CSPI03G29390 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G29390
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	Chr3: 26963694 .. 26964889 (-)
RNA-Seq Expression	CSPI03G29390
Synteny	CSPI03G29390

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCCTGCATGCCTTAAAAAGAGGGACAACAAAGAAAGCAAGAACAAAGGAGAAGAAAGAACACAAACACAAACCTCATCATACATTCCACCATTTTTTTTTAACTCTTCTTTAATCTCCTTCGTCATTCTTCCTTCCCTTTTAACAATGGCCTCCGAGATCCATAGCCTCCTTCAAACTCTCTTCTTAGTTTCATCATCTCTTTCTCTCTGTTTCTCAATCAGAAACTCAATCCCGAATGGCTTCCTCAAATTCCTCTTAATTCTCCCCTTCTTCTTCCTCTTCCTCTACATTCCTCTCCAATTCCATACCATCCATTTCCAAGGCCCCATCGGCTTTTTCATTGGTTGGCTCGGCAGTTTCAAGCTCCTCCTCTTTGCCTTCGGTAAAGGCCCTCTCTGCTCCGCCGCCACCTCCTCCTCCCTCCCCCGCTTCCTCGCCGTCGGCTCCCTCCCAATCGAAATCCCCGACCACAAATCCCCCCCAAATCATTCCCTTATCCCCCCTTCCGCAAAATTAGTATTCCTAATCCTCACAATCCTCGCCATCAATTTCAAAAATCACCTCCATCCAAACGCAATTCCCCTGTTCTACTGCCTCCTAATATACTTCTTCCTCGAATTCCTCATCGGCACCACCGCCACCCTAGCCAAAACAGTTCTCGGAGTCGAGTTATTGCCCTATTTCAACGAGCCCTATTTCTCTGATTCGTTACAGGATTTTTGGGGACGACGATGGAATCTAATGACCTCTAGAATCCTTCGCCTCAGCATTTACGATCCATGCCGGAATCTAACCATCGGCGTCATCGGGAGGAGACCGGCGTCAATGGTGGCGGTGATAGCGACATTTGTAGTCTCTGGTCTTATGCACGAATTGATATATTTCTATATGGGAAGAATGGCGCCGACTTGGGAGGTGACTTGCTTCTTTGTGGTACATGGAGTTTGTGTGGTGGCGGAGATGGCGGTGAGGTTAGGCGCCGGTGGGAGGTTCCGGGCGCCGCGAGTTGCAAGAATATGTTTAACGATAATGTTTGTGATGGGAACGGGAAGTTGGCTTTTTTTCCCGCAGTGTATTAGGGCTAAAATGGACGTTAGAATGCTTGAAGAGTACGCCGCAATAGGTGCGTTTTTTAAGACTATTACGCTCCATTTCATCCCACCCTTTAACCCTACTTCTTTTCTATTCCATTAA

mRNA sequence

GCCCTGCATGCCTTAAAAAGAGGGACAACAAAGAAAGCAAGAACAAAGGAGAAGAAAGAACACAAACACAAACCTCATCATACATTCCACCATTTTTTTTTAACTCTTCTTTAATCTCCTTCGTCATTCTTCCTTCCCTTTTAACAATGGCCTCCGAGATCCATAGCCTCCTTCAAACTCTCTTCTTAGTTTCATCATCTCTTTCTCTCTGTTTCTCAATCAGAAACTCAATCCCGAATGGCTTCCTCAAATTCCTCTTAATTCTCCCCTTCTTCTTCCTCTTCCTCTACATTCCTCTCCAATTCCATACCATCCATTTCCAAGGCCCCATCGGCTTTTTCATTGGTTGGCTCGGCAGTTTCAAGCTCCTCCTCTTTGCCTTCGGTAAAGGCCCTCTCTGCTCCGCCGCCACCTCCTCCTCCCTCCCCCGCTTCCTCGCCGTCGGCTCCCTCCCAATCGAAATCCCCGACCACAAATCCCCCCCAAATCATTCCCTTATCCCCCCTTCCGCAAAATTAGTATTCCTAATCCTCACAATCCTCGCCATCAATTTCAAAAATCACCTCCATCCAAACGCAATTCCCCTGTTCTACTGCCTCCTAATATACTTCTTCCTCGAATTCCTCATCGGCACCACCGCCACCCTAGCCAAAACAGTTCTCGGAGTCGAGTTATTGCCCTATTTCAACGAGCCCTATTTCTCTGATTCGTTACAGGATTTTTGGGGACGACGATGGAATCTAATGACCTCTAGAATCCTTCGCCTCAGCATTTACGATCCATGCCGGAATCTAACCATCGGCGTCATCGGGAGGAGACCGGCGTCAATGGTGGCGGTGATAGCGACATTTGTAGTCTCTGGTCTTATGCACGAATTGATATATTTCTATATGGGAAGAATGGCGCCGACTTGGGAGGTGACTTGCTTCTTTGTGGTACATGGAGTTTGTGTGGTGGCGGAGATGGCGGTGAGGTTAGGCGCCGGTGGGAGGTTCCGGGCGCCGCGAGTTGCAAGAATATGTTTAACGATAATGTTTGTGATGGGAACGGGAAGTTGGCTTTTTTTCCCGCAGTGTATTAGGGCTAAAATGGACGTTAGAATGCTTGAAGAGTACGCCGCAATAGGTGCGTTTTTTAAGACTATTACGCTCCATTTCATCCCACCCTTTAACCCTACTTCTTTTCTATTCCATTAA

Coding sequence (CDS)

ATGGCCTCCGAGATCCATAGCCTCCTTCAAACTCTCTTCTTAGTTTCATCATCTCTTTCTCTCTGTTTCTCAATCAGAAACTCAATCCCGAATGGCTTCCTCAAATTCCTCTTAATTCTCCCCTTCTTCTTCCTCTTCCTCTACATTCCTCTCCAATTCCATACCATCCATTTCCAAGGCCCCATCGGCTTTTTCATTGGTTGGCTCGGCAGTTTCAAGCTCCTCCTCTTTGCCTTCGGTAAAGGCCCTCTCTGCTCCGCCGCCACCTCCTCCTCCCTCCCCCGCTTCCTCGCCGTCGGCTCCCTCCCAATCGAAATCCCCGACCACAAATCCCCCCCAAATCATTCCCTTATCCCCCCTTCCGCAAAATTAGTATTCCTAATCCTCACAATCCTCGCCATCAATTTCAAAAATCACCTCCATCCAAACGCAATTCCCCTGTTCTACTGCCTCCTAATATACTTCTTCCTCGAATTCCTCATCGGCACCACCGCCACCCTAGCCAAAACAGTTCTCGGAGTCGAGTTATTGCCCTATTTCAACGAGCCCTATTTCTCTGATTCGTTACAGGATTTTTGGGGACGACGATGGAATCTAATGACCTCTAGAATCCTTCGCCTCAGCATTTACGATCCATGCCGGAATCTAACCATCGGCGTCATCGGGAGGAGACCGGCGTCAATGGTGGCGGTGATAGCGACATTTGTAGTCTCTGGTCTTATGCACGAATTGATATATTTCTATATGGGAAGAATGGCGCCGACTTGGGAGGTGACTTGCTTCTTTGTGGTACATGGAGTTTGTGTGGTGGCGGAGATGGCGGTGAGGTTAGGCGCCGGTGGGAGGTTCCGGGCGCCGCGAGTTGCAAGAATATGTTTAACGATAATGTTTGTGATGGGAACGGGAAGTTGGCTTTTTTTCCCGCAGTGTATTAGGGCTAAAATGGACGTTAGAATGCTTGAAGAGTACGCCGCAATAGGTGCGTTTTTTAAGACTATTACGCTCCATTTCATCCCACCCTTTAACCCTACTTCTTTTCTATTCCATTAA

Protein sequence

MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQGPIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPPSAKLVFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVIATFVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPFNPTSFLFH*

Homology

[Close]

BLAST of CSPI03G29390 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 4.0e-65
Identity = 139/332 (41.87%), Postives = 192/332 (57.83%), Query Frame = 0


Query: 7   SLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQGPIGFFI 66
           S ++   LV  SL   F I   +P G  + +L  P F +F  +P   +++H  G   FFI
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  GWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIP---------DHKSPPNHSL 126
            WL +FKLLLFA G+GPL S     SLP FLAV  LPI+I           H+      L
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPL 122

Query: 127 IPPSAKLVFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELL 186
           I  + K VF++L I A  +   L    +   Y + IYF LE ++  TA   + +  +EL 
Sbjct: 123 I-YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELE 182

Query: 187 PYFNEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVIATFVV 246
           P FN+PY + SLQDFWGRRWNLM + ILR ++Y+P   L   V+G   + ++A   TFVV
Sbjct: 183 PQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQL-FSVLGPNYSQILAAFGTFVV 242

Query: 247 SGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMF 306
           SG+MHELI+FYMGR+ P W++  FF+++G C   E+A++    GR+R P+     LT+ F
Sbjct: 243 SGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTF 302

Query: 307 VMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAF 330
           VM T  WLF P+  R  +  + L+EYAAIGAF
Sbjct: 303 VMVTALWLFLPEFNRCNIVEKALDEYAAIGAF 332

[Close]

BLAST of CSPI03G29390 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 3.1e-57
Identity = 125/303 (41.25%), Postives = 171/303 (56.44%), Query Frame = 0


Query: 32  GFLKFLLILPFFFLFLYIPLQFHTIHFQGPIGFFIGWLGSFKLLLFAFGKGPLCSAATSS 91
           G L+ LL+LP   LF+++PL+  + H  GP   ++ WL +FKLLLFAF  GPL +   S 
Sbjct: 35  GALRLLLLLPVVLLFIFLPLRLSSFHLGGPTALYLVWLANFKLLLFAFHLGPLSN--PSL 94

Query: 92  SLPRFLAVGSLPIEIPDHKSPPN-----------HSLIPPSAKLVFLILTILAINFKNHL 151
           SL  F++   LPI+  D  S  +             ++   AKLVF    +    F+  L
Sbjct: 95  SLLHFISTTLLPIKFRDDPSNDHEKNKRTLSFEWRKVVLFVAKLVFFAGILKIYEFRKDL 154

Query: 152 HPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYFNEPYFSDSLQDFWGRRWNLM 211
               I + YC   Y   E  +  +A +A+  LG++L P FNEPY + SLQDFWGRRWNLM
Sbjct: 155 PHFVISVLYCFHFYLGTEITLAASAVIARATLGLDLYPQFNEPYLATSLQDFWGRRWNLM 214

Query: 212 TSRILRLSIYDPCRNLTIGVIGRRPASMVAVIATFVVSGLMHELIYFYMGRMAPTWEVTC 271
            S IL L+ Y P R +    +  R     A++  F VSGLMHE+ +FY+ R  P+WEVT 
Sbjct: 215 VSDILGLTTYQPVRRVLSRWVRLRWEVAGAMLVAFTVSGLMHEVFFFYLTRARPSWEVTG 274

Query: 272 FFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMGTGSWLFFPQCIRAKMDVRML 324
           FFV+HGVC   EM V+    G+ R  R     LT+ FVM TG WLF PQ +R  +D++ +
Sbjct: 275 FFVLHGVCTAVEMVVKKAVSGKVRLRREVSGALTVGFVMVTGGWLFLPQLVRHGVDLKTI 334

[Close]

BLAST of CSPI03G29390 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.4e-56
Identity = 131/335 (39.10%), Postives = 184/335 (54.93%), Query Frame = 0


Query: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           M  E+ SL++       S+S C+     I +G  + L +LP   LFL +PL   ++HF G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
              FF+ WL +FKL+LF+F +GPL      S+L RF+     PI++  +  P N   +P 
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPL--FPVPSNLSRFVCFTCFPIKLQQNPKPQNQ--MPK 120

Query: 121 ---SAKLVFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELL 180
              + KL F  + +    +K H+ P  + + Y L IY   E L+     L    L  +L 
Sbjct: 121 WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDLE 180

Query: 181 PYFNEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVIATFVV 240
           P+FNEPY S SLQDFWGRRWNLM   ILR ++Y P R +    +    A  + V A+F+V
Sbjct: 181 PHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFLV 240

Query: 241 SGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAV-RLGAGGRFRAPRVARICLTIM 300
           SG++HELI+FY  R +PT EVT FFV+HGVC  AE A  R     R++  ++    LT+ 
Sbjct: 241 SGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLTVG 300

Query: 301 FVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFFK 332
           FV+ TG WLFFP   R+ M  R+ +E      F K
Sbjct: 301 FVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVK 331

[Close]

BLAST of CSPI03G29390 vs. ExPASy Swiss-Prot
Match: Q9FJ73 (Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=AT2 PE=3 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.5e-51
Identity = 124/335 (37.01%), Postives = 186/335 (55.52%), Query Frame = 0


Query: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           M  E+ +L++       S+S C+ I + I  G L+ L +LP F +FL +PL F ++HF  
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
             GFF  WL +FKL LFAF + PL  +   S+L RF      PI+I  +K+P ++ +   
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL--SPLPSNLTRFFCFACFPIKI--NKNPSSNRIHNK 120

Query: 121 SAKLVFLILTILAINFKNHLHPN---------AIPLFYCLLIYFFLEFLIGTTATLAKTV 180
                 L   +L  +F  H++ N         A    + + +Y   E ++     L   +
Sbjct: 121 PMSKWVLAFKLLIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSML 180

Query: 181 LGVELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAV 240
           LG E+ P FN+PY + SLQ+FW RRWNLM   +LR +++ P +     ++G   A    +
Sbjct: 181 LGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAGM 240

Query: 241 IATFVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFR---APRV 300
           +ATF+VSGLMHELIYFY+ R +PTWEVTCFF++HGV    E+A++     R R    PR 
Sbjct: 241 LATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMK-----RMRWLPTPRR 300

Query: 301 ARICLTI-MFVMGTGSWLFFPQCIRAKMDVRMLEE 323
           A   L I +F++ T  WLF+PQ +R  +  R++ E
Sbjct: 301 AVSGLAITVFLLVTAGWLFYPQMLRNDVHKRVISE 326

[Close]

BLAST of CSPI03G29390 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.2e-50
Identity = 126/331 (38.07%), Postives = 177/331 (53.47%), Query Frame = 0


Query: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           M  +  +L++       SLS C+ I + +  G L+ L ILP   LFL +PL    +HF  
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSP---PNHSL 120
               F+ WL +FKLLLFAF +GPL        L RF+    LPI+I    SP   PN   
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPL--FPLPPKLSRFICFACLPIKIRQDPSPNAIPNLHP 120

Query: 121 IPPSAKLVFLILTILAINFKNHLHPNAIPLF-----YCLLIYFFLEFLIGTTATLAKTVL 180
            P    ++ + + +L +    + + + +P F     YCL IY  +E ++     +  T+L
Sbjct: 121 KPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLL 180

Query: 181 GVELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVI 240
           G  + P FNEPY + SLQDFW RRWNLM S +LR +++ P +     ++    A    V+
Sbjct: 181 GCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVM 240

Query: 241 ATFVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARIC 300
           A+F VSGLMHEL+YFYM R  PTWEVTCFFV+HG     E+AV+       R P  A   
Sbjct: 241 ASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRTQW--LRPPHRAVSG 300

Query: 301 LTIM-FVMGTGSWLFFPQCIRAKMDVRMLEE 323
           L ++ FV  TG WLF  Q +R  +  + + E
Sbjct: 301 LVVLTFVSVTGVWLFLAQVLRNNVHEKAIGE 327

[Close]

BLAST of CSPI03G29390 vs. ExPASy TrEMBL
Match: A0A0A0L9R8 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G683670 PE=3 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 2.3e-196
Identity = 346/349 (99.14%), Postives = 348/349 (99.71%), Query Frame = 0


Query: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGF KFLLILPFFFLFLYIPLQFHTIHFQG
Sbjct: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFFKFLLILPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
           PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120

Query: 121 SAKLVFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180
           SAKL+FLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF
Sbjct: 121 SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180

Query: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240
           NEPYFSDSLQDFWGRRWNLMTSRILRL+IYDPCRNLTIGVIGRRPASMVAVIATFVVSGL
Sbjct: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240

Query: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300
           MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG
Sbjct: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300

Query: 301 TGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPFNPTSFLFH 350
           TGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPFNPTSFLFH
Sbjct: 301 TGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPFNPTSFLFH 349

[Close]

BLAST of CSPI03G29390 vs. ExPASy TrEMBL
Match: A0A5D3BC71 (Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold939G00050 PE=3 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 1.5e-171
Identity = 308/348 (88.51%), Postives = 326/348 (93.68%), Query Frame = 0


Query: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MA+EIHSLLQTLFLVSSSLS+CFSIRNSIPNGFLKFLL+LPFFFLFLYIPLQFHTIHFQG
Sbjct: 1   MAAEIHSLLQTLFLVSSSLSICFSIRNSIPNGFLKFLLVLPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
           PIGFFIGWLGSFKLLLFAFG+GPLCSAATSSSLPRFLAVGSLPIEIPDHKSPP+HSL+PP
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGRGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPHHSLLPP 120

Query: 121 SAKLVFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180
           SAKL FLILTILAINFKNH H NA+ LFYCLLIYFFLEFLIGTTA LAK VLGVELLPYF
Sbjct: 121 SAKLGFLILTILAINFKNHFHQNAVLLFYCLLIYFFLEFLIGTTAALAKKVLGVELLPYF 180

Query: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240
           NEPY SDSLQDFWGRRWNLMTSRILRL++YDPCRNLT+G+IGRR ASMVAV+ATF VSGL
Sbjct: 181 NEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRNLTVGIIGRRRASMVAVMATFGVSGL 240

Query: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300
           MHELIYFYMGRMAPTWEV+CFFVVHGVCVVAE AV+LGAGGR+RAPR  RI LTI FVMG
Sbjct: 241 MHELIYFYMGRMAPTWEVSCFFVVHGVCVVAERAVKLGAGGRYRAPRAVRIGLTIGFVMG 300

Query: 301 TGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPFNPTSFLF 349
           TGSWLFFPQCIRAKMDVRMLEEYAA+GAF K IT+ FI   +  SFLF
Sbjct: 301 TGSWLFFPQCIRAKMDVRMLEEYAAMGAFLKDITVLFI---DSISFLF 345

[Close]

BLAST of CSPI03G29390 vs. ExPASy TrEMBL
Match: A0A1S3CG36 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC103500343 PE=3 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 1.5e-171
Identity = 308/348 (88.51%), Postives = 326/348 (93.68%), Query Frame = 0


Query: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MA+EIHSLLQTLFLVSSSLS+CFSIRNSIPNGFLKFLL+LPFFFLFLYIPLQFHTIHFQG
Sbjct: 1   MAAEIHSLLQTLFLVSSSLSICFSIRNSIPNGFLKFLLVLPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
           PIGFFIGWLGSFKLLLFAFG+GPLCSAATSSSLPRFLAVGSLPIEIPDHKSPP+HSL+PP
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGRGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPHHSLLPP 120

Query: 121 SAKLVFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180
           SAKL FLILTILAINFKNH H NA+ LFYCLLIYFFLEFLIGTTA LAK VLGVELLPYF
Sbjct: 121 SAKLGFLILTILAINFKNHFHQNAVLLFYCLLIYFFLEFLIGTTAALAKKVLGVELLPYF 180

Query: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240
           NEPY SDSLQDFWGRRWNLMTSRILRL++YDPCRNLT+G+IGRR ASMVAV+ATF VSGL
Sbjct: 181 NEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRNLTVGIIGRRRASMVAVMATFGVSGL 240

Query: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300
           MHELIYFYMGRMAPTWEV+CFFVVHGVCVVAE AV+LGAGGR+RAPR  RI LTI FVMG
Sbjct: 241 MHELIYFYMGRMAPTWEVSCFFVVHGVCVVAERAVKLGAGGRYRAPRAVRIGLTIGFVMG 300

Query: 301 TGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPFNPTSFLF 349
           TGSWLFFPQCIRAKMDVRMLEEYAA+GAF K IT+ FI   +  SFLF
Sbjct: 301 TGSWLFFPQCIRAKMDVRMLEEYAAMGAFLKDITVLFI---DSISFLF 345

[Close]

BLAST of CSPI03G29390 vs. ExPASy TrEMBL
Match: A0A6J1F144 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441426 PE=3 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 6.7e-132
Identity = 241/346 (69.65%), Postives = 282/346 (81.50%), Query Frame = 0


Query: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MA+EIH+ +QTLFL++SSL LCFSIRNSIPNGFLKFL +LPFFFLFLYIPLQF TIH +G
Sbjct: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHS---- 120
            +GFFIGWLGSFKLLLFAFGKGPLC AATSSSL RFLAV  LPI+IP+HKSPPN S    
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120

Query: 121 LIPPS--AKLVFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGV 180
           L+P S  AKL FLILT  AI FKN  +PN + +F+CLLIY  LE LIG    LA+ VLGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180

Query: 181 ELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVIAT 240
           ELLP+F+EPY SDSLQDFWGRRWNL++SRILR +++DPCR L   V+G++PA+++AV+AT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240

Query: 241 FVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLT 300
           F VSG+MHELIYFYMGR+ PTWEV+ FF++HGVC+ AEMAV+   GGRFR PRV    LT
Sbjct: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300

Query: 301 IMFVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPP 341
           I FVM T  WLFFPQ +R K DVRMLEEYAA+GAF K++T   I P
Sbjct: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGP 346

[Close]

BLAST of CSPI03G29390 vs. ExPASy TrEMBL
Match: A0A6J1IX47 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481451 PE=3 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 1.1e-131
Identity = 241/346 (69.65%), Postives = 282/346 (81.50%), Query Frame = 0


Query: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MA+EIH+ +QTLFL+SSSL LCFSIRNSIPNGFLKF+ +LPFFFLFLYIPLQF TIH +G
Sbjct: 1   MAAEIHAFIQTLFLISSSLCLCFSIRNSIPNGFLKFIFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHS---- 120
            +GFFIGWLGSFKLLLFAFGKGPLCSAATSSSL RFLAV SLPI+IP+HK PPN S    
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVASLPIDIPEHKPPPNRSDPDP 120

Query: 121 LIPPS--AKLVFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGV 180
           L+P S  AKL FLILT  AI FKN  +PN + +F+CLLIY  LE LIG    +A+ VLGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATAVAEAVLGV 180

Query: 181 ELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVIAT 240
           ELLP+F+EPY SDSLQDFWGRRWNL++SRILR +++DPCR L   V+G++PA ++AV+AT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAVIIAVMAT 240

Query: 241 FVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLT 300
           F VSG+MHELIYFYMGR+APTWEV+ FF++HGVC+ AEMAV+   G RFRAPRV    LT
Sbjct: 241 FAVSGVMHELIYFYMGRLAPTWEVSWFFLIHGVCLAAEMAVKSAVGRRFRAPRVVSGVLT 300

Query: 301 IMFVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPP 341
           + FVM T  WLFFPQ +R K DVRMLEEYAA+GAF K +T   I P
Sbjct: 301 MGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKNVTAELIGP 346

[Close]

BLAST of CSPI03G29390 vs. NCBI nr
Match: XP_004149743.1 (acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus])

HSP 1 Score: 694.5 bits (1791), Expect = 4.7e-196
Identity = 346/349 (99.14%), Postives = 348/349 (99.71%), Query Frame = 0


Query: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGF KFLLILPFFFLFLYIPLQFHTIHFQG
Sbjct: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFFKFLLILPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
           PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120

Query: 121 SAKLVFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180
           SAKL+FLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF
Sbjct: 121 SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180

Query: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240
           NEPYFSDSLQDFWGRRWNLMTSRILRL+IYDPCRNLTIGVIGRRPASMVAVIATFVVSGL
Sbjct: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240

Query: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300
           MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG
Sbjct: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300

Query: 301 TGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPFNPTSFLFH 350
           TGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPFNPTSFLFH
Sbjct: 301 TGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPFNPTSFLFH 349

[Close]

BLAST of CSPI03G29390 vs. NCBI nr
Match: KAE8650923.1 (hypothetical protein Csa_001633 [Cucumis sativus])

HSP 1 Score: 645.2 bits (1663), Expect = 3.3e-181
Identity = 323/326 (99.08%), Postives = 325/326 (99.69%), Query Frame = 0


Query: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGF KFLLILPFFFLFLYIPLQFHTIHFQG
Sbjct: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFFKFLLILPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
           PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120

Query: 121 SAKLVFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180
           SAKL+FLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF
Sbjct: 121 SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180

Query: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240
           NEPYFSDSLQDFWGRRWNLMTSRILRL+IYDPCRNLTIGVIGRRPASMVAVIATFVVSGL
Sbjct: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240

Query: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300
           MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG
Sbjct: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300

Query: 301 TGSWLFFPQCIRAKMDVRMLEEYAAI 327
           TGSWLFFPQCIRAKMDVRMLEEYAAI
Sbjct: 301 TGSWLFFPQCIRAKMDVRMLEEYAAI 326

[Close]

BLAST of CSPI03G29390 vs. NCBI nr
Match: XP_008461836.1 (PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo] >KAA0048418.1 acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo var. makuwa] >TYJ97432.1 acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 612.1 bits (1577), Expect = 3.1e-171
Identity = 308/348 (88.51%), Postives = 326/348 (93.68%), Query Frame = 0


Query: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MA+EIHSLLQTLFLVSSSLS+CFSIRNSIPNGFLKFLL+LPFFFLFLYIPLQFHTIHFQG
Sbjct: 1   MAAEIHSLLQTLFLVSSSLSICFSIRNSIPNGFLKFLLVLPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
           PIGFFIGWLGSFKLLLFAFG+GPLCSAATSSSLPRFLAVGSLPIEIPDHKSPP+HSL+PP
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGRGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPHHSLLPP 120

Query: 121 SAKLVFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180
           SAKL FLILTILAINFKNH H NA+ LFYCLLIYFFLEFLIGTTA LAK VLGVELLPYF
Sbjct: 121 SAKLGFLILTILAINFKNHFHQNAVLLFYCLLIYFFLEFLIGTTAALAKKVLGVELLPYF 180

Query: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240
           NEPY SDSLQDFWGRRWNLMTSRILRL++YDPCRNLT+G+IGRR ASMVAV+ATF VSGL
Sbjct: 181 NEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRNLTVGIIGRRRASMVAVMATFGVSGL 240

Query: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300
           MHELIYFYMGRMAPTWEV+CFFVVHGVCVVAE AV+LGAGGR+RAPR  RI LTI FVMG
Sbjct: 241 MHELIYFYMGRMAPTWEVSCFFVVHGVCVVAERAVKLGAGGRYRAPRAVRIGLTIGFVMG 300

Query: 301 TGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPFNPTSFLF 349
           TGSWLFFPQCIRAKMDVRMLEEYAA+GAF K IT+ FI   +  SFLF
Sbjct: 301 TGSWLFFPQCIRAKMDVRMLEEYAAMGAFLKDITVLFI---DSISFLF 345

[Close]

BLAST of CSPI03G29390 vs. NCBI nr
Match: XP_038903196.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 578.6 bits (1490), Expect = 3.7e-161
Identity = 292/347 (84.15%), Postives = 315/347 (90.78%), Query Frame = 0


Query: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MA+EIHSLLQTLFLVSSSL  CF+IRNSIPNGFLKFLLILPFFFLFLYIPLQF TIHFQG
Sbjct: 1   MAAEIHSLLQTLFLVSSSLCFCFTIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHS---L 120
           PIGFFIGWLGSFKLLLFAFGKGPL SAATSSSLPRFLAVGSLPIEIPDH+SPPNHS   L
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGKGPLSSAATSSSLPRFLAVGSLPIEIPDHQSPPNHSNPKL 120

Query: 121 IPP---SAKLVFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGV 180
           + P   SAKL FLILT++AINFKNH HPNA  LFYCLLIYFFLEFLIG+TA +AK VLGV
Sbjct: 121 VLPFSYSAKLAFLILTMVAINFKNHFHPNAALLFYCLLIYFFLEFLIGSTAAVAKAVLGV 180

Query: 181 ELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVIAT 240
           ELLPYFNEPY SDSLQDFWGRRWNLMTSRILRL++YDPCR LTIGVIGR+PA+M+AV+AT
Sbjct: 181 ELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTIGVIGRKPATMLAVMAT 240

Query: 241 FVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLT 300
           FVVSGLMHELIYFY+GR+APTWEVTCFF+VHGVC+V EMAV+L AGGRFR P+V  I LT
Sbjct: 241 FVVSGLMHELIYFYIGRLAPTWEVTCFFLVHGVCLVTEMAVKLAAGGRFRPPQVVSIPLT 300

Query: 301 IMFVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPF 342
           I FVMGTG WLFFPQC+RAKMDVRM EEYAAIGAF K+IT+  I PF
Sbjct: 301 IGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKSITVDLITPF 347

[Close]

BLAST of CSPI03G29390 vs. NCBI nr
Match: XP_038905685.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 500.4 bits (1287), Expect = 1.3e-137
Identity = 253/348 (72.70%), Postives = 282/348 (81.03%), Query Frame = 0


Query: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MA+EI   LQTLFLVSSSL  CF+IRNSIPNGFLKFLLILPFFFLFLYIPLQF +IHFQG
Sbjct: 1   MAAEIRIFLQTLFLVSSSLCFCFTIRNSIPNGFLKFLLILPFFFLFLYIPLQFQSIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
            IGFFIGWLGSFKLLLFAFGKGPLCSAA SSSL  FLAVGSLPIEIPDHK   NHS   P
Sbjct: 61  SIGFFIGWLGSFKLLLFAFGKGPLCSAAASSSLRHFLAVGSLPIEIPDHKPAANHSHSNP 120

Query: 121 ------SAKLVFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGV 180
                 S+KL  LILTI+AI FKN  HPNAI +F+C +IY  LE L+G  A LAK  LGV
Sbjct: 121 VLPLSFSSKLGLLILTIVAIYFKNLFHPNAILIFFCFVIYLLLEILLGGAAALAKATLGV 180

Query: 181 ELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVIAT 240
           ELLPYF+EPY SDSLQDFWGRRWNLMTSRILRL +YDPCR +TIG++GR+PA+M+A+ AT
Sbjct: 181 ELLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLVVYDPCRKITIGIVGRKPAAMMAMTAT 240

Query: 241 FVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLT 300
           F VSGLMHELIYFYMGR+APTWEVTCFF++HGVC+ AEMA++   GGRFR P V    LT
Sbjct: 241 FAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMAIKSAVGGRFRLPGVVSAALT 300

Query: 301 IMFVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPFN 343
           I FVM T  WLFFPQ ++ K DVRM EEYAA+GAF K++T   I P N
Sbjct: 301 IGFVMTTSFWLFFPQFLKLKSDVRMFEEYAALGAFVKSVTGQLITPLN 348

[Close]

BLAST of CSPI03G29390 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 250.0 bits (637), Expect = 2.8e-66
Identity = 139/332 (41.87%), Postives = 192/332 (57.83%), Query Frame = 0


Query: 7   SLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQGPIGFFI 66
           S ++   LV  SL   F I   +P G  + +L  P F +F  +P   +++H  G   FFI
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  GWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIP---------DHKSPPNHSL 126
            WL +FKLLLFA G+GPL S     SLP FLAV  LPI+I           H+      L
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPL 122

Query: 127 IPPSAKLVFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELL 186
           I  + K VF++L I A  +   L    +   Y + IYF LE ++  TA   + +  +EL 
Sbjct: 123 I-YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELE 182

Query: 187 PYFNEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVIATFVV 246
           P FN+PY + SLQDFWGRRWNLM + ILR ++Y+P   L   V+G   + ++A   TFVV
Sbjct: 183 PQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQL-FSVLGPNYSQILAAFGTFVV 242

Query: 247 SGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMF 306
           SG+MHELI+FYMGR+ P W++  FF+++G C   E+A++    GR+R P+     LT+ F
Sbjct: 243 SGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTF 302

Query: 307 VMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAF 330
           VM T  WLF P+  R  +  + L+EYAAIGAF
Sbjct: 303 VMVTALWLFLPEFNRCNIVEKALDEYAAIGAF 332

[Close]

BLAST of CSPI03G29390 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 220.3 bits (560), Expect = 2.4e-57
Identity = 131/335 (39.10%), Postives = 184/335 (54.93%), Query Frame = 0


Query: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           M  E+ SL++       S+S C+     I +G  + L +LP   LFL +PL   ++HF G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
              FF+ WL +FKL+LF+F +GPL      S+L RF+     PI++  +  P N   +P 
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPL--FPVPSNLSRFVCFTCFPIKLQQNPKPQNQ--MPK 120

Query: 121 ---SAKLVFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELL 180
              + KL F  + +    +K H+ P  + + Y L IY   E L+     L    L  +L 
Sbjct: 121 WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDLE 180

Query: 181 PYFNEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVIATFVV 240
           P+FNEPY S SLQDFWGRRWNLM   ILR ++Y P R +    +    A  + V A+F+V
Sbjct: 181 PHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFLV 240

Query: 241 SGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAV-RLGAGGRFRAPRVARICLTIM 300
           SG++HELI+FY  R +PT EVT FFV+HGVC  AE A  R     R++  ++    LT+ 
Sbjct: 241 SGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLTVG 300

Query: 301 FVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFFK 332
           FV+ TG WLFFP   R+ M  R+ +E      F K
Sbjct: 301 FVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVK 331

[Close]

BLAST of CSPI03G29390 vs. TAIR 10
Match: AT5G55370.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 204.9 bits (520), Expect = 1.0e-52
Identity = 124/335 (37.01%), Postives = 186/335 (55.52%), Query Frame = 0


Query: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           M  E+ +L++       S+S C+ I + I  G L+ L +LP F +FL +PL F ++HF  
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
             GFF  WL +FKL LFAF + PL  +   S+L RF      PI+I  +K+P ++ +   
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL--SPLPSNLTRFFCFACFPIKI--NKNPSSNRIHNK 120

Query: 121 SAKLVFLILTILAINFKNHLHPN---------AIPLFYCLLIYFFLEFLIGTTATLAKTV 180
                 L   +L  +F  H++ N         A    + + +Y   E ++     L   +
Sbjct: 121 PMSKWVLAFKLLIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSML 180

Query: 181 LGVELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAV 240
           LG E+ P FN+PY + SLQ+FW RRWNLM   +LR +++ P +     ++G   A    +
Sbjct: 181 LGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAGM 240

Query: 241 IATFVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFR---APRV 300
           +ATF+VSGLMHELIYFY+ R +PTWEVTCFF++HGV    E+A++     R R    PR 
Sbjct: 241 LATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMK-----RMRWLPTPRR 300

Query: 301 ARICLTI-MFVMGTGSWLFFPQCIRAKMDVRMLEE 323
           A   L I +F++ T  WLF+PQ +R  +  R++ E
Sbjct: 301 AVSGLAITVFLLVTAGWLFYPQMLRNDVHKRVISE 326

[Close]

BLAST of CSPI03G29390 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 201.8 bits (512), Expect = 8.9e-52
Identity = 126/331 (38.07%), Postives = 177/331 (53.47%), Query Frame = 0


Query: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           M  +  +L++       SLS C+ I + +  G L+ L ILP   LFL +PL    +HF  
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSP---PNHSL 120
               F+ WL +FKLLLFAF +GPL        L RF+    LPI+I    SP   PN   
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPL--FPLPPKLSRFICFACLPIKIRQDPSPNAIPNLHP 120

Query: 121 IPPSAKLVFLILTILAINFKNHLHPNAIPLF-----YCLLIYFFLEFLIGTTATLAKTVL 180
            P    ++ + + +L +    + + + +P F     YCL IY  +E ++     +  T+L
Sbjct: 121 KPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLL 180

Query: 181 GVELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVI 240
           G  + P FNEPY + SLQDFW RRWNLM S +LR +++ P +     ++    A    V+
Sbjct: 181 GCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVM 240

Query: 241 ATFVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARIC 300
           A+F VSGLMHEL+YFYM R  PTWEVTCFFV+HG     E+AV+       R P  A   
Sbjct: 241 ASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRTQW--LRPPHRAVSG 300

Query: 301 LTIM-FVMGTGSWLFFPQCIRAKMDVRMLEE 323
           L ++ FV  TG WLF  Q +R  +  + + E
Sbjct: 301 LVVLTFVSVTGVWLFLAQVLRNNVHEKAIGE 327

[Close]

BLAST of CSPI03G29390 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 201.4 bits (511), Expect = 1.2e-51
Identity = 130/340 (38.24%), Postives = 189/340 (55.59%), Query Frame = 0


Query: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           M  E+ +L++       S+S C+ I   I +G  +FL + P   LFL +PL F ++H   
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
              FF+ WL +FKL+LF+F KGPL    T  + PRF      PI++  +    NH  +P 
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPIPT--NFPRFFCFTCFPIKVQQNPKSQNH--LP- 120

Query: 121 SAKLVFLI-LTILAI-----NFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGV 180
             KLVF I L I A+     +++ +L P  +   Y + +Y  +E ++     +    LG 
Sbjct: 121 --KLVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLGC 180

Query: 181 ELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLSIYDPCRNLTIGVIGRRPASMVAVIAT 240
           +L P  N+PY + SLQDFWGRRWNLM   ILR ++Y P R ++   +    A    ++A 
Sbjct: 181 DLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAA 240

Query: 241 FVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAV--RLGAGGRFR-APRVARI 300
           F+VSGL+HEL++FY+ R  PT EVT FFV+HGVC   E+AV  +     R+R +P V+R+
Sbjct: 241 FIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSPGVSRV 300

Query: 301 CLTIMFVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFFK 332
            LT+ FV  TG WLF PQ  R+ +  R   E   +  F K
Sbjct: 301 -LTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIK 332

The following BLAST results are available for this feature:

BLAST of CSPI03G29390 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	4.0e-65	41.87	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9XGY6	3.1e-57	41.25	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9FJ75	3.4e-56	39.10	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9FJ73	1.5e-51	37.01	Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX...	[more]
Q9FJ72	1.2e-50	38.07	Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX...	[more]

BLAST of CSPI03G29390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9R8	2.3e-196	99.14	MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G683670 PE=...	[more]
A0A5D3BC71	1.5e-171	88.51	Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3CG36	1.5e-171	88.51	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC10350034...	[more]
A0A6J1F144	6.7e-132	69.65	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1IX47	1.1e-131	69.65	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of CSPI03G29390 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149743.1	4.7e-196	99.14	acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus]	[more]
KAE8650923.1	3.3e-181	99.08	hypothetical protein Csa_001633 [Cucumis sativus]	[more]
XP_008461836.1	3.1e-171	88.51	PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo] >KAA0048418....	[more]
XP_038903196.1	3.7e-161	84.15	acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida]	[more]
XP_038905685.1	1.3e-137	72.70	acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida]	[more]

BLAST of CSPI03G29390 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	2.8e-66	41.87	acyl-CoA sterol acyl transferase 1	[more]
AT5G55350.1	2.4e-57	39.10	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55370.1	1.0e-52	37.01	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	8.9e-52	38.07	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55340.1	1.2e-51	38.24	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 178..263 e-value: 2.4E-16 score: 59.8
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 3..334
None	No IPR available	PANTHER	PTHR31595:SF46	ACYL-COA--STEROL O-ACYLTRANSFERASE 1	coord: 3..334

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G29390.1	CSPI03G29390.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity