCAGTGTGCCAATCAAATCTGCACGGGAGACCGTTAACATCTTGCAGAACCACTATCCAGAGAGATTAGCCCTAGCGTTTCTATATAATCCCCCAAGGATATTTGAAGCATTCTGGAAGGTAGTCAAGTACCTTTTAGATCCGAAGACATTTCAGAAAGTGAGATTTGTTTATCCTAAGAAGCAAGAGAGTGTGGAACTCATGAAGTCATATTTTGATGAGGAGAATCTTCCATCAGAGTTTGGAGGAAAAGCCCAACTGGAATATGTTCATGAGGAGTTTTCTACATTAATGATTCAAGATGATATTAAATGTGCTGCCTTCTGGGAGCAGGGTGAAAAGCAACACCATATTGTCAATGGCTACTCAAGTGCAGTGGTGGCTCCAGAATGAGTGTACTTTTTAGCAACATATCAGTTACAGGGATTGCCACTTGTATTTGCGGCTGACACCCTTGAAATGTGTTTCATTTATGAACTTTTCATGGTCTACAGAAGATATAGAGCAAAAAGGTTTGATGTAAGCCCTTAATTTCACTGATCTTGCGTAATTACACATGATGGAGAGATTCTGTTCTAATTGGTAATTGTAAGTTTGTAACTTTGGTTAGGAGACTTTGAATTATCCTGTAAAAGATTGGTCGATCCAATCGACTTATAGAGATCCAACTATCCAAAAAATAATCTTCATTTCTGTTACATCTCAAAAACAGATTATTCACAAGAAGCCAAGAGTGATCCAGTCATTTGATAAGGAAAT

Coding sequence (CDS)

ATGTTTGGCCGACGGTCTACTTCTCATCACGAGGAAGAGAAAAATCCAGAACAGATCACATCGAAGGTCAATGAGCTAAAGGCTAAGTTAGGACCCATAGTTGGGCGCAATGCGATATATTGCAGTGATGCATGTTTGAAAAGATATCTAGTGGCCAGGAATTGGAATGTAGACAAAGCAAAGAAAATGCTGGAAGAGACGTTCAAATGGAGATCTATTTATAAGCCTGAAGAAATTCGTTGGCCTGAAATAGCATTTGAAAGTGAGACCGGAAAACTTTACCGAGCAAGTTTTCATGACCGAGAAGGAAGAACTGTTCTTATAATGAAGCCAGGAAAGCAGAACACGACATCATTAGAGAATCAGATTCGGCATTTGGTCTATCTTATGGAGAATGCTCTTCTTAACCTTCCTGAAGGTCAGGAGCAGATGTCATGGCTGATTGATTTTAATGGTTGGTCTCTTAGCACCAGTGTGCCAATCAAATCTGCACGGGAGACCGTTAACATCTTGCAGAACCACTATCCAGAGAGATTAGCCCTAGCGTTTCTATATAATCCCCCAAGGATATTTGAAGCATTCTGGAAGGTAGTCAAGTACCTTTTAGATCCGAAGACATTTCAGAAAGTGAGATTTGTTTATCCTAAGAAGCAAGAGAGTGTGGAACTCATGAAGTCATATTTTGATGAGGAGAATCTTCCATCAGAGTTTGGAGGAAAAGCCCAACTGGAATATGTTCATGAGGAGTTTTCTACATTAATGATTCAAGATGATATTAAATGTGCTGCCTTCTGGGAGCAGGGTGAAAAGCAACACCATATTGTCAATGGCTACTCAAGTGCAGTGGTGGCTCCAGAATGA

Protein sequence

MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKAKKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLENQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLALAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE*

Homology

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BLAST of CSPI03G42650 vs. ExPASy Swiss-Prot
Match: Q9UU99 (CRAL-TRIO domain-containing protein C23B6.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC23B6.04c PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.7e-29
Identity = 80/254 (31.50%), Postives = 131/254 (51.57%), Query Frame = 0


Query: 11  EEEKNPEQITSKVNELK--------AKLGPIVGRNAIYCSDACLKRYLVARNWNVDKAKK 70
           E+E+  E++     ELK        +K   ++    ++ +  C+ RYL A  W+V  AKK
Sbjct: 595 EQEEMYEEVLKYCLELKEIPVASNSSKKTDLIELERLWLTRECILRYLRATKWHVSNAKK 654

Query: 71  MLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLENQ 130
            + +T  WR  +    +   EI  E+ TGK     + D++GR  L + P +QNT +   Q
Sbjct: 655 RIVDTLVWRRHFGVNNMDPDEIQEENATGKQVLLGY-DKDGRPCLYLYPARQNTKTSPLQ 714

Query: 131 IRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLALA 190
           IRHLV+ +E A+  +P G E ++ LI+F   S  ++  +   +E +NILQ HY ERL  A
Sbjct: 715 IRHLVFSLECAIDLMPPGVETLALLINFKSSSNRSNPSVGQGKEVLNILQTHYCERLGRA 774

Query: 191 FLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQ 250
            + N P     F+K++   +DP T +K++F  P        +  Y  ++ L S FGG   
Sbjct: 775 LVINIPWAVWGFFKLISPFIDPITREKLKFNEP--------LDRYVPKDQLDSNFGGSLH 834

Query: 251 LEYVHEEFSTLMIQ 257
            EY HE++   +++
Sbjct: 835 FEYHHEKYWPQLVE 839

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BLAST of CSPI03G42650 vs. ExPASy Swiss-Prot
Match: P53844 (Phosphatidylinositol transfer protein PDR17 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PDR17 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 3.9e-26
Identity = 86/268 (32.09%), Postives = 128/268 (47.76%), Query Frame = 0


Query: 11  EEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKAKKMLEETFKW 70
           ++ K PE +    N   A   P+      + S  C  RYL A  WN   A K L +T  W
Sbjct: 60  DDLKLPENLNDLDNGTHANDRPLSDWEKFWLSRECFLRYLRANKWNTANAIKGLTKTLVW 119

Query: 71  R---SIYKPEEIRWP----EIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLENQI 130
           R    +   +E + P    ++A E+ETGK     F D   R +  MK G+QNT S   Q+
Sbjct: 120 RREIGLTHGKEDKDPLTADKVAVENETGKQVILGF-DNAKRPLYYMKNGRQNTESSFRQV 179

Query: 131 RHLVYLMENALLNLPEGQEQMSWLIDFNGWS-----LSTSVPIKSARETVNILQNHYPER 190
           + LVY+ME A    P+G E+++ L+DF  +         + PI  AR  +N++Q+HYPER
Sbjct: 180 QELVYMMETATTVAPQGVEKITVLVDFKSYKEPGIITDKAPPISIARMCLNVMQDHYPER 239

Query: 191 LALAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFG 250
           LA   L N P    AF K++   LDP T  K  F  P         +++ +   L + + 
Sbjct: 240 LAKCVLINIPWFAWAFLKMMYPFLDPATKAKAIFDEP--------FENHIEPSQLDALYN 299

Query: 251 GKAQLEYVHEEFSTLMIQ--DDIKCAAF 265
           G    +Y HE +   M++  DD++   F
Sbjct: 300 GLLDFKYKHEVYWPDMVKKVDDLRLKRF 318

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BLAST of CSPI03G42650 vs. ExPASy Swiss-Prot
Match: Q55CU8 (Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=pitC PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 5.7e-25
Identity = 68/235 (28.94%), Postives = 125/235 (53.19%), Query Frame = 0


Query: 25  ELKAKLGPIVGRNAI-YCSDACLKRYLVARNWNVDKAKKMLEETFKWRSIYKPEEIRWPE 84
           E+K+    +     I +C+D C  RYL ARN+ V K++KML +T +WR  ++P++I+   
Sbjct: 54  EIKSNFSDLTDPTDIAFCTDMCFLRYLRARNYIVSKSEKMLRDTLEWRKKFRPQDIQLGG 113

Query: 85  IAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLENQI--RHLVYLMENAL--LNLPE 144
              E  +      +  D++GR ++   P      ++ +++  ++LVY +E     ++ P+
Sbjct: 114 DIREIGSAGCVYVNKRDKKGRPIIFAVPRNDTLKNVPSELKFKNLVYWLEQGFSRMDEPK 173

Query: 145 GQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLALAFLYNPPRIFEAFWKVVK 204
           G EQ  +++D+  +  S ++ +K+  E ++ L +H PER+  +   +PP +F   WK++ 
Sbjct: 174 GIEQFCFIVDYKDFG-SGNMDMKTNLEAMHFLLDHCPERMGQSLFLDPPALFWFAWKIIS 233

Query: 205 YLLDPKTFQKVRFVYPKKQES----VELMKSYFDEENLPSEFGGKAQLEYVHEEF 251
             L+  T  KVRF+  KK +      EL++ Y D ENL    GG     Y  +E+
Sbjct: 234 PFLNEVTLSKVRFINSKKVDGKRTFAELLE-YVDIENLEQNLGGNLDYNYNIDEY 286

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BLAST of CSPI03G42650 vs. ExPASy Swiss-Prot
Match: P53860 (Phosphatidylinositol transfer protein PDR16 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PDR16 PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.8e-24
Identity = 72/252 (28.57%), Postives = 122/252 (48.41%), Query Frame = 0


Query: 20  TSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKAKKMLEETFKWRSIY----- 79
           TS+ N+ +  L P+      + +  C  RYL A  W +      +  T  WR  +     
Sbjct: 64  TSEKNKSEDDLKPLEEEEKAWLTRECFLRYLRATKWVLKDCIDRITMTLAWRREFGISHL 123

Query: 80  ---KPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLENQIRHLVYLME 139
                ++I    +A E+E+GK     + + + R +L +KPG+QNT +   Q++HLV+++E
Sbjct: 124 GEEHGDKITADLVAVENESGKQVILGY-ENDARPILYLKPGRQNTKTSHRQVQHLVFMLE 183

Query: 140 NALLNLPEGQEQMSWLIDFNGW-------SLSTSVPIKSARETVNILQNHYPERLALAFL 199
             +  +P GQ+ ++ LIDF  +         S   PI   +E ++ILQ HYPERL  A L
Sbjct: 184 RVIDFMPAGQDSLALLIDFKDYPDVPKVPGNSKIPPIGVGKEVLHILQTHYPERLGKALL 243

Query: 200 YNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQLE 257
            N P +   F K++   +DP T +K+ F  P           Y  +  L S +GG  + +
Sbjct: 244 TNIPWLAWTFLKLIHPFIDPLTREKLVFDEP--------FVKYVPKNELDSLYGGDLKFK 303

[Close]

BLAST of CSPI03G42650 vs. ExPASy Swiss-Prot
Match: P53989 (SEC14 cytosolic factor OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=SEC14 PE=3 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 4.3e-17
Identity = 71/221 (32.13%), Postives = 112/221 (50.68%), Query Frame = 0


Query: 43  DACLKRYLVARNWNVDKAKKMLEETFKWRSIYKPEEIRWPEIAFESE--TGKLYRASFH- 102
           D+ L R+L AR ++V  AK+M E   KWR  Y    I   +  ++ +    K Y   +H 
Sbjct: 53  DSTLLRFLRARKFDVALAKEMFENCEKWRKEYGTNTI-MQDFHYDEKPLVAKYYPQYYHK 112

Query: 103 -DREGRTVLIMKPGKQNTTSL------ENQIRHLVYLMENAL-LNLPEGQEQMSWLI--- 162
            D++GR V   + G  N T +      E  +++LV+  E+ +   LP       +L+   
Sbjct: 113 TDKDGRPVYFEELGAVNLTEMEKITTQERMLKNLVWEYESVVNYRLPACSRAAGYLVETS 172

Query: 163 ----DFNGWSLSTSVPIKS-ARETVNILQNHYPERLALAFLYNPPRIFEAFWKVVKYLLD 222
               D  G S+S++  + S  RE   I QN+YPER+   +L N P  F   +++ K  LD
Sbjct: 173 CTVMDLKGISISSAYSVLSYVREASYISQNYYPERMGKFYLINAPFGFSTAFRLFKPFLD 232

Query: 223 PKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQLE 245
           P T  K+ F+     +S EL+K     ENLPS+FGGK++++
Sbjct: 233 PVTVSKI-FILGSSYQS-ELLKQ-IPAENLPSKFGGKSEVD 269

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BLAST of CSPI03G42650 vs. ExPASy TrEMBL
Match: A0A1S3CRQ6 (CRAL-TRIO domain-containing protein C23B6.04c OS=Cucumis melo OX=3656 GN=LOC103503951 PE=4 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 1.8e-162
Identity = 282/286 (98.60%), Postives = 283/286 (98.95%), Query Frame = 0


Query: 1   MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA 60
           MFGRRSTSHHEEE NPEQITSKVNELKAKLGP+VGRNAIYCSDACLKRYLVARNWNVDKA
Sbjct: 1   MFGRRSTSHHEEENNPEQITSKVNELKAKLGPLVGRNAIYCSDACLKRYLVARNWNVDKA 60

Query: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120
           KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE
Sbjct: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA
Sbjct: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240

Query: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE 287
           AQLEYVHEEFSTLMIQDDIKCAAFWEQ EKQHH VNGYSSAVVAPE
Sbjct: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQDEKQHHTVNGYSSAVVAPE 286

[Close]

BLAST of CSPI03G42650 vs. ExPASy TrEMBL
Match: A0A5D3D813 (CRAL-TRIO domain-containing protein C23B6.04c OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold242G00200 PE=4 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 1.8e-162
Identity = 282/286 (98.60%), Postives = 283/286 (98.95%), Query Frame = 0


Query: 1   MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA 60
           MFGRRSTSHHEEE NPEQITSKVNELKAKLGP+VGRNAIYCSDACLKRYLVARNWNVDKA
Sbjct: 1   MFGRRSTSHHEEENNPEQITSKVNELKAKLGPLVGRNAIYCSDACLKRYLVARNWNVDKA 60

Query: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120
           KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE
Sbjct: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA
Sbjct: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240

Query: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE 287
           AQLEYVHEEFSTLMIQDDIKCAAFWEQ EKQHH VNGYSSAVVAPE
Sbjct: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQDEKQHHTVNGYSSAVVAPE 286

[Close]

BLAST of CSPI03G42650 vs. ExPASy TrEMBL
Match: A0A6J1J0L2 (CRAL-TRIO domain-containing protein C23B6.04c isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480825 PE=4 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.1e-156
Identity = 270/286 (94.41%), Postives = 278/286 (97.20%), Query Frame = 0


Query: 1   MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA 60
           MFGR STSHHEE  + EQITSKVNELKAKLGP+VGRNAIYCSDACLKRYLVARNWNVDKA
Sbjct: 1   MFGRWSTSHHEEGNSQEQITSKVNELKAKLGPLVGRNAIYCSDACLKRYLVARNWNVDKA 60

Query: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120
           KKMLEETFKWRS+YKP+EIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE
Sbjct: 61  KKMLEETFKWRSMYKPDEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA
Sbjct: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKY LDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 181 LAFLYNPPRIFEAFWKVVKYFLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240

Query: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE 287
           AQLEYVHEE+S LMIQDD+KCAAFWE GEKQHH+VNGY+SA VAPE
Sbjct: 241 AQLEYVHEEYSRLMIQDDVKCAAFWEPGEKQHHVVNGYTSAAVAPE 286

[Close]

BLAST of CSPI03G42650 vs. ExPASy TrEMBL
Match: A0A6J1FJV0 (CRAL-TRIO domain-containing protein C23B6.04c isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446094 PE=4 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.4e-156
Identity = 269/286 (94.06%), Postives = 278/286 (97.20%), Query Frame = 0


Query: 1   MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA 60
           MFGR STSHHEE  + EQITSKVNELKAKLGP+VGRNAIYCSDACLKRYLVARNWNVDKA
Sbjct: 1   MFGRWSTSHHEEGNSQEQITSKVNELKAKLGPLVGRNAIYCSDACLKRYLVARNWNVDKA 60

Query: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120
           KKMLEETFKWRS+YKP+EIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE
Sbjct: 61  KKMLEETFKWRSMYKPDEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA
Sbjct: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKY LDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 181 LAFLYNPPRIFEAFWKVVKYFLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240

Query: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE 287
           AQLEYVHEE+S LMIQDD+KCAAFWE GEKQHH++NGY+SA VAPE
Sbjct: 241 AQLEYVHEEYSRLMIQDDVKCAAFWEPGEKQHHVINGYTSAAVAPE 286

[Close]

BLAST of CSPI03G42650 vs. ExPASy TrEMBL
Match: A0A6J1IUY5 (CRAL-TRIO domain-containing protein C23B6.04c isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111480825 PE=4 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 1.6e-147
Identity = 253/265 (95.47%), Postives = 261/265 (98.49%), Query Frame = 0


Query: 22  KVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKAKKMLEETFKWRSIYKPEEIRW 81
           +VNELKAKLGP+VGRNAIYCSDACLKRYLVARNWNVDKAKKMLEETFKWRS+YKP+EIRW
Sbjct: 21  QVNELKAKLGPLVGRNAIYCSDACLKRYLVARNWNVDKAKKMLEETFKWRSMYKPDEIRW 80

Query: 82  PEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLENQIRHLVYLMENALLNLPEGQ 141
           PEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLENQIRHLVYLMENALLNLPEGQ
Sbjct: 81  PEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLENQIRHLVYLMENALLNLPEGQ 140

Query: 142 EQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLALAFLYNPPRIFEAFWKVVKYL 201
           EQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLALAFLYNPPRIFEAFWKVVKY 
Sbjct: 141 EQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLALAFLYNPPRIFEAFWKVVKYF 200

Query: 202 LDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQLEYVHEEFSTLMIQDDIKC 261
           LDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQLEYVHEE+S LMIQDD+KC
Sbjct: 201 LDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQLEYVHEEYSRLMIQDDVKC 260

Query: 262 AAFWEQGEKQHHIVNGYSSAVVAPE 287
           AAFWE GEKQHH+VNGY+SA VAPE
Sbjct: 261 AAFWEPGEKQHHVVNGYTSAAVAPE 285

[Close]

BLAST of CSPI03G42650 vs. NCBI nr
Match: XP_031738483.1 (CRAL-TRIO domain-containing protein C23B6.04c isoform X1 [Cucumis sativus])

HSP 1 Score: 589.0 bits (1517), Expect = 2.3e-164
Identity = 286/286 (100.00%), Postives = 286/286 (100.00%), Query Frame = 0


Query: 1   MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA 60
           MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA
Sbjct: 50  MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA 109

Query: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120
           KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE
Sbjct: 110 KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 169

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA
Sbjct: 170 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 229

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 230 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 289

Query: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE 287
           AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE
Sbjct: 290 AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE 335

[Close]

BLAST of CSPI03G42650 vs. NCBI nr
Match: XP_031738484.1 (CRAL-TRIO domain-containing protein C23B6.04c isoform X2 [Cucumis sativus])

HSP 1 Score: 589.0 bits (1517), Expect = 2.3e-164
Identity = 286/286 (100.00%), Postives = 286/286 (100.00%), Query Frame = 0


Query: 1   MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA 60
           MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA
Sbjct: 28  MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA 87

Query: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120
           KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE
Sbjct: 88  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 147

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA
Sbjct: 148 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 207

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 208 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 267

Query: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE 287
           AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE
Sbjct: 268 AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE 313

[Close]

BLAST of CSPI03G42650 vs. NCBI nr
Match: XP_004147786.1 (CRAL-TRIO domain-containing protein C23B6.04c isoform X3 [Cucumis sativus] >XP_011652412.1 CRAL-TRIO domain-containing protein C23B6.04c isoform X3 [Cucumis sativus] >XP_031738485.1 CRAL-TRIO domain-containing protein C23B6.04c isoform X3 [Cucumis sativus] >XP_031738486.1 CRAL-TRIO domain-containing protein C23B6.04c isoform X3 [Cucumis sativus] >XP_031738487.1 CRAL-TRIO domain-containing protein C23B6.04c isoform X3 [Cucumis sativus] >KAE8651327.1 hypothetical protein Csa_001508 [Cucumis sativus])

HSP 1 Score: 589.0 bits (1517), Expect = 2.3e-164
Identity = 286/286 (100.00%), Postives = 286/286 (100.00%), Query Frame = 0


Query: 1   MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA 60
           MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA
Sbjct: 1   MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA 60

Query: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120
           KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE
Sbjct: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA
Sbjct: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240

Query: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE 287
           AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE
Sbjct: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE 286

[Close]

BLAST of CSPI03G42650 vs. NCBI nr
Match: XP_008466570.1 (PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c [Cucumis melo] >XP_008466571.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c [Cucumis melo] >XP_008466572.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c [Cucumis melo] >XP_008466573.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c [Cucumis melo] >KAA0041624.1 CRAL-TRIO domain-containing protein C23B6.04c [Cucumis melo var. makuwa] >TYK19633.1 CRAL-TRIO domain-containing protein C23B6.04c [Cucumis melo var. makuwa])

HSP 1 Score: 581.6 bits (1498), Expect = 3.6e-162
Identity = 282/286 (98.60%), Postives = 283/286 (98.95%), Query Frame = 0


Query: 1   MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA 60
           MFGRRSTSHHEEE NPEQITSKVNELKAKLGP+VGRNAIYCSDACLKRYLVARNWNVDKA
Sbjct: 1   MFGRRSTSHHEEENNPEQITSKVNELKAKLGPLVGRNAIYCSDACLKRYLVARNWNVDKA 60

Query: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120
           KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE
Sbjct: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA
Sbjct: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240

Query: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE 287
           AQLEYVHEEFSTLMIQDDIKCAAFWEQ EKQHH VNGYSSAVVAPE
Sbjct: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQDEKQHHTVNGYSSAVVAPE 286

[Close]

BLAST of CSPI03G42650 vs. NCBI nr
Match: XP_038906163.1 (CRAL-TRIO domain-containing protein C23B6.04c [Benincasa hispida] >XP_038906164.1 CRAL-TRIO domain-containing protein C23B6.04c [Benincasa hispida])

HSP 1 Score: 569.7 bits (1467), Expect = 1.4e-158
Identity = 274/286 (95.80%), Postives = 280/286 (97.90%), Query Frame = 0


Query: 1   MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA 60
           MFGR STSHHEEE NPEQITSKVNELKAKLG +VGRNAIYCSDACLKRYL ARNWNVDKA
Sbjct: 1   MFGRWSTSHHEEENNPEQITSKVNELKAKLGSLVGRNAIYCSDACLKRYLAARNWNVDKA 60

Query: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120
           +KMLEETFKWRS+YKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE
Sbjct: 61  RKMLEETFKWRSMYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA
Sbjct: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRF+YPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFIYPKKQESVELMKSYFDEENLPSEFGGK 240

Query: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE 287
           AQLEYVHEE+S +MIQDDIKCAAFWE GEKQHHIVNGYSSAVVAPE
Sbjct: 241 AQLEYVHEEYSRIMIQDDIKCAAFWEHGEKQHHIVNGYSSAVVAPE 286

[Close]

BLAST of CSPI03G42650 vs. TAIR 10
Match: AT1G75170.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 415.6 bits (1067), Expect = 3.2e-116
Identity = 197/286 (68.88%), Postives = 233/286 (81.47%), Query Frame = 0


Query: 4   RRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKAKKM 63
           + S+   +E+K      +K+ ELK  +G + GRN++YCSDACLKRYL ARNWNV KAKKM
Sbjct: 5   KNSSQTEQEQKEAALREAKMKELKTLIGQLSGRNSLYCSDACLKRYLEARNWNVGKAKKM 64

Query: 64  LEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLENQI 123
           LEET KWRS +KPEEIRW E++ E ETGK+Y+A FHDR GRTVLI++PG QNT SLENQ+
Sbjct: 65  LEETLKWRSSFKPEEIRWNEVSGEGETGKVYKAGFHDRHGRTVLILRPGLQNTKSLENQM 124

Query: 124 RHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLALAF 183
           +HLVYL+ENA+LNLPE QEQMSWLIDF GWS+STSVPIKSARET+NILQNHYPERLA+AF
Sbjct: 125 KHLVYLIENAILNLPEDQEQMSWLIDFTGWSMSTSVPIKSARETINILQNHYPERLAVAF 184

Query: 184 LYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQL 243
           LYNPPR+FEAFWK+VKY +D KTF KV+FVYPK  ESVELM ++FDEENLP+EFGGKA L
Sbjct: 185 LYNPPRLFEAFWKIVKYFIDAKTFVKVKFVYPKNSESVELMSTFFDEENLPTEFGGKALL 244

Query: 244 EYVHEEFSTLMIQDDIKCAAFWEQG---EKQHHIVNGYSSAVVAPE 287
           +Y +EEFS  M QDD+K A FW  G     Q H  NG+S A +APE
Sbjct: 245 QYNYEEFSKQMNQDDVKTANFWGLGHSNNNQLHASNGFSGAEIAPE 290

[Close]

BLAST of CSPI03G42650 vs. TAIR 10
Match: AT1G75170.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 415.6 bits (1067), Expect = 3.2e-116
Identity = 197/286 (68.88%), Postives = 233/286 (81.47%), Query Frame = 0


Query: 4   RRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKAKKM 63
           + S+   +E+K      +K+ ELK  +G + GRN++YCSDACLKRYL ARNWNV KAKKM
Sbjct: 5   KNSSQTEQEQKEAALREAKMKELKTLIGQLSGRNSLYCSDACLKRYLEARNWNVGKAKKM 64

Query: 64  LEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLENQI 123
           LEET KWRS +KPEEIRW E++ E ETGK+Y+A FHDR GRTVLI++PG QNT SLENQ+
Sbjct: 65  LEETLKWRSSFKPEEIRWNEVSGEGETGKVYKAGFHDRHGRTVLILRPGLQNTKSLENQM 124

Query: 124 RHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLALAF 183
           +HLVYL+ENA+LNLPE QEQMSWLIDF GWS+STSVPIKSARET+NILQNHYPERLA+AF
Sbjct: 125 KHLVYLIENAILNLPEDQEQMSWLIDFTGWSMSTSVPIKSARETINILQNHYPERLAVAF 184

Query: 184 LYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQL 243
           LYNPPR+FEAFWK+VKY +D KTF KV+FVYPK  ESVELM ++FDEENLP+EFGGKA L
Sbjct: 185 LYNPPRLFEAFWKIVKYFIDAKTFVKVKFVYPKNSESVELMSTFFDEENLPTEFGGKALL 244

Query: 244 EYVHEEFSTLMIQDDIKCAAFWEQG---EKQHHIVNGYSSAVVAPE 287
           +Y +EEFS  M QDD+K A FW  G     Q H  NG+S A +APE
Sbjct: 245 QYNYEEFSKQMNQDDVKTANFWGLGHSNNNQLHASNGFSGAEIAPE 290

[Close]

BLAST of CSPI03G42650 vs. TAIR 10
Match: AT4G36640.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 361.3 bits (926), Expect = 7.2e-100
Identity = 166/286 (58.04%), Postives = 221/286 (77.27%), Query Frame = 0


Query: 1   MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA 60
           MF RR+   H+ + +  Q  +KV ELK+ +GP+ G + ++CSDA L+R+L ARNW+V+KA
Sbjct: 1   MFRRRNA--HQLDNDDSQQDNKVRELKSAIGPLSGHSLVFCSDASLRRFLDARNWDVEKA 60

Query: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120
           KKM++ET KWRS YKP+EIRW ++A E ETGK  RASFHDR+GR VLIM+P  QN+TS E
Sbjct: 61  KKMIQETLKWRSTYKPQEIRWNQVAHEGETGKASRASFHDRQGRVVLIMRPAMQNSTSQE 120

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180
             IRHLVYL+ENA++NLP+GQ+QMSWLIDF GWS++ + P+K+ RE ++ILQN+YPERL 
Sbjct: 121 GNIRHLVYLLENAIINLPKGQKQMSWLIDFTGWSMAVNPPMKTTREIIHILQNYYPERLG 180

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           +AFLYNPPR+F+A ++  KY LDP+T +KV+FVYPK + S ELM ++FD ENLP EFGG+
Sbjct: 181 IAFLYNPPRLFQAVYRAAKYFLDPRTAEKVKFVYPKDKASDELMTTHFDVENLPKEFGGE 240

Query: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE 287
           A LEY HE+FS  M +DD+K A +W    K +   NG+S + V PE
Sbjct: 241 ATLEYDHEDFSRQMYEDDLKTAKYWGLEGKHYPKTNGFSPSDVVPE 284

[Close]

BLAST of CSPI03G42650 vs. TAIR 10
Match: AT1G75170.3 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 314.3 bits (804), Expect = 1.0e-85
Identity = 148/206 (71.84%), Postives = 173/206 (83.98%), Query Frame = 0


Query: 84  IAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLENQIRHLVYLMENALLNLPEGQEQ 143
           ++ E ETGK+Y+A FHDR GRTVLI++PG QNT SLENQ++HLVYL+ENA+LNLPE QEQ
Sbjct: 2   VSGEGETGKVYKAGFHDRHGRTVLILRPGLQNTKSLENQMKHLVYLIENAILNLPEDQEQ 61

Query: 144 MSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLALAFLYNPPRIFEAFWKVVKYLLD 203
           MSWLIDF GWS+STSVPIKSARET+NILQNHYPERLA+AFLYNPPR+FEAFWK+VKY +D
Sbjct: 62  MSWLIDFTGWSMSTSVPIKSARETINILQNHYPERLAVAFLYNPPRLFEAFWKIVKYFID 121

Query: 204 PKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQLEYVHEEFSTLMIQDDIKCAA 263
            KTF KV+FVYPK  ESVELM ++FDEENLP+EFGGKA L+Y +EEFS  M QDD+K A 
Sbjct: 122 AKTFVKVKFVYPKNSESVELMSTFFDEENLPTEFGGKALLQYNYEEFSKQMNQDDVKTAN 181

Query: 264 FWEQG---EKQHHIVNGYSSAVVAPE 287
           FW  G     Q H  NG+S A +APE
Sbjct: 182 FWGLGHSNNNQLHASNGFSGAEIAPE 207

[Close]

BLAST of CSPI03G42650 vs. TAIR 10
Match: AT4G36640.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 301.6 bits (771), Expect = 6.7e-82
Identity = 136/224 (60.71%), Postives = 176/224 (78.57%), Query Frame = 0


Query: 63  MLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLENQ 122
           M++ET KWRS YKP+EIRW ++A E ETGK  RASFHDR+GR VLIM+P  QN+TS E  
Sbjct: 1   MIQETLKWRSTYKPQEIRWNQVAHEGETGKASRASFHDRQGRVVLIMRPAMQNSTSQEGN 60

Query: 123 IRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLALA 182
           IRHLVYL+ENA++NLP+GQ+QMSWLIDF GWS++ + P+K+ RE ++ILQN+YPERL +A
Sbjct: 61  IRHLVYLLENAIINLPKGQKQMSWLIDFTGWSMAVNPPMKTTREIIHILQNYYPERLGIA 120

Query: 183 FLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQ 242
           FLYNPPR+F+A ++  KY LDP+T +KV+FVYPK + S ELM ++FD ENLP EFGG+A 
Sbjct: 121 FLYNPPRLFQAVYRAAKYFLDPRTAEKVKFVYPKDKASDELMTTHFDVENLPKEFGGEAT 180

Query: 243 LEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE 287
           LEY HE+FS  M +DD+K A +W    K +   NG+S + V PE
Sbjct: 181 LEYDHEDFSRQMYEDDLKTAKYWGLEGKHYPKTNGFSPSDVVPE 224

The following BLAST results are available for this feature:

BLAST of CSPI03G42650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9UU99	1.7e-29	31.50	CRAL-TRIO domain-containing protein C23B6.04c OS=Schizosaccharomyces pombe (stra...	[more]
P53844	3.9e-26	32.09	Phosphatidylinositol transfer protein PDR17 OS=Saccharomyces cerevisiae (strain ...	[more]
Q55CU8	5.7e-25	28.94	Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=...	[more]
P53860	2.8e-24	28.57	Phosphatidylinositol transfer protein PDR16 OS=Saccharomyces cerevisiae (strain ...	[more]
P53989	4.3e-17	32.13	SEC14 cytosolic factor OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 376...	[more]

BLAST of CSPI03G42650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CRQ6	1.8e-162	98.60	CRAL-TRIO domain-containing protein C23B6.04c OS=Cucumis melo OX=3656 GN=LOC1035...	[more]
A0A5D3D813	1.8e-162	98.60	CRAL-TRIO domain-containing protein C23B6.04c OS=Cucumis melo var. makuwa OX=119...	[more]
A0A6J1J0L2	1.1e-156	94.41	CRAL-TRIO domain-containing protein C23B6.04c isoform X1 OS=Cucurbita maxima OX=...	[more]
A0A6J1FJV0	1.4e-156	94.06	CRAL-TRIO domain-containing protein C23B6.04c isoform X1 OS=Cucurbita moschata O...	[more]
A0A6J1IUY5	1.6e-147	95.47	CRAL-TRIO domain-containing protein C23B6.04c isoform X2 OS=Cucurbita maxima OX=...	[more]

BLAST of CSPI03G42650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031738483.1	2.3e-164	100.00	CRAL-TRIO domain-containing protein C23B6.04c isoform X1 [Cucumis sativus]	[more]
XP_031738484.1	2.3e-164	100.00	CRAL-TRIO domain-containing protein C23B6.04c isoform X2 [Cucumis sativus]	[more]
XP_004147786.1	2.3e-164	100.00	CRAL-TRIO domain-containing protein C23B6.04c isoform X3 [Cucumis sativus] >XP_0...	[more]
XP_008466570.1	3.6e-162	98.60	PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c [Cucumis melo] >XP_0084...	[more]
XP_038906163.1	1.4e-158	95.80	CRAL-TRIO domain-containing protein C23B6.04c [Benincasa hispida] >XP_038906164....	[more]

BLAST of CSPI03G42650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G75170.1	3.2e-116	68.88	Sec14p-like phosphatidylinositol transfer family protein	[more]
AT1G75170.2	3.2e-116	68.88	Sec14p-like phosphatidylinositol transfer family protein	[more]
AT4G36640.1	7.2e-100	58.04	Sec14p-like phosphatidylinositol transfer family protein	[more]
AT1G75170.3	1.0e-85	71.84	Sec14p-like phosphatidylinositol transfer family protein	[more]
AT4G36640.2	6.7e-82	60.71	Sec14p-like phosphatidylinositol transfer family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011074	CRAL/TRIO, N-terminal domain	SMART	SM01100	CRAL_TRIO_N_2	coord: 42..67 e-value: 2.3E-8 score: 43.8
IPR011074	CRAL/TRIO, N-terminal domain	PFAM	PF03765	CRAL_TRIO_N	coord: 26..66 e-value: 4.1E-8 score: 33.3
IPR001251	CRAL-TRIO lipid binding domain	SMART	SM00516	sec14_4	coord: 87..242 e-value: 1.7E-52 score: 190.4
IPR001251	CRAL-TRIO lipid binding domain	PFAM	PF00650	CRAL_TRIO	coord: 89..240 e-value: 1.6E-37 score: 128.7
IPR001251	CRAL-TRIO lipid binding domain	PROSITE	PS50191	CRAL_TRIO	coord: 81..245 score: 20.80994
IPR001251	CRAL-TRIO lipid binding domain	CDD	cd00170	SEC14	coord: 99..240 e-value: 1.8945E-44 score: 146.376
IPR036865	CRAL-TRIO lipid binding domain superfamily	GENE3D	3.40.525.10		coord: 4..276 e-value: 2.6E-80 score: 271.9
IPR036865	CRAL-TRIO lipid binding domain superfamily	SUPERFAMILY	52087	CRAL/TRIO domain	coord: 81..255
None	No IPR available	PANTHER	PTHR45824	GH16843P	coord: 6..285
None	No IPR available	PANTHER	PTHR45824:SF17	DIVERGENT CRAL/TRIO DOMAIN PROTEIN	coord: 6..285
IPR036273	CRAL/TRIO, N-terminal domain superfamily	SUPERFAMILY	46938	CRAL/TRIO N-terminal domain	coord: 11..83

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G42650.1	CSPI03G42650.1	mRNA
CSPI03G42650.2	CSPI03G42650.2	mRNA
CSPI03G42650.3	CSPI03G42650.3	mRNA
CSPI03G42650.4	CSPI03G42650.4	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0120009	intermembrane lipid transfer
biological_process	GO:0006869	lipid transport
biological_process	GO:0015914	phospholipid transport
cellular_component	GO:0016020	membrane
molecular_function	GO:0008526	phosphatidylinositol transfer activity