CSPI03G30360 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G30360
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionNon-specific serine/threonine protein kinase
LocationChr3: 27803902 .. 27805824 (+)
RNA-Seq ExpressionCSPI03G30360
SyntenyCSPI03G30360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAACGCCAAAATTAAACAAACTTAATCCCTCCCTCTTCTTCAATCTTCAATCTTCAATCTTCAATCCTCAATGTCTTGGCTGGATCTCATTGCTTCACTCTTTCAATTCTTCTCCTCTTTTCTTTTCTAACCATCATTTTCATTATCCAATCCCCCTGTCTCTTCCACTCTCCTCACTCATTCACTCACTTCCTATTTTGTTATACCAATGTTCTAACTCCTTCTTCCAAACTCTGTTTTTACCCTTTGTTGAACAGAAATCGGACATGGCGGGTGGCGCGGCCCAGGTTAGGAATGTTCTTTTTAGGAAATACGAAATGGGTCGGCTTTTGGGGAAAGGGACCTTTGCAAAGGTCTATTATGGAAGAAACTTGATGACCCACGAGAGTGTTGCCATTAAAGTGATTCTCAAGGACAGGGTGAAGAAAGAGGGGCTTCTTGAGCAAATCCGGCGAGAAATTTCTGTTATGGGCTTGGTTCGTCATCCTCATATTGTGGAATTGAAAGAAGTTATGGCGACCAAATCGAAGGTGTATTTTGTGATGGAGTATGTTAATGGAGGCGAACTGTTTGTGAAGTTGTCTCAACTAGGGAAATTGACTGAGGATGGAGCAAGGAATTATTTTCAACAGCTTATCTCTGCCGTGGACTTTTGCCATAGCCGTGGTGTCTCTCACCGTGATTTGAAGCCTGAGAATCTCCTTCTTGACGAGAATGGGAATTTGAAGGTTTCTGATTTTGGTCTCTCTGCCCTGCCGGAGCAGCTACGGATTGATGGTTTGCTTCACACACGCTGTGGAACTCCGGCTTATGTGGCGCCTGAGGTGCTCAGAAAGAGAGGATACGATGGCGCTAAGGCGGATATTTGGTCTTGCGGGGTCATTTTGTTTGTGTTGCTTGCTGGGACTTTGCCTTTTCGAGCTGATAACGCGATGAAGTTGTATAGGAAAATTTTCAAGGCGGAGTATGAGTTTCCTCCATGGTTTTCTTCAGAAGCCAAGGAAATGGTGTCTAAGCTTCTGGTTGTTGATCCTCAAAAGAGAGCTTCAATGCTTGAGATAATGCATAGTCCTTGGTTTCAAAAGGGCTTCACGAAGCCAGTTTCATTCATTGAGAAAGATGAATTCAACAATGAATTCTCTGAATTGGCTAATCCCAAGTTGTTGTCATCTTCTCCTCCTTTTTACAATGCATTTGAGTTCATTTCCTCAATGTCCTCAGGTTTTGACTTGTCTAGTTTGTTTGAAAGCAAGAAGAAACAAGGCTCTATTTTCACTTCAAAATGCCCTGCTTCAGCCATTTTAGCCAAGCTTCAATCTCTGGCCGTGAAGCTCAACTTCAAAGCTACTAACTTAAAGGAGTTCAAGGTGAGACTGCAGGCCAAGGAGGAGGGGAGGAAAGGGAAGTTGACAGTGACTGCAGAGGTTTACGAGGTGGCACCGGAGTTGGCAATCGTAGAGTTCTCGAAGTCGGCCGGCGACACTTTGGAATACAACAAGTTATGTGAGAAGGATGTGAGGCCCGCATTGAAAGACATAGTTTGGAGCTGGCAAGGAGAAAGTGAAGTGCCTTTAACAGTGGATTCTATGCAAAATGATCATTGAGGAAGAAAAAGAAGCCATTTTTGTGCATGGTGAACATAATTTTCACTTTGAAGTTCCCTTTTTTCATGTGTTTTTGGAGAAGAGAAGGATATATAGTACAGCATCGAATGGTTTTTGTTTAGATTAGAAGACAGTGGGAGTCGATAGCAATGCGTTTGGAATTCGCTAATTTTGTGGATACTTCATATAATTTGCTTGTAAAATATACTTTGCTTTGAGAGTGAAGGCCAGTTTATTAGTTTTGATAAATTTCATATAACAAATTTGTGTTGCATAGGCTTTTGACCATTTCTAGTCTGCCTTACGTTTTTGGGGGTC

mRNA sequence

CAAACGCCAAAATTAAACAAACTTAATCCCTCCCTCTTCTTCAATCTTCAATCTTCAATCTTCAATCCTCAATGTCTTGGCTGGATCTCATTGCTTCACTCTTTCAATTCTTCTCCTCTTTTCTTTTCTAACCATCATTTTCATTATCCAATCCCCCTGTCTCTTCCACTCTCCTCACTCATTCACTCACTTCCTATTTTGTTATACCAATGTTCTAACTCCTTCTTCCAAACTCTGTTTTTACCCTTTGTTGAACAGAAATCGGACATGGCGGGTGGCGCGGCCCAGGTTAGGAATGTTCTTTTTAGGAAATACGAAATGGGTCGGCTTTTGGGGAAAGGGACCTTTGCAAAGGTCTATTATGGAAGAAACTTGATGACCCACGAGAGTGTTGCCATTAAAGTGATTCTCAAGGACAGGGTGAAGAAAGAGGGGCTTCTTGAGCAAATCCGGCGAGAAATTTCTGTTATGGGCTTGGTTCGTCATCCTCATATTGTGGAATTGAAAGAAGTTATGGCGACCAAATCGAAGGTGTATTTTGTGATGGAGTATGTTAATGGAGGCGAACTGTTTGTGAAGTTGTCTCAACTAGGGAAATTGACTGAGGATGGAGCAAGGAATTATTTTCAACAGCTTATCTCTGCCGTGGACTTTTGCCATAGCCGTGGTGTCTCTCACCGTGATTTGAAGCCTGAGAATCTCCTTCTTGACGAGAATGGGAATTTGAAGGTTTCTGATTTTGGTCTCTCTGCCCTGCCGGAGCAGCTACGGATTGATGGTTTGCTTCACACACGCTGTGGAACTCCGGCTTATGTGGCGCCTGAGGTGCTCAGAAAGAGAGGATACGATGGCGCTAAGGCGGATATTTGGTCTTGCGGGGTCATTTTGTTTGTGTTGCTTGCTGGGACTTTGCCTTTTCGAGCTGATAACGCGATGAAGTTGTATAGGAAAATTTTCAAGGCGGAGTATGAGTTTCCTCCATGGTTTTCTTCAGAAGCCAAGGAAATGGTGTCTAAGCTTCTGGTTGTTGATCCTCAAAAGAGAGCTTCAATGCTTGAGATAATGCATAGTCCTTGGTTTCAAAAGGGCTTCACGAAGCCAGTTTCATTCATTGAGAAAGATGAATTCAACAATGAATTCTCTGAATTGGCTAATCCCAAGTTGTTGTCATCTTCTCCTCCTTTTTACAATGCATTTGAGTTCATTTCCTCAATGTCCTCAGGTTTTGACTTGTCTAGTTTGTTTGAAAGCAAGAAGAAACAAGGCTCTATTTTCACTTCAAAATGCCCTGCTTCAGCCATTTTAGCCAAGCTTCAATCTCTGGCCGTGAAGCTCAACTTCAAAGCTACTAACTTAAAGGAGTTCAAGGTGAGACTGCAGGCCAAGGAGGAGGGGAGGAAAGGGAAGTTGACAGTGACTGCAGAGGTTTACGAGGTGGCACCGGAGTTGGCAATCGTAGAGTTCTCGAAGTCGGCCGGCGACACTTTGGAATACAACAAGTTATGTGAGAAGGATGTGAGGCCCGCATTGAAAGACATAGTTTGGAGCTGGCAAGGAGAAAGTGAAGTGCCTTTAACAGTGGATTCTATGCAAAATGATCATTGAGGAAGAAAAAGAAGCCATTTTTGTGCATGGTGAACATAATTTTCACTTTGAAGTTCCCTTTTTTCATGTGTTTTTGGAGAAGAGAAGGATATATAGTACAGCATCGAATGGTTTTTGTTTAGATTAGAAGACAGTGGGAGTCGATAGCAATGCGTTTGGAATTCGCTAATTTTGTGGATACTTCATATAATTTGCTTGTAAAATATACTTTGCTTTGAGAGTGAAGGCCAGTTTATTAGTTTTGATAAATTTCATATAACAAATTTGTGTTGCATAGGCTTTTGACCATTTCTAGTCTGCCTTACGTTTTTGGGGGTC

Coding sequence (CDS)

ATGGCGGGTGGCGCGGCCCAGGTTAGGAATGTTCTTTTTAGGAAATACGAAATGGGTCGGCTTTTGGGGAAAGGGACCTTTGCAAAGGTCTATTATGGAAGAAACTTGATGACCCACGAGAGTGTTGCCATTAAAGTGATTCTCAAGGACAGGGTGAAGAAAGAGGGGCTTCTTGAGCAAATCCGGCGAGAAATTTCTGTTATGGGCTTGGTTCGTCATCCTCATATTGTGGAATTGAAAGAAGTTATGGCGACCAAATCGAAGGTGTATTTTGTGATGGAGTATGTTAATGGAGGCGAACTGTTTGTGAAGTTGTCTCAACTAGGGAAATTGACTGAGGATGGAGCAAGGAATTATTTTCAACAGCTTATCTCTGCCGTGGACTTTTGCCATAGCCGTGGTGTCTCTCACCGTGATTTGAAGCCTGAGAATCTCCTTCTTGACGAGAATGGGAATTTGAAGGTTTCTGATTTTGGTCTCTCTGCCCTGCCGGAGCAGCTACGGATTGATGGTTTGCTTCACACACGCTGTGGAACTCCGGCTTATGTGGCGCCTGAGGTGCTCAGAAAGAGAGGATACGATGGCGCTAAGGCGGATATTTGGTCTTGCGGGGTCATTTTGTTTGTGTTGCTTGCTGGGACTTTGCCTTTTCGAGCTGATAACGCGATGAAGTTGTATAGGAAAATTTTCAAGGCGGAGTATGAGTTTCCTCCATGGTTTTCTTCAGAAGCCAAGGAAATGGTGTCTAAGCTTCTGGTTGTTGATCCTCAAAAGAGAGCTTCAATGCTTGAGATAATGCATAGTCCTTGGTTTCAAAAGGGCTTCACGAAGCCAGTTTCATTCATTGAGAAAGATGAATTCAACAATGAATTCTCTGAATTGGCTAATCCCAAGTTGTTGTCATCTTCTCCTCCTTTTTACAATGCATTTGAGTTCATTTCCTCAATGTCCTCAGGTTTTGACTTGTCTAGTTTGTTTGAAAGCAAGAAGAAACAAGGCTCTATTTTCACTTCAAAATGCCCTGCTTCAGCCATTTTAGCCAAGCTTCAATCTCTGGCCGTGAAGCTCAACTTCAAAGCTACTAACTTAAAGGAGTTCAAGGTGAGACTGCAGGCCAAGGAGGAGGGGAGGAAAGGGAAGTTGACAGTGACTGCAGAGGTTTACGAGGTGGCACCGGAGTTGGCAATCGTAGAGTTCTCGAAGTCGGCCGGCGACACTTTGGAATACAACAAGTTATGTGAGAAGGATGTGAGGCCCGCATTGAAAGACATAGTTTGGAGCTGGCAAGGAGAAAGTGAAGTGCCTTTAACAGTGGATTCTATGCAAAATGATCATTGA

Protein sequence

MAGGAAQVRNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQIRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWFSSEAKEMVSKLLVVDPQKRASMLEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLLSSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKATNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPALKDIVWSWQGESEVPLTVDSMQNDH*
Homology
BLAST of CSPI03G30360 vs. ExPASy Swiss-Prot
Match: Q8W1D5 (CBL-interacting serine/threonine-protein kinase 25 OS=Arabidopsis thaliana OX=3702 GN=CIPK25 PE=2 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 1.0e-166
Identity = 293/434 (67.51%), Postives = 353/434 (81.34%), Query Frame = 0

Query: 7   QVRNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQIRREIS 66
           Q   VLF KYEMGRLLGKGTF KVYYG+ + T ESVAIK+I KD+VK+EG++EQI+REIS
Sbjct: 34  QQLRVLFAKYEMGRLLGKGTFGKVYYGKEITTGESVAIKIINKDQVKREGMMEQIKREIS 93

Query: 67  VMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISA 126
           +M LVRHP+IVELKEVMATK+K++F+MEYV GGELF K+ + GKL ED AR YFQQLISA
Sbjct: 94  IMRLVRHPNIVELKEVMATKTKIFFIMEYVKGGELFSKIVK-GKLKEDSARKYFQQLISA 153

Query: 127 VDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPE 186
           VDFCHSRGVSHRDLKPENLL+DENG+LKVSDFGLSALPEQ+  DGLLHT+CGTPAYVAPE
Sbjct: 154 VDFCHSRGVSHRDLKPENLLVDENGDLKVSDFGLSALPEQILQDGLLHTQCGTPAYVAPE 213

Query: 187 VLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWFSSEAKE 246
           VLRK+GYDGAK DIWSCG+IL+VLLAG LPF+ +N MK+YRKIFK+E+E+PPWFS E+K 
Sbjct: 214 VLRKKGYDGAKGDIWSCGIILYVLLAGFLPFQDENLMKMYRKIFKSEFEYPPWFSPESKR 273

Query: 247 MVSKLLVVDPQKRASMLEIMHSPWFQKGFTKPVSF-IEKDEFNNEFSE-------LANPK 306
           ++SKLLVVDP KR S+  IM +PWF+K    P+ F I++ E  N   E        A   
Sbjct: 274 LISKLLVVDPNKRISIPAIMRTPWFRKNINSPIEFKIDELEIQNVEDETPTTTATTATTT 333

Query: 307 LLSSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNF 366
               SP F+NAFEFISSMSSGFDLSSLFESK+K  S+FTS+  AS I+ KL+ +  ++N 
Sbjct: 334 TTPVSPKFFNAFEFISSMSSGFDLSSLFESKRKLRSMFTSRWSASEIMGKLEGIGKEMNM 393

Query: 367 KATNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVR 426
           K    K+FKV+L  K EGRKG++ VTAEV+EVAPE+A+VE  KSAGDTLEYN+L E+ VR
Sbjct: 394 KVKRTKDFKVKLFGKTEGRKGQIAVTAEVFEVAPEVAVVELCKSAGDTLEYNRLYEEHVR 453

Query: 427 PALKDIVWSWQGES 433
           PAL++IVWSW G++
Sbjct: 454 PALEEIVWSWHGDN 466

BLAST of CSPI03G30360 vs. ExPASy Swiss-Prot
Match: Q9LEU7 (CBL-interacting serine/threonine-protein kinase 5 OS=Arabidopsis thaliana OX=3702 GN=CIPK5 PE=1 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 3.7e-164
Identity = 286/434 (65.90%), Postives = 358/434 (82.49%), Query Frame = 0

Query: 9   RNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRV-KKEGLLEQIRREISV 68
           R VLF KYEMGRLLGKGTFAKVYYG+ ++  E VAIKVI KD+V K+ G++EQI+REIS+
Sbjct: 5   RRVLFGKYEMGRLLGKGTFAKVYYGKEIIGGECVAIKVINKDQVMKRPGMMEQIKREISI 64

Query: 69  MGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAV 128
           M LVRHP+IVELKEVMATK+K++FVME+V GGELF K+S+ GKL ED AR YFQQLISAV
Sbjct: 65  MKLVRHPNIVELKEVMATKTKIFFVMEFVKGGELFCKISK-GKLHEDAARRYFQQLISAV 124

Query: 129 DFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEV 188
           D+CHSRGVSHRDLKPENLLLDENG+LK+SDFGLSALPEQ+  DGLLHT+CGTPAYVAPEV
Sbjct: 125 DYCHSRGVSHRDLKPENLLLDENGDLKISDFGLSALPEQILQDGLLHTQCGTPAYVAPEV 184

Query: 189 LRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWFSSEAKEM 248
           L+K+GYDGAKADIWSCGV+L+VLLAG LPF+ +N M +YRKIF+A++EFPPWFS EA+ +
Sbjct: 185 LKKKGYDGAKADIWSCGVVLYVLLAGCLPFQDENLMNMYRKIFRADFEFPPWFSPEARRL 244

Query: 249 VSKLLVVDPQKRASMLEIMHSPWFQKGFTKPVSF------IEKDEFNNEFSEL---ANPK 308
           +SKLLVVDP +R S+  IM +PW +K FT P++F        +   NNE  E       +
Sbjct: 245 ISKLLVVDPDRRISIPAIMRTPWLRKNFTPPLAFKIDEPICSQSSKNNEEEEEDGDCENQ 304

Query: 309 LLSSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNF 368
               SP F+NAFEFISSMSSGFDLSSLFESK+K  S+FTS+  A+ ++ K++++  ++N 
Sbjct: 305 TEPISPKFFNAFEFISSMSSGFDLSSLFESKRKVQSVFTSRSSATEVMEKIETVTKEMNM 364

Query: 369 KATNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVR 428
           K    K+FKV+++ K EGRKG+L++TAEV+EVAPE+++VEF KSAGDTLEY++L E++VR
Sbjct: 365 KVKRTKDFKVKMEGKTEGRKGRLSMTAEVFEVAPEISVVEFCKSAGDTLEYDRLYEEEVR 424

Query: 429 PALKDIVWSWQGES 433
           PAL DIVWSW G++
Sbjct: 425 PALNDIVWSWHGDN 437

BLAST of CSPI03G30360 vs. ExPASy Swiss-Prot
Match: Q9SEZ7 (CBL-interacting serine/threonine-protein kinase 16 OS=Arabidopsis thaliana OX=3702 GN=CIPK16 PE=1 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.2e-146
Identity = 267/439 (60.82%), Postives = 335/439 (76.31%), Query Frame = 0

Query: 11  VLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRV-KKEGLLEQIRREISVMG 70
           VLF KY +GRLLG G FAKVY+G  + T + VAIKVI KD V K+ G++EQI REI+VM 
Sbjct: 10  VLFDKYNIGRLLGTGNFAKVYHGTEISTGDDVAIKVIKKDHVFKRRGMMEQIEREIAVMR 69

Query: 71  LVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAVDF 130
           L+RHP++VEL+EVMATK K++FVMEYVNGGELF  + + GKL ED AR YFQQLISAVDF
Sbjct: 70  LLRHPNVVELREVMATKKKIFFVMEYVNGGELFEMIDRDGKLPEDLARKYFQQLISAVDF 129

Query: 131 CHSRGVSHRDLKPENLLLDENGNLKVSDFGLSAL--PEQL-----RIDGLLHTRCGTPAY 190
           CHSRGV HRD+KPENLLLD  G+LKV+DFGLSAL  PE L       D LLHTRCGTPAY
Sbjct: 130 CHSRGVFHRDIKPENLLLDGEGDLKVTDFGLSALMMPEGLGGRRGSSDDLLHTRCGTPAY 189

Query: 191 VAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWFSS 250
           VAPEVLR +GYDGA ADIWSCG++L+ LLAG LPF  +N M LY KIFKAE EFPPWFS 
Sbjct: 190 VAPEVLRNKGYDGAMADIWSCGIVLYALLAGFLPFIDENVMTLYTKIFKAECEFPPWFSL 249

Query: 251 EAKEMVSKLLVVDPQKRASMLEIMHSPWFQKGFTKPVSFIEKDEF--------NNEFSEL 310
           E+KE++S+LLV DP++R SM EI   PWF+K FT  V+F   +            +  +L
Sbjct: 250 ESKELLSRLLVPDPEQRISMSEIKMIPWFRKNFTPSVAFSIDETIPSPPEPPTKKKKKDL 309

Query: 311 ANPKLLSSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAV 370
              +   +SP  +NAF+FI+SMSSGFDLS+LFE K+K   +FTSK PA ++  +L++ A 
Sbjct: 310 NEKEDDGASPRSFNAFQFITSMSSGFDLSNLFEIKRKPKRMFTSKFPAKSVKERLETAAR 369

Query: 371 KLNFKATNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCE 430
           +++ +  ++K+ K++LQ + EGRKG+L+VTAEV+EVAPE+++VEF K++GDTLEY   CE
Sbjct: 370 EMDMRVKHVKDCKMKLQRRTEGRKGRLSVTAEVFEVAPEVSVVEFCKTSGDTLEYYLFCE 429

Query: 431 KDVRPALKDIVWSWQGESE 434
            DVRPALKDIVWSWQG+ +
Sbjct: 430 DDVRPALKDIVWSWQGDDD 448

BLAST of CSPI03G30360 vs. ExPASy Swiss-Prot
Match: Q6ERS4 (CBL-interacting protein kinase 16 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK16 PE=2 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 3.8e-145
Identity = 251/426 (58.92%), Postives = 335/426 (78.64%), Query Frame = 0

Query: 9   RNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKK-EGLLEQIRREISV 68
           R ++  +YE+GRLLG+GTFAKVYYGR+L + ESVAIKVI K R+++ EG++EQ+RREIS+
Sbjct: 15  RKLVLGRYELGRLLGQGTFAKVYYGRDLRSGESVAIKVIDKARLRRTEGMVEQLRREISI 74

Query: 69  MGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAV 128
           M +VRHP++V ++EV+A++++V+ VMEY  GGELF K+++ G+LTE+ AR YFQQL++AV
Sbjct: 75  MRMVRHPNVVGIREVLASRARVFVVMEYARGGELFAKVAR-GRLTEEHARRYFQQLVAAV 134

Query: 129 DFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEV 188
            FCH RGV+HRDLKPENLLLDE G LKV+DFGL+ALPEQLR DGLLHT+CGTPAYVAPEV
Sbjct: 135 GFCHGRGVAHRDLKPENLLLDEEGRLKVTDFGLAALPEQLRQDGLLHTQCGTPAYVAPEV 194

Query: 189 LRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWFSSEAKEM 248
           LRKRGYDGA+AD+WSCGV+L+VLL G LPF+ +N  K+Y+KIFKAEY+ PPW S +A+ +
Sbjct: 195 LRKRGYDGARADLWSCGVVLYVLLCGFLPFQHENYAKMYQKIFKAEYQVPPWVSGDARRL 254

Query: 249 VSKLLVVDPQKRASMLEIMHSPWFQKGFTKPV--SFIEKDEFNNEFSELANPKLLSSSPP 308
           + +LLVVDP KR S+ EIM +PWF+KGF  PV  S +   ++  +   L      + SP 
Sbjct: 255 IVRLLVVDPAKRISIPEIMRTPWFKKGFVPPVPTSPVSPKKWEEDDVLLDGGDSGAMSPR 314

Query: 309 FYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKATNLKE 368
             NAF+ ISSMSSGFDLS +FES++K  ++FTS+ PA+ ++ KL+++   L + AT  K 
Sbjct: 315 TCNAFQLISSMSSGFDLSGMFESEQKAATVFTSRAPAATVIQKLEAVGRSLGYSATRGKG 374

Query: 369 FKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPALKDIV 428
           +K+RL+A  +G  G+L VT E  EVA ++A+VEF+  AGD LE+NK C  DVRP L DIV
Sbjct: 375 WKLRLEATADGANGRLAVTVEALEVAADVAVVEFAHDAGDELEFNKFCAVDVRPGLADIV 434

Query: 429 WSWQGE 432
           W+WQG+
Sbjct: 435 WAWQGD 439

BLAST of CSPI03G30360 vs. ExPASy Swiss-Prot
Match: Q9FJ54 (CBL-interacting serine/threonine-protein kinase 20 OS=Arabidopsis thaliana OX=3702 GN=CIPK20 PE=1 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 1.4e-134
Identity = 243/425 (57.18%), Postives = 313/425 (73.65%), Query Frame = 0

Query: 11  VLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQIRREISVMGL 70
           VL RKYE+GRLLG+GTFAKVY+ RN+ T ESVAIKVI K +V K GL++QI+REISVM L
Sbjct: 7   VLMRKYELGRLLGQGTFAKVYHARNIKTGESVAIKVIDKQKVAKVGLIDQIKREISVMRL 66

Query: 71  VRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAVDFC 130
           VRHPH+V L EVMA+K+K+YF MEYV GGELF K+S+ GKL E+ AR YFQQLI A+D+C
Sbjct: 67  VRHPHVVFLHEVMASKTKIYFAMEYVKGGELFDKVSK-GKLKENIARKYFQQLIGAIDYC 126

Query: 131 HSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRK 190
           HSRGV HRDLKPENLLLDENG+LK+SDFGLSAL E  + DGLLHT CGTPAYVAPEV+ K
Sbjct: 127 HSRGVYHRDLKPENLLLDENGDLKISDFGLSALRESKQQDGLLHTTCGTPAYVAPEVIGK 186

Query: 191 RGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWFSSEAKEMVSK 250
           +GYDGAKAD+WSCGV+L+VLLAG LPF   N +++YRKI K E++ P WF  E K+++S+
Sbjct: 187 KGYDGAKADVWSCGVVLYVLLAGFLPFHEQNLVEMYRKITKGEFKCPNWFPPEVKKLLSR 246

Query: 251 LLVVDPQKRASMLEIMHSPWFQKGFTK---PVSFIEKDEFNNEFSELANPKLLSSSPPFY 310
           +L  +P  R  + +IM + WFQKGF K   P S  E  + ++  S++      S  P  Y
Sbjct: 247 ILDPNPNSRIKIEKIMENSWFQKGFKKIETPKS-PESHQIDSLISDV--HAAFSVKPMSY 306

Query: 311 NAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKATNLKEFK 370
           NAF+ ISS+S GFDLS LFE +++  S FT+K  A  I++K + +A           +  
Sbjct: 307 NAFDLISSLSQGFDLSGLFEKEERSESKFTTKKDAKEIVSKFEEIATSSERFNLTKSDVG 366

Query: 371 VRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPALKDIVWS 430
           V+++ K EGRKG L +  E++EV     +VEF KS GDT+EY + C++++RP+LKDIVW 
Sbjct: 367 VKMEDKREGRKGHLAIDVEIFEVTNSFHMVEFKKSGGDTMEYKQFCDRELRPSLKDIVWK 426

Query: 431 WQGES 433
           WQG +
Sbjct: 427 WQGNN 427

BLAST of CSPI03G30360 vs. ExPASy TrEMBL
Match: A0A5A7T8B4 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold901G00290 PE=3 SV=1)

HSP 1 Score: 859.0 bits (2218), Expect = 8.9e-246
Identity = 429/445 (96.40%), Postives = 441/445 (99.10%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAG AAQVRNVLF KYEMGRLLGKGTFAKVYYGRN+MTHESVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGEAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNVMTHESVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQ GKLTEDGARNYF
Sbjct: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARNYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQLISAVDFCHSRGVSHRDLKPENLLLD+NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDQNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWF 240
           AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAE+EFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMLEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEAKEMVSKLLVVDPQKRASM+EIMHSPWFQ+GFTKPVSF+EKDEFNNEFSELANPKLL
Sbjct: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQEGFTKPVSFLEKDEFNNEFSELANPKLL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAI+AKLQSLAVKLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAIIAKLQSLAVKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPA 420
           TNL+EFKVRLQAKEEGRKGKLTVTAEV+EVAPELA+VEFSKSAGDTLEYNK CEKDVRPA
Sbjct: 361 TNLREFKVRLQAKEEGRKGKLTVTAEVHEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGESEVPLTVDSMQNDH 446
           LKDIVWSWQGE+EVPLTVDS+QNDH
Sbjct: 421 LKDIVWSWQGENEVPLTVDSLQNDH 445

BLAST of CSPI03G30360 vs. ExPASy TrEMBL
Match: A0A1S3CN52 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103502821 PE=3 SV=1)

HSP 1 Score: 859.0 bits (2218), Expect = 8.9e-246
Identity = 429/445 (96.40%), Postives = 441/445 (99.10%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAG AAQVRNVLF KYEMGRLLGKGTFAKVYYGRN+MTHESVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGEAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNVMTHESVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQ GKLTEDGARNYF
Sbjct: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARNYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQLISAVDFCHSRGVSHRDLKPENLLLD+NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDQNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWF 240
           AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAE+EFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMLEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEAKEMVSKLLVVDPQKRASM+EIMHSPWFQ+GFTKPVSF+EKDEFNNEFSELANPKLL
Sbjct: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQEGFTKPVSFLEKDEFNNEFSELANPKLL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAI+AKLQSLAVKLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAIIAKLQSLAVKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPA 420
           TNL+EFKVRLQAKEEGRKGKLTVTAEV+EVAPELA+VEFSKSAGDTLEYNK CEKDVRPA
Sbjct: 361 TNLREFKVRLQAKEEGRKGKLTVTAEVHEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGESEVPLTVDSMQNDH 446
           LKDIVWSWQGE+EVPLTVDS+QNDH
Sbjct: 421 LKDIVWSWQGENEVPLTVDSLQNDH 445

BLAST of CSPI03G30360 vs. ExPASy TrEMBL
Match: A0A6J1J0B3 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111481539 PE=3 SV=1)

HSP 1 Score: 778.9 bits (2010), Expect = 1.2e-221
Identity = 390/433 (90.07%), Postives = 410/433 (94.69%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAG AA  RNVLF KYEMGRLLGKGTFAKVY+GRNLMTH+SVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGRNLMTHDSVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           +RREISVMGLVRHPHIVELKEVMATKSKVYFVME VNGGELFVKLSQ GKLTEDG R YF
Sbjct: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMECVNGGELFVKLSQQGKLTEDGGRKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQL+SAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWF 240
           AYVAPEVLRKRGY+GAK+D+WSCGVILFVLLAG+LPF+ADN MKLYRKIFKAE+EFPPWF
Sbjct: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMLEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEAKEMVSKLLVVDP+KRASM EIM S WFQKGFTKP+SF +KDEF +E SELANPK+L
Sbjct: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSSWFQKGFTKPLSFNDKDEFKSEISELANPKML 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDL+SLFESKKKQ SIFTSKC ASAI+AKLQSLA KLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPA 420
           TN +EFKVRLQA EEGRKGKL VT EVYEVAPELA+VEFSKSAGDTLEYNK CEKDVRPA
Sbjct: 361 TNQREFKVRLQAMEEGRKGKLAVTMEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGESE 434
           LKDIVWSWQGE+E
Sbjct: 421 LKDIVWSWQGENE 433

BLAST of CSPI03G30360 vs. ExPASy TrEMBL
Match: A0A6J1F2J4 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111441609 PE=3 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 3.4e-221
Identity = 389/433 (89.84%), Postives = 409/433 (94.46%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAG AA  RNVLF KYEMGRLLGKGTFAKVY+G NLMTH+SVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           +RREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQ GKLTEDG R YF
Sbjct: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQL+SAVDFCHSRGVSHRDLKPENLLLD NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWF 240
           AYVAPEVLRKRGY+GAK+D+WSCGVILFVLLAG+LPF+ADN MKLYRKIFKAE+EFPPWF
Sbjct: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMLEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEAKEMVSKLLVVDP+KRASM EIM S WFQKGFTKP+SF +KDE N+E S+L NPKLL
Sbjct: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSCWFQKGFTKPLSFNDKDELNSEISDLTNPKLL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDL+SLFESKKKQ SIFTSKC ASAI+AKLQSLA KLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPA 420
           TN +EFKVRLQAKEEGRKGKL VT EVYEVAPELA+VEFSKSAGDTLEYNK CEKDVRPA
Sbjct: 361 TNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGESE 434
           LKDIVWSWQGE+E
Sbjct: 421 LKDIVWSWQGENE 433

BLAST of CSPI03G30360 vs. ExPASy TrEMBL
Match: A0A6J1DJA1 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111020996 PE=3 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 1.7e-217
Identity = 382/433 (88.22%), Postives = 406/433 (93.76%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAG AA  RNVLF KYEMGRLLGKGTFAKVYYGRNL+THESVAIKVILK+RVKKEGLL+Q
Sbjct: 1   MAGEAAPSRNVLFGKYEMGRLLGKGTFAKVYYGRNLVTHESVAIKVILKERVKKEGLLDQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           I+REISVMGLVRHPHIVELKEVMATKSKVYFVMEYV+GGELFVKLS+ GKLTED AR YF
Sbjct: 61  IQREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVSGGELFVKLSKQGKLTEDAARKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           +QL+SAVDFCHSRGVSHRDLKPENLL+DENGNLKVSDFGLSALPEQLR+DGLLHTRCGTP
Sbjct: 121 RQLVSAVDFCHSRGVSHRDLKPENLLVDENGNLKVSDFGLSALPEQLRVDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWF 240
           AYVAPEVLRKRGYDGAK DIWSCGVIL+VLLAG LPFRADN MKLYRKIFKA++EFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKVDIWSCGVILYVLLAGALPFRADNVMKLYRKIFKADFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMLEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSE+ EM+SK LVVDP+ R SM EIM SPWF+ GF+KP+SF EKDEFNNE SEL NPKL 
Sbjct: 241 SSESMEMISKFLVVDPENRVSMPEIMQSPWFRNGFSKPISFSEKDEFNNEISELGNPKL- 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKA 360
            SSPPFYNAFEFIS+MSSGFDLSSLFESKKK GSIFTSKC ASAI+AKLQSLA K+NFKA
Sbjct: 301 -SSPPFYNAFEFISTMSSGFDLSSLFESKKKSGSIFTSKCSASAIVAKLQSLAQKVNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPA 420
           TNLKE KVRLQAKEEGRKGKLTVTAEV+EVAPELA+VEFSKSAGDTLEYNK CEKDVRPA
Sbjct: 361 TNLKECKVRLQAKEEGRKGKLTVTAEVFEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGESE 434
           LKDIVWSWQGESE
Sbjct: 421 LKDIVWSWQGESE 431

BLAST of CSPI03G30360 vs. NCBI nr
Match: XP_004137659.1 (CBL-interacting serine/threonine-protein kinase 25 [Cucumis sativus])

HSP 1 Score: 881.3 bits (2276), Expect = 3.4e-252
Identity = 444/445 (99.78%), Postives = 445/445 (100.00%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAGGAAQVRNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGGAAQVRNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF
Sbjct: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWF 240
           AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMLEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEAKEMVSKLLVVDPQKRASM+EIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL
Sbjct: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPA 420
           TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPA
Sbjct: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPA 420

Query: 421 LKDIVWSWQGESEVPLTVDSMQNDH 446
           LKDIVWSWQGESEVPLTVDSMQNDH
Sbjct: 421 LKDIVWSWQGESEVPLTVDSMQNDH 445

BLAST of CSPI03G30360 vs. NCBI nr
Match: XP_008465153.1 (PREDICTED: CBL-interacting serine/threonine-protein kinase 25-like [Cucumis melo] >KAA0037705.1 CBL-interacting serine/threonine-protein kinase 25-like [Cucumis melo var. makuwa] >TYK27019.1 CBL-interacting serine/threonine-protein kinase 25-like [Cucumis melo var. makuwa])

HSP 1 Score: 859.0 bits (2218), Expect = 1.8e-245
Identity = 429/445 (96.40%), Postives = 441/445 (99.10%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAG AAQVRNVLF KYEMGRLLGKGTFAKVYYGRN+MTHESVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGEAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNVMTHESVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQ GKLTEDGARNYF
Sbjct: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARNYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQLISAVDFCHSRGVSHRDLKPENLLLD+NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDQNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWF 240
           AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAE+EFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMLEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEAKEMVSKLLVVDPQKRASM+EIMHSPWFQ+GFTKPVSF+EKDEFNNEFSELANPKLL
Sbjct: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQEGFTKPVSFLEKDEFNNEFSELANPKLL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAI+AKLQSLAVKLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAIIAKLQSLAVKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPA 420
           TNL+EFKVRLQAKEEGRKGKLTVTAEV+EVAPELA+VEFSKSAGDTLEYNK CEKDVRPA
Sbjct: 361 TNLREFKVRLQAKEEGRKGKLTVTAEVHEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGESEVPLTVDSMQNDH 446
           LKDIVWSWQGE+EVPLTVDS+QNDH
Sbjct: 421 LKDIVWSWQGENEVPLTVDSLQNDH 445

BLAST of CSPI03G30360 vs. NCBI nr
Match: XP_038905015.1 (CBL-interacting serine/threonine-protein kinase 25-like [Benincasa hispida])

HSP 1 Score: 817.4 bits (2110), Expect = 6.1e-233
Identity = 413/445 (92.81%), Postives = 424/445 (95.28%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAG A    NVLF KYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGEAPPRMNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQ GKLTEDGAR YF
Sbjct: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWF 240
           AYVAPEVLRK+GYDGAKADIWSCGVILFVLLAGTLPFRADN MKLYRKIFKAE+EFPPWF
Sbjct: 181 AYVAPEVLRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMLEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEA EMVSKLLVVDPQKRASM+EIM SPWFQKGFTKPVSF EKDEF+NEFSELANPK L
Sbjct: 241 SSEAMEMVSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSFAEKDEFSNEFSELANPKFL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKA 360
            SSPPFYNAFEFISSMSSGFDLSSLFESKK++GSIFTSKC  SAI+AKLQSLAVKLNFKA
Sbjct: 301 -SSPPFYNAFEFISSMSSGFDLSSLFESKKRRGSIFTSKCSPSAIIAKLQSLAVKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPA 420
           TNL+EFKVRLQAKEEGRKGKL VTAEVYEVAPELA+VEFSKS GDTLEYNK CEKDVRPA
Sbjct: 361 TNLREFKVRLQAKEEGRKGKLAVTAEVYEVAPELAVVEFSKSTGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGESEVPLTVDSMQNDH 446
           LKDIVWSWQGE+E  LTVDS+QNDH
Sbjct: 421 LKDIVWSWQGENECLLTVDSLQNDH 444

BLAST of CSPI03G30360 vs. NCBI nr
Match: KAG6581626.1 (CBL-interacting serine/threonine-protein kinase 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 783.1 bits (2021), Expect = 1.3e-222
Identity = 391/433 (90.30%), Postives = 411/433 (94.92%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAG AA  RNVLF KYEMGRLLGKGTFAKVY+GRNLMTH+SVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGRNLMTHDSVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           +RREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQ GKLTEDG R YF
Sbjct: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQL+SAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWF 240
           AYVAPEVLRKRGY+GAK+D+WSCGVILFVLLAG+LPF+ADN MKLYRKIFKAE+EFPPWF
Sbjct: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMLEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEAKEMVSKLLVVDP+KRASM EIM S WFQKGFTKP+SF +KDE N+E S+L NPKLL
Sbjct: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSSWFQKGFTKPLSFNDKDELNSEISDLTNPKLL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDL+SLFESKKKQ SIFTSKC ASAI+AKLQSLA KLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPA 420
           TN +EFKVRLQAKEEGRKGKL VT EVYEVAPELA+VEFSKSAGDTLEYNK CEKDVRPA
Sbjct: 361 TNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGESE 434
           LKDIVWSWQGE+E
Sbjct: 421 LKDIVWSWQGENE 433

BLAST of CSPI03G30360 vs. NCBI nr
Match: XP_023529102.1 (CBL-interacting serine/threonine-protein kinase 25-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 779.6 bits (2012), Expect = 1.4e-221
Identity = 388/433 (89.61%), Postives = 413/433 (95.38%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAG AA  R+VLF KYEMGRLLGKGTFAKVY+GRNL+TH++VAIKVILKDRVKK+GLL+Q
Sbjct: 1   MAGKAAPARHVLFGKYEMGRLLGKGTFAKVYHGRNLITHDTVAIKVILKDRVKKQGLLDQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           +RREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQ GKLTEDG R YF
Sbjct: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQL+SAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWF 240
           AYVAPEVLRKRGY+GAK+D+WSCGVILFVLLAG+LPF+ADN MKLYRKIFKAE+EFPPWF
Sbjct: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMLEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEAKEMVSKLLVVDP+KRASM EIM S WFQKGFTKP+SF +KDEFN+E SELANPKLL
Sbjct: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSSWFQKGFTKPLSFNDKDEFNSEISELANPKLL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKA 360
           SSSPPFYNAFEFIS+MSSGFDL+SLFESKKKQ SIFTSKC ASAI+AKLQSLA KLNFKA
Sbjct: 301 SSSPPFYNAFEFISTMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPA 420
           TN +EFKVRLQAKEEGRKGKL VT EVYEVAPELA+VEFSKSAGDTLEYNK CEKDVRPA
Sbjct: 361 TNQREFKVRLQAKEEGRKGKLAVTMEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGESE 434
           LKDIVWSWQGE+E
Sbjct: 421 LKDIVWSWQGENE 433

BLAST of CSPI03G30360 vs. TAIR 10
Match: AT5G25110.1 (CBL-interacting protein kinase 25 )

HSP 1 Score: 587.8 bits (1514), Expect = 7.3e-168
Identity = 293/434 (67.51%), Postives = 353/434 (81.34%), Query Frame = 0

Query: 7   QVRNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQIRREIS 66
           Q   VLF KYEMGRLLGKGTF KVYYG+ + T ESVAIK+I KD+VK+EG++EQI+REIS
Sbjct: 34  QQLRVLFAKYEMGRLLGKGTFGKVYYGKEITTGESVAIKIINKDQVKREGMMEQIKREIS 93

Query: 67  VMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISA 126
           +M LVRHP+IVELKEVMATK+K++F+MEYV GGELF K+ + GKL ED AR YFQQLISA
Sbjct: 94  IMRLVRHPNIVELKEVMATKTKIFFIMEYVKGGELFSKIVK-GKLKEDSARKYFQQLISA 153

Query: 127 VDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPE 186
           VDFCHSRGVSHRDLKPENLL+DENG+LKVSDFGLSALPEQ+  DGLLHT+CGTPAYVAPE
Sbjct: 154 VDFCHSRGVSHRDLKPENLLVDENGDLKVSDFGLSALPEQILQDGLLHTQCGTPAYVAPE 213

Query: 187 VLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWFSSEAKE 246
           VLRK+GYDGAK DIWSCG+IL+VLLAG LPF+ +N MK+YRKIFK+E+E+PPWFS E+K 
Sbjct: 214 VLRKKGYDGAKGDIWSCGIILYVLLAGFLPFQDENLMKMYRKIFKSEFEYPPWFSPESKR 273

Query: 247 MVSKLLVVDPQKRASMLEIMHSPWFQKGFTKPVSF-IEKDEFNNEFSE-------LANPK 306
           ++SKLLVVDP KR S+  IM +PWF+K    P+ F I++ E  N   E        A   
Sbjct: 274 LISKLLVVDPNKRISIPAIMRTPWFRKNINSPIEFKIDELEIQNVEDETPTTTATTATTT 333

Query: 307 LLSSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNF 366
               SP F+NAFEFISSMSSGFDLSSLFESK+K  S+FTS+  AS I+ KL+ +  ++N 
Sbjct: 334 TTPVSPKFFNAFEFISSMSSGFDLSSLFESKRKLRSMFTSRWSASEIMGKLEGIGKEMNM 393

Query: 367 KATNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVR 426
           K    K+FKV+L  K EGRKG++ VTAEV+EVAPE+A+VE  KSAGDTLEYN+L E+ VR
Sbjct: 394 KVKRTKDFKVKLFGKTEGRKGQIAVTAEVFEVAPEVAVVELCKSAGDTLEYNRLYEEHVR 453

Query: 427 PALKDIVWSWQGES 433
           PAL++IVWSW G++
Sbjct: 454 PALEEIVWSWHGDN 466

BLAST of CSPI03G30360 vs. TAIR 10
Match: AT5G10930.1 (CBL-interacting protein kinase 5 )

HSP 1 Score: 579.3 bits (1492), Expect = 2.6e-165
Identity = 286/434 (65.90%), Postives = 358/434 (82.49%), Query Frame = 0

Query: 9   RNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRV-KKEGLLEQIRREISV 68
           R VLF KYEMGRLLGKGTFAKVYYG+ ++  E VAIKVI KD+V K+ G++EQI+REIS+
Sbjct: 5   RRVLFGKYEMGRLLGKGTFAKVYYGKEIIGGECVAIKVINKDQVMKRPGMMEQIKREISI 64

Query: 69  MGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAV 128
           M LVRHP+IVELKEVMATK+K++FVME+V GGELF K+S+ GKL ED AR YFQQLISAV
Sbjct: 65  MKLVRHPNIVELKEVMATKTKIFFVMEFVKGGELFCKISK-GKLHEDAARRYFQQLISAV 124

Query: 129 DFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEV 188
           D+CHSRGVSHRDLKPENLLLDENG+LK+SDFGLSALPEQ+  DGLLHT+CGTPAYVAPEV
Sbjct: 125 DYCHSRGVSHRDLKPENLLLDENGDLKISDFGLSALPEQILQDGLLHTQCGTPAYVAPEV 184

Query: 189 LRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWFSSEAKEM 248
           L+K+GYDGAKADIWSCGV+L+VLLAG LPF+ +N M +YRKIF+A++EFPPWFS EA+ +
Sbjct: 185 LKKKGYDGAKADIWSCGVVLYVLLAGCLPFQDENLMNMYRKIFRADFEFPPWFSPEARRL 244

Query: 249 VSKLLVVDPQKRASMLEIMHSPWFQKGFTKPVSF------IEKDEFNNEFSEL---ANPK 308
           +SKLLVVDP +R S+  IM +PW +K FT P++F        +   NNE  E       +
Sbjct: 245 ISKLLVVDPDRRISIPAIMRTPWLRKNFTPPLAFKIDEPICSQSSKNNEEEEEDGDCENQ 304

Query: 309 LLSSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNF 368
               SP F+NAFEFISSMSSGFDLSSLFESK+K  S+FTS+  A+ ++ K++++  ++N 
Sbjct: 305 TEPISPKFFNAFEFISSMSSGFDLSSLFESKRKVQSVFTSRSSATEVMEKIETVTKEMNM 364

Query: 369 KATNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVR 428
           K    K+FKV+++ K EGRKG+L++TAEV+EVAPE+++VEF KSAGDTLEY++L E++VR
Sbjct: 365 KVKRTKDFKVKMEGKTEGRKGRLSMTAEVFEVAPEISVVEFCKSAGDTLEYDRLYEEEVR 424

Query: 429 PALKDIVWSWQGES 433
           PAL DIVWSW G++
Sbjct: 425 PALNDIVWSWHGDN 437

BLAST of CSPI03G30360 vs. TAIR 10
Match: AT2G25090.1 (CBL-interacting protein kinase 16 )

HSP 1 Score: 521.2 bits (1341), Expect = 8.4e-148
Identity = 267/439 (60.82%), Postives = 335/439 (76.31%), Query Frame = 0

Query: 11  VLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRV-KKEGLLEQIRREISVMG 70
           VLF KY +GRLLG G FAKVY+G  + T + VAIKVI KD V K+ G++EQI REI+VM 
Sbjct: 10  VLFDKYNIGRLLGTGNFAKVYHGTEISTGDDVAIKVIKKDHVFKRRGMMEQIEREIAVMR 69

Query: 71  LVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAVDF 130
           L+RHP++VEL+EVMATK K++FVMEYVNGGELF  + + GKL ED AR YFQQLISAVDF
Sbjct: 70  LLRHPNVVELREVMATKKKIFFVMEYVNGGELFEMIDRDGKLPEDLARKYFQQLISAVDF 129

Query: 131 CHSRGVSHRDLKPENLLLDENGNLKVSDFGLSAL--PEQL-----RIDGLLHTRCGTPAY 190
           CHSRGV HRD+KPENLLLD  G+LKV+DFGLSAL  PE L       D LLHTRCGTPAY
Sbjct: 130 CHSRGVFHRDIKPENLLLDGEGDLKVTDFGLSALMMPEGLGGRRGSSDDLLHTRCGTPAY 189

Query: 191 VAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWFSS 250
           VAPEVLR +GYDGA ADIWSCG++L+ LLAG LPF  +N M LY KIFKAE EFPPWFS 
Sbjct: 190 VAPEVLRNKGYDGAMADIWSCGIVLYALLAGFLPFIDENVMTLYTKIFKAECEFPPWFSL 249

Query: 251 EAKEMVSKLLVVDPQKRASMLEIMHSPWFQKGFTKPVSFIEKDEF--------NNEFSEL 310
           E+KE++S+LLV DP++R SM EI   PWF+K FT  V+F   +            +  +L
Sbjct: 250 ESKELLSRLLVPDPEQRISMSEIKMIPWFRKNFTPSVAFSIDETIPSPPEPPTKKKKKDL 309

Query: 311 ANPKLLSSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAV 370
              +   +SP  +NAF+FI+SMSSGFDLS+LFE K+K   +FTSK PA ++  +L++ A 
Sbjct: 310 NEKEDDGASPRSFNAFQFITSMSSGFDLSNLFEIKRKPKRMFTSKFPAKSVKERLETAAR 369

Query: 371 KLNFKATNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCE 430
           +++ +  ++K+ K++LQ + EGRKG+L+VTAEV+EVAPE+++VEF K++GDTLEY   CE
Sbjct: 370 EMDMRVKHVKDCKMKLQRRTEGRKGRLSVTAEVFEVAPEVSVVEFCKTSGDTLEYYLFCE 429

Query: 431 KDVRPALKDIVWSWQGESE 434
            DVRPALKDIVWSWQG+ +
Sbjct: 430 DDVRPALKDIVWSWQGDDD 448

BLAST of CSPI03G30360 vs. TAIR 10
Match: AT5G45820.1 (CBL-interacting protein kinase 20 )

HSP 1 Score: 481.1 bits (1237), Expect = 9.7e-136
Identity = 243/425 (57.18%), Postives = 313/425 (73.65%), Query Frame = 0

Query: 11  VLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQIRREISVMGL 70
           VL RKYE+GRLLG+GTFAKVY+ RN+ T ESVAIKVI K +V K GL++QI+REISVM L
Sbjct: 7   VLMRKYELGRLLGQGTFAKVYHARNIKTGESVAIKVIDKQKVAKVGLIDQIKREISVMRL 66

Query: 71  VRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAVDFC 130
           VRHPH+V L EVMA+K+K+YF MEYV GGELF K+S+ GKL E+ AR YFQQLI A+D+C
Sbjct: 67  VRHPHVVFLHEVMASKTKIYFAMEYVKGGELFDKVSK-GKLKENIARKYFQQLIGAIDYC 126

Query: 131 HSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRK 190
           HSRGV HRDLKPENLLLDENG+LK+SDFGLSAL E  + DGLLHT CGTPAYVAPEV+ K
Sbjct: 127 HSRGVYHRDLKPENLLLDENGDLKISDFGLSALRESKQQDGLLHTTCGTPAYVAPEVIGK 186

Query: 191 RGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWFSSEAKEMVSK 250
           +GYDGAKAD+WSCGV+L+VLLAG LPF   N +++YRKI K E++ P WF  E K+++S+
Sbjct: 187 KGYDGAKADVWSCGVVLYVLLAGFLPFHEQNLVEMYRKITKGEFKCPNWFPPEVKKLLSR 246

Query: 251 LLVVDPQKRASMLEIMHSPWFQKGFTK---PVSFIEKDEFNNEFSELANPKLLSSSPPFY 310
           +L  +P  R  + +IM + WFQKGF K   P S  E  + ++  S++      S  P  Y
Sbjct: 247 ILDPNPNSRIKIEKIMENSWFQKGFKKIETPKS-PESHQIDSLISDV--HAAFSVKPMSY 306

Query: 311 NAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKATNLKEFK 370
           NAF+ ISS+S GFDLS LFE +++  S FT+K  A  I++K + +A           +  
Sbjct: 307 NAFDLISSLSQGFDLSGLFEKEERSESKFTTKKDAKEIVSKFEEIATSSERFNLTKSDVG 366

Query: 371 VRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPALKDIVWS 430
           V+++ K EGRKG L +  E++EV     +VEF KS GDT+EY + C++++RP+LKDIVW 
Sbjct: 367 VKMEDKREGRKGHLAIDVEIFEVTNSFHMVEFKKSGGDTMEYKQFCDRELRPSLKDIVWK 426

Query: 431 WQGES 433
           WQG +
Sbjct: 427 WQGNN 427

BLAST of CSPI03G30360 vs. TAIR 10
Match: AT4G30960.1 (SOS3-interacting protein 3 )

HSP 1 Score: 452.2 bits (1162), Expect = 4.8e-127
Identity = 238/425 (56.00%), Postives = 309/425 (72.71%), Query Frame = 0

Query: 11  VLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQIRREISVMGL 70
           +L  +YE+GRLLG GTFAKVY+ RN+ T +SVA+KV+ K++V K G+++QI+REISVM +
Sbjct: 19  LLHGRYELGRLLGHGTFAKVYHARNIQTGKSVAMKVVGKEKVVKVGMVDQIKREISVMRM 78

Query: 71  VRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAVDFC 130
           V+HP+IVEL EVMA+KSK+YF ME V GGELF K+++ G+L ED AR YFQQLISAVDFC
Sbjct: 79  VKHPNIVELHEVMASKSKIYFAMELVRGGELFAKVAK-GRLREDVARVYFQQLISAVDFC 138

Query: 131 HSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRK 190
           HSRGV HRDLKPENLLLDE GNLKV+DFGLSA  E L+ DGLLHT CGTPAYVAPEV+ K
Sbjct: 139 HSRGVYHRDLKPENLLLDEEGNLKVTDFGLSAFTEHLKQDGLLHTTCGTPAYVAPEVILK 198

Query: 191 RGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWFSSEAKEMVSK 250
           +GYDGAKAD+WSCGVILFVLLAG LPF+ DN + +YRKI++ +++ P W SS+A+ +V+K
Sbjct: 199 KGYDGAKADLWSCGVILFVLLAGYLPFQDDNLVNMYRKIYRGDFKCPGWLSSDARRLVTK 258

Query: 251 LLVVDPQKRASMLEIMHSPWFQKGFTK----PV-SFIEKDEFNNEFSELANPKLLSSSPP 310
           LL  +P  R ++ ++M SPWF+K  T+    PV + I   E + +F    + +   +   
Sbjct: 259 LLDPNPNTRITIEKVMDSPWFKKQATRSRNEPVAATITTTEEDVDFLVHKSKEETET--- 318

Query: 311 FYNAFEFISSMSSGFDLSSLFESKKKQGS---IFTSKCPASAILAKLQSLAVKLNFKATN 370
             NAF  I ++S GFDLS LFE KKK+      F +  PAS++++ L+  A   N     
Sbjct: 319 -LNAFHII-ALSEGFDLSPLFEEKKKEEKREMRFATSRPASSVISSLEEAARVGNKFDVR 378

Query: 371 LKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPALK 428
             E +VR++ K+ GRKGKL V AE++ VAP   +VE  K  GDTLEYN  C   +RPALK
Sbjct: 379 KSESRVRIEGKQNGRKGKLAVEAEIFAVAPSFVVVEVKKDHGDTLEYNNFCSTALRPALK 437

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8W1D51.0e-16667.51CBL-interacting serine/threonine-protein kinase 25 OS=Arabidopsis thaliana OX=37... [more]
Q9LEU73.7e-16465.90CBL-interacting serine/threonine-protein kinase 5 OS=Arabidopsis thaliana OX=370... [more]
Q9SEZ71.2e-14660.82CBL-interacting serine/threonine-protein kinase 16 OS=Arabidopsis thaliana OX=37... [more]
Q6ERS43.8e-14558.92CBL-interacting protein kinase 16 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
Q9FJ541.4e-13457.18CBL-interacting serine/threonine-protein kinase 20 OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
A0A5A7T8B48.9e-24696.40Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3CN528.9e-24696.40Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10350... [more]
A0A6J1J0B31.2e-22190.07Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1F2J43.4e-22189.84Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1DJA11.7e-21788.22Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
XP_004137659.13.4e-25299.78CBL-interacting serine/threonine-protein kinase 25 [Cucumis sativus][more]
XP_008465153.11.8e-24596.40PREDICTED: CBL-interacting serine/threonine-protein kinase 25-like [Cucumis melo... [more]
XP_038905015.16.1e-23392.81CBL-interacting serine/threonine-protein kinase 25-like [Benincasa hispida][more]
KAG6581626.11.3e-22290.30CBL-interacting serine/threonine-protein kinase 25, partial [Cucurbita argyrospe... [more]
XP_023529102.11.4e-22189.61CBL-interacting serine/threonine-protein kinase 25-like [Cucurbita pepo subsp. p... [more]
Match NameE-valueIdentityDescription
AT5G25110.17.3e-16867.51CBL-interacting protein kinase 25 [more]
AT5G10930.12.6e-16565.90CBL-interacting protein kinase 5 [more]
AT2G25090.18.4e-14860.82CBL-interacting protein kinase 16 [more]
AT5G45820.19.7e-13657.18CBL-interacting protein kinase 20 [more]
AT4G30960.14.8e-12756.00SOS3-interacting protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 16..271
e-value: 2.7E-104
score: 362.5
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 16..271
e-value: 6.1E-76
score: 255.3
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 16..271
score: 52.15942
NoneNo IPR availableGENE3D3.30.310.80Kinase associated domain 1, KA1coord: 307..427
e-value: 3.0E-40
score: 139.2
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 7..306
e-value: 4.7E-104
score: 350.2
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 15..268
e-value: 8.6E-12
score: 42.5
NoneNo IPR availablePIRSRPIRSR037568-2PIRSR037568-2coord: 15..255
e-value: 8.0E-38
score: 127.3
NoneNo IPR availablePIRSRPIRSR037014-2PIRSR037014-2coord: 6..275
e-value: 2.3E-75
score: 252.3
NoneNo IPR availablePANTHERPTHR43895:SF119CBL-INTERACTING SERINE/THREONINE-PROTEIN KINASE 25-RELATEDcoord: 9..434
NoneNo IPR availablePANTHERPTHR43895FAMILY NOT NAMEDcoord: 9..434
NoneNo IPR availableCDDcd12195CIPK_Ccoord: 308..422
e-value: 3.9983E-44
score: 148.489
NoneNo IPR availableCDDcd14663STKc_SnRK3coord: 15..270
e-value: 1.97985E-167
score: 468.808
IPR004041NAF domainPFAMPF03822NAFcoord: 304..360
e-value: 7.2E-17
score: 61.3
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 135..147
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 22..54
IPR018451NAF/FISL domainPROSITEPS50816NAFcoord: 302..327
score: 12.470862
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 13..284

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G30360.1CSPI03G30360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007165 signal transduction
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity