CSPI03G41950 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G41950
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	ADP-ribosylation factor
Location	Chr3: 36034549 .. 36034904 (-)
RNA-Seq Expression	CSPI03G41950
Synteny	CSPI03G41950

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAGAAGAGCCATGGCAGACGGGGGACTACCATCATCTGTACTTTGCTAAAAGCAATCAGATTTGTCAAACATTTCTGTACAGCTTTATACTACTACAAAGGGTCAAATGCTGCACTAGCATCTCAATCAAATTTGTCCATGAAGGGTTCATTTTTCTTGCTCTCTCCATCCCCTTCAATACACCTTTGTGCAGCTCTCAGAAAAAAGAAGGAACATCCGAGTTACGAACAGAGATGGGGGCTCACCTTTGCAAAGCTCTTCAATCGGTTGTTTACTAAACCTGAGATGAGAATTCTGATGGTTGGTCTTGATGCCGCTGGTAAGACGACGACCATTTTCTATAAACTCTAG

mRNA sequence

ATGGCGAGAAGAGCCATGGCAGACGGGGGACTACCATCATCTGGTTCATTTTTCTTGCTCTCTCCATCCCCTTCAATACACCTTTGTGCAGCTCTCAGAAAAAAGAAGGAACATCCGAGTTACGAACAGAGATGGGGGCTCACCTTTGCAAAGCTCTTCAATCGGTTGTTTACTAAACCTGAGATGAGAATTCTGATGGTTGGTCTTGATGCCGCTGGTAAGACGACGACCATTTTCTATAAACTCTAG

Coding sequence (CDS)

ATGGCGAGAAGAGCCATGGCAGACGGGGGACTACCATCATCTGGTTCATTTTTCTTGCTCTCTCCATCCCCTTCAATACACCTTTGTGCAGCTCTCAGAAAAAAGAAGGAACATCCGAGTTACGAACAGAGATGGGGGCTCACCTTTGCAAAGCTCTTCAATCGGTTGTTTACTAAACCTGAGATGAGAATTCTGATGGTTGGTCTTGATGCCGCTGGTAAGACGACGACCATTTTCTATAAACTCTAG

Protein sequence

MARRAMADGGLPSSGSFFLLSPSPSIHLCAALRKKKEHPSYEQRWGLTFAKLFNRLFTKPEMRILMVGLDAAGKTTTIFYKL*

Homology

[Close]

BLAST of CSPI03G41950 vs. ExPASy Swiss-Prot
Match: Q06396 (ADP-ribosylation factor 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0813400 PE=2 SV=3)

HSP 1 Score: 60.5 bits (145), Expect = 1.1e-08
Identity = 31/37 (83.78%), Postives = 32/37 (86.49%), Query Frame = 0


Query: 46 GLTFAKLFNRLFTKPEMRILMVGLDAAGKTTTIFYKL 83
          GLTF KLF+RLF K EMRILMVGLDAAGK TTI YKL
Sbjct: 2  GLTFTKLFSRLFAKKEMRILMVGLDAAGK-TTILYKL 37

[Close]

BLAST of CSPI03G41950 vs. ExPASy Swiss-Prot
Match: Q9LQC8 (ADP-ribosylation factor 2-A OS=Arabidopsis thaliana OX=3702 GN=ARF2-A PE=2 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 1.1e-08
Identity = 31/37 (83.78%), Postives = 33/37 (89.19%), Query Frame = 0


Query: 46 GLTFAKLFNRLFTKPEMRILMVGLDAAGKTTTIFYKL 83
          GL+FAKLF+RLF K EMRILMVGLDAAGK TTI YKL
Sbjct: 2  GLSFAKLFSRLFAKKEMRILMVGLDAAGK-TTILYKL 37

[Close]

BLAST of CSPI03G41950 vs. ExPASy Swiss-Prot
Match: P0DH91 (ADP-ribosylation factor 2-B OS=Arabidopsis thaliana OX=3702 GN=ARF2-B PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.1e-08
Identity = 31/37 (83.78%), Postives = 33/37 (89.19%), Query Frame = 0


Query: 46 GLTFAKLFNRLFTKPEMRILMVGLDAAGKTTTIFYKL 83
          GL+FAKLF+RLF K EMRILMVGLDAAGK TTI YKL
Sbjct: 2  GLSFAKLFSRLFAKKEMRILMVGLDAAGK-TTILYKL 37

[Close]

BLAST of CSPI03G41950 vs. ExPASy Swiss-Prot
Match: P51823 (ADP-ribosylation factor 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ARF PE=2 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 1.1e-08
Identity = 31/37 (83.78%), Postives = 32/37 (86.49%), Query Frame = 0


Query: 46 GLTFAKLFNRLFTKPEMRILMVGLDAAGKTTTIFYKL 83
          GLTF KLF+RLF K EMRILMVGLDAAGK TTI YKL
Sbjct: 2  GLTFTKLFSRLFAKKEMRILMVGLDAAGK-TTILYKL 37

[Close]

BLAST of CSPI03G41950 vs. ExPASy Swiss-Prot
Match: P49076 (ADP-ribosylation factor OS=Zea mays OX=4577 GN=ARF1 PE=2 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 1.1e-08
Identity = 31/37 (83.78%), Postives = 32/37 (86.49%), Query Frame = 0


Query: 46 GLTFAKLFNRLFTKPEMRILMVGLDAAGKTTTIFYKL 83
          GLTF KLF+RLF K EMRILMVGLDAAGK TTI YKL
Sbjct: 2  GLTFTKLFSRLFAKKEMRILMVGLDAAGK-TTILYKL 37

[Close]

BLAST of CSPI03G41950 vs. ExPASy TrEMBL
Match: A0A0A0LGD5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G851775 PE=4 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 3.5e-31
Identity = 68/68 (100.00%), Postives = 68/68 (100.00%), Query Frame = 0


Query: 15 GSFFLLSPSPSIHLCAALRKKKEHPSYEQRWGLTFAKLFNRLFTKPEMRILMVGLDAAGK 74
          GSFFLLSPSPSIHLCAALRKKKEHPSYEQRWGLTFAKLFNRLFTKPEMRILMVGLDAAGK
Sbjct: 3  GSFFLLSPSPSIHLCAALRKKKEHPSYEQRWGLTFAKLFNRLFTKPEMRILMVGLDAAGK 62

Query: 75 TTTIFYKL 83
          TTTIFYKL
Sbjct: 63 TTTIFYKL 70

[Close]

BLAST of CSPI03G41950 vs. ExPASy TrEMBL
Match: A0A371E2G0 (ADP-ribosylation factor 2 (Fragment) OS=Mucuna pruriens OX=157652 GN=ARF PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.9e-09
Identity = 44/71 (61.97%), Postives = 46/71 (64.79%), Query Frame = 0


Query: 16 SFFLLSPSPSIHLCAALRKKKEH----PSYEQRWGLTFAKLFNRLFTKPEMRILMVGLDA 75
          SF LLS S S+ L A  R    H    P  E   GLTF KLF+RLF K EMRILMVGLDA
Sbjct: 28 SFALLSLSLSLSLTALSRTPNFHLQRDPPAEGEMGLTFTKLFSRLFAKKEMRILMVGLDA 87

Query: 76 AGKTTTIFYKL 83
          AGK TTI YKL
Sbjct: 88 AGK-TTILYKL 97

[Close]

BLAST of CSPI03G41950 vs. ExPASy TrEMBL
Match: A0A426YMP4 (Uncharacterized protein OS=Ensete ventricosum OX=4639 GN=B296_00050132 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 5.5e-08
Identity = 39/57 (68.42%), Postives = 41/57 (71.93%), Query Frame = 0


Query: 30  AALRKKKEHPSYE----QRWGLTFAKLFNRLFTKPEMRILMVGLDAAGKTTTIFYKL 83
           A  RKKK   S +     R GLTFAKLF+RLF K EMRILMVGLDAAGK TTI YKL
Sbjct: 46  AKRRKKKSRASLQIYRSGRMGLTFAKLFSRLFAKKEMRILMVGLDAAGK-TTILYKL 101

[Close]

BLAST of CSPI03G41950 vs. ExPASy TrEMBL
Match: A0A182BFF6 (Putative adp-ribosylation factor 2 OS=Lupinus angustifolius OX=3871 PE=4 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 7.2e-08
Identity = 37/58 (63.79%), Postives = 41/58 (70.69%), Query Frame = 0


Query: 25 SIHLCAALRKKKEHPSYEQRWGLTFAKLFNRLFTKPEMRILMVGLDAAGKTTTIFYKL 83
          S  + AA R+ +E    E   GLTF KLF+RLF K EMRILMVGLDAAGK TTI YKL
Sbjct: 4  STRMQAAAREIEERLEGEGEMGLTFTKLFSRLFAKKEMRILMVGLDAAGK-TTILYKL 60

[Close]

BLAST of CSPI03G41950 vs. ExPASy TrEMBL
Match: A0A6A5LQX7 (Uncharacterized protein OS=Lupinus albus OX=3870 GN=Lal_00026390 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.7e-07
Identity = 34/46 (73.91%), Postives = 35/46 (76.09%), Query Frame = 0


Query: 37 EHPSYEQRWGLTFAKLFNRLFTKPEMRILMVGLDAAGKTTTIFYKL 83
          E P  E   GLTF KLF+RLF K EMRILMVGLDAAGK TTI YKL
Sbjct: 6  ESPQGEGEMGLTFTKLFSRLFAKKEMRILMVGLDAAGK-TTILYKL 50

[Close]

BLAST of CSPI03G41950 vs. NCBI nr
Match: XP_038885435.1 (ADP-ribosylation factor 2-like [Benincasa hispida])

HSP 1 Score: 82.8 bits (203), Expect = 1.5e-12
Identity = 48/70 (68.57%), Postives = 50/70 (71.43%), Query Frame = 0


Query: 13  SSGSFFLLSPSPSIHLCAALRKKKEHPSYEQRWGLTFAKLFNRLFTKPEMRILMVGLDAA 72
           S   FFLLS S SIHLCAALR   +        GLTFAKLF+RLF K EMRILMVGLDAA
Sbjct: 44  SEARFFLLSSSLSIHLCAALRGTSK---LRAEMGLTFAKLFSRLFAKKEMRILMVGLDAA 103

Query: 73  GKTTTIFYKL 83
           GK TTI YKL
Sbjct: 104 GK-TTILYKL 109

[Close]

BLAST of CSPI03G41950 vs. NCBI nr
Match: KAG6598215.1 (hypothetical protein SDJN03_07993, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 72.8 bits (177), Expect = 1.6e-09
Identity = 46/73 (63.01%), Postives = 48/73 (65.75%), Query Frame = 0


Query: 13  SSGSFFLLSPSPSIH---LCAALRKKKEHPSYEQRWGLTFAKLFNRLFTKPEMRILMVGL 72
           S   FFLLSPS SIH   LC AL       +     GLTF KLF+RLF K EMRILMVGL
Sbjct: 818 SEARFFLLSPSLSIHLWPLCVALGGTL---TLRTDMGLTFGKLFSRLFAKKEMRILMVGL 877

Query: 73  DAAGKTTTIFYKL 83
           DAAGK TTI YKL
Sbjct: 878 DAAGK-TTILYKL 886

[Close]

BLAST of CSPI03G41950 vs. NCBI nr
Match: RDX60239.1 (ADP-ribosylation factor 2, partial [Mucuna pruriens])

HSP 1 Score: 70.9 bits (172), Expect = 6.0e-09
Identity = 44/71 (61.97%), Postives = 46/71 (64.79%), Query Frame = 0


Query: 16 SFFLLSPSPSIHLCAALRKKKEH----PSYEQRWGLTFAKLFNRLFTKPEMRILMVGLDA 75
          SF LLS S S+ L A  R    H    P  E   GLTF KLF+RLF K EMRILMVGLDA
Sbjct: 28 SFALLSLSLSLSLTALSRTPNFHLQRDPPAEGEMGLTFTKLFSRLFAKKEMRILMVGLDA 87

Query: 76 AGKTTTIFYKL 83
          AGK TTI YKL
Sbjct: 88 AGK-TTILYKL 97

[Close]

BLAST of CSPI03G41950 vs. NCBI nr
Match: RRT52988.1 (hypothetical protein B296_00050132 [Ensete ventricosum])

HSP 1 Score: 66.6 bits (161), Expect = 1.1e-07
Identity = 39/57 (68.42%), Postives = 41/57 (71.93%), Query Frame = 0


Query: 30  AALRKKKEHPSYE----QRWGLTFAKLFNRLFTKPEMRILMVGLDAAGKTTTIFYKL 83
           A  RKKK   S +     R GLTFAKLF+RLF K EMRILMVGLDAAGK TTI YKL
Sbjct: 46  AKRRKKKSRASLQIYRSGRMGLTFAKLFSRLFAKKEMRILMVGLDAAGK-TTILYKL 101

[Close]

BLAST of CSPI03G41950 vs. NCBI nr
Match: CAB3461131.1 (unnamed protein product [Digitaria exilis])

HSP 1 Score: 66.2 bits (160), Expect = 1.5e-07
Identity = 40/71 (56.34%), Postives = 45/71 (63.38%), Query Frame = 0


Query: 12 PSSGSFFLLSPSPSIHLCAALRKKKEHPSYEQRWGLTFAKLFNRLFTKPEMRILMVGLDA 71
          P+  +   +S SP I L   LR +   P      GLTF KLF+RLF K EMRILMVGLDA
Sbjct: 11 PTRNAAAFISASP-IPLPLRLRSEPAPPHAGAEMGLTFTKLFSRLFAKKEMRILMVGLDA 70

Query: 72 AGKTTTIFYKL 83
          AGK TTI YKL
Sbjct: 71 AGK-TTILYKL 79

[Close]

BLAST of CSPI03G41950 vs. TAIR 10
Match: AT1G70490.1 (Ras-related small GTP-binding family protein )

HSP 1 Score: 60.5 bits (145), Expect = 7.6e-10
Identity = 31/37 (83.78%), Postives = 33/37 (89.19%), Query Frame = 0


Query: 46 GLTFAKLFNRLFTKPEMRILMVGLDAAGKTTTIFYKL 83
          GL+FAKLF+RLF K EMRILMVGLDAAGK TTI YKL
Sbjct: 2  GLSFAKLFSRLFAKKEMRILMVGLDAAGK-TTILYKL 37

[Close]

BLAST of CSPI03G41950 vs. TAIR 10
Match: AT1G23490.1 (ADP-ribosylation factor 1 )

HSP 1 Score: 60.5 bits (145), Expect = 7.6e-10
Identity = 31/37 (83.78%), Postives = 33/37 (89.19%), Query Frame = 0


Query: 46 GLTFAKLFNRLFTKPEMRILMVGLDAAGKTTTIFYKL 83
          GL+FAKLF+RLF K EMRILMVGLDAAGK TTI YKL
Sbjct: 2  GLSFAKLFSRLFAKKEMRILMVGLDAAGK-TTILYKL 37

[Close]

BLAST of CSPI03G41950 vs. TAIR 10
Match: AT1G10630.1 (ADP-ribosylation factor A1F )

HSP 1 Score: 60.5 bits (145), Expect = 7.6e-10
Identity = 31/37 (83.78%), Postives = 33/37 (89.19%), Query Frame = 0


Query: 46 GLTFAKLFNRLFTKPEMRILMVGLDAAGKTTTIFYKL 83
          GL+FAKLF+RLF K EMRILMVGLDAAGK TTI YKL
Sbjct: 2  GLSFAKLFSRLFAKKEMRILMVGLDAAGK-TTILYKL 37

[Close]

BLAST of CSPI03G41950 vs. TAIR 10
Match: AT1G70490.3 (Ras-related small GTP-binding family protein )

HSP 1 Score: 60.5 bits (145), Expect = 7.6e-10
Identity = 31/37 (83.78%), Postives = 33/37 (89.19%), Query Frame = 0


Query: 46 GLTFAKLFNRLFTKPEMRILMVGLDAAGKTTTIFYKL 83
          GL+FAKLF+RLF K EMRILMVGLDAAGK TTI YKL
Sbjct: 2  GLSFAKLFSRLFAKKEMRILMVGLDAAGK-TTILYKL 37

[Close]

BLAST of CSPI03G41950 vs. TAIR 10
Match: AT1G70490.2 (Ras-related small GTP-binding family protein )

HSP 1 Score: 60.5 bits (145), Expect = 7.6e-10
Identity = 31/37 (83.78%), Postives = 33/37 (89.19%), Query Frame = 0


Query: 46 GLTFAKLFNRLFTKPEMRILMVGLDAAGKTTTIFYKL 83
          GL+FAKLF+RLF K EMRILMVGLDAAGK TTI YKL
Sbjct: 2  GLSFAKLFSRLFAKKEMRILMVGLDAAGK-TTILYKL 37

The following BLAST results are available for this feature:

BLAST of CSPI03G41950 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q06396	1.1e-08	83.78	ADP-ribosylation factor 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g08134...	[more]
Q9LQC8	1.1e-08	83.78	ADP-ribosylation factor 2-A OS=Arabidopsis thaliana OX=3702 GN=ARF2-A PE=2 SV=2	[more]
P0DH91	1.1e-08	83.78	ADP-ribosylation factor 2-B OS=Arabidopsis thaliana OX=3702 GN=ARF2-B PE=2 SV=1	[more]
P51823	1.1e-08	83.78	ADP-ribosylation factor 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ARF PE=2 S...	[more]
P49076	1.1e-08	83.78	ADP-ribosylation factor OS=Zea mays OX=4577 GN=ARF1 PE=2 SV=2	[more]

BLAST of CSPI03G41950 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LGD5	3.5e-31	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G851775 PE=4 SV=1	[more]
A0A371E2G0	2.9e-09	61.97	ADP-ribosylation factor 2 (Fragment) OS=Mucuna pruriens OX=157652 GN=ARF PE=3 SV...	[more]
A0A426YMP4	5.5e-08	68.42	Uncharacterized protein OS=Ensete ventricosum OX=4639 GN=B296_00050132 PE=4 SV=1	[more]
A0A182BFF6	7.2e-08	63.79	Putative adp-ribosylation factor 2 OS=Lupinus angustifolius OX=3871 PE=4 SV=1	[more]
A0A6A5LQX7	2.7e-07	73.91	Uncharacterized protein OS=Lupinus albus OX=3870 GN=Lal_00026390 PE=3 SV=1	[more]

BLAST of CSPI03G41950 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038885435.1	1.5e-12	68.57	ADP-ribosylation factor 2-like [Benincasa hispida]	[more]
KAG6598215.1	1.6e-09	63.01	hypothetical protein SDJN03_07993, partial [Cucurbita argyrosperma subsp. sorori...	[more]
RDX60239.1	6.0e-09	61.97	ADP-ribosylation factor 2, partial [Mucuna pruriens]	[more]
RRT52988.1	1.1e-07	68.42	hypothetical protein B296_00050132 [Ensete ventricosum]	[more]
CAB3461131.1	1.5e-07	56.34	unnamed protein product [Digitaria exilis]	[more]

BLAST of CSPI03G41950 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G70490.1	7.6e-10	83.78	Ras-related small GTP-binding family protein	[more]
AT1G23490.1	7.6e-10	83.78	ADP-ribosylation factor 1	[more]
AT1G10630.1	7.6e-10	83.78	ADP-ribosylation factor A1F	[more]
AT1G70490.3	7.6e-10	83.78	Ras-related small GTP-binding family protein	[more]
AT1G70490.2	7.6e-10	83.78	Ras-related small GTP-binding family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006689	Small GTPase superfamily, ARF/SAR type	PFAM	PF00025	Arf	coord: 50..82 e-value: 1.8E-6 score: 27.6
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 41..82 e-value: 5.2E-6 score: 27.7
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 52..79

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G41950.1	CSPI03G41950.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003924	GTPase activity
molecular_function	GO:0005525	GTP binding