CSPI03G41840 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G41840
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	tRNA (guanine(37)-N1)-methyltransferase
Location	Chr3: 35924088 .. 35928008 (-)
RNA-Seq Expression	CSPI03G41840
Synteny	CSPI03G41840

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGATTTGGGCAGCCAAAGTGTAAGAAAGGGAAGAAAAAACAAAACAAAACCTTTAAACCCTTTTGAACGGTTGGTTTATCACTTTACCTTCGGCCGTTCAACTTTCCGGCAGCCGCCATCAAACCTGATTGTCGCCGTTCGAGTCGTTCGCCAATGAGATTATGGTTACAAAGCTCTTCCTGAGGCCACAGTCGCTTCCTCTCACTGCCTTTTCCCCGAAATTCATCTTCCCCCATAAGAATTCTCTCCCGATATGCTTCACCTCACCGTTTTCCTCCACCACAGTCCACTCCCAATCTCAATTCTCTCACCAAAGCCTCTCCTATGGACCTTCTCTTTGCAAGGGTATCCATCCATTTCCCTCAGTTTCCAGACAGTCTCCAATCGATCATTCTCCACCTCTTCTCAAGACCCTAACCGGAAATCTGGAGAGACAGCAGTTGCAAAACCAGGAAAGTGAAGGAGCTATATTGGACGAAGATGCCTTCACCCGGATTTTCGACGTTGCTGCACTTCGAGTTCCTTCCAGAGGCTGTTTCGAGCTTGAGAATCGACTGCGTGGCCATCTCTTGAACTGGCCGCGGATTCGCAACATTGCTAGAGTTCCGGGGGATGAGGTTGAAGCCGAAATGTTGGAGCTTGTAGGAGAGCAGAATTGGAGCAATTCCGATGATGGAGATGGAACTAGTATTGATTTGTTGCACCGAAGAATTTATGGGAAAGCTGAGGGTGATGGGGAGCCTCTGAGTCCCGTGCTGTACAGGGAAAAGCTAGTGAAAACGTTCGATTCAAAAGGATATGTGAACTTCAGACATTTGGCGAAAATGTCAAGGCTGAAGAGAAGGAAGGAGAAGAAGGAAGAAGGGAGAGATGAAAAGATGAGAAGAATGGGGAAAAGTGAATTTGCCATTGTGGAGGTTGTGGAGAGTGAGGAACTGGAAATGGATGATATGAAGGGATTGTTGGGAGATGGGTTTAAGGGTGGAAAATGGAGGGGTTCGACTAGATTGTTGCTTTTGGATGAACAATATGCAGCCAAAGGCGTAGAAGAGCTTCCAGAAGCGATTAAGGTAGCTGTTATAGTTACTTTTTCAACAATTTGATATAAGTATCTGTACTTCATTCGCGTTTCTTGATTTAATGTTTCTTGTACATCTCTCTCATTAGCGTGAAGTTGAGGAGAGCCTTGTCTTGTGTCTCACTTGTATTTTCTCTTGGTTTCTTCTACTGCAGGCTCTGCTGAAAGGAGATACCCAAGAAAGGATGAAACCGACAATTGAGCTTGTGAGGTGCAGACTTACTTTATTTTATGATTATTGGCAGATGAATGAGGTATTCACAAAATGGGAGAATTTAGGTGTGTTTGTTATTTGTTTATTTTTAATCTAATTCATTCCATCATTTCAATTTTCAATTTTGATCAATTGCTTTTTATCATTGAGGAGATACTACGATAGTCTCTAATTAATCTTCGAAGGGAACCAAATTAATTGATTTTGTTAAGGTCTCTCTCGGTTATGAGATTTAGTAATGCCTTTTTCAGTTTTCCATGTTGTATCAATTCATTTTGATAAAAGAATTATAGGTTCCAGAAAAATTTGGTGTTCAATCATTTCTTTGCATGGTTGATTAGTTAAGATGTTCGGATGTACATAAATTGGGGCATCTGGTTTTTTCTGAAGCTAAGAAGTGGAAATGCTACATCTACTTGACTATATTTTATCTTCTAAGAGATTGAATAGAGGCATGCTATTGCACACACACACGATTCACTGCATAATTGATATATTATTAATGACGATGATGTCAATTTGAGTATAACTCATTTTGTTTTTGCATAACCAAGAGTTTAGATATTCGAATGTTCCCAACTCTATATATTGTTGAACTAAAAAAACATCAATAATGTTGATCGATTTCAACACAAAACTTGGAAACATCTCATTTATTGAAACAAAAGAACCTTTAGAATTGCATATTCCCTGTCGGCCCCTAAACTACTATACACATTTCCCTTGTCAGATAAAACTTTACTATTCTGGCCAAATGATATAAGTATATTGTTCGTCTCTGTGTGATGAATGTAAGAATGCACAAATTGGCTGTTGAAATTCACTATCGAATGTCCCTTTGCACTACTTTCAGGTTTTGCAGGCTTTGCTTCCAAAGGATATGATTATTCCTTCAGCGTTTGAAACCGTTGGACACATTGCACACCTAAACCTCAGAGATGAACATCTCTCATACAAAAAGCTTATAGCGAAGGTAAATTAGGGCATGTCTTCTATCAAATATAATGGAATTCAATCGTGCTCTCATAAGAAAGCAGGCTTTCCATTCAGGTTATTTTGGATAAAAATAAGCCAAAGATACAAACAGTAGTGAATAAGAGTGATGCCATCAGCAATGATTACAGGACAATGCAACTTGAGGTTTTAGCTGGGAACCACTCCCTTGTGACCACAGTAGTTGAGAATGGTTTACGATTTCATTTGGACTTGGCAACAGTGTAAATTCTCCTCTCCTCAAAACTAGTGCAAAACTAATTGATATTTCTTCCCTTTTATCCAACTGTCATAATTGTATCATGGATGGTAATCTAACATATTTTTGGGCATATTGAGAGCAATGTTGAAATGGTTAGTCATGTTAAAAAATTACTACCAAACTTGCCTTAAGTTATTCAAAATCAAATTTGATGATATGAAGTCCCATTTAAAAGTGTACAAGTTAAATGCTAAATTGATTTTGAAGGATGAAAGGGGTGTGTGGAAGTAGTTTTTAATTTCGTACATGGAAAAATTGATCTTAACCATTTCAAAATCACTCCTCCACTATCCCTTCAAAATGACTAACTTGATAATCTCTCTCCCTTGCAAGTACTGGAATTTTCTTAGAAGGATTTTTAACCTCTCCCCTTTCAGGTATTGGAATTCAAGGCTCGCAACTGAGAGACAGAGGCTACTAAGTGTCTTTACCTGCAATGATGTTATTTGTATGTTTGTCTTAGATTCTAAAGTTAACTTTATGCTTACAGTGAAATAAGATATAGGGGCAAAAATAATAATTTGACCATTTCCTTTTCCTTTCAAATGGGAATGAACAGGTGATGTCTTTGCTGGAGTTGGTCCAATATCAATATCTGCTGCAAGAATAGTAAAGAAAGTATATGCCAATGATTTGAATCCGTATGCAGTTGAGTATCTGGAAAGAAATAGTGTTGCCAATAAACTCGAGAGGAAGATTGAGGTATGAGATCTTTATAATTTGCAACTTCAATCATGCTTTCTTGTTTGAAGATAAAGGGTTGAAAGATACTCTCTTTTATTGGTTTTAGAATTAGTGGGCTTAGGCCAAATGAAAGATTAACCCTAATATTCCTTCCTTTTTTACATTAAGCTTGTTGTCTATTTATTTTTCCCTATTGTACCTTTGTTAATGTGAGAAAATAATACGAAAGTCAGATCATGGTTTTTCTCTCGGTCCTCGGATTTTCACGTAACTCAATGTAGTATTTTCTTTTACCGCTTTCAATATGGAATCAGAGTGGGTAAAGATGAAACCCTAGACACCAACATTCATGGAACCCAAACAGAAACTAACCACGAGAGAGCTGCCACCACCGCCGTCGCCATGGAAAAACTACTCCACTAGCTTCAGACGTCGTCGGGCGAGTAGTCCGGGTCCACCCTTGGAGCTAGCCACACCACCTAGTGAAGGATCTAGCTGCACGCGCCAAGCAAGTTCGCCCACGCGTCGTTGCCTACCTTCTTTATTGTTCAACCGACTTACTCTTAACCATTCTGCTCGTTTAGTCACGGAACACTTTTGGTTAGTTGTCGATCCACGCGCCACTGCTACTAAAGTCATACATCCACACGTCGCCGCTGCCCTCTTTCACGCACTGTTGCCTTCCGAC

mRNA sequence

TTTGATTTGGGCAGCCAAAGTGTAAGAAAGGGAAGAAAAAACAAAACAAAACCTTTAAACCCTTTTGAACGGTTGGTTTATCACTTTACCTTCGGCCGTTCAACTTTCCGGCAGCCGCCATCAAACCTGATTGTCGCCGTTCGAGTCGTTCGCCAATGAGATTATGGTTACAAAGCTCTTCCTGAGGCCACAGTCGCTTCCTCTCACTGCCTTTTCCCCGAAATTCATCTTCCCCCATAAGAATTCTCTCCCGATATGCTTCACCTCACCGTTTTCCTCCACCACAGTCCACTCCCAATCTCAATTCTCTCACCAAAGCCTCTCCTATGGACCTTCTCTTTGCAAGGGTATCCATCCATTTCCCTCAGTTTCCAGACAGTCTCCAATCGATCATTCTCCACCTCTTCTCAAGACCCTAACCGGAAATCTGGAGAGACAGCAGTTGCAAAACCAGGAAAGTGAAGGAGCTATATTGGACGAAGATGCCTTCACCCGGATTTTCGACGTTGCTGCACTTCGAGTTCCTTCCAGAGGCTGTTTCGAGCTTGAGAATCGACTGCGTGGCCATCTCTTGAACTGGCCGCGGATTCGCAACATTGCTAGAGTTCCGGGGGATGAGGTTGAAGCCGAAATGTTGGAGCTTGTAGGAGAGCAGAATTGGAGCAATTCCGATGATGGAGATGGAACTAGTATTGATTTGTTGCACCGAAGAATTTATGGGAAAGCTGAGGGTGATGGGGAGCCTCTGAGTCCCGTGCTGTACAGGGAAAAGCTAGTGAAAACGTTCGATTCAAAAGGATATGTGAACTTCAGACATTTGGCGAAAATGTCAAGGCTGAAGAGAAGGAAGGAGAAGAAGGAAGAAGGGAGAGATGAAAAGATGAGAAGAATGGGGAAAAGTGAATTTGCCATTGTGGAGGTTGTGGAGAGTGAGGAACTGGAAATGGATGATATGAAGGGATTGTTGGGAGATGGGTTTAAGGGTGGAAAATGGAGGGGTTCGACTAGATTGTTGCTTTTGGATGAACAATATGCAGCCAAAGGCGTAGAAGAGCTTCCAGAAGCGATTAAGGCTCTGCTGAAAGGAGATACCCAAGAAAGGATGAAACCGACAATTGAGCTTGTGAGGTGCAGACTTACTTTATTTTATGATTATTGGCAGATGAATGAGGTTTTGCAGGCTTTGCTTCCAAAGGATATGATTATTCCTTCAGCGTTTGAAACCGTTGGACACATTGCACACCTAAACCTCAGAGATGAACATCTCTCATACAAAAAGCTTATAGCGAAGGTTATTTTGGATAAAAATAAGCCAAAGATACAAACAGTAGTGAATAAGAGTGATGCCATCAGCAATGATTACAGGACAATGCAACTTGAGGTTTTAGCTGGGAACCACTCCCTTGTGACCACAGTAGTTGAGAATGGTTTACGATTTCATTTGGACTTGGCAACAGTGTATTGGAATTCAAGGCTCGCAACTGAGAGACAGAGGCTACTAAGTGTCTTTACCTGCAATGATGTTATTTGTGATGTCTTTGCTGGAGTTGGTCCAATATCAATATCTGCTGCAAGAATAGTAAAGAAAGTATATGCCAATGATTTGAATCCGTATGCAGTTGAGTATCTGGAAAGAAATAGTGTTGCCAATAAACTCGAGAGGAAGATTGAGAGTGGGTAAAGATGAAACCCTAGACACCAACATTCATGGAACCCAAACAGAAACTAACCACGAGAGAGCTGCCACCACCGCCGTCGCCATGGAAAAACTACTCCACTAGCTTCAGACGTCGTCGGGCGAGTAGTCCGGGTCCACCCTTGGAGCTAGCCACACCACCTAGTGAAGGATCTAGCTGCACGCGCCAAGCAAGTTCGCCCACGCGTCGTTGCCTACCTTCTTTATTGTTCAACCGACTTACTCTTAACCATTCTGCTCGTTTAGTCACGGAACACTTTTGGTTAGTTGTCGATCCACGCGCCACTGCTACTAAAGTCATACATCCACACGTCGCCGCTGCCCTCTTTCACGCACTGTTGCCTTCCGAC

Coding sequence (CDS)

ATGGTTACAAAGCTCTTCCTGAGGCCACAGTCGCTTCCTCTCACTGCCTTTTCCCCGAAATTCATCTTCCCCCATAAGAATTCTCTCCCGATATGCTTCACCTCACCGTTTTCCTCCACCACAGTCCACTCCCAATCTCAATTCTCTCACCAAAGCCTCTCCTATGGACCTTCTCTTTGCAAGGGTATCCATCCATTTCCCTCAGTTTCCAGACAGTCTCCAATCGATCATTCTCCACCTCTTCTCAAGACCCTAACCGGAAATCTGGAGAGACAGCAGTTGCAAAACCAGGAAAGTGAAGGAGCTATATTGGACGAAGATGCCTTCACCCGGATTTTCGACGTTGCTGCACTTCGAGTTCCTTCCAGAGGCTGTTTCGAGCTTGAGAATCGACTGCGTGGCCATCTCTTGAACTGGCCGCGGATTCGCAACATTGCTAGAGTTCCGGGGGATGAGGTTGAAGCCGAAATGTTGGAGCTTGTAGGAGAGCAGAATTGGAGCAATTCCGATGATGGAGATGGAACTAGTATTGATTTGTTGCACCGAAGAATTTATGGGAAAGCTGAGGGTGATGGGGAGCCTCTGAGTCCCGTGCTGTACAGGGAAAAGCTAGTGAAAACGTTCGATTCAAAAGGATATGTGAACTTCAGACATTTGGCGAAAATGTCAAGGCTGAAGAGAAGGAAGGAGAAGAAGGAAGAAGGGAGAGATGAAAAGATGAGAAGAATGGGGAAAAGTGAATTTGCCATTGTGGAGGTTGTGGAGAGTGAGGAACTGGAAATGGATGATATGAAGGGATTGTTGGGAGATGGGTTTAAGGGTGGAAAATGGAGGGGTTCGACTAGATTGTTGCTTTTGGATGAACAATATGCAGCCAAAGGCGTAGAAGAGCTTCCAGAAGCGATTAAGGCTCTGCTGAAAGGAGATACCCAAGAAAGGATGAAACCGACAATTGAGCTTGTGAGGTGCAGACTTACTTTATTTTATGATTATTGGCAGATGAATGAGGTTTTGCAGGCTTTGCTTCCAAAGGATATGATTATTCCTTCAGCGTTTGAAACCGTTGGACACATTGCACACCTAAACCTCAGAGATGAACATCTCTCATACAAAAAGCTTATAGCGAAGGTTATTTTGGATAAAAATAAGCCAAAGATACAAACAGTAGTGAATAAGAGTGATGCCATCAGCAATGATTACAGGACAATGCAACTTGAGGTTTTAGCTGGGAACCACTCCCTTGTGACCACAGTAGTTGAGAATGGTTTACGATTTCATTTGGACTTGGCAACAGTGTATTGGAATTCAAGGCTCGCAACTGAGAGACAGAGGCTACTAAGTGTCTTTACCTGCAATGATGTTATTTGTGATGTCTTTGCTGGAGTTGGTCCAATATCAATATCTGCTGCAAGAATAGTAAAGAAAGTATATGCCAATGATTTGAATCCGTATGCAGTTGAGTATCTGGAAAGAAATAGTGTTGCCAATAAACTCGAGAGGAAGATTGAGAGTGGGTAA

Protein sequence

MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLCKGIHPFPSVSRQSPIDHSPPLLKTLTGNLERQQLQNQESEGAILDEDAFTRIFDVAALRVPSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLLHRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKMRRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPEAIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAHLNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVENGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYANDLNPYAVEYLERNSVANKLERKIESG*

Homology

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BLAST of CSPI03G41840 vs. ExPASy Swiss-Prot
Match: F6H2F8 (tRNA (guanine(37)-N1)-methyltransferase 1 OS=Vitis vinifera OX=29760 GN=VIT_19s0014g03930 PE=3 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 3.3e-177
Identity = 328/508 (64.57%), Postives = 394/508 (77.56%), Query Frame = 0


Query: 1   MVTKLFLRPQSLPLTAFSPKFIFPH---KNSLPICFTSPFSSTTVHSQSQFSHQSLSYGP 60
           MVTKLFLRP SL  T  S   +FP       + +C  S  ++T + + +Q    +LSYGP
Sbjct: 1   MVTKLFLRPHSLSFTLLSGIHLFPKTSLSKPITLCLLSTTATTPILTLTQTLDPNLSYGP 60

Query: 61  SLCKGIHPFPSVSRQSPIDHSPPLLKTLTGNLERQQLQNQESEGAILDEDAFTRIFDVAA 120
           SL KG  P    + Q        L+    G            E  + D++AFTR+F++ A
Sbjct: 61  SLHKGTKPLNHQNHQ--------LIAATPG-----------EEECVFDKEAFTRVFNLTA 120

Query: 121 LRVPSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGT-- 180
           +RVPS+ CF LENRLRGHLLNWPRIRN+ARVPGDEVE  +++L+GE+   NS DG  +  
Sbjct: 121 IRVPSKDCFALENRLRGHLLNWPRIRNVARVPGDEVEDGLVKLLGEKR--NSSDGSESEG 180

Query: 181 SIDLLHRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEG 240
             D L+RRIYGKAEGDGE LSPVLYR+ L KTFDS+G+ NFR+LAK+SR K++K +KEE 
Sbjct: 181 DFDSLNRRIYGKAEGDGEILSPVLYRDTLAKTFDSQGFANFRNLAKLSRPKKKKRRKEEE 240

Query: 241 RDEKMRRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGV 300
           R E  +R GK+EFA+VEVVE  E E +D++GLLG+ FK  +WRGSTRLLLLDE+YA KGV
Sbjct: 241 RSEGKKRTGKNEFAMVEVVEDGE-EGEDLRGLLGEEFKRKRWRGSTRLLLLDERYADKGV 300

Query: 301 EELPEAIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETV 360
           EELPEAIKA+LK DT + M  T ELV+C+LTLFY+YWQMNE+L+ALLP+ MI+PSAFE V
Sbjct: 301 EELPEAIKAVLKEDTGQSMTSTFELVKCKLTLFYNYWQMNEILEALLPEGMIVPSAFEMV 360

Query: 361 GHIAHLNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLV 420
           GHIAHLNLRDEHL YKKLIAKV+LDKNKPKIQTVVNK+DAI NDYRTMQLEVLAGN SLV
Sbjct: 361 GHIAHLNLRDEHLPYKKLIAKVVLDKNKPKIQTVVNKTDAIHNDYRTMQLEVLAGNRSLV 420

Query: 421 TTVVENGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKK 480
           TTV+ENG+RF +DLATVYWNSRLATERQRLL+ FT NDV+CDVF+GVGPI+ISAA+ VK+
Sbjct: 421 TTVIENGMRFQVDLATVYWNSRLATERQRLLNCFTRNDVVCDVFSGVGPIAISAAKKVKR 480

Query: 481 VYANDLNPYAVEYLERNSVANKLERKIE 504
           VYANDLNPYA+EYLE NSV NKLERKI+
Sbjct: 481 VYANDLNPYAIEYLESNSVLNKLERKIK 486

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BLAST of CSPI03G41840 vs. ExPASy Swiss-Prot
Match: Q6NQ64 (tRNA (guanine(37)-N1)-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=At4g27340 PE=2 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 3.7e-152
Identity = 294/514 (57.20%), Postives = 379/514 (73.74%), Query Frame = 0


Query: 1   MVTKLFL-RPQSLP---LTAFSPKFI---FPHKNSLPIC-FTSPFSSTTVHSQSQFSHQS 60
           MV+KL L R  SLP   ++++S +FI   +P   +L  C F+S  SSTT           
Sbjct: 1   MVSKLSLFRANSLPFPVISSYSARFILKPYPKPKTLIFCVFSSNLSSTT----------- 60

Query: 61  LSYGPSLCKGIHPFPSVSRQSPIDHSPPLLKTLTGNLERQQLQNQESEGAILDEDAFTRI 120
             YGPSL KG  P     R + I       +   G     +      +  +L+ED FTR+
Sbjct: 61  FPYGPSLLKGKKPVLDDIRLASIGRDRDAHRGKIG-----EFDESIEKDVLLNEDEFTRV 120

Query: 121 FDVAALRVPSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDG 180
           F+++A+RVP++ CF LENRLRGHLLNWPRIRNIARVPGDE+E ++++L+G +     +D 
Sbjct: 121 FEISAIRVPAKDCFALENRLRGHLLNWPRIRNIARVPGDEIEEDVVKLLGRETDEEEEDE 180

Query: 181 DGTSIDLLHRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRK--E 240
           D   +D ++RRI GKAEGDGE LS VL+R+KL +TF+S GY+ FR+LAK+SR KR++  E
Sbjct: 181 DSV-VDSVNRRIRGKAEGDGERLSSVLHRDKLARTFNSTGYLKFRNLAKISRPKRKRKTE 240

Query: 241 KKEEGRDEKMRRMGKSEFAIVEVVESEELEMDDMKGLLGDGF-KGGKWRGSTRLLLLDEQ 300
           +  EG+++++    ++E A+VEVVE+   E +D +GLLG+G+   G+WRGSTRLLLLDE+
Sbjct: 241 RTREGKEKEIASR-RNEMAVVEVVETRGGE-EDFEGLLGEGYGSRGRWRGSTRLLLLDEK 300

Query: 301 YAAKGVEELPEAIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIP 360
           Y+ + V++LPEAIK L           + ELV+CRLTLFYDYW M E+L+A+LPK MI+P
Sbjct: 301 YSGEEVQDLPEAIKVLFAEAKMADASLSFELVKCRLTLFYDYWPMIEILEAVLPKGMIVP 360

Query: 361 SAFETVGHIAHLNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLA 420
           SAFE VGHIAHLNLRDEHL YK+LIAKV+LDKN+PKIQTVVNK D I ND+RTMQLEVLA
Sbjct: 361 SAFEMVGHIAHLNLRDEHLPYKRLIAKVVLDKNQPKIQTVVNKIDPIHNDFRTMQLEVLA 420

Query: 421 GNHSLVTTVVENGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISA 480
           GNHSLVT VVENGLRFH+DLA VYWNS+L TERQRLL  F  NDV+CDVFAGVGPI+++A
Sbjct: 421 GNHSLVTLVVENGLRFHVDLARVYWNSKLGTERQRLLLGFDQNDVVCDVFAGVGPIALAA 480

Query: 481 ARIVKKVYANDLNPYAVEYLERNSVANKLERKIE 504
           ARIVK+VYANDLNP+AVE++E+NSV NKLE++IE
Sbjct: 481 ARIVKRVYANDLNPHAVEFMEQNSVVNKLEKRIE 495

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BLAST of CSPI03G41840 vs. ExPASy Swiss-Prot
Match: Q93YU6 (tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3g56120 PE=2 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 5.8e-49
Identity = 104/219 (47.49%), Postives = 143/219 (65.30%), Query Frame = 0


Query: 281 TRLLLLDEQYAAKGVEELPEAIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQA 340
           TRL++L E      + E+PE     LK  ++      +E+V   +TL Y YW  + +L+ 
Sbjct: 52  TRLVILSESVKNADLSEIPEEKLNQLKKLSE------LEVVPHSVTLGYSYWSADHLLKQ 111

Query: 341 LLPKDMIIPSAFETVGHIAHLNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDY 400
           +LP  + IPS+FET+GHIAHLNL DE L +K +IAKVI DKN P+I+T+VNK   ISN++
Sbjct: 112 ILPDGLDIPSSFETIGHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEF 171

Query: 401 RTMQLEVLAGNHSLVTTVVENGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFA 460
           R  + EVLAG + + T V + G RF LD   VYWNSRL  E  RL S+F   + +CD+FA
Sbjct: 172 RVPKFEVLAGENGMETEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFA 231

Query: 461 GVGPISISAARIVKKVYANDLNPYAVEYLERNSVANKLE 500
           G+GP +I AA+    VYANDLNP +V YL+ N+  NK++
Sbjct: 232 GIGPFAIPAAQKGCFVYANDLNPDSVRYLKINAKFNKVD 264

[Close]

BLAST of CSPI03G41840 vs. ExPASy Swiss-Prot
Match: F4NUJ6 (tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strain JAM81 / FGSC 10211) OX=684364 GN=TRM5 PE=3 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.9e-48
Identity = 106/228 (46.49%), Postives = 146/228 (64.04%), Query Frame = 0


Query: 276 KWRGSTRLLLLDEQYAAKGVEELPEAIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMN 335
           KW    RLLLLD    A  + +LP  +K       +  +K   +LV+  + L YDYW  +
Sbjct: 65  KW----RLLLLDPSMEASEINDLPNPLK-------EFALKHEAKLVKHTIELKYDYWTSD 124

Query: 336 EVLQALLPKDMIIPSAFETVGHIAHLNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDA 395
           +VL+++LP +M  P AFETVGHIAHLNLRD +  +K +I +VILDK+   I+TVVNK D 
Sbjct: 125 QVLRSILPDEMETPGAFETVGHIAHLNLRDRYQPFKHIIGQVILDKSS-HIKTVVNKLDN 184

Query: 396 ISNDYRTMQLEVLAGNHSLVTTVVENGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVI 455
           I + +R  Q+E+LAG + +   + E G  FH D + VYWNSRL  E  R++ +F  ND+I
Sbjct: 185 IDHTFRFFQMEILAGINDMNAKLKEGGCFFHFDFSKVYWNSRLQGEHDRIIKLFGQNDLI 244

Query: 456 CDVFAGVGPISISAARIVK-KVYANDLNPYAVEYLERNSVANKLERKI 503
           CDVFAGVGP ++ AA+  +  V+ANDLNP + +YL  N   NKLE +I
Sbjct: 245 CDVFAGVGPFALPAAKHKRCVVFANDLNPQSFKYLMENIKLNKLETRI 280

[Close]

BLAST of CSPI03G41840 vs. ExPASy Swiss-Prot
Match: A9T6G5 (tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPADRAFT_141055 PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.4e-47
Identity = 104/229 (45.41%), Postives = 149/229 (65.07%), Query Frame = 0


Query: 280 STRLLLLDEQYAAKGVEELPE----AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMN 339
           S RLLL  E  A   +E + E    A+K ++           +++V+  + L Y YW + 
Sbjct: 60  SMRLLLFSEAIAQPDLEGVSEEKILAVKEIV----------PLDVVQHEVVLDYSYWPVE 119

Query: 340 EVLQALLPKDMIIPSAFETVGHIAHLNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDA 399
            +L+ +LP    +PS+FET+GHIAHLNLR++ L+YKK+IA+VILDKN PK++TVVNK   
Sbjct: 120 HILKEILPAGCEVPSSFETIGHIAHLNLREDLLTYKKIIAEVILDKN-PKLKTVVNKVGT 179

Query: 400 ISNDYRTMQLEVLAGNHSLVTTVVENGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVI 459
           I+N++R  + E+LAG  SLVT + ++G  F LD   VYWNSRL  E +RL + F    VI
Sbjct: 180 ITNEFRVPEFEILAGEPSLVTEIKQHGATFRLDYGMVYWNSRLEGEHKRLFAQFKPGQVI 239

Query: 460 CDVFAGVGPISISAARIVKKVYANDLNPYAVEYLERNSVANKLERKIES 505
            D+FAG+GP +I AA+    VYANDLNP +V++L+ NS  NK+   I++
Sbjct: 240 VDMFAGIGPFAIPAAQQGCAVYANDLNPTSVKFLKLNSDINKVGESIKA 277

[Close]

BLAST of CSPI03G41840 vs. ExPASy TrEMBL
Match: A0A0A0LFW2 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G851170 PE=3 SV=1)

HSP 1 Score: 993.8 bits (2568), Expect = 2.6e-286
Identity = 500/503 (99.40%), Postives = 503/503 (100.00%), Query Frame = 0


Query: 1   MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC 60
           MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC
Sbjct: 1   MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC 60

Query: 61  KGIHPFPSVSRQSPIDHSPPLLKTLTGNLERQQLQNQESEGAILDEDAFTRIFDVAALRV 120
           KGIHPFPSVSRQSPIDHSPPLLKTLTGNLER+QLQ+QE+EGAILDEDAFTRIFDVAALRV
Sbjct: 61  KGIHPFPSVSRQSPIDHSPPLLKTLTGNLEREQLQDQENEGAILDEDAFTRIFDVAALRV 120

Query: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL 180
           PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL
Sbjct: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL 180

Query: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM 240
           HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM
Sbjct: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM 240

Query: 241 RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE 300
           RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE
Sbjct: 241 RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE 300

Query: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH 360
           AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH
Sbjct: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH 360

Query: 361 LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420
           LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE
Sbjct: 361 LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420

Query: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND 480
           NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND
Sbjct: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND 480

Query: 481 LNPYAVEYLERNSVANKLERKIE 504
           LNPYAVEYLERNSVANKLERKIE
Sbjct: 481 LNPYAVEYLERNSVANKLERKIE 503

[Close]

BLAST of CSPI03G41840 vs. ExPASy TrEMBL
Match: A0A5A7T001 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold110G001040 PE=3 SV=1)

HSP 1 Score: 955.7 bits (2469), Expect = 7.9e-275
Identity = 481/503 (95.63%), Postives = 493/503 (98.01%), Query Frame = 0


Query: 1   MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC 60
           MVTKLFL PQSLPLTAF+PKFIFP KN LPICFTSPF STTVHSQSQFSHQSL YGPSLC
Sbjct: 1   MVTKLFLTPQSLPLTAFTPKFIFPRKNFLPICFTSPFYSTTVHSQSQFSHQSLPYGPSLC 60

Query: 61  KGIHPFPSVSRQSPIDHSPPLLKTLTGNLERQQLQNQESEGAILDEDAFTRIFDVAALRV 120
           KGIHPFPSVSRQSPIDHS PLLKTLTGNLER+QLQN+ESEGAILDEDAFTRIFDVAALRV
Sbjct: 61  KGIHPFPSVSRQSPIDHS-PLLKTLTGNLEREQLQNEESEGAILDEDAFTRIFDVAALRV 120

Query: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL 180
           PS+GCFELENRLRGHLLNWPRI NIARVPGDEVEAE++ELVGEQNWS+SDDGDGTSIDLL
Sbjct: 121 PSKGCFELENRLRGHLLNWPRIHNIARVPGDEVEAELVELVGEQNWSSSDDGDGTSIDLL 180

Query: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM 240
           HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDE+M
Sbjct: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEEM 240

Query: 241 RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE 300
           RRMGKSEFAIVEVVESEELE+DDMKGLLGD FKGGKWRGSTRLLLLDEQYAAKGV ELPE
Sbjct: 241 RRMGKSEFAIVEVVESEELEVDDMKGLLGDEFKGGKWRGSTRLLLLDEQYAAKGVVELPE 300

Query: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH 360
           AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVL+ALLPKDMIIPSAFETVGHIAH
Sbjct: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLEALLPKDMIIPSAFETVGHIAH 360

Query: 361 LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420
           LNLRDEHL YKKLIAKV+LDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE
Sbjct: 361 LNLRDEHLPYKKLIAKVVLDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420

Query: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND 480
           NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDV+CDVFAGVGPISISAARIVKKVYAND
Sbjct: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVVCDVFAGVGPISISAARIVKKVYAND 480

Query: 481 LNPYAVEYLERNSVANKLERKIE 504
           LNPYAVEYLERNSVANKLERKIE
Sbjct: 481 LNPYAVEYLERNSVANKLERKIE 502

[Close]

BLAST of CSPI03G41840 vs. ExPASy TrEMBL
Match: A0A1S3BZU2 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103494988 PE=3 SV=1)

HSP 1 Score: 955.7 bits (2469), Expect = 7.9e-275
Identity = 481/503 (95.63%), Postives = 493/503 (98.01%), Query Frame = 0


Query: 1   MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC 60
           MVTKLFL PQSLPLTAF+PKFIFP KN LPICFTSPF STTVHSQSQFSHQSL YGPSLC
Sbjct: 1   MVTKLFLTPQSLPLTAFTPKFIFPRKNFLPICFTSPFYSTTVHSQSQFSHQSLPYGPSLC 60

Query: 61  KGIHPFPSVSRQSPIDHSPPLLKTLTGNLERQQLQNQESEGAILDEDAFTRIFDVAALRV 120
           KGIHPFPSVSRQSPIDHS PLLKTLTGNLER+QLQN+ESEGAILDEDAFTRIFDVAALRV
Sbjct: 61  KGIHPFPSVSRQSPIDHS-PLLKTLTGNLEREQLQNEESEGAILDEDAFTRIFDVAALRV 120

Query: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL 180
           PS+GCFELENRLRGHLLNWPRI NIARVPGDEVEAE++ELVGEQNWS+SDDGDGTSIDLL
Sbjct: 121 PSKGCFELENRLRGHLLNWPRIHNIARVPGDEVEAELVELVGEQNWSSSDDGDGTSIDLL 180

Query: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM 240
           HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDE+M
Sbjct: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEEM 240

Query: 241 RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE 300
           RRMGKSEFAIVEVVESEELE+DDMKGLLGD FKGGKWRGSTRLLLLDEQYAAKGV ELPE
Sbjct: 241 RRMGKSEFAIVEVVESEELEVDDMKGLLGDEFKGGKWRGSTRLLLLDEQYAAKGVVELPE 300

Query: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH 360
           AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVL+ALLPKDMIIPSAFETVGHIAH
Sbjct: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLEALLPKDMIIPSAFETVGHIAH 360

Query: 361 LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420
           LNLRDEHL YKKLIAKV+LDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE
Sbjct: 361 LNLRDEHLPYKKLIAKVVLDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420

Query: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND 480
           NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDV+CDVFAGVGPISISAARIVKKVYAND
Sbjct: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVVCDVFAGVGPISISAARIVKKVYAND 480

Query: 481 LNPYAVEYLERNSVANKLERKIE 504
           LNPYAVEYLERNSVANKLERKIE
Sbjct: 481 LNPYAVEYLERNSVANKLERKIE 502

[Close]

BLAST of CSPI03G41840 vs. ExPASy TrEMBL
Match: A0A6J1FD97 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443059 PE=3 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 9.1e-247
Identity = 433/503 (86.08%), Postives = 463/503 (92.05%), Query Frame = 0


Query: 1   MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC 60
           MVTKLFLRPQSLPLTAF+PKFIFP K  LPI FT PFSST VHSQSQFS  + SYGPSL 
Sbjct: 1   MVTKLFLRPQSLPLTAFTPKFIFPSKRFLPIYFTPPFSSTIVHSQSQFSAHTFSYGPSLR 60

Query: 61  KGIHPFPSVSRQSPIDHSPPLLKTLTGNLERQQLQNQESEGAILDEDAFTRIFDVAALRV 120
           KG HPFPSVSRQ P+D+S P++KT+ GN ER++LQN++SE  ILDEDAF RIFD+AALR+
Sbjct: 61  KGYHPFPSVSRQLPLDNSQPVIKTVIGNPERERLQNEKSEEVILDEDAFIRIFDIAALRI 120

Query: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL 180
           PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAE+ ELVGEQN S SDDGDG  ID L
Sbjct: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAELAELVGEQNRSYSDDGDGVDIDSL 180

Query: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM 240
           HRRIYGKAE DGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSR K++KEKKEEG+DE +
Sbjct: 181 HRRIYGKAEDDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRPKKKKEKKEEGKDEGI 240

Query: 241 RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE 300
           +RMGKSEFA+VEVVESEELE+DDMKGLLGD FKGGKWRGSTRLLLLDE+Y  KGVEELPE
Sbjct: 241 KRMGKSEFAVVEVVESEELEVDDMKGLLGDEFKGGKWRGSTRLLLLDERYGDKGVEELPE 300

Query: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH 360
           AIKALLKG+TQE MK TIELVRCRLTL+YDYWQMNEVL+ALLPKDM+IPSAFETVGHIAH
Sbjct: 301 AIKALLKGETQESMKSTIELVRCRLTLYYDYWQMNEVLEALLPKDMVIPSAFETVGHIAH 360

Query: 361 LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420
           LNLRDEHL YKKLIAKV+LDKNKPK+QTVVNK D I+NDYRTMQLEVLAGNHSLVTTVVE
Sbjct: 361 LNLRDEHLPYKKLIAKVVLDKNKPKMQTVVNKIDVINNDYRTMQLEVLAGNHSLVTTVVE 420

Query: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND 480
           NGLRFHLDLATVYWNSRLATER RLLS FTC DV+CDVFAGVGPISISAARIVKKVYAND
Sbjct: 421 NGLRFHLDLATVYWNSRLATERHRLLSGFTCKDVVCDVFAGVGPISISAARIVKKVYAND 480

Query: 481 LNPYAVEYLERNSVANKLERKIE 504
           LNP AVEYLERNSV NKLERKIE
Sbjct: 481 LNPNAVEYLERNSVLNKLERKIE 503

[Close]

BLAST of CSPI03G41840 vs. ExPASy TrEMBL
Match: A0A6J1IJI4 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111476849 PE=3 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 8.0e-243
Identity = 431/503 (85.69%), Postives = 460/503 (91.45%), Query Frame = 0


Query: 1   MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC 60
           MVTKLFLRPQSLPLTAF+PKFIFP K  LPI FT PF ST +HSQSQFS  + SYGPSL 
Sbjct: 1   MVTKLFLRPQSLPLTAFTPKFIFPSKRFLPIYFTPPF-STIIHSQSQFSGHTFSYGPSLR 60

Query: 61  KGIHPFPSVSRQSPIDHSPPLLKTLTGNLERQQLQNQESEGAILDEDAFTRIFDVAALRV 120
           KG H FPSVSRQ P+D+S P++KT+ GN ER++LQN++SE  ILDEDAF RIFD+AALR+
Sbjct: 61  KGHHRFPSVSRQLPLDNSQPVIKTVIGNPERERLQNEKSEEVILDEDAFIRIFDIAALRI 120

Query: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL 180
            SRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAE+ ELVGEQN S SDDGDG  ID L
Sbjct: 121 TSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAELAELVGEQNRSYSDDGDGVDIDSL 180

Query: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM 240
           HRRIYGKAE DGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSR K++KEKKEEG+DE +
Sbjct: 181 HRRIYGKAEDDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRPKKKKEKKEEGKDEGI 240

Query: 241 RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE 300
           +RMGKSEF++VEVVESEELE DDMKGLLGD FKGGKWRGSTRLLLLDE+YA KGVEELPE
Sbjct: 241 KRMGKSEFSVVEVVESEELEGDDMKGLLGDEFKGGKWRGSTRLLLLDERYADKGVEELPE 300

Query: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH 360
           AIKALLKG+TQE MK TIELVRCRLTL+YDYWQMNEVL+ALLPKDMIIPSAFETVGHIAH
Sbjct: 301 AIKALLKGETQESMKSTIELVRCRLTLYYDYWQMNEVLEALLPKDMIIPSAFETVGHIAH 360

Query: 361 LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420
           LNLRDEHL YKKLIAKV+LDKNKPKIQTVVNK D I+NDYRTMQLEVLAGNHSLVTTVVE
Sbjct: 361 LNLRDEHLPYKKLIAKVVLDKNKPKIQTVVNKIDVINNDYRTMQLEVLAGNHSLVTTVVE 420

Query: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND 480
           NGLRFHLDLATVYWNSRLATER RLLS FTC DV+CDVFAGVGPISISAARIVKKVYAND
Sbjct: 421 NGLRFHLDLATVYWNSRLATERHRLLSGFTCKDVVCDVFAGVGPISISAARIVKKVYAND 480

Query: 481 LNPYAVEYLERNSVANKLERKIE 504
           LNP AVEYLERNSV NKLERKIE
Sbjct: 481 LNPNAVEYLERNSVLNKLERKIE 502

[Close]

BLAST of CSPI03G41840 vs. NCBI nr
Match: KAE8651303.1 (hypothetical protein Csa_001327 [Cucumis sativus])

HSP 1 Score: 996.5 bits (2575), Expect = 8.3e-287
Identity = 501/505 (99.21%), Postives = 504/505 (99.80%), Query Frame = 0


Query: 1   MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC 60
           MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC
Sbjct: 1   MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC 60

Query: 61  KGIHPFPSVSRQSPIDHSPPLLKTLTGNLERQQLQNQESEGAILDEDAFTRIFDVAALRV 120
           KGIHPFPSVSRQSPIDHSPPLLKTLTGNLER+QLQ+QE+EGAILDEDAFTRIFDVAALRV
Sbjct: 61  KGIHPFPSVSRQSPIDHSPPLLKTLTGNLEREQLQDQENEGAILDEDAFTRIFDVAALRV 120

Query: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL 180
           PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL
Sbjct: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL 180

Query: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM 240
           HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM
Sbjct: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM 240

Query: 241 RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE 300
           RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE
Sbjct: 241 RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE 300

Query: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH 360
           AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH
Sbjct: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH 360

Query: 361 LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420
           LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE
Sbjct: 361 LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420

Query: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND 480
           NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND
Sbjct: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND 480

Query: 481 LNPYAVEYLERNSVANKLERKIESG 506
           LNPYAVEYLERNSVANKLERKIE G
Sbjct: 481 LNPYAVEYLERNSVANKLERKIEEG 505

[Close]

BLAST of CSPI03G41840 vs. NCBI nr
Match: XP_031738903.1 (tRNA (guanine(37)-N1)-methyltransferase 1 isoform X2 [Cucumis sativus])

HSP 1 Score: 993.8 bits (2568), Expect = 5.4e-286
Identity = 500/503 (99.40%), Postives = 503/503 (100.00%), Query Frame = 0


Query: 1   MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC 60
           MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC
Sbjct: 1   MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC 60

Query: 61  KGIHPFPSVSRQSPIDHSPPLLKTLTGNLERQQLQNQESEGAILDEDAFTRIFDVAALRV 120
           KGIHPFPSVSRQSPIDHSPPLLKTLTGNLER+QLQ+QE+EGAILDEDAFTRIFDVAALRV
Sbjct: 61  KGIHPFPSVSRQSPIDHSPPLLKTLTGNLEREQLQDQENEGAILDEDAFTRIFDVAALRV 120

Query: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL 180
           PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL
Sbjct: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL 180

Query: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM 240
           HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM
Sbjct: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM 240

Query: 241 RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE 300
           RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE
Sbjct: 241 RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE 300

Query: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH 360
           AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH
Sbjct: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH 360

Query: 361 LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420
           LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE
Sbjct: 361 LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420

Query: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND 480
           NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND
Sbjct: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND 480

Query: 481 LNPYAVEYLERNSVANKLERKIE 504
           LNPYAVEYLERNSVANKLERKIE
Sbjct: 481 LNPYAVEYLERNSVANKLERKIE 503

[Close]

BLAST of CSPI03G41840 vs. NCBI nr
Match: XP_004146841.1 (tRNA (guanine(37)-N1)-methyltransferase 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 993.8 bits (2568), Expect = 5.4e-286
Identity = 500/503 (99.40%), Postives = 503/503 (100.00%), Query Frame = 0


Query: 1   MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC 60
           MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC
Sbjct: 1   MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC 60

Query: 61  KGIHPFPSVSRQSPIDHSPPLLKTLTGNLERQQLQNQESEGAILDEDAFTRIFDVAALRV 120
           KGIHPFPSVSRQSPIDHSPPLLKTLTGNLER+QLQ+QE+EGAILDEDAFTRIFDVAALRV
Sbjct: 61  KGIHPFPSVSRQSPIDHSPPLLKTLTGNLEREQLQDQENEGAILDEDAFTRIFDVAALRV 120

Query: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL 180
           PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL
Sbjct: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL 180

Query: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM 240
           HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM
Sbjct: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM 240

Query: 241 RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE 300
           RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE
Sbjct: 241 RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE 300

Query: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH 360
           AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH
Sbjct: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH 360

Query: 361 LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420
           LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE
Sbjct: 361 LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420

Query: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND 480
           NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND
Sbjct: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND 480

Query: 481 LNPYAVEYLERNSVANKLERKIE 504
           LNPYAVEYLERNSVANKLERKIE
Sbjct: 481 LNPYAVEYLERNSVANKLERKIE 503

[Close]

BLAST of CSPI03G41840 vs. NCBI nr
Match: KAA0036752.1 (tRNA (guanine(37)-N1)-methyltransferase 1 [Cucumis melo var. makuwa] >TYJ95819.1 tRNA (guanine(37)-N1)-methyltransferase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 955.7 bits (2469), Expect = 1.6e-274
Identity = 481/503 (95.63%), Postives = 493/503 (98.01%), Query Frame = 0


Query: 1   MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC 60
           MVTKLFL PQSLPLTAF+PKFIFP KN LPICFTSPF STTVHSQSQFSHQSL YGPSLC
Sbjct: 1   MVTKLFLTPQSLPLTAFTPKFIFPRKNFLPICFTSPFYSTTVHSQSQFSHQSLPYGPSLC 60

Query: 61  KGIHPFPSVSRQSPIDHSPPLLKTLTGNLERQQLQNQESEGAILDEDAFTRIFDVAALRV 120
           KGIHPFPSVSRQSPIDHS PLLKTLTGNLER+QLQN+ESEGAILDEDAFTRIFDVAALRV
Sbjct: 61  KGIHPFPSVSRQSPIDHS-PLLKTLTGNLEREQLQNEESEGAILDEDAFTRIFDVAALRV 120

Query: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL 180
           PS+GCFELENRLRGHLLNWPRI NIARVPGDEVEAE++ELVGEQNWS+SDDGDGTSIDLL
Sbjct: 121 PSKGCFELENRLRGHLLNWPRIHNIARVPGDEVEAELVELVGEQNWSSSDDGDGTSIDLL 180

Query: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM 240
           HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDE+M
Sbjct: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEEM 240

Query: 241 RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE 300
           RRMGKSEFAIVEVVESEELE+DDMKGLLGD FKGGKWRGSTRLLLLDEQYAAKGV ELPE
Sbjct: 241 RRMGKSEFAIVEVVESEELEVDDMKGLLGDEFKGGKWRGSTRLLLLDEQYAAKGVVELPE 300

Query: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH 360
           AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVL+ALLPKDMIIPSAFETVGHIAH
Sbjct: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLEALLPKDMIIPSAFETVGHIAH 360

Query: 361 LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420
           LNLRDEHL YKKLIAKV+LDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE
Sbjct: 361 LNLRDEHLPYKKLIAKVVLDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420

Query: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND 480
           NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDV+CDVFAGVGPISISAARIVKKVYAND
Sbjct: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVVCDVFAGVGPISISAARIVKKVYAND 480

Query: 481 LNPYAVEYLERNSVANKLERKIE 504
           LNPYAVEYLERNSVANKLERKIE
Sbjct: 481 LNPYAVEYLERNSVANKLERKIE 502

[Close]

BLAST of CSPI03G41840 vs. NCBI nr
Match: XP_008454621.1 (PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 1 [Cucumis melo])

HSP 1 Score: 955.7 bits (2469), Expect = 1.6e-274
Identity = 481/503 (95.63%), Postives = 493/503 (98.01%), Query Frame = 0


Query: 1   MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC 60
           MVTKLFL PQSLPLTAF+PKFIFP KN LPICFTSPF STTVHSQSQFSHQSL YGPSLC
Sbjct: 1   MVTKLFLTPQSLPLTAFTPKFIFPRKNFLPICFTSPFYSTTVHSQSQFSHQSLPYGPSLC 60

Query: 61  KGIHPFPSVSRQSPIDHSPPLLKTLTGNLERQQLQNQESEGAILDEDAFTRIFDVAALRV 120
           KGIHPFPSVSRQSPIDHS PLLKTLTGNLER+QLQN+ESEGAILDEDAFTRIFDVAALRV
Sbjct: 61  KGIHPFPSVSRQSPIDHS-PLLKTLTGNLEREQLQNEESEGAILDEDAFTRIFDVAALRV 120

Query: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL 180
           PS+GCFELENRLRGHLLNWPRI NIARVPGDEVEAE++ELVGEQNWS+SDDGDGTSIDLL
Sbjct: 121 PSKGCFELENRLRGHLLNWPRIHNIARVPGDEVEAELVELVGEQNWSSSDDGDGTSIDLL 180

Query: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM 240
           HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDE+M
Sbjct: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEEM 240

Query: 241 RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE 300
           RRMGKSEFAIVEVVESEELE+DDMKGLLGD FKGGKWRGSTRLLLLDEQYAAKGV ELPE
Sbjct: 241 RRMGKSEFAIVEVVESEELEVDDMKGLLGDEFKGGKWRGSTRLLLLDEQYAAKGVVELPE 300

Query: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH 360
           AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVL+ALLPKDMIIPSAFETVGHIAH
Sbjct: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLEALLPKDMIIPSAFETVGHIAH 360

Query: 361 LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420
           LNLRDEHL YKKLIAKV+LDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE
Sbjct: 361 LNLRDEHLPYKKLIAKVVLDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420

Query: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND 480
           NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDV+CDVFAGVGPISISAARIVKKVYAND
Sbjct: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVVCDVFAGVGPISISAARIVKKVYAND 480

Query: 481 LNPYAVEYLERNSVANKLERKIE 504
           LNPYAVEYLERNSVANKLERKIE
Sbjct: 481 LNPYAVEYLERNSVANKLERKIE 502

[Close]

BLAST of CSPI03G41840 vs. TAIR 10
Match: AT4G27340.1 (Met-10+ like family protein )

HSP 1 Score: 539.7 bits (1389), Expect = 2.6e-153
Identity = 294/514 (57.20%), Postives = 379/514 (73.74%), Query Frame = 0


Query: 1   MVTKLFL-RPQSLP---LTAFSPKFI---FPHKNSLPIC-FTSPFSSTTVHSQSQFSHQS 60
           MV+KL L R  SLP   ++++S +FI   +P   +L  C F+S  SSTT           
Sbjct: 1   MVSKLSLFRANSLPFPVISSYSARFILKPYPKPKTLIFCVFSSNLSSTT----------- 60

Query: 61  LSYGPSLCKGIHPFPSVSRQSPIDHSPPLLKTLTGNLERQQLQNQESEGAILDEDAFTRI 120
             YGPSL KG  P     R + I       +   G     +      +  +L+ED FTR+
Sbjct: 61  FPYGPSLLKGKKPVLDDIRLASIGRDRDAHRGKIG-----EFDESIEKDVLLNEDEFTRV 120

Query: 121 FDVAALRVPSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDG 180
           F+++A+RVP++ CF LENRLRGHLLNWPRIRNIARVPGDE+E ++++L+G +     +D 
Sbjct: 121 FEISAIRVPAKDCFALENRLRGHLLNWPRIRNIARVPGDEIEEDVVKLLGRETDEEEEDE 180

Query: 181 DGTSIDLLHRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRK--E 240
           D   +D ++RRI GKAEGDGE LS VL+R+KL +TF+S GY+ FR+LAK+SR KR++  E
Sbjct: 181 DSV-VDSVNRRIRGKAEGDGERLSSVLHRDKLARTFNSTGYLKFRNLAKISRPKRKRKTE 240

Query: 241 KKEEGRDEKMRRMGKSEFAIVEVVESEELEMDDMKGLLGDGF-KGGKWRGSTRLLLLDEQ 300
           +  EG+++++    ++E A+VEVVE+   E +D +GLLG+G+   G+WRGSTRLLLLDE+
Sbjct: 241 RTREGKEKEIASR-RNEMAVVEVVETRGGE-EDFEGLLGEGYGSRGRWRGSTRLLLLDEK 300

Query: 301 YAAKGVEELPEAIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIP 360
           Y+ + V++LPEAIK L           + ELV+CRLTLFYDYW M E+L+A+LPK MI+P
Sbjct: 301 YSGEEVQDLPEAIKVLFAEAKMADASLSFELVKCRLTLFYDYWPMIEILEAVLPKGMIVP 360

Query: 361 SAFETVGHIAHLNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLA 420
           SAFE VGHIAHLNLRDEHL YK+LIAKV+LDKN+PKIQTVVNK D I ND+RTMQLEVLA
Sbjct: 361 SAFEMVGHIAHLNLRDEHLPYKRLIAKVVLDKNQPKIQTVVNKIDPIHNDFRTMQLEVLA 420

Query: 421 GNHSLVTTVVENGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISA 480
           GNHSLVT VVENGLRFH+DLA VYWNS+L TERQRLL  F  NDV+CDVFAGVGPI+++A
Sbjct: 421 GNHSLVTLVVENGLRFHVDLARVYWNSKLGTERQRLLLGFDQNDVVCDVFAGVGPIALAA 480

Query: 481 ARIVKKVYANDLNPYAVEYLERNSVANKLERKIE 504
           ARIVK+VYANDLNP+AVE++E+NSV NKLE++IE
Sbjct: 481 ARIVKRVYANDLNPHAVEFMEQNSVVNKLEKRIE 495

[Close]

BLAST of CSPI03G41840 vs. TAIR 10
Match: AT3G56120.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 196.8 bits (499), Expect = 4.1e-50
Identity = 104/219 (47.49%), Postives = 143/219 (65.30%), Query Frame = 0


Query: 281 TRLLLLDEQYAAKGVEELPEAIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQA 340
           TRL++L E      + E+PE     LK  ++      +E+V   +TL Y YW  + +L+ 
Sbjct: 52  TRLVILSESVKNADLSEIPEEKLNQLKKLSE------LEVVPHSVTLGYSYWSADHLLKQ 111

Query: 341 LLPKDMIIPSAFETVGHIAHLNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDY 400
           +LP  + IPS+FET+GHIAHLNL DE L +K +IAKVI DKN P+I+T+VNK   ISN++
Sbjct: 112 ILPDGLDIPSSFETIGHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEF 171

Query: 401 RTMQLEVLAGNHSLVTTVVENGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFA 460
           R  + EVLAG + + T V + G RF LD   VYWNSRL  E  RL S+F   + +CD+FA
Sbjct: 172 RVPKFEVLAGENGMETEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFA 231

Query: 461 GVGPISISAARIVKKVYANDLNPYAVEYLERNSVANKLE 500
           G+GP +I AA+    VYANDLNP +V YL+ N+  NK++
Sbjct: 232 GIGPFAIPAAQKGCFVYANDLNPDSVRYLKINAKFNKVD 264

[Close]

BLAST of CSPI03G41840 vs. TAIR 10
Match: AT4G04670.1 (Met-10+ like family protein / kelch repeat-containing protein )

HSP 1 Score: 53.1 bits (126), Expect = 7.4e-07
Identity = 35/108 (32.41%), Postives = 57/108 (52.78%), Query Frame = 0


Query: 398 NDYRTMQLEVLAGNHSLVTTVVENGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICD 457
           N  R   LE+L G++  V    ENG+ +  D     ++    +E+ R+ ++   N+V+ D
Sbjct: 785 NGTRDSTLEILVGDNGWVNH-RENGILYSFDATKCMFSWGNLSEKLRMGNMACENEVVVD 844

Query: 458 VFAGVG----PISISAARIVKKVYANDLNPYAVEYLERNSVANKLERK 502
           +FAG+G    P  + A    K VYA + NP+A+E L RN  AN +  +
Sbjct: 845 LFAGIGYFVLPFLVRAK--AKLVYACEWNPHAIEALRRNVEANSVSER 889

The following BLAST results are available for this feature:

BLAST of CSPI03G41840 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F6H2F8	3.3e-177	64.57	tRNA (guanine(37)-N1)-methyltransferase 1 OS=Vitis vinifera OX=29760 GN=VIT_19s0...	[more]
Q6NQ64	3.7e-152	57.20	tRNA (guanine(37)-N1)-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=At4...	[more]
Q93YU6	5.8e-49	47.49	tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3...	[more]
F4NUJ6	2.9e-48	46.49	tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strai...	[more]
A9T6G5	1.4e-47	45.41	tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPA...	[more]

BLAST of CSPI03G41840 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFW2	2.6e-286	99.40	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G8511...	[more]
A0A5A7T001	7.9e-275	95.63	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3BZU2	7.9e-275	95.63	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103494988 ...	[more]
A0A6J1FD97	9.1e-247	86.08	tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1IJI4	8.0e-243	85.69	tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111476...	[more]

BLAST of CSPI03G41840 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8651303.1	8.3e-287	99.21	hypothetical protein Csa_001327 [Cucumis sativus]	[more]
XP_031738903.1	5.4e-286	99.40	tRNA (guanine(37)-N1)-methyltransferase 1 isoform X2 [Cucumis sativus]	[more]
XP_004146841.1	5.4e-286	99.40	tRNA (guanine(37)-N1)-methyltransferase 1 isoform X1 [Cucumis sativus]	[more]
KAA0036752.1	1.6e-274	95.63	tRNA (guanine(37)-N1)-methyltransferase 1 [Cucumis melo var. makuwa] >TYJ95819.1...	[more]
XP_008454621.1	1.6e-274	95.63	PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 1 [Cucumis melo]	[more]

BLAST of CSPI03G41840 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27340.1	2.6e-153	57.20	Met-10+ like family protein	[more]
AT3G56120.1	4.1e-50	47.49	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT4G04670.1	7.4e-07	32.41	Met-10+ like family protein / kelch repeat-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 222..242
None	No IPR available	COILS	Coil	Coil	coord: 82..102
None	No IPR available	GENE3D	3.30.300.110		coord: 333..411 e-value: 3.5E-24 score: 86.6
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 414..504 e-value: 1.0E-30 score: 108.6
None	No IPR available	PANTHER	PTHR23245	TRNA METHYLTRANSFERASE	coord: 34..503
None	No IPR available	PANTHER	PTHR23245:SF35	TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE 2	coord: 34..503
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 455..503 e-value: 0.00103345 score: 36.6391
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PFAM	PF02475	Met_10	coord: 351..503 e-value: 5.6E-50 score: 169.9
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PROSITE	PS51684	SAM_MT_TRM5_TYW2	coord: 352..505 score: 70.924759
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 348..503

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G41840.1	CSPI03G41840.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002939	tRNA N1-guanine methylation
cellular_component	GO:0005759	mitochondrial matrix
cellular_component	GO:0005634	nucleus
molecular_function	GO:0052906	tRNA (guanine(37)-N(1))-methyltransferase activity