CSPI03G42430 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G42430
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Calmodulin
Location	Chr3: 36465608 .. 36466252 (-)
RNA-Seq Expression	CSPI03G42430
Synteny	CSPI03G42430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACCACCAAATTTTCCTAAATGGCCATTTCATCCATTGCCGTAATTCCCTTCAAATGGTTTACCAAAACCAATTTCAAGCTCAACTTCCCTCTCCTTCACTCCAAACACAAACCAACTTCCTCTAAACCTTCTACGTCGGGGCCAGGGCCGCGGCCACTTGCCGAGGTCGCTTTTCGCCATCTCGACGTTGATGGAGATGGAAAAATCTCTTGCAACGAGCTACAATCCTACTTCGCATCGATAGGGGAGTACATGACATGGAATGATGCTCGGAGTGTGATTGGAGATTTTGATAGAGACGGTGACGAGTTACTAGAGCTTGGAGATTTTGAGCGAATGGTAAAGGGAGAGGAGGAGGAAGAGGAGGATTTGAAAAGGGCTTTTGAGATGTTTGAAGGTGAGAAGGGATGTGGATTCATTGAGGCAACGGGATTGCAAAAGATGTTTGGGAGGCTTGGATACGTTAAATCAAAAGAAGAGTGCATGGCTATGATCAAAGTGTTTGATGTTGATGGCGATGGTGTAATTGATTATCATGAATTTATGAGAATGATGACTTGACTATCTAATTTAACCTTCGAATAAGTTGTCCTTTCATTTTCCCTTTTGTTTTTGGGTGAATATATGTTAATGAAAACTGGGA

mRNA sequence

AAACCACCAAATTTTCCTAAATGGCCATTTCATCCATTGCCGTAATTCCCTTCAAATGGTTTACCAAAACCAATTTCAAGCTCAACTTCCCTCTCCTTCACTCCAAACACAAACCAACTTCCTCTAAACCTTCTACGTCGGGGCCAGGGCCGCGGCCACTTGCCGAGGTCGCTTTTCGCCATCTCGACGTTGATGGAGATGGAAAAATCTCTTGCAACGAGCTACAATCCTACTTCGCATCGATAGGGGAGTACATGACATGGAATGATGCTCGGAGTGTGATTGGAGATTTTGATAGAGACGGTGACGAGTTACTAGAGCTTGGAGATTTTGAGCGAATGGTAAAGGGAGAGGAGGAGGAAGAGGAGGATTTGAAAAGGGCTTTTGAGATGTTTGAAGGTGAGAAGGGATGTGGATTCATTGAGGCAACGGGATTGCAAAAGATGTTTGGGAGGCTTGGATACGTTAAATCAAAAGAAGAGTGCATGGCTATGATCAAAGTGTTTGATGTTGATGGCGATGGTGTAATTGATTATCATGAATTTATGAGAATGATGACTTGACTATCTAATTTAACCTTCGAATAAGTTGTCCTTTCATTTTCCCTTTTGTTTTTGGGTGAATATATGTTAATGAAAACTGGGA

Coding sequence (CDS)

ATGGCCATTTCATCCATTGCCGTAATTCCCTTCAAATGGTTTACCAAAACCAATTTCAAGCTCAACTTCCCTCTCCTTCACTCCAAACACAAACCAACTTCCTCTAAACCTTCTACGTCGGGGCCAGGGCCGCGGCCACTTGCCGAGGTCGCTTTTCGCCATCTCGACGTTGATGGAGATGGAAAAATCTCTTGCAACGAGCTACAATCCTACTTCGCATCGATAGGGGAGTACATGACATGGAATGATGCTCGGAGTGTGATTGGAGATTTTGATAGAGACGGTGACGAGTTACTAGAGCTTGGAGATTTTGAGCGAATGGTAAAGGGAGAGGAGGAGGAAGAGGAGGATTTGAAAAGGGCTTTTGAGATGTTTGAAGGTGAGAAGGGATGTGGATTCATTGAGGCAACGGGATTGCAAAAGATGTTTGGGAGGCTTGGATACGTTAAATCAAAAGAAGAGTGCATGGCTATGATCAAAGTGTTTGATGTTGATGGCGATGGTGTAATTGATTATCATGAATTTATGAGAATGATGACTTGA

Protein sequence

MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTSGPGPRPLAEVAFRHLDVDGDGKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKGEEEEEEDLKRAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGVIDYHEFMRMMT*

Homology

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BLAST of CSPI03G42430 vs. ExPASy Swiss-Prot
Match: Q8L3R2 (Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 PE=2 SV=2)

HSP 1 Score: 134.4 bits (337), Expect = 1.3e-30
Identity = 77/192 (40.10%), Postives = 103/192 (53.65%), Query Frame = 0


Query: 11  FKWFTKTNFKLNFPLLHSKHKPTSSKPST---------------SGPGPRPLAEVAFRHL 70
           FKWF+    KLN    + +  P S+  ST               S    +      F H 
Sbjct: 13  FKWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEELRQVFSHF 72

Query: 71  DVDGDGKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMV------- 130
           D DGDGKIS  EL+ YF S+GEY++   A+  I + D D D  L   DF  ++       
Sbjct: 73  DSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMTRRDLYG 132

Query: 131 KGEEEEEEDLKRAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDG 181
            GE + + +LK AFEMFE EKG G I   GLQKM  +LG  ++  EC AMIK +D+DG+G
Sbjct: 133 DGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYDIDGNG 192

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BLAST of CSPI03G42430 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.6e-17
Identity = 50/134 (37.31%), Postives = 83/134 (61.94%), Query Frame = 0


Query: 52  FRHLDVDGDGKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDF----ERM 111
           F+  D +GDGKI+ NEL+ +F S+G  +  N+   +I   D +GD  +++ +F    + M
Sbjct: 10  FQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSLYQEM 69

Query: 112 VKGEEEEEEDLKRAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSK--EECMAMIKVFDVD 171
           V+ E+EEEED++ AF +F+ + G GFI    L+ +   +G  + +  E+C  MI   DVD
Sbjct: 70  VE-EKEEEEDMREAFRVFD-QNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDVD 129

Query: 172 GDGVIDYHEFMRMM 180
           GDG++++ EF +MM
Sbjct: 130 GDGMVNFKEFKQMM 141

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BLAST of CSPI03G42430 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 4.7e-17
Identity = 49/144 (34.03%), Postives = 84/144 (58.33%), Query Frame = 0


Query: 38  STSGPGPRPLAEVAFRHLDVDGDGKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDE 97
           S++G       E  F ++D + DG+IS  ELQ  F ++GE ++  +A + +   D DGD 
Sbjct: 34  SSNGEDKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDG 93

Query: 98  LLELGDFERMVKGEEEEEE--DLKRAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEEC 157
           +L+  +F +++K ++EEE+  +LK AF ++  E G   I    L+ M  +LG  ++ ++C
Sbjct: 94  MLDFEEFSQLIKVDDEEEKKMELKGAFRLYIAE-GEDCITPRSLKMMLKKLGESRTTDDC 153

Query: 158 MAMIKVFDVDGDGVIDYHEFMRMM 180
             MI  FD++ DGV+ + EF  MM
Sbjct: 154 RVMISAFDLNADGVLSFDEFALMM 176

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BLAST of CSPI03G42430 vs. ExPASy Swiss-Prot
Match: O22845 (Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2)

HSP 1 Score: 88.2 bits (217), Expect = 1.0e-16
Identity = 61/183 (33.33%), Postives = 91/183 (49.73%), Query Frame = 0


Query: 8   VIPFKWFTKTNFKLNFPLLHSK-HKPTSSKPSTSGPGPRPLAEVAFRHLDVDGDGKISCN 67
           + P  WF KT  K + P   +    P+SS   T    P  L  V F+  D +GDG+I+  
Sbjct: 26  LFPLSWFDKTLHKNSPPSPSTMLPSPSSSSAPTKRIDPSELKRV-FQMFDKNGDGRITKE 85

Query: 68  ELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKG--------EEEEEEDL 127
           EL     ++G Y+   D   +I   D +GD  +++ +FE +            E EEED+
Sbjct: 86  ELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFESLYSSIVDEHHNDGETEEEDM 145

Query: 128 KRAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSK--EECMAMIKVFDVDGDGVIDYHEFM 180
           K AF +F+ + G GFI    L+ +   LG  + K  + C  MI   D DGDG ++Y EF+
Sbjct: 146 KDAFNVFD-QDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKEFL 205

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BLAST of CSPI03G42430 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.4e-16
Identity = 56/135 (41.48%), Postives = 79/135 (58.52%), Query Frame = 0


Query: 52  FRHLDVDGDGKISCNELQSYF-ASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKG 111
           F   D DGDG+IS  EL+     ++GE ++  +A  ++   D DGD LL   +F R+V+ 
Sbjct: 16  FATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVRLVQA 75

Query: 112 EEEEEED------LKRAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDV 171
            E EEED      L+ AF M+E E G G I  T L++M  RLG  +  ++C AMI  FD+
Sbjct: 76  AEVEEEDERRGTGLREAFGMYEME-GEGCITPTSLRRMLRRLGSDQDIDDCRAMICRFDL 135

Query: 172 DGDGVIDYHEFMRMM 180
           +GDGV+ + EF  MM
Sbjct: 136 NGDGVLSFDEFKIMM 149

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BLAST of CSPI03G42430 vs. ExPASy TrEMBL
Match: A0A0A0LG17 (Calmodulin OS=Cucumis sativus OX=3659 GN=Csa_3G854250 PE=4 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.1e-98
Identity = 179/180 (99.44%), Postives = 180/180 (100.00%), Query Frame = 0


Query: 1   MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTSGPGPRPLAEVAFRHLDVDGD 60
           MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTSGPGPRPLAEVAFRHLDVDGD
Sbjct: 1   MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTSGPGPRPLAEVAFRHLDVDGD 60

Query: 61  GKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKGEEEEEEDLKR 120
           GKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKGEEEEEEDLKR
Sbjct: 61  GKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKGEEEEEEDLKR 120

Query: 121 AFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGVIDYHEFMRMMT 180
           AFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGVIDYHEF+RMMT
Sbjct: 121 AFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGVIDYHEFLRMMT 180

[Close]

BLAST of CSPI03G42430 vs. ExPASy TrEMBL
Match: A0A5A7UHX6 (Putative calcium-binding protein CML41 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27333G00010 PE=4 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 5.6e-82
Identity = 158/181 (87.29%), Postives = 168/181 (92.82%), Query Frame = 0


Query: 1   MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTSGPGPRPLAEVAFRHLDVDGD 60
           MAIS+IA IPFKWFTKTNFKLNFPLLHSKHKP SSKP    P P PLAEVAFRHLD+DGD
Sbjct: 1   MAISTIA-IPFKWFTKTNFKLNFPLLHSKHKPISSKP----PTPPPLAEVAFRHLDIDGD 60

Query: 61  GKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKGEEEE-EEDLK 120
           GKIS +EL+SYFASIGEYMTW+ A+SVIGDFD+DGDELLELGDFER+VKGEEEE EEDLK
Sbjct: 61  GKISGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKGEEEEQEEDLK 120

Query: 121 RAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGVIDYHEFMRMM 180
           RAFEMFEGEKGCGFI ATGLQKMFGRLGYVKSKE+CM MIKVFDVDGDGVIDYHEF+RMM
Sbjct: 121 RAFEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGVIDYHEFLRMM 176

[Close]

BLAST of CSPI03G42430 vs. ExPASy TrEMBL
Match: A0A1S3CT00 (probable calcium-binding protein CML41 OS=Cucumis melo OX=3656 GN=LOC103503982 PE=4 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 5.6e-82
Identity = 158/181 (87.29%), Postives = 168/181 (92.82%), Query Frame = 0


Query: 1   MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTSGPGPRPLAEVAFRHLDVDGD 60
           MAIS+IA IPFKWFTKTNFKLNFPLLHSKHKP SSKP    P P PLAEVAFRHLD+DGD
Sbjct: 1   MAISTIA-IPFKWFTKTNFKLNFPLLHSKHKPISSKP----PTPPPLAEVAFRHLDIDGD 60

Query: 61  GKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKGEEEE-EEDLK 120
           GKIS +EL+SYFASIGEYMTW+ A+SVIGDFD+DGDELLELGDFER+VKGEEEE EEDLK
Sbjct: 61  GKISGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKGEEEEQEEDLK 120

Query: 121 RAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGVIDYHEFMRMM 180
           RAFEMFEGEKGCGFI ATGLQKMFGRLGYVKSKE+CM MIKVFDVDGDGVIDYHEF+RMM
Sbjct: 121 RAFEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGVIDYHEFLRMM 176

[Close]

BLAST of CSPI03G42430 vs. ExPASy TrEMBL
Match: A0A6J1IIY2 (probable calcium-binding protein CML41 OS=Cucurbita maxima OX=3661 GN=LOC111473738 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 5.1e-67
Identity = 140/186 (75.27%), Postives = 151/186 (81.18%), Query Frame = 0


Query: 1   MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTS-----GPGPRPLAEVAFRHL 60
           MA+S I+  PFKWF+ TNFKLNFP L  KHKPT S P         P P PLAE AFRHL
Sbjct: 15  MALSPIST-PFKWFSNTNFKLNFPRLRFKHKPTPSNPPPPPLLLLTPAP-PLAEAAFRHL 74

Query: 61  DVDGDGKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVK-GEEEE 120
           D DGDGKIS +ELQSYFAS+GEYMT +DARS+I +FD DGD LLELGDFER+VK GEEEE
Sbjct: 75  DADGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEEEE 134

Query: 121 EEDLKRAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGVIDYHE 180
           +EDLK AFEMFEGEKGCGFI   GLQKMFGRLGYVKS EECMAMI VFDVDGDGVIDY E
Sbjct: 135 KEDLKMAFEMFEGEKGCGFIGPPGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYRE 194

[Close]

BLAST of CSPI03G42430 vs. ExPASy TrEMBL
Match: A0A6J1F7Y7 (probable calcium-binding protein CML41 OS=Cucurbita moschata OX=3662 GN=LOC111442970 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 1.3e-65
Identity = 137/185 (74.05%), Postives = 150/185 (81.08%), Query Frame = 0


Query: 1   MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTS----GPGPRPLAEVAFRHLD 60
           MA+S I+  PFKWF+ TN KLN P L  KHKPT S P        P P PLAE AFRHLD
Sbjct: 1   MALSPIST-PFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLTPAP-PLAEAAFRHLD 60

Query: 61  VDGDGKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVK-GEEEEE 120
            DGDGKIS +ELQSYFAS+GEYMT +DARS+I +FD DGD LLELGDFER+VK GE+EE+
Sbjct: 61  ADGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEQEEK 120

Query: 121 EDLKRAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGVIDYHEF 180
           EDLKRAFEMFEGEKGCGFI   GLQKMFGRLGYVKS EECMAMI VFDVDG+GVIDY EF
Sbjct: 121 EDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGNGVIDYREF 180

[Close]

BLAST of CSPI03G42430 vs. NCBI nr
Match: XP_004147818.1 (probable calcium-binding protein CML41 [Cucumis sativus])

HSP 1 Score: 369.0 bits (946), Expect = 2.3e-98
Identity = 179/180 (99.44%), Postives = 180/180 (100.00%), Query Frame = 0


Query: 1   MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTSGPGPRPLAEVAFRHLDVDGD 60
           MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTSGPGPRPLAEVAFRHLDVDGD
Sbjct: 1   MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTSGPGPRPLAEVAFRHLDVDGD 60

Query: 61  GKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKGEEEEEEDLKR 120
           GKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKGEEEEEEDLKR
Sbjct: 61  GKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKGEEEEEEDLKR 120

Query: 121 AFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGVIDYHEFMRMMT 180
           AFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGVIDYHEF+RMMT
Sbjct: 121 AFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGVIDYHEFLRMMT 180

[Close]

BLAST of CSPI03G42430 vs. NCBI nr
Match: KAE8651319.1 (hypothetical protein Csa_001024 [Cucumis sativus])

HSP 1 Score: 345.1 bits (884), Expect = 3.6e-91
Identity = 168/170 (98.82%), Postives = 169/170 (99.41%), Query Frame = 0


Query: 1   MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTSGPGPRPLAEVAFRHLDVDGD 60
           MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTSGPGPRPLAEVAFRHLDVDGD
Sbjct: 1   MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTSGPGPRPLAEVAFRHLDVDGD 60

Query: 61  GKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKGEEEEEEDLKR 120
           GKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKGEEEEEEDLKR
Sbjct: 61  GKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKGEEEEEEDLKR 120

Query: 121 AFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGVI 171
           AFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDG +
Sbjct: 121 AFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGEV 170

[Close]

BLAST of CSPI03G42430 vs. NCBI nr
Match: XP_008466624.1 (PREDICTED: probable calcium-binding protein CML41 [Cucumis melo] >KAA0055432.1 putative calcium-binding protein CML41 [Cucumis melo var. makuwa])

HSP 1 Score: 313.5 bits (802), Expect = 1.2e-81
Identity = 158/181 (87.29%), Postives = 168/181 (92.82%), Query Frame = 0


Query: 1   MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTSGPGPRPLAEVAFRHLDVDGD 60
           MAIS+IA IPFKWFTKTNFKLNFPLLHSKHKP SSKP    P P PLAEVAFRHLD+DGD
Sbjct: 1   MAISTIA-IPFKWFTKTNFKLNFPLLHSKHKPISSKP----PTPPPLAEVAFRHLDIDGD 60

Query: 61  GKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKGEEEE-EEDLK 120
           GKIS +EL+SYFASIGEYMTW+ A+SVIGDFD+DGDELLELGDFER+VKGEEEE EEDLK
Sbjct: 61  GKISGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKGEEEEQEEDLK 120

Query: 121 RAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGVIDYHEFMRMM 180
           RAFEMFEGEKGCGFI ATGLQKMFGRLGYVKSKE+CM MIKVFDVDGDGVIDYHEF+RMM
Sbjct: 121 RAFEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGVIDYHEFLRMM 176

[Close]

BLAST of CSPI03G42430 vs. NCBI nr
Match: XP_038905487.1 (probable calcium-binding protein CML41 [Benincasa hispida])

HSP 1 Score: 274.2 bits (700), Expect = 7.8e-70
Identity = 144/180 (80.00%), Postives = 156/180 (86.67%), Query Frame = 0


Query: 1   MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTSGPGPRPLAEVAFRHLDVDGD 60
           MAIS+I+ IP KWF+    KLNFP LHSKHKPTSSKP +      PLAEVAFRHLDVDGD
Sbjct: 1   MAISTIS-IPLKWFST---KLNFPRLHSKHKPTSSKPPS-----LPLAEVAFRHLDVDGD 60

Query: 61  GKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKG-EEEEEEDLK 120
           GKIS +EL++YFASIGEYMT + A+SVIGDFD DGDELLELGDF+R+VKG EEEEEEDLK
Sbjct: 61  GKISSDELRTYFASIGEYMTRDGAQSVIGDFDEDGDELLELGDFQRLVKGEEEEEEEDLK 120

Query: 121 RAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGVIDYHEFMRMM 180
           RAFEMFEGEKGCGFI   GLQKMFGRLGYVKSKEEC AMIKVFDVDGDGVIDY EF+RMM
Sbjct: 121 RAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECSAMIKVFDVDGDGVIDYREFLRMM 171

[Close]

BLAST of CSPI03G42430 vs. NCBI nr
Match: XP_023535419.1 (calmodulin-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 263.8 bits (673), Expect = 1.1e-66
Identity = 139/186 (74.73%), Postives = 151/186 (81.18%), Query Frame = 0


Query: 1   MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTS-----GPGPRPLAEVAFRHL 60
           MA+S ++  PFKWF+ TNF LNFP L  KHKPT S P         P P PLAE AFRHL
Sbjct: 38  MALSPVST-PFKWFSNTNFNLNFPRLRFKHKPTRSNPPPPPLLLLTPAP-PLAEAAFRHL 97

Query: 61  DVDGDGKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVK-GEEEE 120
           D DGDGKIS +ELQSYFAS+GEYMT +DARS+I +FD DGD LLELGDFER+VK GEEEE
Sbjct: 98  DADGDGKISGDELQSYFASVGEYMTRDDARSLIREFDWDGDLLLELGDFERLVKGGEEEE 157

Query: 121 EEDLKRAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGVIDYHE 180
           +EDLKRAFEMFEGEKGCGFI   GLQKMFGRLGYVKS EECMAMI VFDVDGDGVIDY E
Sbjct: 158 KEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYRE 217

[Close]

BLAST of CSPI03G42430 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 134.4 bits (337), Expect = 9.0e-32
Identity = 77/192 (40.10%), Postives = 103/192 (53.65%), Query Frame = 0


Query: 11  FKWFTKTNFKLNFPLLHSKHKPTSSKPST---------------SGPGPRPLAEVAFRHL 70
           FKWF+    KLN    + +  P S+  ST               S    +      F H 
Sbjct: 13  FKWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEELRQVFSHF 72

Query: 71  DVDGDGKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMV------- 130
           D DGDGKIS  EL+ YF S+GEY++   A+  I + D D D  L   DF  ++       
Sbjct: 73  DSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMTRRDLYG 132

Query: 131 KGEEEEEEDLKRAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDG 181
            GE + + +LK AFEMFE EKG G I   GLQKM  +LG  ++  EC AMIK +D+DG+G
Sbjct: 133 DGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYDIDGNG 192

[Close]

BLAST of CSPI03G42430 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 90.9 bits (224), Expect = 1.1e-18
Identity = 50/134 (37.31%), Postives = 83/134 (61.94%), Query Frame = 0


Query: 52  FRHLDVDGDGKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDF----ERM 111
           F+  D +GDGKI+ NEL+ +F S+G  +  N+   +I   D +GD  +++ +F    + M
Sbjct: 10  FQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSLYQEM 69

Query: 112 VKGEEEEEEDLKRAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSK--EECMAMIKVFDVD 171
           V+ E+EEEED++ AF +F+ + G GFI    L+ +   +G  + +  E+C  MI   DVD
Sbjct: 70  VE-EKEEEEDMREAFRVFD-QNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDVD 129

Query: 172 GDGVIDYHEFMRMM 180
           GDG++++ EF +MM
Sbjct: 130 GDGMVNFKEFKQMM 141

[Close]

BLAST of CSPI03G42430 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 89.4 bits (220), Expect = 3.3e-18
Identity = 49/144 (34.03%), Postives = 84/144 (58.33%), Query Frame = 0


Query: 38  STSGPGPRPLAEVAFRHLDVDGDGKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDE 97
           S++G       E  F ++D + DG+IS  ELQ  F ++GE ++  +A + +   D DGD 
Sbjct: 34  SSNGEDKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDG 93

Query: 98  LLELGDFERMVKGEEEEEE--DLKRAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEEC 157
           +L+  +F +++K ++EEE+  +LK AF ++  E G   I    L+ M  +LG  ++ ++C
Sbjct: 94  MLDFEEFSQLIKVDDEEEKKMELKGAFRLYIAE-GEDCITPRSLKMMLKKLGESRTTDDC 153

Query: 158 MAMIKVFDVDGDGVIDYHEFMRMM 180
             MI  FD++ DGV+ + EF  MM
Sbjct: 154 RVMISAFDLNADGVLSFDEFALMM 176

[Close]

BLAST of CSPI03G42430 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 89.4 bits (220), Expect = 3.3e-18
Identity = 49/144 (34.03%), Postives = 84/144 (58.33%), Query Frame = 0


Query: 38  STSGPGPRPLAEVAFRHLDVDGDGKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDE 97
           S++G       E  F ++D + DG+IS  ELQ  F ++GE ++  +A + +   D DGD 
Sbjct: 34  SSNGEDKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDG 93

Query: 98  LLELGDFERMVKGEEEEEE--DLKRAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEEC 157
           +L+  +F +++K ++EEE+  +LK AF ++  E G   I    L+ M  +LG  ++ ++C
Sbjct: 94  MLDFEEFSQLIKVDDEEEKKMELKGAFRLYIAE-GEDCITPRSLKMMLKKLGESRTTDDC 153

Query: 158 MAMIKVFDVDGDGVIDYHEFMRMM 180
             MI  FD++ DGV+ + EF  MM
Sbjct: 154 RVMISAFDLNADGVLSFDEFALMM 176

[Close]

BLAST of CSPI03G42430 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 88.2 bits (217), Expect = 7.4e-18
Identity = 61/183 (33.33%), Postives = 91/183 (49.73%), Query Frame = 0


Query: 8   VIPFKWFTKTNFKLNFPLLHSK-HKPTSSKPSTSGPGPRPLAEVAFRHLDVDGDGKISCN 67
           + P  WF KT  K + P   +    P+SS   T    P  L  V F+  D +GDG+I+  
Sbjct: 26  LFPLSWFDKTLHKNSPPSPSTMLPSPSSSSAPTKRIDPSELKRV-FQMFDKNGDGRITKE 85

Query: 68  ELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKG--------EEEEEEDL 127
           EL     ++G Y+   D   +I   D +GD  +++ +FE +            E EEED+
Sbjct: 86  ELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFESLYSSIVDEHHNDGETEEEDM 145

Query: 128 KRAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSK--EECMAMIKVFDVDGDGVIDYHEFM 180
           K AF +F+ + G GFI    L+ +   LG  + K  + C  MI   D DGDG ++Y EF+
Sbjct: 146 KDAFNVFD-QDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKEFL 205

The following BLAST results are available for this feature:

BLAST of CSPI03G42430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L3R2	1.3e-30	40.10	Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 ...	[more]
Q9SU00	1.6e-17	37.31	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]
Q9SRE6	4.7e-17	34.03	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1	[more]
O22845	1.0e-16	33.33	Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2	[more]
Q8RYK0	1.4e-16	41.48	Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of CSPI03G42430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LG17	1.1e-98	99.44	Calmodulin OS=Cucumis sativus OX=3659 GN=Csa_3G854250 PE=4 SV=1	[more]
A0A5A7UHX6	5.6e-82	87.29	Putative calcium-binding protein CML41 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CT00	5.6e-82	87.29	probable calcium-binding protein CML41 OS=Cucumis melo OX=3656 GN=LOC103503982 P...	[more]
A0A6J1IIY2	5.1e-67	75.27	probable calcium-binding protein CML41 OS=Cucurbita maxima OX=3661 GN=LOC1114737...	[more]
A0A6J1F7Y7	1.3e-65	74.05	probable calcium-binding protein CML41 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]

BLAST of CSPI03G42430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147818.1	2.3e-98	99.44	probable calcium-binding protein CML41 [Cucumis sativus]	[more]
KAE8651319.1	3.6e-91	98.82	hypothetical protein Csa_001024 [Cucumis sativus]	[more]
XP_008466624.1	1.2e-81	87.29	PREDICTED: probable calcium-binding protein CML41 [Cucumis melo] >KAA0055432.1 p...	[more]
XP_038905487.1	7.8e-70	80.00	probable calcium-binding protein CML41 [Benincasa hispida]	[more]
XP_023535419.1	1.1e-66	74.73	calmodulin-like protein 2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI03G42430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G50770.1	9.0e-32	40.10	calmodulin-like 41	[more]
AT4G12860.1	1.1e-18	37.31	EF hand calcium-binding protein family	[more]
AT1G76650.1	3.3e-18	34.03	calmodulin-like 38	[more]
AT1G76650.3	3.3e-18	34.03	calmodulin-like 38	[more]
AT2G43290.1	7.4e-18	33.33	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 101..128
None	No IPR available	GENE3D	1.10.238.10		coord: 111..180 e-value: 2.1E-17 score: 64.8
None	No IPR available	GENE3D	1.10.238.10		coord: 25..110 e-value: 3.2E-13 score: 52.0
None	No IPR available	PANTHER	PTHR23050:SF393	CALMODULIN	coord: 23..180
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 23..180
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 83..111 e-value: 8.7 score: 10.2 coord: 47..75 e-value: 0.033 score: 23.3 coord: 154..180 e-value: 0.0087 score: 25.3
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 51..108 e-value: 3.2E-8 score: 33.9
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 154..180 e-value: 2.3E-8 score: 33.0
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 150..180 score: 15.545536
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 43..78 score: 12.225983
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 48..109 e-value: 1.60521E-9 score: 49.8537
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 117..180 e-value: 5.7771E-11 score: 53.7057
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 56..68
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 163..175
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 50..180

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G42430.1	CSPI03G42430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050790	regulation of catalytic activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030234	enzyme regulator activity