CSPI03G37350 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G37350
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Cytoplasmic tRNA 2-thiolation protein 2
Location	Chr3: 32380478 .. 32384296 (-)
RNA-Seq Expression	CSPI03G37350
Synteny	CSPI03G37350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTAAAATGTAACCATTTTTTAGGTTTTAGATAAACCCTGGTGGGGAGCGTTTACACAATTTGAAAGCTTGGAACGAAGAAGATTTGTCGCTCTGAATCTTGAGGCGTTTTAATCTCTACCATTCATGGCATGTAATTCCTCAGCCTGCCAGTCCAACTGCTACAGAAATCAACATGATGAACATACAATCGAACAAGATTCACCTCCTTCAAGCAGGCCCGATACACCAGCTGCCATGGAGGCTCGTCGAGTTATCACGAAAGACGACAATAGTAATACGAATCTCTGCTTTAAATGTAAGTCCAAGGAACGTATGTCTACCTCCGGAGTAGGAGACGATGGACGATTTTGCAGTGATTGCTTTCGGAGCAACTTGTTTGCTAAGTTCCGGCAGGCTGTAACCTCTAACGCCATGATCTCTCCTTCTGATAAAGTGCTTGTTGCGTTCTCTGGAGGCCCTTGTTCCAGGTATTTTCTCTATTTATATATATTGTGTATGTATGTATGGATGGATGGATATTTGGACGTTGCTAGTGGGCATAATAGATTTTACGGCTGAATTTTTTGTTAGAATCTATAGTGCCAATGAGTTTGTAATGTTTTGATGTTATTCGTGATCGACTTAACCTCCCTTCCCAATCACTTCCAGGGTGGCTTTGCAGTTTGTTCATGAAATGCAAGTAAAAGCACACAATAATTTTGAAGCAAGTAGGGATAGATCACTGCAAGTTTTTGGTGTCGGAGTTGCTTATATCAATGAAACTGCTGCTTGTTCTATCCCTTCGGACGAGATAGACAAAACAGTTCAAACCATCCAATCGGTTGTGTCAAGTCTCTGTCAACCAAGAAAAAATTTGCATATTGTTCAAATTGAGAGTGTATACTCTTCAAATTTGAATGATGGAAGGGAGAAGTTGAAGAAGCTGTTAGATGCAGTCAATGACGTCACAGGAAAAGAAGACCTTATCTCATATTTGCGGATGTTATCCTTGCAAAAAGTTTGCCCCTCAATTGTTCCTTCATTTAGCTTGCAGTGAAAGAGGGGGAAATTCTCAGCCTGTTGTTTGTTGTGCAGATTGCAGCTGAAAATGGGTACAACAGACTTCTAGTTGGAACATGTACTTCAAGGATTGCATGCCATGTGATTTCGTCTACGGTTAAGGTCTTCCTTCGTTTGTTACCCCCCCCCCCCCTCTAATATTGATTTGTATTTTAAGGGTCTATTTTGGAAGTTGAGTCACTAGAATTTTTAGAGTCATGATAATGAATAACCAGAAGGAAGGTTAGAAGGTTGATGCATTGACTGTTAAGGGTACATGGATACGAGAACTCTGTTATTTGAGATAGAACAGAACAATTGGTCTATGCTTCAATCTTAGATCTTGAGGATATTATGTAAATAAGATCCAGTAATATGGCAAGCAAACACTTTGCTTATACAAAAATCTGTTTCCATGCAAAAAAAATCCTGTGAAAATTAAACCCTCAAACTCACGTAGTTGTAGAAATTATAACAAATGCTCAAGTTTGAGGGTTTGACTTTTAGCATTCGAGGGTCCAATTTCTGTAACCAGTGTAAGTTTGTGGGCGGCTTAATATTTACCATTGAAAACTCGAGGATATAATTCCAGTTACCACCATGGTTCAAAAGTGATTCTTACAATTTGTCCACAATAATATCATTTTTATTGGATTATTAAGTAAGAGCTTGCCATGCACACTTTTATTTATCGTTCAGACAAAGTCAAGAGTGGAACTACTCTCTGCAGGGAAAACTGGGATGCTTGAAGGGGAGAAAGAGGGGCATCATTATCTTTCAGTAGACACCTCTATTAAGAGCTAAAAAATAGCTAGGGTAGCTATGGAATCTTATGATGAATTATCTTGTTGTTTGAGATTTCACTGCTACTTATTACAAGCTGCTTTTCTTTTTCTACTTGAAAATCTTTCCGTTTGAGTTAGGTCCATGCACTAATTTTGACTACACGAGTTTAATTCAAGCAGGGCCAAGGGTATTCCTTGCCGGGAGATGTACAGTATATTGATGCAAGGTGGGAGGTTCCAGTTGTACTTCCTCTACTTGACTGTCTTGCGCAAGAGCTCAACATGCTTTGCTGCGTTGATGGGTCTGTTAACTTTGTCATGTTTAGTACTTTACTGTTGTTAAATTTACTTGCTGTTGTTGCATCTTTAATATGTTCACTTCGACTGAATCTAATTGATGATGGGAGCCGTTTCTTGGCAGGGCAGTCTAAAGACAGTGGAACTGACCAAAAAGTCCAGTTCTGGCATCAATGACCTGGTCTCCTCATTTGTGGCTCTTTTGCAGGTACACGTAGGAATTTGATTTAGAAAAATGTGCAGTTTTATTCATTTTGGCTGGAGAGCAACTTCTTGAAGCATGTTTTGTGTTCAATTTAGGAGCAATTTTGGTAGAGTATGGAAACAATTTTACCTTCTATTTTGAGTGATTGTTTCCAAGTAAGAAAGAAATTGTGCATACAAGTTGTAGGCTACTTGTCCTACCCAGGACTGTTTTTCAGAACTTGACCGAAGATTTTTTTTCTTTTTCTACTTGCCTTTATAGGTAGAAAATCCTTCTCGTGAGTCTACAATTGTGAGAACTGCTGGAAAGCTTACTCCATTCAATTTTAGCAGAATACCAGATTTGAACGAAAGTAATAATGTTCCTTTGGCCACGAAAAGGCGCCAACAGAGAAATAACTTTAAGCATGTTAACTCTTTATCTTCGGAGTCATTTTGTGCAGTCTGTAATGGGCCACTTAACACGTCAGATGTGCTAACTCGCCATGCAGGTTCAAGTATTTTAGGTGCTTGTTGTTCAAGTTGTCAATTTCAGATTCTTCCCAAGGATTCTTCATCTCAAGAACTGTTTCTTTCACTCTTGCCACCCTCTATGGTTGCTCGAGCCACACATGGCAGCCTTGGCAATTCCAATTCGCTAAGGTGAGAGTGAGATTAAGTCTTGTATTACTGTCGGTACTACATAGTTCAATTTTATGATAACTGAATATTGTAATCTAAATCTTTGTTGTATATCATCTTTCTAAAAGAGGTTTCCCTTCAATTCTGGACCGAAGCACATCCAAAGTAAAATATAAGGAAAATTAAACGACTTCAGCCTGTGGTTTGCATGATTCAAAAAATGTCTCTGAGTCGTGTTAGGTGGACTTGATGAAGTTATTGAAATAATGTTGCTTTATTCTGTAAATAGTAATGTGCTTAAAGTGTATACTAGCATGAAGTCTGCTTCAGATATTACTATTTTGAACTTATTAAGTAATGCTTTGAAAATATCCCCGCTAAATAGTTATGTTATTAATTGCAGGAAGAAAATACAGGAGTTCTTACTCTCAGATGATGAAGCTGAATCATAATTCAGGAGTTATTTTCCCTCAGTTCAACAACTGGTAATATTGCCTTGTGATCTATAAATTGAATTGATTGCTAATTAGGGAATCAAGGGTGAATTCAAGATATAATTGACTTCTGTGCATCCTCATTGTTCTTTCTATGGCTATTGGTTTCCATAATGAAGGATCAGAAGTCCACATTGAGAAGGAACCAAGGGCGAAACCAACTATTAGTCACTAGAGTTGTCTCTAATAAAACCATGTCTGGAGACCGCACATTATATAAAATTTATTTTCCTATTTTTTAAATATATTGGGTTGTTGAAACTTGAAACTCTAATGTTAGTTAGAGATATAGGAAAAGAGTTTATTGTGGTCTGCCTTGTCTAGTCATTTATTGAAAGCTCTCAAACCTATTATGCATAAAAAGAAAAGAAACCTTACC

mRNA sequence

GTAAAATGTAACCATTTTTTAGGTTTTAGATAAACCCTGGTGGGGAGCGTTTACACAATTTGAAAGCTTGGAACGAAGAAGATTTGTCGCTCTGAATCTTGAGGCGTTTTAATCTCTACCATTCATGGCATGTAATTCCTCAGCCTGCCAGTCCAACTGCTACAGAAATCAACATGATGAACATACAATCGAACAAGATTCACCTCCTTCAAGCAGGCCCGATACACCAGCTGCCATGGAGGCTCGTCGAGTTATCACGAAAGACGACAATAGTAATACGAATCTCTGCTTTAAATGTAAGTCCAAGGAACGTATGTCTACCTCCGGAGTAGGAGACGATGGACGATTTTGCAGTGATTGCTTTCGGAGCAACTTGTTTGCTAAGTTCCGGCAGGCTGTAACCTCTAACGCCATGATCTCTCCTTCTGATAAAGTGCTTGTTGCGTTCTCTGGAGGCCCTTGTTCCAGGGTGGCTTTGCAGTTTGTTCATGAAATGCAAGTAAAAGCACACAATAATTTTGAAGCAAGTAGGGATAGATCACTGCAAGTTTTTGGTGTCGGAGTTGCTTATATCAATGAAACTGCTGCTTGTTCTATCCCTTCGGACGAGATAGACAAAACAGTTCAAACCATCCAATCGGTTGTGTCAAGTCTCTGTCAACCAAGAAAAAATTTGCATATTGTTCAAATTGAGAGTGTATACTCTTCAAATTTGAATGATGGAAGGGAGAAGTTGAAGAAGCTGTTAGATGCAGTCAATGACGTCACAGGAAAAGAAGACCTTATCTCATATTTGCGGATGTTATCCTTGCAAAAAATTGCAGCTGAAAATGGGTACAACAGACTTCTAGTTGGAACATGTACTTCAAGGATTGCATGCCATGTGATTTCGTCTACGGTTAAGGGCCAAGGGTATTCCTTGCCGGGAGATGTACAGTATATTGATGCAAGGTGGGAGGTTCCAGTTGTACTTCCTCTACTTGACTGTCTTGCGCAAGAGCTCAACATGCTTTGCTGCGTTGATGGTCTAAAGACAGTGGAACTGACCAAAAAGTCCAGTTCTGGCATCAATGACCTGGTCTCCTCATTTGTGGCTCTTTTGCAGGTAGAAAATCCTTCTCGTGAGTCTACAATTGTGAGAACTGCTGGAAAGCTTACTCCATTCAATTTTAGCAGAATACCAGATTTGAACGAAAGTAATAATGTTCCTTTGGCCACGAAAAGGCGCCAACAGAGAAATAACTTTAAGCATGTTAACTCTTTATCTTCGGAGTCATTTTGTGCAGTCTGTAATGGGCCACTTAACACGTCAGATGTGCTAACTCGCCATGCAGGTTCAAGTATTTTAGGTGCTTGTTGTTCAAGTTGTCAATTTCAGATTCTTCCCAAGGATTCTTCATCTCAAGAACTGTTTCTTTCACTCTTGCCACCCTCTATGGTTGCTCGAGCCACACATGGCAGCCTTGGCAATTCCAATTCGCTAAGGAAGAAAATACAGGAGTTCTTACTCTCAGATGATGAAGCTGAATCATAATTCAGGAGTTATTTTCCCTCAGTTCAACAACTGGTAATATTGCCTTGTGATCTATAAATTGAATTGATTGCTAATTAGGGAATCAAGGGTGAATTCAAGATATAATTGACTTCTGTGCATCCTCATTGTTCTTTCTATGGCTATTGGTTTCCATAATGAAGGATCAGAAGTCCACATTGAGAAGGAACCAAGGGCGAAACCAACTATTAGTCACTAGAGTTGTCTCTAATAAAACCATGTCTGGAGACCGCACATTATATAAAATTTATTTTCCTATTTTTTAAATATATTGGGTTGTTGAAACTTGAAACTCTAATGTTAGTTAGAGATATAGGAAAAGAGTTTATTGTGGTCTGCCTTGTCTAGTCATTTATTGAAAGCTCTCAAACCTATTATGCATAAAAAGAAAAGAAACCTTACC

Coding sequence (CDS)

ATGGCATGTAATTCCTCAGCCTGCCAGTCCAACTGCTACAGAAATCAACATGATGAACATACAATCGAACAAGATTCACCTCCTTCAAGCAGGCCCGATACACCAGCTGCCATGGAGGCTCGTCGAGTTATCACGAAAGACGACAATAGTAATACGAATCTCTGCTTTAAATGTAAGTCCAAGGAACGTATGTCTACCTCCGGAGTAGGAGACGATGGACGATTTTGCAGTGATTGCTTTCGGAGCAACTTGTTTGCTAAGTTCCGGCAGGCTGTAACCTCTAACGCCATGATCTCTCCTTCTGATAAAGTGCTTGTTGCGTTCTCTGGAGGCCCTTGTTCCAGGGTGGCTTTGCAGTTTGTTCATGAAATGCAAGTAAAAGCACACAATAATTTTGAAGCAAGTAGGGATAGATCACTGCAAGTTTTTGGTGTCGGAGTTGCTTATATCAATGAAACTGCTGCTTGTTCTATCCCTTCGGACGAGATAGACAAAACAGTTCAAACCATCCAATCGGTTGTGTCAAGTCTCTGTCAACCAAGAAAAAATTTGCATATTGTTCAAATTGAGAGTGTATACTCTTCAAATTTGAATGATGGAAGGGAGAAGTTGAAGAAGCTGTTAGATGCAGTCAATGACGTCACAGGAAAAGAAGACCTTATCTCATATTTGCGGATGTTATCCTTGCAAAAAATTGCAGCTGAAAATGGGTACAACAGACTTCTAGTTGGAACATGTACTTCAAGGATTGCATGCCATGTGATTTCGTCTACGGTTAAGGGCCAAGGGTATTCCTTGCCGGGAGATGTACAGTATATTGATGCAAGGTGGGAGGTTCCAGTTGTACTTCCTCTACTTGACTGTCTTGCGCAAGAGCTCAACATGCTTTGCTGCGTTGATGGTCTAAAGACAGTGGAACTGACCAAAAAGTCCAGTTCTGGCATCAATGACCTGGTCTCCTCATTTGTGGCTCTTTTGCAGGTAGAAAATCCTTCTCGTGAGTCTACAATTGTGAGAACTGCTGGAAAGCTTACTCCATTCAATTTTAGCAGAATACCAGATTTGAACGAAAGTAATAATGTTCCTTTGGCCACGAAAAGGCGCCAACAGAGAAATAACTTTAAGCATGTTAACTCTTTATCTTCGGAGTCATTTTGTGCAGTCTGTAATGGGCCACTTAACACGTCAGATGTGCTAACTCGCCATGCAGGTTCAAGTATTTTAGGTGCTTGTTGTTCAAGTTGTCAATTTCAGATTCTTCCCAAGGATTCTTCATCTCAAGAACTGTTTCTTTCACTCTTGCCACCCTCTATGGTTGCTCGAGCCACACATGGCAGCCTTGGCAATTCCAATTCGCTAAGGAAGAAAATACAGGAGTTCTTACTCTCAGATGATGAAGCTGAATCATAA

Protein sequence

MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKSKERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQPRKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNRLLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVDGLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNNVPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHAGSSILGACCSSCQFQILPKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES*

Homology

[Close]

BLAST of CSPI03G37350 vs. ExPASy Swiss-Prot
Match: O65628 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Arabidopsis thaliana OX=3702 GN=CTU2 PE=2 SV=3)

HSP 1 Score: 493.4 bits (1269), Expect = 2.8e-138
Identity = 260/473 (54.97%), Postives = 340/473 (71.88%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60
           MACNSS C+S CY  + D  +   D   S                    ++ ++C KCK 
Sbjct: 1   MACNSSGCESGCYDREKDNGSKIVDDAVSG-----------------GGNHESVCVKCKC 60

Query: 61  KERMSTSGVG-DDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQ 120
              M+    G DDGRFC+DCFR+N+F KFR AVTS+AMI+PSD VLVAFSGG  SRV+LQ
Sbjct: 61  NAPMTFGDGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQ 120

Query: 121 FVHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQ 180
           FVHE+Q+KA  N+EASRDRSL VFGVGVA+++ETAA    S E+   ++ ++  VS L  
Sbjct: 121 FVHELQIKALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSP 180

Query: 181 PRKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYN 240
           P K+LH+V +ES++ S+  D R++L KLLD+V D TGKEDL+ +L+MLSLQK+AAENGYN
Sbjct: 181 PAKDLHVVPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKVAAENGYN 240

Query: 241 RLLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCV 300
           RL++G+CTSRIA HV+++TVKG+GYSL  D+Q++DARW+VP+VLPL DC+  E+  LC +
Sbjct: 241 RLVLGSCTSRIASHVLTATVKGRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCLL 300

Query: 301 DGLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESN 360
           DGLKTVEL  +S  GINDLVSSFVALLQ ENPSRE TIVRTA KLTPF F++IP+ ++S 
Sbjct: 301 DGLKTVELACRSQCGINDLVSSFVALLQEENPSRECTIVRTAAKLTPFYFNKIPETDDS- 360

Query: 361 NVPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSD---VLTRHAGSS---ILGACCS 420
           NVP+AT+RR +R N K+  S+++E+FC +CNGPLN SD   + T   G     +  ACCS
Sbjct: 361 NVPMATQRRLKRFNLKYDGSMTTEAFCPICNGPLNRSDSSELDTFEEGQESDVLYAACCS 420

Query: 421 SCQFQILPKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDE 467
           SC+FQILP+D SS E F S LP  M+++  H  + +   LR+KI++ LL DDE
Sbjct: 421 SCRFQILPQDGSSLEQFSSFLPDHMISQVKHQKVDSQAYLREKIKDCLLLDDE 455

[Close]

BLAST of CSPI03G37350 vs. ExPASy Swiss-Prot
Match: Q2QMW0 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CTU2 PE=2 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 6.5e-103
Identity = 195/400 (48.75%), Postives = 272/400 (68.00%), Query Frame = 0


Query: 76  CSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVKAHNNFEAS 135
           C +CFR+NL  KF+ AVTSNAM+ P+D VL+AFSGGP SRVALQF+HEM+ KA  +++ S
Sbjct: 66  CGECFRANLLGKFKLAVTSNAMVRPTDSVLLAFSGGPASRVALQFIHEMRCKAIESWDVS 125

Query: 136 RDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQPRKNLHIVQIESVYSS 195
             ++L VFGVGVA+++E+  CS P DEI+  ++ I+S+VSSL    K +HI ++E V+S+
Sbjct: 126 NSQALPVFGVGVAFVDESVLCSKPRDEIEMAIEDIRSIVSSLSTGVKAMHIARLEDVFST 185

Query: 196 NLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNRLLVGTCTSRIACHVI 255
              DG  +L++ +D + D TG+ED +  LRMLSLQKIA ENGY ++++G+C S IACHV+
Sbjct: 186 ESEDGERRLREAVDMIGDDTGREDFLRCLRMLSLQKIAMENGYAKIMLGSCASAIACHVL 245

Query: 256 SSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVDGLKTVELTKKSSSGI 315
           S+TVKGQGYSLP DVQY+D RWE+PVVLPL DCLAQEL +LC +D LKT +   + S+GI
Sbjct: 246 SATVKGQGYSLPADVQYVDTRWEIPVVLPLRDCLAQELTLLCELDSLKTQQHLDRPSNGI 305

Query: 316 NDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNNVPLATKRRQQRNNFK 375
           N LV+SF+  L+ ENPSRE TIVRTA KL PF+F++       + +P  ++ R +   F 
Sbjct: 306 NSLVASFIKRLREENPSREHTIVRTAQKLKPFSFNKFSADGYHDFLP--SRLRPKFQKFD 365

Query: 376 HVNSLSSESFCAVCNGPLNTSDV----LTRHAGSSIL----GACCSSCQFQILPKDSSSQ 435
              S  SE  C +C  P + S++     T+H     +      CC SC FQILP   +  
Sbjct: 366 SDESTFSEILCLMCGSPFSESELQNLESTKHKAQKKIDLYTAHCCQSCYFQILPAGENLN 425

Query: 436 ELFLSLLPPSMVARATHGSLGNSNS-LRKKIQEFLLSDDE 467
           E F SLLP     +    ++ +S+S LR +I+E+LL +++
Sbjct: 426 EHFFSLLPKLWTGKM--DTISDSHSLLRDQIEEYLLEEND 461

[Close]

BLAST of CSPI03G37350 vs. ExPASy Swiss-Prot
Match: A2ZM73 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=CTU2 PE=3 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.4e-102
Identity = 195/400 (48.75%), Postives = 272/400 (68.00%), Query Frame = 0


Query: 76  CSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVKAHNNFEAS 135
           C +CFR+NL  KF+ AVTSNAM+ P+D VL+AFSGGP SRVALQF+HEM+ KA  +++ S
Sbjct: 66  CGECFRANLLGKFKLAVTSNAMVRPTDSVLLAFSGGPASRVALQFIHEMRCKAIESWDVS 125

Query: 136 RDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQPRKNLHIVQIESVYSS 195
             ++L VFGVGVA+++E+  CS P DEI+  ++ I+S+VSSL    K +HIV++E V+S+
Sbjct: 126 NSQALPVFGVGVAFVDESVLCSKPRDEIEMAIEDIRSIVSSLSTGVKAMHIVRLEDVFST 185

Query: 196 NLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNRLLVGTCTSRIACHVI 255
              DG  +L++ +D + D TG+ED +  LRMLSLQKIA ENGY ++++G+C S IACHV+
Sbjct: 186 ESEDGERRLREAVDMIGDDTGREDFLRCLRMLSLQKIAMENGYAKIMLGSCASAIACHVL 245

Query: 256 SSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVDGLKTVELTKKSSSGI 315
           S+TVKGQGYSLP DVQY+D RWE+PVVLPL DCLAQEL +LC +D LKT +   + S+GI
Sbjct: 246 SATVKGQGYSLPADVQYVDTRWEIPVVLPLRDCLAQELTLLCELDSLKTQQHLDRPSNGI 305

Query: 316 NDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNNVPLATKRRQQRNNFK 375
           N LV+SF+  L+ ENPSRE TIVRTA KL PF+F++       + +P  ++ R +     
Sbjct: 306 NSLVASFIKRLREENPSREHTIVRTAQKLKPFSFNKFSADGYHDFLP--SRLRPKFQKVD 365

Query: 376 HVNSLSSESFCAVCNGPLNTSDV----LTRHAGSSIL----GACCSSCQFQILPKDSSSQ 435
              S  SE  C +C  P + S++     T+H     +      CC SC FQILP   +  
Sbjct: 366 SDESTFSEILCLMCGSPFSESELQNLESTKHKAQKKIDLYTAHCCQSCYFQILPAGENLN 425

Query: 436 ELFLSLLPPSMVARATHGSLGNSNS-LRKKIQEFLLSDDE 467
           E F SLLP     +    ++ +S+S LR +I+E+LL +++
Sbjct: 426 EHFFSLLPKLWTGKM--DTISDSHSLLRDQIEEYLLEEND 461

[Close]

BLAST of CSPI03G37350 vs. ExPASy Swiss-Prot
Match: Q08B12 (Cytoplasmic tRNA 2-thiolation protein 2-A OS=Xenopus laevis OX=8355 GN=ctu2-a PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.7e-23
Identity = 135/522 (25.86%), Postives = 209/522 (40.04%), Query Frame = 0


Query: 46  KDDNSNTNLCFKCKSKERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVL 105
           K   S   +C KCK          GD   FC  CF+     KFR  +  N +I P +KVL
Sbjct: 16  KQGKSLGKICMKCKESSAALLIRAGD--AFCKSCFKEYFVHKFRATLGKNRVIYPGEKVL 75

Query: 106 VAFSGGPCSRVALQFVHEMQVKAHNNFEASRDRSLQV-FGVGVAYINETAACSIPSDEID 165
           +A+SGGP S   ++ V E           SRD   ++ F  G+ +I+E  AC +  +E  
Sbjct: 76  LAYSGGPSSSAMVRQVQE---------GLSRDAPKKLRFVPGILFIDEGTACGMSWEE-- 135

Query: 166 KTVQTIQSVVSSLCQPRKNLHIVQIESVYS---SNLNDG--------------------- 225
              Q +  + S L Q +   HIV +E V+S   S L  G                     
Sbjct: 136 -RQQILSEICSVLQQTKIPFHIVSLEQVFSLPGSVLQRGAPEQRPNYKEEVDRFLVQERE 195

Query: 226 ---------------------------------------REKLKKLLDAVNDVTGKEDLI 285
                                                   +KLK+L  +   +T K+ L+
Sbjct: 196 QGDAGCSEMLERLEVTDSDSPGSSDKMYQSTCSHPPDMHTQKLKQLFASAKTLTAKQQLL 255

Query: 286 SYLRMLSLQKIAAENGYNRLLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARW-EVP 345
             LR   +  IA   GY++++ G   +R++  ++S+   G+G  LP D  + D+R+ +V 
Sbjct: 256 HTLRSHLILHIARTCGYSKVMTGESCTRLSIRLLSNVSLGRGAFLPLDTGFCDSRYGDVD 315

Query: 346 VVLPLLDCLAQEL---NMLCCVDGLKTVELTKKSS--SGINDLVSSFVALLQVENPSRES 405
           ++ P+ +  ++E+   N    V  +    L  K+S  S I  L   FV  LQ + PS  S
Sbjct: 316 IIRPMREYSSKEIAYYNRFFNVSPIFIPALDTKASENSSIQHLTEVFVNRLQADFPSTVS 375

Query: 406 TIVRTAGKLTPFNFSRIPDLNESNN--------VPLATKRRQ----QRNNFKH------- 465
           T+ RT+ KL   N S+I    E+           PL T+  +          H       
Sbjct: 376 TLYRTSEKL---NVSKIDADQETCAKDRCLLCLSPLDTQAGKASAFSATQLSHHLSQKIP 435

Query: 466 --VNSLSSESFCAVCNG-----PLNTSDVLTRHA--GSSILGACCSSCQFQILPKDSSSQ 470
              N L++ S  + C G          D     A    S +   C SC+  +  KD  S 
Sbjct: 436 MKSNDLANNSDKSCCQGGQGCKEAGYGDTCQSRALQTPSFVHMLCYSCRLTV--KDMQS- 495

[Close]

BLAST of CSPI03G37350 vs. ExPASy Swiss-Prot
Match: Q32NV1 (Cytoplasmic tRNA 2-thiolation protein 2-B OS=Xenopus laevis OX=8355 GN=ctu2-b PE=2 SV=2)

HSP 1 Score: 105.9 bits (263), Expect = 1.3e-21
Identity = 134/522 (25.67%), Postives = 208/522 (39.85%), Query Frame = 0


Query: 46  KDDNSNTNLCFKCKSKERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVL 105
           K   S   +C KCK          GD   FC  CF+     KFR  +  N +I P +KVL
Sbjct: 16  KQAKSLGKICMKCKESSAALLIRAGD--AFCKSCFKEYFVHKFRATLGKNRVIYPGEKVL 75

Query: 106 VAFSGGPCSRVALQFVHEMQVKAHNNFEASRDRSLQV-FGVGVAYINETAACSIPSDEID 165
           +A+SGGP S   ++ V E           SRD   ++ F  G+ +I+E  AC +  +E  
Sbjct: 76  LAYSGGPSSSAMVRQVQE---------GLSRDAPKKLRFVPGILFIDEGTACGMSWEE-- 135

Query: 166 KTVQTIQSVVSSLCQPRKNLHIVQIESVYS---SNLNDG--------------------- 225
              Q +  + S L Q +   HIV +E V+S   S L  G                     
Sbjct: 136 -RQQILSEICSVLQQTKIPFHIVSLEQVFSLPGSVLQRGAPEQRPNYKEEVDRFLVQERE 195

Query: 226 ---------------------------------------REKLKKLLDAVNDVTGKEDLI 285
                                                   +KLK+L  +   +T K+ L+
Sbjct: 196 QGDAGCSEMLERLEVTDSDSPGSSDKMYQSTCSRPPDMHTQKLKQLFASAKTLTAKQQLL 255

Query: 286 SYLRMLSLQKIAAENGYNRLLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARW-EVP 345
             LR   +  IA   GY++++ G   +R++  ++S+   G+G  LP D  + D+R+ +V 
Sbjct: 256 HTLRSHLILHIARTCGYSKVMTGESCTRLSIRLLSNVSLGRGAFLPLDTGFCDSRYGDVD 315

Query: 346 VVLPLLDCLAQEL---NMLCCVDGLKTVELTKKSS--SGINDLVSSFVALLQVENPSRES 405
           ++ P+ +  ++E+   N    V  +    L  K+S  S I  L   FV  LQ + PS  S
Sbjct: 316 IIRPMREYSSKEIAYYNRFFNVLPIFIPALDTKASENSSIQHLTEVFVNRLQADFPSTVS 375

Query: 406 TIVRTAGKLTPFNFSRIPDLNESNN--------VPLATKRRQ----QRNNFKH------- 465
           T+ RT+ KL   N S I    E+           PL T+  +          H       
Sbjct: 376 TLYRTSEKL---NVSIIDADQETCAKDRCLLCLSPLDTQAGKASAFSATQLSHHLSQKIP 435

Query: 466 --VNSLSSESFCAVCNG-----PLNTSDVLTRHA--GSSILGACCSSCQFQILPKDSSSQ 470
              N L++ S  + C G          D     A    S +   C SC+  +  KD  S 
Sbjct: 436 MKSNDLANNSDKSCCQGGQGCKEAGYGDTCQSRALQTPSFVHMLCYSCRLTV--KDMQS- 495

[Close]

BLAST of CSPI03G37350 vs. ExPASy TrEMBL
Match: A0A0A0LC57 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis sativus OX=3659 GN=Csa_3G816060 PE=3 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 3.6e-266
Identity = 469/469 (100.00%), Postives = 469/469 (100.00%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60

Query: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180
           VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP
Sbjct: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180

Query: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD 300
           LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD
Sbjct: 241 LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN
Sbjct: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHAGSSILGACCSSCQFQIL 420
           VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHAGSSILGACCSSCQFQIL
Sbjct: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHAGSSILGACCSSCQFQIL 420

Query: 421 PKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES 470
           PKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES
Sbjct: 421 PKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES 469

[Close]

BLAST of CSPI03G37350 vs. ExPASy TrEMBL
Match: A0A1S3B7H9 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo OX=3656 GN=LOC103486848 PE=3 SV=1)

HSP 1 Score: 864.8 bits (2233), Expect = 1.7e-247
Identity = 441/469 (94.03%), Postives = 451/469 (96.16%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDEHTIEQDSPPSSRP T A  E+R  IT DDNSNTNLCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGESR--ITNDDNSNTNLCFKCKS 60

Query: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMSTSGVGDDGR CSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180
           VHE+QVKAH NFEASRDRSLQVFGVGVAYINETAA SIPSDEIDKT+QTIQSVVSSL QP
Sbjct: 121 VHEIQVKAHKNFEASRDRSLQVFGVGVAYINETAAYSIPSDEIDKTIQTIQSVVSSLSQP 180

Query: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RK+LHIV IE+VYSSNL+DGREKLKKLLDAV+DVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKDLHIVPIENVYSSNLSDGREKLKKLLDAVDDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD 300
           LLVGTCTSRIACHVISSTVKGQGYSLP DVQYIDARWEVPVVLPLLDCLAQELNMLCC+D
Sbjct: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCCID 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN
Sbjct: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHAGSSILGACCSSCQFQIL 420
           VPLATKRRQ+RNNFKHVNSLSSESFCAVCNGPLNTSDV TRHA SSILGACCSSCQFQIL
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHASSSILGACCSSCQFQIL 420

Query: 421 PKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES 470
           PKDS S ELFLSLLPPSMVARATHGSLGNSNSLR KIQEFLLSDD+AES
Sbjct: 421 PKDSLSHELFLSLLPPSMVARATHGSLGNSNSLRNKIQEFLLSDDDAES 467

[Close]

BLAST of CSPI03G37350 vs. ExPASy TrEMBL
Match: A0A5D3DPK4 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006240 PE=3 SV=1)

HSP 1 Score: 864.8 bits (2233), Expect = 1.7e-247
Identity = 441/469 (94.03%), Postives = 451/469 (96.16%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDEHTIEQDSPPSSRP T A  E+R  IT DDNSNTNLCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGESR--ITNDDNSNTNLCFKCKS 60

Query: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMSTSGVGDDGR CSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180
           VHE+QVKAH NFEASRDRSLQVFGVGVAYINETAA SIPSDEIDKT+QTIQSVVSSL QP
Sbjct: 121 VHEIQVKAHKNFEASRDRSLQVFGVGVAYINETAAYSIPSDEIDKTIQTIQSVVSSLSQP 180

Query: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RK+LHIV IE+VYSSNL+DGREKLKKLLDAV+DVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKDLHIVPIENVYSSNLSDGREKLKKLLDAVDDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD 300
           LLVGTCTSRIACHVISSTVKGQGYSLP DVQYIDARWEVPVVLPLLDCLAQELNMLCC+D
Sbjct: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCCID 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN
Sbjct: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHAGSSILGACCSSCQFQIL 420
           VPLATKRRQ+RNNFKHVNSLSSESFCAVCNGPLNTSDV TRHA SSILGACCSSCQFQIL
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHASSSILGACCSSCQFQIL 420

Query: 421 PKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES 470
           PKDS S ELFLSLLPPSMVARATHGSLGNSNSLR KIQEFLLSDD+AES
Sbjct: 421 PKDSLSHELFLSLLPPSMVARATHGSLGNSNSLRNKIQEFLLSDDDAES 467

[Close]

BLAST of CSPI03G37350 vs. ExPASy TrEMBL
Match: A0A6J1I684 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111470013 PE=3 SV=1)

HSP 1 Score: 757.7 bits (1955), Expect = 2.9e-215
Identity = 392/475 (82.53%), Postives = 418/475 (88.00%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQ DE T+EQDS  +++ D  AA       T  D+ +T+LCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAA-------TYGDSGDTHLCFKCKA 60

Query: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMS+S  GDDGRFCSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180
           VHEMQVKAH NFEASRDRSL VFGVGVAYI+ET+AC IP  EID+T+QTIQSVVSSL  P
Sbjct: 121 VHEMQVKAHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
            K+LHIVQIESVYSSNL+DGREKLKKLLD V+DVTGKEDLIS+LRMLSLQKIAAENGYNR
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD 300
           L++GTCTSRIACHVISSTVKGQGYSLP D+QY+DARWEVPV LPL DCLAQELNMLCC D
Sbjct: 241 LVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKTVELTK S SGINDLVSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDLNESN 
Sbjct: 301 GLKTVELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNG 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHA------GSSILGACCSS 420
           VPLATKRRQ+RNNFKHVNSLSSE+FCAVCNGPLN SDV T  +       S + G CCSS
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSS 420

Query: 421 CQFQILPKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES 470
           CQFQILPKDS SQEL LSLLPPSMVARA HGS  NSNSLR+KIQEFLLSDDE ES
Sbjct: 421 CQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 468

[Close]

BLAST of CSPI03G37350 vs. ExPASy TrEMBL
Match: A0A6J1FHP7 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111445447 PE=3 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 6.5e-215
Identity = 391/475 (82.32%), Postives = 418/475 (88.00%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQ DE T+EQDS  +++ D  AA       T  D+ +T+LCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 60

Query: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMS+S  GDDGRFCSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180
           VHEMQVKAH NFEASRDRSLQVFGVGVAYI+ET+AC IP  EID+T+QTIQSVVSSL  P
Sbjct: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
            K+LHIVQIESVYSSNL+DGREKLKKLLD V+DVTGKEDLIS+LRMLSLQKIAAENGYNR
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD 300
           L++GTCTSRIACHVISSTVKGQGYSLP D+QY+DARWEVPV LPL DCLAQELNMLCC D
Sbjct: 241 LVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKT ELTK S SGINDLVSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDLNESN 
Sbjct: 301 GLKTEELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNG 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHA------GSSILGACCSS 420
           VPLATKRRQ+RNNFKHVNSLSSE+FCAVCNGPLN SDV T  +       S + G CCSS
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSS 420

Query: 421 CQFQILPKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES 470
           CQFQILPKDS SQEL LSLLPPSMVARA HGS  NSNSLR++IQEFLLSDDE ES
Sbjct: 421 CQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 468

[Close]

BLAST of CSPI03G37350 vs. NCBI nr
Match: XP_004136614.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucumis sativus] >XP_011652154.1 cytoplasmic tRNA 2-thiolation protein 2 [Cucumis sativus] >XP_031737791.1 cytoplasmic tRNA 2-thiolation protein 2 [Cucumis sativus] >KGN59393.1 hypothetical protein Csa_001562 [Cucumis sativus])

HSP 1 Score: 926.8 bits (2394), Expect = 7.5e-266
Identity = 469/469 (100.00%), Postives = 469/469 (100.00%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60

Query: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180
           VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP
Sbjct: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180

Query: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD 300
           LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD
Sbjct: 241 LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN
Sbjct: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHAGSSILGACCSSCQFQIL 420
           VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHAGSSILGACCSSCQFQIL
Sbjct: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHAGSSILGACCSSCQFQIL 420

Query: 421 PKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES 470
           PKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES
Sbjct: 421 PKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES 469

[Close]

BLAST of CSPI03G37350 vs. NCBI nr
Match: XP_008443191.1 (PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo] >XP_008443192.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo] >KAA0053926.1 cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo var. makuwa] >TYK25478.1 cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 864.8 bits (2233), Expect = 3.5e-247
Identity = 441/469 (94.03%), Postives = 451/469 (96.16%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDEHTIEQDSPPSSRP T A  E+R  IT DDNSNTNLCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGESR--ITNDDNSNTNLCFKCKS 60

Query: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMSTSGVGDDGR CSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180
           VHE+QVKAH NFEASRDRSLQVFGVGVAYINETAA SIPSDEIDKT+QTIQSVVSSL QP
Sbjct: 121 VHEIQVKAHKNFEASRDRSLQVFGVGVAYINETAAYSIPSDEIDKTIQTIQSVVSSLSQP 180

Query: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RK+LHIV IE+VYSSNL+DGREKLKKLLDAV+DVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKDLHIVPIENVYSSNLSDGREKLKKLLDAVDDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD 300
           LLVGTCTSRIACHVISSTVKGQGYSLP DVQYIDARWEVPVVLPLLDCLAQELNMLCC+D
Sbjct: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCCID 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN
Sbjct: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHAGSSILGACCSSCQFQIL 420
           VPLATKRRQ+RNNFKHVNSLSSESFCAVCNGPLNTSDV TRHA SSILGACCSSCQFQIL
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHASSSILGACCSSCQFQIL 420

Query: 421 PKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES 470
           PKDS S ELFLSLLPPSMVARATHGSLGNSNSLR KIQEFLLSDD+AES
Sbjct: 421 PKDSLSHELFLSLLPPSMVARATHGSLGNSNSLRNKIQEFLLSDDDAES 467

[Close]

BLAST of CSPI03G37350 vs. NCBI nr
Match: XP_038905927.1 (cytoplasmic tRNA 2-thiolation protein 2 [Benincasa hispida])

HSP 1 Score: 820.1 bits (2117), Expect = 9.9e-234
Identity = 419/474 (88.40%), Postives = 442/474 (93.25%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDEHTIEQ SPPSSR DT AA+E+ +V T DD+S+T+LCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQVSPPSSRSDTAAAVESCQVDTNDDSSHTHLCFKCKS 60

Query: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KER+S+SGVGDDGR CSDCFRSNLF KFRQAV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERISSSGVGDDGRLCSDCFRSNLFGKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180
           VHEMQVKAH NFEAS+DRSLQVFGVGVAYINETAA SIPS EID+T+QTIQSVVSSLC P
Sbjct: 121 VHEMQVKAHKNFEASKDRSLQVFGVGVAYINETAAYSIPSHEIDQTIQTIQSVVSSLCPP 180

Query: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RK+LHIVQIE+VYSSNL+DGREKLKKLLD VNDVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKDLHIVQIENVYSSNLSDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD 300
           LLVGTCTSRIACHVISSTVKGQGYSLP D+QYIDARWE+PVVLPLLDCLAQELN+LCCVD
Sbjct: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADIQYIDARWEIPVVLPLLDCLAQELNLLCCVD 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKTVELTK+SSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDL ES+N
Sbjct: 301 GLKTVELTKQSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLTESSN 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLT------RHAGSSILG-ACCS 420
           VPLATKRRQ+RNNFKHVNS+SSESFCAVCNGPLNTSDV T          SSILG  CCS
Sbjct: 361 VPLATKRRQKRNNFKHVNSISSESFCAVCNGPLNTSDVQTLRRFEAGQTSSSILGCTCCS 420

Query: 421 SCQFQILPKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEA 468
           SCQFQILPKDS SQELFLSLLPPSMVARA HGS GNSNSLR++IQEFLLSDDEA
Sbjct: 421 SCQFQILPKDSLSQELFLSLLPPSMVARAMHGSFGNSNSLREQIQEFLLSDDEA 474

[Close]

BLAST of CSPI03G37350 vs. NCBI nr
Match: XP_022971223.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita maxima])

HSP 1 Score: 757.7 bits (1955), Expect = 6.0e-215
Identity = 392/475 (82.53%), Postives = 418/475 (88.00%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQ DE T+EQDS  +++ D  AA       T  D+ +T+LCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAA-------TYGDSGDTHLCFKCKA 60

Query: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMS+S  GDDGRFCSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180
           VHEMQVKAH NFEASRDRSL VFGVGVAYI+ET+AC IP  EID+T+QTIQSVVSSL  P
Sbjct: 121 VHEMQVKAHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
            K+LHIVQIESVYSSNL+DGREKLKKLLD V+DVTGKEDLIS+LRMLSLQKIAAENGYNR
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD 300
           L++GTCTSRIACHVISSTVKGQGYSLP D+QY+DARWEVPV LPL DCLAQELNMLCC D
Sbjct: 241 LVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKTVELTK S SGINDLVSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDLNESN 
Sbjct: 301 GLKTVELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNG 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHA------GSSILGACCSS 420
           VPLATKRRQ+RNNFKHVNSLSSE+FCAVCNGPLN SDV T  +       S + G CCSS
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSS 420

Query: 421 CQFQILPKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES 470
           CQFQILPKDS SQEL LSLLPPSMVARA HGS  NSNSLR+KIQEFLLSDDE ES
Sbjct: 421 CQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 468

[Close]

BLAST of CSPI03G37350 vs. NCBI nr
Match: XP_022939604.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita moschata])

HSP 1 Score: 756.5 bits (1952), Expect = 1.3e-214
Identity = 391/475 (82.32%), Postives = 418/475 (88.00%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQ DE T+EQDS  +++ D  AA       T  D+ +T+LCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 60

Query: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMS+S  GDDGRFCSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180
           VHEMQVKAH NFEASRDRSLQVFGVGVAYI+ET+AC IP  EID+T+QTIQSVVSSL  P
Sbjct: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
            K+LHIVQIESVYSSNL+DGREKLKKLLD V+DVTGKEDLIS+LRMLSLQKIAAENGYNR
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD 300
           L++GTCTSRIACHVISSTVKGQGYSLP D+QY+DARWEVPV LPL DCLAQELNMLCC D
Sbjct: 241 LVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKT ELTK S SGINDLVSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDLNESN 
Sbjct: 301 GLKTEELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNG 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHA------GSSILGACCSS 420
           VPLATKRRQ+RNNFKHVNSLSSE+FCAVCNGPLN SDV T  +       S + G CCSS
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSS 420

Query: 421 CQFQILPKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES 470
           CQFQILPKDS SQEL LSLLPPSMVARA HGS  NSNSLR++IQEFLLSDDE ES
Sbjct: 421 CQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 468

[Close]

BLAST of CSPI03G37350 vs. TAIR 10
Match: AT4G35910.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 493.4 bits (1269), Expect = 2.0e-139
Identity = 260/473 (54.97%), Postives = 340/473 (71.88%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60
           MACNSS C+S CY  + D  +   D   S                    ++ ++C KCK 
Sbjct: 1   MACNSSGCESGCYDREKDNGSKIVDDAVSG-----------------GGNHESVCVKCKC 60

Query: 61  KERMSTSGVG-DDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQ 120
              M+    G DDGRFC+DCFR+N+F KFR AVTS+AMI+PSD VLVAFSGG  SRV+LQ
Sbjct: 61  NAPMTFGDGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQ 120

Query: 121 FVHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQ 180
           FVHE+Q+KA  N+EASRDRSL VFGVGVA+++ETAA    S E+   ++ ++  VS L  
Sbjct: 121 FVHELQIKALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSP 180

Query: 181 PRKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYN 240
           P K+LH+V +ES++ S+  D R++L KLLD+V D TGKEDL+ +L+MLSLQK+AAENGYN
Sbjct: 181 PAKDLHVVPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKVAAENGYN 240

Query: 241 RLLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCV 300
           RL++G+CTSRIA HV+++TVKG+GYSL  D+Q++DARW+VP+VLPL DC+  E+  LC +
Sbjct: 241 RLVLGSCTSRIASHVLTATVKGRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCLL 300

Query: 301 DGLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESN 360
           DGLKTVEL  +S  GINDLVSSFVALLQ ENPSRE TIVRTA KLTPF F++IP+ ++S 
Sbjct: 301 DGLKTVELACRSQCGINDLVSSFVALLQEENPSRECTIVRTAAKLTPFYFNKIPETDDS- 360

Query: 361 NVPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSD---VLTRHAGSS---ILGACCS 420
           NVP+AT+RR +R N K+  S+++E+FC +CNGPLN SD   + T   G     +  ACCS
Sbjct: 361 NVPMATQRRLKRFNLKYDGSMTTEAFCPICNGPLNRSDSSELDTFEEGQESDVLYAACCS 420

Query: 421 SCQFQILPKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDE 467
           SC+FQILP+D SS E F S LP  M+++  H  + +   LR+KI++ LL DDE
Sbjct: 421 SCRFQILPQDGSSLEQFSSFLPDHMISQVKHQKVDSQAYLREKIKDCLLLDDE 455

The following BLAST results are available for this feature:

BLAST of CSPI03G37350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65628	2.8e-138	54.97	Cytoplasmic tRNA 2-thiolation protein 2 OS=Arabidopsis thaliana OX=3702 GN=CTU2 ...	[more]
Q2QMW0	6.5e-103	48.75	Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. japonica OX=39947...	[more]
A2ZM73	1.4e-102	48.75	Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. indica OX=39946 G...	[more]
Q08B12	1.7e-23	25.86	Cytoplasmic tRNA 2-thiolation protein 2-A OS=Xenopus laevis OX=8355 GN=ctu2-a PE...	[more]
Q32NV1	1.3e-21	25.67	Cytoplasmic tRNA 2-thiolation protein 2-B OS=Xenopus laevis OX=8355 GN=ctu2-b PE...	[more]

BLAST of CSPI03G37350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LC57	3.6e-266	100.00	Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis sativus OX=3659 GN=Csa_3G8160...	[more]
A0A1S3B7H9	1.7e-247	94.03	Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo OX=3656 GN=LOC103486848 ...	[more]
A0A5D3DPK4	1.7e-247	94.03	Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A6J1I684	2.9e-215	82.53	Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111470...	[more]
A0A6J1FHP7	6.5e-215	82.32	Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of CSPI03G37350 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136614.1	7.5e-266	100.00	cytoplasmic tRNA 2-thiolation protein 2 [Cucumis sativus] >XP_011652154.1 cytopl...	[more]
XP_008443191.1	3.5e-247	94.03	PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo] >XP_008443192....	[more]
XP_038905927.1	9.9e-234	88.40	cytoplasmic tRNA 2-thiolation protein 2 [Benincasa hispida]	[more]
XP_022971223.1	6.0e-215	82.53	cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita maxima]	[more]
XP_022939604.1	1.3e-214	82.32	cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita moschata]	[more]

BLAST of CSPI03G37350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35910.1	2.0e-139	54.97	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 54..404 e-value: 1.7E-41 score: 144.3
IPR019407	Cytoplasmic tRNA 2-thiolation protein 2	PANTHER	PTHR20882	CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2	coord: 46..463
IPR019407	Cytoplasmic tRNA 2-thiolation protein 2	HAMAP	MF_03054	CTU2	coord: 49..423 score: 20.261314
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 78..305

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G37350.1	CSPI03G37350.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010311	lateral root formation
biological_process	GO:0032447	protein urmylation
biological_process	GO:0002143	tRNA wobble position uridine thiolation
biological_process	GO:0034227	tRNA thio-modification
biological_process	GO:0002098	tRNA wobble uridine modification
cellular_component	GO:0005829	cytosol
molecular_function	GO:0016779	nucleotidyltransferase activity
molecular_function	GO:0016783	sulfurtransferase activity
molecular_function	GO:0000049	tRNA binding