CSPI03G36650 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G36650
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	outer envelope pore protein 16-3, chloroplastic/mitochondrial
Location	Chr3: 31820986 .. 31826242 (+)
RNA-Seq Expression	CSPI03G36650
Synteny	CSPI03G36650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACATGAACGAGGGTATTATAGTAATTTACTAATCCATCTGGGAATCGAGTTGAAGAGAAGTTCTTCATCTTCATATGCGTTTAAGAAGAACTTCAGTCTCCTCTTTCCACAGAGCTTCATCAGAAAAAGGTACTTTTTCTCTTACCTTCAATCAAGTCCTTTGTGTTCATTGTTTTCCCCCGCCCTAATTTACAATACTTTACGACCTATCATGATCGTGTTCCATGTAATTGTTCACGCAATTCGTTTAGGTTTTGCCCCTCTCCGCTTGCTCTGATCTGCCCCCCATTTTATCACTGAAGATCATTTCCATGATTTTCTTTTCGAGCGATTTGAGAAATTTCATATTAATCTGCATTTGTAGCGGATATCGTTTGGTTTGATTTTGGAATTGTTTGTTATCTAGCATGTTATTTGATTCGAATTGGCGATGTACATTAGCATGTTTGCTAATAGCTGCTTTTATGTCACAGGATATTAGAAATTCGATAACGTAAAGGAATGGGATTAGGATATATATACATGTTTCGTTATTATTTCAACTAATTAATTTTCTGTTAATGTACATGATTTCATTATGTGGGCAGAAGAATTTAGGCCTGGGTCTTTGTGTTTCTTTTCTTTCCGCAGCTTTGGTCATCGTTTGTGACTTGAAAAAAAGAAAGGGAAACCCATGGATGCTTCAGAGCGTAGGTATTTGGAGGATGATGATACGTCGTTAATGAAAACGATTAAAGGGGCAACTATGGGATTTGTTTCTGGTACAATTTTTGGAACAATTGTTGCCACTTGGCATGATGTGCCCCGTGTTGAGAGGAATGTTGCTCTGCCTGGGCTTGTGAGAACCTTGAAAATTATGGGAAACTATGGGATGACGTTTGCTGCTATTGGAGGGGTATACATTGGCGTTGAGCAGCTTCTGCAGAATTATAGAATGAAGAGAGACTTCGTTAATGGGGCTGTAGGTGGTTTTACAGCTGGAGCATCTGTTCTTGGATATAAAGGTGAACTTTCTACCCTTTCTAGATCTGGATGTAGTTGCAGTTTGATTTTCATTAGCAAATGTGAAGTTATTGTGTAAGGGTTGTTTCTTCTTTTTCTCCACTTTTCTCTACCGAAGGATGGGATGAAGTTATATTTTATGAACGTGGAGAGATTTTTTTTGTCTAACTGCAGTTTATCTGGCATATTTGCACCTGTGGATGGGTGATAAGCAACCTCAAATTGATTCATGTTTCAGTATATAAATGCTAATTCCAAAAAAGTTAGCCCATTTATCATTTAGTTCACTCATTGTTGTTTTCCATCTAACCTCTTTGGTTTCCTTACTCTCAACCTTTTTATGAATGGTGCACTGCAGGAGGATTGAAAATATGTTTCTTAAGCTCGGTTAATTAGGAGTCTTTTCTACTGTGTTTTTTACATCCTTTTGGTGTTCTCTTTCTAAGTCTTTCTGTGATTATGATCTTGTGCATATTTTAGCTAATTAGAAGTCTTTTCTATAAACCCTTTGGCCTAGCGGGGATATCTCATCTCACCTTTTTGTATGACTTTCTTCCTACCAACATATTCCTTGTTCGAGGAAAAAATAGACAAATTATGAAAGATAACTGATATTTTAATGTTCAATACTGACACGATTACAGGCTAAGCAAGTGGAAGTAGCACTACGGCATAACTGTGTGAAACTGATACAGTTACTCATGAAACTTTAGAAGGTTTTGAACGCTCCCAACTAGCATTTCATTCCAATACAAAGACAACATCTACTTGCCACACCCCCTTTGCATCTATATTGACATCTAACTAGCATGCACTTAATGCAACAAAAACATAGAAAGACAAAAATGAAATTTCTTTCCTGCCCTGTTTCTCTCCTTTCTTTTATATTTTCTTCCTTCTGATGTTGAGTGATGGGTGTGTGACGTGATGACACGATGAAAAAGGAGGAAAATCCAAAAAAAGAAAAAAACAAGTGGAGAGGAGAGGAAGGGAGAGCTTTTGTGTTTGCTTCCTTCAACCATTTTCTCATTTTTCGTTGTTTTTACCTGGTTATTTTTTTTTCTCAGAAATCTATAGACCTCTGTTGTCCTTTCCTCCTTTCTGTTTGGCCTCTGGTTTGCCACCCCTTTTTTCTTGCTCGTTTGTTTCTTGTTTCTGTATTCATTTGACAGACAGATGTTAATGAGTTTTTCTGTGTCGTGGTTGTACTTTGTGTGCAACAGTCACTAGCCCACCCCCTCTTCTTTTGTTAGTGTTGACGGGCAACTTAGTGTTTCCCAATCTGTAGGTGAAGATATCCTCCTTGTTAATTCTAGCCTCTTGTAGTCATTATTAGTGAAAATAATTTTGATTTAAGGAGGTTTTTGTTAGGAACCAAGGTAGTATGGGATGCCTTTGTCCCACATTGGTTAGAATGGGATGACCACTGTGGGATGACCAATAGTTGGCTTTTGGGGTGTGGTTCTCCAAGTTTCTTAAGTACCTAACAGTTTTACTACATTTAATACAGTCGAAAAATTCTCCTTTATTTACAGTCTTGAGACATTTCATGGGGGGATCCAAAATGCAGTTCTTGGGTCAAGAACTGAAGAAGACGGTTCTCTTATTAGAGAATATCTTCCTCTTGTGAAGTAGAAGTGAGAGAAATTGTAGGGGCGTCTTATTTCTCTTCATCCAATTGATCCATTTTTAGTCCTCAAAATGAAAAAGAAGAAAGAAAAATTGAATGTTTCTACTAGTCCATTAAATTGGAGGAAATAGAAACTTTCTTTTCTTAGATGATGACTTCCTCTTAATTTTTGTTAATTTACTAAGTATTATGCTAGTTCTTATCATCTGTTAATTCTTATTAGCACTAATGATGTGGAACTAAATTGAAAATTGTTGAAACATCAGGGACTAAATTAAAAATTTTAGATAACAAAGCAAGCATAAGTAGTCAAGGAAATTATAGAAATGTTTTGGAGCTCTATTAATAGAGGTTGGGATGAAGTGAAGTTTTAGGAAGATCAGATGGTATTCTCACTCTTTGAAATGAAGATAAGGTGAAGGTAAAAGATGGCAGGATATCTTCAAGGAATCTTTTCTATTCATGTGAAAATAGTGTTGTAAGATGGAAAATAATGCCGAGTGTCTAAATTTTGAAGATAACTTTATTCAGGAAGAGATCTTGAATATTTTCAGCAAGTAGTATGGTAAGAATACAGAAGTGCTGATCATTCTCGAGATTTCTTAATCTCAAAGGATTATGGGAAGTGGAAACTCTAGCTCACCTATTCCTCCATTGTTATCCTTGATGGCTGAGAGACCACATGTAGAAGATTACATAGTGTATCATCGGAAAGTCGATATGTTGAAGATACTCCAAGATTTTTTGGGAGAGTCACCTCAATTTTTTTGTCTTATTAAAAGCAAAGTGCTGAAAGAGTGGTGGACTAACCAAGCTGTCTACCAAGTCGTTGCTCTAAGGTCTTGTCTAAGAGGTTAAACTGTCAGTTGCTTGACAAAATGTCAACAAGTCAAAATGCTTATACGGAAGGACCGCAACTAATTGATTCTATCTTTTTCTTACTTGGGCAATAAATCCACAACGCTAGAGTTTTAAAAGCTCTGGAAGGAAAATGAGTCCTGTATAAGTTAGAAAAATGGAAGCTTTACCTTTCTAATTGAGGAAATTTTCTCTTTGTCAAACAGTTCTTGCCACCTTACCTGCCTATATTTCATGTCAAGTTTTCCTATGCTTGGGGAAGTGGTGGTAAGTTTTGGAAAAAAAGTTGAAGATTTTTGTAGGGATGGTAATTCGGTTAGGAAGCTTAGCCACTTGTAAACTGTAATGTGGTGTTTTACAACTGGGTATGGCACTTCTTCAATGAATCAAATCAGTTTTGGCACAAGGTGTTCTCTAGCATTTTGAGGAGGAAGACGAGTGTTTACTTTTTGAGATTTCATTGTCCACTATATTTTATTGGAAAAAACTATTTAATCTGGATGCCTCTTGCCGGTAGTTCAATTCAGAATCATCATTTAGATTAGATTACAGTAAAAACGTAACATTGAACACCTATTAGAAGCATTCTGTAATTGCCAGCAAACAGAGTCTTGGAAAAAGGGGAAAATAGTTGTTTCCAGATGTCATTCAACTTCTCCCACCTAAACATGGAACCAAAGGCTGTGTTAGGATCCACAATATTTCTAAGCTTAGAAATGAGCAAGCAAGTGCTGCAGGACATTCTTTACACGTAGTGATACACCTGACTTATCCTTCCCATACTTGCTAGCAGTCTTTCTTTGCCATAGTCCATTGCTTTCTAGAAGGAACTACAACATGCATTTGGTTTAAAATATGTTGTTTCACTTGGCTATATTCATGAAGTCAAGACCTCTGTGTTCCAAAGAGCGAGAGACACAACACTGTACTTCATTAATAGACAACCTCCATCCTAGTGTGCACCTTCCTAAAGGATCCCCTCTCATATGTTCATCAAAGCTTCTACCAACTGAGCCTTAAAGACACAAATAATTATTCTGCACATTTGCAACAACCCCTTTGGCAGGGCAGAGCCATGAGTTGGGCACAAATAATTAAATGTTTTGGATATTGTTTGTTTGTTATATACTTATATTTACCACAAAAATCCCTACCAACACATGATTATAAAGTAAAATGGCATTTTCATCTGTCTGATCCATTGCTCTAGGATGGCTATACTCTGTTTTCTTTTAATCTCTAATAAGTTAACTTGCTCTGAGAAGTTGTTGGGTTAATTGTTGTTATTGTTTTCTTTCAGGAAAAAGCATTTCAACAGCTTTATCCGCTGGAGCTGCTTTGGCATTTACTTCTTCAGTAATTGACATTGGTGGTCAAACCACAAGAATTGATACTGGCAAGGAGTATTATCCTTACACCACCAAGAAAAGATCCCACATTGAGTAGAATTAGGGAACTTTGCAGCAATTGGAAGAGAAGAAGTTGGTTGGCTTTCTCTTTTGGTTTTCAAGAGATGTAATTATACTCTCCGTTTCTCTTTGAGTGAGCTTAACAGGGAGATGGGTTGTATGAATAAAAGGTACTATCAATTTGTTTAACTTGTTTTTGGTTAGTTTTGGACTTACCACCATTGATGCAAGTTGGAAGCCTTTTTATTAAGCAATTTGGGAATATGAATATCAAGGTGGTTGTGAGAAATTGTCAATGACACTCTTTTTTTCCCTTCTTTTTCCAGGGTAATTTTATTTCTCAATTTAAATTCAAG

mRNA sequence

AAACATGAACGAGGGTATTATAGTAATTTACTAATCCATCTGGGAATCGAGTTGAAGAGAAGTTCTTCATCTTCATATGCGTTTAAGAAGAACTTCAGTCTCCTCTTTCCACAGAGCTTCATCAGAAAAAGCTTTGGTCATCGTTTGTGACTTGAAAAAAAGAAAGGGAAACCCATGGATGCTTCAGAGCGTAGGTATTTGGAGGATGATGATACGTCGTTAATGAAAACGATTAAAGGGGCAACTATGGGATTTGTTTCTGGTACAATTTTTGGAACAATTGTTGCCACTTGGCATGATGTGCCCCGTGTTGAGAGGAATGTTGCTCTGCCTGGGCTTGTGAGAACCTTGAAAATTATGGGAAACTATGGGATGACGTTTGCTGCTATTGGAGGGGTATACATTGGCGTTGAGCAGCTTCTGCAGAATTATAGAATGAAGAGAGACTTCGTTAATGGGGCTGTAGGTGGTTTTACAGCTGGAGCATCTGTTCTTGGATATAAAGGAAAAAGCATTTCAACAGCTTTATCCGCTGGAGCTGCTTTGGCATTTACTTCTTCAGTAATTGACATTGGTGGTCAAACCACAAGAATTGATACTGGCAAGGAGTATTATCCTTACACCACCAAGAAAAGATCCCACATTGAGTAGAATTAGGGAACTTTGCAGCAATTGGAAGAGAAGAAGTTGGTTGGCTTTCTCTTTTGGTTTTCAAGAGATGTAATTATACTCTCCGTTTCTCTTTGAGTGAGCTTAACAGGGAGATGGGTTGTATGAATAAAAGGTACTATCAATTTGTTTAACTTGTTTTTGGTTAGTTTTGGACTTACCACCATTGATGCAAGTTGGAAGCCTTTTTATTAAGCAATTTGGGAATATGAATATCAAGGTGGTTGTGAGAAATTGTCAATGACACTCTTTTTTTCCCTTCTTTTTCCAGGGTAATTTTATTTCTCAATTTAAATTCAAG

Coding sequence (CDS)

ATGGATGCTTCAGAGCGTAGGTATTTGGAGGATGATGATACGTCGTTAATGAAAACGATTAAAGGGGCAACTATGGGATTTGTTTCTGGTACAATTTTTGGAACAATTGTTGCCACTTGGCATGATGTGCCCCGTGTTGAGAGGAATGTTGCTCTGCCTGGGCTTGTGAGAACCTTGAAAATTATGGGAAACTATGGGATGACGTTTGCTGCTATTGGAGGGGTATACATTGGCGTTGAGCAGCTTCTGCAGAATTATAGAATGAAGAGAGACTTCGTTAATGGGGCTGTAGGTGGTTTTACAGCTGGAGCATCTGTTCTTGGATATAAAGGAAAAAGCATTTCAACAGCTTTATCCGCTGGAGCTGCTTTGGCATTTACTTCTTCAGTAATTGACATTGGTGGTCAAACCACAAGAATTGATACTGGCAAGGAGTATTATCCTTACACCACCAAGAAAAGATCCCACATTGAGTAG

Protein sequence

MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLKIMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSAGAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE*

Homology

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BLAST of CSPI03G36650 vs. ExPASy Swiss-Prot
Match: O48528 (Outer envelope pore protein 16-3, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OEP163 PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 1.6e-61
Identity = 112/158 (70.89%), Postives = 137/158 (86.71%), Query Frame = 0


Query: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60
           MD +E RYLE++D  LMKTIKG+  GF +GTI+GTI+ATW DVPRVERNVALPGL+RTLK
Sbjct: 1   MDPAEMRYLEEEDGPLMKTIKGSITGFGAGTIYGTILATWKDVPRVERNVALPGLIRTLK 60

Query: 61  IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA 120
           +MG +G+TFAAIGGVYIGVEQL+QN+R KRDF NGA+GGF AGASVLGY+ +SI TA++A
Sbjct: 61  MMGTHGLTFAAIGGVYIGVEQLVQNFRSKRDFYNGAIGGFVAGASVLGYRARSIPTAIAA 120

Query: 121 GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 159
           GA LA TS++ID GGQTTR+D G+EYYPYT +KR+  +
Sbjct: 121 GATLAVTSALIDSGGQTTRVDNGREYYPYTVEKRAEAD 158

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BLAST of CSPI03G36650 vs. ExPASy Swiss-Prot
Match: P0CR89 (Mitochondrial import inner membrane translocase subunit TIM22 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=TIM22 PE=3 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 8.9e-04
Identity = 28/71 (39.44%), Postives = 34/71 (47.89%), Query Frame = 0


Query: 62  MGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSAG 121
           M + G  FA +G VY GVE  ++ YR K D  NG   GF  GA +    G   +  L  G
Sbjct: 108 MWSSGRGFAKVGMVYSGVECCIEGYRAKNDIYNGVSAGFLTGAILARNAGP--TAMLGGG 167

Query: 122 AALAFTSSVID 133
            A A  S  ID
Sbjct: 168 VAFAAFSGAID 176

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BLAST of CSPI03G36650 vs. ExPASy Swiss-Prot
Match: P0CR88 (Mitochondrial import inner membrane translocase subunit TIM22 OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=TIM22 PE=3 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 8.9e-04
Identity = 28/71 (39.44%), Postives = 34/71 (47.89%), Query Frame = 0


Query: 62  MGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSAG 121
           M + G  FA +G VY GVE  ++ YR K D  NG   GF  GA +    G   +  L  G
Sbjct: 108 MWSSGRGFAKVGMVYSGVECCIEGYRAKNDIYNGVSAGFLTGAILARNAGP--TAMLGGG 167

Query: 122 AALAFTSSVID 133
            A A  S  ID
Sbjct: 168 VAFAAFSGAID 176

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BLAST of CSPI03G36650 vs. ExPASy TrEMBL
Match: A0A0A0LBC6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G809960 PE=4 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 6.4e-82
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0


Query: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60

Query: 61  IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA 120
           IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA
Sbjct: 61  IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA 120

Query: 121 GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 159
           GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE
Sbjct: 121 GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 158

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BLAST of CSPI03G36650 vs. ExPASy TrEMBL
Match: A0A5A7TNR7 (Outer envelope pore protein 16-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005590 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.0e-79
Identity = 153/158 (96.84%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATW+DVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWYDVPRVERNVALPGLVRTLK 60

Query: 61  IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA 120
           +MGNYGMTFAAIGGVYIGVEQL+QNYRMKRDFVNGAVGGF AGASVLGYKGKSISTALSA
Sbjct: 61  LMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAVGGFAAGASVLGYKGKSISTALSA 120

Query: 121 GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 159
           GAALAFTS+VIDIGGQTTRIDTGKEYYPYTTKKRSHIE
Sbjct: 121 GAALAFTSAVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 158

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BLAST of CSPI03G36650 vs. ExPASy TrEMBL
Match: A0A1S3B800 (outer envelope pore protein 16-3, chloroplastic/mitochondrial OS=Cucumis melo OX=3656 GN=LOC103486783 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.0e-79
Identity = 153/158 (96.84%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATW+DVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWYDVPRVERNVALPGLVRTLK 60

Query: 61  IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA 120
           +MGNYGMTFAAIGGVYIGVEQL+QNYRMKRDFVNGAVGGF AGASVLGYKGKSISTALSA
Sbjct: 61  LMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAVGGFAAGASVLGYKGKSISTALSA 120

Query: 121 GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 159
           GAALAFTS+VIDIGGQTTRIDTGKEYYPYTTKKRSHIE
Sbjct: 121 GAALAFTSAVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 158

[Close]

BLAST of CSPI03G36650 vs. ExPASy TrEMBL
Match: A0A6J1I3G7 (outer envelope pore protein 16-3, chloroplastic/mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111470193 PE=4 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 1.2e-75
Identity = 144/158 (91.14%), Postives = 152/158 (96.20%), Query Frame = 0


Query: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDTS+MKTIKGAT GFVSGTIFGTI ATW+DVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTSIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60

Query: 61  IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA 120
           +MGNYGMTFAAIGGVYIGVEQL+QNYRMKRDFVNGAVGGF AGASVLGYKGKSISTA+SA
Sbjct: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAVGGFAAGASVLGYKGKSISTAISA 120

Query: 121 GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 159
           G ALA TS++ID+GGQTTRID GKEYYPYTTKKRSHIE
Sbjct: 121 GVALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 158

[Close]

BLAST of CSPI03G36650 vs. ExPASy TrEMBL
Match: A0A6J1J6G7 (outer envelope pore protein 16-3, chloroplastic/mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111481780 PE=4 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 2.0e-75
Identity = 143/158 (90.51%), Postives = 152/158 (96.20%), Query Frame = 0


Query: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDTS+MKTIKGAT GFVSGTIFGTI ATW+DVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTSIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60

Query: 61  IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA 120
           +MGNYGMTFAAIGGVYIGVEQL+QNYRMKRDFVNGA+GGF AGASVLGYKGKSI TA+SA
Sbjct: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120

Query: 121 GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 159
           GAALA TS++ID+GGQTTRID GKEYYPYTTKKRSHIE
Sbjct: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 158

[Close]

BLAST of CSPI03G36650 vs. NCBI nr
Match: XP_004136578.1 (outer envelope pore protein 16-3, chloroplastic/mitochondrial [Cucumis sativus] >XP_031739126.1 outer envelope pore protein 16-3, chloroplastic/mitochondrial [Cucumis sativus] >KGN59320.1 hypothetical protein Csa_001852 [Cucumis sativus])

HSP 1 Score: 313.2 bits (801), Expect = 1.3e-81
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0


Query: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60

Query: 61  IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA 120
           IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA
Sbjct: 61  IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA 120

Query: 121 GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 159
           GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE
Sbjct: 121 GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 158

[Close]

BLAST of CSPI03G36650 vs. NCBI nr
Match: XP_008443078.1 (PREDICTED: outer envelope pore protein 16-3, chloroplastic/mitochondrial [Cucumis melo] >KAA0043716.1 outer envelope pore protein 16-3 [Cucumis melo var. makuwa] >TYK25417.1 outer envelope pore protein 16-3 [Cucumis melo var. makuwa])

HSP 1 Score: 305.8 bits (782), Expect = 2.1e-79
Identity = 153/158 (96.84%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATW+DVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWYDVPRVERNVALPGLVRTLK 60

Query: 61  IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA 120
           +MGNYGMTFAAIGGVYIGVEQL+QNYRMKRDFVNGAVGGF AGASVLGYKGKSISTALSA
Sbjct: 61  LMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAVGGFAAGASVLGYKGKSISTALSA 120

Query: 121 GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 159
           GAALAFTS+VIDIGGQTTRIDTGKEYYPYTTKKRSHIE
Sbjct: 121 GAALAFTSAVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 158

[Close]

BLAST of CSPI03G36650 vs. NCBI nr
Match: XP_038906015.1 (outer envelope pore protein 16-3, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 294.3 bits (752), Expect = 6.4e-76
Identity = 145/158 (91.77%), Postives = 153/158 (96.84%), Query Frame = 0


Query: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDTSLMKTIKGAT GFV+GTIFGTI ATW+DVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTSLMKTIKGATTGFVAGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60

Query: 61  IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA 120
           +MGNYGMTFAAIGGVYIGVEQL+QNYRMKRDF+NGAVGGF AGASVLGYKGKSISTA+SA
Sbjct: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFINGAVGGFAAGASVLGYKGKSISTAISA 120

Query: 121 GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 159
           GAALA TS+VID+GGQTTRID GKEYYPYTTKKRSHIE
Sbjct: 121 GAALAVTSAVIDVGGQTTRIDNGKEYYPYTTKKRSHIE 158

[Close]

BLAST of CSPI03G36650 vs. NCBI nr
Match: XP_022971491.1 (outer envelope pore protein 16-3, chloroplastic/mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 292.4 bits (747), Expect = 2.4e-75
Identity = 144/158 (91.14%), Postives = 152/158 (96.20%), Query Frame = 0


Query: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDTS+MKTIKGAT GFVSGTIFGTI ATW+DVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTSIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60

Query: 61  IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA 120
           +MGNYGMTFAAIGGVYIGVEQL+QNYRMKRDFVNGAVGGF AGASVLGYKGKSISTA+SA
Sbjct: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAVGGFAAGASVLGYKGKSISTAISA 120

Query: 121 GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 159
           G ALA TS++ID+GGQTTRID GKEYYPYTTKKRSHIE
Sbjct: 121 GVALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 158

[Close]

BLAST of CSPI03G36650 vs. NCBI nr
Match: XP_022948275.1 (outer envelope pore protein 16-3, chloroplastic/mitochondrial-like [Cucurbita moschata] >XP_023540815.1 outer envelope pore protein 16-3, chloroplastic/mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 291.6 bits (745), Expect = 4.2e-75
Identity = 144/158 (91.14%), Postives = 152/158 (96.20%), Query Frame = 0


Query: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDTS+MK IKGAT GFVSGTIFGTI ATW+DVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTSIMKMIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60

Query: 61  IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA 120
           +MGNYGMTFAAIGGVYIGVEQL+QNYRMKRDFVNGAVGGF AGASVLGYKGKSISTA+SA
Sbjct: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAVGGFAAGASVLGYKGKSISTAISA 120

Query: 121 GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 159
           GAALA TS++ID+GGQTTRID GKEYYPYTTKKRSHIE
Sbjct: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 158

[Close]

BLAST of CSPI03G36650 vs. TAIR 10
Match: AT2G42210.1 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 236.9 bits (603), Expect = 1.1e-62
Identity = 112/158 (70.89%), Postives = 137/158 (86.71%), Query Frame = 0


Query: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60
           MD +E RYLE++D  LMKTIKG+  GF +GTI+GTI+ATW DVPRVERNVALPGL+RTLK
Sbjct: 1   MDPAEMRYLEEEDGPLMKTIKGSITGFGAGTIYGTILATWKDVPRVERNVALPGLIRTLK 60

Query: 61  IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA 120
           +MG +G+TFAAIGGVYIGVEQL+QN+R KRDF NGA+GGF AGASVLGY+ +SI TA++A
Sbjct: 61  MMGTHGLTFAAIGGVYIGVEQLVQNFRSKRDFYNGAIGGFVAGASVLGYRARSIPTAIAA 120

Query: 121 GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 159
           GA LA TS++ID GGQTTR+D G+EYYPYT +KR+  +
Sbjct: 121 GATLAVTSALIDSGGQTTRVDNGREYYPYTVEKRAEAD 158

[Close]

BLAST of CSPI03G36650 vs. TAIR 10
Match: AT2G42210.2 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 236.9 bits (603), Expect = 1.1e-62
Identity = 112/158 (70.89%), Postives = 137/158 (86.71%), Query Frame = 0


Query: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60
           MD +E RYLE++D  LMKTIKG+  GF +GTI+GTI+ATW DVPRVERNVALPGL+RTLK
Sbjct: 15  MDPAEMRYLEEEDGPLMKTIKGSITGFGAGTIYGTILATWKDVPRVERNVALPGLIRTLK 74

Query: 61  IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA 120
           +MG +G+TFAAIGGVYIGVEQL+QN+R KRDF NGA+GGF AGASVLGY+ +SI TA++A
Sbjct: 75  MMGTHGLTFAAIGGVYIGVEQLVQNFRSKRDFYNGAIGGFVAGASVLGYRARSIPTAIAA 134

Query: 121 GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 159
           GA LA TS++ID GGQTTR+D G+EYYPYT +KR+  +
Sbjct: 135 GATLAVTSALIDSGGQTTRVDNGREYYPYTVEKRAEAD 172

[Close]

BLAST of CSPI03G36650 vs. TAIR 10
Match: AT2G42210.3 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 236.9 bits (603), Expect = 1.1e-62
Identity = 112/158 (70.89%), Postives = 137/158 (86.71%), Query Frame = 0


Query: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60
           MD +E RYLE++D  LMKTIKG+  GF +GTI+GTI+ATW DVPRVERNVALPGL+RTLK
Sbjct: 1   MDPAEMRYLEEEDGPLMKTIKGSITGFGAGTIYGTILATWKDVPRVERNVALPGLIRTLK 60

Query: 61  IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA 120
           +MG +G+TFAAIGGVYIGVEQL+QN+R KRDF NGA+GGF AGASVLGY+ +SI TA++A
Sbjct: 61  MMGTHGLTFAAIGGVYIGVEQLVQNFRSKRDFYNGAIGGFVAGASVLGYRARSIPTAIAA 120

Query: 121 GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 159
           GA LA TS++ID GGQTTR+D G+EYYPYT +KR+  +
Sbjct: 121 GATLAVTSALIDSGGQTTRVDNGREYYPYTVEKRAEAD 158

[Close]

BLAST of CSPI03G36650 vs. TAIR 10
Match: AT2G42210.4 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 236.9 bits (603), Expect = 1.1e-62
Identity = 112/158 (70.89%), Postives = 137/158 (86.71%), Query Frame = 0


Query: 1   MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60
           MD +E RYLE++D  LMKTIKG+  GF +GTI+GTI+ATW DVPRVERNVALPGL+RTLK
Sbjct: 1   MDPAEMRYLEEEDGPLMKTIKGSITGFGAGTIYGTILATWKDVPRVERNVALPGLIRTLK 60

Query: 61  IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGAVGGFTAGASVLGYKGKSISTALSA 120
           +MG +G+TFAAIGGVYIGVEQL+QN+R KRDF NGA+GGF AGASVLGY+ +SI TA++A
Sbjct: 61  MMGTHGLTFAAIGGVYIGVEQLVQNFRSKRDFYNGAIGGFVAGASVLGYRARSIPTAIAA 120

Query: 121 GAALAFTSSVIDIGGQTTRIDTGKEYYPYTTKKRSHIE 159
           GA LA TS++ID GGQTTR+D G+EYYPYT +KR+  +
Sbjct: 121 GATLAVTSALIDSGGQTTRVDNGREYYPYTVEKRAEAD 158

[Close]

BLAST of CSPI03G36650 vs. TAIR 10
Match: AT3G57900.1 (unknown protein; Has 22 Blast hits to 22 proteins in 7 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 22; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 50.4 bits (119), Expect = 1.5e-06
Identity = 32/96 (33.33%), Postives = 42/96 (43.75%), Query Frame = 0


Query: 1  MDASERRYLEDDDTSLMKTIKGATMGFVSGTIFGTIVATWHDVPRVERNVALPGLVRTLK 60
          MD +E RYLE+++ ++MKT K A  GF +G                              
Sbjct: 1  MDPTEMRYLEEENGAMMKTFKDAVTGFATG------------------------------ 56

Query: 61 IMGNYGMTFAAIGGVYIGVEQLLQNYRMKRDFVNGA 97
                        V+IGVEQL+QN+R KRDF NGA
Sbjct: 61 ----------KTSLVFIGVEQLVQNFRAKRDFFNGA 56

The following BLAST results are available for this feature:

BLAST of CSPI03G36650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48528	1.6e-61	70.89	Outer envelope pore protein 16-3, chloroplastic/mitochondrial OS=Arabidopsis tha...	[more]
P0CR89	8.9e-04	39.44	Mitochondrial import inner membrane translocase subunit TIM22 OS=Cryptococcus ne...	[more]
P0CR88	8.9e-04	39.44	Mitochondrial import inner membrane translocase subunit TIM22 OS=Cryptococcus ne...	[more]

BLAST of CSPI03G36650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LBC6	6.4e-82	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G809960 PE=4 SV=1	[more]
A0A5A7TNR7	1.0e-79	96.84	Outer envelope pore protein 16-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3B800	1.0e-79	96.84	outer envelope pore protein 16-3, chloroplastic/mitochondrial OS=Cucumis melo OX...	[more]
A0A6J1I3G7	1.2e-75	91.14	outer envelope pore protein 16-3, chloroplastic/mitochondrial-like OS=Cucurbita ...	[more]
A0A6J1J6G7	2.0e-75	90.51	outer envelope pore protein 16-3, chloroplastic/mitochondrial-like OS=Cucurbita ...	[more]

BLAST of CSPI03G36650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136578.1	1.3e-81	100.00	outer envelope pore protein 16-3, chloroplastic/mitochondrial [Cucumis sativus] ...	[more]
XP_008443078.1	2.1e-79	96.84	PREDICTED: outer envelope pore protein 16-3, chloroplastic/mitochondrial [Cucumi...	[more]
XP_038906015.1	6.4e-76	91.77	outer envelope pore protein 16-3, chloroplastic/mitochondrial [Benincasa hispida...	[more]
XP_022971491.1	2.4e-75	91.14	outer envelope pore protein 16-3, chloroplastic/mitochondrial-like [Cucurbita ma...	[more]
XP_022948275.1	4.2e-75	91.14	outer envelope pore protein 16-3, chloroplastic/mitochondrial-like [Cucurbita mo...	[more]

BLAST of CSPI03G36650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42210.1	1.1e-62	70.89	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]
AT2G42210.2	1.1e-62	70.89	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]
AT2G42210.3	1.1e-62	70.89	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]
AT2G42210.4	1.1e-62	70.89	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]
AT3G57900.1	1.5e-06	33.33	unknown protein; Has 22 Blast hits to 22 proteins in 7 species: Archae - 0; Bact...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF02466	Tim17	coord: 17..127 e-value: 2.7E-14 score: 53.5
None	No IPR available	PANTHER	PTHR14110:SF18	OUTER ENVELOPE PORE PROTEIN 16-3, CHLOROPLASTIC/MITOCHONDRIAL	coord: 3..156
IPR039175	Mitochondrial import inner membrane translocase subunit TIM22	PANTHER	PTHR14110	MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22	coord: 3..156

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G36650.1	CSPI03G36650.1	mRNA
CSPI03G36650.2	CSPI03G36650.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045039	protein insertion into mitochondrial inner membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0042721	TIM22 mitochondrial import inner membrane insertion complex