CSPI03G42320 (gene) Cucumber (PI 183967) v1

BLAST of CSPI03G42320 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 4.7e-134
Identity = 250/516 (48.45%), Postives = 341/516 (66.09%), Query Frame = 0

Query: 19  SLFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWPVIGHLHLLSSSEPGHKTL 78
           SL  L   L   F LL     F  ++  N ++  P   GAWP+IGHLHLL+  +  HK L
Sbjct: 3   SLHCLETLLLGFFVLLPCFFYFVWKKPNNKIKEPPQPAGAWPIIGHLHLLARGDLPHKIL 62

Query: 79  GKMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFASRPKLTSAKLLGYNNSMFG 138
              AD NGP+F ++LG+H+A+VV+N EIA+EC TTND  F +RP   +AK++GYN  M G
Sbjct: 63  SSFADKNGPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLG 122

Query: 139 LAQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINKLYQLCTKEK---PLVEMK 198
           +A YGP+WR MRK++ LE LS  RLQ  + +  SEI  S  +LY+L   +     LV+MK
Sbjct: 123 VAPYGPYWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQNIDFCLVDMK 182

Query: 199 AWFEDITLNIMFKIIFGKRFTDDLKG-------DQDHRKTFRDLMELFGVFVPSDSLPFL 258
            W  D+TLN+  K++ GKRF               +  KT R++  L G FV SD LP+L
Sbjct: 183 QWLADLTLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLPYL 242

Query: 259 SWLDLGGYEKAMKTTSKVLDEVFDKWLEEHRQRKIE-NNDNGAEDFMDVMLSIIKDDDEQ 318
            WLDLGG+EK MK T K LD +F  WL+EH+++++    ++  +DFMDVMLSI+   +E 
Sbjct: 243 RWLDLGGHEKEMKRTVKELDILFKGWLDEHKRKRLSGGKEDDDQDFMDVMLSIL---EES 302

Query: 319 LSGYVGDSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQVGRQKQV 378
             G   D++ K  CLA+IL G+DTT  T+TW LSLLLNN   LKKAQ EL+  VGR + V
Sbjct: 303 KLGNDVDTINKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLHVGRDRNV 362

Query: 379 TESDVKNLIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVNIQKLQRD 438
            ESD+  L Y+ AI+KE+LRLYP  P+  P   TEDC+I GYH+ + TRLIVN  K+QRD
Sbjct: 363 DESDLVKLTYIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAWKIQRD 422

Query: 439 PLVWEEPNEFRPERFLTTHKDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIHLTLANLLH 498
           PLVW +P+E++PERFL   +D D++GQ+ +LIPFG+GRR CP  S A Q++ LTLA++LH
Sbjct: 423 PLVWSQPHEYQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLTLAHILH 482

Query: 499 GFEIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPRL 524
           GFE+  P+++ +DM E+ G+   K +PLEV++ PR+
Sbjct: 483 GFELRTPNQNKVDMTETPGIVHAKATPLEVLVAPRI 513

BLAST of CSPI03G42320 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 1.4e-133
Identity = 236/517 (45.65%), Postives = 337/517 (65.18%), Query Frame = 0

Query: 18  MSLFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWPVIGHLHLLSSSEPGHKT 77
           +SL  L  FLY               R+ +  +  PV  GAWP++GHL LL+ S+  HK 
Sbjct: 19  LSLIFLCLFLY---------------RKNSRGKDAPVVSGAWPILGHLSLLNGSQTPHKV 78

Query: 78  LGKMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFASRPKLTSAKLLGYNNSMF 137
           LG +AD  GP+FT+KLG+  A+V+SNWE+++E  TTND+  +SRPKL + +++ YN +  
Sbjct: 79  LGALADKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFV 138

Query: 138 GLAQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINKLYQLCT------KEKPL 197
           GLA YGP+WR +RK+V+ E LS  R++Q   IR+SE+++SI +L+ + +          L
Sbjct: 139 GLAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTL 198

Query: 198 VEMKAWFEDITLNIMFKIIFGKRF-----TDDLKGDQDHRKTFRDLMELFGVFVPSDSLP 257
           V++K W   +T N++ +++ GKR+      +     Q   K  R+ M L G F  +D +P
Sbjct: 199 VDIKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGVP 258

Query: 258 FLSWLDLGGYEKAMKTTSKVLDEVFDKWLEEHRQRK-IENNDNGAEDFMDVMLSIIKDDD 317
            L WLDLGG+EKAMK  +K +D++  +WLEEHRQ+K +  N     DFMDVM+S +  + 
Sbjct: 259 CLRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQKKLLGENVESDRDFMDVMISAL--NG 318

Query: 318 EQLSGYVGDSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQVGRQK 377
            Q+  +  D++ KA  L +IL G+D+T  T+TW LSLLL N   L KA+ E++ Q+G+ +
Sbjct: 319 AQIGAFDADTICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDE 378

Query: 378 QVTESDVKNLIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVNIQKLQ 437
            + ESD+  L+YLQAIVKE+LRLYP  P S P E TE+C + GYHI   TRLI N+ K+ 
Sbjct: 379 YIRESDISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIH 438

Query: 438 RDPLVWEEPNEFRPERFLTTHKDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIHLTLANL 497
           RDP VW +P EF+PERFLTTHKD D+RG N +L+PFG+GRR+C G S    ++H TLANL
Sbjct: 439 RDPSVWSDPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLANL 498

Query: 498 LHGFEIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPR 523
           LH F+I  PS + +DM E  G T+TK +PLE+++ PR
Sbjct: 499 LHSFDILNPSAEPVDMTEFFGFTNTKATPLEILVKPR 518

BLAST of CSPI03G42320 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 8.9e-133
Identity = 236/504 (46.83%), Postives = 341/504 (67.66%), Query Frame = 0

Query: 29  TLFWLLNLTSLFTSQRRKNNLQPLPVARGAWPVIGHLHLLSSSEPGHKTLGKMADSNGPI 88
           +L +LL L      ++   + +  P   GAWP+ GHL LL  S+  HK LG +A+ +GP+
Sbjct: 17  SLIFLLCLFLYGPLKKVHGSSKEAPTVGGAWPIFGHLPLLIGSKSPHKALGALAEKHGPL 76

Query: 89  FTLKLGIHRAIVVSNWEIAQECLTTNDIIFASRPKLTSAKLLGYNNSMFGLAQYGPFWRH 148
           FT+KLG  +A+VVS+WE+A+EC TTND+  ++RPKL  A+L+ YNN+M  +A YGP+WR 
Sbjct: 77  FTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMCYNNAMLLVAPYGPYWRE 136

Query: 149 MRKVVSLELLSTHRLQQFQPIRISEIQSSINKLYQLCTKEK-----PLVEMKAWFEDITL 208
           +RK++  E+LS+ R++Q Q +R+SE+Q+SI +LY +   +K       VE+K WF     
Sbjct: 137 LRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDYASVELKQWFAQPIF 196

Query: 209 NIMFKIIFGKRFTDDLKGDQDHRKTFR---DLMELFGVFVPSDSLPFLSWLDLGGYEKAM 268
           N++ +++ GKRF      D+   K  +   + M L GVF   D++P+L WLD GGYEKAM
Sbjct: 197 NMVLRMVVGKRFLSATATDEKAEKCVKAVDEFMRLAGVFTVGDAIPYLRWLDFGGYEKAM 256

Query: 269 KTTSKVLDEVFDKWLEEHRQ-RKIENNDNGAEDFMDVMLSIIKDDDEQLSGYVGDSVIKA 328
           K T+K LD +  +WLEEHRQ R +    +GA+DFM+VMLS +  D + + G   D++IK+
Sbjct: 257 KETAKELDVMISEWLEEHRQKRALGEGVDGAQDFMNVMLSSL--DGKTIDGIDADTLIKS 316

Query: 329 NCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQVGRQKQVTESDVKNLIYLQ 388
             L +I AG++ + +T+ W + L+L N   L+  + EL+ QVG+ + + ESD+ NL+YLQ
Sbjct: 317 TVLTIIQAGTEASISTIIWAMCLILKNPLILEN-KAELDIQVGKDRCICESDISNLVYLQ 376

Query: 389 AIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVNIQKLQRDPLVWEEPNEFRP 448
           A+VKE+LRLY   P+S P E  EDC++ GYH+   TRLI NI K+  DP VW +P EF+P
Sbjct: 377 AVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLITNIWKIHTDPNVWSDPFEFKP 436

Query: 449 ERFLTTHKDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIHLTLANLLHGFEIDRPSKDLL 508
           +RFLTTHKD DV+G + QL+PFG+GRR+CPG SF  Q +HL LA+ LH FEI  PS + L
Sbjct: 437 DRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTVHLALASFLHSFEILNPSTEPL 496

Query: 509 DMEESVGLTSTKKSPLEVVLTPRL 524
           DM E+ G+T+TK +PLEV++ P L
Sbjct: 497 DMTEAFGVTNTKATPLEVLVKPCL 517

BLAST of CSPI03G42320 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 7.5e-132
Identity = 244/524 (46.56%), Postives = 343/524 (65.46%), Query Frame = 0

Query: 15  MISMSLFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWPVIGHLHLLSSSEP- 74
           + S+ + IL+F    LF           + +K      P   GAWP+IGHLHLL   E  
Sbjct: 5   LFSLFVPILVFVFIALF----------KKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQL 64

Query: 75  GHKTLGKMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFASRPKLTSAKLLGYN 134
            ++TLGKMAD  GP  +L+LG + A VVS++E+A++C T ND   ASRP   +AK +GYN
Sbjct: 65  LYRTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYN 124

Query: 135 NSMFGLAQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINKLYQLCTKE---KP 194
            ++FG A Y  FWR MRK+ ++ELLS  RLQ  + +R+SEI   +  LY L  K    KP
Sbjct: 125 FAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKP 184

Query: 195 -LVEMKAWFEDITLNIMFKIIFGKRF--------TDDLKGDQDHRKTFRDLMELFGVFVP 254
            +V++K+W ED+TLN++ +++ GKR+        ++D +     +K       L G+F  
Sbjct: 185 VMVDLKSWLEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTV 244

Query: 255 SDSLPFLSWLDLGGYEKAMKTTSKVLDEVFDKWLEEHR-QRKIENNDNGAEDFMDVMLSI 314
           SD+ P LS+ DL G+EK MK T   LD + ++W+E HR QRK         DF+DVM+S+
Sbjct: 245 SDAFPTLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSL 304

Query: 315 IKDDDEQLSGYVGDSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQ 374
            +        Y  ++ IK+ CLA+IL GSDT+ +T+TW +SLLLNN+E LKKAQ E++  
Sbjct: 305 AEQGKLSHLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIH 364

Query: 375 VGRQKQVTESDVKNLIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVN 434
           VGR + V +SD++NL+YLQAI+KE+LRLYPA P+  P E+ EDC++ GY++P  TRLIVN
Sbjct: 365 VGRDRNVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVN 424

Query: 435 IQKLQRDPLVWEEPNEFRPERFLTTH-KDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIH 494
           + K+QRDP V+ EPNEFRPERF+T   K+FDVRGQN +L+PFG+GRR CPG+S A Q++H
Sbjct: 425 VWKIQRDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLH 484

Query: 495 LTLANLLHGFEIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPRL 524
           L LA  LH F++       +DM E+ GLT  K +PLEV+++PR+
Sbjct: 485 LGLARFLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRI 518

BLAST of CSPI03G42320 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 466.1 bits (1198), Expect = 5.4e-130
Identity = 240/539 (44.53%), Postives = 344/539 (63.82%), Query Frame = 0

Query: 21  FILIFFLYTLFWLLNLTS----LFTSQRRKNNLQPLPVARGAWPVIGHLHLLSSSEPGHK 80
           F+L +   T    ++L S    LF   +  +  +P P+  G+WP++GHL L+ +++  HK
Sbjct: 1   FVLNYLNTTTIAFISLISLLFFLFRFSKVSHTKEP-PIISGSWPLLGHLPLMRNTQTPHK 60

Query: 81  TLGKMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFASRPKLTSAKLLGYNNSM 140
           TLG + D  GPIFT+KLG   A+V+SNWE+A+EC T NDI+ +SRPK  + +L+ YN + 
Sbjct: 61  TLGALVDKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAF 120

Query: 141 FGLAQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINKLYQL------------ 200
            G A YG +WR +RK+V+LE+LS  R++    IR+SE+Q+SI +L  +            
Sbjct: 121 IGWAPYGAYWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLL 180

Query: 201 ------CTKEKP------LVEMKAWFEDITLNIMFKIIFGKR-FTD-DLKGDQDHRK--- 260
                  T ++P       VE+K WF  +TLN++ +++ GKR F D D++  ++ ++   
Sbjct: 181 DDTKSSSTNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLE 240

Query: 261 TFRDLMELFGVFVPSDSLPFLSWLDLGGYEKAMKTTSKVLDEVFDKWLEEHRQRK---IE 320
             RD M L G F   D +PFL WLDLGG+EK MK  +K  D + ++WLEEHR++K    E
Sbjct: 241 NIRDFMRLIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGLGSE 300

Query: 321 NNDNGAEDFMDVMLSIIKDDDEQLSGYVGDSVIKANCLAMILAGSDTTTTTMTWTLSLLL 380
           +   G  DFMD ML ++K  D+ + G+  D++IKA  L +IL GSDTT  T+TW + LLL
Sbjct: 301 DKVVGERDFMDAMLLVLK--DKPIEGFDVDTIIKATTLELILGGSDTTAGTLTWAMCLLL 360

Query: 381 NNQETLKKAQIELEEQVGRQKQVTESDVKNLIYLQAIVKESLRLYPALPISIPHESTEDC 440
            +   L+K + EL   +G+++ V ESD+  L+YL AI+KE+LRLYP  P S P E TEDC
Sbjct: 361 KHPHVLEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDC 420

Query: 441 SIFGYHIPSRTRLIVNIQKLQRDPLVWEEPNEFRPERFLTTHKDFDVRGQNPQLIPFGNG 500
           +I GYHI   TRL+ N+ K+ RDP VW +P EF+PERFL+THKD DVRGQN +L+PFG+G
Sbjct: 421 TIGGYHIKKGTRLMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLPFGSG 480

Query: 501 RRMCPGTSFAFQIIHLTLANLLHGFEIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPRL 524
           RRMC G S    ++H  LAN LH FEI  PS + +D+ E +   +TK +PLEV++ P L
Sbjct: 481 RRMCAGMSLGLHMVHYILANFLHSFEILNPSPESIDVTEVLEFVTTKATPLEVLVKPCL 536

BLAST of CSPI03G42320 vs. ExPASy TrEMBL
Match: A0A0A0LGG2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G853150 PE=3 SV=1)

HSP 1 Score: 1054.7 bits (2726), Expect = 1.3e-304
Identity = 528/529 (99.81%), Postives = 529/529 (100.00%), Query Frame = 0

Query: 1   MELQFSASFSSPKIMISMSLFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWP 60
           MELQFSASFSSPKIMISMSLFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWP
Sbjct: 1   MELQFSASFSSPKIMISMSLFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWP 60

Query: 61  VIGHLHLLSSSEPGHKTLGKMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFAS 120
           VIGHLHLLSSSEPGHKTLGKMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFAS
Sbjct: 61  VIGHLHLLSSSEPGHKTLGKMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFAS 120

Query: 121 RPKLTSAKLLGYNNSMFGLAQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINK 180
           RPKLTSAKLLGYNNSMFGLAQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINK
Sbjct: 121 RPKLTSAKLLGYNNSMFGLAQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINK 180

Query: 181 LYQLCTKEKPLVEMKAWFEDITLNIMFKIIFGKRFTDDLKGDQDHRKTFRDLMELFGVFV 240
           LYQLCTKEKPLVEMKAWFEDITLNIMFKIIFGKRFTDDLKGDQDHRKTFR+LMELFGVFV
Sbjct: 181 LYQLCTKEKPLVEMKAWFEDITLNIMFKIIFGKRFTDDLKGDQDHRKTFRNLMELFGVFV 240

Query: 241 PSDSLPFLSWLDLGGYEKAMKTTSKVLDEVFDKWLEEHRQRKIENNDNGAEDFMDVMLSI 300
           PSDSLPFLSWLDLGGYEKAMKTTSKVLDEVFDKWLEEHRQRKIENNDNGAEDFMDVMLSI
Sbjct: 241 PSDSLPFLSWLDLGGYEKAMKTTSKVLDEVFDKWLEEHRQRKIENNDNGAEDFMDVMLSI 300

Query: 301 IKDDDEQLSGYVGDSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQ 360
           IKDDDEQLSGYVGDSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQ
Sbjct: 301 IKDDDEQLSGYVGDSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQ 360

Query: 361 VGRQKQVTESDVKNLIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVN 420
           VGRQKQVTESDVKNLIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVN
Sbjct: 361 VGRQKQVTESDVKNLIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVN 420

Query: 421 IQKLQRDPLVWEEPNEFRPERFLTTHKDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIHL 480
           IQKLQRDPLVWEEPNEFRPERFLTTHKDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIHL
Sbjct: 421 IQKLQRDPLVWEEPNEFRPERFLTTHKDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIHL 480

Query: 481 TLANLLHGFEIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPRLPALGSH 530
           TLANLLHGFEIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPRLPALGSH
Sbjct: 481 TLANLLHGFEIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPRLPALGSH 529

BLAST of CSPI03G42320 vs. ExPASy TrEMBL
Match: A0A6J1FKD2 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111446070 PE=3 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 2.0e-188
Identity = 337/509 (66.21%), Postives = 408/509 (80.16%), Query Frame = 0

Query: 20  LFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWPVIGHLHLLSSSEPGHKTLG 79
           +F L+ FLY LF   N++   +S  ++  L P P   GAWPVIGHLHLLS++EP H TL 
Sbjct: 15  IFALLIFLYVLF---NISKRLSSNPKR--LPPEPA--GAWPVIGHLHLLSTTEPAHITLA 74

Query: 80  KMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFASRPKLTSAKLLGYNNSMFGL 139
           KMAD  GPIFTL+LG+H+A++VS+WEIA+EC TT D IFASRPKL ++KLLGYN +M GL
Sbjct: 75  KMADVYGPIFTLRLGMHKALIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMMGL 134

Query: 140 AQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINKLYQLC--TKEKPLVEMKAW 199
           + YG  WRH+RK+ +LEL + HRLQQ Q +R+SE+ SSI KLY+LC   KEK LV+M  W
Sbjct: 135 SPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEVDSSIKKLYELCGNGKEKALVDMTTW 194

Query: 200 FEDITLNIMFKIIFGKRFTDDLK--GDQDHRKTFRDLMELFGVFVPSDSLPFLSWLDLGG 259
           F DITLN +F+++ GKRF+  ++  G+ ++RK  R+  E FGVFVPSDS PFL WLDLGG
Sbjct: 195 FGDITLNTIFRVVVGKRFSTAVEGGGNDEYRKALREFFEWFGVFVPSDSFPFLKWLDLGG 254

Query: 260 YEKAMKTTSKVLDEVFDKWLEEHRQRKIENNDNGAE-DFMDVMLSIIKDDDEQLSGYVGD 319
           +EKAMK T++VLDEVFDKW+EE R+ +  +    AE DFMDVMLS +KD DE LS Y  +
Sbjct: 255 HEKAMKKTAEVLDEVFDKWVEERRKMRASDEAKTAEHDFMDVMLSTVKDCDE-LSSYDAN 314

Query: 320 SVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQVGRQKQVTESDVKN 379
            VIK+ CLA+ILAGSDTTT TM W LSLLLNN E L+KA++EL+EQVGR+  V ESDVKN
Sbjct: 315 IVIKSTCLALILAGSDTTTVTMIWALSLLLNNPEALEKARLELDEQVGRETHVKESDVKN 374

Query: 380 LIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVNIQKLQRDPLVWEEP 439
           LIYLQAIVKE+LRLYPA P+ IPHES EDC+I  YHIP+ TRL+VNIQKLQRDP VWE+P
Sbjct: 375 LIYLQAIVKETLRLYPAGPLGIPHESIEDCTINDYHIPAGTRLMVNIQKLQRDPRVWEDP 434

Query: 440 NEFRPERFLTTHKDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIHLTLANLLHGFEIDRP 499
            EFRPERFLT H+DF+VRGQ+P+ IPFGNGRRMCPG SFA QI+HLTLANLLHGFEIDRP
Sbjct: 435 CEFRPERFLTNHRDFNVRGQSPEFIPFGNGRRMCPGISFALQIMHLTLANLLHGFEIDRP 494

Query: 500 SKDLLDMEESVGLTSTKKSPLEVVLTPRL 524
           SK+LL+MEES GLTS +K PLEVVL+PRL
Sbjct: 495 SKELLNMEESAGLTSIRKDPLEVVLSPRL 515

BLAST of CSPI03G42320 vs. ExPASy TrEMBL
Match: A0A5D3BHP3 (Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold156G001020 PE=3 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 4.5e-188
Identity = 344/538 (63.94%), Postives = 419/538 (77.88%), Query Frame = 0

Query: 1   MELQFS-ASFSSPKIMISMSLFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAW 60
           MEL  + AS     + I +++F+ + F Y LF +L      +S +    LQP P A GAW
Sbjct: 1   MELLLALASSPHTGVGIMIAIFLFVLFAYNLFRIL------SSSQHGKKLQP-PEAGGAW 60

Query: 61  PVIGHLHLLSSSEPGHKTLGKMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFA 120
           PVIGHLHLL  SEP HK L  MAD++GPIFTLKLG+HRA+VVSNWEIA+EC TTND IFA
Sbjct: 61  PVIGHLHLLGGSEPLHKVLAAMADAHGPIFTLKLGMHRAVVVSNWEIAKECFTTNDRIFA 120

Query: 121 SRPKLTSAKLLGYNNSMFGLAQYGPFWRHMRKVVSLELLSTHRL-QQFQPIRISEIQSSI 180
           SRPKLT++KLLGYNNSMFG ++YGP+WRHMRK+    LL++H + QQ Q I  SEIQSS+
Sbjct: 121 SRPKLTASKLLGYNNSMFGFSEYGPYWRHMRKIAVHGLLASHYMKQQLQRILESEIQSSV 180

Query: 181 NKLYQL-------CTKEKPLVEMKAWFEDITLNIMFKIIFGKRFTDDLKG--DQDHRKTF 240
            KLY L         +E  LVEM  WF+DITLN MF+++ GKRF+ DL+G  +QD+RK F
Sbjct: 181 GKLYDLWANHTKRSEQESLLVEMNTWFQDITLNTMFRMVVGKRFSTDLEGSRNQDYRKVF 240

Query: 241 RDLMELFGVFVPSDSLPFLSWLDLGGYEKAMKTTSKVLDEVFDKWLEEHRQRKIENNDNG 300
           RD ++ FG FVPSDS PFLSWLDLGGYEKAMK TS+ LDEVFD W+ E +     NN  G
Sbjct: 241 RDFVKFFGDFVPSDSFPFLSWLDLGGYEKAMKKTSEALDEVFDNWIREKK-----NNSGG 300

Query: 301 AE-DFMDVMLSIIKDDDEQLSGYVGDSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQE 360
            + DFMD+MLS + + DE+LS Y GDSV+KANCL+MILAGSDTTTTTM W LSLLLNN+E
Sbjct: 301 HQLDFMDLMLSAV-EKDEELSDYDGDSVVKANCLSMILAGSDTTTTTMIWALSLLLNNEE 360

Query: 361 TLKKAQIELEEQVGRQKQVTESDVKNLIYLQAIVKESLRLYPALPISIPHESTEDCSIFG 420
            LKK Q+ELEEQ+GRQ++V  +D+ +LIYLQAIVKE+LRLYPA P+S+PHESTEDC+I G
Sbjct: 361 ALKKVQLELEEQIGRQRKVKATDLNDLIYLQAIVKETLRLYPAAPLSVPHESTEDCNILG 420

Query: 421 YHIPSRTRLIVNIQKLQRDPLVWEEPNEFRPERFLTTHKDFDVRGQ-NPQLIPFGNGRRM 480
           Y+I +RTRLIVN+QKLQRDPLVW++PNEF+PERFLT  KD D +GQ NPQLIPFG+GRR 
Sbjct: 421 YNISARTRLIVNLQKLQRDPLVWKDPNEFQPERFLTAKKDLDFKGQNNPQLIPFGSGRRA 480

Query: 481 CPGTSFAFQIIHLTLANLLHGFEIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPRLPA 526
           CPG SFA +++ LTLANL+HGFEI RPS +LL MEE+ G  S +K+PL+VVLTPRL A
Sbjct: 481 CPGMSFALEVVPLTLANLIHGFEIGRPSNELLKMEENFGFISVRKAPLQVVLTPRLSA 525

BLAST of CSPI03G42320 vs. ExPASy TrEMBL
Match: A0A1S3CM83 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502578 PE=3 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 4.5e-188
Identity = 344/538 (63.94%), Postives = 419/538 (77.88%), Query Frame = 0

Query: 1   MELQFS-ASFSSPKIMISMSLFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAW 60
           MEL  + AS     + I +++F+ + F Y LF +L      +S +    LQP P A GAW
Sbjct: 1   MELLLALASSPHTGVGIMIAIFLFVLFAYNLFRIL------SSSQHGKKLQP-PEAGGAW 60

Query: 61  PVIGHLHLLSSSEPGHKTLGKMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFA 120
           PVIGHLHLL  SEP HK L  MAD++GPIFTLKLG+HRA+VVSNWEIA+EC TTND IFA
Sbjct: 61  PVIGHLHLLGGSEPLHKVLAAMADAHGPIFTLKLGMHRAVVVSNWEIAKECFTTNDRIFA 120

Query: 121 SRPKLTSAKLLGYNNSMFGLAQYGPFWRHMRKVVSLELLSTHRL-QQFQPIRISEIQSSI 180
           SRPKLT++KLLGYNNSMFG ++YGP+WRHMRK+    LL++H + QQ Q I  SEIQSS+
Sbjct: 121 SRPKLTASKLLGYNNSMFGFSEYGPYWRHMRKIAVHGLLASHYMKQQLQRILESEIQSSV 180

Query: 181 NKLYQL-------CTKEKPLVEMKAWFEDITLNIMFKIIFGKRFTDDLKG--DQDHRKTF 240
            KLY L         +E  LVEM  WF+DITLN MF+++ GKRF+ DL+G  +QD+RK F
Sbjct: 181 GKLYDLWANHTKRSEQESLLVEMNTWFQDITLNTMFRMVVGKRFSTDLEGSRNQDYRKVF 240

Query: 241 RDLMELFGVFVPSDSLPFLSWLDLGGYEKAMKTTSKVLDEVFDKWLEEHRQRKIENNDNG 300
           RD ++ FG FVPSDS PFLSWLDLGGYEKAMK TS+ LDEVFD W+ E +     NN  G
Sbjct: 241 RDFVKFFGDFVPSDSFPFLSWLDLGGYEKAMKKTSEALDEVFDNWIREKK-----NNSGG 300

Query: 301 AE-DFMDVMLSIIKDDDEQLSGYVGDSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQE 360
            + DFMD+MLS + + DE+LS Y GDSV+KANCL+MILAGSDTTTTTM W LSLLLNN+E
Sbjct: 301 HQLDFMDLMLSAV-EKDEELSDYDGDSVVKANCLSMILAGSDTTTTTMIWALSLLLNNEE 360

Query: 361 TLKKAQIELEEQVGRQKQVTESDVKNLIYLQAIVKESLRLYPALPISIPHESTEDCSIFG 420
            LKK Q+ELEEQ+GRQ++V  +D+ +LIYLQAIVKE+LRLYPA P+S+PHESTEDC+I G
Sbjct: 361 ALKKVQLELEEQIGRQRKVKATDLNDLIYLQAIVKETLRLYPAAPLSVPHESTEDCNILG 420

Query: 421 YHIPSRTRLIVNIQKLQRDPLVWEEPNEFRPERFLTTHKDFDVRGQ-NPQLIPFGNGRRM 480
           Y+I +RTRLIVN+QKLQRDPLVW++PNEF+PERFLT  KD D +GQ NPQLIPFG+GRR 
Sbjct: 421 YNISARTRLIVNLQKLQRDPLVWKDPNEFQPERFLTAKKDLDFKGQNNPQLIPFGSGRRA 480

Query: 481 CPGTSFAFQIIHLTLANLLHGFEIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPRLPA 526
           CPG SFA +++ LTLANL+HGFEI RPS +LL MEE+ G  S +K+PL+VVLTPRL A
Sbjct: 481 CPGMSFALEVVPLTLANLIHGFEIGRPSNELLKMEENFGFISVRKAPLQVVLTPRLSA 525

BLAST of CSPI03G42320 vs. ExPASy TrEMBL
Match: A0A6J1J4B0 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111481116 PE=3 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.7e-187
Identity = 333/509 (65.42%), Postives = 408/509 (80.16%), Query Frame = 0

Query: 20  LFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWPVIGHLHLLSSSEPGHKTLG 79
           +F L+ FLY LF   N+++  +S  ++  L P P   GAWPVIGHLHLLS++EP H TL 
Sbjct: 18  IFTLLIFLYALF---NISTKLSSNPKR--LPPEPA--GAWPVIGHLHLLSTTEPAHITLA 77

Query: 80  KMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFASRPKLTSAKLLGYNNSMFGL 139
           KMAD  GPIFTLKLG+HR ++VS+WEIA+EC TT D IFASRPKL ++KLLGYN +M GL
Sbjct: 78  KMADVYGPIFTLKLGMHRTLIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMMGL 137

Query: 140 AQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINKLYQLC--TKEKPLVEMKAW 199
           + YG  WRH+RK+ +LEL + HRLQQ Q +R+SE++SSI KLY+LC   KEK LV+MK W
Sbjct: 138 SPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEVESSIKKLYELCGNGKEKALVDMKTW 197

Query: 200 FEDITLNIMFKIIFGKRFTDDLK--GDQDHRKTFRDLMELFGVFVPSDSLPFLSWLDLGG 259
           F DITLN +F+++ GKRF+  ++  G++++RK  R+  E FGVFVPSDS PFL WLDLGG
Sbjct: 198 FGDITLNTIFRVVVGKRFSTAVEGGGNEEYRKALREFFEWFGVFVPSDSFPFLKWLDLGG 257

Query: 260 YEKAMKTTSKVLDEVFDKWLEEHRQRKIENNDNGAE-DFMDVMLSIIKDDDEQLSGYVGD 319
           +EKAMK T++VLDEV DKW+EE R+ +  +    AE DFMDVMLS +K D E+LS Y  +
Sbjct: 258 HEKAMKKTAEVLDEVLDKWVEERRKMRASDEAKTAEHDFMDVMLSGVK-DYEELSSYEAN 317

Query: 320 SVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQVGRQKQVTESDVKN 379
           +VIK+ CLA+ILAGSDTTT TM W LSLLLNN E LKKAQ+EL+E VGR+  + ESDVKN
Sbjct: 318 TVIKSTCLALILAGSDTTTVTMVWALSLLLNNPEALKKAQLELDEHVGRETHLKESDVKN 377

Query: 380 LIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVNIQKLQRDPLVWEEP 439
           L+YLQAIVKE+LRLYPA P+ IPHES EDC+I  YHIP+ TRL+VNIQKLQRDP VWE+P
Sbjct: 378 LVYLQAIVKETLRLYPAGPLGIPHESIEDCTINDYHIPAGTRLMVNIQKLQRDPQVWEDP 437

Query: 440 NEFRPERFLTTHKDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIHLTLANLLHGFEIDRP 499
           +EFRPERFLT+H+DF+ RGQ P+ IPFGNGRRMCPG SFA QI+HLTLANLLH FEIDRP
Sbjct: 438 SEFRPERFLTSHRDFNFRGQTPEFIPFGNGRRMCPGISFALQIMHLTLANLLHEFEIDRP 497

Query: 500 SKDLLDMEESVGLTSTKKSPLEVVLTPRL 524
           SK+LL+MEES G+TS +K PLEVVL PRL
Sbjct: 498 SKELLNMEESAGMTSIRKDPLEVVLRPRL 518

BLAST of CSPI03G42320 vs. NCBI nr
Match: XP_004147801.1 (cytochrome P450 CYP82D47 [Cucumis sativus] >KGN59917.1 hypothetical protein Csa_001281 [Cucumis sativus])

HSP 1 Score: 1054.7 bits (2726), Expect = 2.7e-304
Identity = 528/529 (99.81%), Postives = 529/529 (100.00%), Query Frame = 0

Query: 1   MELQFSASFSSPKIMISMSLFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWP 60
           MELQFSASFSSPKIMISMSLFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWP
Sbjct: 1   MELQFSASFSSPKIMISMSLFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWP 60

Query: 61  VIGHLHLLSSSEPGHKTLGKMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFAS 120
           VIGHLHLLSSSEPGHKTLGKMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFAS
Sbjct: 61  VIGHLHLLSSSEPGHKTLGKMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFAS 120

Query: 121 RPKLTSAKLLGYNNSMFGLAQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINK 180
           RPKLTSAKLLGYNNSMFGLAQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINK
Sbjct: 121 RPKLTSAKLLGYNNSMFGLAQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINK 180

Query: 181 LYQLCTKEKPLVEMKAWFEDITLNIMFKIIFGKRFTDDLKGDQDHRKTFRDLMELFGVFV 240
           LYQLCTKEKPLVEMKAWFEDITLNIMFKIIFGKRFTDDLKGDQDHRKTFR+LMELFGVFV
Sbjct: 181 LYQLCTKEKPLVEMKAWFEDITLNIMFKIIFGKRFTDDLKGDQDHRKTFRNLMELFGVFV 240

Query: 241 PSDSLPFLSWLDLGGYEKAMKTTSKVLDEVFDKWLEEHRQRKIENNDNGAEDFMDVMLSI 300
           PSDSLPFLSWLDLGGYEKAMKTTSKVLDEVFDKWLEEHRQRKIENNDNGAEDFMDVMLSI
Sbjct: 241 PSDSLPFLSWLDLGGYEKAMKTTSKVLDEVFDKWLEEHRQRKIENNDNGAEDFMDVMLSI 300

Query: 301 IKDDDEQLSGYVGDSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQ 360
           IKDDDEQLSGYVGDSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQ
Sbjct: 301 IKDDDEQLSGYVGDSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQ 360

Query: 361 VGRQKQVTESDVKNLIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVN 420
           VGRQKQVTESDVKNLIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVN
Sbjct: 361 VGRQKQVTESDVKNLIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVN 420

Query: 421 IQKLQRDPLVWEEPNEFRPERFLTTHKDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIHL 480
           IQKLQRDPLVWEEPNEFRPERFLTTHKDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIHL
Sbjct: 421 IQKLQRDPLVWEEPNEFRPERFLTTHKDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIHL 480

Query: 481 TLANLLHGFEIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPRLPALGSH 530
           TLANLLHGFEIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPRLPALGSH
Sbjct: 481 TLANLLHGFEIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPRLPALGSH 529

BLAST of CSPI03G42320 vs. NCBI nr
Match: XP_038905158.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 837.4 bits (2162), Expect = 6.8e-239
Identity = 420/531 (79.10%), Postives = 465/531 (87.57%), Query Frame = 0

Query: 1   MELQFSASFSSPKIMISMSLFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWP 60
           M+L FSASFSS K MI   LF+LIFFLYTLFW+L+ +SLF+SQ R    QP PVA GAWP
Sbjct: 3   MKLPFSASFSSAKTMIMSVLFLLIFFLYTLFWVLSKSSLFSSQHRNKKPQPPPVAGGAWP 62

Query: 61  VIGHLHLLSSSEPGHKTLGKMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFAS 120
           VIGHLHLL+ SEP HK +GKMADS GPIFTLKLG H A+VVS+WEIA+EC TTND  FAS
Sbjct: 63  VIGHLHLLNGSEPAHKIIGKMADSTGPIFTLKLGTHTAVVVSDWEIAKECFTTNDRAFAS 122

Query: 121 RPKLTSAKLLGYNNSMFGLAQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINK 180
           RPKLT+ K + Y+NSMFG AQYGPFWRHMRK+ SLELLS HRL QFQPIR SEIQSSI K
Sbjct: 123 RPKLTATKHMAYDNSMFGFAQYGPFWRHMRKITSLELLSNHRLHQFQPIRTSEIQSSIKK 182

Query: 181 LYQLCT----KEKPLVEMKAWFEDITLNIMFKIIFGKRFTD-DLKGDQDHRKTFRDLMEL 240
           LY+LCT     EK LV+MK WFEDITLNI+FKI+FGKRF+D  L+G +D+RKTFR L+EL
Sbjct: 183 LYELCTINKNGEKVLVDMKTWFEDITLNIIFKIVFGKRFSDAKLEGSEDYRKTFRGLLEL 242

Query: 241 FGVFVPSDSLPFLSWLDLGGYEKAMKTTSKVLDEVFDKWLEEHRQRKIENNDNGA-EDFM 300
           FG+FVPSDS PFLSWLDLGGYEKAMK  SKV+DEVFDKWL+EHRQRK+E+N+N   +DFM
Sbjct: 243 FGMFVPSDSFPFLSWLDLGGYEKAMKKASKVVDEVFDKWLKEHRQRKMESNNNKVDQDFM 302

Query: 301 DVMLSIIKDDDEQLSGYVGDSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQ 360
           DVM+SI+KDDDEQLSGY GDSVIKA CLAMIL G DTT TTMTW LSLLLNNQETLKKA+
Sbjct: 303 DVMISIVKDDDEQLSGYDGDSVIKATCLAMILGGFDTTATTMTWALSLLLNNQETLKKAK 362

Query: 361 IELEEQVGRQKQVTESDVKNLIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSR 420
           +ELEEQVGRQKQV ESDVKNLIYLQAIVKE+LRLYPA P+SIP ES EDCSIFGYHIPSR
Sbjct: 363 LELEEQVGRQKQVRESDVKNLIYLQAIVKETLRLYPAAPLSIPRESIEDCSIFGYHIPSR 422

Query: 421 TRLIVNIQKLQRDPLVWEEPNEFRPERFLTTHKDFDVRGQNPQLIPFGNGRRMCPGTSFA 480
           TRLIVN+QKLQRDPLVWEEPNEF+PERFLT+ K+F VRGQ+PQLIPFG+GRR+C G SFA
Sbjct: 423 TRLIVNLQKLQRDPLVWEEPNEFQPERFLTSKKEFFVRGQSPQLIPFGSGRRICLGISFA 482

Query: 481 FQIIHLTLANLLHGFEIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPRLPA 526
            Q+IHLTLANLLH FEIDRPSKDLLDMEES GLTSTKKSPLEVVLTPRLPA
Sbjct: 483 LQVIHLTLANLLHEFEIDRPSKDLLDMEESFGLTSTKKSPLEVVLTPRLPA 533

BLAST of CSPI03G42320 vs. NCBI nr
Match: XP_038903388.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 688.3 bits (1775), Expect = 5.1e-194
Identity = 347/519 (66.86%), Postives = 418/519 (80.54%), Query Frame = 0

Query: 16  ISMSLFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWPVIGHLHLLSSSEPGH 75
           I M + +LI F Y L W ++   L +S+ RK   QP P A GA  VIGHLHLL  SEP H
Sbjct: 17  IMMPVILLILFFYALLWFISRRLLLSSEHRKKP-QP-PEASGALLVIGHLHLLGGSEPAH 76

Query: 76  KTLGKMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFASRPKLTSAKLLGYNNS 135
           K LG MAD++GPIFTLKLG+H+A+VVS+WEIA+ECLTTND IFASRPKLT+AKLLGYNNS
Sbjct: 77  KILGAMADAHGPIFTLKLGVHKAVVVSSWEIAKECLTTNDRIFASRPKLTAAKLLGYNNS 136

Query: 136 MFGLAQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINKLYQLCTKEKP----- 195
           MFG ++YGPFWRHMRK+  L LL+ +  +Q + I  SEI+SSI KLY L    K      
Sbjct: 137 MFGFSEYGPFWRHMRKITMLGLLTNNHKKQLELIVESEIRSSIGKLYDLWVNHKKSKGEA 196

Query: 196 --LVEMKAWFEDITLNIMFKIIFGKRFTDDLK--GDQDHRKTFRDLMELFGVFVPSDSLP 255
             LVEMK+WFEDITLN MF+++ GKRF+ DL+  G+QD+RK FR+ + LFG FVPSDS P
Sbjct: 197 LLLVEMKSWFEDITLNTMFRMVVGKRFSTDLEGSGNQDYRKVFREFVNLFGDFVPSDSFP 256

Query: 256 FLSWLDLGGYEKAMKTTSKVLDEVFDKWLEEHRQRKIENNDNGAEDFMDVMLSIIKDDDE 315
           FLS  DLGGYEK+MK T+KVLDEV DKW++E +     N+ +  +DFMD +LS +K DDE
Sbjct: 257 FLSCFDLGGYEKSMKKTAKVLDEVLDKWIKEKK-----NSGDHEQDFMDFLLSAVK-DDE 316

Query: 316 QLSGYVGDSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQVGRQKQ 375
           +LSGY GDS++KANCL++ILAGSDTT TTM W LSLLLNN+ETLKKAQ+ELEEQVGR++Q
Sbjct: 317 ELSGYDGDSIVKANCLSLILAGSDTTATTMIWALSLLLNNEETLKKAQLELEEQVGRRRQ 376

Query: 376 VTESDVKNLIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVNIQKLQR 435
           V  +D+ +LIYL+AIVKE+LRLYPA P+S+PHESTEDC+I GY+IP+RTRLIVN+QKLQR
Sbjct: 377 VKVADINDLIYLEAIVKETLRLYPAAPLSVPHESTEDCNILGYYIPARTRLIVNLQKLQR 436

Query: 436 DPLVWEEPNEFRPERFLTTHKDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIHLTLANLL 495
           DPLVWE+PN+F+PERFLT  KD DV+GQNPQLIPFG+GRR CPG SFA QI+ L LAN +
Sbjct: 437 DPLVWEDPNKFQPERFLTDQKDLDVKGQNPQLIPFGSGRRTCPGISFALQIMPLVLANFI 496

Query: 496 HGFEIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPRLPA 526
           HGFEI RPSK+LLDMEESVGL S +K PLEVVLTPRL A
Sbjct: 497 HGFEIVRPSKELLDMEESVGLISVRKDPLEVVLTPRLSA 527

BLAST of CSPI03G42320 vs. NCBI nr
Match: XP_023523452.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 669.8 bits (1727), Expect = 1.9e-188
Identity = 337/509 (66.21%), Postives = 408/509 (80.16%), Query Frame = 0

Query: 20  LFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWPVIGHLHLLSSSEPGHKTLG 79
           +F L+ FLY LF   N+    +S  ++  L P P   GAWPVIGHLHLLS++EP H TL 
Sbjct: 15  IFALLIFLYALF---NIYKRLSSNPKR--LPPEPA--GAWPVIGHLHLLSTTEPAHITLA 74

Query: 80  KMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFASRPKLTSAKLLGYNNSMFGL 139
           KMAD  GPIFTL+LG+H+A++VS+WEIA+EC TT D IFASRPKL ++KLLGYN +M GL
Sbjct: 75  KMADVYGPIFTLRLGMHKALIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMMGL 134

Query: 140 AQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINKLYQLC--TKEKPLVEMKAW 199
           + YG  WRH+RK+ +LEL + HRLQQ Q +R+SE++S+I KLY LC   KEK LV+MK W
Sbjct: 135 SPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEVESAIKKLYALCGNGKEKALVDMKTW 194

Query: 200 FEDITLNIMFKIIFGKRFTDDLK--GDQDHRKTFRDLMELFGVFVPSDSLPFLSWLDLGG 259
           F DITLN +F+++ GKRF+  ++  G+ ++RK  R+  E FGVFVPSDS PFL WLDLGG
Sbjct: 195 FGDITLNTIFRVVVGKRFSTAVEGGGNDEYRKALREFFEWFGVFVPSDSFPFLKWLDLGG 254

Query: 260 YEKAMKTTSKVLDEVFDKWLEEHRQRKIENNDNGAE-DFMDVMLSIIKDDDEQLSGYVGD 319
           +EKAMK T++VLD+VFDKW+EE R+ +  +    AE DFMDVMLS +KD DE LS Y  +
Sbjct: 255 HEKAMKKTAEVLDDVFDKWVEERRKMRASDEAKTAEHDFMDVMLSTVKDYDE-LSSYDAN 314

Query: 320 SVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQVGRQKQVTESDVKN 379
           +VIK+ CLA+ILAGSDTTT TM W LSLLLNN E LKKAQIEL+E VGRQKQV ESDVKN
Sbjct: 315 TVIKSTCLALILAGSDTTTVTMIWALSLLLNNPEALKKAQIELDEHVGRQKQVKESDVKN 374

Query: 380 LIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVNIQKLQRDPLVWEEP 439
           L+YLQAIVKE+LRLYP  P  IPHESTEDC+I  YHIP+RTRL+VNIQKLQRDP VWE+P
Sbjct: 375 LVYLQAIVKETLRLYPPGPFGIPHESTEDCTINDYHIPARTRLMVNIQKLQRDPRVWEDP 434

Query: 440 NEFRPERFLTTHKDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIHLTLANLLHGFEIDRP 499
            EF+PERFLT H+DFDVRGQ+P+ IPFGNGRRMCPG SFA QI+HLTLANLLH FEIDR 
Sbjct: 435 CEFQPERFLTRHRDFDVRGQSPEFIPFGNGRRMCPGISFALQIMHLTLANLLHRFEIDRS 494

Query: 500 SKDLLDMEESVGLTSTKKSPLEVVLTPRL 524
           SK+L++MEES G+TS +K PLEVVL+PRL
Sbjct: 495 SKELMNMEESAGMTSIRKGPLEVVLSPRL 515

BLAST of CSPI03G42320 vs. NCBI nr
Match: XP_022940489.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 668.7 bits (1724), Expect = 4.2e-188
Identity = 337/509 (66.21%), Postives = 408/509 (80.16%), Query Frame = 0

Query: 20  LFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWPVIGHLHLLSSSEPGHKTLG 79
           +F L+ FLY LF   N++   +S  ++  L P P   GAWPVIGHLHLLS++EP H TL 
Sbjct: 15  IFALLIFLYVLF---NISKRLSSNPKR--LPPEPA--GAWPVIGHLHLLSTTEPAHITLA 74

Query: 80  KMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFASRPKLTSAKLLGYNNSMFGL 139
           KMAD  GPIFTL+LG+H+A++VS+WEIA+EC TT D IFASRPKL ++KLLGYN +M GL
Sbjct: 75  KMADVYGPIFTLRLGMHKALIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMMGL 134

Query: 140 AQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINKLYQLC--TKEKPLVEMKAW 199
           + YG  WRH+RK+ +LEL + HRLQQ Q +R+SE+ SSI KLY+LC   KEK LV+M  W
Sbjct: 135 SPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEVDSSIKKLYELCGNGKEKALVDMTTW 194

Query: 200 FEDITLNIMFKIIFGKRFTDDLK--GDQDHRKTFRDLMELFGVFVPSDSLPFLSWLDLGG 259
           F DITLN +F+++ GKRF+  ++  G+ ++RK  R+  E FGVFVPSDS PFL WLDLGG
Sbjct: 195 FGDITLNTIFRVVVGKRFSTAVEGGGNDEYRKALREFFEWFGVFVPSDSFPFLKWLDLGG 254

Query: 260 YEKAMKTTSKVLDEVFDKWLEEHRQRKIENNDNGAE-DFMDVMLSIIKDDDEQLSGYVGD 319
           +EKAMK T++VLDEVFDKW+EE R+ +  +    AE DFMDVMLS +KD DE LS Y  +
Sbjct: 255 HEKAMKKTAEVLDEVFDKWVEERRKMRASDEAKTAEHDFMDVMLSTVKDCDE-LSSYDAN 314

Query: 320 SVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQVGRQKQVTESDVKN 379
            VIK+ CLA+ILAGSDTTT TM W LSLLLNN E L+KA++EL+EQVGR+  V ESDVKN
Sbjct: 315 IVIKSTCLALILAGSDTTTVTMIWALSLLLNNPEALEKARLELDEQVGRETHVKESDVKN 374

Query: 380 LIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVNIQKLQRDPLVWEEP 439
           LIYLQAIVKE+LRLYPA P+ IPHES EDC+I  YHIP+ TRL+VNIQKLQRDP VWE+P
Sbjct: 375 LIYLQAIVKETLRLYPAGPLGIPHESIEDCTINDYHIPAGTRLMVNIQKLQRDPRVWEDP 434

Query: 440 NEFRPERFLTTHKDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIHLTLANLLHGFEIDRP 499
            EFRPERFLT H+DF+VRGQ+P+ IPFGNGRRMCPG SFA QI+HLTLANLLHGFEIDRP
Sbjct: 435 CEFRPERFLTNHRDFNVRGQSPEFIPFGNGRRMCPGISFALQIMHLTLANLLHGFEIDRP 494

Query: 500 SKDLLDMEESVGLTSTKKSPLEVVLTPRL 524
           SK+LL+MEES GLTS +K PLEVVL+PRL
Sbjct: 495 SKELLNMEESAGLTSIRKDPLEVVLSPRL 515

BLAST of CSPI03G42320 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 472.2 bits (1214), Expect = 5.3e-133
Identity = 244/524 (46.56%), Postives = 343/524 (65.46%), Query Frame = 0

Query: 15  MISMSLFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWPVIGHLHLLSSSEP- 74
           + S+ + IL+F    LF           + +K      P   GAWP+IGHLHLL   E  
Sbjct: 5   LFSLFVPILVFVFIALF----------KKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQL 64

Query: 75  GHKTLGKMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFASRPKLTSAKLLGYN 134
            ++TLGKMAD  GP  +L+LG + A VVS++E+A++C T ND   ASRP   +AK +GYN
Sbjct: 65  LYRTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYN 124

Query: 135 NSMFGLAQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINKLYQLCTKE---KP 194
            ++FG A Y  FWR MRK+ ++ELLS  RLQ  + +R+SEI   +  LY L  K    KP
Sbjct: 125 FAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKP 184

Query: 195 -LVEMKAWFEDITLNIMFKIIFGKRF--------TDDLKGDQDHRKTFRDLMELFGVFVP 254
            +V++K+W ED+TLN++ +++ GKR+        ++D +     +K       L G+F  
Sbjct: 185 VMVDLKSWLEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTV 244

Query: 255 SDSLPFLSWLDLGGYEKAMKTTSKVLDEVFDKWLEEHR-QRKIENNDNGAEDFMDVMLSI 314
           SD+ P LS+ DL G+EK MK T   LD + ++W+E HR QRK         DF+DVM+S+
Sbjct: 245 SDAFPTLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSL 304

Query: 315 IKDDDEQLSGYVGDSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQ 374
            +        Y  ++ IK+ CLA+IL GSDT+ +T+TW +SLLLNN+E LKKAQ E++  
Sbjct: 305 AEQGKLSHLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIH 364

Query: 375 VGRQKQVTESDVKNLIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVN 434
           VGR + V +SD++NL+YLQAI+KE+LRLYPA P+  P E+ EDC++ GY++P  TRLIVN
Sbjct: 365 VGRDRNVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVN 424

Query: 435 IQKLQRDPLVWEEPNEFRPERFLTTH-KDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIH 494
           + K+QRDP V+ EPNEFRPERF+T   K+FDVRGQN +L+PFG+GRR CPG+S A Q++H
Sbjct: 425 VWKIQRDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLH 484

Query: 495 LTLANLLHGFEIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPRL 524
           L LA  LH F++       +DM E+ GLT  K +PLEV+++PR+
Sbjct: 485 LGLARFLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRI 518

BLAST of CSPI03G42320 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 457.6 bits (1176), Expect = 1.4e-128
Identity = 233/514 (45.33%), Postives = 339/514 (65.95%), Query Frame = 0

Query: 24  IFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWPVIGHLHLLSSSEP-GHKTLGKMA 83
           +F L+    +    +LF   ++  +++  P   GAWP+IGHLHLLS  E   ++TLGKMA
Sbjct: 5   LFSLFVPILVFVFIALFKKSKKPKHVK-APAPSGAWPIIGHLHLLSGKEQLLYRTLGKMA 64

Query: 84  DSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFASRPKLTSAKLLGYNNSMFGLAQY 143
           D  GP  +L+LG     VVS++E+A++C T ND   ASRP   +AK +GY+ ++FG A Y
Sbjct: 65  DQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPY 124

Query: 144 GPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINKLYQLCTK----EKPLVEMKAWF 203
             FWR MRK+ +LELLS  RLQ  + +R+SEI   +  LY L  K    E  +V++K+W 
Sbjct: 125 SAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVMVDLKSWL 184

Query: 204 EDITLNIMFKIIFGKRF-------TDDLKGDQDHRKTFRDLMELFGVFVPSDSLPFLSWL 263
           ED++LN+M +++ GKR+        +D +  +  RK   +   L G+F  SD+ P L W 
Sbjct: 185 EDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWF 244

Query: 264 DLGGYEKAMKTTSKVLDEVFDKWLEEHR-QRKIENNDNGAEDFMDVMLSIIKDDDEQLSG 323
           D  G+EK MK T + LD + ++W+E HR QRK+    +   DF+DVMLS+ +        
Sbjct: 245 DFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKFSHLQ 304

Query: 324 YVGDSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQVGRQKQVTES 383
           +   + IK+ CLA+IL GS+T+ +T+TW +SLLLNN++ LKKAQ E++  VGR + V +S
Sbjct: 305 HDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDS 364

Query: 384 DVKNLIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVNIQKLQRDPLV 443
           D++NL+Y+QAI+KE+LRLYPA P+    E+ EDC++ GY++   TR++VN+ K+QRDP V
Sbjct: 365 DIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRV 424

Query: 444 WEEPNEFRPERFLTTH-KDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIHLTLANLLHGF 503
           + EPNEFRPERF+T   K+FDVRGQN +L+PFG+GRR CPG+S A Q++HL LA  L  F
Sbjct: 425 YMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSF 484

Query: 504 EIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPRL 524
           ++       +DM ES GLT  K +PLE++++PRL
Sbjct: 485 DVKTVMDMPVDMTESPGLTIPKATPLEILISPRL 517

BLAST of CSPI03G42320 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 436.0 bits (1120), Expect = 4.2e-122
Identity = 235/524 (44.85%), Postives = 324/524 (61.83%), Query Frame = 0

Query: 15  MISMSLFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWPVIGHLHLLSSSEP- 74
           + S+ + IL+F    LF           + +K      P   GAWP+IGHLHLL   E  
Sbjct: 5   LFSLFVSILVFVFIALF----------KKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQL 64

Query: 75  GHKTLGKMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFASRPKLTSAKLLGYN 134
            ++TLGKMAD  GP  +L+LG     V S++E+A++C T ND   AS     +AK +GY 
Sbjct: 65  LYRTLGKMADHYGPAMSLRLGSSETFVGSSFEVAKDCFTVNDKALASL-MTAAAKHMGY- 124

Query: 135 NSMFGLAQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINKLYQLCTK----EK 194
                      FW  MRK+  +ELLS  RLQ    +R+SEI   +  LY L  K    E 
Sbjct: 125 ----------VFWLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSEP 184

Query: 195 PLVEMKAWFEDITLNIMFKIIFGKRF--------TDDLKGDQDHRKTFRDLMELFGVFVP 254
            +V++K+W ED+  N++ +++ GKR+        ++  +  +  RK       L G+F  
Sbjct: 185 VMVDLKSWLEDMIANMIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTV 244

Query: 255 SDSLPFLSWLDLGGYEKAMKTTSKVLDEVFDKWLEEHR-QRKIENNDNGAEDFMDVMLSI 314
           SD+ P L WLDL G+EK MK T + LD + ++W+E HR QRK+    +   DF+DVMLS+
Sbjct: 245 SDAFPKLGWLDLQGHEKEMKQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSL 304

Query: 315 IKDDDEQLSGYVGDSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQ 374
            +        Y  ++ IK  CLA+IL GS+T+ +T+TW +SLLLNN++ LKK Q E++  
Sbjct: 305 AEQGKLSHLQYDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIH 364

Query: 375 VGRQKQVTESDVKNLIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVN 434
           VGR + V +SD+KNL+YLQAI+KE+LRLYPA P+    E+ EDC++ GY++P  TRLIVN
Sbjct: 365 VGRDRNVEDSDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVN 424

Query: 435 IQKLQRDPLVWEEPNEFRPERFLTTH-KDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIH 494
           + K+QRDP V+ EPNEFRPERF+T   KDFDVRGQN +L+PFG+GRR CPG S A Q++H
Sbjct: 425 VWKIQRDPKVYMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLH 484

Query: 495 LTLANLLHGFEIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPRL 524
           L LA  LH FE+       +DM ES GLT TK +PLEV++ PRL
Sbjct: 485 LGLARFLHSFEVKTVLDRPVDMSESPGLTITKATPLEVLINPRL 506

BLAST of CSPI03G42320 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 363.6 bits (932), Expect = 2.7e-100
Identity = 201/515 (39.03%), Postives = 305/515 (59.22%), Query Frame = 0

Query: 22  ILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWPVIGHLHLLSSSEPGHKTLGKM 81
           I++F L  LF    L  + +  R KN     P+  GAWP++GHLHL  +  P H T G M
Sbjct: 4   IMLFLLSALFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGAM 63

Query: 82  ADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFASRPKLTSAKLLGYNNSMFGLAQ 141
           AD  GP+F  KLG  + +++++ E+A+E  T +D +   RP+LT++KLLGYN+S    + 
Sbjct: 64  ADVYGPVFMAKLGSIKVMIINSKEVAKEIYTVHDKLL-ERPELTASKLLGYNDSFLTFSP 123

Query: 142 YGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINKLY-----QLCTKEKPLVEMKA 201
           YG +WR +RK+   EL ST  +      R  E   +   LY     +   KE  LV+MK 
Sbjct: 124 YGLYWREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVDMKQ 183

Query: 202 WFEDITLNIMFKIIFGKRF-----TDDLKGDQDHRKTFRDLMELFGVFVPSDSLPFLSWL 261
            F D+T NI   ++ GKR+       ++K  +   K  R+ ++ F +F+ SD  P L +L
Sbjct: 184 EFIDLTANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGFL 243

Query: 262 DLGGYEKAMKTTSKVLDEVFDKWLEEHRQRKIENNDNGAEDFMDVMLSIIKDDDEQLSGY 321
           D    ++ MK T+K LD+V + W+EEH+ ++  ++     D++D+++ I+  D  ++ G 
Sbjct: 244 D-WKTKRGMKRTAKGLDKVAEGWIEEHKNKR-SDHGRSENDYLDILIKILGQD--KIPGL 303

Query: 322 VG-DSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQVGRQKQVTES 381
               + IKA CL ++LAGS+T    + W +SLLLNN   L+KAQ EL+ ++G+++ V E 
Sbjct: 304 SDTHTKIKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEEL 363

Query: 382 DVKNLIYLQAIVKESLRLYPALPISIPHESTEDCSI--FGYHIPSRTRLIVNIQKLQRDP 441
           D+K+L+YLQAIVKE+ RLYP +P+       ED  I     H+P+ T+L+V+  K+ RDP
Sbjct: 364 DIKDLVYLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDP 423

Query: 442 LVWEEPNEFRPERFLTTHKDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIHLTLANLLHG 501
            VW  P +F PERFLT++++ DV GQ+ +  PFG GRR CP      +++H  L   LH 
Sbjct: 424 NVWSNPEQFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHS 483

Query: 502 FEIDRPSKDLLDMEESVGLTSTKKSPLEVVLTPRL 524
           F++ RPS   +DM ES GL + K +PLEV + PRL
Sbjct: 484 FDLARPSSQDVDMTESNGLVNHKATPLEVNIIPRL 513

BLAST of CSPI03G42320 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 362.5 bits (929), Expect = 5.9e-100
Identity = 200/510 (39.22%), Postives = 301/510 (59.02%), Query Frame = 0

Query: 16  ISMSLFILIFFLYTLFWLLNLTSLFTSQRRKNNLQPLPVARGAWPVIGHLHLLSSSEPGH 75
           + +SLF L   ++   +L    S     R + +   +P   GA P+ GHLHLL   +   
Sbjct: 8   LQLSLFSLALVIFGYIFLRKQLS-----RCEVDSSTIPEPLGALPLFGHLHLLRGKKLLC 67

Query: 76  KTLGKMADSNGPIFTLKLGIHRAIVVSNWEIAQECLTTNDIIFASRPKLTSAKLLGYNNS 135
           K L  M+  +GPIF+LKLG +R +V S+ +  ++C TTND+  A+RP +   + +GYNN+
Sbjct: 68  KKLAAMSQKHGPIFSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNA 127

Query: 136 MFGLAQYGPFWRHMRKVVSLELLSTHRLQQFQPIRISEIQSSINKLYQLCTKEKPLVEMK 195
              LA YG +WR +RK+V++ L S H ++    IR SE+ + I  LY+       +V++ 
Sbjct: 128 SLTLAPYGDYWRELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLYK-GNGGTSIVKID 187

Query: 196 AWFEDITLNIMFKIIFGKR--FTDDLKGDQDHRKTFRDLMELFGVFVPSDSLPFLSWLDL 255
             FE +T NI+ + + GKR  F +    +  +++  +    L  + +  D +P+L WLD 
Sbjct: 188 MLFEFLTFNIILRKMVGKRIGFGEVNSDEWRYKEALKHCEYLAVIPMIGDVIPWLGWLDF 247

Query: 256 GGYEKAMKTTSKVLDEVFDKWLEEHRQRKIENNDNGAEDFMDVMLSIIKDDDEQLSGYVG 315
                 MK   K LD V  KWL EH +++  N  +     MD++L I+  +D  +SG+V 
Sbjct: 248 -AKNSQMKRLFKELDSVNTKWLHEHLKKRSRNEKDQERTIMDLLLDIL-PEDIVISGHVR 307

Query: 316 DSVIKANCLAMILAGSDTTTTTMTWTLSLLLNNQETLKKAQIELEEQVGRQKQVTESDVK 375
           D ++KA  LA+ L GSD+T+ T+TW +SLLLNN   L+ AQ E++  VG+ + + ESD++
Sbjct: 308 DVIVKATILALTLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVGKGRWIEESDIQ 367

Query: 376 NLIYLQAIVKESLRLYPALPISIPHESTEDCSIFGYHIPSRTRLIVNIQKLQRDPLVWEE 435
           NL YLQAIVKE+ RLYP  P++   E+ EDC + GY +   TRL+VNI KL RDP +W +
Sbjct: 368 NLKYLQAIVKETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWKLHRDPKIWPD 427

Query: 436 PNEFRPERFLTTHKDFDVRGQNPQLIPFGNGRRMCPGTSFAFQIIHLTLANLLHGFEIDR 495
           P  F+PERF+      +    N + IPFG+GRR CPG +   +++H  LA LL GFE+ +
Sbjct: 428 PKTFKPERFMEDKSQCE--KSNFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFELHK 487

Query: 496 PSKDLLDMEESVGLTSTKKSPLEVVLTPRL 524
            S + LDM E  GL   K +P+EVV+ PRL
Sbjct: 488 VSDEPLDMAEGPGLALPKINPVEVVVMPRL 507