CSPI03G38610 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G38610
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionSacchrp_dh_NADP domain-containing protein
LocationChr3: 33320968 .. 33321267 (+)
RNA-Seq ExpressionCSPI03G38610
SyntenyCSPI03G38610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGCCGAGTATGACGAGAGGGCGGTTGAGAGTGGGAGTTTGGTGGTTTCTGGATGTGGGTTTGATTCGGTTCCAACGAAATTGGGTTTGATATTCAATTTGAGGCAGTGGGTTGGGAAATCAACGCCGAGTTGGGTTGAAGCGTATGTGAATGTGGAGTCTAATGGAGGTATGTCTTATAATTTTGGGACTTACGAATCAACTGTTTTGGATGTGACTAATGTTGATGCGTTGGTTCAATTGAGACAGTCAAGGACCCCTAGACGTGGCTCCAAGGTTAGCAAAATTATTTCTCTC

mRNA sequence

ATGGAGGCCGAGTATGACGAGAGGGCGGTTGAGAGTGGGAGTTTGGTGGTTTCTGGATGTGGGTTTGATTCGGTTCCAACGAAATTGGGTTTGATATTCAATTTGAGGCAGTGGGTTGGGAAATCAACGCCGAGTTGGGTTGAAGCGTATGTGAATGTGGAGTCTAATGGAGGTATGTCTTATAATTTTGGGACTTACGAATCAACTGTTTTGGATGTGACTAATGTTGATGCGTTGGTTCAATTGAGACAGTCAAGGACCCCTAGACGTGGCTCCAAGGTTAGCAAAATTATTTCTCTC

Coding sequence (CDS)

ATGGAGGCCGAGTATGACGAGAGGGCGGTTGAGAGTGGGAGTTTGGTGGTTTCTGGATGTGGGTTTGATTCGGTTCCAACGAAATTGGGTTTGATATTCAATTTGAGGCAGTGGGTTGGGAAATCAACGCCGAGTTGGGTTGAAGCGTATGTGAATGTGGAGTCTAATGGAGGTATGTCTTATAATTTTGGGACTTACGAATCAACTGTTTTGGATGTGACTAATGTTGATGCGTTGGTTCAATTGAGACAGTCAAGGACCCCTAGACGTGGCTCCAAGGTTAGCAAAATTATTTCTCTC

Protein sequence

MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVESNGGMSYNFGTYESTVLDVTNVDALVQLRQSRTPRRGSKVSKIISL
Homology
BLAST of CSPI03G38610 vs. ExPASy Swiss-Prot
Match: Q8LGI2 (Probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 OS=Arabidopsis thaliana OX=3702 GN=At5g39410 PE=1 SV=2)

HSP 1 Score: 110.5 bits (275), Expect = 1.1e-23
Identity = 52/90 (57.78%), Postives = 70/90 (77.78%), Query Frame = 0

Query: 1   MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVESNGGMS 60
           MEA Y +RA E+GSL+VS CGFDS+P +LGL+FN +QWV  S P+ +EAY+++ES+  ++
Sbjct: 133 MEANYHDRAEETGSLIVSACGFDSIPAELGLLFNAKQWVSPSVPNQIEAYLSLESDKKIA 192

Query: 61  YNFGTYESTVLDVTNVDALVQLRQSRTPRR 91
            NFGTYES VL V N + L +LR+SR PRR
Sbjct: 193 GNFGTYESAVLGVANAEKLKELRRSR-PRR 221

BLAST of CSPI03G38610 vs. ExPASy Swiss-Prot
Match: Q9GZE9 (Lipid droplet localized protein OS=Caenorhabditis elegans OX=6239 GN=ldp-1 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.7e-06
Identity = 31/93 (33.33%), Postives = 54/93 (58.06%), Query Frame = 0

Query: 1   MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVESN-GGM 60
           M+ +Y ++A E G  VVS CG+DS+P  LG+ F  + + G    + VE++V + +   G 
Sbjct: 123 MQQKYSKQAKEQGVYVVSACGWDSIPADLGVNFLKKNFHGDL--NHVESFVQLLTGPSGY 182

Query: 61  SYNFGTYESTVLDVTN--VDALVQLRQSRTPRR 91
           S+N GTY++ +L ++    D L  +R+   P +
Sbjct: 183 SFNAGTYQTLILGLSGAATDKLGAVRKEIMPEK 213

BLAST of CSPI03G38610 vs. ExPASy Swiss-Prot
Match: Q6AY30 (Saccharopine dehydrogenase-like oxidoreductase OS=Rattus norvegicus OX=10116 GN=Sccpdh PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.7e-05
Identity = 30/101 (29.70%), Postives = 52/101 (51.49%), Query Frame = 0

Query: 1   MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVESN-GGM 60
           M  +Y E+A E G  ++   GFDS+P  LG+++   Q  G  T   VE+++ + S   G+
Sbjct: 133 MHVKYHEKAAEKGVYIIGSSGFDSIPADLGVLYTRNQMNGTLTA--VESFLTINSGPEGL 192

Query: 61  SYNFGTYESTVLDVTNVDALVQLRQ----SRTPRRGSKVSK 97
             + GT++S +    +  +L +LR        P  GSK+ +
Sbjct: 193 CIHDGTWKSAIYGFGDKGSLRKLRSVSNLKPVPVIGSKLKR 231

BLAST of CSPI03G38610 vs. ExPASy Swiss-Prot
Match: Q8R127 (Saccharopine dehydrogenase-like oxidoreductase OS=Mus musculus OX=10090 GN=Sccpdh PE=1 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 2.3e-05
Identity = 26/84 (30.95%), Postives = 47/84 (55.95%), Query Frame = 0

Query: 1   MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVESN-GGM 60
           M A+Y E+A E G  ++   GFDS+P  LG+++   Q  G  T   VE+++ + +   G+
Sbjct: 133 MHAKYHEKAAEKGVYIIGSSGFDSIPADLGVLYTRNQMNGTLTA--VESFLTINTGPEGL 192

Query: 61  SYNFGTYESTVLDVTNVDALVQLR 84
             + GT++S +    +  +L +LR
Sbjct: 193 CIHDGTWKSAIYGFGDKGSLRKLR 214

BLAST of CSPI03G38610 vs. ExPASy Swiss-Prot
Match: Q5R5C9 (Saccharopine dehydrogenase-like oxidoreductase OS=Pongo abelii OX=9601 GN=SCCPDH PE=2 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 3.0e-05
Identity = 26/86 (30.23%), Postives = 48/86 (55.81%), Query Frame = 0

Query: 1   MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVESN-GGM 60
           M+ +Y E+A + G  ++   GFDS+P  LG+I+   +  G  T   VE+++ + S   G+
Sbjct: 133 MQLKYHEKAADKGVYIIGSSGFDSIPADLGVIYTRNKMNGTLTA--VESFLTIHSGPEGL 192

Query: 61  SYNFGTYESTVLDVTNVDALVQLRQS 86
           S + GT++S +    +   L +LR +
Sbjct: 193 SIHDGTWKSAIYGFGDQSNLRKLRNA 216

BLAST of CSPI03G38610 vs. ExPASy TrEMBL
Match: A0A0A0LG00 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G824480 PE=4 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 9.4e-47
Identity = 97/100 (97.00%), Postives = 98/100 (98.00%), Query Frame = 0

Query: 1   MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVESNGGMS 60
           MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVE NGGM+
Sbjct: 1   MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVECNGGMA 60

Query: 61  YNFGTYESTVLDVTNVDALVQLRQSRTPRRGSKVSKIISL 101
           YNFGTYESTVLDVTNVDALVQLRQSRTPRR SKVSKIISL
Sbjct: 61  YNFGTYESTVLDVTNVDALVQLRQSRTPRRRSKVSKIISL 100

BLAST of CSPI03G38610 vs. ExPASy TrEMBL
Match: A0A5D3DPV4 (Putative mitochondrial saccharopine dehydrogenase-like oxidoreductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007630 PE=4 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.8e-34
Identity = 74/93 (79.57%), Postives = 81/93 (87.10%), Query Frame = 0

Query: 1   MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVESNGGMS 60
           MEA+YD RAVE+GSLVVSGCGFDS+P +LGLIFNLR WVGKSTPSWVEAYVNVE + GM+
Sbjct: 136 MEAKYDGRAVETGSLVVSGCGFDSIPAELGLIFNLRHWVGKSTPSWVEAYVNVECSEGMT 195

Query: 61  YNFGTYESTVLDVTNVDALVQLRQSRTPRRGSK 94
           YNFGTYES VL V N DALVQLR+SR PRR  K
Sbjct: 196 YNFGTYESAVLGVANADALVQLRRSRPPRRRPK 228

BLAST of CSPI03G38610 vs. ExPASy TrEMBL
Match: A0A1S3B7Z4 (probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 OS=Cucumis melo OX=3656 GN=LOC103487170 PE=4 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.8e-34
Identity = 74/93 (79.57%), Postives = 81/93 (87.10%), Query Frame = 0

Query: 1   MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVESNGGMS 60
           MEA+YD RAVE+GSLVVSGCGFDS+P +LGLIFNLR WVGKSTPSWVEAYVNVE + GM+
Sbjct: 136 MEAKYDGRAVETGSLVVSGCGFDSIPAELGLIFNLRHWVGKSTPSWVEAYVNVECSEGMT 195

Query: 61  YNFGTYESTVLDVTNVDALVQLRQSRTPRRGSK 94
           YNFGTYES VL V N DALVQLR+SR PRR  K
Sbjct: 196 YNFGTYESAVLGVANADALVQLRRSRPPRRRPK 228

BLAST of CSPI03G38610 vs. ExPASy TrEMBL
Match: A0A6J1CTM9 (probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 OS=Momordica charantia OX=3673 GN=LOC111014111 PE=4 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 4.6e-25
Identity = 61/89 (68.54%), Postives = 71/89 (79.78%), Query Frame = 0

Query: 1   MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVESNGGMS 60
           MEAEY ERAVESGSLVVSGCGFDSVP +LG++FN RQWVGKS PS+VEAYV++ES   M+
Sbjct: 135 MEAEYHERAVESGSLVVSGCGFDSVPAELGVMFNSRQWVGKSAPSFVEAYVSLESRRRMA 194

Query: 61  YNFGTYESTVLDVTNVDALVQLRQSRTPR 90
            NFGT+ES VL V N   L +LR+S   R
Sbjct: 195 VNFGTFESAVLGVANATKLQKLRRSSPSR 223

BLAST of CSPI03G38610 vs. ExPASy TrEMBL
Match: A0A6J1I397 (probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 OS=Cucurbita maxima OX=3661 GN=LOC111468612 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 3.3e-23
Identity = 55/89 (61.80%), Postives = 70/89 (78.65%), Query Frame = 0

Query: 1   MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVESNGGMS 60
           MEA+Y ERAVE+GSLV+S CGFDSVP +LG +FN +QWVG+S P+ +EAY+++ES   + 
Sbjct: 127 MEADYHERAVENGSLVISACGFDSVPAELGFMFNSKQWVGESAPNRIEAYLSLESKNKIV 186

Query: 61  YNFGTYESTVLDVTNVDALVQLRQSRTPR 90
            NFGT+ES VL V N D LVQLR+SR  R
Sbjct: 187 GNFGTFESAVLGVANADQLVQLRRSRARR 215

BLAST of CSPI03G38610 vs. NCBI nr
Match: KAE8651205.1 (hypothetical protein Csa_002660 [Cucumis sativus])

HSP 1 Score: 186.4 bits (472), Expect = 1.2e-43
Identity = 90/97 (92.78%), Postives = 94/97 (96.91%), Query Frame = 0

Query: 1   MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVESNGGMS 60
           MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVE NGGM+
Sbjct: 67  MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVECNGGMA 126

Query: 61  YNFGTYESTVLDVTNVDALVQLRQSRTPRRGSKVSKI 98
           YNFGTYESTVLDVTNVDALVQLRQSRTPRR SK+ ++
Sbjct: 127 YNFGTYESTVLDVTNVDALVQLRQSRTPRRRSKLGQV 163

BLAST of CSPI03G38610 vs. NCBI nr
Match: XP_008443614.1 (PREDICTED: probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 [Cucumis melo] >KAA0053797.1 putative mitochondrial saccharopine dehydrogenase-like oxidoreductase [Cucumis melo var. makuwa] >TYK25604.1 putative mitochondrial saccharopine dehydrogenase-like oxidoreductase [Cucumis melo var. makuwa])

HSP 1 Score: 154.8 bits (390), Expect = 3.8e-34
Identity = 74/93 (79.57%), Postives = 81/93 (87.10%), Query Frame = 0

Query: 1   MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVESNGGMS 60
           MEA+YD RAVE+GSLVVSGCGFDS+P +LGLIFNLR WVGKSTPSWVEAYVNVE + GM+
Sbjct: 136 MEAKYDGRAVETGSLVVSGCGFDSIPAELGLIFNLRHWVGKSTPSWVEAYVNVECSEGMT 195

Query: 61  YNFGTYESTVLDVTNVDALVQLRQSRTPRRGSK 94
           YNFGTYES VL V N DALVQLR+SR PRR  K
Sbjct: 196 YNFGTYESAVLGVANADALVQLRRSRPPRRRPK 228

BLAST of CSPI03G38610 vs. NCBI nr
Match: XP_038905773.1 (probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 [Benincasa hispida])

HSP 1 Score: 142.5 bits (358), Expect = 2.0e-30
Identity = 69/90 (76.67%), Postives = 75/90 (83.33%), Query Frame = 0

Query: 1   MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVESNGGMS 60
           MEAEYD+RAVESGSLVVSGCGFDSVP +LGLI+N RQWVGKS PSWVEAY+NVES  GM 
Sbjct: 124 MEAEYDKRAVESGSLVVSGCGFDSVPAELGLIYNSRQWVGKSAPSWVEAYMNVESKKGMV 183

Query: 61  YNFGTYESTVLDVTNVDALVQLRQSRTPRR 91
            NFGT+ES VL V N D L Q R+SR PRR
Sbjct: 184 CNFGTFESAVLGVANADQLRQFRRSRPPRR 213

BLAST of CSPI03G38610 vs. NCBI nr
Match: XP_022144422.1 (probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 [Momordica charantia])

HSP 1 Score: 123.6 bits (309), Expect = 9.4e-25
Identity = 61/89 (68.54%), Postives = 71/89 (79.78%), Query Frame = 0

Query: 1   MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVESNGGMS 60
           MEAEY ERAVESGSLVVSGCGFDSVP +LG++FN RQWVGKS PS+VEAYV++ES   M+
Sbjct: 135 MEAEYHERAVESGSLVVSGCGFDSVPAELGVMFNSRQWVGKSAPSFVEAYVSLESRRRMA 194

Query: 61  YNFGTYESTVLDVTNVDALVQLRQSRTPR 90
            NFGT+ES VL V N   L +LR+S   R
Sbjct: 195 VNFGTFESAVLGVANATKLQKLRRSSPSR 223

BLAST of CSPI03G38610 vs. NCBI nr
Match: XP_022969659.1 (probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 [Cucurbita maxima])

HSP 1 Score: 117.5 bits (293), Expect = 6.7e-23
Identity = 55/89 (61.80%), Postives = 70/89 (78.65%), Query Frame = 0

Query: 1   MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVESNGGMS 60
           MEA+Y ERAVE+GSLV+S CGFDSVP +LG +FN +QWVG+S P+ +EAY+++ES   + 
Sbjct: 127 MEADYHERAVENGSLVISACGFDSVPAELGFMFNSKQWVGESAPNRIEAYLSLESKNKIV 186

Query: 61  YNFGTYESTVLDVTNVDALVQLRQSRTPR 90
            NFGT+ES VL V N D LVQLR+SR  R
Sbjct: 187 GNFGTFESAVLGVANADQLVQLRRSRARR 215

BLAST of CSPI03G38610 vs. TAIR 10
Match: AT5G39410.1 (Saccharopine dehydrogenase )

HSP 1 Score: 110.5 bits (275), Expect = 7.7e-25
Identity = 52/90 (57.78%), Postives = 70/90 (77.78%), Query Frame = 0

Query: 1   MEAEYDERAVESGSLVVSGCGFDSVPTKLGLIFNLRQWVGKSTPSWVEAYVNVESNGGMS 60
           MEA Y +RA E+GSL+VS CGFDS+P +LGL+FN +QWV  S P+ +EAY+++ES+  ++
Sbjct: 133 MEANYHDRAEETGSLIVSACGFDSIPAELGLLFNAKQWVSPSVPNQIEAYLSLESDKKIA 192

Query: 61  YNFGTYESTVLDVTNVDALVQLRQSRTPRR 91
            NFGTYES VL V N + L +LR+SR PRR
Sbjct: 193 GNFGTYESAVLGVANAEKLKELRRSR-PRR 221

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LGI21.1e-2357.78Probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 ... [more]
Q9GZE92.7e-0633.33Lipid droplet localized protein OS=Caenorhabditis elegans OX=6239 GN=ldp-1 PE=1 ... [more]
Q6AY301.7e-0529.70Saccharopine dehydrogenase-like oxidoreductase OS=Rattus norvegicus OX=10116 GN=... [more]
Q8R1272.3e-0530.95Saccharopine dehydrogenase-like oxidoreductase OS=Mus musculus OX=10090 GN=Sccpd... [more]
Q5R5C93.0e-0530.23Saccharopine dehydrogenase-like oxidoreductase OS=Pongo abelii OX=9601 GN=SCCPDH... [more]
Match NameE-valueIdentityDescription
A0A0A0LG009.4e-4797.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G824480 PE=4 SV=1[more]
A0A5D3DPV41.8e-3479.57Putative mitochondrial saccharopine dehydrogenase-like oxidoreductase OS=Cucumis... [more]
A0A1S3B7Z41.8e-3479.57probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 ... [more]
A0A6J1CTM94.6e-2568.54probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 ... [more]
A0A6J1I3973.3e-2361.80probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 ... [more]
Match NameE-valueIdentityDescription
KAE8651205.11.2e-4392.78hypothetical protein Csa_002660 [Cucumis sativus][more]
XP_008443614.13.8e-3479.57PREDICTED: probable mitochondrial saccharopine dehydrogenase-like oxidoreductase... [more]
XP_038905773.12.0e-3076.67probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 ... [more]
XP_022144422.19.4e-2568.54probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 ... [more]
XP_022969659.16.7e-2361.80probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 ... [more]
Match NameE-valueIdentityDescription
AT5G39410.17.7e-2557.78Saccharopine dehydrogenase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR12286:SF9MITOCHONDRIAL SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE-RELATEDcoord: 1..90
NoneNo IPR availablePANTHERPTHR12286UNCHARACTERIZEDcoord: 1..90

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G38610.1CSPI03G38610.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009247 glycolipid biosynthetic process
cellular_component GO:0005811 lipid droplet
cellular_component GO:0016020 membrane
molecular_function GO:0016491 oxidoreductase activity