CSPI03G28400 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G28400
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	non-specific lipid transfer protein GPI-anchored 1
Location	Chr3: 26006792 .. 26007323 (+)
RNA-Seq Expression	CSPI03G28400
Synteny	CSPI03G28400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAAACGTTGTTCATCTCCAAATGGGTTCATTAATTTGGAGATCATAGCAATCGAGAAAGAGAAAAAAATGAAGAGTGGAAGAAAGGTGATGAGATCAGGTGTGGTTTTAGCGGTGGTGGCGGTGTGTTTATTAGGAAGGGTGGTGTCAGGACGAGCACAGGAAGATAATTTGAAAGAGGTGTGCAGCAATGACTTCGAGAAAGTGGTGAGTTGTTTCGCGTATGCGACGGGGAAAGCAATGACACCGATGGAGGAGTGTTGTGATTCGATTGAAGGTATAAAAGAAAGTAAGCCAAAGTGCTTGTGTTTCTTCATACAACAGACACATAATGGAAATCAACACATCAAGAGCTTAGGAATTCAAGAGATTAAGCTTTTACATCTTCCTTCTGTTTGTCACTTCAAAAGCTGGAGTGTTAGCCATTCCCTAACTAATTTCTCTTCCCATCCCTCTTTCTTTCTTTTCTTTTCTTTTCTTTTTTCTTAAATTTAATTTATATTTTCTAGGTTGCTGGATTGTTCAGTAA

mRNA sequence

ATGGAAAAACGTTGTTCATCTCCAAATGGGTTCATTAATTTGGAGATCATAGCAATCGAGAAAGAGAAAAAAATGAAGAGTGGAAGAAAGGTGATGAGATCAGGTGTGGTTTTAGCGGTGGTGGCGGTGTGTTTATTAGGAAGGGTGGTGTCAGGACGAGCACAGGAAGATAATTTGAAAGAGGTGTGCAGCAATGACTTCGAGAAAGTGGTGAGTTGTTTCGCGTATGCGACGGGGAAAGCAATGACACCGATGGAGGAGTGTTGTGATTCGATTGAAGGTTGCTGGATTGTTCAGTAA

Coding sequence (CDS)

ATGGAAAAACGTTGTTCATCTCCAAATGGGTTCATTAATTTGGAGATCATAGCAATCGAGAAAGAGAAAAAAATGAAGAGTGGAAGAAAGGTGATGAGATCAGGTGTGGTTTTAGCGGTGGTGGCGGTGTGTTTATTAGGAAGGGTGGTGTCAGGACGAGCACAGGAAGATAATTTGAAAGAGGTGTGCAGCAATGACTTCGAGAAAGTGGTGAGTTGTTTCGCGTATGCGACGGGGAAAGCAATGACACCGATGGAGGAGTGTTGTGATTCGATTGAAGGTTGCTGGATTGTTCAGTAA

Protein sequence

MEKRCSSPNGFINLEIIAIEKEKKMKSGRKVMRSGVVLAVVAVCLLGRVVSGRAQEDNLKEVCSNDFEKVVSCFAYATGKAMTPMEECCDSIEGCWIVQ*

Homology

[Close]

BLAST of CSPI03G28400 vs. ExPASy Swiss-Prot
Match: Q9C7F7 (Non-specific lipid transfer protein GPI-anchored 1 OS=Arabidopsis thaliana OX=3702 GN=LTPG1 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.1e-04
Identity = 17/35 (48.57%), Postives = 25/35 (71.43%), Query Frame = 0


Query: 59 LKEVCSNDFEKVVSCFAYATGKAMTPMEECCDSIE 94
          L + C+ DF+KV  C  +ATGKA  P ++CCD++E
Sbjct: 31 LADECNQDFQKVTLCLDFATGKATIPSKKCCDAVE 65

[Close]

BLAST of CSPI03G28400 vs. ExPASy TrEMBL
Match: A0A1S3BER8 (non-specific lipid transfer protein GPI-anchored 1 OS=Cucumis melo OX=3656 GN=LOC103488880 PE=4 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.2e-25
Identity = 63/71 (88.73%), Postives = 65/71 (91.55%), Query Frame = 0


Query: 25 MKSGRKVMRS-GVVLAVVAVCLLGRVVSGRAQEDNLKEVCSNDFEKVVSCFAYATGKAMT 84
          MKSGRKVMRS GVVLAVVAVCLLGRVV GRA+E+NLKE CSNDFEKVVSCFAYATGKA  
Sbjct: 1  MKSGRKVMRSAGVVLAVVAVCLLGRVVPGRAEEENLKEECSNDFEKVVSCFAYATGKAAA 60

Query: 85 PMEECCDSIEG 95
          P EECCDSIEG
Sbjct: 61 PTEECCDSIEG 71

[Close]

BLAST of CSPI03G28400 vs. ExPASy TrEMBL
Match: A0A0A0KV96 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G589340 PE=4 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.2e-22
Identity = 59/69 (85.51%), Postives = 60/69 (86.96%), Query Frame = 0


Query: 25 MKSGRKVMRSGVVLAVVAVCLLGRVVSGRAQEDNLKEVCSNDFEKVVSCFAYATGKAMTP 84
          MKSGRKV    VVLAVVAVCLLGRVV GRAQED+LKE CSNDFEKVVSCFAYATGKA  P
Sbjct: 1  MKSGRKV---SVVLAVVAVCLLGRVVPGRAQEDDLKEECSNDFEKVVSCFAYATGKAAAP 60

Query: 85 MEECCDSIE 94
           EECCDSIE
Sbjct: 61 TEECCDSIE 66

[Close]

BLAST of CSPI03G28400 vs. ExPASy TrEMBL
Match: A0A5A7SYJ3 (Non-specific lipid transfer protein GPI-anchored 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G001840 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 8.0e-22
Identity = 56/64 (87.50%), Postives = 58/64 (90.62%), Query Frame = 0


Query: 32 MRS-GVVLAVVAVCLLGRVVSGRAQEDNLKEVCSNDFEKVVSCFAYATGKAMTPMEECCD 91
          MRS GVVLAVVAVCLLGRVV GRA+E+NLKE CSNDFEKVVSCFAYATGKA  P EECCD
Sbjct: 1  MRSAGVVLAVVAVCLLGRVVPGRAEEENLKEECSNDFEKVVSCFAYATGKAAAPTEECCD 60

Query: 92 SIEG 95
          SIEG
Sbjct: 61 SIEG 64

[Close]

BLAST of CSPI03G28400 vs. ExPASy TrEMBL
Match: A0A6J1GX01 (non-specific lipid transfer protein GPI-anchored 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458210 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 5.8e-12
Identity = 36/58 (62.07%), Postives = 45/58 (77.59%), Query Frame = 0


Query: 37 VLAVVAVCLLGRVVSGRAQEDNLKEVCSNDFEKVVSCFAYATGKAMTPMEECCDSIEG 95
          V+ VV V L+G +    A+E+NLKE CSNDF++VVSCF +ATGKA  P +ECCDSIEG
Sbjct: 7  VVVVVVVVLVGLLCLAGAEEENLKEECSNDFQQVVSCFEFATGKAAAPSKECCDSIEG 64

[Close]

BLAST of CSPI03G28400 vs. ExPASy TrEMBL
Match: A0A6J1K8M9 (non-specific lipid transfer protein GPI-anchored 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493265 PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.4e-10
Identity = 38/69 (55.07%), Postives = 50/69 (72.46%), Query Frame = 0


Query: 25 MKSGRKVMRSGVVLAVVAVCLLGRVVSGRAQEDNLKEVCSNDFEKVVSCFAYATGKAMTP 84
          MK G  V+   ++L +V +CL+G      A+E+NLKE CSNDF++VVSCF +ATGKA  P
Sbjct: 2  MKGGNGVV--VLLLLLVGLCLVG------AEEENLKEECSNDFQQVVSCFEFATGKAAAP 61

Query: 85 MEECCDSIE 94
           +ECCDSIE
Sbjct: 62 SKECCDSIE 62

[Close]

BLAST of CSPI03G28400 vs. NCBI nr
Match: XP_008446028.1 (PREDICTED: non-specific lipid transfer protein GPI-anchored 1 [Cucumis melo])

HSP 1 Score: 125.6 bits (314), Expect = 2.5e-25
Identity = 63/71 (88.73%), Postives = 65/71 (91.55%), Query Frame = 0


Query: 25 MKSGRKVMRS-GVVLAVVAVCLLGRVVSGRAQEDNLKEVCSNDFEKVVSCFAYATGKAMT 84
          MKSGRKVMRS GVVLAVVAVCLLGRVV GRA+E+NLKE CSNDFEKVVSCFAYATGKA  
Sbjct: 1  MKSGRKVMRSAGVVLAVVAVCLLGRVVPGRAEEENLKEECSNDFEKVVSCFAYATGKAAA 60

Query: 85 PMEECCDSIEG 95
          P EECCDSIEG
Sbjct: 61 PTEECCDSIEG 71

[Close]

BLAST of CSPI03G28400 vs. NCBI nr
Match: XP_004135333.3 (non-specific lipid transfer protein GPI-anchored 1 [Cucumis sativus] >KGN52285.2 hypothetical protein Csa_008344 [Cucumis sativus])

HSP 1 Score: 117.9 bits (294), Expect = 5.2e-23
Identity = 60/70 (85.71%), Postives = 61/70 (87.14%), Query Frame = 0


Query: 25 MKSGRKVMRSGVVLAVVAVCLLGRVVSGRAQEDNLKEVCSNDFEKVVSCFAYATGKAMTP 84
          MKSGRKV    VVLAVVAVCLLGRVV GRAQED+LKE CSNDFEKVVSCFAYATGKA  P
Sbjct: 1  MKSGRKV---SVVLAVVAVCLLGRVVPGRAQEDDLKEECSNDFEKVVSCFAYATGKAAAP 60

Query: 85 MEECCDSIEG 95
           EECCDSIEG
Sbjct: 61 TEECCDSIEG 67

[Close]

BLAST of CSPI03G28400 vs. NCBI nr
Match: KAE8650882.1 (hypothetical protein Csa_000781 [Cucumis sativus])

HSP 1 Score: 117.9 bits (294), Expect = 5.2e-23
Identity = 60/61 (98.36%), Postives = 60/61 (98.36%), Query Frame = 0


Query: 1  MEKRCSSPNGFINLEIIAIEKEKKMKSGRKVMRSGVVLAVVAVCLLGRVVSGRAQEDNLK 60
          MEKRCSSPNGFINLEIIAIEKEKKMKSGRKVMRSGVVLAVVAVCLLGRVV GRAQEDNLK
Sbjct: 1  MEKRCSSPNGFINLEIIAIEKEKKMKSGRKVMRSGVVLAVVAVCLLGRVVPGRAQEDNLK 60

Query: 61 E 62
          E
Sbjct: 61 E 61

[Close]

BLAST of CSPI03G28400 vs. NCBI nr
Match: KAA0034189.1 (non-specific lipid transfer protein GPI-anchored 1 [Cucumis melo var. makuwa])

HSP 1 Score: 112.8 bits (281), Expect = 1.7e-21
Identity = 56/64 (87.50%), Postives = 58/64 (90.62%), Query Frame = 0


Query: 32 MRS-GVVLAVVAVCLLGRVVSGRAQEDNLKEVCSNDFEKVVSCFAYATGKAMTPMEECCD 91
          MRS GVVLAVVAVCLLGRVV GRA+E+NLKE CSNDFEKVVSCFAYATGKA  P EECCD
Sbjct: 1  MRSAGVVLAVVAVCLLGRVVPGRAEEENLKEECSNDFEKVVSCFAYATGKAAAPTEECCD 60

Query: 92 SIEG 95
          SIEG
Sbjct: 61 SIEG 64

[Close]

BLAST of CSPI03G28400 vs. NCBI nr
Match: XP_038891858.1 (non-specific lipid transfer protein GPI-anchored 1 [Benincasa hispida])

HSP 1 Score: 89.0 bits (219), Expect = 2.6e-14
Identity = 42/72 (58.33%), Postives = 55/72 (76.39%), Query Frame = 0


Query: 23 KKMKSGRKVMRSGVVLAVVAVCLLGRVVSGRAQEDNLKEVCSNDFEKVVSCFAYATGKAM 82
          +KMK G + + + +++AV  VCL+GR     A++D+LKE CS+DFEKVVSCF YATGKA 
Sbjct: 2  RKMKGGIRKVGAALLVAVAMVCLMGR-PGKAAEDDHLKEECSSDFEKVVSCFEYATGKAA 61

Query: 83 TPMEECCDSIEG 95
           P +ECCDSIEG
Sbjct: 62 APSKECCDSIEG 72

[Close]

BLAST of CSPI03G28400 vs. TAIR 10
Match: AT1G27950.1 (glycosylphosphatidylinositol-anchored lipid protein transfer 1 )

HSP 1 Score: 47.4 bits (111), Expect = 8.0e-06
Identity = 17/35 (48.57%), Postives = 25/35 (71.43%), Query Frame = 0


Query: 59 LKEVCSNDFEKVVSCFAYATGKAMTPMEECCDSIE 94
          L + C+ DF+KV  C  +ATGKA  P ++CCD++E
Sbjct: 31 LADECNQDFQKVTLCLDFATGKATIPSKKCCDAVE 65

The following BLAST results are available for this feature:

BLAST of CSPI03G28400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C7F7	1.1e-04	48.57	Non-specific lipid transfer protein GPI-anchored 1 OS=Arabidopsis thaliana OX=37...	[more]

BLAST of CSPI03G28400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BER8	1.2e-25	88.73	non-specific lipid transfer protein GPI-anchored 1 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A0A0KV96	1.2e-22	85.51	AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G589340 PE=4 SV...	[more]
A0A5A7SYJ3	8.0e-22	87.50	Non-specific lipid transfer protein GPI-anchored 1 OS=Cucumis melo var. makuwa O...	[more]
A0A6J1GX01	5.8e-12	62.07	non-specific lipid transfer protein GPI-anchored 1-like OS=Cucurbita moschata OX...	[more]
A0A6J1K8M9	1.4e-10	55.07	non-specific lipid transfer protein GPI-anchored 1-like OS=Cucurbita maxima OX=3...	[more]

BLAST of CSPI03G28400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008446028.1	2.5e-25	88.73	PREDICTED: non-specific lipid transfer protein GPI-anchored 1 [Cucumis melo]	[more]
XP_004135333.3	5.2e-23	85.71	non-specific lipid transfer protein GPI-anchored 1 [Cucumis sativus] >KGN52285.2...	[more]
KAE8650882.1	5.2e-23	98.36	hypothetical protein Csa_000781 [Cucumis sativus]	[more]
KAA0034189.1	1.7e-21	87.50	non-specific lipid transfer protein GPI-anchored 1 [Cucumis melo var. makuwa]	[more]
XP_038891858.1	2.6e-14	58.33	non-specific lipid transfer protein GPI-anchored 1 [Benincasa hispida]	[more]

BLAST of CSPI03G28400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G27950.1	8.0e-06	48.57	glycosylphosphatidylinositol-anchored lipid protein transfer 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 63..94

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G28400.1	CSPI03G28400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042335	cuticle development
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0006869	lipid transport
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009505	plant-type cell wall
molecular_function	GO:0008289	lipid binding