CSPI03G29820 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G29820
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCytochrome P450
LocationChr3: 27330098 .. 27334900 (-)
RNA-Seq ExpressionCSPI03G29820
SyntenyCSPI03G29820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAGTGCACTAAAAAGCTCAAGCAAACTCATGATTCACGTTGATCGCTGGTGACCAATTGGCACTATTTTCTTCACTAATATATTCTAATACTTCTTCCATACAATCATCTCATAATCATCTCCTTCTCAATTCAATCATATAAATATATAACCCCATCAAATTCACTTCACTCCCTTCTTCCTTCCACACACACCTAACAATAATGCCGGACGCCGCTCTTCACTACACTCTCTACCTTCTCCTCTTCTTCCTCTCCTCTCTCCTTCTCTTCTTCTTCCTCCACAAACCCTCCAAATCCGCCTCCGCAAATCTCACTCTACCTCCATCTCCACCGCCGCTCCCAGTCATCGGCCACCTCCATCTCCTCCTCCCTGCCACTCACAAATCCTTCTTCAACATCTCCTCTAAGTACGGTCCGCTCCTCCACATCCGCCTCGGCGCTCTGCAGTACGTCCTCGTCTCCTCCGCCTCCCTCGCTGCTGAGATCTTCAAAACGCACGATCTCACCTTCTCCTCCCGACCTGATTTCGCCTTCAGCGAGGAGTATCCGTACGGAAAAGTGGGATTCCTTGGCGCTCCGTACGGGGATTATTGGCGGTATATGAAGAAGCTGACGATGATGGAGGTTCTTGCGGCGCCACAGCTTGCACGATCGCGATTTGTGAGGAATGAAGAGATCCTTCGTATGCTTCAAAAGTTGCTGCTCTGTTCCAAGCAGAAGCAGGTACTCTCTTAACTAACTAACTCCTTTTCTGTTTTCTTCACTATTTCTTTATTAGATTTTTTTTGCAAGCTATGGTTTTTTCTTTTATTCTTAATTTCTATTAATTACTAATTAATTAAAATTAATTATGATATAAAAGTTTAAGTCACATTTTTACCACGATTTTAGACGCAATACAATTATTGATATACTTCTATAATTGTCTTTTATTAGGAATAATTTTTAAAATTTTACTGTATTTTATAAATATTTTTTTTAAAAATATAATGCCACACCCCACCCCGCCTCGAGCACCTACGTATTCAACCCGAAATGTGGCATGAAGCCAACCGTTCTTAACAACATCAATTGACCTTAACTACTATTCTTTTACGATGCTAAACAACTTAGAAAAAATTAAAACTTATGCAACGAAAACTACGAGAAACAACTTGAACAACTTCTTATACTTTAAACAGGTAGCCGAGGCTAAATGCCATTGATTACAAACTTTACTTACATATCAAACATAAATTATACAACAACACTTAACCTTAAGCAAAACCATGTACACGATAAGATTAAAACTGACCTTTAATATATAAACTATCAACAACTTCGCTCAGGAGTCTGAAAACAACGGTTAGGTTCTCGGATCACGACCTCTACCTGAAAAGTGGAAAAATATTTTAAAAGGATGAGCTAAAAAGCCTAGTAAGTGATGGTTTTATAAAATAGACTTTTAAAACAAACATACTTTAAACATTTCATAAACATACCATATAATTTTAAGAAAACTCTTTAAACTGAACACTTTCTAACATTTGAATATTCATTTAACTCTGTATACTCTATACCTAGAAAAACTTAAACAAGATAGCAAAGCACCTTCGATCATACTATCCAATTAAACCTCAATAGAATAGCAAAGCACGTTCGGTCATACTATTCATAATAAACTCGATCAAGATAGTAGAACACATTCGGTAATATTATTCCTGCATGAACTTGATGGTAGTGACTTAGTGGTCAGCATAGCCCTGACTATGCACATTGACCATCAAGAACTCGGTATATAAAGTACCATGGTTTTCTCTTTAAAACCATTATATTAAACTCATGTTTTTCATAAATCATTGCTTGTAAATCTTATAAATACATGCTTGCTAAAATCATTTATAAACTCATGCTTAGAACACTTTTCTAAATAACACGCTTTAACACTTTTTCATAAACATGGTGATAAACAAAAATAAGGCTCGCTTAAAAAGCATACCTAAATAATAACTTGTAAAACATATTAGAATTATTCTTTAGGAATCATTTTACTACTCATATATCATAAGTTGAAATAACTTTAGTAAACTCATTTTTCACTCACAGATCGCAGATCAAACCCTTCGTCGATATGCTAGTCCAAATATCTCTTGTCCTGAAATAATGGAAAAGAACTTCAATTAATACCCTTTTATTTTAAAACATCAAACTTACTTAAAGTTTCCTAAATCATAAAAACAACCTCAAAACATCTCGAAAAACCTCTCTTAATCCAAAACACATGTCCAACATGTCATGCTAATTTCTTAGGCTCGGCCACTAACCTCTACTGCCCAAAACTTATTATCGGCTAGAACTCTACTTCTTCTTTAGAGTCTTATAACTTAAGATTCAGAATTCAAATGAAAATATTTTCTTCTACAAAGTCGTTTCAAAATGTCTTAACTTTCTTACTGCAAACTTTTAGATCAAAACACCAAACTATGCGTTATATAGCCTTCTGGGAGTGCATCTCACTCATATTTACTCGCGAACTGACCTTCTGGCATTCTCTTATCCAAAACGCCAACTTTCTCATATCAAAAACGCCCAGCCGCCTTCCTTAATAAACTAGACTCATTTTTTGAACTTTCAAGACCAATTTGTCTGAAATTGCTCAAGCAAGTGAGTTGCGAGAAACTCTTTTTCGAAGTTAGCTAAAACGTCCAATCTCTATGATCCTTCTTTCTTGCCAACCTTAAACACTCAAAACGCCTAAAGTACTATTCCTTGATACTTAATAAAAAAAAAAATTCCTTCTTTCAAATCTCTTAAACTCTTTGAAAAATTTACACCGGGTCTTATGTTATGAAAAATTTATTAAAGGGAAGATTGAAAATTAACTATGTTAAGATATATATCAATTTATACCTTCAATTTTGGATGGTATAATTAAAATGAACACTCTCAAATATAGCAAAACAAAATTATTGTTGCATTGATAGAATTTTAAGTTTTTTTTTTTTTGTATTATTTTGTATATATTTTTCAATAATTTTTCTAATTAAAATTATCAACTAATAATTATATTAATTTAAATATCAAAGAAGAATTTGTATCAAACTTTTGTAATCATATCAATTTAATTTCATACTTCTTACATCGCCAATTGAACAAGAACGTAACACAATCGACCTTTAACGTTATATTATTTAAACACATGCTTGTTTTTTTTGTGGGATTGAATGAGTGTTATGTAAATGTTGATATGAGTGAATACTTTTGAAAAAATTGTATTAAAAAAATAAATTTAAATCACTAAATAAATATTGATTGAACAAACATGCATTTAATAAATAATTAAGAGGGAAAATAATGAATTAATGAATTTGTTGTTGAAATGGAAAACAGTCAGTGGACTTGGGGGCAGAGTTGATTAAGCTCACAAACAACAGCATCTGCAGAATGATGATGAGCACAAGATGTTCCGAAGAGAACAACGAAGCAGAGAAGATCAGAATTTTGGTTAACGACACCATTGAAATTGCTACGAAGATGGCTTTCGGAGATCTCTTCACCCAAGGTCCATTGAAGAGGCTTCCCTTCTGGCTCTTTGGCAACAAAGCTCTCCAAATCAATGTCCGATTCGACCTTCTCCTTGAAAACATTCTGCAACAACACGAACAACGAGCCAAAATCCACGGCCTCGAAAGAGAAGACTGCGATCTCATGGATATATTACTCAAAGCATATTTGGATGAGAAAGCCGAGTTCAAAATGACTAGAAATCACATCAAAGCCTTCTTACTTGTGAGTTCCATTTGTAAACCATTCTAATTCTTTGTTTCCTCTGTTCTAAATCCTAATATTATAAGACTCTCTTGTATTGACTGACCTTTCGATTGATCAAGTACCAACACCCTTACTTAGAAGCACCATTGTTTTGTGTTATGTAGCATTTTTTGTTTCGGGATTTCGTAACAACTAACATTTGATTTGTTCCTCTTAGGATCTGTTCATTGCCGGCACCGGAACGTCGGCTGAGGTTATGCAGTGGGCAATGGCGGAACTAATGAACCATCCGGATGTGTTCCAGAAGGTGCGGCGAGAGATCGAATCAGTCGCCGGGACGAGACTTGTGGAAGAAACGGATGTTACAAATCTTCCGTACACGCAGGCGGTAGTGAAGGAGTGTCTCCGGCTGTATCCAGCGGTGCCGGTGGCGAGACGAGCGTGCCGAGAAACTTGCAAGGTGAATGGATACGATATTCCAAAGGACATCATGGTTGCAGTGGACCTGTTTGCGATAATGCGGGATCCAAATCTGTGGGAGAATCCAGACGAATTTCGGCCTGAGAGATTCTACAATGAGAATTCCAGTAAAGAAGAAGGAACGAAACACATTCAATATGAGATTAAGGGACAGAGCTTCAGCTTTGTGCCATTCGGAGGAGGAAGAAGGGGATGCCCGGGGTCATTGTTAGCGTTCAACACGATCAATAGGACGGTGGCGGCATTGGTTCAGTGCTTTGATTGGAAGGTTGGGAAAGATGGAGATGAAGAGAAGGTGAATATGGAGATTGGAACAGGAATTAGTTTGCCAATGGCTCATCCATTGATCTGTGTGCCTGTTTCTCATTCAACTCCTTTTGTTGCTCAGTAAAGCCATCGACCTCATTGTGACAATCTGCCATGGGAGGTTTGGCCTTCTCTATTCTTAGACTTTTTCATCATGTGTTCTTAAGGTTTTTTTTTGGTTTGTACTGAACAAATGCATATTAATAACTATTAATAATGGAATCCAAGAATATCATGAATGTATAATCATACTCAGTGCATCA

mRNA sequence

GAGAGTGCACTAAAAAGCTCAAGCAAACTCATGATTCACGTTGATCGCTGGTGACCAATTGGCACTATTTTCTTCACTAATATATTCTAATACTTCTTCCATACAATCATCTCATAATCATCTCCTTCTCAATTCAATCATATAAATATATAACCCCATCAAATTCACTTCACTCCCTTCTTCCTTCCACACACACCTAACAATAATGCCGGACGCCGCTCTTCACTACACTCTCTACCTTCTCCTCTTCTTCCTCTCCTCTCTCCTTCTCTTCTTCTTCCTCCACAAACCCTCCAAATCCGCCTCCGCAAATCTCACTCTACCTCCATCTCCACCGCCGCTCCCAGTCATCGGCCACCTCCATCTCCTCCTCCCTGCCACTCACAAATCCTTCTTCAACATCTCCTCTAAGTACGGTCCGCTCCTCCACATCCGCCTCGGCGCTCTGCAGTACGTCCTCGTCTCCTCCGCCTCCCTCGCTGCTGAGATCTTCAAAACGCACGATCTCACCTTCTCCTCCCGACCTGATTTCGCCTTCAGCGAGGAGTATCCGTACGGAAAAGTGGGATTCCTTGGCGCTCCGTACGGGGATTATTGGCGGTATATGAAGAAGCTGACGATGATGGAGGTTCTTGCGGCGCCACAGCTTGCACGATCGCGATTTGTGAGGAATGAAGAGATCCTTCGTATGCTTCAAAAGTTGCTGCTCTGTTCCAAGCAGAAGCAGTCAGTGGACTTGGGGGCAGAGTTGATTAAGCTCACAAACAACAGCATCTGCAGAATGATGATGAGCACAAGATGTTCCGAAGAGAACAACGAAGCAGAGAAGATCAGAATTTTGGTTAACGACACCATTGAAATTGCTACGAAGATGGCTTTCGGAGATCTCTTCACCCAAGGTCCATTGAAGAGGCTTCCCTTCTGGCTCTTTGGCAACAAAGCTCTCCAAATCAATGTCCGATTCGACCTTCTCCTTGAAAACATTCTGCAACAACACGAACAACGAGCCAAAATCCACGGCCTCGAAAGAGAAGACTGCGATCTCATGGATATATTACTCAAAGCATATTTGGATGAGAAAGCCGAGTTCAAAATGACTAGAAATCACATCAAAGCCTTCTTACTTGATCTGTTCATTGCCGGCACCGGAACGTCGGCTGAGGTTATGCAGTGGGCAATGGCGGAACTAATGAACCATCCGGATGTGTTCCAGAAGGTGCGGCGAGAGATCGAATCAGTCGCCGGGACGAGACTTGTGGAAGAAACGGATGTTACAAATCTTCCGTACACGCAGGCGGTAGTGAAGGAGTGTCTCCGGCTGTATCCAGCGGTGCCGGTGGCGAGACGAGCGTGCCGAGAAACTTGCAAGGTGAATGGATACGATATTCCAAAGGACATCATGGTTGCAGTGGACCTGTTTGCGATAATGCGGGATCCAAATCTGTGGGAGAATCCAGACGAATTTCGGCCTGAGAGATTCTACAATGAGAATTCCAGTAAAGAAGAAGGAACGAAACACATTCAATATGAGATTAAGGGACAGAGCTTCAGCTTTGTGCCATTCGGAGGAGGAAGAAGGGGATGCCCGGGGTCATTGTTAGCGTTCAACACGATCAATAGGACGGTGGCGGCATTGGTTCAGTGCTTTGATTGGAAGGTTGGGAAAGATGGAGATGAAGAGAAGGTGAATATGGAGATTGGAACAGGAATTAGTTTGCCAATGGCTCATCCATTGATCTGTGTGCCTGTTTCTCATTCAACTCCTTTTGTTGCTCAGTAAAGCCATCGACCTCATTGTGACAATCTGCCATGGGAGGTTTGGCCTTCTCTATTCTTAGACTTTTTCATCATGTGTTCTTAAGGTTTTTTTTTGGTTTGTACTGAACAAATGCATATTAATAACTATTAATAATGGAATCCAAGAATATCATGAATGTATAATCATACTCAGTGCATCA

Coding sequence (CDS)

ATGCCGGACGCCGCTCTTCACTACACTCTCTACCTTCTCCTCTTCTTCCTCTCCTCTCTCCTTCTCTTCTTCTTCCTCCACAAACCCTCCAAATCCGCCTCCGCAAATCTCACTCTACCTCCATCTCCACCGCCGCTCCCAGTCATCGGCCACCTCCATCTCCTCCTCCCTGCCACTCACAAATCCTTCTTCAACATCTCCTCTAAGTACGGTCCGCTCCTCCACATCCGCCTCGGCGCTCTGCAGTACGTCCTCGTCTCCTCCGCCTCCCTCGCTGCTGAGATCTTCAAAACGCACGATCTCACCTTCTCCTCCCGACCTGATTTCGCCTTCAGCGAGGAGTATCCGTACGGAAAAGTGGGATTCCTTGGCGCTCCGTACGGGGATTATTGGCGGTATATGAAGAAGCTGACGATGATGGAGGTTCTTGCGGCGCCACAGCTTGCACGATCGCGATTTGTGAGGAATGAAGAGATCCTTCGTATGCTTCAAAAGTTGCTGCTCTGTTCCAAGCAGAAGCAGTCAGTGGACTTGGGGGCAGAGTTGATTAAGCTCACAAACAACAGCATCTGCAGAATGATGATGAGCACAAGATGTTCCGAAGAGAACAACGAAGCAGAGAAGATCAGAATTTTGGTTAACGACACCATTGAAATTGCTACGAAGATGGCTTTCGGAGATCTCTTCACCCAAGGTCCATTGAAGAGGCTTCCCTTCTGGCTCTTTGGCAACAAAGCTCTCCAAATCAATGTCCGATTCGACCTTCTCCTTGAAAACATTCTGCAACAACACGAACAACGAGCCAAAATCCACGGCCTCGAAAGAGAAGACTGCGATCTCATGGATATATTACTCAAAGCATATTTGGATGAGAAAGCCGAGTTCAAAATGACTAGAAATCACATCAAAGCCTTCTTACTTGATCTGTTCATTGCCGGCACCGGAACGTCGGCTGAGGTTATGCAGTGGGCAATGGCGGAACTAATGAACCATCCGGATGTGTTCCAGAAGGTGCGGCGAGAGATCGAATCAGTCGCCGGGACGAGACTTGTGGAAGAAACGGATGTTACAAATCTTCCGTACACGCAGGCGGTAGTGAAGGAGTGTCTCCGGCTGTATCCAGCGGTGCCGGTGGCGAGACGAGCGTGCCGAGAAACTTGCAAGGTGAATGGATACGATATTCCAAAGGACATCATGGTTGCAGTGGACCTGTTTGCGATAATGCGGGATCCAAATCTGTGGGAGAATCCAGACGAATTTCGGCCTGAGAGATTCTACAATGAGAATTCCAGTAAAGAAGAAGGAACGAAACACATTCAATATGAGATTAAGGGACAGAGCTTCAGCTTTGTGCCATTCGGAGGAGGAAGAAGGGGATGCCCGGGGTCATTGTTAGCGTTCAACACGATCAATAGGACGGTGGCGGCATTGGTTCAGTGCTTTGATTGGAAGGTTGGGAAAGATGGAGATGAAGAGAAGGTGAATATGGAGATTGGAACAGGAATTAGTTTGCCAATGGCTCATCCATTGATCTGTGTGCCTGTTTCTCATTCAACTCCTTTTGTTGCTCAGTAA

Protein sequence

MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATHKSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLPYTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQCFDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVAQ*
Homology
BLAST of CSPI03G29820 vs. ExPASy Swiss-Prot
Match: Q9LJY5 (Cytochrome P450 705A22 OS=Arabidopsis thaliana OX=3702 GN=CYP705A22 PE=2 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 1.3e-120
Identity = 229/514 (44.55%), Postives = 338/514 (65.76%), Query Frame = 0

Query: 10  LYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPA-THKSFFNISS 69
           +++L+F L+ L  FFF  KP K +  N  LPPSPP LP+IGH+HLLL   THKS   +SS
Sbjct: 14  IFILIFLLTFLCFFFFFKKP-KDSRVNFDLPPSPPSLPIIGHVHLLLSTLTHKSLQKLSS 73

Query: 70  KYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGFLGAPYG 129
           +YGPLL++R+  +  +LVSSAS+A EIF+T D+  SSR   A  E   +G   F+ APYG
Sbjct: 74  RYGPLLYLRIFNVPIILVSSASVAYEIFRTQDVNISSRGVTAVDESLVFGSSSFVTAPYG 133

Query: 130 DYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAELIKLTNN 189
           DYW++MKKLT+M++L      +SR +R ++I R  + LL  +++K+SV++G E + L NN
Sbjct: 134 DYWKFMKKLTVMKLLGPQAQEQSRDIRADDIKRFCRNLLDKARKKESVEIGKEAMNLMNN 193

Query: 190 SICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLFGNKALQ 249
            +C+M M    SEEN E EK+R LV ++I +  KM F  +  +  L++L   LF    + 
Sbjct: 194 ILCKMSMGRSFSEENGETEKLRGLVTESIGLMKKM-FLAVLLRRQLQKLGISLFKKDIMG 253

Query: 250 INVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHIKAFLLD 309
           ++ +FD+LLE +L +H ++ +    + +   ++D+LL AY DE AE+K+T+NHIKAF +D
Sbjct: 254 VSNKFDVLLEKVLVEHREKPE----KDQGTVMLDVLLAAYGDENAEYKITKNHIKAFFVD 313

Query: 310 LFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAG-TRLVEETDVTNLPYTQAVVK 369
           LFI  T TS + +QW MAE+MN+  + +++R EI+SV G +RL++ETD+ NLPY  AV+K
Sbjct: 314 LFIGATDTSVQTIQWTMAEIMNNTHILERMREEIDSVVGKSRLIQETDLPNLPYLHAVIK 373

Query: 370 ECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRPERFYN 429
           E LRL+P  P+  R  ++ CK+ G+ IP+   + ++ + +MRDPN+WE+P+EF+PERF  
Sbjct: 374 EALRLHPPGPLLPREFQQGCKIGGFYIPEKTTLLINAYVVMRDPNVWEDPEEFKPERFLA 433

Query: 430 ENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQCFDWKVGK 489
            + S +E       E + Q+  F+PFG GRRGCPGS LA+  +   +  +VQCFDW++  
Sbjct: 434 SSRSGQED------ERREQALKFLPFGSGRRGCPGSNLAYMIVGSAIGMMVQCFDWRI-- 493

Query: 490 DGDEEKVNM-EIGTGISLPMAHPLICVPVSHSTP 521
             + EKVNM E   G  L MAHPL   PV+   P
Sbjct: 494 --EGEKVNMKEAVKGTILTMAHPLKLTPVTRQPP 511

BLAST of CSPI03G29820 vs. ExPASy Swiss-Prot
Match: Q9FI39 (Cytochrome P450 705A5 OS=Arabidopsis thaliana OX=3702 GN=CYP705A5 PE=2 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.6e-118
Identity = 236/508 (46.46%), Postives = 328/508 (64.57%), Query Frame = 0

Query: 10  LYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLL-PATHKSFFNISS 69
           ++LLL   S L    F  K +K + A   LPPSPP LP+IGHLHL+L    H+SF NISS
Sbjct: 14  IFLLLCLFSRLSYDLFFRK-TKDSRAGCALPPSPPSLPIIGHLHLILFVPIHQSFKNISS 73

Query: 70  KYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGFLGAPYG 129
           KYGPLLH+R      VLVSSAS A EIFK  D+  SSRP     E    G   F+  PYG
Sbjct: 74  KYGPLLHLRFFNFPIVLVSSASTAYEIFKAQDVNVSSRPPPPIEESLILGSSSFINTPYG 133

Query: 130 DYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAELIKLTNN 189
           DY ++MKK  + ++L    L RSR +R +E+ R  + LL  + +KQ+V++  E +KLTNN
Sbjct: 134 DYSKFMKKFMVQKLLGPQALQRSRNIRADELERFYKTLLDKAMKKQTVEIRNEAMKLTNN 193

Query: 190 SICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLFGNKALQ 249
           +IC+M+M   CSEEN EAE +R LV ++I +  K   G +F + PLK+L   LF  + + 
Sbjct: 194 TICKMIMGRSCSEENGEAETVRGLVTESIFLTKKHFLGAMFHK-PLKKLGISLFAKELMN 253

Query: 250 INVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHIKAFLLD 309
           ++ RFD LLE IL +HE++ + H    +  D++D+LL+AY DE AE+K+TR+ IK+  +D
Sbjct: 254 VSNRFDELLEKILVEHEEKLQEH---HQTSDMLDMLLEAYGDENAEYKITRDQIKSLFVD 313

Query: 310 LFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAG-TRLVEETDVTNLPYTQAVVK 369
           LF AGT  SA  +QW MAE++ +P + +++R EI+SV G TRLV+ETD+ NLPY QA+VK
Sbjct: 314 LFSAGTEASANTIQWTMAEIIKNPKICERLREEIDSVVGKTRLVQETDLPNLPYLQAIVK 373

Query: 370 ECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRPERFYN 429
           E LRL+P  PV  R  +ETC++ G+ IP+   + V+++AIMRDP+ WE+P+EF+PERF  
Sbjct: 374 EGLRLHPPGPVV-RTFKETCEIKGFYIPEKTRLFVNVYAIMRDPDFWEDPEEFKPERFLA 433

Query: 430 ENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQCFDWKVGK 489
            +   EE  K      +     ++PFG GRR CPGS LA+  +   +  +VQ FDW +  
Sbjct: 434 SSRLGEEDEK------REDMLKYIPFGSGRRACPGSHLAYTVVGSVIGMMVQHFDWII-- 493

Query: 490 DGDEEKVNMEIGTGISLPMAHPLICVPV 516
               EK+NM+ G  ++L MAHPL C PV
Sbjct: 494 --KGEKINMKEGGTMTLTMAHPLKCTPV 505

BLAST of CSPI03G29820 vs. ExPASy Swiss-Prot
Match: O81973 (Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 1.2e-110
Identity = 217/489 (44.38%), Postives = 304/489 (62.17%), Query Frame = 0

Query: 39  LPPSPPPLPVIGHLHLLLPATHKSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKT 98
           LPPSP PLP+IGHLHLL P  H+ F  +S +YGP++H+ LG++  V+ S+A  A E  KT
Sbjct: 31  LPPSPMPLPIIGHLHLLSPTPHQDFHKLSLRYGPIIHLFLGSVPCVVASTAEAAKEFLKT 90

Query: 99  HDLTFSSRP-DFAFSEEYPYGKVGFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNE 158
           H+  FS+RP +    E   YG   FL APYG YW++MKKL M E+L    L +   VR  
Sbjct: 91  HEPAFSNRPANTVAVETLTYGFQDFLFAPYGPYWKFMKKLCMSELLGGHMLDQFLPVRQX 150

Query: 159 EILRMLQKLLLCSKQKQSVDLGAELIKLTNNSICRMMMS-TRCSEENNEAEKIRILVNDT 218
           E  + ++++L      ++VD G E I L+NN + RM++S T  +E+ NE E++R LV D 
Sbjct: 151 ETKKFIKRVLQKGISGEAVDFGGEFITLSNNIVSRMIVSQTSTTEDENEVEEMRKLVKDA 210

Query: 219 IEIATKMAFGDLFTQGPLKRLPFWLFGNKALQINVRFDLLLENILQQHEQRAKIHGL--- 278
            E++ K    D  +   LKR     F  +  +I   FD +L+ I++Q E+  +       
Sbjct: 211 AELSGKFNISDFVSF--LKRFDLQGFNKRLEKIRDCFDTVLDRIIKQREEERRNKNETVG 270

Query: 279 EREDCDLMDILLKAYLDEKAEFKMTRNHIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPD 338
           +RE  D++D+L     DE +E K+ + +IKAF+LD+ IAGT TSA  M+WAMAEL+N+P 
Sbjct: 271 KREFKDMLDVLFDISEDESSEIKLNKENIKAFILDILIAGTDTSAVTMEWAMAELINNPG 330

Query: 339 VFQKVRREIESVAG-TRLVEETDVTNLPYTQAVVKECLRLYPAVPVARRACRETCKVNGY 398
           V +K R+E+++V G +R+VEE+D+ NLPY Q +V+E LRL+PA P+  R       V GY
Sbjct: 331 VLEKARQEMDAVVGKSRIVEESDIANLPYLQGIVRETLRLHPAGPLLFRESSRRAVVCGY 390

Query: 399 DIPKDIMVAVDLFAIMRDPNLWENPDEFRPERFYNENSSKEEGTKHIQYEIKGQSFSFVP 458
           DIP    + V+++AI RDPN WENP EFRPERF     S        Q +++GQ +  +P
Sbjct: 391 DIPAKTRLFVNVWAIGRDPNHWENPLEFRPERFVENGKS--------QLDVRGQHYHLLP 450

Query: 459 FGGGRRGCPGSLLAFNTINRTVAALVQCFDWKVGKDGDEEKVNMEIGTGISLPMAHPLIC 518
           FG GRR CPG+ LA   ++  +A L+QCF WKV  D D  KVNME   GI+LP AHP+IC
Sbjct: 451 FGSGRRACPGTSLALQVVHVNLAVLIQCFQWKV--DCDNGKVNMEEKAGITLPRAHPIIC 507

Query: 519 VPVSHSTPF 522
           VP+    PF
Sbjct: 511 VPIRRLNPF 507

BLAST of CSPI03G29820 vs. ExPASy Swiss-Prot
Match: Q9LJY7 (Cytochrome P450 705A20 OS=Arabidopsis thaliana OX=3702 GN=CYP705A20 PE=2 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 6.1e-110
Identity = 228/518 (44.02%), Postives = 323/518 (62.36%), Query Frame = 0

Query: 8   YTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPA-THKSFFNI 67
           ++  LL FF  SLL +  L K  K +     L  SPP LP+IGHLH LL +  HKS   +
Sbjct: 10  FSFILLCFF--SLLCYSLLFKKLKDSHVGRDLLQSPPSLPIIGHLHHLLSSLAHKSLQQL 69

Query: 68  SSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGFLGAP 127
           SSKYGPLLH+ +     VLVSSAS+A EIFK HDL  SSR +   +E    G   F+GAP
Sbjct: 70  SSKYGPLLHLSIFNFPVVLVSSASVAYEIFKAHDLNISSRDNPPINESLLVGSSVFVGAP 129

Query: 128 YGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAELIKLT 187
           YGDYW++MKKL + ++L    L RSR +R +E+ R  + LL  + +K+SV++G E  KL+
Sbjct: 130 YGDYWKFMKKLLVTKLLGPQALERSRSIRADELERFYRSLLDKAMKKESVEIGKEATKLS 189

Query: 188 NNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLFGNKA 247
            NSICRM M    SEE+ EAE++R LV +   +  K+   ++  + PL++L   LF  + 
Sbjct: 190 INSICRMSMGRSFSEESGEAERVRGLVTELDGLTKKVLLVNIL-RWPLEKLRISLFKKEI 249

Query: 248 LQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHIKAFL 307
           + ++  FD LLE I+ + E++      E +   LMD+LL+AY DEKAE K+TRNHIK+  
Sbjct: 250 MYVSNSFDELLERIIVEREKKPN----EHQGTYLMDVLLEAYEDEKAEHKITRNHIKSLF 309

Query: 308 LDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAG-TRLVEETDVTNLPYTQAV 367
           ++L + GT TSA+ +QW MAEL+N+ +V +++R EI+SV G TRL++E D+  LPY Q+V
Sbjct: 310 VELLLGGTDTSAQTIQWTMAELINNRNVLKRLREEIDSVVGETRLIQEKDLPKLPYLQSV 369

Query: 368 VKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRPERF 427
           VKE LRL+P +P+  R  + +C++ G+ I +   + V+ +A+MRDP  WE+PDEF+PERF
Sbjct: 370 VKEGLRLHPPLPLMVRTFQRSCEMKGFYIAEKTTLVVNAYAVMRDPTTWEDPDEFKPERF 429

Query: 428 YNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQCFDWKV 487
             +   +    KHI             FG GRRGCPGS LA   I   +  +VQCFD  +
Sbjct: 430 LRQEEER-RALKHI------------AFGSGRRGCPGSNLATIFIGTAIGTMVQCFDLSI 489

Query: 488 GKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVA 524
             D    KV M+   G++L MAHPL C+ V  + PF++
Sbjct: 490 KGD----KVKMDEVGGLNLTMAHPLECILVPRTQPFIS 503

BLAST of CSPI03G29820 vs. ExPASy Swiss-Prot
Match: Q9FH67 (Cytochrome P450 705A12 OS=Arabidopsis thaliana OX=3702 GN=CYP705A12 PE=3 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 2.8e-107
Identity = 221/517 (42.75%), Postives = 321/517 (62.09%), Query Frame = 0

Query: 9   TLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPAT--HKSFFNI 68
           ++++LL   S L    F  KP         LPPSPP LP+IGHLH LL ++  HKSF  +
Sbjct: 13  SIFILLCLFSFLCYALFFKKPK-----GFDLPPSPPSLPIIGHLHHLLSSSLPHKSFQKL 72

Query: 69  SSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGFLGAP 128
           S KYGPLLH+R+     VLVSSAS+A E+F+T+D+  S R      +   +G  GF+ AP
Sbjct: 73  SFKYGPLLHLRIFNFPMVLVSSASMAYEVFRTNDVNVSYRFVPVNKDSLVFGSSGFVTAP 132

Query: 129 YGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAELIKLT 188
           YGDYW++MKKL   ++L    L  S+  R EE+ R    L   +++K+SV++G   +KLT
Sbjct: 133 YGDYWKFMKKLISTKLLRPHALELSKGNRAEELRRFCLDLQGKARKKESVEIGKVALKLT 192

Query: 189 NNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLFGNKA 248
           NN ICRM M   CSE+N  AE+ R LVN +  ++ K+ F ++F +               
Sbjct: 193 NNIICRMSMGRSCSEKNGVAERARELVNKSFALSVKLFFSNMFRK-------------DI 252

Query: 249 LQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHIKAFL 308
           + ++  FD  LE IL +HE+  +      +D D++D LL+AY +E+AE+K+TR  IK+ +
Sbjct: 253 MGVSREFDEFLERILVEHEENLE----GDQDRDMIDHLLEAYRNEEAEYKITRKQIKSLI 312

Query: 309 LDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIES-VAGTRLVEETDVTNLPYTQAV 368
           +++F+ GT +SA+ +QW MAE++N+P V +K+R EI+S V G RL++E+D+ NLPY QAV
Sbjct: 313 VEIFLGGTDSSAQTIQWTMAEILNNPGVLEKLRAEIDSVVGGKRLIQESDLPNLPYLQAV 372

Query: 369 VKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRPERF 428
           VKE LRL+P+ PV  R   E+C+V  + +P+   + V+L+A+ RDP+ WE+PD F+PERF
Sbjct: 373 VKEGLRLHPSAPVLLRVFGESCEVKEFYVPEKTTLVVNLYAVNRDPDSWEDPDMFKPERF 432

Query: 429 YNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQCFDWKV 488
              + S +E       +I+ Q+  +V FGGGRR CP   LA   +   + A+VQCFDW++
Sbjct: 433 LVSSISGDE------EKIREQAVKYVTFGGGRRTCPAVKLAHIFMETAIGAMVQCFDWRI 492

Query: 489 GKDGDEEKVNMEIG-TGISLPMAHPLICVPVSHSTPF 522
                 EKV ME   +G+SL MAHPL C PV    PF
Sbjct: 493 ----KGEKVYMEEAVSGLSLKMAHPLKCTPVVRFDPF 497

BLAST of CSPI03G29820 vs. ExPASy TrEMBL
Match: A0A0A0L9V6 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa3G698490 PE=2 SV=1)

HSP 1 Score: 1043.1 bits (2696), Expect = 3.9e-301
Identity = 522/524 (99.62%), Postives = 523/524 (99.81%), Query Frame = 0

Query: 1   MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH 60
           MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH
Sbjct: 1   MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH 60

Query: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120
           KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV
Sbjct: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120

Query: 121 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 180
           GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA
Sbjct: 121 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 180

Query: 181 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFW 240
           ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFT+GPLKRLPFW
Sbjct: 181 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTRGPLKRLPFW 240

Query: 241 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRN 300
           LFGNKALQINVRFDLLLENILQQHEQRAKIHGLERED DLMDILLKAYLDEKAEFKMTRN
Sbjct: 241 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDRDLMDILLKAYLDEKAEFKMTRN 300

Query: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 360
           HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP
Sbjct: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 360

Query: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 420
           YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF
Sbjct: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 420

Query: 421 RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480
           RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC
Sbjct: 421 RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480

Query: 481 FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVAQ 525
           FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVAQ
Sbjct: 481 FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVAQ 524

BLAST of CSPI03G29820 vs. ExPASy TrEMBL
Match: A0A5D3CH28 (Cytochrome P450 705A5-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25G00320 PE=3 SV=1)

HSP 1 Score: 1016.1 bits (2626), Expect = 5.1e-293
Identity = 506/523 (96.75%), Postives = 512/523 (97.90%), Query Frame = 0

Query: 1   MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH 60
           MPDAALHY LYLLLFF SSLLLFFFLHKPSKSA  NLTLPPSPPPLPVIGHLHLLLPATH
Sbjct: 1   MPDAALHYALYLLLFFFSSLLLFFFLHKPSKSAPPNLTLPPSPPPLPVIGHLHLLLPATH 60

Query: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120
           KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV
Sbjct: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120

Query: 121 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 180
           GFLGAPYGDYWRYMKKLTMMEVLA PQLARSRFVRNEEILRMLQKLLLCSKQK+SVDLGA
Sbjct: 121 GFLGAPYGDYWRYMKKLTMMEVLAPPQLARSRFVRNEEILRMLQKLLLCSKQKESVDLGA 180

Query: 181 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFW 240
           EL+KLTNNSICRMMMSTRCSEENNEAEKIR LVNDTIEIATKMAFGDLF QGPLKRLPFW
Sbjct: 181 ELVKLTNNSICRMMMSTRCSEENNEAEKIRTLVNDTIEIATKMAFGDLFGQGPLKRLPFW 240

Query: 241 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRN 300
           LFG KAL+INVRFD+LLENILQQHEQRAKIHGLERED DLMDILLKAYLDEKAEFKMTRN
Sbjct: 241 LFGKKALEINVRFDVLLENILQQHEQRAKIHGLEREDRDLMDILLKAYLDEKAEFKMTRN 300

Query: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 360
           HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESV GTRLVEETDVTNLP
Sbjct: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVVGTRLVEETDVTNLP 360

Query: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 420
           YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKD MVAVDLFAIMRDPNLWENPDEF
Sbjct: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDTMVAVDLFAIMRDPNLWENPDEF 420

Query: 421 RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480
           RPERFYNENSSKEEGTKHIQYEIKGQ+FSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC
Sbjct: 421 RPERFYNENSSKEEGTKHIQYEIKGQNFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480

Query: 481 FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVA 524
           FDWKVGKDGDEEKVNMEIG+GISLPMAHPLICVPVSHSTPFVA
Sbjct: 481 FDWKVGKDGDEEKVNMEIGSGISLPMAHPLICVPVSHSTPFVA 523

BLAST of CSPI03G29820 vs. ExPASy TrEMBL
Match: A0A1S3CGU3 (cytochrome P450 705A5-like OS=Cucumis melo OX=3656 GN=LOC103500305 PE=3 SV=1)

HSP 1 Score: 1016.1 bits (2626), Expect = 5.1e-293
Identity = 506/523 (96.75%), Postives = 512/523 (97.90%), Query Frame = 0

Query: 1   MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH 60
           MPDAALHY LYLLLFF SSLLLFFFLHKPSKSA  NLTLPPSPPPLPVIGHLHLLLPATH
Sbjct: 1   MPDAALHYALYLLLFFFSSLLLFFFLHKPSKSAPPNLTLPPSPPPLPVIGHLHLLLPATH 60

Query: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120
           KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV
Sbjct: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120

Query: 121 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 180
           GFLGAPYGDYWRYMKKLTMMEVLA PQLARSRFVRNEEILRMLQKLLLCSKQK+SVDLGA
Sbjct: 121 GFLGAPYGDYWRYMKKLTMMEVLAPPQLARSRFVRNEEILRMLQKLLLCSKQKESVDLGA 180

Query: 181 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFW 240
           EL+KLTNNSICRMMMSTRCSEENNEAEKIR LVNDTIEIATKMAFGDLF QGPLKRLPFW
Sbjct: 181 ELVKLTNNSICRMMMSTRCSEENNEAEKIRTLVNDTIEIATKMAFGDLFGQGPLKRLPFW 240

Query: 241 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRN 300
           LFG KAL+INVRFD+LLENILQQHEQRAKIHGLERED DLMDILLKAYLDEKAEFKMTRN
Sbjct: 241 LFGKKALEINVRFDVLLENILQQHEQRAKIHGLEREDRDLMDILLKAYLDEKAEFKMTRN 300

Query: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 360
           HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESV GTRLVEETDVTNLP
Sbjct: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVVGTRLVEETDVTNLP 360

Query: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 420
           YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKD MVAVDLFAIMRDPNLWENPDEF
Sbjct: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDTMVAVDLFAIMRDPNLWENPDEF 420

Query: 421 RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480
           RPERFYNENSSKEEGTKHIQYEIKGQ+FSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC
Sbjct: 421 RPERFYNENSSKEEGTKHIQYEIKGQNFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480

Query: 481 FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVA 524
           FDWKVGKDGDEEKVNMEIG+GISLPMAHPLICVPVSHSTPFVA
Sbjct: 481 FDWKVGKDGDEEKVNMEIGSGISLPMAHPLICVPVSHSTPFVA 523

BLAST of CSPI03G29820 vs. ExPASy TrEMBL
Match: A0A6J1GBR6 (cytochrome P450 705A22-like OS=Cucurbita moschata OX=3662 GN=LOC111452721 PE=3 SV=1)

HSP 1 Score: 905.2 bits (2338), Expect = 1.3e-259
Identity = 453/526 (86.12%), Postives = 484/526 (92.02%), Query Frame = 0

Query: 8   YTLYLLLFFLSSLLLFFFLHKPSKSAS-------ANLTLPPSPPPLPVIGHLHLLLPATH 67
           Y LY LLFFLS L L FFLHK S SAS       A LTLPPSPP LP+IGHLHLLLPA +
Sbjct: 4   YILYFLLFFLSCLFLLFFLHKTSGSASSSSSAAAAKLTLPPSPPSLPLIGHLHLLLPANY 63

Query: 68  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 127
           KSFFNISSKYGPLLHIRL +LQYVLVSSASLAAEIF+THDLTFSSRPDFAFSEEYPYGKV
Sbjct: 64  KSFFNISSKYGPLLHIRLCSLQYVLVSSASLAAEIFRTHDLTFSSRPDFAFSEEYPYGKV 123

Query: 128 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 187
           GFLGAPYGDYWRYMKKLTMMEVLA PQLARSRFVRNEEI RML KLLL S++K+SVDLGA
Sbjct: 124 GFLGAPYGDYWRYMKKLTMMEVLAPPQLARSRFVRNEEISRMLHKLLLSSQKKESVDLGA 183

Query: 188 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFW 247
           EL+KLTNNSICRMMMSTRCSEENNEAE IR LVNDTIEIATK+AFGDLF++GPLKRLPFW
Sbjct: 184 ELVKLTNNSICRMMMSTRCSEENNEAENIRKLVNDTIEIATKLAFGDLFSEGPLKRLPFW 243

Query: 248 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRN 307
           LFG K L++N+RFD+LLE ILQQHE+RAKIHG ER+D DLMDILLKAYLDEKAEFK+TRN
Sbjct: 244 LFGKKVLKLNIRFDVLLEKILQQHEERAKIHGFERDDRDLMDILLKAYLDEKAEFKITRN 303

Query: 308 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 367
           HIKAFLLDLFIAGTGTSAEVMQW +AEL+NHP+VF+KVR+EIE V G+RLVEETD+ NLP
Sbjct: 304 HIKAFLLDLFIAGTGTSAEVMQWTIAELINHPNVFRKVRQEIEYVVGSRLVEETDIVNLP 363

Query: 368 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 427
           Y QAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF
Sbjct: 364 YMQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 423

Query: 428 RPERFYNENSSKEE---GTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAAL 487
           RPERFYN N++K+E   G KHIQYEIKGQ+FSFVPFGGGRRGCPGSLLA+NTINRTVAAL
Sbjct: 424 RPERFYNANTNKDEGDQGMKHIQYEIKGQNFSFVPFGGGRRGCPGSLLAYNTINRTVAAL 483

Query: 488 VQCFDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVA 524
           VQCFDWKVGKDG+ EKVNMEIGTGISLPMAH  ICVPVSHSTPFVA
Sbjct: 484 VQCFDWKVGKDGNGEKVNMEIGTGISLPMAHAFICVPVSHSTPFVA 529

BLAST of CSPI03G29820 vs. ExPASy TrEMBL
Match: A0A6J1KA72 (cytochrome P450 705A22-like OS=Cucurbita maxima OX=3661 GN=LOC111493061 PE=3 SV=1)

HSP 1 Score: 903.7 bits (2334), Expect = 3.7e-259
Identity = 453/524 (86.45%), Postives = 482/524 (91.98%), Query Frame = 0

Query: 8   YTLYLLLFFLSSLLLFFFLHKPSKSAS-----ANLTLPPSPPPLPVIGHLHLLLPATHKS 67
           Y LY LLFFLS L L F LHK S SAS     A LTLPPSPP LP+IGHLHLLLPA +KS
Sbjct: 4   YILYFLLFFLSCLFLLFLLHKTSGSASSSAAAAKLTLPPSPPSLPLIGHLHLLLPANYKS 63

Query: 68  FFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGF 127
           FFNISSKYGPLLHIRL +LQYVLVSSASLAAEIF+THDLTFSSRPDFAFSEEYPYGKVGF
Sbjct: 64  FFNISSKYGPLLHIRLCSLQYVLVSSASLAAEIFRTHDLTFSSRPDFAFSEEYPYGKVGF 123

Query: 128 LGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAEL 187
           LGAPYGDYWRYMKKLTMMEVLA PQLARSRFVRNEEI RML KLLL S++K+SVDLGAEL
Sbjct: 124 LGAPYGDYWRYMKKLTMMEVLAPPQLARSRFVRNEEISRMLHKLLLSSQKKESVDLGAEL 183

Query: 188 IKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLF 247
           IKLTNNSICRMMMSTRCSEENNEAE IR LVNDTIEIATK+AFGDLF++GPLKRLPFWLF
Sbjct: 184 IKLTNNSICRMMMSTRCSEENNEAENIRKLVNDTIEIATKLAFGDLFSEGPLKRLPFWLF 243

Query: 248 GNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHI 307
           G K L++N+RFD+LLE ILQQHE+RAKIHG ER+D DLMDILLKAYLDEKAEFK+TRNHI
Sbjct: 244 GKKVLKLNIRFDVLLEKILQQHEERAKIHGFERDDRDLMDILLKAYLDEKAEFKITRNHI 303

Query: 308 KAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLPYT 367
           KAFLLDLFIAGTGTSAEVMQW +AEL+NHP+VF+KVR+EIE V G+RLVEETD+ NLPY 
Sbjct: 304 KAFLLDLFIAGTGTSAEVMQWTIAELINHPNVFRKVRQEIECVVGSRLVEETDIVNLPYM 363

Query: 368 QAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRP 427
           QAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRP
Sbjct: 364 QAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRP 423

Query: 428 ERFYNENSSKEE---GTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQ 487
           ERFYN N +K+E   G KHIQYEIKGQ+FSFVPFGGGRRGCPGSLLA+NTINRTVAALVQ
Sbjct: 424 ERFYNANPNKDEGDQGMKHIQYEIKGQNFSFVPFGGGRRGCPGSLLAYNTINRTVAALVQ 483

Query: 488 CFDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVA 524
           CFDWKVGKDG+ EKVNMEIGTGISLPMAH  ICVPVSHSTPFVA
Sbjct: 484 CFDWKVGKDGNGEKVNMEIGTGISLPMAHSFICVPVSHSTPFVA 527

BLAST of CSPI03G29820 vs. NCBI nr
Match: NP_001292616.1 (cytochrome P450 705A5-like [Cucumis sativus] >AIT72034.1 cytochrome P450 [Cucumis sativus] >KGN58608.1 hypothetical protein Csa_002418 [Cucumis sativus])

HSP 1 Score: 1043.1 bits (2696), Expect = 8.0e-301
Identity = 522/524 (99.62%), Postives = 523/524 (99.81%), Query Frame = 0

Query: 1   MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH 60
           MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH
Sbjct: 1   MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH 60

Query: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120
           KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV
Sbjct: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120

Query: 121 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 180
           GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA
Sbjct: 121 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 180

Query: 181 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFW 240
           ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFT+GPLKRLPFW
Sbjct: 181 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTRGPLKRLPFW 240

Query: 241 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRN 300
           LFGNKALQINVRFDLLLENILQQHEQRAKIHGLERED DLMDILLKAYLDEKAEFKMTRN
Sbjct: 241 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDRDLMDILLKAYLDEKAEFKMTRN 300

Query: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 360
           HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP
Sbjct: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 360

Query: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 420
           YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF
Sbjct: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 420

Query: 421 RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480
           RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC
Sbjct: 421 RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480

Query: 481 FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVAQ 525
           FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVAQ
Sbjct: 481 FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVAQ 524

BLAST of CSPI03G29820 vs. NCBI nr
Match: XP_008461786.1 (PREDICTED: cytochrome P450 705A5-like [Cucumis melo] >KAA0048327.1 cytochrome P450 705A5-like protein [Cucumis melo var. makuwa] >TYK10670.1 cytochrome P450 705A5-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1016.1 bits (2626), Expect = 1.1e-292
Identity = 506/523 (96.75%), Postives = 512/523 (97.90%), Query Frame = 0

Query: 1   MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH 60
           MPDAALHY LYLLLFF SSLLLFFFLHKPSKSA  NLTLPPSPPPLPVIGHLHLLLPATH
Sbjct: 1   MPDAALHYALYLLLFFFSSLLLFFFLHKPSKSAPPNLTLPPSPPPLPVIGHLHLLLPATH 60

Query: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120
           KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV
Sbjct: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120

Query: 121 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 180
           GFLGAPYGDYWRYMKKLTMMEVLA PQLARSRFVRNEEILRMLQKLLLCSKQK+SVDLGA
Sbjct: 121 GFLGAPYGDYWRYMKKLTMMEVLAPPQLARSRFVRNEEILRMLQKLLLCSKQKESVDLGA 180

Query: 181 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFW 240
           EL+KLTNNSICRMMMSTRCSEENNEAEKIR LVNDTIEIATKMAFGDLF QGPLKRLPFW
Sbjct: 181 ELVKLTNNSICRMMMSTRCSEENNEAEKIRTLVNDTIEIATKMAFGDLFGQGPLKRLPFW 240

Query: 241 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRN 300
           LFG KAL+INVRFD+LLENILQQHEQRAKIHGLERED DLMDILLKAYLDEKAEFKMTRN
Sbjct: 241 LFGKKALEINVRFDVLLENILQQHEQRAKIHGLEREDRDLMDILLKAYLDEKAEFKMTRN 300

Query: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 360
           HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESV GTRLVEETDVTNLP
Sbjct: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVVGTRLVEETDVTNLP 360

Query: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 420
           YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKD MVAVDLFAIMRDPNLWENPDEF
Sbjct: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDTMVAVDLFAIMRDPNLWENPDEF 420

Query: 421 RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480
           RPERFYNENSSKEEGTKHIQYEIKGQ+FSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC
Sbjct: 421 RPERFYNENSSKEEGTKHIQYEIKGQNFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480

Query: 481 FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVA 524
           FDWKVGKDGDEEKVNMEIG+GISLPMAHPLICVPVSHSTPFVA
Sbjct: 481 FDWKVGKDGDEEKVNMEIGSGISLPMAHPLICVPVSHSTPFVA 523

BLAST of CSPI03G29820 vs. NCBI nr
Match: XP_038903604.1 (cytochrome P450 705A22-like [Benincasa hispida])

HSP 1 Score: 943.3 bits (2437), Expect = 8.7e-271
Identity = 468/527 (88.80%), Postives = 494/527 (93.74%), Query Frame = 0

Query: 1   MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH 60
           M DA+LHY LYLLLFFLS L LFFFLHKPSKSA   +TLP SPP LPVIGHLHLLLPA H
Sbjct: 1   MADASLHYALYLLLFFLSFLFLFFFLHKPSKSAPGKITLPSSPPSLPVIGHLHLLLPANH 60

Query: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120
           KSF NISSKYGPLLHIRLGALQYVLVSSASLAA+IFKT DL FSSRPDFAFSEEYPYGKV
Sbjct: 61  KSFINISSKYGPLLHIRLGALQYVLVSSASLAADIFKTQDLAFSSRPDFAFSEEYPYGKV 120

Query: 121 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 180
           GFLGAPYGDYWRYMKKLTMME+L+ PQLARSRFVR+EEI RML+ LLLCSK+K+SVDLGA
Sbjct: 121 GFLGAPYGDYWRYMKKLTMMELLSPPQLARSRFVRDEEITRMLRDLLLCSKKKESVDLGA 180

Query: 181 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFW 240
            LIKLTNNSICRMMMSTRCSE+NNEAEKIRILVNDTIEIATK+AFGDLF+ GPLKRLPFW
Sbjct: 181 MLIKLTNNSICRMMMSTRCSEQNNEAEKIRILVNDTIEIATKLAFGDLFSHGPLKRLPFW 240

Query: 241 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRN 300
           LFG K L IN++FD+LLENILQQHEQRAKIHGL+R+D DLMDILLKAYLDEKAEFKMTRN
Sbjct: 241 LFGKKTLDINLKFDVLLENILQQHEQRAKIHGLDRDDRDLMDILLKAYLDEKAEFKMTRN 300

Query: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 360
           HIKAFLLDLFIAGTGTSAEVMQW MAEL+NHPDVFQKVRREIESV GTRLVEETD+TNLP
Sbjct: 301 HIKAFLLDLFIAGTGTSAEVMQWTMAELINHPDVFQKVRREIESVIGTRLVEETDITNLP 360

Query: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 420
           Y QAVVKECLRLYPAVPVARRACRETCKVNG+DIPK IMVAVDLFAIM DPNLWENPDEF
Sbjct: 361 YMQAVVKECLRLYPAVPVARRACRETCKVNGFDIPKGIMVAVDLFAIMHDPNLWENPDEF 420

Query: 421 RPERFYNENSSKEE----GTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAA 480
           RPERFYN NS K+E    GT+HIQYEIKGQ+FSFVPFGGGRRGCPGSLLA+NTINRTVAA
Sbjct: 421 RPERFYNTNSGKDEGDHQGTRHIQYEIKGQNFSFVPFGGGRRGCPGSLLAYNTINRTVAA 480

Query: 481 LVQCFDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVA 524
           LVQCFDWKVGKDG+EE+VNMEIGTGISLPMAHPLICVPVSHSTPFVA
Sbjct: 481 LVQCFDWKVGKDGNEERVNMEIGTGISLPMAHPLICVPVSHSTPFVA 527

BLAST of CSPI03G29820 vs. NCBI nr
Match: XP_022949336.1 (cytochrome P450 705A22-like [Cucurbita moschata])

HSP 1 Score: 905.2 bits (2338), Expect = 2.6e-259
Identity = 453/526 (86.12%), Postives = 484/526 (92.02%), Query Frame = 0

Query: 8   YTLYLLLFFLSSLLLFFFLHKPSKSAS-------ANLTLPPSPPPLPVIGHLHLLLPATH 67
           Y LY LLFFLS L L FFLHK S SAS       A LTLPPSPP LP+IGHLHLLLPA +
Sbjct: 4   YILYFLLFFLSCLFLLFFLHKTSGSASSSSSAAAAKLTLPPSPPSLPLIGHLHLLLPANY 63

Query: 68  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 127
           KSFFNISSKYGPLLHIRL +LQYVLVSSASLAAEIF+THDLTFSSRPDFAFSEEYPYGKV
Sbjct: 64  KSFFNISSKYGPLLHIRLCSLQYVLVSSASLAAEIFRTHDLTFSSRPDFAFSEEYPYGKV 123

Query: 128 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 187
           GFLGAPYGDYWRYMKKLTMMEVLA PQLARSRFVRNEEI RML KLLL S++K+SVDLGA
Sbjct: 124 GFLGAPYGDYWRYMKKLTMMEVLAPPQLARSRFVRNEEISRMLHKLLLSSQKKESVDLGA 183

Query: 188 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFW 247
           EL+KLTNNSICRMMMSTRCSEENNEAE IR LVNDTIEIATK+AFGDLF++GPLKRLPFW
Sbjct: 184 ELVKLTNNSICRMMMSTRCSEENNEAENIRKLVNDTIEIATKLAFGDLFSEGPLKRLPFW 243

Query: 248 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRN 307
           LFG K L++N+RFD+LLE ILQQHE+RAKIHG ER+D DLMDILLKAYLDEKAEFK+TRN
Sbjct: 244 LFGKKVLKLNIRFDVLLEKILQQHEERAKIHGFERDDRDLMDILLKAYLDEKAEFKITRN 303

Query: 308 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 367
           HIKAFLLDLFIAGTGTSAEVMQW +AEL+NHP+VF+KVR+EIE V G+RLVEETD+ NLP
Sbjct: 304 HIKAFLLDLFIAGTGTSAEVMQWTIAELINHPNVFRKVRQEIEYVVGSRLVEETDIVNLP 363

Query: 368 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 427
           Y QAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF
Sbjct: 364 YMQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 423

Query: 428 RPERFYNENSSKEE---GTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAAL 487
           RPERFYN N++K+E   G KHIQYEIKGQ+FSFVPFGGGRRGCPGSLLA+NTINRTVAAL
Sbjct: 424 RPERFYNANTNKDEGDQGMKHIQYEIKGQNFSFVPFGGGRRGCPGSLLAYNTINRTVAAL 483

Query: 488 VQCFDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVA 524
           VQCFDWKVGKDG+ EKVNMEIGTGISLPMAH  ICVPVSHSTPFVA
Sbjct: 484 VQCFDWKVGKDGNGEKVNMEIGTGISLPMAHAFICVPVSHSTPFVA 529

BLAST of CSPI03G29820 vs. NCBI nr
Match: XP_022998426.1 (cytochrome P450 705A22-like [Cucurbita maxima])

HSP 1 Score: 903.7 bits (2334), Expect = 7.6e-259
Identity = 453/524 (86.45%), Postives = 482/524 (91.98%), Query Frame = 0

Query: 8   YTLYLLLFFLSSLLLFFFLHKPSKSAS-----ANLTLPPSPPPLPVIGHLHLLLPATHKS 67
           Y LY LLFFLS L L F LHK S SAS     A LTLPPSPP LP+IGHLHLLLPA +KS
Sbjct: 4   YILYFLLFFLSCLFLLFLLHKTSGSASSSAAAAKLTLPPSPPSLPLIGHLHLLLPANYKS 63

Query: 68  FFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGF 127
           FFNISSKYGPLLHIRL +LQYVLVSSASLAAEIF+THDLTFSSRPDFAFSEEYPYGKVGF
Sbjct: 64  FFNISSKYGPLLHIRLCSLQYVLVSSASLAAEIFRTHDLTFSSRPDFAFSEEYPYGKVGF 123

Query: 128 LGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAEL 187
           LGAPYGDYWRYMKKLTMMEVLA PQLARSRFVRNEEI RML KLLL S++K+SVDLGAEL
Sbjct: 124 LGAPYGDYWRYMKKLTMMEVLAPPQLARSRFVRNEEISRMLHKLLLSSQKKESVDLGAEL 183

Query: 188 IKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLF 247
           IKLTNNSICRMMMSTRCSEENNEAE IR LVNDTIEIATK+AFGDLF++GPLKRLPFWLF
Sbjct: 184 IKLTNNSICRMMMSTRCSEENNEAENIRKLVNDTIEIATKLAFGDLFSEGPLKRLPFWLF 243

Query: 248 GNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHI 307
           G K L++N+RFD+LLE ILQQHE+RAKIHG ER+D DLMDILLKAYLDEKAEFK+TRNHI
Sbjct: 244 GKKVLKLNIRFDVLLEKILQQHEERAKIHGFERDDRDLMDILLKAYLDEKAEFKITRNHI 303

Query: 308 KAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLPYT 367
           KAFLLDLFIAGTGTSAEVMQW +AEL+NHP+VF+KVR+EIE V G+RLVEETD+ NLPY 
Sbjct: 304 KAFLLDLFIAGTGTSAEVMQWTIAELINHPNVFRKVRQEIECVVGSRLVEETDIVNLPYM 363

Query: 368 QAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRP 427
           QAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRP
Sbjct: 364 QAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRP 423

Query: 428 ERFYNENSSKEE---GTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQ 487
           ERFYN N +K+E   G KHIQYEIKGQ+FSFVPFGGGRRGCPGSLLA+NTINRTVAALVQ
Sbjct: 424 ERFYNANPNKDEGDQGMKHIQYEIKGQNFSFVPFGGGRRGCPGSLLAYNTINRTVAALVQ 483

Query: 488 CFDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVA 524
           CFDWKVGKDG+ EKVNMEIGTGISLPMAH  ICVPVSHSTPFVA
Sbjct: 484 CFDWKVGKDGNGEKVNMEIGTGISLPMAHSFICVPVSHSTPFVA 527

BLAST of CSPI03G29820 vs. TAIR 10
Match: AT3G20940.1 (cytochrome P450, family 705, subfamily A, polypeptide 30 )

HSP 1 Score: 446.8 bits (1148), Expect = 2.4e-125
Identity = 243/509 (47.74%), Postives = 334/509 (65.62%), Query Frame = 0

Query: 13  LLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPA-THKSFFNISSKYG 72
           +LFFL SLL +    +  K + A   LPPSPP  PVIGHLHLLL A  HKSF NISSKYG
Sbjct: 16  ILFFLFSLLCYSLFFRKPKGSRAGRDLPPSPPSFPVIGHLHLLLSALVHKSFQNISSKYG 75

Query: 73  PLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGFLGAPYGDYW 132
           PLLH+R+  +  VL SSAS+A EIFK  D+  SSR      E   +G   F  APYGDY+
Sbjct: 76  PLLHLRVFHIPIVLASSASVAYEIFKAQDVNVSSRGHAPVGESLWFGSSSFFFAPYGDYF 135

Query: 133 RYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAELIKLTNNSIC 192
           ++M+KL   ++L    L RSR +R +E+ R  + LL  + +K+SV++G E  KL NN IC
Sbjct: 136 KFMRKLIATKLLGPQALERSRKIRADELDRFYKTLLDKAMKKESVEIGEEAAKLNNNIIC 195

Query: 193 RMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLFGNKALQINV 252
           +M+M   CSEEN EAEK R LV +++ +  ++ FG +F + PLK+L   LF    L ++ 
Sbjct: 196 KMIMGRSCSEENGEAEKFRHLVIESMALTKQIFFGMIFHK-PLKKLGISLFQKDILSLSR 255

Query: 253 RFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHIKAFLLDLFI 312
           +FD LLE IL +HE++   H    +  D+MD LL+AY DE AE+K+TRNHIK+  +DL I
Sbjct: 256 KFDELLEKILFEHEEKKAEH---NQANDMMDFLLEAYGDENAEYKITRNHIKSLFVDLVI 315

Query: 313 AGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAG-TRLVEETDVTNLPYTQAVVKECL 372
           AGT TS +  QW M EL+N+P + Q++R EIESV G TRL++E D+ NLPY QAVVKE L
Sbjct: 316 AGTDTSVQATQWTMGELINNPKILQRLREEIESVVGNTRLIQENDLPNLPYLQAVVKEGL 375

Query: 373 RLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRPERFYNENS 432
           RL+P   ++ R  +E C++ G+ IP+  ++ V+ +AIMRDPN WE+P+EF+PERF   + 
Sbjct: 376 RLHPPGSISVRMFQERCELKGFYIPEKTLLVVNTYAIMRDPNFWEDPEEFKPERFIASSR 435

Query: 433 SKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQCFDWKVGKDGD 492
           S++E       E++ +   ++PF  GRRGCPGS LA+ ++   +  +VQCFDW++    +
Sbjct: 436 SEQED------EVREEVLKYIPFSAGRRGCPGSNLAYISLGIVIGVMVQCFDWRI----E 495

Query: 493 EEKVNM-EIGTGISLPMAHPLICVPVSHS 519
            EKVNM E     +L MA PL C PVS +
Sbjct: 496 GEKVNMNEAAETTALSMAQPLKCTPVSRT 510

BLAST of CSPI03G29820 vs. TAIR 10
Match: AT2G42250.1 (cytochrome P450, family 712, subfamily A, polypeptide 1 )

HSP 1 Score: 446.0 bits (1146), Expect = 4.1e-125
Identity = 247/523 (47.23%), Postives = 344/523 (65.77%), Query Frame = 0

Query: 3   DAALHYTLYLLLFFLSSLLLFFFLHK---PSKSASANLTLPPSPPPLPVIGHLHLLLPAT 62
           D  L+  L +L+  L+   + + L K     + + A   LP SPP LP IGHLHL+    
Sbjct: 2   DLKLNTKLIILITSLAFPFMLYALFKWFLKEQGSLAATKLPQSPPALPFIGHLHLIGKVL 61

Query: 63  HKSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGK 122
             SF +++ KYGPL+ IRLGA + V+VSS+S+A EIFK  +L FSSRP+F  +E + Y  
Sbjct: 62  PVSFQSLAHKYGPLMEIRLGASKCVVVSSSSVAREIFKEQELNFSSRPEFGSAEYFKYRG 121

Query: 123 VGFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLG 182
             F+ A YGDYWR+MKKL M ++LA PQL +   +R EE L+++  +  C ++    DL 
Sbjct: 122 SRFVLAQYGDYWRFMKKLCMTKLLAVPQLEKFADIREEEKLKLVDSVAKCCREGLPCDLS 181

Query: 183 AELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPF 242
           ++ IK TNN ICRM MSTRCS  +NEAE+IR LV  ++E+A K++ GD+   GPLK + F
Sbjct: 182 SQFIKYTNNVICRMAMSTRCSGTDNEAEEIRELVKKSLELAGKISVGDVL--GPLKVMDF 241

Query: 243 WLFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTR 302
              G K + +  ++DLL+E I+++ E +AK     R+  D++DILL+ Y D  AE K+TR
Sbjct: 242 SGNGKKLVAVMEKYDLLVERIMKEREAKAKKKDGTRK--DILDILLETYRDPTAEMKITR 301

Query: 303 NHIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGT-RLVEETDVTN 362
           N +K+FLLD+F+AGT TSA  MQWAM +L+NHP  F K+R EI +V G+ RLV+E+DV N
Sbjct: 302 NDMKSFLLDVFMAGTDTSAAAMQWAMGQLINHPQAFNKLREEINNVVGSKRLVKESDVPN 361

Query: 363 LPYTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPD 422
           LPY +AV++E LRL+P+ P+  R C E C+VNG  +     V V+++AIMRD  LW + D
Sbjct: 362 LPYLRAVLRETLRLHPSAPLIIRECAEDCQVNGCLVKSKTRVLVNVYAIMRDSELWADAD 421

Query: 423 EFRPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALV 482
            F PERF    SS+E+  +H Q + KGQ+F ++PFG GRRGCPG+ LA N ++  V +LV
Sbjct: 422 RFIPERFL--ESSEEKIGEH-QMQFKGQNFRYLPFGSGRRGCPGASLAMNVMHIGVGSLV 481

Query: 483 QCFDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPF 522
           Q FDW   K  D +KV++  G+G S  MA PL+C PV H   F
Sbjct: 482 QRFDW---KSVDGQKVDLSQGSGFSAEMARPLVCNPVDHFNTF 514

BLAST of CSPI03G29820 vs. TAIR 10
Match: AT4G15350.1 (cytochrome P450, family 705, subfamily A, polypeptide 2 )

HSP 1 Score: 440.7 bits (1132), Expect = 1.7e-123
Identity = 233/509 (45.78%), Postives = 334/509 (65.62%), Query Frame = 0

Query: 10  LYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLL--PATHKSFFNIS 69
           +++LL  LS L    F  KP  S      LPPSPP LP+IGHLHL+L    THKSF  +S
Sbjct: 6   IFILLCLLSFLCYSLFFMKPKDSRDGR-DLPPSPPSLPIIGHLHLILLSTLTHKSFQRLS 65

Query: 70  SKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGFLGAPY 129
           SKYGPLLH+R+  +  VL SSAS+A EIF+  D+  S R      E    G   F+ APY
Sbjct: 66  SKYGPLLHLRIFHVPIVLASSASVAYEIFRDQDVNVSFRHSPPIEESLFLGSYSFISAPY 125

Query: 130 GDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAELIKLTN 189
           GDYW++M+KL + ++L    L RSR  R +E+ R  + LL  + +K+SV++  E  KL N
Sbjct: 126 GDYWKFMRKLMVTKILGPQALERSRRFREDELDRFYKTLLDKAMKKESVEIVEEAAKLNN 185

Query: 190 NSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLFGNKAL 249
           N+IC+M+M   CSEE  EAE+IR LV +++ +  K+    +F + PLK+L   LF  + +
Sbjct: 186 NTICKMIMGRSCSEETGEAERIRGLVTESMALTKKIFLATIFHK-PLKKLGISLFKKEIM 245

Query: 250 QINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHIKAFLL 309
            ++ +FD LLE IL +HE++ + H    +  D+MD+LL+AY DE AE+K+TRNHIK+  +
Sbjct: 246 SVSRKFDELLEKILVEHEEKMEEH---HQGTDMMDVLLEAYRDENAEYKITRNHIKSMFV 305

Query: 310 DLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAG-TRLVEETDVTNLPYTQAVV 369
           DLFIAGT TS+  +QW MAE++NHP + +++R EI+ V G TRL++ETD+ NL Y QA++
Sbjct: 306 DLFIAGTDTSSTTIQWIMAEIINHPKILERLREEIDFVVGKTRLIQETDLPNLLYLQAII 365

Query: 370 KECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRPERFY 429
           KE LRL+P  P+  R  +E C++ G+ IP+  ++ V+ +AIMRDP+ WE+PDEF+PERF 
Sbjct: 366 KEGLRLHPPGPLLPRTVQERCEIKGFHIPEKTILVVNSYAIMRDPDFWEDPDEFKPERFL 425

Query: 430 NENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQCFDWKVG 489
           + + S +E       EI+ +   ++PF  GRRGCPG+ LA+ ++   V  +VQCFDWK+ 
Sbjct: 426 SISRSGQED------EIRDKFLKYIPFASGRRGCPGTNLAYASVGTAVGVMVQCFDWKI- 485

Query: 490 KDGDEEKVNMEIGTGISLPMAHPLICVPV 516
            +G+   +N   GT + L MAHPL C PV
Sbjct: 486 -EGENVNMNEAAGTMV-LTMAHPLKCTPV 500

BLAST of CSPI03G29820 vs. TAIR 10
Match: AT3G20130.1 (cytochrome P450, family 705, subfamily A, polypeptide 22 )

HSP 1 Score: 434.9 bits (1117), Expect = 9.3e-122
Identity = 229/514 (44.55%), Postives = 338/514 (65.76%), Query Frame = 0

Query: 10  LYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPA-THKSFFNISS 69
           +++L+F L+ L  FFF  KP K +  N  LPPSPP LP+IGH+HLLL   THKS   +SS
Sbjct: 14  IFILIFLLTFLCFFFFFKKP-KDSRVNFDLPPSPPSLPIIGHVHLLLSTLTHKSLQKLSS 73

Query: 70  KYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGFLGAPYG 129
           +YGPLL++R+  +  +LVSSAS+A EIF+T D+  SSR   A  E   +G   F+ APYG
Sbjct: 74  RYGPLLYLRIFNVPIILVSSASVAYEIFRTQDVNISSRGVTAVDESLVFGSSSFVTAPYG 133

Query: 130 DYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAELIKLTNN 189
           DYW++MKKLT+M++L      +SR +R ++I R  + LL  +++K+SV++G E + L NN
Sbjct: 134 DYWKFMKKLTVMKLLGPQAQEQSRDIRADDIKRFCRNLLDKARKKESVEIGKEAMNLMNN 193

Query: 190 SICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLFGNKALQ 249
            +C+M M    SEEN E EK+R LV ++I +  KM F  +  +  L++L   LF    + 
Sbjct: 194 ILCKMSMGRSFSEENGETEKLRGLVTESIGLMKKM-FLAVLLRRQLQKLGISLFKKDIMG 253

Query: 250 INVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHIKAFLLD 309
           ++ +FD+LLE +L +H ++ +    + +   ++D+LL AY DE AE+K+T+NHIKAF +D
Sbjct: 254 VSNKFDVLLEKVLVEHREKPE----KDQGTVMLDVLLAAYGDENAEYKITKNHIKAFFVD 313

Query: 310 LFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAG-TRLVEETDVTNLPYTQAVVK 369
           LFI  T TS + +QW MAE+MN+  + +++R EI+SV G +RL++ETD+ NLPY  AV+K
Sbjct: 314 LFIGATDTSVQTIQWTMAEIMNNTHILERMREEIDSVVGKSRLIQETDLPNLPYLHAVIK 373

Query: 370 ECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRPERFYN 429
           E LRL+P  P+  R  ++ CK+ G+ IP+   + ++ + +MRDPN+WE+P+EF+PERF  
Sbjct: 374 EALRLHPPGPLLPREFQQGCKIGGFYIPEKTTLLINAYVVMRDPNVWEDPEEFKPERFLA 433

Query: 430 ENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQCFDWKVGK 489
            + S +E       E + Q+  F+PFG GRRGCPGS LA+  +   +  +VQCFDW++  
Sbjct: 434 SSRSGQED------ERREQALKFLPFGSGRRGCPGSNLAYMIVGSAIGMMVQCFDWRI-- 493

Query: 490 DGDEEKVNM-EIGTGISLPMAHPLICVPVSHSTP 521
             + EKVNM E   G  L MAHPL   PV+   P
Sbjct: 494 --EGEKVNMKEAVKGTILTMAHPLKLTPVTRQPP 511

BLAST of CSPI03G29820 vs. TAIR 10
Match: AT3G20100.1 (cytochrome P450, family 705, subfamily A, polypeptide 19 )

HSP 1 Score: 433.7 bits (1114), Expect = 2.1e-121
Identity = 238/511 (46.58%), Postives = 337/511 (65.95%), Query Frame = 0

Query: 10  LYLLLFFLSSLLLFFFLHKPSKSASAN-LTLPPSPPPLPVIGHLHLLLPA-THKSFFNIS 69
           ++LLL   S +    F  KPS    A+   LPPSPP LPVIGHLHL+L +  HKSF  IS
Sbjct: 11  IFLLLCLFSLVCYSLFFRKPSSRRDAHGCDLPPSPPSLPVIGHLHLILSSLVHKSFQKIS 70

Query: 70  SKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGFLGAPY 129
           S YGPLLH+R+  +  VLVSSAS+A +IF+ HDL  SSR    F E   +G  GF+ APY
Sbjct: 71  SNYGPLLHLRIFNVPIVLVSSASVAYDIFRVHDLNVSSRGSPPFEESLLFGSTGFISAPY 130

Query: 130 GDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAELIKLTN 189
           GDY+++MKK  + ++L    L RSR +R  E+ R    LL  + +K+SV++G E +KL+N
Sbjct: 131 GDYFKFMKKHLVTKLLGPQALERSRLIRTNELERFYINLLDKATKKESVEIGKEAMKLSN 190

Query: 190 NSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLFGNKAL 249
           NSIC+M+M   C EE  EAE++R L+ ++  + TK  F     +G L++L   LF  + +
Sbjct: 191 NSICKMIMGRSCLEEKGEAERVRGLIIESFYL-TKKFFLAFTLRGLLEKLGISLFKKEIM 250

Query: 250 QINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHIKAFLL 309
            ++ RFD LLE  L++HE++      E +D D++D LL AY DEKAE+K+TRN IKAFL+
Sbjct: 251 GVSRRFDDLLERYLREHEEKP---DNEHQDTDMIDALLAAYRDEKAEYKITRNQIKAFLV 310

Query: 310 DLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAG-TRLVEETDVTNLPYTQAVV 369
           D+FIAGT  SA   Q  MAE++N+P++F ++R EI+SV G +RL++ETD+  LPY QAVV
Sbjct: 311 DIFIAGTDISALTTQGTMAEIINNPNIFVRIREEIDSVVGKSRLIQETDLPKLPYLQAVV 370

Query: 370 KECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRPERFY 429
           KE LRL+P  P+  R  +E CKV G+ IP    + V+ +A+MRDPN+WE+P+EF+PERF 
Sbjct: 371 KEGLRLHPPTPLMVREFQEGCKVKGFYIPASTTLVVNGYAVMRDPNVWEDPEEFKPERFL 430

Query: 430 NENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQCFDWKVG 489
             +   +E       EI+ Q+  ++ FG GRRGCPG+ +A+  +   +  +VQCFDW++ 
Sbjct: 431 ASSRLMQED------EIREQALKYIAFGSGRRGCPGANVAYIFVGTAIGMMVQCFDWRI- 490

Query: 490 KDGDEEKVNM-EIGTGISLPMAHPLICVPVS 517
              + EKV+M E   G++L +AHPL C PV+
Sbjct: 491 ---NGEKVDMKEAIGGLNLTLAHPLKCTPVA 507

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LJY51.3e-12044.55Cytochrome P450 705A22 OS=Arabidopsis thaliana OX=3702 GN=CYP705A22 PE=2 SV=1[more]
Q9FI391.6e-11846.46Cytochrome P450 705A5 OS=Arabidopsis thaliana OX=3702 GN=CYP705A5 PE=2 SV=1[more]
O819731.2e-11044.38Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1[more]
Q9LJY76.1e-11044.02Cytochrome P450 705A20 OS=Arabidopsis thaliana OX=3702 GN=CYP705A20 PE=2 SV=1[more]
Q9FH672.8e-10742.75Cytochrome P450 705A12 OS=Arabidopsis thaliana OX=3702 GN=CYP705A12 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L9V63.9e-30199.62Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa3G698490 PE=2 SV=1[more]
A0A5D3CH285.1e-29396.75Cytochrome P450 705A5-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CGU35.1e-29396.75cytochrome P450 705A5-like OS=Cucumis melo OX=3656 GN=LOC103500305 PE=3 SV=1[more]
A0A6J1GBR61.3e-25986.12cytochrome P450 705A22-like OS=Cucurbita moschata OX=3662 GN=LOC111452721 PE=3 S... [more]
A0A6J1KA723.7e-25986.45cytochrome P450 705A22-like OS=Cucurbita maxima OX=3661 GN=LOC111493061 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
NP_001292616.18.0e-30199.62cytochrome P450 705A5-like [Cucumis sativus] >AIT72034.1 cytochrome P450 [Cucumi... [more]
XP_008461786.11.1e-29296.75PREDICTED: cytochrome P450 705A5-like [Cucumis melo] >KAA0048327.1 cytochrome P4... [more]
XP_038903604.18.7e-27188.80cytochrome P450 705A22-like [Benincasa hispida][more]
XP_022949336.12.6e-25986.12cytochrome P450 705A22-like [Cucurbita moschata][more]
XP_022998426.17.6e-25986.45cytochrome P450 705A22-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G20940.12.4e-12547.74cytochrome P450, family 705, subfamily A, polypeptide 30 [more]
AT2G42250.14.1e-12547.23cytochrome P450, family 712, subfamily A, polypeptide 1 [more]
AT4G15350.11.7e-12345.78cytochrome P450, family 705, subfamily A, polypeptide 2 [more]
AT3G20130.19.3e-12244.55cytochrome P450, family 705, subfamily A, polypeptide 22 [more]
AT3G20100.12.1e-12146.58cytochrome P450, family 705, subfamily A, polypeptide 19 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 91..112
score: 23.95
coord: 67..86
score: 31.3
coord: 450..460
score: 54.96
coord: 460..483
score: 29.12
coord: 301..318
score: 37.76
coord: 321..347
score: 35.12
coord: 185..203
score: 21.97
coord: 363..381
score: 32.84
IPR001128Cytochrome P450PRINTSPR00385P450coord: 312..329
score: 30.8
coord: 451..460
score: 56.5
coord: 364..375
score: 47.23
IPR001128Cytochrome P450PFAMPF00067p450coord: 40..504
e-value: 3.5E-90
score: 303.0
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 33..513
e-value: 3.7E-114
score: 383.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 36..514
NoneNo IPR availablePIRSRPIRSR000209-1PIRSR000209-1coord: 41..484
e-value: 2.2E-33
score: 112.8
NoneNo IPR availablePANTHERPTHR24298:SF59CYTOCHROME P450, FAMILY 705, SUBFAMILY A, POLYPEPTIDE 25-RELATEDcoord: 7..521
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 7..521
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 453..462

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G29820.1CSPI03G29820.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen