CSPI03G29900 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G29900
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionnuclear envelope integral membrane protein 1 isoform X1
LocationChr3: 27393036 .. 27394451 (-)
RNA-Seq ExpressionCSPI03G29900
SyntenyCSPI03G29900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCCATTCAATGTTGTTTCGAGCTTCTACTTGCCTTGTATTTCTTTCATTGTTCTTCGCTTCAGCCTACTCCACTCCTGATCCTGAAGAGCTTCGTCTTCTTGTCTCTGAACCCACAATTATACAACTATTCCGTTACTTGCCTGTGGAAAATTCTTCAAGTTCCAGGCTTGGAACTGTGACGCTTTATGAAAGAGTACACATCCAAGGATTGCAGAGGTTTCTAAATCTTAAGAAGGTTGCACACACAGTGACAGTGAAGGTTTCAATGAAAAGTTCAAGTTCTAGGACATCAAATTTTTATGTTTGTTTTCACAAGAATACATCACTTGGGATAGGAATGTGTCCTCAAAGTCAATGGGAGAAAGCTTTCGAAGGTTCTTGGGCTCAATTCATGTCACCATTTGACCACAGGATTTTAGATATTAGAACATCTGGGTTATCTTTAGAAACTTTTGAAGTATCAATTGAAGAAGAATTCTCTCGATATCGCATAATCTTTTTAATCCTGGGTGTAGTGTTGATGAGTTCAGCCTCCATACTAAGCAAGTTGTTGGTATTCTATTTGGGCGGTGGTTGGCTGATTAGATTTCTCCTTCTACTGTTAATGATCCTTTCTCAGAGAATGAAGCTTCTTTCTCGTCGTGGGAAGAACTCACTTCAAATATTTCTATATGCTTACGCATCTGTAGGTTGTTTGGGATCTTTTTTCCTTCATTATGTTCTTGATCTATTGAATCAAATTGTTTTGGAAATGGGTATAACTATAAGTCAGGACATGTTTGATCCTCTAGCACTTGCAACATTCCTGATTGCTATTATTTTACCGATTGGAACGTGGTTGGGCTTTTGGGTAGCCCACAAATTTGTGGATCGTGAAAATGGATTAATTGAAAAAAACATATCACATTTTGTCGTATCGACATCCATTCAAATTTTGGCTACTTTTCTTATTCTTAAGTGTTCCTTGGATCCCATCTTGGCAACAGGAGGTCTAATATGTGGGACAATGGCTTCAATAATGACGAGCAATATCTTTAAGTTCCAATTGAATTTCTTACAATCTCCAAATGAAACGTCCAACCATTTGGTTGAATACAGGTTGAGGACTGATTTGCTTCAGCACCGCAGTTCATTTACACATGATGATGATGTATATCCTTCAATGTTCCATTCGACACATGAACGGAGAAAGATTTCCAAGGATGAGTGGGAAAGGCTCACCAAAGACTCAACTAAAAAGGCTTTGGAAGAACTAGTTTCTTCCTCGGGTTTTACTAGATGGCTTCTTGATAATGCGGGTAGAATCAACATACCTCCTCTTCAAGGAGTAGAGTTGGAAAGTGTCGAAAGTGACTCTATTGGTTCTGATCAGTTGCCTTTGTCGACAACCGACAAAGTGAGTTTTACTTAA

mRNA sequence

ATGATCCATTCAATGTTGTTTCGAGCTTCTACTTGCCTTGTATTTCTTTCATTGTTCTTCGCTTCAGCCTACTCCACTCCTGATCCTGAAGAGCTTCGTCTTCTTGTCTCTGAACCCACAATTATACAACTATTCCGTTACTTGCCTGTGGAAAATTCTTCAAGTTCCAGGCTTGGAACTGTGACGCTTTATGAAAGAGTACACATCCAAGGATTGCAGAGGTTTCTAAATCTTAAGAAGGTTGCACACACAGTGACAGTGAAGGTTTCAATGAAAAGTTCAAGTTCTAGGACATCAAATTTTTATGTTTGTTTTCACAAGAATACATCACTTGGGATAGGAATGTGTCCTCAAAGTCAATGGGAGAAAGCTTTCGAAGGTTCTTGGGCTCAATTCATGTCACCATTTGACCACAGGATTTTAGATATTAGAACATCTGGGTTATCTTTAGAAACTTTTGAAGTATCAATTGAAGAAGAATTCTCTCGATATCGCATAATCTTTTTAATCCTGGGTGTAGTGTTGATGAGTTCAGCCTCCATACTAAGCAAGTTGTTGGTATTCTATTTGGGCGGTGGTTGGCTGATTAGATTTCTCCTTCTACTGTTAATGATCCTTTCTCAGAGAATGAAGCTTCTTTCTCGTCGTGGGAAGAACTCACTTCAAATATTTCTATATGCTTACGCATCTGTAGGTTGTTTGGGATCTTTTTTCCTTCATTATGTTCTTGATCTATTGAATCAAATTGTTTTGGAAATGGGTATAACTATAAGTCAGGACATGTTTGATCCTCTAGCACTTGCAACATTCCTGATTGCTATTATTTTACCGATTGGAACGTGGTTGGGCTTTTGGGTAGCCCACAAATTTGTGGATCGTGAAAATGGATTAATTGAAAAAAACATATCACATTTTGTCGTATCGACATCCATTCAAATTTTGGCTACTTTTCTTATTCTTAAGTGTTCCTTGGATCCCATCTTGGCAACAGGAGGTCTAATATGTGGGACAATGGCTTCAATAATGACGAGCAATATCTTTAAGTTCCAATTGAATTTCTTACAATCTCCAAATGAAACGTCCAACCATTTGGTTGAATACAGGTTGAGGACTGATTTGCTTCAGCACCGCAGTTCATTTACACATGATGATGATGTATATCCTTCAATGTTCCATTCGACACATGAACGGAGAAAGATTTCCAAGGATGAGTGGGAAAGGCTCACCAAAGACTCAACTAAAAAGGCTTTGGAAGAACTAGTTTCTTCCTCGGGTTTTACTAGATGGCTTCTTGATAATGCGGGTAGAATCAACATACCTCCTCTTCAAGGAGTAGAGTTGGAAAGTGTCGAAAGTGACTCTATTGGTTCTGATCAGTTGCCTTTGTCGACAACCGACAAAGTGAGTTTTACTTAA

Coding sequence (CDS)

ATGATCCATTCAATGTTGTTTCGAGCTTCTACTTGCCTTGTATTTCTTTCATTGTTCTTCGCTTCAGCCTACTCCACTCCTGATCCTGAAGAGCTTCGTCTTCTTGTCTCTGAACCCACAATTATACAACTATTCCGTTACTTGCCTGTGGAAAATTCTTCAAGTTCCAGGCTTGGAACTGTGACGCTTTATGAAAGAGTACACATCCAAGGATTGCAGAGGTTTCTAAATCTTAAGAAGGTTGCACACACAGTGACAGTGAAGGTTTCAATGAAAAGTTCAAGTTCTAGGACATCAAATTTTTATGTTTGTTTTCACAAGAATACATCACTTGGGATAGGAATGTGTCCTCAAAGTCAATGGGAGAAAGCTTTCGAAGGTTCTTGGGCTCAATTCATGTCACCATTTGACCACAGGATTTTAGATATTAGAACATCTGGGTTATCTTTAGAAACTTTTGAAGTATCAATTGAAGAAGAATTCTCTCGATATCGCATAATCTTTTTAATCCTGGGTGTAGTGTTGATGAGTTCAGCCTCCATACTAAGCAAGTTGTTGGTATTCTATTTGGGCGGTGGTTGGCTGATTAGATTTCTCCTTCTACTGTTAATGATCCTTTCTCAGAGAATGAAGCTTCTTTCTCGTCGTGGGAAGAACTCACTTCAAATATTTCTATATGCTTACGCATCTGTAGGTTGTTTGGGATCTTTTTTCCTTCATTATGTTCTTGATCTATTGAATCAAATTGTTTTGGAAATGGGTATAACTATAAGTCAGGACATGTTTGATCCTCTAGCACTTGCAACATTCCTGATTGCTATTATTTTACCGATTGGAACGTGGTTGGGCTTTTGGGTAGCCCACAAATTTGTGGATCGTGAAAATGGATTAATTGAAAAAAACATATCACATTTTGTCGTATCGACATCCATTCAAATTTTGGCTACTTTTCTTATTCTTAAGTGTTCCTTGGATCCCATCTTGGCAACAGGAGGTCTAATATGTGGGACAATGGCTTCAATAATGACGAGCAATATCTTTAAGTTCCAATTGAATTTCTTACAATCTCCAAATGAAACGTCCAACCATTTGGTTGAATACAGGTTGAGGACTGATTTGCTTCAGCACCGCAGTTCATTTACACATGATGATGATGTATATCCTTCAATGTTCCATTCGACACATGAACGGAGAAAGATTTCCAAGGATGAGTGGGAAAGGCTCACCAAAGACTCAACTAAAAAGGCTTTGGAAGAACTAGTTTCTTCCTCGGGTTTTACTAGATGGCTTCTTGATAATGCGGGTAGAATCAACATACCTCCTCTTCAAGGAGTAGAGTTGGAAAGTGTCGAAAGTGACTCTATTGGTTCTGATCAGTTGCCTTTGTCGACAACCGACAAAGTGAGTTTTACTTAA

Protein sequence

MIHSMLFRASTCLVFLSLFFASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGTVTLYERVHIQGLQRFLNLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQWEKAFEGSWAQFMSPFDHRILDIRTSGLSLETFEVSIEEEFSRYRIIFLILGVVLMSSASILSKLLVFYLGGGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFFLHYVLDLLNQIVLEMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKFVDRENGLIEKNISHFVVSTSIQILATFLILKCSLDPILATGGLICGTMASIMTSNIFKFQLNFLQSPNETSNHLVEYRLRTDLLQHRSSFTHDDDVYPSMFHSTHERRKISKDEWERLTKDSTKKALEELVSSSGFTRWLLDNAGRINIPPLQGVELESVESDSIGSDQLPLSTTDKVSFT*
Homology
BLAST of CSPI03G29900 vs. ExPASy TrEMBL
Match: A0A0A0LD37 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G701570 PE=3 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 3.7e-258
Identity = 469/471 (99.58%), Postives = 469/471 (99.58%), Query Frame = 0

Query: 1   MIHSMLFRASTCLVFLSLFFASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGT 60
           MIHSMLFRASTCLVFLSLFFASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGT
Sbjct: 25  MIHSMLFRASTCLVFLSLFFASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGT 84

Query: 61  VTLYERVHIQGLQRFLNLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQ 120
           VTLYERVHIQGLQRFLNLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQ
Sbjct: 85  VTLYERVHIQGLQRFLNLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQ 144

Query: 121 WEKAFEGSWAQFMSPFDHRILDIRTSGLSLETFEVSIEEEFSRYRIIFLILGVVLMSSAS 180
           WEKAFEGSWAQFMSPFDHRILDIRTSGLSLETFEVSIEEEFSRYRIIFLILGVVLMSSAS
Sbjct: 145 WEKAFEGSWAQFMSPFDHRILDIRTSGLSLETFEVSIEEEFSRYRIIFLILGVVLMSSAS 204

Query: 181 ILSKLLVFYLGGGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFFLH 240
           ILSKLLVFYLGGGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFFLH
Sbjct: 205 ILSKLLVFYLGGGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFFLH 264

Query: 241 YVLDLLNQIVLEMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKFVDRENGLIEK 300
           YVLDLLNQIVLEMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKFVDRENGLIEK
Sbjct: 265 YVLDLLNQIVLEMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKFVDRENGLIEK 324

Query: 301 NISHFVVSTSIQILATFLILKCSLDPILATGGLICGTMASIMTSNIFKFQLNFLQSPNET 360
           NISHFVVSTSIQILATFLILKCSLDPILATGGLICGTMASIMTSNIFKFQLN LQSPNET
Sbjct: 325 NISHFVVSTSIQILATFLILKCSLDPILATGGLICGTMASIMTSNIFKFQLNLLQSPNET 384

Query: 361 SNHLVEYRLRTDLLQHRSSFTHDDDVYPSMFHSTHERRKISKDEWERLTKDSTKKALEEL 420
           SNHLVEYRLRTDLLQHRSSFTHDDDVYPSMFHSTHERRKISKDEWERLTKDSTKKALEEL
Sbjct: 385 SNHLVEYRLRTDLLQHRSSFTHDDDVYPSMFHSTHERRKISKDEWERLTKDSTKKALEEL 444

Query: 421 VSSSGFTRWLLDNAGRINIPPLQGVELESVESDSIGSDQLPLSTTDKVSFT 472
           VSSSGFTRWLLDNAGRINIPPLQGVELESVESDSIGSDQL LSTTDKVSFT
Sbjct: 445 VSSSGFTRWLLDNAGRINIPPLQGVELESVESDSIGSDQLSLSTTDKVSFT 495

BLAST of CSPI03G29900 vs. ExPASy TrEMBL
Match: A0A1S3CFC0 (uncharacterized protein LOC103500297 OS=Cucumis melo OX=3656 GN=LOC103500297 PE=3 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 2.4e-132
Identity = 276/463 (59.61%), Postives = 334/463 (72.14%), Query Frame = 0

Query: 2   IHSMLFRASTCLVFLSLFFASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGTV 61
           IHS LF+ASTCLVFL++FF SAYST DP ELRL+VS+ T IQL R LPV+NS  S   T+
Sbjct: 59  IHSSLFQASTCLVFLAVFFVSAYSTSDPGELRLVVSKLTTIQLSRGLPVKNSPGSIPETL 118

Query: 62  TLYERVHIQGLQRFLNLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQW 121
            + ERV+IQGL RF NL+KVAHTV VKVSM++SS R     VCFHK  S G+GMCP++QW
Sbjct: 119 MVCERVYIQGLPRFQNLRKVAHTVRVKVSMRNSSFRIPKVEVCFHKKMSFGLGMCPENQW 178

Query: 122 EKAFEGSWAQFMSPFDHRILDIRTSGLSLETFEVSIEEEFSRYRIIFLILGVVLMSSASI 181
           EK F+GSWA+ MSPFD  ILDIRT GLSLE+FEVS+EEEFS+YRII+LI+GVVLMSSAS 
Sbjct: 179 EKIFKGSWARSMSPFDDNILDIRTPGLSLESFEVSLEEEFSQYRIIYLIMGVVLMSSAST 238

Query: 182 LSKLLVFYLGGGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFFLHY 241
           +SK LVFY G   +I  LL++LMIL Q M+LL    KNSL  FL+ YA   CL SF+ HY
Sbjct: 239 MSKSLVFYYGSSMVIGILLVVLMILFQGMRLLPTARKNSLGFFLFTYAV--CLVSFYCHY 298

Query: 242 VLDLLNQIVLEMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKFVDRENGLIEKN 301
           +L LL+ ++LEMG  IS+DM +PLAL  FL+A I  IGTWLGF V HK+V  ++GL++  
Sbjct: 299 ILGLLDHVLLEMG--ISKDMLNPLALKIFLLAFIFLIGTWLGFCVVHKYVHHDDGLVDST 358

Query: 302 ISHFVVSTSIQILATFLILKCSLDPILATGGLICGTMASIMTSNIFKFQL------NFLQ 361
           +S F VS  IQILATFLIL+CSLDP+LATG LICG + S M   IFKF+           
Sbjct: 359 VSLF-VSWFIQILATFLILQCSLDPLLATGVLICGLLTSSMLRRIFKFRFRRRLYKKLFI 418

Query: 362 SPNETS--NHLVEY---------------RLRTDLLQHRSSFTHDDDVYPSMFHSTHERR 421
           SPN+T   +H+++                +    L+Q  SSF  DDDVYPSMFHST ++R
Sbjct: 419 SPNKTPKISHMLDMPCLDAQDDQPRINRSQSNNLLIQSCSSFELDDDVYPSMFHSTPKQR 478

Query: 422 KISKDEWERLTKDSTKKALEELVSSSGFTRWLLDNAGRINIPP 442
           K SKDEWE+ TKDSTKKALE L+SS  F+RWL DNA RIN  P
Sbjct: 479 KFSKDEWEKFTKDSTKKALEGLISSPDFSRWLADNADRINTTP 516

BLAST of CSPI03G29900 vs. ExPASy TrEMBL
Match: A0A1S4E3G6 (nuclear envelope integral membrane protein 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500299 PE=3 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 1.4e-127
Identity = 284/475 (59.79%), Postives = 332/475 (69.89%), Query Frame = 0

Query: 1   MIHSMLFRASTCLVFLSLFFASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGT 60
           M+HS LFRASTCL+FLS+FFAS Y T DPE+ RL+VSE T IQL   LPV+NS  S+ GT
Sbjct: 63  MVHSKLFRASTCLLFLSVFFASVYCTSDPEDHRLIVSESTTIQLSGGLPVKNSPGSKPGT 122

Query: 61  VTLYERVHIQGLQRFLNLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQ 120
           V   ERV+IQGL RF NLKK AHTV VKVS ++SS   SN  VCFH+N SLGIGMCPQSQ
Sbjct: 123 VVACERVYIQGLPRFKNLKKAAHTVKVKVSSRNSSFWMSNVEVCFHRNMSLGIGMCPQSQ 182

Query: 121 WEKAFEGSWAQFMSPFDHRILDIRTSGLSLETFEVSIEEEFSRYRIIFLILGVVLMSSAS 180
           WEK   GSW Q MSPFDH++LDIRT GLSLE+FEVS E+EF  YRIIFLILGV+LMSSAS
Sbjct: 183 WEKVGRGSWVQSMSPFDHKLLDIRTCGLSLESFEVSTEDEFFLYRIIFLILGVLLMSSAS 242

Query: 181 ILSKLLVFYLGGGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFFLH 240
           ILSK LVFY G G  I  LL++LMIL Q MKLL    K+SL IFLYA ++VG LGSFF+ 
Sbjct: 243 ILSKSLVFYYGSGMAIGILLIVLMILFQGMKLLPTGRKSSLVIFLYA-SAVG-LGSFFIR 302

Query: 241 YVLDLLNQIVLEMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKFVDRENGLIEK 300
           Y+  LL QI++EMG  IS+DM++P  LA FL+A I  IG WLGFWV HKFV  E+G I+ 
Sbjct: 303 YIPGLLYQILMEMG--ISEDMYNP--LAAFLLAFIFLIGAWLGFWVVHKFVLDEDGSIDT 362

Query: 301 NISHFVVSTSIQILATFLILKCSLDPILATGGLICGTMASIMTSNIFKFQL------NFL 360
           + S F V+ SI+ILA+ LIL+CSLDP+LATG LICG +AS M   IFKF+       N  
Sbjct: 363 STSLF-VTWSIRILASLLILQCSLDPLLATGVLICGIVASSMLRKIFKFRFLRRLFKNLF 422

Query: 361 QSPNE--TSNHLVE------------------------YRL--RTDLLQHRSSFTHDDDV 420
           +SP +    +H+ +                        YR   R  LLQ   S  H  DV
Sbjct: 423 KSPKKIPKRSHISDMPRWDDSDDECTLKTTPLYKEPRFYRSQNRKFLLQSCDSSKH-GDV 482

Query: 421 YPSMFHSTHERRKISKDEWERLTKDSTKKALEELVSSSGFTRWLLDNAGRINIPP 442
           YPS FHST ERRK SKDEWE+ TKDSTKKALE LVSS  F+ WL+D A RI+I P
Sbjct: 483 YPSTFHSTPERRKFSKDEWEKFTKDSTKKALEGLVSSPDFSSWLVDRADRISITP 529

BLAST of CSPI03G29900 vs. ExPASy TrEMBL
Match: A0A6J1G8K0 (uncharacterized protein LOC111451852 OS=Cucurbita moschata OX=3662 GN=LOC111451852 PE=3 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 5.7e-126
Identity = 276/473 (58.35%), Postives = 335/473 (70.82%), Query Frame = 0

Query: 1   MIHSMLFRASTCLVFLSLFFASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGT 60
           M+ SMLFRASTCL+FL+LFFA AYS PDP++LRL+VSE T +QL R LPV+NS  S+ GT
Sbjct: 1   MVPSMLFRASTCLLFLALFFALAYSIPDPQQLRLVVSESTTLQLSRGLPVQNSPGSKPGT 60

Query: 61  VTLYERVHIQGLQRFLNLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQ 120
           V + ERV+IQGL R  NL K+AHTV VK+SM +SS+R  N  VCFH+N SLGIGMCPQSQ
Sbjct: 61  VVVCERVYIQGLLRIKNLWKLAHTVKVKLSMINSSARIPNVEVCFHRNMSLGIGMCPQSQ 120

Query: 121 WEKAFEGSWAQFMSPFDHRILDIRTSGLSLETFEVSIEEEFSRYRIIFLILGVVLMSSAS 180
           WEK  +GSWAQ MSPFDH+++DIRTSGLSLE+FEVSIEEEF  YRI+FLILG+VLMSSAS
Sbjct: 121 WEKVAKGSWAQSMSPFDHKLVDIRTSGLSLESFEVSIEEEFFMYRIVFLILGLVLMSSAS 180

Query: 181 ILSKLLVFYLGGGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFFLH 240
           IL K LVFY G    I  LL++LMIL Q MKLL    K+SL IFLYA ++VG LGSFFL 
Sbjct: 181 ILGKSLVFYYGSAMTIGVLLVVLMILFQGMKLLPTGRKSSLAIFLYA-SAVG-LGSFFLR 240

Query: 241 YVLDLLNQIVLEMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKFVDRENGLIEK 300
           Y+  LL+QI+LEMG  IS+DM++P  +ATFL+A I  IG WLGFWV HKFV  E+G I+ 
Sbjct: 241 YIPGLLHQILLEMG--ISEDMYNP--VATFLLAFIFLIGAWLGFWVVHKFVLDEDGSIDT 300

Query: 301 NISHFVVSTSIQILATFLILKCSLDPILATGGLICGTMASIMTSNIFKFQL------NFL 360
           + S F V+  I+ILA  LIL+CSLDP+LATG LICG MAS +    FK +       N  
Sbjct: 301 STSLF-VTWCIRILAALLILQCSLDPLLATGVLICGIMASSILRRTFKLRFLRRQYKNLF 360

Query: 361 QSPNE--TSNHLVE---YRLRTDLLQHRSSFTHDD---------------------DVYP 420
           +SP      +H+ +   +    D L  +S  +++D                     D YP
Sbjct: 361 KSPKRKLKRSHISDKPRFDDSDDELAMKSPLSYEDPGFYRSQDRNFALQSCSLSERDEYP 420

Query: 421 SMFHSTHERRKISKDEWERLTKDSTKKALEELVSSSGFTRWLLDNAGRINIPP 442
           S FHST  RR+ SKDEW+R TKDST+KALE LVSS  F RWL+DNA RI+I P
Sbjct: 421 STFHSTPGRREFSKDEWKRFTKDSTEKALEGLVSSPDFGRWLVDNADRISITP 466

BLAST of CSPI03G29900 vs. ExPASy TrEMBL
Match: A0A6J1L1F6 (uncharacterized protein LOC111499541 OS=Cucurbita maxima OX=3661 GN=LOC111499541 PE=3 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 7.4e-126
Identity = 277/473 (58.56%), Postives = 333/473 (70.40%), Query Frame = 0

Query: 1   MIHSMLFRASTCLVFLSLFFASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGT 60
           M+ S LFRASTCL+FL+LFFA AYS PDP+ LRL+VSE T +QL R LPV+NS  S+ GT
Sbjct: 1   MVPSTLFRASTCLLFLALFFALAYSIPDPQPLRLVVSESTTLQLSRGLPVQNSPGSKPGT 60

Query: 61  VTLYERVHIQGLQRFLNLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQ 120
           V + ERV+IQGL R  NL K+AHTV VK+SM +SS+R  N  VCFH+N SLGIGMCPQSQ
Sbjct: 61  VVVCERVYIQGLLRIKNLWKLAHTVKVKLSMINSSARIPNLEVCFHRNMSLGIGMCPQSQ 120

Query: 121 WEKAFEGSWAQFMSPFDHRILDIRTSGLSLETFEVSIEEEFSRYRIIFLILGVVLMSSAS 180
           WEK  +GSWAQ MSPFDH+++DIRTSGLSLE+FEVSIEEEF  YRIIFLILG+VLMSSAS
Sbjct: 121 WEKVAKGSWAQSMSPFDHKLIDIRTSGLSLESFEVSIEEEFFMYRIIFLILGLVLMSSAS 180

Query: 181 ILSKLLVFYLGGGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFFLH 240
           IL K LVFY G    I  LL++LMIL Q MKLL    K+SL IFLYA ++VG LGSFFL 
Sbjct: 181 ILGKSLVFYYGSAMTIGVLLVVLMILFQGMKLLPTGRKSSLAIFLYA-SAVG-LGSFFLR 240

Query: 241 YVLDLLNQIVLEMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKFVDRENGLIEK 300
           Y+  LL+QI+LEMG  IS+DM++P  +ATFL+A I  IG WLGFWV HKFV  E+G I+ 
Sbjct: 241 YIPGLLHQILLEMG--ISEDMYNP--VATFLLAFIFLIGAWLGFWVVHKFVLDEDGSIDT 300

Query: 301 NISHFVVSTSIQILATFLILKCSLDPILATGGLICGTMASIMTSNIFKFQL------NFL 360
           + S F V+  I+ILA  LIL+CSLDP+LATG LICG MAS +    FK +       N  
Sbjct: 301 STSLF-VTWCIRILAALLILQCSLDPLLATGVLICGIMASSILRRTFKLRFLRRQYKNLF 360

Query: 361 QSPNE--TSNHLVE---YRLRTDLLQHRSSFTHDD---------------------DVYP 420
           +SP      +H+ +   +    D L  +S  +++D                     D YP
Sbjct: 361 KSPKRKLKRSHISDTPRFDDSDDELAMKSPLSYEDPKFYGSQDRNFALQSCSLSERDEYP 420

Query: 421 SMFHSTHERRKISKDEWERLTKDSTKKALEELVSSSGFTRWLLDNAGRINIPP 442
           S FHST  RR+ SKDEW+R TKDST+KALE LVSS  F RWL+DNA RINI P
Sbjct: 421 STFHSTPGRREFSKDEWKRFTKDSTEKALEGLVSSPDFGRWLVDNADRINITP 466

BLAST of CSPI03G29900 vs. NCBI nr
Match: XP_011652833.2 (uncharacterized protein LOC105435121 [Cucumis sativus] >KAE8650939.1 hypothetical protein Csa_001779 [Cucumis sativus])

HSP 1 Score: 903.3 bits (2333), Expect = 8.9e-259
Identity = 470/471 (99.79%), Postives = 470/471 (99.79%), Query Frame = 0

Query: 1   MIHSMLFRASTCLVFLSLFFASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGT 60
           MIHSMLFRASTCLVFLSLFFASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGT
Sbjct: 25  MIHSMLFRASTCLVFLSLFFASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGT 84

Query: 61  VTLYERVHIQGLQRFLNLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQ 120
           VTLYERVHIQGLQRFLNLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQ
Sbjct: 85  VTLYERVHIQGLQRFLNLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQ 144

Query: 121 WEKAFEGSWAQFMSPFDHRILDIRTSGLSLETFEVSIEEEFSRYRIIFLILGVVLMSSAS 180
           WEKAFEGSWAQFMSPFDHRILDIRTSGLSLETFEVSIEEEFSRYRIIFLILGVVLMSSAS
Sbjct: 145 WEKAFEGSWAQFMSPFDHRILDIRTSGLSLETFEVSIEEEFSRYRIIFLILGVVLMSSAS 204

Query: 181 ILSKLLVFYLGGGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFFLH 240
           ILSKLLVFYLGGGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFFLH
Sbjct: 205 ILSKLLVFYLGGGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFFLH 264

Query: 241 YVLDLLNQIVLEMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKFVDRENGLIEK 300
           YVLDLLNQIVLEMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKFVDRENGLIEK
Sbjct: 265 YVLDLLNQIVLEMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKFVDRENGLIEK 324

Query: 301 NISHFVVSTSIQILATFLILKCSLDPILATGGLICGTMASIMTSNIFKFQLNFLQSPNET 360
           NISHFVVSTSIQILATFLILKCSLDPILATGGLICGTMASIMTSNIFKFQLN LQSPNET
Sbjct: 325 NISHFVVSTSIQILATFLILKCSLDPILATGGLICGTMASIMTSNIFKFQLNLLQSPNET 384

Query: 361 SNHLVEYRLRTDLLQHRSSFTHDDDVYPSMFHSTHERRKISKDEWERLTKDSTKKALEEL 420
           SNHLVEYRLRTDLLQHRSSFTHDDDVYPSMFHSTHERRKISKDEWERLTKDSTKKALEEL
Sbjct: 385 SNHLVEYRLRTDLLQHRSSFTHDDDVYPSMFHSTHERRKISKDEWERLTKDSTKKALEEL 444

Query: 421 VSSSGFTRWLLDNAGRINIPPLQGVELESVESDSIGSDQLPLSTTDKVSFT 472
           VSSSGFTRWLLDNAGRINIPPLQGVELESVESDSIGSDQLPLSTTDKVSFT
Sbjct: 445 VSSSGFTRWLLDNAGRINIPPLQGVELESVESDSIGSDQLPLSTTDKVSFT 495

BLAST of CSPI03G29900 vs. NCBI nr
Match: XP_008461773.1 (PREDICTED: uncharacterized protein LOC103500297 [Cucumis melo])

HSP 1 Score: 482.3 bits (1240), Expect = 4.9e-132
Identity = 276/463 (59.61%), Postives = 334/463 (72.14%), Query Frame = 0

Query: 2   IHSMLFRASTCLVFLSLFFASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGTV 61
           IHS LF+ASTCLVFL++FF SAYST DP ELRL+VS+ T IQL R LPV+NS  S   T+
Sbjct: 59  IHSSLFQASTCLVFLAVFFVSAYSTSDPGELRLVVSKLTTIQLSRGLPVKNSPGSIPETL 118

Query: 62  TLYERVHIQGLQRFLNLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQW 121
            + ERV+IQGL RF NL+KVAHTV VKVSM++SS R     VCFHK  S G+GMCP++QW
Sbjct: 119 MVCERVYIQGLPRFQNLRKVAHTVRVKVSMRNSSFRIPKVEVCFHKKMSFGLGMCPENQW 178

Query: 122 EKAFEGSWAQFMSPFDHRILDIRTSGLSLETFEVSIEEEFSRYRIIFLILGVVLMSSASI 181
           EK F+GSWA+ MSPFD  ILDIRT GLSLE+FEVS+EEEFS+YRII+LI+GVVLMSSAS 
Sbjct: 179 EKIFKGSWARSMSPFDDNILDIRTPGLSLESFEVSLEEEFSQYRIIYLIMGVVLMSSAST 238

Query: 182 LSKLLVFYLGGGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFFLHY 241
           +SK LVFY G   +I  LL++LMIL Q M+LL    KNSL  FL+ YA   CL SF+ HY
Sbjct: 239 MSKSLVFYYGSSMVIGILLVVLMILFQGMRLLPTARKNSLGFFLFTYAV--CLVSFYCHY 298

Query: 242 VLDLLNQIVLEMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKFVDRENGLIEKN 301
           +L LL+ ++LEMG  IS+DM +PLAL  FL+A I  IGTWLGF V HK+V  ++GL++  
Sbjct: 299 ILGLLDHVLLEMG--ISKDMLNPLALKIFLLAFIFLIGTWLGFCVVHKYVHHDDGLVDST 358

Query: 302 ISHFVVSTSIQILATFLILKCSLDPILATGGLICGTMASIMTSNIFKFQL------NFLQ 361
           +S F VS  IQILATFLIL+CSLDP+LATG LICG + S M   IFKF+           
Sbjct: 359 VSLF-VSWFIQILATFLILQCSLDPLLATGVLICGLLTSSMLRRIFKFRFRRRLYKKLFI 418

Query: 362 SPNETS--NHLVEY---------------RLRTDLLQHRSSFTHDDDVYPSMFHSTHERR 421
           SPN+T   +H+++                +    L+Q  SSF  DDDVYPSMFHST ++R
Sbjct: 419 SPNKTPKISHMLDMPCLDAQDDQPRINRSQSNNLLIQSCSSFELDDDVYPSMFHSTPKQR 478

Query: 422 KISKDEWERLTKDSTKKALEELVSSSGFTRWLLDNAGRINIPP 442
           K SKDEWE+ TKDSTKKALE L+SS  F+RWL DNA RIN  P
Sbjct: 479 KFSKDEWEKFTKDSTKKALEGLISSPDFSRWLADNADRINTTP 516

BLAST of CSPI03G29900 vs. NCBI nr
Match: XP_038906035.1 (uncharacterized protein LOC120091936 [Benincasa hispida])

HSP 1 Score: 478.8 bits (1231), Expect = 5.4e-131
Identity = 287/475 (60.42%), Postives = 346/475 (72.84%), Query Frame = 0

Query: 1   MIHSMLFRASTCLVFLSLFFASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGT 60
           M+HS LFRASTCL+FL++FFASAYST DPEELRL+VS+ T IQL    PV++S  SR GT
Sbjct: 63  MVHSTLFRASTCLLFLAVFFASAYSTSDPEELRLVVSKSTTIQLSGGFPVKSSPGSRPGT 122

Query: 61  VTLYERVHIQGLQRFLNLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQ 120
           V + ERV+IQGL RF NL+KVAHTV VKVSM++SSSR  N  VCFH+N SLG+GMCPQSQ
Sbjct: 123 VVVCERVYIQGLPRFKNLRKVAHTVKVKVSMRNSSSRIPNVEVCFHRNMSLGMGMCPQSQ 182

Query: 121 WEKAFEGSWAQFMSPFDHRILDIRTSGLSLETFEVSIEEEFSRYRIIFLILGVVLMSSAS 180
           W+K  +GSW Q MSPFDH++LDIRTSGLSLE+FEVSIEEEF  YRIIFLILG+VLMSSAS
Sbjct: 183 WQKVAKGSWVQSMSPFDHKLLDIRTSGLSLESFEVSIEEEFFLYRIIFLILGMVLMSSAS 242

Query: 181 ILSKLLVFYLGGGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFFLH 240
           ILSK LVFY G    I  LL++LMIL Q MKLL    K+SL IFLYA ++VG LGSFFL 
Sbjct: 243 ILSKSLVFYYGSAMAIGILLVVLMILFQGMKLLPTGRKSSLAIFLYA-SAVG-LGSFFLR 302

Query: 241 YVLDLLNQIVLEMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKFVDRENGLIEK 300
           Y+  LL+QI+LEMG  IS+DM++P  LA FL+A I  IG WLGFWV HKFV  ++G I+ 
Sbjct: 303 YIPGLLHQILLEMG--ISEDMYNP--LAAFLLAFIFLIGAWLGFWVVHKFVLDKDGSIDT 362

Query: 301 NISHFVVSTSIQILATFLILKCSLDPILATGGLICGTMASIMTSNIFKFQL------NFL 360
           + S F V+ SI+I+AT LIL+CSLDP+L+TG LICG +AS M   IFKF+       N  
Sbjct: 363 STSLF-VTWSIRIVATLLILQCSLDPLLSTGVLICGIVASSMLRKIFKFRFLRRLYKNLF 422

Query: 361 QSPNET---SNHLV----------EYRLRTDLL---------QHRS------SFTHDDDV 420
           +SP +T    +H+           E  L+T  L         Q+R+      S +   DV
Sbjct: 423 KSPKKTLKRRSHISDMPRLEDPDDECMLKTPPLYEDPRFYSSQYRNFSLQSCSSSKRGDV 482

Query: 421 YPSMFHSTHERRKISKDEWERLTKDSTKKALEELVSSSGFTRWLLDNAGRINIPP 442
           YPS FHST ERRK SK+EWE+ TKDSTKKALE LVSS  F+RWL+D+A RI+I P
Sbjct: 483 YPSTFHSTPERRKFSKEEWEKFTKDSTKKALEGLVSSPDFSRWLVDSADRISITP 530

BLAST of CSPI03G29900 vs. NCBI nr
Match: XP_016902769.1 (PREDICTED: nuclear envelope integral membrane protein 1 isoform X1 [Cucumis melo])

HSP 1 Score: 466.5 bits (1199), Expect = 2.8e-127
Identity = 284/475 (59.79%), Postives = 332/475 (69.89%), Query Frame = 0

Query: 1   MIHSMLFRASTCLVFLSLFFASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGT 60
           M+HS LFRASTCL+FLS+FFAS Y T DPE+ RL+VSE T IQL   LPV+NS  S+ GT
Sbjct: 63  MVHSKLFRASTCLLFLSVFFASVYCTSDPEDHRLIVSESTTIQLSGGLPVKNSPGSKPGT 122

Query: 61  VTLYERVHIQGLQRFLNLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQ 120
           V   ERV+IQGL RF NLKK AHTV VKVS ++SS   SN  VCFH+N SLGIGMCPQSQ
Sbjct: 123 VVACERVYIQGLPRFKNLKKAAHTVKVKVSSRNSSFWMSNVEVCFHRNMSLGIGMCPQSQ 182

Query: 121 WEKAFEGSWAQFMSPFDHRILDIRTSGLSLETFEVSIEEEFSRYRIIFLILGVVLMSSAS 180
           WEK   GSW Q MSPFDH++LDIRT GLSLE+FEVS E+EF  YRIIFLILGV+LMSSAS
Sbjct: 183 WEKVGRGSWVQSMSPFDHKLLDIRTCGLSLESFEVSTEDEFFLYRIIFLILGVLLMSSAS 242

Query: 181 ILSKLLVFYLGGGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFFLH 240
           ILSK LVFY G G  I  LL++LMIL Q MKLL    K+SL IFLYA ++VG LGSFF+ 
Sbjct: 243 ILSKSLVFYYGSGMAIGILLIVLMILFQGMKLLPTGRKSSLVIFLYA-SAVG-LGSFFIR 302

Query: 241 YVLDLLNQIVLEMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKFVDRENGLIEK 300
           Y+  LL QI++EMG  IS+DM++P  LA FL+A I  IG WLGFWV HKFV  E+G I+ 
Sbjct: 303 YIPGLLYQILMEMG--ISEDMYNP--LAAFLLAFIFLIGAWLGFWVVHKFVLDEDGSIDT 362

Query: 301 NISHFVVSTSIQILATFLILKCSLDPILATGGLICGTMASIMTSNIFKFQL------NFL 360
           + S F V+ SI+ILA+ LIL+CSLDP+LATG LICG +AS M   IFKF+       N  
Sbjct: 363 STSLF-VTWSIRILASLLILQCSLDPLLATGVLICGIVASSMLRKIFKFRFLRRLFKNLF 422

Query: 361 QSPNE--TSNHLVE------------------------YRL--RTDLLQHRSSFTHDDDV 420
           +SP +    +H+ +                        YR   R  LLQ   S  H  DV
Sbjct: 423 KSPKKIPKRSHISDMPRWDDSDDECTLKTTPLYKEPRFYRSQNRKFLLQSCDSSKH-GDV 482

Query: 421 YPSMFHSTHERRKISKDEWERLTKDSTKKALEELVSSSGFTRWLLDNAGRINIPP 442
           YPS FHST ERRK SKDEWE+ TKDSTKKALE LVSS  F+ WL+D A RI+I P
Sbjct: 483 YPSTFHSTPERRKFSKDEWEKFTKDSTKKALEGLVSSPDFSSWLVDRADRISITP 529

BLAST of CSPI03G29900 vs. NCBI nr
Match: XP_011651793.1 (uncharacterized protein LOC101210632 isoform X1 [Cucumis sativus])

HSP 1 Score: 464.9 bits (1195), Expect = 8.1e-127
Identity = 282/475 (59.37%), Postives = 331/475 (69.68%), Query Frame = 0

Query: 1   MIHSMLFRASTCLVFLSLFFASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGT 60
           M+HS LFRASTCL+FL++FFAS Y T DPE+ RL+VSE T IQL   LPV+NS  SR GT
Sbjct: 63  MVHSTLFRASTCLLFLAVFFASVYCTSDPEDHRLIVSESTTIQLSGGLPVKNSPGSRPGT 122

Query: 61  VTLYERVHIQGLQRFLNLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQ 120
           V   ERV+IQGL RF NLKKVAHTV VKVS+++SS   SN  VCFH+N SLGIGMCPQSQ
Sbjct: 123 VVACERVYIQGLPRFKNLKKVAHTVKVKVSLRNSSFGMSNVEVCFHRNMSLGIGMCPQSQ 182

Query: 121 WEKAFEGSWAQFMSPFDHRILDIRTSGLSLETFEVSIEEEFSRYRIIFLILGVVLMSSAS 180
           WEK   GSW Q  SPFDH++LDIRT G+SLE+FEVS EEEF  YRIIFLILGV+LMSSAS
Sbjct: 183 WEKVDRGSWVQSTSPFDHKLLDIRTRGISLESFEVSTEEEFFLYRIIFLILGVLLMSSAS 242

Query: 181 ILSKLLVFYLGGGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFFLH 240
           ILSK LVFY G G  I  LL++LMIL Q MKLL    K+SL IFLYA ++VG LGSFFL 
Sbjct: 243 ILSKSLVFYYGSGMAIGILLIVLMILFQGMKLLPTGRKSSLVIFLYA-SAVG-LGSFFLR 302

Query: 241 YVLDLLNQIVLEMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKFVDRENGLIEK 300
           Y+  LL QI+LEMG  IS+DM++P  LA FL+A I  +G WLGFWV HKF+  E+G I  
Sbjct: 303 YISGLLYQILLEMG--ISEDMYNP--LAAFLLAFIFLVGAWLGFWVVHKFILDEDGSINT 362

Query: 301 NISHFVVSTSIQILATFLILKCSLDPILATGGLICGTMASIMTSNIFKFQL------NFL 360
           + S F V+ SI+ILA+ LIL+CS+DP+LATG LICG +AS M   IFKF+       N  
Sbjct: 363 STSLF-VTWSIRILASLLILQCSVDPLLATGVLICGIVASSMLRKIFKFRFLRRLFKNLF 422

Query: 361 QSPNE--TSNHLV----------EYRLRTD----------------LLQHRSSFTHDDDV 420
           +SP +    +H+           E  L+T                 LLQ   S  H  DV
Sbjct: 423 KSPKKIPKKSHISDMPHLDDSDDECTLKTTPLYKEPRFFGSQNKKFLLQPCHSSKH-SDV 482

Query: 421 YPSMFHSTHERRKISKDEWERLTKDSTKKALEELVSSSGFTRWLLDNAGRINIPP 442
           YPS FHST ERR  SKDEWE+ TKDSTKKALE LVSS  F+ WL+D A RI+I P
Sbjct: 483 YPSTFHSTSERRNFSKDEWEKFTKDSTKKALEGLVSSPDFSSWLVDRADRISITP 529

BLAST of CSPI03G29900 vs. TAIR 10
Match: AT5G67610.1 (Uncharacterized conserved protein (DUF2215) )

HSP 1 Score: 266.9 bits (681), Expect = 3.0e-71
Identity = 183/469 (39.02%), Postives = 264/469 (56.29%), Query Frame = 0

Query: 13  LVFLSLF-FASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGTVTLYERVHIQG 72
           ++ L LF FAS  S+   +EL+ +V E   +Q+   L V+ S   +    +L ER+HI G
Sbjct: 16  IILLHLFTFASLVSS---DELQFVVGESGELQVTPSLEVKGSPGLKPDRTSLCERIHIHG 75

Query: 73  LQRFLNLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQWEKAFEGSWAQ 132
           L RF +L K AH++ + V+  S S +T+N  VCFH+N S GIGMCP S+WEKA +GSW Q
Sbjct: 76  LGRFKHLDKYAHSLKLIVN-ASISGKTNNIDVCFHRNLSRGIGMCPHSRWEKASKGSWVQ 135

Query: 133 FMSPFDHRILDIRTSGLSLETFEVSIEEEFSRYRIIFLILGVVLMSSASILSKLLVFYLG 192
            MSPFDH+ILD+R    +  + EVS  EE   +RI+FL+LG VL++SAS LS+ L FY  
Sbjct: 136 TMSPFDHKILDVRVPSSNKVSLEVSAVEELFMHRIVFLLLGAVLLASASTLSQSLAFYYS 195

Query: 193 GGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFFLHYVLDLLNQIVL 252
               +  +L++L++L Q MKLL   G++S  +F+  Y+++  LG F L Y+  L   ++ 
Sbjct: 196 SAMAVGIILVVLLVLFQGMKLLP-TGRSSFALFI--YSTLLGLGGFLLRYLPGLFESLLT 255

Query: 253 EMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKFVDRENGLIEKNISHFVVSTSI 312
           EMG  I ++M+ P A+  F+ A +   G + GFW   K +  E+G I+ + S F VS SI
Sbjct: 256 EMG--IDEEMYTPAAI--FVGAFLSLGGAFFGFWTVRKLILTEDGSIDVSTSLF-VSWSI 315

Query: 313 QILATFLILKCSLDPILATGGLICGTMASIMTSNI--FKFQLNFLQSPNETSNHLVEYRL 372
           +I+A  LIL+ S+DP+LA G LI   + S     I   KF L   + P      + E   
Sbjct: 316 RIMAAVLILQSSVDPLLAGGALISVILMSSTLKKITRLKFLLRLFEIPLNLLLGIWEAIR 375

Query: 373 RTDLLQ----------------------------------HRSSFTHDDDVYPSMFHSTH 432
            TD+                                     R S   + D +PS FH T 
Sbjct: 376 DTDIPSVPGYLHDFMQKSPDASGFRNRVTSASPSGGINNGMRESPPSESDTFPSSFHKTP 435

Query: 433 ERRKISKDEWERLTKDSTKKALEELVSSSGFTRWLLDNAGRINIPPLQG 445
           ER +++K+EW++LTKDST KA++ELVSS  F +W   NA RIN+ P +G
Sbjct: 436 ERSQLTKEEWKKLTKDSTTKAVQELVSSPDFGKWAAVNADRINVTPRKG 472

BLAST of CSPI03G29900 vs. TAIR 10
Match: AT5G67610.2 (Uncharacterized conserved protein (DUF2215) )

HSP 1 Score: 260.0 bits (663), Expect = 3.7e-69
Identity = 174/446 (39.01%), Postives = 250/446 (56.05%), Query Frame = 0

Query: 35  LVSEPTIIQLFRYLPVENSSSSRLGTVTLYERVHIQGLQRFLNLKKVAHTVTVKVSMKSS 94
           +V E   +Q+   L V+ S   +    +L ER+HI GL RF +L K AH++ + V+  S 
Sbjct: 48  VVGESGELQVTPSLEVKGSPGLKPDRTSLCERIHIHGLGRFKHLDKYAHSLKLIVN-ASI 107

Query: 95  SSRTSNFYVCFHKNTSLGIGMCPQSQWEKAFEGSWAQFMSPFDHRILDIRTSGLSLETFE 154
           S +T+N  VCFH+N S GIGMCP S+WEKA +GSW Q MSPFDH+ILD+R    +  + E
Sbjct: 108 SGKTNNIDVCFHRNLSRGIGMCPHSRWEKASKGSWVQTMSPFDHKILDVRVPSSNKVSLE 167

Query: 155 VSIEEEFSRYRIIFLILGVVLMSSASILSKLLVFYLGGGWLIRFLLLLLMILSQRMKLLS 214
           VS  EE   +RI+FL+LG VL++SAS LS+ L FY      +  +L++L++L Q MKLL 
Sbjct: 168 VSAVEELFMHRIVFLLLGAVLLASASTLSQSLAFYYSSAMAVGIILVVLLVLFQGMKLLP 227

Query: 215 RRGKNSLQIFLYAYASVGCLGSFFLHYVLDLLNQIVLEMGITISQDMFDPLALATFLIAI 274
             G++S  +F+  Y+++  LG F L Y+  L   ++ EMG  I ++M+ P A+  F+ A 
Sbjct: 228 -TGRSSFALFI--YSTLLGLGGFLLRYLPGLFESLLTEMG--IDEEMYTPAAI--FVGAF 287

Query: 275 ILPIGTWLGFWVAHKFVDRENGLIEKNISHFVVSTSIQILATFLILKCSLDPILATGGLI 334
           +   G + GFW   K +  E+G I+ + S F VS SI+I+A  LIL+ S+DP+LA G LI
Sbjct: 288 LSLGGAFFGFWTVRKLILTEDGSIDVSTSLF-VSWSIRIMAAVLILQSSVDPLLAGGALI 347

Query: 335 CGTMASIMTSNI--FKFQLNFLQSPNETSNHLVEYRLRTDLLQ----------------- 394
              + S     I   KF L   + P      + E    TD+                   
Sbjct: 348 SVILMSSTLKKITRLKFLLRLFEIPLNLLLGIWEAIRDTDIPSVPGYLHDFMQKSPDASG 407

Query: 395 -----------------HRSSFTHDDDVYPSMFHSTHERRKISKDEWERLTKDSTKKALE 445
                             R S   + D +PS FH T ER +++K+EW++LTKDST KA++
Sbjct: 408 FRNRVTSASPSGGINNGMRESPPSESDTFPSSFHKTPERSQLTKEEWKKLTKDSTTKAVQ 467

BLAST of CSPI03G29900 vs. TAIR 10
Match: AT3G49840.1 (Uncharacterized conserved protein (DUF2215) )

HSP 1 Score: 250.8 bits (639), Expect = 2.2e-66
Identity = 171/457 (37.42%), Postives = 272/457 (59.52%), Query Frame = 0

Query: 4   SMLFRAST-CLVFLSLFFASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGTVT 63
           S++ R+S   +V L L   + +S     EL   V E   +Q+     V  S   + G  +
Sbjct: 6   SVIVRSSNFIVVILFLNLVTFFSLVSSNELVFAVGETGSLQVTPRTEVIYSPGLKRGITS 65

Query: 64  LYERVHIQGLQRFL-NLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQW 123
           + ER+HI GLQR L ++ + AH++ + +    S++ +++  VCFH+N+S  IGMCP +QW
Sbjct: 66  ICERLHIHGLQRRLEHIDRYAHSLKLTL---LSNNASNSIDVCFHRNSSRAIGMCPDNQW 125

Query: 124 EKAFEG-SWAQFMSPFDHRILDIRTSGLS--LETFEVSIEEEFSRYRIIFLILGVVLMSS 183
           ++  +G  W   MSPFD++ILDIRT G S  + T E+  ++EF  YRI+FLI+G+VL+S 
Sbjct: 126 KRVSKGLPWVGIMSPFDYKILDIRTFGGSSNVSTVELVAKQEFFIYRIVFLIMGIVLLSL 185

Query: 184 ASILSKLLVFYLGGGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFF 243
           AS LSK + FY  G   I  ++L+ +I++Q +K L  RGK+  Q+F   Y+S+  +G +F
Sbjct: 186 ASRLSKSVAFYYIGAMSIGIVILVTLIINQGIKRLPTRGKSRFQLFF--YSSMIGVGGYF 245

Query: 244 LHYVLDLLNQIVLEMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKFVDRENGLI 303
           L Y+  L+  ++++  I IS+D++ PLA+   L+  +  +G W GFW   KFV  ++G +
Sbjct: 246 LQYIRGLVQDLLMQ--IEISEDLYIPLAI--LLVVFVFMLGAWSGFWTVKKFVVTKDGSV 305

Query: 304 EKNISHFVVSTSIQILATFLILKCSLDPILATGGLICGTMASIMTSNIFKFQL--NFLQS 363
           + + S F VS SI+  A  LIL+ SLDP+LA G LI G + S++  +I +  L     + 
Sbjct: 306 DISTSIF-VSWSIRAFAVALILQSSLDPLLAGGALITGILISLILKSISRKLLAQRIYEQ 365

Query: 364 PNETSN------HLVEYRL---RTDLLQHRSSFTHDDDVYPSMFHSTHE-RRKISKDEWE 423
            +ET +      H   +     R    + R+    + D++PS FH T E RRK++K+E +
Sbjct: 366 DDETESCSPGLIHATSFASPLPRGSNKKLRTVPLSESDIFPSSFHKTPEGRRKLTKEELQ 425

Query: 424 RLTKDSTKKALEELVSSSGFTRWLLDNAGRINIPPLQ 444
           + TK+ST+ AL+ELVSS GF  W + NA RI + P++
Sbjct: 426 KFTKESTENALKELVSSPGFGEWAVKNAKRITVNPIK 452

BLAST of CSPI03G29900 vs. TAIR 10
Match: AT1G28760.1 (Uncharacterized conserved protein (DUF2215) )

HSP 1 Score: 147.9 bits (372), Expect = 2.0e-35
Identity = 134/483 (27.74%), Postives = 234/483 (48.45%), Query Frame = 0

Query: 13  LVFLSLFFASAYSTPDPEELRLLVSEPTIIQLFRYLPVENSSSSRLGTVTLYERVHIQGL 72
           +VFL L +A+A  +  P E  L +S    ++    + V   S ++LG      RV++  +
Sbjct: 21  IVFLLLSYAAALKSVGPGEPALNIS----LRSCERIQVSGYSRTKLGKFANSLRVNLVPI 80

Query: 73  QRFLNLKKVAHTVTVKVSMKSSSSRTSNFYVCFHKNTSLGIGMCPQSQWEKAFEGSWAQF 132
              L+LK                       VC H+N +LG+  C +S W+     S    
Sbjct: 81  PEKLHLK---------------------IQVCVHRNATLGMCHCEKSNWKNLQSSS---V 140

Query: 133 MSPFDHRILDIRTSGLSLET----FEVSIEEEFSRYRIIFLILGVVLMSSASILSKLLVF 192
           +SP+D + +D+R +G   ++      V++ EEF ++RI  L+ G++++  A ++S  L F
Sbjct: 141 ISPYDKQYIDVRFTGSEYQSSRSMITVNVTEEFHQWRIFCLVAGLIVILLAPVVSSWLPF 200

Query: 193 YLGGGWLIRFLLLLLMILSQRMKLLSRRGKNSLQIFLYAYASVGCLGSFFLHYVLDLLNQ 252
           Y      +   L++L+I+ Q M+LL    KN +  +L  Y SV   GSF LH    ++N 
Sbjct: 201 YYTSSMAVGVFLVVLIIIFQVMRLLPTGRKNVM--YLAFYGSVVGAGSFILHQFSMMVNM 260

Query: 253 IVLEMGITISQDMFDPLALATFLIAIILPIGTWLGFWVAHKF-VDRENGLIEKNISHFVV 312
           I++  G  +S+DM++P+A+   L+ +++  G   GFW   KF V ++ G ++ +++ FV 
Sbjct: 261 ILVNFG--LSEDMYNPVAILV-LVGVVI-TGAAFGFWTVRKFVVSKDGGGVDASVAQFVK 320

Query: 313 STSIQILATFLILKCSLDPILATGGLICGTMASIMTSNIFKF------------------ 372
                + ATF IL+ SLD  +A G  +  ++   + S    F                  
Sbjct: 321 WAMRSVAATF-ILQSSLDTPMAMGAFVLASLFGFLVSKTKSFVGKSHWLVSAGERRPIMH 380

Query: 373 -QLNFLQSPN-----ETSNHLVEYRLRTD---LLQHRSSFTH----DDDVYPSMFHSTHE 432
            +  FL  P      +++  +  Y        ++   SS        +  Y S FH+T  
Sbjct: 381 GRAEFLSRPGGGGLWKSAKSVPSYSGSPSYDVIVSSPSSVNRRIPIGNQDYYSTFHTTPN 440

Query: 433 RRKISKDEWERLTKDSTKKALEELVSSSGFTRWLLDNAGRINIPPLQGV---ELESVESD 457
           R+++SK E++ LT+++T++A+  L +S GF+ WL+++A RI + P       EL S ESD
Sbjct: 441 RKRMSKREYDELTQETTREAMAGLAASPGFSDWLVEHADRIKLLPTDSSYDDELGS-ESD 467

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0LD373.7e-25899.58Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G701570 PE=3 SV=1[more]
A0A1S3CFC02.4e-13259.61uncharacterized protein LOC103500297 OS=Cucumis melo OX=3656 GN=LOC103500297 PE=... [more]
A0A1S4E3G61.4e-12759.79nuclear envelope integral membrane protein 1 isoform X1 OS=Cucumis melo OX=3656 ... [more]
A0A6J1G8K05.7e-12658.35uncharacterized protein LOC111451852 OS=Cucurbita moschata OX=3662 GN=LOC1114518... [more]
A0A6J1L1F67.4e-12658.56uncharacterized protein LOC111499541 OS=Cucurbita maxima OX=3661 GN=LOC111499541... [more]
Match NameE-valueIdentityDescription
XP_011652833.28.9e-25999.79uncharacterized protein LOC105435121 [Cucumis sativus] >KAE8650939.1 hypothetica... [more]
XP_008461773.14.9e-13259.61PREDICTED: uncharacterized protein LOC103500297 [Cucumis melo][more]
XP_038906035.15.4e-13160.42uncharacterized protein LOC120091936 [Benincasa hispida][more]
XP_016902769.12.8e-12759.79PREDICTED: nuclear envelope integral membrane protein 1 isoform X1 [Cucumis melo... [more]
XP_011651793.18.1e-12759.37uncharacterized protein LOC101210632 isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT5G67610.13.0e-7139.02Uncharacterized conserved protein (DUF2215) [more]
AT5G67610.23.7e-6939.01Uncharacterized conserved protein (DUF2215) [more]
AT3G49840.12.2e-6637.42Uncharacterized conserved protein (DUF2215) [more]
AT1G28760.12.0e-3527.74Uncharacterized conserved protein (DUF2215) [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019358NEMP familyPFAMPF10225NEMPcoord: 154..429
e-value: 5.7E-22
score: 78.6
NoneNo IPR availablePANTHERPTHR31587:SF4TRANSMEMBRANE PROTEIN (DUF2215)coord: 11..443
NoneNo IPR availablePANTHERPTHR31587TRANSMEMBRANE PROTEIN (DUF2215)coord: 11..443

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G29900.1CSPI03G29900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005637 nuclear inner membrane