CSPI03G33840 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G33840
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionDEAD-box ATP-dependent RNA helicase 38
LocationChr3: 29716480 .. 29719312 (+)
RNA-Seq ExpressionCSPI03G33840
SyntenyCSPI03G33840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCGTTCTCTGTTTCGCCCTCTCCAACACCCGCGTCAAGCTTACTATTTGATTCTTCACCTTCCCCATTGTGTACCAGCGATGGTAGATGATGCGGCTCCAGGTTCTACCACCAATGACTCGTACTGTGACGCCACCATCACTCATTCCCCGACTACTGCCCCATTCGTAGTTTCACCATTGCCAAAACGAGGTTGGGGCGATGAAGAACACCATGATGTGGTTGCTGATACTGGAGATTTATCCTCTGTGCCGTCCGAGGTCATGGAATCCCTTAAAATTGAAGACGAAACAACGCTTGAAGAACCCATAGACTCTAACATCACAGCTGTAAGTCTCTCACGTACTCCATCAAATGTGTTTAAATGGCGTCTAGGAATCAAGGACATAGGAAAAAGGCAATGAAATATGAATTTGAGGCGGAATCTTGTCATATTTTAGCTGATGAAACTTGTAGGGTTTAAGCGAGGATTAAGGATAGGATTTGGTTCTTTGTGTATCGGAATTAACATGATTGAAATTTCCGACCTGCATAGATATAAAGGAACTGTTTTTAGATCGTTGACAACCGGTGAAATTGTTTTGAGGTTTTAGTTTGTGTGCTCGGTTGTATAGCAATGCGGGGTTTCTTGTTATGCTTTTCGGTTAATTTATTTTTTATTTGATGTAGGTGACAACTGGTGATACTCCATATTCATCGGCTAGTACTTTTGAGGATTTGAATCTGTCTAAGGAGTTGTTGAAGGGTTTGTATGTTGAGATGAGATTCCATAAGCCAAGTAAAATTCAAGCTATAAGTTTGCCTATGATCCTGACTCCTCCTTACAAACATTTAATTGCTCAAGCACACAATGGTTCTGGAAAGACTACATGTTTTGTGCTTGGGATGTTGAGTCGTGTTGATGTGAACTTAAAGGCTCCTCAGGCATTTTGCATATGTCCTACAAGGGAGTTAGCAATGCAAGTAAGAAGCTCTCTTTTATGTTTAAATGAATGCCATTGGCTTCTATCTATGTTAGATCTTAATTCTTCTTGAATATTATTATTGTAGAATATTGAGGTTCTTAAGAAAATGGGAAAATACACCGGGATAACTTCAGAGTGTGCTGTTCCCGCTGACAGTGCAAACTATATGCCAGTATCAAAGCGGCCACCCACAACTGCACAAGTTGTGATTGGAACTCCTGGCACAATAAAGAAATGGATGTCAGGGAGGAAGTTGGGAGTAAGTTGCGTGAAGATTCTTGTTTTTGATGAGGCTGATCATATGCTTGGCGAGGTAAGTTACTATGTATAGACTGGACATATTGTAGCATCTGTGTCCATGGGAAGCAGTATCTTCTCTTGATCTGTTATTCAAGATCATAGTTTTGTAATGTATATTTTTGTATCATTAATGATCCGTTAAATGTATTAGTTATTTTGAGTTATTGTGAGGGTGGACAATGGTAGTTATTTTTTCTTTGTCTTTTGTTTTTATTCAGGATGGCTTTCAGGATGATTCATTGAGAATCATGAGGGACATTGAGAGAAGTAGCTCTCATTTCCAGGTTTGTCTATATTATCACATAACCATTTCTAATCATAGTAATCTCATTTTTCCTTTTCTTATTGTTAAAAACTTTGTTTCAGGTGCTTCTGTTCTCTGCTACATTCGATGAGAATGTTAAAAACTTTGTCTCTAGGGTGGTTAAAGACTACAACCAACTGTTTGTGAAGAAGGAAGAACTATCTTTGGAGAGTGTGAAGCAATTCAAGTTGATATGTCCAAATGAACAAACTAAAATTCGAGTCATAAAAGATAGAATTTTTGAACTAGCAGACAAATTGGGTCAAACAATTATATTTGTGGGCACAAGAAAGAGTGCTGGAGTGTTACACAAGGCACTAGTTGATCTTGGTTATGATGTGACTACCATCCAAGGGGCAATGACCAATGACATTAGAGACAAAATAATAAAAGAGTTCAAGGATGGTTTGACAAAAGTTCTTATTTCAACTGACCTTCTAGCTCGAGGCTTTGATCAGCAGCAAGTACGAATATCAGTTCTCCATCCACTCCTCTGTTTGCTGTGATAGTATATAACGTGATGCCTTTTCAGATCTCTAATATATGTTTATTTTAGGTCAATCTAGTTATCAATTATGATCTTCCTCTTAAACACGATCCTTCTTCACAAGCAATGAAATACAGATCTTCCTCATCATCAGAGCCTAACTTCGAGGTGTACCTCCATCGAATTGGTCGGGCAGGTCGTTTTGGGCGCAAGGGTAAGTTTCCGCTGCTATTGTACTTTCATATCTATGTTTCTAAATTCTTCCAGCCCTAAACCCTGGACATGCCTCCATGTTTCTAGATTGTTGATGCTAAGCAACTTTACAAGGGTTAGAAGCAATATTTTAAACTGGCTTCTTGGTTGTCCCGGACACTCATGGATATAAAAAATATTTATATAGATTGCGTCCATCACTTTTTTCTTGATTTGCAAACGCTTTTGTAGTATCATTTGCAATGAAGAGTTGCTAAACATACAATCTTTTGGGTATATTGTTAAATGAAAGCTCATGACTGTTGTGTGATCACTACAGGAGCTGTGTTTAACTTGCTCTGTGGCGATCAGGAAATCAAGCGAATGGACAAAATTCAGAAGCATTTTGGCTCTGAAGTAATAGAGGTAAGATTCCTTCTTTGGTTGACAACATTTGAATTCCACCCGTTTTCTGACCTATCTGGATATCGAATTTCTATCTCAGTACCTTTTGAAATGTATATTTCAGGTTAGAGACACTGATGAGGACATTGAAGCAGCCTTAAAATCTGCTGGATTGATTTGA

mRNA sequence

CCGTTCTCTGTTTCGCCCTCTCCAACACCCGCGTCAAGCTTACTATTTGATTCTTCACCTTCCCCATTGTGTACCAGCGATGGTAGATGATGCGGCTCCAGGTTCTACCACCAATGACTCGTACTGTGACGCCACCATCACTCATTCCCCGACTACTGCCCCATTCGTAGTTTCACCATTGCCAAAACGAGGTTGGGGCGATGAAGAACACCATGATGTGGTTGCTGATACTGGAGATTTATCCTCTGTGCCGTCCGAGGTCATGGAATCCCTTAAAATTGAAGACGAAACAACGCTTGAAGAACCCATAGACTCTAACATCACAGCTGTGACAACTGGTGATACTCCATATTCATCGGCTAGTACTTTTGAGGATTTGAATCTGTCTAAGGAGTTGTTGAAGGGTTTGTATGTTGAGATGAGATTCCATAAGCCAAGTAAAATTCAAGCTATAAGTTTGCCTATGATCCTGACTCCTCCTTACAAACATTTAATTGCTCAAGCACACAATGGTTCTGGAAAGACTACATGTTTTGTGCTTGGGATGTTGAGTCGTGTTGATGTGAACTTAAAGGCTCCTCAGGCATTTTGCATATGTCCTACAAGGGAGTTAGCAATGCAAAATATTGAGGTTCTTAAGAAAATGGGAAAATACACCGGGATAACTTCAGAGTGTGCTGTTCCCGCTGACAGTGCAAACTATATGCCAGTATCAAAGCGGCCACCCACAACTGCACAAGTTGTGATTGGAACTCCTGGCACAATAAAGAAATGGATGTCAGGGAGGAAGTTGGGAGTAAGTTGCGTGAAGATTCTTGTTTTTGATGAGGCTGATCATATGCTTGGCGAGGTAATAATCTCATTTTTCCTTTTCTTATTGTTAAAAACTTTGTTTCAGGTGCTTCTGTTCTCTGCTACATTCGATGAGAATGTTAAAAACTTTGTCTCTAGGGTGGTTAAAGACTACAACCAACTGTTTGTGAAGAAGGAAGAACTATCTTTGGAGAGTGTGAAGCAATTCAAGTTGATATGTCCAAATGAACAAACTAAAATTCGAGTCATAAAAGATAGAATTTTTGAACTAGCAGACAAATTGGGTCAAACAATTATATTTGTGGGCACAAGAAAGAGTGCTGGAGTGTTACACAAGGCACTAGTTGATCTTGGTTATGATGTGACTACCATCCAAGGGGCAATGACCAATGACATTAGAGACAAAATAATAAAAGAGTTCAAGGATGGTTTGACAAAAGTTCTTATTTCAACTGACCTTCTAGCTCGAGGCTTTGATCAGCAGCAAGTCAATCTAGTTATCAATTATGATCTTCCTCTTAAACACGATCCTTCTTCACAAGCAATGAAATACAGATCTTCCTCATCATCAGAGCCTAACTTCGAGGTGTACCTCCATCGAATTGGTCGGGCAGGTCGTTTTGGGCGCAAGGGAGCTGTGTTTAACTTGCTCTGTGGCGATCAGGAAATCAAGCGAATGGACAAAATTCAGAAGCATTTTGGCTCTGAAGTAATAGAGGTTAGAGACACTGATGAGGACATTGAAGCAGCCTTAAAATCTGCTGGATTGATTTGA

Coding sequence (CDS)

ATGGTAGATGATGCGGCTCCAGGTTCTACCACCAATGACTCGTACTGTGACGCCACCATCACTCATTCCCCGACTACTGCCCCATTCGTAGTTTCACCATTGCCAAAACGAGGTTGGGGCGATGAAGAACACCATGATGTGGTTGCTGATACTGGAGATTTATCCTCTGTGCCGTCCGAGGTCATGGAATCCCTTAAAATTGAAGACGAAACAACGCTTGAAGAACCCATAGACTCTAACATCACAGCTGTGACAACTGGTGATACTCCATATTCATCGGCTAGTACTTTTGAGGATTTGAATCTGTCTAAGGAGTTGTTGAAGGGTTTGTATGTTGAGATGAGATTCCATAAGCCAAGTAAAATTCAAGCTATAAGTTTGCCTATGATCCTGACTCCTCCTTACAAACATTTAATTGCTCAAGCACACAATGGTTCTGGAAAGACTACATGTTTTGTGCTTGGGATGTTGAGTCGTGTTGATGTGAACTTAAAGGCTCCTCAGGCATTTTGCATATGTCCTACAAGGGAGTTAGCAATGCAAAATATTGAGGTTCTTAAGAAAATGGGAAAATACACCGGGATAACTTCAGAGTGTGCTGTTCCCGCTGACAGTGCAAACTATATGCCAGTATCAAAGCGGCCACCCACAACTGCACAAGTTGTGATTGGAACTCCTGGCACAATAAAGAAATGGATGTCAGGGAGGAAGTTGGGAGTAAGTTGCGTGAAGATTCTTGTTTTTGATGAGGCTGATCATATGCTTGGCGAGGTAATAATCTCATTTTTCCTTTTCTTATTGTTAAAAACTTTGTTTCAGGTGCTTCTGTTCTCTGCTACATTCGATGAGAATGTTAAAAACTTTGTCTCTAGGGTGGTTAAAGACTACAACCAACTGTTTGTGAAGAAGGAAGAACTATCTTTGGAGAGTGTGAAGCAATTCAAGTTGATATGTCCAAATGAACAAACTAAAATTCGAGTCATAAAAGATAGAATTTTTGAACTAGCAGACAAATTGGGTCAAACAATTATATTTGTGGGCACAAGAAAGAGTGCTGGAGTGTTACACAAGGCACTAGTTGATCTTGGTTATGATGTGACTACCATCCAAGGGGCAATGACCAATGACATTAGAGACAAAATAATAAAAGAGTTCAAGGATGGTTTGACAAAAGTTCTTATTTCAACTGACCTTCTAGCTCGAGGCTTTGATCAGCAGCAAGTCAATCTAGTTATCAATTATGATCTTCCTCTTAAACACGATCCTTCTTCACAAGCAATGAAATACAGATCTTCCTCATCATCAGAGCCTAACTTCGAGGTGTACCTCCATCGAATTGGTCGGGCAGGTCGTTTTGGGCGCAAGGGAGCTGTGTTTAACTTGCTCTGTGGCGATCAGGAAATCAAGCGAATGGACAAAATTCAGAAGCATTTTGGCTCTGAAGTAATAGAGGTTAGAGACACTGATGAGGACATTGAAGCAGCCTTAAAATCTGCTGGATTGATTTGA

Protein sequence

MVDDAAPGSTTNDSYCDATITHSPTTAPFVVSPLPKRGWGDEEHHDVVADTGDLSSVPSEVMESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKIQAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQNIEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKLGVSCVKILVFDEADHMLGEVIISFFLFLLLKTLFQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVGTRKSAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVRDTDEDIEAALKSAGLI*
Homology
BLAST of CSPI03G33840 vs. ExPASy Swiss-Prot
Match: Q93ZG7 (DEAD-box ATP-dependent RNA helicase 38 OS=Arabidopsis thaliana OX=3702 GN=RH38 PE=1 SV=2)

HSP 1 Score: 535.4 bits (1378), Expect = 6.8e-151
Identity = 296/515 (57.48%), Postives = 377/515 (73.20%), Query Frame = 0

Query: 1   MVDDAAPGSTTNDSYCDATITHSPTTAPFVVSPLPKRGWGDEEHHDVVADTGDLSSVPSE 60
           M D      T  +S   +T+  S  +A        K+ WGD      V D  D     SE
Sbjct: 1   MADTVEKVPTVVESSSSSTVEAS-NSAEKTEPTTEKKKWGD------VEDDDDEEEAVSE 60

Query: 61  VMESLKIEDE----TTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRF 120
            + SL I++E    + LEEP DSNI AVT+GDTPY+SAS FEDLNLS EL+KGLYVEM+F
Sbjct: 61  -LNSLSIKEEEKPDSILEEPEDSNIKAVTSGDTPYTSASRFEDLNLSPELMKGLYVEMKF 120

Query: 121 HKPSKIQAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTR 180
            KPSKIQAISLPMI+TPP+KHLIAQAHNGSGKTTCFVLGMLSRVD  L+ PQA CICPTR
Sbjct: 121 EKPSKIQAISLPMIMTPPHKHLIAQAHNGSGKTTCFVLGMLSRVDPTLREPQALCICPTR 180

Query: 181 ELAMQNIEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGR 240
           ELA QN+EVL+KMGK+TGIT+E AVP  +      ++  P +A VVIGTPGT+KKWM+ +
Sbjct: 181 ELANQNMEVLQKMGKFTGITAELAVPDSTRGAPAATRGAPVSAHVVIGTPGTLKKWMAFK 240

Query: 241 KLGVSCVKILVFDEADHMLGEVIISFFLFLLLKTL------FQVLLFSATFDENVKNFVS 300
           +LG++ +KILVFDEADHML           ++K +      FQVLLFSATF+E VK+FV+
Sbjct: 241 RLGLNHLKILVFDEADHMLATDGFRDDSLKIMKDIGRVNPNFQVLLFSATFNETVKDFVA 300

Query: 301 RVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVGTRK 360
           R VKD NQLFVK+E+L+L+SVKQ+K++CP EQ KI VIKD+I EL D +GQTIIFV T+ 
Sbjct: 301 RTVKDPNQLFVKREDLALDSVKQYKVVCPKEQNKIEVIKDQIMELGD-IGQTIIFVKTKA 360

Query: 361 SAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNL 420
           SA  +HKAL ++GYDVT++ G +T   RDKI+KEFK+ LT+VLI+TD++ARGFDQQ+VNL
Sbjct: 361 SAQKVHKALAEMGYDVTSVHGNLTESDRDKIVKEFKECLTQVLIATDVIARGFDQQRVNL 420

Query: 421 VINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKR 480
           V+NY+LP K++            + EP++EVYLHR+GRAGRFGRKGAVFNLL  D   K 
Sbjct: 421 VVNYNLPTKYE------------TGEPDYEVYLHRVGRAGRFGRKGAVFNLLLDDGWDKE 480

Query: 481 -MDKIQKHFGSEVIEVR--DTDEDIEAALKSAGLI 503
            M+KI+K+F + V E++  +++E+ ++ALK AGL+
Sbjct: 481 VMEKIEKYFEANVKEIKSWNSEEEYKSALKEAGLL 494

BLAST of CSPI03G33840 vs. ExPASy Swiss-Prot
Match: Q10RI7 (DEAD-box ATP-dependent RNA helicase 38 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0158200 PE=3 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 1.1e-135
Identity = 259/438 (59.13%), Postives = 323/438 (73.74%), Query Frame = 0

Query: 73  LEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKIQAISLPMILT 132
           L++  +S I AVT+G T Y SA+ FEDL L+ ELLKGL+ EM F +PSKIQA++LPMILT
Sbjct: 78  LDDSDESQIQAVTSGGTVYESAAAFEDLKLTPELLKGLHDEMGFSRPSKIQAVTLPMILT 137

Query: 133 PPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQNIEVLKKMGKY 192
           PPYK LIAQAHNGSGKTTCFVLGMLSRVD N K  QA CICPTRELA QN  VL +MGK+
Sbjct: 138 PPYKDLIAQAHNGSGKTTCFVLGMLSRVDPNRKVTQAICICPTRELAQQNKSVLMRMGKF 197

Query: 193 TGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKLGVSCVKILVFDEAD 252
           TGIT  CA+P    +Y+P++K P  T QVVIGT GT+ KW++ +K+  + +KILVFDEAD
Sbjct: 198 TGITCACAIPPAQKDYVPIAKMPKITDQVVIGTSGTLMKWINHKKILTNDIKILVFDEAD 257

Query: 253 HMLGEVIISFFLFLLLKTL------FQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEEL 312
           HML E         +++ +       QVLLFSATF+E VK+FV+RV+KD NQ+FVKKEEL
Sbjct: 258 HMLAEDGFRSDSERIMRDIQRSAGGCQVLLFSATFNERVKDFVTRVIKDGNQIFVKKEEL 317

Query: 313 SLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVGTRKSAGVLHKALVDLGYDV 372
           +LE VKQ+K+  P+E+ KI VIKD+IFE   K+GQ IIFV T++S   +H AL    Y  
Sbjct: 318 TLEKVKQYKVQVPDERAKIAVIKDKIFEFGQKVGQVIIFVRTKQSTKDVHNALTLEDYVC 377

Query: 373 TTIQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQA 432
           ++IQG++    R+KII+EFK+G TKVLISTD+LARGFDQ QVNLVINYD+P+K       
Sbjct: 378 SSIQGSLDQSEREKIIQEFKNGYTKVLISTDVLARGFDQAQVNLVINYDMPIKF------ 437

Query: 433 MKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVR 492
                 +  EP++EVYLHRIGRAGRFGRKGAVFNLLCG+ +   M KI+ +F   V EVR
Sbjct: 438 -----GTRDEPDYEVYLHRIGRAGRFGRKGAVFNLLCGETDNTVMRKIETYFQHNVPEVR 497

Query: 493 D--TDEDIEAALKSAGLI 503
           +  ++ED E ALK AGL+
Sbjct: 498 NWQSEEDFERALKDAGLV 504

BLAST of CSPI03G33840 vs. ExPASy Swiss-Prot
Match: Q4P7Z8 (ATP-dependent RNA helicase DBP5 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=DBP5 PE=3 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.0e-73
Identity = 167/458 (36.46%), Postives = 266/458 (58.08%), Query Frame = 0

Query: 49  ADTGDLSSVPSEVMESLKIEDETTLEEPIDSNITAV---TTGDTPYSSASTFEDLNLSKE 108
           ++  +++S  S ++ S  + +    E   +  +T        ++P  SA +FE L L + 
Sbjct: 15  SNVNEIASGISGLLSSKDVAESNLRESSHEVQVTLADQQADPNSPLYSAKSFEALGLHEN 74

Query: 109 LLKGLYVEMRFHKPSKIQAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLK 168
           LLKG+Y  M++ KPSKIQ  +LP++L  P K++I Q+ +G+GKT  F+L MLSR+D +L+
Sbjct: 75  LLKGIYA-MKYQKPSKIQEKALPLLLQNPPKNMIGQSQSGTGKTAAFILTMLSRIDYDLQ 134

Query: 169 APQAFCICPTRELAMQNIEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGT 228
            PQA  + P+RELA Q ++V + M K+T +T+   +P +      V +    TAQ++IGT
Sbjct: 135 KPQAIALAPSRELARQIMDVARTMSKFTNVTTCLCLPDE------VKRGEKITAQLIIGT 194

Query: 229 PGTIKKWMSGRKLGVSCVKILVFDEADHMLGEVIISFFLFLLLKTL---FQVLLFSATFD 288
           PG     +  + +  + +K+ V DEAD+ML +  +      +  T+    Q++LFSATF 
Sbjct: 195 PGKTFDMIKSKGIDTAAIKVFVLDEADNMLDQQSLGEQSIRVKNTMPKSCQLVLFSATFP 254

Query: 289 ENVKNFVSRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQT 348
            NV +F  R+    N++ +K+EELS+E +KQF + C +E  K  V+ + ++ L   +GQ+
Sbjct: 255 TNVYDFAVRIAPGANEIRLKQEELSVEGIKQFYMDCKDEDHKYEVLVE-LYNLL-TIGQS 314

Query: 349 IIFVGTRKSAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARG 408
           IIF   R++A  + + +   G+ V ++ G +    RD+ I  F+DG +KVLIST+++ARG
Sbjct: 315 IIFCAKRETADRIAQKMTQEGHKVDSLHGRLETADRDRTIDAFRDGKSKVLISTNVIARG 374

Query: 409 FDQQQVNLVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLL 468
            D QQV LVINYD+PL             + + E + E YLHRIGR GRFGRKG   N +
Sbjct: 375 IDIQQVTLVINYDMPL-------------TQTGEADAETYLHRIGRTGRFGRKGVSINFV 434

Query: 469 CGDQEIKRMDKIQKHFGSEVIEVRDTD-EDIEAALKSA 500
              Q    MD+I+K    ++  V   D E++E  +K A
Sbjct: 435 HDQQSWSYMDQIEKALKCQITRVATNDLEEMEYTIKEA 450

BLAST of CSPI03G33840 vs. ExPASy Swiss-Prot
Match: Q6CJU1 (ATP-dependent RNA helicase DBP5 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=DBP5 PE=3 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 7.5e-73
Identity = 168/448 (37.50%), Postives = 267/448 (59.60%), Query Frame = 0

Query: 70  ETTLEEPIDSNITA-----------VTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHK 129
           ET  EEP +  I +               ++P  S  +FE+L LS+ELLKGLY  M+F K
Sbjct: 43  ETKAEEPANKVINSEYEVKVNLADLQADANSPLYSVKSFEELGLSEELLKGLYA-MKFQK 102

Query: 130 PSKIQAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTREL 189
           PSKIQ  +LP+++  P  ++IAQ+ +G+GKT  F L ML+RVD N+ + QA C+ P REL
Sbjct: 103 PSKIQEKALPLLIRDPPHNMIAQSQSGTGKTAAFSLTMLTRVDPNVNSTQAICLSPAREL 162

Query: 190 AMQNIEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKL 249
           A Q +EV+++MGK+T  +S+  VP DS       +  P TA +V+GTPGT+   +  + L
Sbjct: 163 ARQTLEVIQEMGKFTKTSSQLVVP-DS-----FERNKPITANIVVGTPGTVLDLIRRKML 222

Query: 250 GVSCVKILVFDEADHM-----LGEVIISFFLFLLLKTLFQVLLFSATFDENVKNFVSRVV 309
            +  +K+ V DEAD+M     LG+  I    F  L    Q++LFSATFD+ V+ +  +++
Sbjct: 223 NLGSIKVFVLDEADNMLDKQGLGDQCIRVKKF--LPKTCQLVLFSATFDDGVRQYAKKII 282

Query: 310 KDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVGTRKSAG 369
                L ++K E+++ ++KQ  + C NE+ K  ++ +    L   +G +IIFV T+++A 
Sbjct: 283 PTAVSLELQKNEVNVSAIKQLFMDCDNEEHKYTILSELYGLLT--IGSSIIFVKTKQTAN 342

Query: 370 VLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVIN 429
           +L+  L   G+ V+ + G + +  RD++I +F++G +KVLI+T++LARG D   V++V+N
Sbjct: 343 LLYAKLKKEGHQVSILHGDLQSQDRDRLIDDFREGRSKVLITTNVLARGIDIPSVSMVVN 402

Query: 430 YDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDK 489
           YDLP    P+ QA         +P+   Y+HRIGR GRFGR G   + +   +  + +  
Sbjct: 403 YDLPTL--PNGQA---------DPS--TYVHRIGRTGRFGRTGVAISFIHDKKSFEVLSA 462

Query: 490 IQKHFGS-EVIEVRDTD-EDIEAALKSA 500
           IQK+FG  E+ +V   D +++E  +K A
Sbjct: 463 IQKYFGDIEITKVPTDDLDEMETIVKKA 466

BLAST of CSPI03G33840 vs. ExPASy Swiss-Prot
Match: Q75C39 (ATP-dependent RNA helicase DBP5 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=DBP5 PE=3 SV=2)

HSP 1 Score: 275.8 bits (704), Expect = 9.8e-73
Identity = 168/453 (37.09%), Postives = 264/453 (58.28%), Query Frame = 0

Query: 46  DVVADTGDLSSVPSEVMESLKIEDETTLEEPIDSNITAV---TTGDTPYSSASTFEDLNL 105
           +   + G   S PS   E+    D   +    +  +         ++P  S  +FE+L L
Sbjct: 25  ETTLEAGSAESAPSAPAENQAKSDSNLINSEYEVKVRLADIQADPNSPLYSVKSFEELGL 84

Query: 106 SKELLKGLYVEMRFHKPSKIQAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDV 165
           + ELLKGLY  M+F KPSKIQ  +LP++L  P +++IAQ+ +G+GKT  F L MLSRVDV
Sbjct: 85  APELLKGLYA-MKFQKPSKIQERALPLLLHNPPRNMIAQSQSGTGKTAAFSLTMLSRVDV 144

Query: 166 NLKAPQAFCICPTRELAMQNIEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVV 225
            + A QA C+ P+RELA Q +EV+++MGK+T I S+  VP DS       K     A ++
Sbjct: 145 AVPATQAICLAPSRELARQTLEVIQEMGKFTKIASQLIVP-DS-----YEKNKAINAHII 204

Query: 226 IGTPGTIKKWMSGRKLGVSCVKILVFDEADHM-----LGEVIISFFLFLLLKTLFQVLLF 285
           +GTPGT+   M  + + +  VK  V DEAD+M     LG+  I    FL   T  Q++LF
Sbjct: 205 VGTPGTVLDLMRRKMIQLGKVKTFVLDEADNMLDKQGLGDQCIRVKKFLPKDT--QLVLF 264

Query: 286 SATFDENVKNFVSRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELAD 345
           SATFD++V+ +  RVV + N L +++ E+++ ++KQ  + C +E+ K  V+ D    L  
Sbjct: 265 SATFDDSVREYARRVVPNANSLELQRNEVNVSAIKQLFMDCNDERHKFTVLCDLYGLLT- 324

Query: 346 KLGQTIIFVGTRKSAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTKVLISTD 405
            +G +IIFV T+++A +L+  L   G+ V+ + G + +  RD++I +F++G +KVLI+T+
Sbjct: 325 -IGSSIIFVQTKQTANMLYTELKREGHQVSILHGDLQSADRDRLIGDFREGRSKVLITTN 384

Query: 406 LLARGFDQQQVNLVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGA 465
           +LARG D   V++V+NYDLP+             +++ +P+   Y+HRIGR GRFGR G 
Sbjct: 385 VLARGIDIPTVSMVVNYDLPM-------------TANGQPDPSTYVHRIGRTGRFGRTGV 444

Query: 466 VFNLLCGDQEIKRMDKIQKHFGSEVIEVRDTDE 491
             + +   +  + +  IQ +FG   I    TD+
Sbjct: 445 AISFIHDKKSYETLAAIQSYFGDIQITKVPTDD 453

BLAST of CSPI03G33840 vs. ExPASy TrEMBL
Match: A0A0A0LDT3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G746560 PE=4 SV=1)

HSP 1 Score: 952.2 bits (2460), Expect = 8.7e-274
Identity = 487/508 (95.87%), Postives = 493/508 (97.05%), Query Frame = 0

Query: 1   MVDDAAPGSTTNDSYCDATITHSPTTAPFVVSPLPKRGWGDEEHHDVVADTGDLSSVPSE 60
           MVDDAAPGSTTNDSYCDATITHSPTTAPFVVSPLPKRGWGDEEHHDVVADTGDLSSVPSE
Sbjct: 1   MVDDAAPGSTTNDSYCDATITHSPTTAPFVVSPLPKRGWGDEEHHDVVADTGDLSSVPSE 60

Query: 61  VMESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPS 120
           VMESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPS
Sbjct: 61  VMESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPS 120

Query: 121 KIQAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAM 180
           KIQAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAM
Sbjct: 121 KIQAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAM 180

Query: 181 QNIEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKLGV 240
           QNIEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKLGV
Sbjct: 181 QNIEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKLGV 240

Query: 241 SCVKILVFDEADHMLGEVIISFFLFLLLKTL------FQVLLFSATFDENVKNFVSRVVK 300
           SCVKILVFDEADHMLGE     +   +++ +      FQVLLFSATFDENVKNFVSRVVK
Sbjct: 241 SCVKILVFDEADHMLGEDGFQDYSLRIMRDIERSSSHFQVLLFSATFDENVKNFVSRVVK 300

Query: 301 DYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVGTRKSAGV 360
           DYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVGTRKSAGV
Sbjct: 301 DYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVGTRKSAGV 360

Query: 361 LHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVINY 420
           LHKALVDLGYDVTTI+GAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVINY
Sbjct: 361 LHKALVDLGYDVTTIKGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVINY 420

Query: 421 DLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDKI 480
           DLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDKI
Sbjct: 421 DLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDKI 480

Query: 481 QKHFGSEVIEVRDTDEDIEAALKSAGLI 503
           QKHFGSEVIEVRDTDEDIEAALKSAGLI
Sbjct: 481 QKHFGSEVIEVRDTDEDIEAALKSAGLI 508

BLAST of CSPI03G33840 vs. ExPASy TrEMBL
Match: A0A5A7TRB4 (DEAD-box ATP-dependent RNA helicase 38 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G002860 PE=4 SV=1)

HSP 1 Score: 910.6 bits (2352), Expect = 2.9e-261
Identity = 475/533 (89.12%), Postives = 484/533 (90.81%), Query Frame = 0

Query: 3   DDAAPGSTTNDSYCDATITHSPTTAPFVVSPLPKRGWGDEEHHDVVADTGDLSSVPSEVM 62
           DD AP ST+N S CDATITHSP+TAPFVVSP+PKR WG+EE  DVVADTG  SSVPSEVM
Sbjct: 4   DDVAPDSTSNGSSCDATITHSPSTAPFVVSPMPKRSWGNEEDDDVVADTGGSSSVPSEVM 63

Query: 63  ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI 122
           ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI
Sbjct: 64  ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI 123

Query: 123 QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQN 182
           QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQN
Sbjct: 124 QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQN 183

Query: 183 IEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKLGVSC 242
           IEVLKKMGKYTGITSECAVPADSANYMPVSKRPP TAQVVIGTPGTIKKWMSGRKLGVSC
Sbjct: 184 IEVLKKMGKYTGITSECAVPADSANYMPVSKRPPITAQVVIGTPGTIKKWMSGRKLGVSC 243

Query: 243 VKILVFDEADHMLGE----------------------------VIIS-----FFLFLLLK 302
           VKILVFDEADHMLGE                            + IS      F FL++K
Sbjct: 244 VKILVFDEADHMLGEDGFQDDSLRIMRDIERSSSHCQVCLYYYITISNHSNLIFPFLIVK 303

Query: 303 TLFQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIK 362
            LFQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIK
Sbjct: 304 NLFQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIK 363

Query: 363 DRIFELADKLGQTIIFVGTRKSAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGL 422
           DRIFELADKLGQTIIFV TR SAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGL
Sbjct: 364 DRIFELADKLGQTIIFVATRNSAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGL 423

Query: 423 TKVLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRA 482
           TKVLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRA
Sbjct: 424 TKVLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRA 483

Query: 483 GRFGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVRDTDEDIEAALKSAGLI 503
           GRFGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVRD+DEDI+AALKSAGLI
Sbjct: 484 GRFGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVRDSDEDIQAALKSAGLI 536

BLAST of CSPI03G33840 vs. ExPASy TrEMBL
Match: A0A1S3B601 (DEAD-box ATP-dependent RNA helicase 38 OS=Cucumis melo OX=3656 GN=LOC103486545 PE=4 SV=1)

HSP 1 Score: 904.8 bits (2337), Expect = 1.6e-259
Identity = 467/506 (92.29%), Postives = 477/506 (94.27%), Query Frame = 0

Query: 3   DDAAPGSTTNDSYCDATITHSPTTAPFVVSPLPKRGWGDEEHHDVVADTGDLSSVPSEVM 62
           DD AP ST+N S CDATITHSP+TAPFVVSP+PKR WG+EE  DVVADTG  SSVPSEVM
Sbjct: 4   DDVAPDSTSNGSSCDATITHSPSTAPFVVSPMPKRSWGNEEDDDVVADTGGSSSVPSEVM 63

Query: 63  ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI 122
           ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI
Sbjct: 64  ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI 123

Query: 123 QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQN 182
           QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQN
Sbjct: 124 QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQN 183

Query: 183 IEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKLGVSC 242
           IEVLKKMGKYTGITSECAVPADSANYMPVSKRPP TAQVVIGTPGTIKKWMSGRKLGVSC
Sbjct: 184 IEVLKKMGKYTGITSECAVPADSANYMPVSKRPPITAQVVIGTPGTIKKWMSGRKLGVSC 243

Query: 243 VKILVFDEADHMLGEVIISFFLFLLLKTL------FQVLLFSATFDENVKNFVSRVVKDY 302
           VKILVFDEADHMLGE         +++ +       QVLLFSATFDENVKNFVSRVVKDY
Sbjct: 244 VKILVFDEADHMLGEDGFQDDSLRIMRDIERSSSHCQVLLFSATFDENVKNFVSRVVKDY 303

Query: 303 NQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVGTRKSAGVLH 362
           NQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFV TR SAGVLH
Sbjct: 304 NQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVATRNSAGVLH 363

Query: 363 KALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVINYDL 422
           KALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVINYDL
Sbjct: 364 KALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVINYDL 423

Query: 423 PLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDKIQK 482
           PLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDKIQK
Sbjct: 424 PLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDKIQK 483

Query: 483 HFGSEVIEVRDTDEDIEAALKSAGLI 503
           HFGSEVIEVRD+DEDI+AALKSAGLI
Sbjct: 484 HFGSEVIEVRDSDEDIQAALKSAGLI 509

BLAST of CSPI03G33840 vs. ExPASy TrEMBL
Match: A0A5D3DNK6 (DEAD-box ATP-dependent RNA helicase 38 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G003240 PE=4 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 1.7e-253
Identity = 466/533 (87.43%), Postives = 476/533 (89.31%), Query Frame = 0

Query: 3   DDAAPGSTTNDSYCDATITHSPTTAPFVVSPLPKRGWGDEEHHDVVADTGDLSSVPSEVM 62
           DD AP ST+N S CDATITHSP+TAPFVVSP+PKR WG+EE  DVVADTG  SSVPSEVM
Sbjct: 4   DDVAPDSTSNGSSCDATITHSPSTAPFVVSPMPKRSWGNEEDDDVVADTGGSSSVPSEVM 63

Query: 63  ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI 122
           ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI
Sbjct: 64  ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI 123

Query: 123 QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQN 182
           QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQ 
Sbjct: 124 QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQ- 183

Query: 183 IEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKLGVSC 242
             V   +  YTGITSECAVPADSANYMPVSKRPP TAQVVIGTPGTIKKWMSGRKLGVSC
Sbjct: 184 --VRTSLLLYTGITSECAVPADSANYMPVSKRPPITAQVVIGTPGTIKKWMSGRKLGVSC 243

Query: 243 VKILVFDEADHMLGE----------------------------VIIS-----FFLFLLLK 302
           VKILVFDEADHMLGE                            + IS      F FL++K
Sbjct: 244 VKILVFDEADHMLGEDGFQDDSLRIMRDIERSSSHCQVCLYYYITISNHSNLIFPFLIVK 303

Query: 303 TLFQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIK 362
            LFQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIK
Sbjct: 304 NLFQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIK 363

Query: 363 DRIFELADKLGQTIIFVGTRKSAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGL 422
           DRIFELADKLGQTIIFV TR SAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGL
Sbjct: 364 DRIFELADKLGQTIIFVATRNSAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGL 423

Query: 423 TKVLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRA 482
           TKVLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRA
Sbjct: 424 TKVLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRA 483

Query: 483 GRFGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVRDTDEDIEAALKSAGLI 503
           GRFGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVRD+DEDI+AALKSAGLI
Sbjct: 484 GRFGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVRDSDEDIQAALKSAGLI 533

BLAST of CSPI03G33840 vs. ExPASy TrEMBL
Match: A0A1S4E419 (DEAD-box ATP-dependent RNA helicase 38-like OS=Cucumis melo OX=3656 GN=LOC103501340 PE=4 SV=1)

HSP 1 Score: 812.0 bits (2096), Expect = 1.4e-231
Identity = 423/509 (83.10%), Postives = 456/509 (89.59%), Query Frame = 0

Query: 1   MVDDAAPGSTTNDSYCDATITHSPTTAPFVV-SPLPKRGWGDEEHHDVVADTGDLSSVPS 60
           M D  AP ST++ S   A +T   ++AP VV +P+PKR WGDEE  DVV ++GD SS PS
Sbjct: 1   MADHTAPDSTSDVSAAAAAVTADSSSAPPVVTTPVPKRAWGDEEDDDVV-ESGDSSSAPS 60

Query: 61  EVMESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKP 120
           E +ESLKI+DET LEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEM+FHKP
Sbjct: 61  EYLESLKIQDETNLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMKFHKP 120

Query: 121 SKIQAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELA 180
           SKIQAISLPMILTPPYK LIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELA
Sbjct: 121 SKIQAISLPMILTPPYKDLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELA 180

Query: 181 MQNIEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKLG 240
           MQNIEVLKKMGKYTGITSECAVPADSANY+P+SKRPP TAQVVIGTPGTIKKWMS RKLG
Sbjct: 181 MQNIEVLKKMGKYTGITSECAVPADSANYIPMSKRPPITAQVVIGTPGTIKKWMSSRKLG 240

Query: 241 VSCVKILVFDEADHMLGEVIISFFLFLLLKTL------FQVLLFSATFDENVKNFVSRVV 300
           VSCVKILVFDEADHMLGE         +++ +       QVLLFSATFDENVKNFVSRVV
Sbjct: 241 VSCVKILVFDEADHMLGEDGFQDDSLRIMRDIERSSSHCQVLLFSATFDENVKNFVSRVV 300

Query: 301 KDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVGTRKSAG 360
           KDYNQLFVKKEELSLESVKQ+KLICP+E TKIRVIKDRIFELADKLGQTIIFV TR SAG
Sbjct: 301 KDYNQLFVKKEELSLESVKQYKLICPDELTKIRVIKDRIFELADKLGQTIIFVRTRNSAG 360

Query: 361 VLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVIN 420
           +LHKALVDLGY+VTTIQGA+T +IRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVIN
Sbjct: 361 MLHKALVDLGYEVTTIQGALTTEIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVIN 420

Query: 421 YDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDK 480
           YDLPLK++PS QA KYRSSS SEPN+EVYLHRIGRAGRFGRKGAVFNLLCGD+EI  MDK
Sbjct: 421 YDLPLKYEPSPQATKYRSSSLSEPNYEVYLHRIGRAGRFGRKGAVFNLLCGDEEIMLMDK 480

Query: 481 IQKHFGSEVIEVRDTDEDIEAALKSAGLI 503
           IQ HF SE+IEVRD+DEDI+AALK+AGL+
Sbjct: 481 IQNHFRSEIIEVRDSDEDIQAALKAAGLV 508

BLAST of CSPI03G33840 vs. NCBI nr
Match: XP_004137993.3 (DEAD-box ATP-dependent RNA helicase 38 [Cucumis sativus] >KAE8651061.1 hypothetical protein Csa_002116 [Cucumis sativus])

HSP 1 Score: 949.9 bits (2454), Expect = 8.9e-273
Identity = 487/508 (95.87%), Postives = 492/508 (96.85%), Query Frame = 0

Query: 1   MVDDAAPGSTTNDSYCDATITHSPTTAPFVVSPLPKRGWGDEEHHDVVADTGDLSSVPSE 60
           MVDDAAPGSTTNDSYCDATITHSPTTAPFVVSPLPKRGWGDEEHHDVVADTGDLSSVPSE
Sbjct: 1   MVDDAAPGSTTNDSYCDATITHSPTTAPFVVSPLPKRGWGDEEHHDVVADTGDLSSVPSE 60

Query: 61  VMESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPS 120
           VMESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPS
Sbjct: 61  VMESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPS 120

Query: 121 KIQAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAM 180
           KIQAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAM
Sbjct: 121 KIQAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAM 180

Query: 181 QNIEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKLGV 240
           QNIEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKLGV
Sbjct: 181 QNIEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKLGV 240

Query: 241 SCVKILVFDEADHMLGEVIISFFLFLLLKTL------FQVLLFSATFDENVKNFVSRVVK 300
           SCVKILVFDEADHMLGE         +++ +      FQVLLFSATFDENVKNFVSRVVK
Sbjct: 241 SCVKILVFDEADHMLGEDGFQDDSLRIMRDIERSSSHFQVLLFSATFDENVKNFVSRVVK 300

Query: 301 DYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVGTRKSAGV 360
           DYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVGTRKSAGV
Sbjct: 301 DYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVGTRKSAGV 360

Query: 361 LHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVINY 420
           LHKALVDLGYDVTTI+GAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVINY
Sbjct: 361 LHKALVDLGYDVTTIKGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVINY 420

Query: 421 DLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDKI 480
           DLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDKI
Sbjct: 421 DLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDKI 480

Query: 481 QKHFGSEVIEVRDTDEDIEAALKSAGLI 503
           QKHFGSEVIEVRDTDEDIEAALKSAGLI
Sbjct: 481 QKHFGSEVIEVRDTDEDIEAALKSAGLI 508

BLAST of CSPI03G33840 vs. NCBI nr
Match: KAA0043945.1 (DEAD-box ATP-dependent RNA helicase 38 [Cucumis melo var. makuwa])

HSP 1 Score: 910.6 bits (2352), Expect = 6.0e-261
Identity = 475/533 (89.12%), Postives = 484/533 (90.81%), Query Frame = 0

Query: 3   DDAAPGSTTNDSYCDATITHSPTTAPFVVSPLPKRGWGDEEHHDVVADTGDLSSVPSEVM 62
           DD AP ST+N S CDATITHSP+TAPFVVSP+PKR WG+EE  DVVADTG  SSVPSEVM
Sbjct: 4   DDVAPDSTSNGSSCDATITHSPSTAPFVVSPMPKRSWGNEEDDDVVADTGGSSSVPSEVM 63

Query: 63  ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI 122
           ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI
Sbjct: 64  ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI 123

Query: 123 QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQN 182
           QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQN
Sbjct: 124 QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQN 183

Query: 183 IEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKLGVSC 242
           IEVLKKMGKYTGITSECAVPADSANYMPVSKRPP TAQVVIGTPGTIKKWMSGRKLGVSC
Sbjct: 184 IEVLKKMGKYTGITSECAVPADSANYMPVSKRPPITAQVVIGTPGTIKKWMSGRKLGVSC 243

Query: 243 VKILVFDEADHMLGE----------------------------VIIS-----FFLFLLLK 302
           VKILVFDEADHMLGE                            + IS      F FL++K
Sbjct: 244 VKILVFDEADHMLGEDGFQDDSLRIMRDIERSSSHCQVCLYYYITISNHSNLIFPFLIVK 303

Query: 303 TLFQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIK 362
            LFQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIK
Sbjct: 304 NLFQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIK 363

Query: 363 DRIFELADKLGQTIIFVGTRKSAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGL 422
           DRIFELADKLGQTIIFV TR SAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGL
Sbjct: 364 DRIFELADKLGQTIIFVATRNSAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGL 423

Query: 423 TKVLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRA 482
           TKVLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRA
Sbjct: 424 TKVLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRA 483

Query: 483 GRFGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVRDTDEDIEAALKSAGLI 503
           GRFGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVRD+DEDI+AALKSAGLI
Sbjct: 484 GRFGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVRDSDEDIQAALKSAGLI 536

BLAST of CSPI03G33840 vs. NCBI nr
Match: XP_008442767.1 (PREDICTED: DEAD-box ATP-dependent RNA helicase 38 [Cucumis melo])

HSP 1 Score: 904.8 bits (2337), Expect = 3.3e-259
Identity = 467/506 (92.29%), Postives = 477/506 (94.27%), Query Frame = 0

Query: 3   DDAAPGSTTNDSYCDATITHSPTTAPFVVSPLPKRGWGDEEHHDVVADTGDLSSVPSEVM 62
           DD AP ST+N S CDATITHSP+TAPFVVSP+PKR WG+EE  DVVADTG  SSVPSEVM
Sbjct: 4   DDVAPDSTSNGSSCDATITHSPSTAPFVVSPMPKRSWGNEEDDDVVADTGGSSSVPSEVM 63

Query: 63  ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI 122
           ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI
Sbjct: 64  ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI 123

Query: 123 QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQN 182
           QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQN
Sbjct: 124 QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQN 183

Query: 183 IEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKLGVSC 242
           IEVLKKMGKYTGITSECAVPADSANYMPVSKRPP TAQVVIGTPGTIKKWMSGRKLGVSC
Sbjct: 184 IEVLKKMGKYTGITSECAVPADSANYMPVSKRPPITAQVVIGTPGTIKKWMSGRKLGVSC 243

Query: 243 VKILVFDEADHMLGEVIISFFLFLLLKTL------FQVLLFSATFDENVKNFVSRVVKDY 302
           VKILVFDEADHMLGE         +++ +       QVLLFSATFDENVKNFVSRVVKDY
Sbjct: 244 VKILVFDEADHMLGEDGFQDDSLRIMRDIERSSSHCQVLLFSATFDENVKNFVSRVVKDY 303

Query: 303 NQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVGTRKSAGVLH 362
           NQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFV TR SAGVLH
Sbjct: 304 NQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVATRNSAGVLH 363

Query: 363 KALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVINYDL 422
           KALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVINYDL
Sbjct: 364 KALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVINYDL 423

Query: 423 PLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDKIQK 482
           PLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDKIQK
Sbjct: 424 PLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDKIQK 483

Query: 483 HFGSEVIEVRDTDEDIEAALKSAGLI 503
           HFGSEVIEVRD+DEDI+AALKSAGLI
Sbjct: 484 HFGSEVIEVRDSDEDIQAALKSAGLI 509

BLAST of CSPI03G33840 vs. NCBI nr
Match: TYK25195.1 (DEAD-box ATP-dependent RNA helicase 38 [Cucumis melo var. makuwa])

HSP 1 Score: 884.8 bits (2285), Expect = 3.5e-253
Identity = 466/533 (87.43%), Postives = 476/533 (89.31%), Query Frame = 0

Query: 3   DDAAPGSTTNDSYCDATITHSPTTAPFVVSPLPKRGWGDEEHHDVVADTGDLSSVPSEVM 62
           DD AP ST+N S CDATITHSP+TAPFVVSP+PKR WG+EE  DVVADTG  SSVPSEVM
Sbjct: 4   DDVAPDSTSNGSSCDATITHSPSTAPFVVSPMPKRSWGNEEDDDVVADTGGSSSVPSEVM 63

Query: 63  ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI 122
           ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI
Sbjct: 64  ESLKIEDETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKI 123

Query: 123 QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQN 182
           QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQ 
Sbjct: 124 QAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQ- 183

Query: 183 IEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKLGVSC 242
             V   +  YTGITSECAVPADSANYMPVSKRPP TAQVVIGTPGTIKKWMSGRKLGVSC
Sbjct: 184 --VRTSLLLYTGITSECAVPADSANYMPVSKRPPITAQVVIGTPGTIKKWMSGRKLGVSC 243

Query: 243 VKILVFDEADHMLGE----------------------------VIIS-----FFLFLLLK 302
           VKILVFDEADHMLGE                            + IS      F FL++K
Sbjct: 244 VKILVFDEADHMLGEDGFQDDSLRIMRDIERSSSHCQVCLYYYITISNHSNLIFPFLIVK 303

Query: 303 TLFQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIK 362
            LFQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIK
Sbjct: 304 NLFQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIK 363

Query: 363 DRIFELADKLGQTIIFVGTRKSAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGL 422
           DRIFELADKLGQTIIFV TR SAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGL
Sbjct: 364 DRIFELADKLGQTIIFVATRNSAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGL 423

Query: 423 TKVLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRA 482
           TKVLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRA
Sbjct: 424 TKVLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRA 483

Query: 483 GRFGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVRDTDEDIEAALKSAGLI 503
           GRFGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVRD+DEDI+AALKSAGLI
Sbjct: 484 GRFGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVRDSDEDIQAALKSAGLI 533

BLAST of CSPI03G33840 vs. NCBI nr
Match: XP_038906287.1 (DEAD-box ATP-dependent RNA helicase 38 [Benincasa hispida] >XP_038906288.1 DEAD-box ATP-dependent RNA helicase 38 [Benincasa hispida] >XP_038906289.1 DEAD-box ATP-dependent RNA helicase 38 [Benincasa hispida])

HSP 1 Score: 828.2 bits (2138), Expect = 3.9e-236
Identity = 433/513 (84.41%), Postives = 461/513 (89.86%), Query Frame = 0

Query: 1   MVDDAAPGSTTND----SYCDATITHSPTTAPFVVSPLPKRGWGDEEHHDVVADTGDLSS 60
           M D AAP STT+D    +   AT T   ++AP +V+P+PKR WGDEE  D V DTGD SS
Sbjct: 1   MADHAAPDSTTSDASAPAAATATATDGSSSAPPIVTPVPKRAWGDEEDDD-VGDTGDSSS 60

Query: 61  VPSEVMESLKIE-DETTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMR 120
            PSE +ESLKIE DETTL+EPIDSNITAVTTG TPY+SASTFEDLNLSKELLKGLYVEMR
Sbjct: 61  APSEYLESLKIEDDETTLDEPIDSNITAVTTGGTPYTSASTFEDLNLSKELLKGLYVEMR 120

Query: 121 FHKPSKIQAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPT 180
           FHKPSKIQAISLPMILTPPYK LIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPT
Sbjct: 121 FHKPSKIQAISLPMILTPPYKDLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPT 180

Query: 181 RELAMQNIEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSG 240
           RELAMQNIEVLKKMGKYTGITSECAVPADSANY+P+SKRPP TAQVVIGTPGTIKKWMS 
Sbjct: 181 RELAMQNIEVLKKMGKYTGITSECAVPADSANYVPMSKRPPITAQVVIGTPGTIKKWMSS 240

Query: 241 RKLGVSCVKILVFDEADHMLGEVIISFFLFLLLKTL------FQVLLFSATFDENVKNFV 300
           RKLGVSCV+ILVFDEADHMLGE         ++K +       QVLLFSATFDENVK FV
Sbjct: 241 RKLGVSCVRILVFDEADHMLGEDGFQDDSLRIMKDIERSSPHCQVLLFSATFDENVKKFV 300

Query: 301 SRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVGTR 360
           SRVVKDYNQLFVKKEELSLESVKQFKLICP+E TKIRVIKDRIFELADKLGQTIIFV TR
Sbjct: 301 SRVVKDYNQLFVKKEELSLESVKQFKLICPDELTKIRVIKDRIFELADKLGQTIIFVRTR 360

Query: 361 KSAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVN 420
            SAG+LHKALVDLGYDVTTIQGA+TNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVN
Sbjct: 361 NSAGMLHKALVDLGYDVTTIQGALTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVN 420

Query: 421 LVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIK 480
           LVINYDLPLKH+PS QA KYRSSSSS+PN+EVYLHRIGRAGRFGRKGAVFNLLCGD+EI 
Sbjct: 421 LVINYDLPLKHEPSLQATKYRSSSSSDPNYEVYLHRIGRAGRFGRKGAVFNLLCGDEEIA 480

Query: 481 RMDKIQKHFGSEVIEVRDTDEDIEAALKSAGLI 503
           RMDKIQKHFGSE+IEVRD+DEDI+AALK+AGL+
Sbjct: 481 RMDKIQKHFGSEIIEVRDSDEDIQAALKTAGLV 512

BLAST of CSPI03G33840 vs. TAIR 10
Match: AT3G53110.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 535.4 bits (1378), Expect = 4.9e-152
Identity = 296/515 (57.48%), Postives = 377/515 (73.20%), Query Frame = 0

Query: 1   MVDDAAPGSTTNDSYCDATITHSPTTAPFVVSPLPKRGWGDEEHHDVVADTGDLSSVPSE 60
           M D      T  +S   +T+  S  +A        K+ WGD      V D  D     SE
Sbjct: 1   MADTVEKVPTVVESSSSSTVEAS-NSAEKTEPTTEKKKWGD------VEDDDDEEEAVSE 60

Query: 61  VMESLKIEDE----TTLEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRF 120
            + SL I++E    + LEEP DSNI AVT+GDTPY+SAS FEDLNLS EL+KGLYVEM+F
Sbjct: 61  -LNSLSIKEEEKPDSILEEPEDSNIKAVTSGDTPYTSASRFEDLNLSPELMKGLYVEMKF 120

Query: 121 HKPSKIQAISLPMILTPPYKHLIAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTR 180
            KPSKIQAISLPMI+TPP+KHLIAQAHNGSGKTTCFVLGMLSRVD  L+ PQA CICPTR
Sbjct: 121 EKPSKIQAISLPMIMTPPHKHLIAQAHNGSGKTTCFVLGMLSRVDPTLREPQALCICPTR 180

Query: 181 ELAMQNIEVLKKMGKYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGR 240
           ELA QN+EVL+KMGK+TGIT+E AVP  +      ++  P +A VVIGTPGT+KKWM+ +
Sbjct: 181 ELANQNMEVLQKMGKFTGITAELAVPDSTRGAPAATRGAPVSAHVVIGTPGTLKKWMAFK 240

Query: 241 KLGVSCVKILVFDEADHMLGEVIISFFLFLLLKTL------FQVLLFSATFDENVKNFVS 300
           +LG++ +KILVFDEADHML           ++K +      FQVLLFSATF+E VK+FV+
Sbjct: 241 RLGLNHLKILVFDEADHMLATDGFRDDSLKIMKDIGRVNPNFQVLLFSATFNETVKDFVA 300

Query: 301 RVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVGTRK 360
           R VKD NQLFVK+E+L+L+SVKQ+K++CP EQ KI VIKD+I EL D +GQTIIFV T+ 
Sbjct: 301 RTVKDPNQLFVKREDLALDSVKQYKVVCPKEQNKIEVIKDQIMELGD-IGQTIIFVKTKA 360

Query: 361 SAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNL 420
           SA  +HKAL ++GYDVT++ G +T   RDKI+KEFK+ LT+VLI+TD++ARGFDQQ+VNL
Sbjct: 361 SAQKVHKALAEMGYDVTSVHGNLTESDRDKIVKEFKECLTQVLIATDVIARGFDQQRVNL 420

Query: 421 VINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKR 480
           V+NY+LP K++            + EP++EVYLHR+GRAGRFGRKGAVFNLL  D   K 
Sbjct: 421 VVNYNLPTKYE------------TGEPDYEVYLHRVGRAGRFGRKGAVFNLLLDDGWDKE 480

Query: 481 -MDKIQKHFGSEVIEVR--DTDEDIEAALKSAGLI 503
            M+KI+K+F + V E++  +++E+ ++ALK AGL+
Sbjct: 481 VMEKIEKYFEANVKEIKSWNSEEEYKSALKEAGLL 494

BLAST of CSPI03G33840 vs. TAIR 10
Match: AT1G72730.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 206.8 bits (525), Expect = 4.0e-53
Identity = 141/401 (35.16%), Postives = 213/401 (53.12%), Query Frame = 0

Query: 96  TFEDLNLSKELLKGLYVEMRFHKPSKIQAISLPMILTPPYKHL--IAQAHNGSGKTTCFV 155
           +F+ + L  +LL+G+Y    F KPS IQ       + P  K L  I QA +G+GKT  F 
Sbjct: 42  SFDAMELQPDLLRGIYA-YGFEKPSAIQQRG----IIPFCKGLDVIQQAQSGTGKTATFC 101

Query: 156 LGMLSRVDVNLKAPQAFCICPTRELAMQNIEVLKKMGKYTGITSECAVPADSANYMPVSK 215
            G+L ++D++L   QA  + PTRELA Q  +V++ +G Y G+ ++  V   S       +
Sbjct: 102 SGVLQQLDISLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKAQACVGGTSVR--EDQR 161

Query: 216 RPPTTAQVVIGTPGTIKKWMSGRKLGVSCVKILVFDEADHMLGEVIIS--FFLFLLLKTL 275
              +   VV+GTPG +   +  + L    +K+ V DEAD ML        + +F LL + 
Sbjct: 162 VLQSGVHVVVGTPGRVFDLLRRQSLRADAIKMFVLDEADEMLSRGFKDQIYDIFQLLPSK 221

Query: 276 FQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDR 335
            QV +FSAT          + +    ++ VK++EL+LE +KQF +    E+ K+  + D 
Sbjct: 222 VQVGVFSATMPPEALEITRKFMNKPVRILVKRDELTLEGIKQFYVNVDKEEWKLETLCDL 281

Query: 336 IFELADKLGQTIIFVGTRKSAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTK 395
              LA  + Q++IFV TR+    L   +    + V+   G M  + RD I++EF+ G ++
Sbjct: 282 YETLA--ITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSR 341

Query: 396 VLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGR 455
           VLI+TDLLARG D QQV+LVIN+DLP                 ++P  E YLHRIGR+GR
Sbjct: 342 VLITTDLLARGIDVQQVSLVINFDLP-----------------TQP--ENYLHRIGRSGR 401

Query: 456 FGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVRDTDEDI 493
           FGRKG   N +  + E + M  IQ+ +   V E+     D+
Sbjct: 402 FGRKGVAINFMTSEDE-RMMADIQRFYNVVVEELPSNVADL 413

BLAST of CSPI03G33840 vs. TAIR 10
Match: AT1G54270.1 (eif4a-2 )

HSP 1 Score: 203.0 bits (515), Expect = 5.7e-52
Identity = 149/424 (35.14%), Postives = 214/424 (50.47%), Query Frame = 0

Query: 73  LEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKIQAISLPMILT 132
           L E +D      T+ D  + S   F+ + L + LL+G+Y    F KPS IQ       + 
Sbjct: 20  LNEVLDGQDEFFTSYDEVHES---FDAMGLQENLLRGIYA-YGFEKPSAIQQRG----IV 79

Query: 133 PPYKHL--IAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQNIEVLKKMG 192
           P  K L  I QA +G+GKT  F  G+L ++D  L   QA  + PTRELA Q  +V++ +G
Sbjct: 80  PFCKGLDVIQQAQSGTGKTATFCSGVLQQLDYALLQCQALVLAPTRELAQQIEKVMRALG 139

Query: 193 KYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKLGVSCVKILVFDE 252
            Y G+     V   S       +       VV+GTPG +   +  + L   C+K+ V DE
Sbjct: 140 DYQGVKVHACVGGTSVR--EDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDCIKMFVLDE 199

Query: 253 ADHMLGEVIIS--FFLFLLLKTLFQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEELSL 312
           AD ML        + +F LL    QV +FSAT          + +    ++ VK++EL+L
Sbjct: 200 ADEMLSRGFKDQIYDIFQLLPPKIQVGVFSATMPPEALEITRKFMSKPVRILVKRDELTL 259

Query: 313 ESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVGTRKSAGVLHKALVDLGYDVTT 372
           E +KQF +    E  K+  + D    LA  + Q++IFV TR+    L   +    + V+ 
Sbjct: 260 EGIKQFYVNVEKEDWKLETLCDLYETLA--ITQSVIFVNTRRKVDWLTDKMRSRDHTVSA 319

Query: 373 IQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQAMK 432
             G M  + RD I++EF+ G ++VLI+TDLLARG D QQV+LVIN+DLP           
Sbjct: 320 THGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINFDLP----------- 379

Query: 433 YRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVRDT 492
                 ++P  E YLHRIGR+GRFGRKG   N +  D +    D IQK +   V E+   
Sbjct: 380 ------TQP--ENYLHRIGRSGRFGRKGVAINFVTLDDQRMLFD-IQKFYNVVVEELPSN 411

BLAST of CSPI03G33840 vs. TAIR 10
Match: AT3G13920.3 (eukaryotic translation initiation factor 4A1 )

HSP 1 Score: 202.6 bits (514), Expect = 7.5e-52
Identity = 143/401 (35.66%), Postives = 207/401 (51.62%), Query Frame = 0

Query: 96  TFEDLNLSKELLKGLYVEMRFHKPSKIQAISLPMILTPPYKHL--IAQAHNGSGKTTCFV 155
           +F+ + L + LL+G+Y    F KPS IQ       + P  K L  I QA +G+GKT  F 
Sbjct: 30  SFDAMGLQENLLRGIYA-YGFEKPSAIQQRG----IVPFCKGLDVIQQAQSGTGKTATFC 89

Query: 156 LGMLSRVDVNLKAPQAFCICPTRELAMQNIEVLKKMGKYTGITSECAVPADSANYMPVSK 215
            G+L ++D +L   QA  + PTRELA Q  +V++ +G Y G+     V   S       +
Sbjct: 90  SGVLQQLDFSLIQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVR--EDQR 149

Query: 216 RPPTTAQVVIGTPGTIKKWMSGRKLGVSCVKILVFDEADHMLGEVIIS--FFLFLLLKTL 275
                  VV+GTPG +   +  + L    +K+ V DEAD ML        + +F LL   
Sbjct: 150 ILQAGVHVVVGTPGRVFDMLKRQSLRADNIKMFVLDEADEMLSRGFKDQIYDIFQLLPPK 209

Query: 276 FQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEELSLESVKQFKLICPNEQTKIRVIKDR 335
            QV +FSAT          + +    ++ VK++EL+LE +KQF +    E+ K+  + D 
Sbjct: 210 IQVGVFSATMPPEALEITRKFMSKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDL 269

Query: 336 IFELADKLGQTIIFVGTRKSAGVLHKALVDLGYDVTTIQGAMTNDIRDKIIKEFKDGLTK 395
              LA  + Q++IFV TR+    L   +    + V+   G M  + RD I++EF+ G ++
Sbjct: 270 YETLA--ITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSR 329

Query: 396 VLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQAMKYRSSSSSEPNFEVYLHRIGRAGR 455
           VLI+TDLLARG D QQV+LVIN+DLP                 ++P  E YLHRIGR+GR
Sbjct: 330 VLITTDLLARGIDVQQVSLVINFDLP-----------------TQP--ENYLHRIGRSGR 389

Query: 456 FGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVRDTDEDI 493
           FGRKG   N +  D E    D IQK +   V E+     D+
Sbjct: 390 FGRKGVAINFVTRDDERMLFD-IQKFYNVVVEELPSNVADL 401

BLAST of CSPI03G33840 vs. TAIR 10
Match: AT3G13920.1 (eukaryotic translation initiation factor 4A1 )

HSP 1 Score: 201.4 bits (511), Expect = 1.7e-51
Identity = 148/424 (34.91%), Postives = 215/424 (50.71%), Query Frame = 0

Query: 73  LEEPIDSNITAVTTGDTPYSSASTFEDLNLSKELLKGLYVEMRFHKPSKIQAISLPMILT 132
           L E ++      T+ D  + S   F+ + L + LL+G+Y    F KPS IQ       + 
Sbjct: 20  LNEVLEGQDEFFTSYDDVHES---FDAMGLQENLLRGIYA-YGFEKPSAIQQRG----IV 79

Query: 133 PPYKHL--IAQAHNGSGKTTCFVLGMLSRVDVNLKAPQAFCICPTRELAMQNIEVLKKMG 192
           P  K L  I QA +G+GKT  F  G+L ++D +L   QA  + PTRELA Q  +V++ +G
Sbjct: 80  PFCKGLDVIQQAQSGTGKTATFCSGVLQQLDFSLIQCQALVLAPTRELAQQIEKVMRALG 139

Query: 193 KYTGITSECAVPADSANYMPVSKRPPTTAQVVIGTPGTIKKWMSGRKLGVSCVKILVFDE 252
            Y G+     V   S       +       VV+GTPG +   +  + L    +K+ V DE
Sbjct: 140 DYLGVKVHACVGGTSVR--EDQRILQAGVHVVVGTPGRVFDMLKRQSLRADNIKMFVLDE 199

Query: 253 ADHMLGEVIIS--FFLFLLLKTLFQVLLFSATFDENVKNFVSRVVKDYNQLFVKKEELSL 312
           AD ML        + +F LL    QV +FSAT          + +    ++ VK++EL+L
Sbjct: 200 ADEMLSRGFKDQIYDIFQLLPPKIQVGVFSATMPPEALEITRKFMSKPVRILVKRDELTL 259

Query: 313 ESVKQFKLICPNEQTKIRVIKDRIFELADKLGQTIIFVGTRKSAGVLHKALVDLGYDVTT 372
           E +KQF +    E+ K+  + D    LA  + Q++IFV TR+    L   +    + V+ 
Sbjct: 260 EGIKQFYVNVEKEEWKLETLCDLYETLA--ITQSVIFVNTRRKVDWLTDKMRSRDHTVSA 319

Query: 373 IQGAMTNDIRDKIIKEFKDGLTKVLISTDLLARGFDQQQVNLVINYDLPLKHDPSSQAMK 432
             G M  + RD I++EF+ G ++VLI+TDLLARG D QQV+LVIN+DLP           
Sbjct: 320 THGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINFDLP----------- 379

Query: 433 YRSSSSSEPNFEVYLHRIGRAGRFGRKGAVFNLLCGDQEIKRMDKIQKHFGSEVIEVRDT 492
                 ++P  E YLHRIGR+GRFGRKG   N +  D E    D IQK +   V E+   
Sbjct: 380 ------TQP--ENYLHRIGRSGRFGRKGVAINFVTRDDERMLFD-IQKFYNVVVEELPSN 411

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93ZG76.8e-15157.48DEAD-box ATP-dependent RNA helicase 38 OS=Arabidopsis thaliana OX=3702 GN=RH38 P... [more]
Q10RI71.1e-13559.13DEAD-box ATP-dependent RNA helicase 38 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q4P7Z82.0e-7336.46ATP-dependent RNA helicase DBP5 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=2... [more]
Q6CJU17.5e-7337.50ATP-dependent RNA helicase DBP5 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS ... [more]
Q75C399.8e-7337.09ATP-dependent RNA helicase DBP5 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.... [more]
Match NameE-valueIdentityDescription
A0A0A0LDT38.7e-27495.87Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G746560 PE=4 SV=1[more]
A0A5A7TRB42.9e-26189.12DEAD-box ATP-dependent RNA helicase 38 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B6011.6e-25992.29DEAD-box ATP-dependent RNA helicase 38 OS=Cucumis melo OX=3656 GN=LOC103486545 P... [more]
A0A5D3DNK61.7e-25387.43DEAD-box ATP-dependent RNA helicase 38 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S4E4191.4e-23183.10DEAD-box ATP-dependent RNA helicase 38-like OS=Cucumis melo OX=3656 GN=LOC103501... [more]
Match NameE-valueIdentityDescription
XP_004137993.38.9e-27395.87DEAD-box ATP-dependent RNA helicase 38 [Cucumis sativus] >KAE8651061.1 hypotheti... [more]
KAA0043945.16.0e-26189.12DEAD-box ATP-dependent RNA helicase 38 [Cucumis melo var. makuwa][more]
XP_008442767.13.3e-25992.29PREDICTED: DEAD-box ATP-dependent RNA helicase 38 [Cucumis melo][more]
TYK25195.13.5e-25387.43DEAD-box ATP-dependent RNA helicase 38 [Cucumis melo var. makuwa][more]
XP_038906287.13.9e-23684.41DEAD-box ATP-dependent RNA helicase 38 [Benincasa hispida] >XP_038906288.1 DEAD-... [more]
Match NameE-valueIdentityDescription
AT3G53110.14.9e-15257.48P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G72730.14.0e-5335.16DEA(D/H)-box RNA helicase family protein [more]
AT1G54270.15.7e-5235.14eif4a-2 [more]
AT3G13920.37.5e-5235.66eukaryotic translation initiation factor 4A1 [more]
AT3G13920.11.7e-5134.91eukaryotic translation initiation factor 4A1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001650Helicase, C-terminalSMARTSM00490helicmild6coord: 353..453
e-value: 9.8E-24
score: 94.9
IPR001650Helicase, C-terminalPFAMPF00271Helicase_Ccoord: 325..453
e-value: 3.4E-23
score: 82.1
IPR001650Helicase, C-terminalPROSITEPS51194HELICASE_CTERcoord: 327..493
score: 19.715839
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 115..314
e-value: 1.7E-33
score: 127.2
IPR014001Helicase superfamily 1/2, ATP-binding domainPROSITEPS51192HELICASE_ATP_BIND_1coord: 129..299
score: 21.738379
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 306..493
e-value: 6.7E-42
score: 144.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 78..305
e-value: 5.2E-49
score: 168.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 167..469
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 121..287
e-value: 4.7E-29
score: 101.4
NoneNo IPR availablePANTHERPTHR47958ATP-DEPENDENT RNA HELICASE DBP3coord: 74..483
NoneNo IPR availablePANTHERPTHR47958:SF31DEAD-BOX HELICASE DBP80coord: 74..483
NoneNo IPR availableCDDcd18787SF2_C_DEADcoord: 311..462
e-value: 9.87246E-40
score: 138.409
NoneNo IPR availableCDDcd17963DEADc_DDX19_DDX25coord: 102..299
e-value: 1.85246E-88
score: 267.519
IPR014014RNA helicase, DEAD-box type, Q motifPROSITEPS51195Q_MOTIFcoord: 95..124
score: 9.577528

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G33840.1CSPI03G33840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016973 poly(A)+ mRNA export from nucleus
cellular_component GO:0010494 cytoplasmic stress granule
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003723 RNA binding
molecular_function GO:0003724 RNA helicase activity
molecular_function GO:0003676 nucleic acid binding