CSPI03G41500 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G41500
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPhospholipase D
LocationChr3: 35618372 .. 35621192 (-)
RNA-Seq ExpressionCSPI03G41500
SyntenyCSPI03G41500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTATTACACAATATATTCTTCAATAAAACTTTGTCATTTTCTCCCTTTGTAAGAAAATTGTTTGAAGTTATGGCAATGGAGGTGAAGCAAAAGTTCTTCCATGGAACGCTTGAGGTTACTGTCTTTCATGCAACGGCATACGCGCCCTCTTCACCTTTGGATGTATGTTATGGATCATAAAAATTCAAATATATAATTTCAGTAAATAGTAGGCGTTTCTAAACATCCATTACAGAAATAGTTTATTTTTTTCTTTGTTTTCGTGACTCTTTTGTGCAGTGCCTATTTGCTGGTGGCAAGTGTAGCTATGTGACAATTAAGATAGACAACAAGGAGGTTGCCCAGACAAGCCATGAACAGGACCGTGTTTGGAACCAGACATTCCGAGTTCTATGTGCACATCCATTGACTTCTACCGTCACCATTACATTGAAAACATCTCGTTCTGTCTTGGGTAAATTCTATATCCAAGCTCAACAGATTTTGAAAGAAGCAAGTTTTATCAATGGTTTCTTTCCGCTTCTTATGGAAAATGGGAAGCCAAGTCCGGAGCTCAAACTTCGATTCATGTTGTGGTTTAAACCAGCAGTATATGAATTAAGCTGGAAAAAGATGCTGGGAAATGGAGAATACAAGGGGCTGAGAAATGCAACGTTTCCTTTAAGGTCCAACTGTCATGTGACACTATATCAAGATGCTCACCACCTCCCTACTTTTCAACCTCCATTTCATGGTTCAAGTACACCAAGAAGGCTATGGGAAGATGTATACAAGGCCATAGATAATGCAAAGCATTTGGTTTACATTGCAGGCTGGTCCTTCAATCCAAAGATGGTGCTGGTAAATCAAAGCAATCAACCTACCTGTTTTGTTTCTTTCTATTTCTTCCGGTTGGAGATGGCTAATACACTTTCAATGATCAGGTGAGGGACTCTCAGACCGATATACCCTATGCTTTAGGAGTAAAGCTTGGAGAGTTGTTGAAGCAGAAGGCAGACGAAGGAGTAGCTGTGAGAATACTGATTTGGGATGATGAAACATCTCTTCCCATCATCAAGAATGCCGGGATAATGAACACACATGATGAAGATGCTCGGGCATATTTCTTGCACTCAAAAGTCATATGTAGGTTGTGCCCCAAATTGCATCCTATGTCTCCACCAATTTTCTCTCATCATCAGAAAACCATAATCGTAGATGCTCAAACTCACATCAATGCACAAAACAGGGAAATTATGAGTTTCATTGGTGGTTTAGATCTTTGTGATGGTCGCTACGATACAGAACAACATTCATTGTTCCATACTTTGAACACAGAATCCCACTGTCGTGATTTCTATCAGACGAGTATATCGGGTGCCAAACTTCAAAAAGGAGGGCCAAGAGAGCCATGGCATGATGTTCACGCTTGTGTAACAGGTGAAGCTGCTTGGGATATATTAACAAACTTTGAGCAACGATGGACTAAGCAATCGGATGCTTCGTTGTTAGTACCGACAAGCATCTTATTAAAATTGATGCCCCAACTCGAATCAAACACAAACCCACAAAAGAATTGGAACGTGCAAGTTTTTCGATCAATTGACCATTTGTCTGCCAGTCAAATGTTCAGAAACATGACCATTGAACGAACCATCCACGAAGCTTATGTTGAAGCTATCAGGCGAGCTGAGAGATTTATTTATATCGAAAACCAATACTTTATTGGAGGGTGTCACTTTTGGGACAGAGATCAACACTGTGGGTGCACAAATTTGATACCGATCGAGATTGCGCTCAAGGTGGCTAATAAGATCAAGGCAAGGGAGAGGTTCGCAGTTTACATAGTGATTCCAATGTGGCCAGAAGGATCACCAGAGTGTGAATCAGTTGAGGATATGCTACATTGGACAAGGCAGACAATGACAATGATGTATAGACTGATTGGAGAGGCAATCCAAGAAACTGGGGAAAAGGCCCATCCAAGAGATTACTTGAATTTCTTTTGCCTTGCAAACAGAGAGGAAGAGAGAAAGTGGGACTTCATTCCCCCACACAGTCCCCAACATGCAACACAGTACGGGAATGCCCAACAGCATCGGAGGTTCATGATCTATGTCCATTCCAAGGTCATGATAGGTAAGAACACTTCATATGTTCTGAATCTCATTTTTAAGCGGCATTTTTAACAAACAGGTAGAGAGCATTTGAACAGACACAAATATCGAATGTGAAACTCACTACCAATTAAGTTGCAAAGAAATAGAAACTAACCATGAACATGTCGGTATTTTCCAGTGGACGATCTGTACATTCTGATTGGATCCGCAAACGTGAATCAAAGGTCCATGGACGGAGAGCGAGATACAGAGATAGCAATGGGATGCTACCAAATAGAAAACGAGGGAGAAGAATTACCCAATGGAAGAGACATTTCAACATTCCGATTGTCGCTTTGGTACGAACATACAGGAGGATTCGAAGAAGTGTTCTTAAACCCAGAAAGTTTGAAATGCGTTGAAAGGGTTCGATCGATAGGGGATAAATCGTGGAAAATTTACAGCGGAGAAGAAGTTGAAGATATGAAAGGGGTTCACATGGTAACGTACCCAGTGAAGGTGAAAGAAGATGGAAGGATGGAGGATTTGGAAGAAAATGGAGGGCATTTTCCTGATACGAAATGCCCAATTAAAGGAAGAAGATCAATGATGTTGCCACCCATTTTCACAACCTAGTATTAGTTTCAATGGGGATGAGCTAACTCTGAAATTGTGTTGAACCTCCATGAATGAGAATTTCCAACTGTTGAATTGTGGATTGGAGGAATCCTTTTCCATTTTCC

mRNA sequence

GTATTACACAATATATTCTTCAATAAAACTTTGTCATTTTCTCCCTTTGTAAGAAAATTGTTTGAAGTTATGGCAATGGAGGTGAAGCAAAAGTTCTTCCATGGAACGCTTGAGGTTACTGTCTTTCATGCAACGGCATACGCGCCCTCTTCACCTTTGGATTGCCTATTTGCTGGTGGCAAGTGTAGCTATGTGACAATTAAGATAGACAACAAGGAGGTTGCCCAGACAAGCCATGAACAGGACCGTGTTTGGAACCAGACATTCCGAGTTCTATGTGCACATCCATTGACTTCTACCGTCACCATTACATTGAAAACATCTCGTTCTGTCTTGGGTAAATTCTATATCCAAGCTCAACAGATTTTGAAAGAAGCAAGTTTTATCAATGGTTTCTTTCCGCTTCTTATGGAAAATGGGAAGCCAAGTCCGGAGCTCAAACTTCGATTCATGTTGTGGTTTAAACCAGCAGTATATGAATTAAGCTGGAAAAAGATGCTGGGAAATGGAGAATACAAGGGGCTGAGAAATGCAACGTTTCCTTTAAGGTCCAACTGTCATGTGACACTATATCAAGATGCTCACCACCTCCCTACTTTTCAACCTCCATTTCATGGTTCAAGTACACCAAGAAGGCTATGGGAAGATGTATACAAGGCCATAGATAATGCAAAGCATTTGGTTTACATTGCAGGCTGGTCCTTCAATCCAAAGATGGTGCTGGTGAGGGACTCTCAGACCGATATACCCTATGCTTTAGGAGTAAAGCTTGGAGAGTTGTTGAAGCAGAAGGCAGACGAAGGAGTAGCTGTGAGAATACTGATTTGGGATGATGAAACATCTCTTCCCATCATCAAGAATGCCGGGATAATGAACACACATGATGAAGATGCTCGGGCATATTTCTTGCACTCAAAAGTCATATGTAGGTTGTGCCCCAAATTGCATCCTATGTCTCCACCAATTTTCTCTCATCATCAGAAAACCATAATCGTAGATGCTCAAACTCACATCAATGCACAAAACAGGGAAATTATGAGTTTCATTGGTGGTTTAGATCTTTGTGATGGTCGCTACGATACAGAACAACATTCATTGTTCCATACTTTGAACACAGAATCCCACTGTCGTGATTTCTATCAGACGAGTATATCGGGTGCCAAACTTCAAAAAGGAGGGCCAAGAGAGCCATGGCATGATGTTCACGCTTGTGTAACAGGTGAAGCTGCTTGGGATATATTAACAAACTTTGAGCAACGATGGACTAAGCAATCGGATGCTTCGTTGTTAGTACCGACAAGCATCTTATTAAAATTGATGCCCCAACTCGAATCAAACACAAACCCACAAAAGAATTGGAACGTGCAAGTTTTTCGATCAATTGACCATTTGTCTGCCAGTCAAATGTTCAGAAACATGACCATTGAACGAACCATCCACGAAGCTTATGTTGAAGCTATCAGGCGAGCTGAGAGATTTATTTATATCGAAAACCAATACTTTATTGGAGGGTGTCACTTTTGGGACAGAGATCAACACTGTGGGTGCACAAATTTGATACCGATCGAGATTGCGCTCAAGGTGGCTAATAAGATCAAGGCAAGGGAGAGGTTCGCAGTTTACATAGTGATTCCAATGTGGCCAGAAGGATCACCAGAGTGTGAATCAGTTGAGGATATGCTACATTGGACAAGGCAGACAATGACAATGATGTATAGACTGATTGGAGAGGCAATCCAAGAAACTGGGGAAAAGGCCCATCCAAGAGATTACTTGAATTTCTTTTGCCTTGCAAACAGAGAGGAAGAGAGAAAGTGGGACTTCATTCCCCCACACAGTCCCCAACATGCAACACAGTACGGGAATGCCCAACAGCATCGGAGGTTCATGATCTATGTCCATTCCAAGGTCATGATAGTGGACGATCTGTACATTCTGATTGGATCCGCAAACGTGAATCAAAGGTCCATGGACGGAGAGCGAGATACAGAGATAGCAATGGGATGCTACCAAATAGAAAACGAGGGAGAAGAATTACCCAATGGAAGAGACATTTCAACATTCCGATTGTCGCTTTGGTACGAACATACAGGAGGATTCGAAGAAGTGTTCTTAAACCCAGAAAGTTTGAAATGCGTTGAAAGGGTTCGATCGATAGGGGATAAATCGTGGAAAATTTACAGCGGAGAAGAAGTTGAAGATATGAAAGGGGTTCACATGGTAACGTACCCAGTGAAGGTGAAAGAAGATGGAAGGATGGAGGATTTGGAAGAAAATGGAGGGCATTTTCCTGATACGAAATGCCCAATTAAAGGAAGAAGATCAATGATGTTGCCACCCATTTTCACAACCTAGTATTAGTTTCAATGGGGATGAGCTAACTCTGAAATTGTGTTGAACCTCCATGAATGAGAATTTCCAACTGTTGAATTGTGGATTGGAGGAATCCTTTTCCATTTTCC

Coding sequence (CDS)

ATGGCAATGGAGGTGAAGCAAAAGTTCTTCCATGGAACGCTTGAGGTTACTGTCTTTCATGCAACGGCATACGCGCCCTCTTCACCTTTGGATTGCCTATTTGCTGGTGGCAAGTGTAGCTATGTGACAATTAAGATAGACAACAAGGAGGTTGCCCAGACAAGCCATGAACAGGACCGTGTTTGGAACCAGACATTCCGAGTTCTATGTGCACATCCATTGACTTCTACCGTCACCATTACATTGAAAACATCTCGTTCTGTCTTGGGTAAATTCTATATCCAAGCTCAACAGATTTTGAAAGAAGCAAGTTTTATCAATGGTTTCTTTCCGCTTCTTATGGAAAATGGGAAGCCAAGTCCGGAGCTCAAACTTCGATTCATGTTGTGGTTTAAACCAGCAGTATATGAATTAAGCTGGAAAAAGATGCTGGGAAATGGAGAATACAAGGGGCTGAGAAATGCAACGTTTCCTTTAAGGTCCAACTGTCATGTGACACTATATCAAGATGCTCACCACCTCCCTACTTTTCAACCTCCATTTCATGGTTCAAGTACACCAAGAAGGCTATGGGAAGATGTATACAAGGCCATAGATAATGCAAAGCATTTGGTTTACATTGCAGGCTGGTCCTTCAATCCAAAGATGGTGCTGGTGAGGGACTCTCAGACCGATATACCCTATGCTTTAGGAGTAAAGCTTGGAGAGTTGTTGAAGCAGAAGGCAGACGAAGGAGTAGCTGTGAGAATACTGATTTGGGATGATGAAACATCTCTTCCCATCATCAAGAATGCCGGGATAATGAACACACATGATGAAGATGCTCGGGCATATTTCTTGCACTCAAAAGTCATATGTAGGTTGTGCCCCAAATTGCATCCTATGTCTCCACCAATTTTCTCTCATCATCAGAAAACCATAATCGTAGATGCTCAAACTCACATCAATGCACAAAACAGGGAAATTATGAGTTTCATTGGTGGTTTAGATCTTTGTGATGGTCGCTACGATACAGAACAACATTCATTGTTCCATACTTTGAACACAGAATCCCACTGTCGTGATTTCTATCAGACGAGTATATCGGGTGCCAAACTTCAAAAAGGAGGGCCAAGAGAGCCATGGCATGATGTTCACGCTTGTGTAACAGGTGAAGCTGCTTGGGATATATTAACAAACTTTGAGCAACGATGGACTAAGCAATCGGATGCTTCGTTGTTAGTACCGACAAGCATCTTATTAAAATTGATGCCCCAACTCGAATCAAACACAAACCCACAAAAGAATTGGAACGTGCAAGTTTTTCGATCAATTGACCATTTGTCTGCCAGTCAAATGTTCAGAAACATGACCATTGAACGAACCATCCACGAAGCTTATGTTGAAGCTATCAGGCGAGCTGAGAGATTTATTTATATCGAAAACCAATACTTTATTGGAGGGTGTCACTTTTGGGACAGAGATCAACACTGTGGGTGCACAAATTTGATACCGATCGAGATTGCGCTCAAGGTGGCTAATAAGATCAAGGCAAGGGAGAGGTTCGCAGTTTACATAGTGATTCCAATGTGGCCAGAAGGATCACCAGAGTGTGAATCAGTTGAGGATATGCTACATTGGACAAGGCAGACAATGACAATGATGTATAGACTGATTGGAGAGGCAATCCAAGAAACTGGGGAAAAGGCCCATCCAAGAGATTACTTGAATTTCTTTTGCCTTGCAAACAGAGAGGAAGAGAGAAAGTGGGACTTCATTCCCCCACACAGTCCCCAACATGCAACACAGTACGGGAATGCCCAACAGCATCGGAGGTTCATGATCTATGTCCATTCCAAGGTCATGATAGTGGACGATCTGTACATTCTGATTGGATCCGCAAACGTGAATCAAAGGTCCATGGACGGAGAGCGAGATACAGAGATAGCAATGGGATGCTACCAAATAGAAAACGAGGGAGAAGAATTACCCAATGGAAGAGACATTTCAACATTCCGATTGTCGCTTTGGTACGAACATACAGGAGGATTCGAAGAAGTGTTCTTAAACCCAGAAAGTTTGAAATGCGTTGAAAGGGTTCGATCGATAGGGGATAAATCGTGGAAAATTTACAGCGGAGAAGAAGTTGAAGATATGAAAGGGGTTCACATGGTAACGTACCCAGTGAAGGTGAAAGAAGATGGAAGGATGGAGGATTTGGAAGAAAATGGAGGGCATTTTCCTGATACGAAATGCCCAATTAAAGGAAGAAGATCAATGATGTTGCCACCCATTTTCACAACCTAG

Protein sequence

MAMEVKQKFFHGTLEVTVFHATAYAPSSPLDCLFAGGKCSYVTIKIDNKEVAQTSHEQDRVWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPSPELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPPFHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIFSHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTSISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQLESNTNPQKNWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIGGCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPECESVEDMLHWTRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYGNAQQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGRDISTFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTYPVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT*
Homology
[Close]
BLAST of CSPI03G41500 vs. ExPASy Swiss-Prot
Match: Q9C888 (Phospholipase D alpha 4 OS=Arabidopsis thaliana OX=3702 GN=PLDALPHA4 PE=2 SV=1)

HSP 1 Score: 971.1 bits (2509), Expect = 7.4e-282
Identity = 460/776 (59.28%), Postives = 593/776 (76.42%), Query Frame = 0 

Query: 1   MAMEVKQKFFHGTLEVTVFHATAYAPSSPLDCLFAGGKCSYVTIKIDNKEVAQTSHEQDR 60
           M +E ++K+FHGTLE+T+F AT ++P  P +C+    K +YVTIKI+ K+VA+TS E DR
Sbjct: 1   MELEEQKKYFHGTLEITIFDATPFSPPFPFNCICTKPKAAYVTIKINKKKVAKTSSEYDR 60

Query: 61  VWNQTFRVLCAHPLT-STVTITLKTSRSVLGKFYIQAQQIL-KEASFINGFFPLLMENGK 120
           +WNQTF++LCAHP+T +T+TITLKT  SVLG+F I A+QIL   ++ INGFFPL+ +NG 
Sbjct: 61  IWNQTFQILCAHPVTDTTITITLKTRCSVLGRFRISAEQILTSNSAVINGFFPLIADNGS 120

Query: 121 PSPELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQ 180
               LKL+ ++WF+PA  E  W + L    ++G+RNA+FP RSNC V LYQDAHH  TF 
Sbjct: 121 TKRNLKLKCLMWFRPAYLEPGWCRALEEASFQGIRNASFPQRSNCRVVLYQDAHHKATFD 180

Query: 181 P-----PFHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVK 240
           P     PF+     R LWEDVYKAI++A+HLVYIAGW+ NP +VLVRD++T+IP+A+GV 
Sbjct: 181 PRVDDVPFNA----RNLWEDVYKAIESARHLVYIAGWALNPNLVLVRDNETEIPHAVGVT 240

Query: 241 LGELLKQKADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLH 300
           +GELLK+K++EGVAVR+++W+DETSLP+IKN G+M T+ E A AYF ++ V+CRLCP+LH
Sbjct: 241 VGELLKRKSEEGVAVRVMLWNDETSLPMIKNKGVMRTNVERALAYFRNTNVVCRLCPRLH 300

Query: 301 PMSPPIFSHHQKTIIVDAQ-THINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESH 360
              P  F+HHQKTI +D + T+ + + REIMSF+GG DLCDGRYDTE+HSLF TL TE+ 
Sbjct: 301 KKLPTAFAHHQKTITLDTRVTNSSTKEREIMSFLGGFDLCDGRYDTEEHSLFRTLGTEA- 360

Query: 361 CRDFYQTSISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSI 420
             DFYQTS++GAKL +GGPREPWHD H  V G AAWD+L NFEQRWTKQ + S+LV TS 
Sbjct: 361 --DFYQTSVAGAKLSRGGPREPWHDCHVSVVGGAAWDVLKNFEQRWTKQCNPSVLVNTSG 420

Query: 421 LLKLMPQLESNTNPQKNWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIY 480
           +  L+          + WNVQV RSIDH+SA++M R + +E+++H+ YV AIR+AERFIY
Sbjct: 421 IRNLVNLTGPTEENNRKWNVQVLRSIDHISATEMPRGLPVEKSVHDGYVAAIRKAERFIY 480

Query: 481 IENQYFIGGCHFWD--RDQHC-GCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSP 540
           IENQYF+G C  W+   D+ C GCTNLIP+EIALK+A KI+ARERFAVYIVIPMWPEG P
Sbjct: 481 IENQYFMGSCDHWESKNDKICSGCTNLIPVEIALKIAAKIRARERFAVYIVIPMWPEGPP 540

Query: 541 ECESVEDMLHWTRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPH 600
           E E+VE++LHWTR+TM+MMY++IGEAI E G+K+HPRDYLNFFCLANREE+R  +F    
Sbjct: 541 ESETVEEILHWTRETMSMMYQIIGEAIWEVGDKSHPRDYLNFFCLANREEKRDGEFEAVS 600

Query: 601 SPQHATQYGNAQQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQI 660
           SP   T Y NAQ++RRFM+YVHSK+MIVDD YILIGSAN+NQRSMDG RDTEIA+GCYQ 
Sbjct: 601 SPHQKTHYWNAQRNRRFMVYVHSKLMIVDDTYILIGSANINQRSMDGCRDTEIAIGCYQT 660

Query: 661 ENEGEELPNGRDISTFRLSLWYEHTGG----FEEVFLNPESLKCVERVRSIGDKSWKIYS 720
                   N  +I  +RLSLWYEHTGG     +     PESL+CV  +R+IG++ W+IYS
Sbjct: 661 NTN-----NTNEIQAYRLSLWYEHTGGKITADDLSSSEPESLECVRGLRTIGEQMWEIYS 720

Query: 721 GEEVEDMKGVHMVTYPVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 762
           G++V DM G+H+V YP+ V  DG +E++ +  G FPDTK  +KG+RS M PP+ TT
Sbjct: 721 GDKVVDMLGIHLVAYPISVTGDGAVEEVGD--GCFPDTKTLVKGKRSKMFPPVLTT 762

[Close]
BLAST of CSPI03G41500 vs. ExPASy Swiss-Prot
Match: Q43270 (Phospholipase D alpha 1 OS=Zea mays OX=4577 GN=PLD1 PE=2 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 1.5e-178
Identity = 339/815 (41.60%), Postives = 480/815 (58.90%), Query Frame = 0 

Query: 7   QKFFHGTLEVTVF---------HATAYAPS------SPLDCLFAGGKCS---YVTIKIDN 66
           Q   HGTL  T+F          AT  AP         ++     GK +   Y T+ ++ 
Sbjct: 3   QILLHGTLHATIFEAESLSNPHRATGGAPKFIRKLVEGIEDTVGVGKGATKIYATVDLEK 62

Query: 67  KEVAQT----SHEQDRVWNQTFRVLCAHPLTSTVTITLKTSRSV----LGKFYIQAQQIL 126
             V +T    +   +  W ++F + CAH + + V  T+K   S+    +G+ Y+  Q +L
Sbjct: 63  ARVGRTRMISNEPVNPRWYESFHIYCAH-MAADVIFTVKIDNSIGASLIGRAYLAVQDLL 122

Query: 127 KEASFINGFFPLLMENGKPSPELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLR 186
                I+ +  +  EN +P  + K+   L +     + +W + + + +Y G+    F  R
Sbjct: 123 -GGEEIDKWLEISDENREPVGDSKIHVKLQYFDVGKDRNWARGVRSTKYPGVPYTFFSQR 182

Query: 187 SNCHVTLYQDAH----HLPTFQPPFHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKM 246
             C VTLYQDAH     +P  Q     +  P R WED++ AI  A+HL+YI GWS   ++
Sbjct: 183 QGCKVTLYQDAHVPDNFVPRIQLADGKNYEPHRCWEDIFDAISKAQHLIYITGWSVYTEI 242

Query: 247 VLVRDSQTDIPYALGVKLGELLKQKADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDAR 306
            LVRD+    P    V LGELLK+KA EGV V +L+WDD TS+ ++K  G+M THDE+  
Sbjct: 243 TLVRDTNRPKPGG-DVTLGELLKRKASEGVRVLMLVWDDRTSVGLLKKDGLMATHDEETA 302

Query: 307 AYFLHSKVICRLCPKLHPMS---------PPIFSHHQKTIIVDAQ-THINAQNREIMSFI 366
            YF  + V C LCP+    S           +F+HHQK ++VD +  +  +Q R I+SFI
Sbjct: 303 NYFHGTDVNCVLCPRNPDDSGSFVQDLQISTMFTHHQKIVVVDHEMPNQGSQQRRIVSFI 362

Query: 367 GGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTSISGAKLQKGGPREPWHDVHACVTGEA 426
           GG+DLCDGRYDT+ HSLF TL+T  H  DF+Q +  G  ++KGGPREPWHD+H+ + G  
Sbjct: 363 GGIDLCDGRYDTQYHSLFRTLDTVHH-DDFHQPNFEGGSIKKGGPREPWHDIHSRLEGPI 422

Query: 427 AWDILTNFEQRWTKQSDASLLVPTSIL--LKLMPQLESNTNPQKNWNVQVFRSIDHLSAS 486
           AWD+L NFEQRW KQ    LLV    L  + + P        ++ WNVQ+FRSID  +A 
Sbjct: 423 AWDVLYNFEQRWRKQGGKDLLVRLRDLPDIIIPPSPVMFPEDRETWNVQLFRSIDGGAAF 482

Query: 487 QM--------------FRNMTIERTIHEAYVEAIRRAERFIYIENQYFIGGCHFWD---- 546
                            ++  I+R+I +AYV AIRRA+ FIYIENQYF+G  + W     
Sbjct: 483 GFPETPEEAARAGLVSGKDQIIDRSIQDAYVNAIRRAKNFIYIENQYFLGSSYGWKPEGI 542

Query: 547 RDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPECESVEDMLHWTRQTMT 606
           + +  G  +LIP E++LK+ +KI+A ERF VY+V+PMWPEG PE  SV+ +L W R+TM 
Sbjct: 543 KPEEIGALHLIPKELSLKIVSKIEAGERFTVYVVVPMWPEGVPESASVQAILDWQRRTME 602

Query: 607 MMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYGNAQQHRRF 666
           MMY  I +A++  G +A+P+DYL FFCL NRE +++ ++ P   P+  T Y  AQ+ RRF
Sbjct: 603 MMYTDIAQALEANGIEANPKDYLTFFCLGNREVKQEGEYEPEEHPEPDTDYIRAQEARRF 662

Query: 667 MIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGRDISTFR 726
           MIYVH+K+MIVDD YI+IGSAN+NQRSMDG RD+EIAMG YQ  +     P    I  FR
Sbjct: 663 MIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGAYQPYHLATRQPARGQIHGFR 722

Query: 727 LSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTYPVKVKE 762
           +SLWYEH G  E+VF  PES++CV++V  + +K W +YS +++E     H+++YP+ V  
Sbjct: 723 MSLWYEHLGMLEDVFQRPESVECVQKVNEVAEKYWDLYSSDDLEQDLPGHLLSYPIGVTA 782

[Close]
BLAST of CSPI03G41500 vs. ExPASy Swiss-Prot
Match: Q43007 (Phospholipase D alpha 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PLD1 PE=1 SV=2)

HSP 1 Score: 627.5 bits (1617), Expect = 2.0e-178
Identity = 341/815 (41.84%), Postives = 481/815 (59.02%), Query Frame = 0 

Query: 7   QKFFHGTLEVTVFHATAY------APSSP---------LDCLFAGGKCS---YVTIKIDN 66
           Q   HGTL  T+F A +       + S+P         ++     GK +   Y TI ++ 
Sbjct: 3   QMLLHGTLHATIFEAASLSNPHRASGSAPKFIRKFVEGIEDTVGVGKGATKVYSTIDLEK 62

Query: 67  KEVAQ----TSHEQDRVWNQTFRVLCAHPLTSTVTITLKTSRSV----LGKFYIQAQQIL 126
             V +    T+   +  W ++F + CAH + S V  T+K    +    +G+ Y+  Q++L
Sbjct: 63  ARVGRTRMITNEPINPRWYESFHIYCAH-MASNVIFTVKIDNPIGATNIGRAYLPVQELL 122

Query: 127 KEASFINGFFPLLMENGKPSPELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLR 186
                I+ +  +   N +P  E K+   L +     + +W + + + +Y G+    F  R
Sbjct: 123 -NGEEIDRWLDICDNNREPVGESKIHVKLQYFDVSKDRNWARGVRSTKYPGVPYTFFSQR 182

Query: 187 SNCHVTLYQDAHHLPTFQPPF----HGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKM 246
             C VTLYQDAH    F P        +  P R WED++ AI NA+HL+YI GWS   ++
Sbjct: 183 QGCKVTLYQDAHVPDNFIPKIPLADGKNYEPHRCWEDIFDAISNAQHLIYITGWSVYTEI 242

Query: 247 VLVRDSQTDIPYALGVKLGELLKQKADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDAR 306
            LVRDS    P    V LGELLK+KA EGV V +L+WDD TS+ ++K  G+M THDE+  
Sbjct: 243 TLVRDSNRPKPGG-DVTLGELLKKKASEGVRVLMLVWDDRTSVGLLKRDGLMATHDEETE 302

Query: 307 AYFLHSKVICRLCPKLHPMSPPI---------FSHHQKTIIVDAQ-THINAQNREIMSFI 366
            YF  S V C LCP+    S  I         F+HHQK ++VD +  +  +Q R I+SF+
Sbjct: 303 NYFHGSDVNCVLCPRNPDDSGSIVQDLSISTMFTHHQKIVVVDHELPNQGSQQRRIVSFV 362

Query: 367 GGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTSISGAKLQKGGPREPWHDVHACVTGEA 426
           GGLDLCDGRYDT+ HSLF TL++ +H  DF+Q + + A ++KGGPREPWHD+H+ + G  
Sbjct: 363 GGLDLCDGRYDTQYHSLFRTLDS-THHDDFHQPNFATASIKKGGPREPWHDIHSRLEGPI 422

Query: 427 AWDILTNFEQRWTKQSDASLLVPTSILLK--LMPQLESNTNPQKNWNVQVFRSIDHLSAS 486
           AWD+L NFEQRW KQ    LL+    L    + P        ++ WNVQ+FRSID  +A 
Sbjct: 423 AWDVLYNFEQRWRKQGGKDLLLQLRDLSDTIIPPSPVMFPEDRETWNVQLFRSIDGGAAF 482

Query: 487 QM--------------FRNMTIERTIHEAYVEAIRRAERFIYIENQYFIGGCHFWD---- 546
                            ++  I+R+I +AY+ AIRRA+ FIYIENQYF+G  + W     
Sbjct: 483 GFPDTPEEAAKAGLVSGKDQIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSYAWKPEGI 542

Query: 547 RDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPECESVEDMLHWTRQTMT 606
           + +  G  +LIP E+ALKV +KI+A ERF VY+V+PMWPEG PE  SV+ +L W R+TM 
Sbjct: 543 KPEDIGALHLIPKELALKVVSKIEAGERFTVYVVVPMWPEGVPESGSVQAILDWQRRTME 602

Query: 607 MMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYGNAQQHRRF 666
           MMY  I EA+Q  G +A+P+DYL FFCL NRE ++  ++ P   P+  T Y  AQ+ RRF
Sbjct: 603 MMYTDITEALQAKGIEANPKDYLTFFCLGNREVKQAGEYQPEEQPEADTDYSRAQEARRF 662

Query: 667 MIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGRDISTFR 726
           MIYVH+K+MIVDD YI+IGSAN+NQRSMDG RD+EIAMG YQ  +     P    I  FR
Sbjct: 663 MIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPYHLATRQPARGQIHGFR 722

Query: 727 LSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTYPVKVKE 762
           ++LWYEH G  ++VF  PESL+CV++V  I +K W +YS ++++     H+++YP+ V  
Sbjct: 723 MALWYEHLGMLDDVFQRPESLECVQKVNRIAEKYWDMYSSDDLQQDLPGHLLSYPIGVAS 782

[Close]
BLAST of CSPI03G41500 vs. ExPASy Swiss-Prot
Match: P93400 (Phospholipase D alpha 1 OS=Nicotiana tabacum OX=4097 GN=PLD1 PE=2 SV=2)

HSP 1 Score: 616.7 bits (1589), Expect = 3.5e-175
Identity = 326/770 (42.34%), Postives = 463/770 (60.13%), Query Frame = 0 

Query: 36  GGKCSYVTIKIDNKEVAQT----SHEQDRVWNQTFRVLCAHPLTSTVTITLKTSR----S 95
           G    Y T+ ++   V +T    +   +  W ++F + CAH + S V  T+K       +
Sbjct: 45  GTPAIYATVDLEKARVGRTRKIKNEPNNPRWYESFHIYCAH-MASNVIFTVKDDNPIGAT 104

Query: 96  VLGKFYIQAQQILKEASFINGFFPLLMENGKPSPE-LKLRFMLWFKPAVYELSWKKMLGN 155
           ++G+ Y+  +++L E   I+ +  +L     P  E  K+   L F     + +W++ + +
Sbjct: 105 LIGRAYVPVEELL-EGEEIDKWVEILDREMNPIAEGSKIHVKLQFFDVSRDPNWERGIRS 164

Query: 156 GEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQP--PFHGSS--TPRRLWEDVYKAIDNAK 215
            +Y G+    F  R+ C V+LYQDAH    F P  P  G     P R WED++ AI NAK
Sbjct: 165 SKYPGVPYTFFAQRTGCRVSLYQDAHVPDNFIPKIPLSGGKYYEPHRCWEDIFDAIINAK 224

Query: 216 HLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKADEGVAVRILIWDDETSLPII 275
           HL+YI GWS   ++ LVRDS+   P    + LGELLK+KA EGV V +L+WDD TS+ ++
Sbjct: 225 HLIYITGWSVYTEITLVRDSRRQKPGG-DITLGELLKKKASEGVKVLMLVWDDRTSVGLL 284

Query: 276 KNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPI---------FSHHQKTIIVDAQT 335
           K  G+M THD++   +F  ++V C LCP+       I         F+HHQK ++VD++ 
Sbjct: 285 KKDGLMATHDQETEQFFQGTEVNCVLCPRNPDDGGSIVQSLQIGTMFTHHQKIVVVDSEL 344

Query: 336 HI-NAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTSISGAKLQKGGPR 395
               ++ R I+SF+GG+DLCDGRYDT  HSLF TL+T +H  DF+Q +     + KGGPR
Sbjct: 345 PSGESEKRRILSFVGGIDLCDGRYDTPFHSLFRTLDT-AHHDDFHQPNFPDGAITKGGPR 404

Query: 396 EPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSIL--LKLMPQLESNTNPQKNW 455
           EPWHD+H+ + G  AWD+L NFEQRW KQ    +LV    L  + + P    + +  + W
Sbjct: 405 EPWHDIHSRLEGPIAWDVLFNFEQRWRKQGGKDVLVNFRELDDIIIPPSPVMHLDDSETW 464

Query: 456 NVQVFRSIDHLSASQM--------------FRNMTIERTIHEAYVEAIRRAERFIYIENQ 515
           NVQ+FRSID  +A                   +  I+R+I +AY+ AIRRA+ FIYIENQ
Sbjct: 465 NVQLFRSIDEGAAFGFPETPEDAAKAGLVSGXDNIIDRSIQDAYIHAIRRAKNFIYIENQ 524

Query: 516 YFIGGCHFWDRD----QHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPECE 575
           YF+G  + W  D    +  G  ++IP E+ALK+ +KI+A ERF VY+V+PMWPEG PE  
Sbjct: 525 YFLGSSYDWQSDDIKVEDIGALHVIPKELALKIVSKIEAGERFTVYVVVPMWPEGIPESA 584

Query: 576 SVEDMLHWTRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQ 635
           SV+ +L W R+TM MMY+ I +A+   G +  PR+YL FFC+ NRE ++   + P  +P+
Sbjct: 585 SVQAILDWQRRTMEMMYKHIVQALNAKGIEEDPRNYLTFFCIGNREVKKSGAYEPSETPE 644

Query: 636 HATQYGNAQQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENE 695
             + Y  AQ+ RRFMIYVHSK+MIVDD YI++GSAN+NQRSMDG RD+EIAMG YQ  + 
Sbjct: 645 PDSDYIRAQEARRFMIYVHSKMMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPHHL 704

Query: 696 GEELPNGRDISTFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVE-D 755
               P    I  FR++LWYEH G  +E FL+PES +CV +V  + DK W +YS E +E D
Sbjct: 705 ATREPARGQIHGFRMALWYEHLGMLDETFLHPESEECVSKVNRMADKYWDLYSSESLERD 764

Query: 756 MKGVHMVTYPVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 762
           + G H++ YP+ V  +G + +L     HFPDTK  + G +S  LPPI TT
Sbjct: 765 LPG-HLLRYPIGVASEGDVTEL-PGAEHFPDTKARVLGTKSDYLPPILTT 808

[Close]
BLAST of CSPI03G41500 vs. ExPASy Swiss-Prot
Match: Q41142 (Phospholipase D alpha 1 OS=Ricinus communis OX=3988 GN=PLD1 PE=1 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 8.7e-174
Identity = 328/765 (42.88%), Postives = 462/765 (60.39%), Query Frame = 0 

Query: 41  YVTIKIDNKEVAQT----SHEQDRVWNQTFRVLCAHPLTSTVTITLKTSR----SVLGKF 100
           Y TI ++   V +T    + + +  W ++F V CAH   S V  T+K       +++G+ 
Sbjct: 50  YATIDLEKARVGRTRILENEQSNPRWYESFHVYCAHQ-ASNVIFTVKDDNPIGATLIGRA 109

Query: 101 YIQAQQILKEASFINGFFPLLMENGKP-SPELKLRFMLWFKPAVYELSWKKMLGNGEYKG 160
           Y+  +++L +   I+ +  +L E+  P     K+   L +     + +W + + + +Y G
Sbjct: 110 YVPVEELL-DGEEIDRWVEILDEDKNPVHSGSKIHVKLQYFEVTKDRNWGQGIRSSKYPG 169

Query: 161 LRNATFPLRSNCHVTLYQDAHHLPTFQP--PFHGSS--TPRRLWEDVYKAIDNAKHLVYI 220
           +    F  R  C V+LYQDAH    F P  P  G +   P R WEDV+ AI NAKHL+YI
Sbjct: 170 VPYTYFSQRQGCKVSLYQDAHIPDKFVPQIPLAGGNYYEPHRCWEDVFDAITNAKHLIYI 229

Query: 221 AGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKADEGVAVRILIWDDETSLPIIKNAGI 280
            GWS   ++ L+RDS+   P    + LGELLK+KA EGV V +L+WDD TS+ ++K  G+
Sbjct: 230 TGWSVYTEISLIRDSRRPKPGG-DITLGELLKKKASEGVRVLMLVWDDRTSVGLLKKDGL 289

Query: 281 MNTHDEDARAYFLHSKVICRLCPKLHP----------MSPPIFSHHQKTIIVD-AQTHIN 340
           M THDE+   +F ++ V C LCP+ +P              +F+HHQK ++VD A  + +
Sbjct: 290 MATHDEETEHFFQNTDVHCVLCPR-NPDDGGSFVQDLQISTMFTHHQKIVVVDSAMPNGD 349

Query: 341 AQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTSISGAKLQKGGPREPWH 400
           +Q R I+SF+GGLDLCDGRYD+  HSLF TL++ +H  DF+Q + +GA ++KGGPREPWH
Sbjct: 350 SQRRRIVSFVGGLDLCDGRYDSPFHSLFRTLDS-AHHDDFHQPNFAGASIEKGGPREPWH 409

Query: 401 DVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSIL--LKLMPQLESNTNPQKNWNVQV 460
           D+H+ + G  AWD+L NFEQRW KQ    LL+    L  + + P      +  + WNVQ+
Sbjct: 410 DIHSRLEGPIAWDVLFNFEQRWRKQGGKDLLIQLRELEDVIIPPSPVMYPDDFEAWNVQL 469

Query: 461 FRSIDHLSASQM--------------FRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 520
           FRSID  +A                  ++  I+R+I +AY+ AIRRA+ FIYIENQYF+G
Sbjct: 470 FRSIDGGAAFGFPETPEDAPEAGLVSGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLG 529

Query: 521 GCHFWDRD----QHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPECESVED 580
               W  D    +     +LIP E++LK+ +KI A ERF VYIV+PMWPEG PE  SV+ 
Sbjct: 530 SSFGWSPDGIKPEDINALHLIPKELSLKILSKIAAGERFTVYIVVPMWPEGIPESASVQA 589

Query: 581 MLHWTRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQ 640
           +L W ++TM MMY+ I +A++  G    PR+YL FFCL NRE ++  ++ P   P+  T 
Sbjct: 590 ILDWQKRTMEMMYKDIVQALKANGIIEDPRNYLTFFCLGNREVKKSGEYEPAEKPEPDTD 649

Query: 641 YGNAQQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEEL 700
           Y  AQ+ RRFMIYVH+K+MIVDD YI+IGSAN+NQRSMDG RD+EIAMG YQ  +     
Sbjct: 650 YIRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGAYQPHHLSTRQ 709

Query: 701 PNGRDISTFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVH 760
           P    I  FR+SLWYEH G  +E FLNPES +CV +V  + +K W +YS E +E     H
Sbjct: 710 PARGQIHGFRMSLWYEHLGMLDESFLNPESEECVRKVNQMAEKYWDLYSSETLEHDLPGH 769

Query: 761 MVTYPVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 762
           ++ YP+ V  +G + +L      FPDTK  + G +S  LPPI TT
Sbjct: 770 LLRYPIGVASEGDVTEL-PGTEFFPDTKARVLGAKSDYLPPILTT 808

[Close]
BLAST of CSPI03G41500 vs. ExPASy TrEMBL
Match: A0A5D3BW81 (Phospholipase D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold863G001380 PE=3 SV=1)

HSP 1 Score: 1557.0 bits (4030), Expect = 0.0e+00
Identity = 738/761 (96.98%), Postives = 748/761 (98.29%), Query Frame = 0 

Query: 1   MAMEVKQKFFHGTLEVTVFHATAYAPSSPLDCLFAGGKCSYVTIKIDNKEVAQTSHEQDR 60
           MAMEVKQKFFHG LEVTVFHATAY P SPLDCLFAGGK SYVTIKIDNKEVAQTSHE DR
Sbjct: 1   MAMEVKQKFFHGMLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEHDR 60

Query: 61  VWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120
           VWNQTFRVLCA+PLTST+TITLKT+RSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS
Sbjct: 61  VWNQTFRVLCAYPLTSTITITLKTARSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120

Query: 121 PELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180
           PELKLRFMLWFKPAVYELSWKKML NGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP
Sbjct: 121 PELKLRFMLWFKPAVYELSWKKMLANGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180

Query: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
           FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ
Sbjct: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240

Query: 241 KADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
           KA+EGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF
Sbjct: 241 KAEEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300

Query: 301 SHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360
           SHHQK IIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS
Sbjct: 301 SHHQKIIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360

Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
           ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420

Query: 421 ESNTNPQKNWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQK+WNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480

Query: 481 GCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPECESVEDMLHW 540
           GCHFWDRD+HCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPE ESVEDMLHW
Sbjct: 481 GCHFWDRDRHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHW 540

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYGNA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQY NA
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNA 600

Query: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGR 660
           QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIA+GCYQIEN+GEELPNGR
Sbjct: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQIENDGEELPNGR 660

Query: 661 DISTFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTY 720
           DIS FRLSLWYEHT GFEEVFLNPESLKCV+RVRSIGDKSWKIYSGEEVEDMKGVH+VTY
Sbjct: 661 DISRFRLSLWYEHTRGFEEVFLNPESLKCVQRVRSIGDKSWKIYSGEEVEDMKGVHLVTY 720

Query: 721 PVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 762
           PVKVKEDG MEDLEENGGHFPDTKC IKGRRSMMLPPIFTT
Sbjct: 721 PVKVKEDGTMEDLEENGGHFPDTKCAIKGRRSMMLPPIFTT 761

[Close]
BLAST of CSPI03G41500 vs. ExPASy TrEMBL
Match: A0A1S3BH90 (Phospholipase D OS=Cucumis melo OX=3656 GN=LOC103490014 PE=3 SV=1)

HSP 1 Score: 1557.0 bits (4030), Expect = 0.0e+00
Identity = 738/761 (96.98%), Postives = 748/761 (98.29%), Query Frame = 0 

Query: 1   MAMEVKQKFFHGTLEVTVFHATAYAPSSPLDCLFAGGKCSYVTIKIDNKEVAQTSHEQDR 60
           MAMEVKQKFFHG LEVTVFHATAY P SPLDCLFAGGK SYVTIKIDNKEVAQTSHE DR
Sbjct: 1   MAMEVKQKFFHGMLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEHDR 60

Query: 61  VWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120
           VWNQTFRVLCA+PLTST+TITLKT+RSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS
Sbjct: 61  VWNQTFRVLCAYPLTSTITITLKTARSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120

Query: 121 PELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180
           PELKLRFMLWFKPAVYELSWKKML NGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP
Sbjct: 121 PELKLRFMLWFKPAVYELSWKKMLANGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180

Query: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
           FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ
Sbjct: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240

Query: 241 KADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
           KA+EGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF
Sbjct: 241 KAEEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300

Query: 301 SHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360
           SHHQK IIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS
Sbjct: 301 SHHQKIIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360

Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
           ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420

Query: 421 ESNTNPQKNWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQK+WNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480

Query: 481 GCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPECESVEDMLHW 540
           GCHFWDRD+HCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPE ESVEDMLHW
Sbjct: 481 GCHFWDRDRHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHW 540

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYGNA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQY NA
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNA 600

Query: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGR 660
           QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIA+GCYQIEN+GEELPNGR
Sbjct: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQIENDGEELPNGR 660

Query: 661 DISTFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTY 720
           DIS FRLSLWYEHT GFEEVFLNPESLKCV+RVRSIGDKSWKIYSGEEVEDMKGVH+VTY
Sbjct: 661 DISRFRLSLWYEHTRGFEEVFLNPESLKCVQRVRSIGDKSWKIYSGEEVEDMKGVHLVTY 720

Query: 721 PVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 762
           PVKVKEDG MEDLEENGGHFPDTKC IKGRRSMMLPPIFTT
Sbjct: 721 PVKVKEDGTMEDLEENGGHFPDTKCAIKGRRSMMLPPIFTT 761

[Close]
BLAST of CSPI03G41500 vs. ExPASy TrEMBL
Match: A0A0A0LGB5 (Phospholipase D OS=Cucumis sativus OX=3659 GN=Csa_3G848850 PE=3 SV=1)

HSP 1 Score: 1551.6 bits (4016), Expect = 0.0e+00
Identity = 741/761 (97.37%), Postives = 742/761 (97.50%), Query Frame = 0 

Query: 1   MAMEVKQKFFHGTLEVTVFHATAYAPSSPLDCLFAGGKCSYVTIKIDNKEVAQTSHEQDR 60
           MAMEVKQKFFHGTLE                CLFAGGKCSYVTIKIDNKEVAQTSHEQDR
Sbjct: 1   MAMEVKQKFFHGTLE----------------CLFAGGKCSYVTIKIDNKEVAQTSHEQDR 60

Query: 61  VWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120
           VWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS
Sbjct: 61  VWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120

Query: 121 PELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180
           PELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP
Sbjct: 121 PELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180

Query: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
           FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ
Sbjct: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240

Query: 241 KADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
           KADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF
Sbjct: 241 KADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300

Query: 301 SHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360
           SHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS
Sbjct: 301 SHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360

Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
           ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420

Query: 421 ESNTNPQKNWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQK+WNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480

Query: 481 GCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPECESVEDMLHW 540
           GCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPE ESVEDMLHW
Sbjct: 481 GCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHW 540

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYGNA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQY NA
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNA 600

Query: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGR 660
           QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGR
Sbjct: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGR 660

Query: 661 DISTFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTY 720
           DIS FRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTY
Sbjct: 661 DISKFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTY 720

Query: 721 PVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 762
           PVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT
Sbjct: 721 PVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 745

[Close]
BLAST of CSPI03G41500 vs. ExPASy TrEMBL
Match: A0A6J1G9V5 (Phospholipase D OS=Cucurbita moschata OX=3662 GN=LOC111452112 PE=3 SV=1)

HSP 1 Score: 1471.1 bits (3807), Expect = 0.0e+00
Identity = 685/761 (90.01%), Postives = 730/761 (95.93%), Query Frame = 0 

Query: 1   MAMEVKQKFFHGTLEVTVFHATAYAPSSPLDCLFAGGKCSYVTIKIDNKEVAQTSHEQDR 60
           M ++ K KFFHGTLEVTVFHAT Y PSSPLDC+F+GGK SYVTIKI+N EVA+T+HE DR
Sbjct: 62  MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 121

Query: 61  VWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120
           VWNQTFRVLCAHPLTST+TIT++T+RSVLG+F IQAQQILKE+SFINGFFPLLMENGKPS
Sbjct: 122 VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 181

Query: 121 PELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180
           PEL+LRFMLWF+PAVYELSWKK+L NGEYKGLRNATFPLRSNCHVTLYQDAHH+ TFQPP
Sbjct: 182 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 241

Query: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
           FHGSS PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD QT IPYALGVKLGELLKQ
Sbjct: 242 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 301

Query: 241 KADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
           KA+EGVAVR++IWDDETSLPIIKNAGIM THDEDARAYF +SKV+CRLCPKLHPMSPPIF
Sbjct: 302 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 361

Query: 301 SHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360
           SHHQKTI VDAQTHINA+NREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 362 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 421

Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
           ISGAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMP L
Sbjct: 422 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 481

Query: 421 ESNTNPQKNWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQK+WNVQVFRSI+HLSASQ+FRN+T+ERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 482 ESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 541

Query: 481 GCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPECESVEDMLHW 540
           GCH WD+DQHCGCTNLIPIEIALKVANKIKARE+FAVY+VIPMWPEG PE ESVEDMLHW
Sbjct: 542 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 601

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYGNA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDF+PPHSPQHAT+Y +A
Sbjct: 602 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 661

Query: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGR 660
           Q HRRFM+YVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIA+GCYQ+EN+G+ELPNGR
Sbjct: 662 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 721

Query: 661 DISTFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTY 720
           DISTFRLSLWYEHT  FEEVFLNPE+L+CVERVRSI D+SWKIYSGEEV DMKGVH+VTY
Sbjct: 722 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 781

Query: 721 PVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 762
           PVKVK+DG +EDLEENGGHFPDTKCPIKGRRSM LPPIFTT
Sbjct: 782 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 822

[Close]
BLAST of CSPI03G41500 vs. ExPASy TrEMBL
Match: A0A6J1KAT1 (Phospholipase D OS=Cucurbita maxima OX=3661 GN=LOC111492613 PE=3 SV=1)

HSP 1 Score: 1464.1 bits (3789), Expect = 0.0e+00
Identity = 680/761 (89.36%), Postives = 728/761 (95.66%), Query Frame = 0 

Query: 1   MAMEVKQKFFHGTLEVTVFHATAYAPSSPLDCLFAGGKCSYVTIKIDNKEVAQTSHEQDR 60
           M ++ +  FFHGTLEVTVFHAT Y PSSPLDC+F+GGK SYVTIKI+N EVA+T+HE DR
Sbjct: 1   MTVQGEHTFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60

Query: 61  VWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120
           VWNQTFRVLCAHPLTST+TIT++T+RSVLG+F IQAQQILKE+SFINGFFPLLMENGKPS
Sbjct: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120

Query: 121 PELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180
           PEL+LRFMLWF+PAVYELSWKK+L NGEYKGLRN TFPLRSNCHVTLYQDAHH+ TFQPP
Sbjct: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQPP 180

Query: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
           FHGSS PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD QTDIPYALGVKLGELLKQ
Sbjct: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQ 240

Query: 241 KADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
           KA+EGVAVR++IWDDETSLP+IKNAGIM THDE+ARAYF +SKV+CRLCPKLHPMSPPIF
Sbjct: 241 KAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLHPMSPPIF 300

Query: 301 SHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360
           SHHQKTI VDAQTHINA+NREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360

Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
           ISGAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMP L
Sbjct: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420

Query: 421 ESNTNPQKNWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQK+WNVQVFRSIDHLSASQ+FRN+T+ERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480

Query: 481 GCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPECESVEDMLHW 540
           GCH WD+DQHCGCTNLIPIEIALKVANKIKARE+FAVY+VIPMWPEG PE ESVEDMLHW
Sbjct: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYGNA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDF+PPHSPQHAT+Y +A
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600

Query: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGR 660
           Q  RRFM+YVHSKVMIVDDLY+LIGSANVNQRSMDGERDTEIA+GCYQ+EN+G+ELPNGR
Sbjct: 601 QHRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660

Query: 661 DISTFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTY 720
           DISTFRLSLWYEHT  FEEVFLNPE+L+CVERVRSI D+SWKIYSGEEV DMKGVH+VTY
Sbjct: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720

Query: 721 PVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 762
           PVKVK+DG +EDLEENGGHFPDTKCPIKGRRSM LPPIFTT
Sbjct: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 761

[Close]
BLAST of CSPI03G41500 vs. NCBI nr
Match: XP_004146826.1 (phospholipase D alpha 4 [Cucumis sativus] >KAE8651293.1 hypothetical protein Csa_001191 [Cucumis sativus])

HSP 1 Score: 1594.3 bits (4127), Expect = 0.0e+00
Identity = 757/761 (99.47%), Postives = 758/761 (99.61%), Query Frame = 0 

Query: 1   MAMEVKQKFFHGTLEVTVFHATAYAPSSPLDCLFAGGKCSYVTIKIDNKEVAQTSHEQDR 60
           MAMEVKQKFFHGTLEVTVFHATAYAPSSPLDCLFAGGKCSYVTIKIDNKEVAQTSHEQDR
Sbjct: 1   MAMEVKQKFFHGTLEVTVFHATAYAPSSPLDCLFAGGKCSYVTIKIDNKEVAQTSHEQDR 60

Query: 61  VWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120
           VWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS
Sbjct: 61  VWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120

Query: 121 PELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180
           PELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP
Sbjct: 121 PELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180

Query: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
           FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ
Sbjct: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240

Query: 241 KADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
           KADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF
Sbjct: 241 KADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300

Query: 301 SHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360
           SHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS
Sbjct: 301 SHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360

Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
           ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420

Query: 421 ESNTNPQKNWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQK+WNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480

Query: 481 GCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPECESVEDMLHW 540
           GCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPE ESVEDMLHW
Sbjct: 481 GCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHW 540

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYGNA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQY NA
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNA 600

Query: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGR 660
           QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGR
Sbjct: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGR 660

Query: 661 DISTFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTY 720
           DIS FRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTY
Sbjct: 661 DISKFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTY 720

Query: 721 PVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 762
           PVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT
Sbjct: 721 PVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 761

[Close]
BLAST of CSPI03G41500 vs. NCBI nr
Match: XP_008447602.1 (PREDICTED: phospholipase D alpha 4 [Cucumis melo] >KAA0051141.1 phospholipase D alpha 4 [Cucumis melo var. makuwa] >TYK03757.1 phospholipase D alpha 4 [Cucumis melo var. makuwa])

HSP 1 Score: 1557.0 bits (4030), Expect = 0.0e+00
Identity = 738/761 (96.98%), Postives = 748/761 (98.29%), Query Frame = 0 

Query: 1   MAMEVKQKFFHGTLEVTVFHATAYAPSSPLDCLFAGGKCSYVTIKIDNKEVAQTSHEQDR 60
           MAMEVKQKFFHG LEVTVFHATAY P SPLDCLFAGGK SYVTIKIDNKEVAQTSHE DR
Sbjct: 1   MAMEVKQKFFHGMLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEHDR 60

Query: 61  VWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120
           VWNQTFRVLCA+PLTST+TITLKT+RSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS
Sbjct: 61  VWNQTFRVLCAYPLTSTITITLKTARSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120

Query: 121 PELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180
           PELKLRFMLWFKPAVYELSWKKML NGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP
Sbjct: 121 PELKLRFMLWFKPAVYELSWKKMLANGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180

Query: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
           FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ
Sbjct: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240

Query: 241 KADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
           KA+EGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF
Sbjct: 241 KAEEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300

Query: 301 SHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360
           SHHQK IIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS
Sbjct: 301 SHHQKIIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360

Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
           ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420

Query: 421 ESNTNPQKNWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQK+WNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480

Query: 481 GCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPECESVEDMLHW 540
           GCHFWDRD+HCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPE ESVEDMLHW
Sbjct: 481 GCHFWDRDRHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHW 540

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYGNA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQY NA
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNA 600

Query: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGR 660
           QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIA+GCYQIEN+GEELPNGR
Sbjct: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQIENDGEELPNGR 660

Query: 661 DISTFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTY 720
           DIS FRLSLWYEHT GFEEVFLNPESLKCV+RVRSIGDKSWKIYSGEEVEDMKGVH+VTY
Sbjct: 661 DISRFRLSLWYEHTRGFEEVFLNPESLKCVQRVRSIGDKSWKIYSGEEVEDMKGVHLVTY 720

Query: 721 PVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 762
           PVKVKEDG MEDLEENGGHFPDTKC IKGRRSMMLPPIFTT
Sbjct: 721 PVKVKEDGTMEDLEENGGHFPDTKCAIKGRRSMMLPPIFTT 761

[Close]
BLAST of CSPI03G41500 vs. NCBI nr
Match: XP_038879261.1 (phospholipase D alpha 4 [Benincasa hispida])

HSP 1 Score: 1531.9 bits (3965), Expect = 0.0e+00
Identity = 722/761 (94.88%), Postives = 740/761 (97.24%), Query Frame = 0 

Query: 1   MAMEVKQKFFHGTLEVTVFHATAYAPSSPLDCLFAGGKCSYVTIKIDNKEVAQTSHEQDR 60
           MAME KQKFFHGTLEVTVFHATAY P SPLDC+F+GGK SYVTIKIDNKEVAQTSHEQDR
Sbjct: 1   MAMEGKQKFFHGTLEVTVFHATAYTPPSPLDCIFSGGKRSYVTIKIDNKEVAQTSHEQDR 60

Query: 61  VWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120
           VWNQTFRVLCAHPLTSTVTIT+KTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS
Sbjct: 61  VWNQTFRVLCAHPLTSTVTITMKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120

Query: 121 PELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180
           PELKLRFMLWFKPA+YELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP
Sbjct: 121 PELKLRFMLWFKPAIYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180

Query: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
           FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDS TDIPYALGVKLGELLKQ
Sbjct: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSHTDIPYALGVKLGELLKQ 240

Query: 241 KADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
           KA+EGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF
Sbjct: 241 KAEEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300

Query: 301 SHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360
           SHHQKTI VDAQTHINA+NREIMSFIGGLDLCDGRYDTEQHSLFHTLN ESHC DFYQTS
Sbjct: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNRESHCSDFYQTS 360

Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
           ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420

Query: 421 ESNTNPQKNWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ES+TNPQK+WNVQVFRSIDHLSASQ+FRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESSTNPQKDWNVQVFRSIDHLSASQVFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480

Query: 481 GCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPECESVEDMLHW 540
           GCH WD+DQHCGCTNLIPIEIALKV NKIKARERF VYIVIPMWPEG PE ESVEDMLHW
Sbjct: 481 GCHLWDKDQHCGCTNLIPIEIALKVVNKIKARERFTVYIVIPMWPEGPPESESVEDMLHW 540

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYGNA 600
           TRQTM MMYRLIGEAIQETGEKAHPRDYLNFFCLANREEE KWDFIPP SPQHATQY N+
Sbjct: 541 TRQTMAMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEEGKWDFIPPQSPQHATQYWNS 600

Query: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGR 660
           QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDG+RDTEIA+GCYQ+EN+GEE PNGR
Sbjct: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGDRDTEIAIGCYQLENDGEEFPNGR 660

Query: 661 DISTFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTY 720
           DISTFRLSLWYEHT  FEEVFLNPESLKCV+RVRSIGDKSW+IYSGEEVEDMKGVH+VTY
Sbjct: 661 DISTFRLSLWYEHTQRFEEVFLNPESLKCVQRVRSIGDKSWEIYSGEEVEDMKGVHLVTY 720

Query: 721 PVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 762
           PVKVKEDG MEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT
Sbjct: 721 PVKVKEDGSMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 761

[Close]
BLAST of CSPI03G41500 vs. NCBI nr
Match: XP_023525777.1 (phospholipase D alpha 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1474.1 bits (3815), Expect = 0.0e+00
Identity = 686/761 (90.14%), Postives = 730/761 (95.93%), Query Frame = 0 

Query: 1   MAMEVKQKFFHGTLEVTVFHATAYAPSSPLDCLFAGGKCSYVTIKIDNKEVAQTSHEQDR 60
           M +  K KFFHGTLEVTVFHAT Y PSSPLDC+F+GGK SYVTIKI+N EVA+T+HE DR
Sbjct: 61  MTVHGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 120

Query: 61  VWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120
           VWNQTFRVLCAHPLTST+TIT++TSRSVLG+F IQAQQILKE+SFINGFFPLLMENGKPS
Sbjct: 121 VWNQTFRVLCAHPLTSTITITMRTSRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 180

Query: 121 PELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180
           PEL+LRFMLWF+PAVYELSWKK+L NGEYKGLRNATFPLRSNCHVTLYQDAHH+ TFQPP
Sbjct: 181 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 240

Query: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
           FHGSS PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD QTDIPYALGVKLGELLKQ
Sbjct: 241 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQ 300

Query: 241 KADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
           KA+EGVAVR++IWDDETSLP+IKNAGIM THDEDARAYF +SKV+CRLCPKLHPMSPPIF
Sbjct: 301 KAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 360

Query: 301 SHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360
           SHHQKTI VDAQTHINA+NREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 361 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 420

Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
           ISGAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMP L
Sbjct: 421 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 480

Query: 421 ESNTNPQKNWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQK+WNVQVFRSIDHLSASQ+FRN+T+ERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 481 ESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 540

Query: 481 GCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPECESVEDMLHW 540
           GCH WD+DQHCGCTNLIPIEIALKVA KI+ARE+FAVY+VIPMWPEG PE ESVEDMLHW
Sbjct: 541 GCHLWDKDQHCGCTNLIPIEIALKVATKIRAREKFAVYVVIPMWPEGPPESESVEDMLHW 600

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYGNA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDF+PPHSPQHAT+Y +A
Sbjct: 601 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 660

Query: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGR 660
           QQHRRFM+YVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIA+GCYQ+EN+G+ELPNGR
Sbjct: 661 QQHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 720

Query: 661 DISTFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTY 720
           DISTFRLSLWYEHT  FEEVFLNPE+L+CVERVRSI D+SWKIYSGEEV DMKGVH+VTY
Sbjct: 721 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 780

Query: 721 PVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 762
           PVKVK+DG +EDLEENGGHFPDTKCPIKGRRSM LPPIFTT
Sbjct: 781 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 821

[Close]
BLAST of CSPI03G41500 vs. NCBI nr
Match: KAG6606722.1 (Phospholipase D alpha 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1473.0 bits (3812), Expect = 0.0e+00
Identity = 685/761 (90.01%), Postives = 730/761 (95.93%), Query Frame = 0 

Query: 1   MAMEVKQKFFHGTLEVTVFHATAYAPSSPLDCLFAGGKCSYVTIKIDNKEVAQTSHEQDR 60
           M ++ K KFFHGTLEVTVFHAT Y PSSPLDC+F+GGK SYVTIKI+N EVA+T+HE DR
Sbjct: 61  MTLQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 120

Query: 61  VWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120
           VWNQTFRVLCAHPLTST+TIT++T+RSVLG+F IQAQQILKE+SFINGFFPLLMENGKPS
Sbjct: 121 VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 180

Query: 121 PELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180
           PEL+LRFMLWF+PAVYELSWKK+L NGEYKGLRNATFPLRSNCHVTLYQDAHH+ TFQPP
Sbjct: 181 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 240

Query: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
           FHGSS PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD QT IPYALGVKLGELLKQ
Sbjct: 241 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 300

Query: 241 KADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
           KA+EGVAVR++IWDDETSLPIIKNAGIM THDEDARAYF +SKV+CRLCPKLHPMSPPIF
Sbjct: 301 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 360

Query: 301 SHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360
           SHHQKTI VDAQTHINA+NREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 361 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 420

Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
           ISGAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMP L
Sbjct: 421 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 480

Query: 421 ESNTNPQKNWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQK+WNVQVFRSIDHLSASQ+FRN+T+ERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 481 ESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 540

Query: 481 GCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPECESVEDMLHW 540
           GCH WD+DQHCGCTNLIPIEIALKVANKIKARE+FAVY+VIPMWPEG PE ESVEDMLHW
Sbjct: 541 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 600

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYGNA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDF+PPHSPQHAT+Y +A
Sbjct: 601 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 660

Query: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGR 660
           Q HRRFM+YVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIA+GCYQ+EN+G+ELPNGR
Sbjct: 661 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 720

Query: 661 DISTFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTY 720
           DISTFRLSLWYEHT  FEEVFLNPE+L+CVER+RSI D+SWKIYSGEEV DMKGVH+VTY
Sbjct: 721 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERIRSIADESWKIYSGEEVADMKGVHLVTY 780

Query: 721 PVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 762
           PVKVK+DG +EDLEENGGHFPDTKCPIKGRRSM LPPIFTT
Sbjct: 781 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 821

[Close]
BLAST of CSPI03G41500 vs. TAIR 10
Match: AT1G55180.1 (phospholipase D alpha 4 )

HSP 1 Score: 971.1 bits (2509), Expect = 5.3e-283
Identity = 460/776 (59.28%), Postives = 593/776 (76.42%), Query Frame = 0 

Query: 1   MAMEVKQKFFHGTLEVTVFHATAYAPSSPLDCLFAGGKCSYVTIKIDNKEVAQTSHEQDR 60
           M +E ++K+FHGTLE+T+F AT ++P  P +C+    K +YVTIKI+ K+VA+TS E DR
Sbjct: 1   MELEEQKKYFHGTLEITIFDATPFSPPFPFNCICTKPKAAYVTIKINKKKVAKTSSEYDR 60

Query: 61  VWNQTFRVLCAHPLT-STVTITLKTSRSVLGKFYIQAQQIL-KEASFINGFFPLLMENGK 120
           +WNQTF++LCAHP+T +T+TITLKT  SVLG+F I A+QIL   ++ INGFFPL+ +NG 
Sbjct: 61  IWNQTFQILCAHPVTDTTITITLKTRCSVLGRFRISAEQILTSNSAVINGFFPLIADNGS 120

Query: 121 PSPELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQ 180
               LKL+ ++WF+PA  E  W + L    ++G+RNA+FP RSNC V LYQDAHH  TF 
Sbjct: 121 TKRNLKLKCLMWFRPAYLEPGWCRALEEASFQGIRNASFPQRSNCRVVLYQDAHHKATFD 180

Query: 181 P-----PFHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVK 240
           P     PF+     R LWEDVYKAI++A+HLVYIAGW+ NP +VLVRD++T+IP+A+GV 
Sbjct: 181 PRVDDVPFNA----RNLWEDVYKAIESARHLVYIAGWALNPNLVLVRDNETEIPHAVGVT 240

Query: 241 LGELLKQKADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLH 300
           +GELLK+K++EGVAVR+++W+DETSLP+IKN G+M T+ E A AYF ++ V+CRLCP+LH
Sbjct: 241 VGELLKRKSEEGVAVRVMLWNDETSLPMIKNKGVMRTNVERALAYFRNTNVVCRLCPRLH 300

Query: 301 PMSPPIFSHHQKTIIVDAQ-THINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESH 360
              P  F+HHQKTI +D + T+ + + REIMSF+GG DLCDGRYDTE+HSLF TL TE+ 
Sbjct: 301 KKLPTAFAHHQKTITLDTRVTNSSTKEREIMSFLGGFDLCDGRYDTEEHSLFRTLGTEA- 360

Query: 361 CRDFYQTSISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSI 420
             DFYQTS++GAKL +GGPREPWHD H  V G AAWD+L NFEQRWTKQ + S+LV TS 
Sbjct: 361 --DFYQTSVAGAKLSRGGPREPWHDCHVSVVGGAAWDVLKNFEQRWTKQCNPSVLVNTSG 420

Query: 421 LLKLMPQLESNTNPQKNWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIY 480
           +  L+          + WNVQV RSIDH+SA++M R + +E+++H+ YV AIR+AERFIY
Sbjct: 421 IRNLVNLTGPTEENNRKWNVQVLRSIDHISATEMPRGLPVEKSVHDGYVAAIRKAERFIY 480

Query: 481 IENQYFIGGCHFWD--RDQHC-GCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSP 540
           IENQYF+G C  W+   D+ C GCTNLIP+EIALK+A KI+ARERFAVYIVIPMWPEG P
Sbjct: 481 IENQYFMGSCDHWESKNDKICSGCTNLIPVEIALKIAAKIRARERFAVYIVIPMWPEGPP 540

Query: 541 ECESVEDMLHWTRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPH 600
           E E+VE++LHWTR+TM+MMY++IGEAI E G+K+HPRDYLNFFCLANREE+R  +F    
Sbjct: 541 ESETVEEILHWTRETMSMMYQIIGEAIWEVGDKSHPRDYLNFFCLANREEKRDGEFEAVS 600

Query: 601 SPQHATQYGNAQQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQI 660
           SP   T Y NAQ++RRFM+YVHSK+MIVDD YILIGSAN+NQRSMDG RDTEIA+GCYQ 
Sbjct: 601 SPHQKTHYWNAQRNRRFMVYVHSKLMIVDDTYILIGSANINQRSMDGCRDTEIAIGCYQT 660

Query: 661 ENEGEELPNGRDISTFRLSLWYEHTGG----FEEVFLNPESLKCVERVRSIGDKSWKIYS 720
                   N  +I  +RLSLWYEHTGG     +     PESL+CV  +R+IG++ W+IYS
Sbjct: 661 NTN-----NTNEIQAYRLSLWYEHTGGKITADDLSSSEPESLECVRGLRTIGEQMWEIYS 720

Query: 721 GEEVEDMKGVHMVTYPVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 762
           G++V DM G+H+V YP+ V  DG +E++ +  G FPDTK  +KG+RS M PP+ TT
Sbjct: 721 GDKVVDMLGIHLVAYPISVTGDGAVEEVGD--GCFPDTKTLVKGKRSKMFPPVLTT 762

[Close]
BLAST of CSPI03G41500 vs. TAIR 10
Match: AT3G15730.1 (phospholipase D alpha 1 )

HSP 1 Score: 607.1 bits (1564), Expect = 2.0e-173
Identity = 331/814 (40.66%), Postives = 467/814 (57.37%), Query Frame = 0 

Query: 7   QKFFHGTLEVTVFHATA--------------YAPSSPLDCLFAGGKCSYVTIKIDNKEVA 66
           Q   HGTL  T++   A               A       +  G    Y TI +    V 
Sbjct: 3   QHLLHGTLHATIYEVDALHGGGVRQGFLGKILANVEETIGVGKGETQLYATIDLQKARVG 62

Query: 67  QT----SHEQDRVWNQTFRVLCAHPLTSTVTITLKTSR----SVLGKFYIQAQQILKEAS 126
           +T    +  ++  W ++F + CAH L S +  T+K       +++G+ YI   Q++    
Sbjct: 63  RTRKIKNEPKNPKWYESFHIYCAH-LASDIIFTVKDDNPIGATLIGRAYIPVDQVI-NGE 122

Query: 127 FINGFFPLLMENGKP-SPELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNC 186
            ++ +  +L  +  P     K+   L +     + +W   + + ++ G+    F  R  C
Sbjct: 123 EVDQWVEILDNDRNPIQGGSKIHVKLQYFHVEEDRNWNMGIKSAKFPGVPYTFFSQRQGC 182

Query: 187 HVTLYQDAHHLPTFQP--PFHGSST--PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLV 246
            V+LYQDAH    F P  P  G     P+R WED++ AI NAKHL+YI GWS   ++ LV
Sbjct: 183 KVSLYQDAHIPDNFVPRIPLAGGKNYEPQRCWEDIFDAISNAKHLIYITGWSVYAEIALV 242

Query: 247 RDSQTDIPYALGVKLGELLKQKADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYF 306
           RDS+   P    V +GELLK+KA EGV V +L+WDD TS+ ++K  G+M THDE+   +F
Sbjct: 243 RDSRRPKPGG-DVTIGELLKKKASEGVRVLLLVWDDRTSVDVLKKDGLMATHDEETENFF 302

Query: 307 LHSKVICRLCPKLHP----------MSPPIFSHHQKTIIVDAQ--THINAQNREIMSFIG 366
             S V C LCP+ +P              +F+HHQK ++VD++  +   ++ R I+SF+G
Sbjct: 303 RGSDVHCILCPR-NPDDGGSIVQSLQISTMFTHHQKIVVVDSEMPSRGGSEMRRIVSFVG 362

Query: 367 GLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTSISGAKLQKGGPREPWHDVHACVTGEAA 426
           G+DLCDGRYDT  HSLF TL+T  H  DF+Q + +GA + KGGPREPWHD+H+ + G  A
Sbjct: 363 GIDLCDGRYDTPFHSLFRTLDTVHH-DDFHQPNFTGAAITKGGPREPWHDIHSRLEGPIA 422

Query: 427 WDILTNFEQRWTKQSDASLLVPTSIL--LKLMPQLESNTNPQKNWNVQVFRSIDHLSASQ 486
           WD++ NFEQRW+KQ    +LV    L  + + P           WNVQ+FRSID  +A+ 
Sbjct: 423 WDVMYNFEQRWSKQGGKDILVKLRDLSDIIITPSPVMFQEDHDVWNVQLFRSIDGGAAAG 482

Query: 487 M--------------FRNMTIERTIHEAYVEAIRRAERFIYIENQYFIGGCHFWDRD--- 546
                           ++  I+R+I +AY+ AIRRA+ FIY+ENQYF+G    W  D   
Sbjct: 483 FPESPEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRAKDFIYVENQYFLGSSFAWAADGIT 542

Query: 547 -QHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPECESVEDMLHWTRQTMTM 606
            +     +LIP E++LK+ +KI+  E+F VY+V+PMWPEG PE  SV+ +L W R+TM M
Sbjct: 543 PEDINALHLIPKELSLKIVSKIEKGEKFRVYVVVPMWPEGLPESGSVQAILDWQRRTMEM 602

Query: 607 MYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYGNAQQHRRFM 666
           MY+ + +A++  G +  PR+YL FFCL NRE ++  ++ P   P   T Y  AQ+ RRFM
Sbjct: 603 MYKDVIQALRAQGLEEDPRNYLTFFCLGNREVKKDGEYEPAEKPDPDTDYMRAQEARRFM 662

Query: 667 IYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGRDISTFRL 726
           IYVH+K+MIVDD YI+IGSAN+NQRSMDG RD+EIAMG YQ  +     P    I  FR+
Sbjct: 663 IYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPHHLSHRQPARGQIHGFRM 722

Query: 727 SLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTYPVKVKED 762
           SLWYEH G  +E FL+P SL+C+E+V  I DK W  YS E +E     H++ YP+ V  +
Sbjct: 723 SLWYEHLGMLDETFLDPSSLECIEKVNRISDKYWDFYSSESLEHDLPGHLLRYPIGVASE 782

[Close]
BLAST of CSPI03G41500 vs. TAIR 10
Match: AT5G25370.1 (phospholipase D alpha 3 )

HSP 1 Score: 605.1 bits (1559), Expect = 7.6e-173
Identity = 336/778 (43.19%), Postives = 461/778 (59.25%), Query Frame = 0 

Query: 32  CLFAGGKCSYVTIKIDNKEVAQTSHEQDRVWNQTFRVLCAHPLTSTVTITLK----TSRS 91
           C    G   Y TI +D   VA+T   +   W Q+F V  AH + S +  T+K     S S
Sbjct: 53  CTSLFGGHLYATIDLDRSRVARTMMRRHPKWLQSFHVYTAHSI-SKIIFTVKEDEPVSAS 112

Query: 92  VLGKFYIQAQQILKEASFINGFFPLLMENGKP-SPELKLRFMLWFKPAVYELSWKKMLGN 151
           ++G+ Y+   +++     I+ +  +L EN +P     KL   + F     +++W K +  
Sbjct: 113 LIGRAYLPVTEVI-TGQPIDRWLDILDENRRPIQGGSKLHVRVKFTHVTQDVNWNKGIIL 172

Query: 152 GEYKGLRNATFPLRSNCHVTLYQDAHHLPTF-QPPFHGSST---PRRLWEDVYKAIDNAK 211
             + G+ NA F  R  C VTLYQDAH L  +      G        R WE+++ AI  AK
Sbjct: 173 PSFNGVPNAYFNQREGCKVTLYQDAHVLNEYPDVTLTGGQVIYKHHRCWEEIFDAIWEAK 232

Query: 212 HLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKADEGVAVRILIWDDETSLPII 271
           HL+YIAGWS N  + LVRD +   P    +KLGELLK+KA+E V V +L+WDD TS  + 
Sbjct: 233 HLIYIAGWSVNTDVTLVRDPKRTRPGG-DLKLGELLKKKAEENVTVLMLVWDDRTSHEVF 292

Query: 272 KNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPI---------FSHHQKTIIVDAQT 331
           K  G+M THD++   YF ++KV C LCP+       I         F+HHQKTI+VD++ 
Sbjct: 293 KRDGLMMTHDQETYDYFKNTKVRCVLCPRNPDNGDSIVQGFEVATMFTHHQKTIVVDSEV 352

Query: 332 HINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTSISGAKLQKGGPRE 391
             +   R I+SF+GG+DLCDGRYDT +H LF TLN+  H  DF+Q +  GA ++KGGPRE
Sbjct: 353 DGSLTKRRIVSFLGGIDLCDGRYDTVEHPLFGTLNS-VHANDFHQPNFDGASIKKGGPRE 412

Query: 392 PWHDVHACVTGEAAWDILTNFEQRWTKQSDA-----SLLVPTSILLKLMPQLESNTNPQK 451
           PWHD+H  + G AAWD+L NFEQRW KQ        S+     I +  +P ++ +   ++
Sbjct: 413 PWHDIHCKLDGPAAWDVLYNFEQRWMKQGSGRRYLISMAQLAEITVPPLPIVQPDN--EE 472

Query: 452 NWNVQVFRSIDHLSASQMF---------------RNMTIERTIHEAYVEAIRRAERFIYI 511
            W VQVFRSID   A + F               ++  IER+I +AYV AIRRA+ FIYI
Sbjct: 473 GWTVQVFRSIDD-GAVEGFPEDPREAASIGLISGKDNVIERSIQDAYVNAIRRAKNFIYI 532

Query: 512 ENQYFIGGCHFWD-RD---QHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSP 571
           ENQYF+G    W+ RD          LIP EI+LK+ +KI+A ERF+VYIVIP+WPEG P
Sbjct: 533 ENQYFLGSSFGWNSRDINLNEINALQLIPKEISLKIVSKIEAGERFSVYIVIPLWPEGKP 592

Query: 572 ECESVEDMLHWTRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPH 631
              SV+ +L W R+TM MMY  I  A+++ G  A+PRDYL FFCL NRE+ +  +++PP 
Sbjct: 593 GSASVQAILDWQRRTMEMMYTDIIIALRKKGLDANPRDYLTFFCLGNREKGKVGEYLPPE 652

Query: 632 SPQHATQYGNAQQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQ- 691
            P+  + Y  AQ+ RRFMIYVHSK+MIVDD YI+IGSAN+NQRSMDG RDTEIAMG YQ 
Sbjct: 653 KPEANSDYARAQESRRFMIYVHSKMMIVDDEYIIIGSANINQRSMDGGRDTEIAMGAYQP 712

Query: 692 --IENEGEELPNGRDISTFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSG 751
             + +     P G+ I +FR+SLW EH       F  PES +C+  V +  D+ W +YS 
Sbjct: 713 SHLLSTNNMRPVGQ-IFSFRISLWLEHLRVTTNAFQCPESEECIRMVNATADELWGLYSA 772

Query: 752 EEV---EDMKGVHMVTYPVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 762
           +E    +D+ G H+++YP+ +  +G + +L      FPDT   + G +S  LPPI T+
Sbjct: 773 QEYPRNDDLPG-HLLSYPISIGSNGEVTNL-AGTEFFPDTNAKVVGEKSNYLPPILTS 820

[Close]
BLAST of CSPI03G41500 vs. TAIR 10
Match: AT1G52570.1 (phospholipase D alpha 2 )

HSP 1 Score: 599.4 bits (1544), Expect = 4.2e-171
Identity = 323/767 (42.11%), Postives = 456/767 (59.45%), Query Frame = 0 

Query: 41  YVTIKIDNKEVAQ----TSHEQDRVWNQTFRVLCAHPLTSTVTITLKTSR----SVLGKF 100
           Y TI ++   V +    T   ++  W ++F + C H +   V  T+K +     +++G+ 
Sbjct: 51  YATIDLEKARVGRTRKITKEPKNPKWFESFHIYCGH-MAKHVIFTVKDANPIGATLIGRG 110

Query: 101 YIQAQQILKEASFINGFFPLLMENGKPSPELKLRFMLWFKPAVYELSWKKMLGNGEYKGL 160
           YI  + IL           L  E    +   K+   L +     + +W + + + ++ G+
Sbjct: 111 YIPVEDILHGEEVDRWVDILDNEKNPIAGGSKIHVKLQYFGVEKDKNWNRGIKSAKFPGV 170

Query: 161 RNATFPLRSNCHVTLYQDAHHLPTFQP--PFHGSST--PRRLWEDVYKAIDNAKHLVYIA 220
               F  R  C V+LYQDAH    F P  P  G     P R WED++ AI NAKHL+YI 
Sbjct: 171 PYTFFSQRRGCKVSLYQDAHIPGNFVPKIPLAGGKNYEPHRCWEDIFDAITNAKHLIYIT 230

Query: 221 GWSFNPKMVLVRDSQTDIPYALG-VKLGELLKQKADEGVAVRILIWDDETSLPIIKNAGI 280
           GWS   ++ LVRDS+   P   G V +GELLK+KA EGV V +L+WDD TS+ ++K  G+
Sbjct: 231 GWSVYTEISLVRDSRR--PKQGGDVTVGELLKKKASEGVKVILLVWDDRTSVDLLKKDGL 290

Query: 281 MNTHDEDARAYFLHSKVICRLCPK---------LHPMSPPIFSHHQKTIIVDAQTHI-NA 340
           M THDE+   +F  + V C LCP+          +     +F+HHQK ++VD++     +
Sbjct: 291 MATHDEETENFFRGTDVNCILCPRNPDDGGSIVQNLQISTMFTHHQKIVVVDSEMPSGGS 350

Query: 341 QNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTSISGAKLQKGGPREPWHD 400
           ++R I+SF+GGLDLCDGRYDT  HSLF TL+T +H  DF+Q + +GA + KGGPREPWHD
Sbjct: 351 RSRRIVSFVGGLDLCDGRYDTPFHSLFRTLDT-AHHDDFHQPNFTGAAITKGGPREPWHD 410

Query: 401 VHACVTGEAAWDILTNFEQRWTKQSDASLLVPT----SILLKLMPQLESNTNPQKNWNVQ 460
           +H  + G  AWD+L NFEQRW++Q    +LV       I++   P L S  +    WNVQ
Sbjct: 411 IHCRLEGPIAWDVLYNFEQRWSRQGGKDILVKMRELGDIIIPPSPVLFSEDHDV--WNVQ 470

Query: 461 VFRSIDHLSASQM--------------FRNMTIERTIHEAYVEAIRRAERFIYIENQYFI 520
           +FRSID  +A+                 ++  I+R+I +AY+ AIRRA+ FIYIENQYF+
Sbjct: 471 LFRSIDGGAAAGFPDSPEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRAKDFIYIENQYFL 530

Query: 521 GGCHFWDRD----QHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPECESVE 580
           G    W  D    +     +LIP E++LK+ +KIKA E+F VY+V+PMWPEG PE  SV+
Sbjct: 531 GSSFAWSADGIKPEEINALHLIPKELSLKIVSKIKAGEKFKVYVVVPMWPEGIPESGSVQ 590

Query: 581 DMLHWTRQTMTMMYRLIGEAIQETG-EKAHPRDYLNFFCLANREEERKWDFIPPHSPQHA 640
            +L W ++TM MMY+ + +A++E G E   PRDYL FFCL NRE ++  ++ P   P+  
Sbjct: 591 AILDWQKRTMEMMYKDVIKALRENGLEGEDPRDYLTFFCLGNREVKKDGEYEPSEKPEPD 650

Query: 641 TQYGNAQQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGE 700
           T Y  AQ+ RRFMIYVH+K+MIVDD YI+IGSAN+NQRSMDG RD+EIAMG YQ  +   
Sbjct: 651 TDYIRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPYHLST 710

Query: 701 ELPNGRDISTFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKG 760
             P    I  FR+SLWYEH G  +E FL+P S +C+++V  + DK W +YS E +E    
Sbjct: 711 RQPARGQIHGFRMSLWYEHLGMLDETFLDPSSQECIQKVNRVADKYWDLYSSESLEHDLP 770

Query: 761 VHMVTYPVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 762
            H++ YP+ +  +G + +L      FPDTK  I G +S  +PPI TT
Sbjct: 771 GHLLRYPIGIASEGNITEL-PGCEFFPDTKARILGVKSDYMPPILTT 810

[Close]
BLAST of CSPI03G41500 vs. TAIR 10
Match: AT2G42010.1 (phospholipase D beta 1 )

HSP 1 Score: 541.6 bits (1394), Expect = 1.0e-153
Identity = 323/768 (42.06%), Postives = 442/768 (57.55%), Query Frame = 0 

Query: 41   YVTIKIDNKEVAQT---SHEQDRVWNQTFRVLCAHPLTSTVTITLKTS----RSVLGKFY 100
            YV++ +    + +T   S+ ++ VW Q F V  AH   + V   +K S      ++G   
Sbjct: 319  YVSVSVAGAVIGRTYVMSNSENPVWMQHFYVPVAHH-AAEVHFVVKDSDVVGSQLIGLVT 378

Query: 101  IQAQQILKEASFINGFFPLLMENGKP-SPELKLRFMLWFKPAVYELSWKKMLGNG-EYKG 160
            I  +QI   A  I G +P+L  NGKP  P   L   + + P      +   +G G +Y+G
Sbjct: 379  IPVEQIYSGAK-IEGTYPILNSNGKPCKPGANLSLSIQYTPMDKLSVYHHGVGAGPDYQG 438

Query: 161  LRNATFPLRSNCHVTLYQDAH----HLPTFQPPFHGSSTPRRLWEDVYKAIDNAKHLVYI 220
            +    FPLR    V LYQDAH     LP  +     S    + W D++ AI  A+ L+YI
Sbjct: 439  VPGTYFPLRKGGTVRLYQDAHVPEGMLPGIRLDNGMSYEHGKCWHDMFDAIRQARRLIYI 498

Query: 221  AGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKADEGVAVRILIWDDETSLPII--KNA 280
             GWS   K+ L+RD    +  A    LGELL+ K+ EGV V +LIWDD TS  I+  K  
Sbjct: 499  TGWSVWHKVKLIRDK---LGPASECTLGELLRSKSQEGVRVLLLIWDDPTSRSILGYKTD 558

Query: 281  GIMNTHDEDARAYFLHSKVICRLCP----KLHPMSP-----PIFSHHQKTIIVDAQTHIN 340
            G+M THDE+ R +F HS V   LCP    K H          I++HHQK +IVDA     
Sbjct: 559  GVMATHDEETRRFFKHSSVQVLLCPRNAGKRHSWVKQREVGTIYTHHQKNVIVDADA--G 618

Query: 341  AQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTSISGAKLQKGGPREPWH 400
               R+I++F+GGLDLCDGRYDT QH LF TL T  H  DF+  + +G     G PREPWH
Sbjct: 619  GNRRKIIAFVGGLDLCDGRYDTPQHPLFRTLQT-IHKDDFHNPTFTGN--LSGCPREPWH 678

Query: 401  DVHACVTGEAAWDILTNFEQRWTKQS------------DASLL----VPTSILLKLMPQL 460
            D+H+ + G AA+D+LTNFE+RW K +            D +LL    +P  + +   P +
Sbjct: 679  DLHSKIDGPAAYDVLTNFEERWLKAAKPSGIKKFKTSYDDALLRIDRIPDILGVSDTPTV 738

Query: 461  ESNTNPQKNWNVQVFRSIDHLSASQM--------------FRNMTIERTIHEAYVEAIRR 520
              N +P+  W+VQ+FRSID  S                   +N+ I+ +IH AYV+AIR 
Sbjct: 739  SEN-DPEA-WHVQIFRSIDSNSVKGFPKDPKDATCKNLVCGKNVLIDMSIHTAYVKAIRA 798

Query: 521  AERFIYIENQYFIGGCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPE 580
            A+ FIYIENQYFIG  + W+  +  G  NLIP+EIALK+A KI+A ERFA YIVIPMWPE
Sbjct: 799  AQHFIYIENQYFIGSSYNWNAHKDIGANNLIPMEIALKIAEKIRANERFAAYIVIPMWPE 858

Query: 581  GSPECESVEDMLHWTRQTMTMMYRLIGEAIQETGEKA--HPRDYLNFFCLANREEERKWD 640
            G P   + + +L+W  +T+ MMY  I +A+ ETG +    P+DYLNFFCL NRE     D
Sbjct: 859  GVPTGAATQRILYWQHKTIQMMYETIYKALVETGLEGAFSPQDYLNFFCLGNREMVDGID 918

Query: 641  FIPPHSPQHA-TQYGNAQQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIA 700
                 SP +A T    +++ RRFM+YVHSK M+VDD Y++IGSAN+NQRSM+G RDTEIA
Sbjct: 919  NSGTGSPSNANTPQALSRKSRRFMVYVHSKGMVVDDEYVVIGSANINQRSMEGTRDTEIA 978

Query: 701  MGCYQIENEGEELPNG--RDISTFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSW 750
            MG YQ ++      +G    I  +R+SLW EH    ++ F  PES++CV +VR++G+++W
Sbjct: 979  MGAYQPQHTWARKHSGPRGQIYGYRMSLWAEHMATLDDCFTQPESIECVRKVRTMGERNW 1038

The following BLAST results are available for this feature:
BLAST of CSPI03G41500 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9C8887.4e-28259.28Phospholipase D alpha 4 OS=Arabidopsis thaliana OX=3702 GN=PLDALPHA4 PE=2 SV=1[more]
Q432701.5e-17841.60Phospholipase D alpha 1 OS=Zea mays OX=4577 GN=PLD1 PE=2 SV=1[more]
Q430072.0e-17841.84Phospholipase D alpha 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PLD1 PE=1 SV... [more]
P934003.5e-17542.34Phospholipase D alpha 1 OS=Nicotiana tabacum OX=4097 GN=PLD1 PE=2 SV=2[more]
Q411428.7e-17442.88Phospholipase D alpha 1 OS=Ricinus communis OX=3988 GN=PLD1 PE=1 SV=1[more]
BLAST of CSPI03G41500 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3BW810.0e+0096.98Phospholipase D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold863G0013... [more]
A0A1S3BH900.0e+0096.98Phospholipase D OS=Cucumis melo OX=3656 GN=LOC103490014 PE=3 SV=1[more]
A0A0A0LGB50.0e+0097.37Phospholipase D OS=Cucumis sativus OX=3659 GN=Csa_3G848850 PE=3 SV=1[more]
A0A6J1G9V50.0e+0090.01Phospholipase D OS=Cucurbita moschata OX=3662 GN=LOC111452112 PE=3 SV=1[more]
A0A6J1KAT10.0e+0089.36Phospholipase D OS=Cucurbita maxima OX=3661 GN=LOC111492613 PE=3 SV=1[more]
BLAST of CSPI03G41500 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004146826.10.0e+0099.47phospholipase D alpha 4 [Cucumis sativus] >KAE8651293.1 hypothetical protein Csa... [more]
XP_008447602.10.0e+0096.98PREDICTED: phospholipase D alpha 4 [Cucumis melo] >KAA0051141.1 phospholipase D ... [more]
XP_038879261.10.0e+0094.88phospholipase D alpha 4 [Benincasa hispida][more]
XP_023525777.10.0e+0090.14phospholipase D alpha 4 [Cucurbita pepo subsp. pepo][more]
KAG6606722.10.0e+0090.01Phospholipase D alpha 4, partial [Cucurbita argyrosperma subsp. sororia][more]
BLAST of CSPI03G41500 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G55180.15.3e-28359.28phospholipase D alpha 4 [more]
AT3G15730.12.0e-17340.66phospholipase D alpha 1 [more]
AT5G25370.17.6e-17343.19phospholipase D alpha 3 [more]
AT1G52570.14.2e-17142.11phospholipase D alpha 2 [more]
AT2G42010.11.0e-15342.06phospholipase D beta 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001736Phospholipase D/TransphosphatidylaseSMARTSM00155pld_4coord: 606..633
e-value: 3.6E-6
score: 36.5
coord: 300..335
e-value: 12.0
score: 12.5
IPR001736Phospholipase D/TransphosphatidylasePFAMPF00614PLDccoord: 300..335
e-value: 1.3E-7
score: 31.5
coord: 607..633
e-value: 1.4E-5
score: 25.1
IPR001736Phospholipase D/TransphosphatidylasePROSITEPS50035PLDcoord: 298..335
score: 9.603999
IPR001736Phospholipase D/TransphosphatidylasePROSITEPS50035PLDcoord: 606..633
score: 14.375599
IPR035892C2 domain superfamilyGENE3D2.60.40.150C2 domaincoord: 2..131
e-value: 3.5E-6
score: 28.9
IPR035892C2 domain superfamilySUPERFAMILY49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 6..129
IPR000008C2 domainPFAMPF00168C2coord: 12..111
e-value: 4.4E-7
score: 30.1
IPR024632Phospholipase D, C-terminalPFAMPF12357PLD_Ccoord: 678..750
e-value: 3.7E-23
score: 81.2
NoneNo IPR availableGENE3D3.30.870.10Endonuclease Chain Acoord: 173..432
e-value: 8.9E-26
score: 92.3
NoneNo IPR availableGENE3D3.30.870.10Endonuclease Chain Acoord: 446..652
e-value: 2.4E-11
score: 45.4
NoneNo IPR availablePANTHERPTHR18896:SF137PHOSPHOLIPASE D ALPHA 4coord: 7..761
NoneNo IPR availableSUPERFAMILY56024Phospholipase D/nucleasecoord: 144..409
NoneNo IPR availableSUPERFAMILY56024Phospholipase D/nucleasecoord: 425..690
IPR011402Phospholipase D, plantPIRSFPIRSF036470PLD_plantcoord: 2..761
e-value: 2.3E-273
score: 906.8
IPR015679Phospholipase D familyPANTHERPTHR18896PHOSPHOLIPASE Dcoord: 7..761

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G41500.1CSPI03G41500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046470 phosphatidylcholine metabolic process
biological_process GO:0009395 phospholipid catabolic process
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0070290 N-acylphosphatidylethanolamine-specific phospholipase D activity
molecular_function GO:0004630 phospholipase D activity
molecular_function GO:0003824 catalytic activity