CSPI03G25280 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G25280
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	PMEI domain-containing protein
Location	Chr3: 22531943 .. 22532233 (+)
RNA-Seq Expression	CSPI03G25280
Synteny	CSPI03G25280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCTCAAGCAGGGGTGTTGGTGCGATAGCAAAGGCAAAAAAAGTGGCAGGACTCCTGGGGAGGTTGACGAACAACAAGAGGATGAAGGGGAGAAACAAGGCAATCCAAGCTGTGTGGAGGTGTTTTGGAGCCGTCATTGATGAATTTCACAGGCAGAGAAATTTTGACGCACAAATGGGGGATCTTACGACGTGGGTCAGAGCTATGCTAATGGATGAAGATACTTGCATCAGACGATTTAAGGGTGAAAGAGGAAAAGTAGTGAATTTTCTAAGGAATCAAGGGTGA

mRNA sequence

ATGGGCTCAAGCAGGGGTGTTGGTGCGATAGCAAAGGCAAAAAAAGTGGCAGGACTCCTGGGGAGGTTGACGAACAACAAGAGGATGAAGGGGAGAAACAAGGCAATCCAAGCTGTGTGGAGGTGTTTTGGAGCCGTCATTGATGAATTTCACAGGCAGAGAAATTTTGACGCACAAATGGGGGATCTTACGACGTGGGTCAGAGCTATGCTAATGGATGAAGATACTTGCATCAGACGATTTAAGGGTGAAAGAGGAAAAGTAGTGAATTTTCTAAGGAATCAAGGGTGA

Coding sequence (CDS)

ATGGGCTCAAGCAGGGGTGTTGGTGCGATAGCAAAGGCAAAAAAAGTGGCAGGACTCCTGGGGAGGTTGACGAACAACAAGAGGATGAAGGGGAGAAACAAGGCAATCCAAGCTGTGTGGAGGTGTTTTGGAGCCGTCATTGATGAATTTCACAGGCAGAGAAATTTTGACGCACAAATGGGGGATCTTACGACGTGGGTCAGAGCTATGCTAATGGATGAAGATACTTGCATCAGACGATTTAAGGGTGAAAGAGGAAAAGTAGTGAATTTTCTAAGGAATCAAGGGTGA

Protein sequence

MGSSRGVGAIAKAKKVAGLLGRLTNNKRMKGRNKAIQAVWRCFGAVIDEFHRQRNFDAQMGDLTTWVRAMLMDEDTCIRRFKGERGKVVNFLRNQG*

Homology

[Close]

BLAST of CSPI03G25280 vs. ExPASy TrEMBL
Match: A0A0A0LDI5 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G560260 PE=4 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 8.3e-48
Identity = 96/96 (100.00%), Postives = 96/96 (100.00%), Query Frame = 0


Query: 1  MGSSRGVGAIAKAKKVAGLLGRLTNNKRMKGRNKAIQAVWRCFGAVIDEFHRQRNFDAQM 60
          MGSSRGVGAIAKAKKVAGLLGRLTNNKRMKGRNKAIQAVWRCFGAVIDEFHRQRNFDAQM
Sbjct: 1  MGSSRGVGAIAKAKKVAGLLGRLTNNKRMKGRNKAIQAVWRCFGAVIDEFHRQRNFDAQM 60

Query: 61 GDLTTWVRAMLMDEDTCIRRFKGERGKVVNFLRNQG 97
          GDLTTWVRAMLMDEDTCIRRFKGERGKVVNFLRNQG
Sbjct: 61 GDLTTWVRAMLMDEDTCIRRFKGERGKVVNFLRNQG 96

[Close]

BLAST of CSPI03G25280 vs. ExPASy TrEMBL
Match: A0A0A0KAX0 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G397050 PE=4 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 2.1e-22
Identity = 63/98 (64.29%), Postives = 69/98 (70.41%), Query Frame = 0


Query: 10  IAKAKKVAGLLGRLTNNKRMKGRNKAIQAVWRC---FGAVIDEFHR---------QRNFD 69
           I +AKKVAGLLGRL NNKRMKGRN+A  AV  C   F A IDE HR         +RNFD
Sbjct: 73  ITEAKKVAGLLGRLKNNKRMKGRNRA--AVLDCVEVFEAAIDELHRSLGVLRRLSRRNFD 132

Query: 70  AQMGDLTTWVRAMLMDEDTCIRRFKGERGKVVNFLRNQ 96
           AQMGDLTTWV A L DEDTC+  F+GE GKVV  LRN+
Sbjct: 133 AQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNR 168

[Close]

BLAST of CSPI03G25280 vs. ExPASy TrEMBL
Match: A0A5D3D1R1 (21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00630 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 3.5e-22
Identity = 62/98 (63.27%), Postives = 70/98 (71.43%), Query Frame = 0


Query: 10  IAKAKKVAGLLGRLTNNKRMKGRNKAIQAVWRC---FGAVIDEFHR---------QRNFD 69
           I +AKKVA LLGRL NNKRMKGRN+A  AV  C   F A IDE HR         +RNFD
Sbjct: 73  ITEAKKVAALLGRLKNNKRMKGRNRA--AVLDCVEVFDAAIDELHRSLGVLRRLSRRNFD 132

Query: 70  AQMGDLTTWVRAMLMDEDTCIRRFKGERGKVVNFLRNQ 96
           +QMGDLTTWV A L DEDTC+  F+GERGKVV+ LRN+
Sbjct: 133 SQMGDLTTWVSAALTDEDTCVEGFEGERGKVVSLLRNR 168

[Close]

BLAST of CSPI03G25280 vs. ExPASy TrEMBL
Match: A0A1S3BSW5 (21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492810 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 3.5e-22
Identity = 62/98 (63.27%), Postives = 70/98 (71.43%), Query Frame = 0


Query: 10  IAKAKKVAGLLGRLTNNKRMKGRNKAIQAVWRC---FGAVIDEFHR---------QRNFD 69
           I +AKKVA LLGRL NNKRMKGRN+A  AV  C   F A IDE HR         +RNFD
Sbjct: 73  ITEAKKVAALLGRLKNNKRMKGRNRA--AVLDCVEVFDAAIDELHRSLGVLRRLSRRNFD 132

Query: 70  AQMGDLTTWVRAMLMDEDTCIRRFKGERGKVVNFLRNQ 96
           +QMGDLTTWV A L DEDTC+  F+GERGKVV+ LRN+
Sbjct: 133 SQMGDLTTWVSAALTDEDTCVEGFEGERGKVVSLLRNR 168

[Close]

BLAST of CSPI03G25280 vs. ExPASy TrEMBL
Match: A0A6J1L076 (pectinesterase inhibitor 6 OS=Cucurbita maxima OX=3661 GN=LOC111497889 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 7.6e-17
Identity = 54/98 (55.10%), Postives = 64/98 (65.31%), Query Frame = 0


Query: 10  IAKAKKVAGLLGRLTNNKRMKGRNKAIQAVWRC---FGAVIDEFHR---------QRNFD 69
           I +AK VA  L RL NN+RMKGRN+A  AV  C   F   IDE HR         +  FD
Sbjct: 73  ITEAKAVARFLVRLKNNRRMKGRNRA--AVSDCVEVFEDAIDELHRSLGVLRRLSRSKFD 132

Query: 70  AQMGDLTTWVRAMLMDEDTCIRRFKGERGKVVNFLRNQ 96
           AQMGDLTTWV A L DEDTC+  F+GERG++VN +RN+
Sbjct: 133 AQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNR 168

[Close]

BLAST of CSPI03G25280 vs. NCBI nr
Match: KAE8650765.1 (hypothetical protein Csa_017530 [Cucumis sativus])

HSP 1 Score: 190.7 bits (483), Expect = 6.1e-45
Identity = 92/94 (97.87%), Postives = 92/94 (97.87%), Query Frame = 0


Query: 3   SSRGVGAIAKAKKVAGLLGRLTNNKRMKGRNKAIQAVWRCFGAVIDEFHRQRNFDAQMGD 62
           S  GVGAIAKAKKVAGLLGRLTNNKRMKGRNKAIQAVWRCFGAVIDEFHRQRNFDAQMGD
Sbjct: 33  SHGGVGAIAKAKKVAGLLGRLTNNKRMKGRNKAIQAVWRCFGAVIDEFHRQRNFDAQMGD 92

Query: 63  LTTWVRAMLMDEDTCIRRFKGERGKVVNFLRNQG 97
           LTTWVRAMLMDEDTCIRRFKGERGKVVNFLRNQG
Sbjct: 93  LTTWVRAMLMDEDTCIRRFKGERGKVVNFLRNQG 126

[Close]

BLAST of CSPI03G25280 vs. NCBI nr
Match: KAE8646342.1 (hypothetical protein Csa_016554 [Cucumis sativus])

HSP 1 Score: 114.8 bits (286), Expect = 4.2e-22
Identity = 63/98 (64.29%), Postives = 69/98 (70.41%), Query Frame = 0


Query: 10  IAKAKKVAGLLGRLTNNKRMKGRNKAIQAVWRC---FGAVIDEFHR---------QRNFD 69
           I +AKKVAGLLGRL NNKRMKGRN+A  AV  C   F A IDE HR         +RNFD
Sbjct: 73  ITEAKKVAGLLGRLKNNKRMKGRNRA--AVLDCVEVFEAAIDELHRSLGVLRRLSRRNFD 132

Query: 70  AQMGDLTTWVRAMLMDEDTCIRRFKGERGKVVNFLRNQ 96
           AQMGDLTTWV A L DEDTC+  F+GE GKVV  LRN+
Sbjct: 133 AQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNR 168

[Close]

BLAST of CSPI03G25280 vs. NCBI nr
Match: XP_011659361.1 (pectinesterase inhibitor 6 [Cucumis sativus])

HSP 1 Score: 114.8 bits (286), Expect = 4.2e-22
Identity = 63/98 (64.29%), Postives = 69/98 (70.41%), Query Frame = 0


Query: 10  IAKAKKVAGLLGRLTNNKRMKGRNKAIQAVWRC---FGAVIDEFHR---------QRNFD 69
           I +AKKVAGLLGRL NNKRMKGRN+A  AV  C   F A IDE HR         +RNFD
Sbjct: 73  ITEAKKVAGLLGRLKNNKRMKGRNRA--AVLDCVEVFEAAIDELHRSLGVLRRLSRRNFD 132

Query: 70  AQMGDLTTWVRAMLMDEDTCIRRFKGERGKVVNFLRNQ 96
           AQMGDLTTWV A L DEDTC+  F+GE GKVV  LRN+
Sbjct: 133 AQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNR 168

[Close]

BLAST of CSPI03G25280 vs. NCBI nr
Match: XP_008451567.1 (PREDICTED: 21 kDa protein [Cucumis melo] >KAA0067994.1 21 kDa protein [Cucumis melo var. makuwa] >TYK18133.1 21 kDa protein [Cucumis melo var. makuwa])

HSP 1 Score: 114.0 bits (284), Expect = 7.2e-22
Identity = 62/98 (63.27%), Postives = 70/98 (71.43%), Query Frame = 0


Query: 10  IAKAKKVAGLLGRLTNNKRMKGRNKAIQAVWRC---FGAVIDEFHR---------QRNFD 69
           I +AKKVA LLGRL NNKRMKGRN+A  AV  C   F A IDE HR         +RNFD
Sbjct: 73  ITEAKKVAALLGRLKNNKRMKGRNRA--AVLDCVEVFDAAIDELHRSLGVLRRLSRRNFD 132

Query: 70  AQMGDLTTWVRAMLMDEDTCIRRFKGERGKVVNFLRNQ 96
           +QMGDLTTWV A L DEDTC+  F+GERGKVV+ LRN+
Sbjct: 133 SQMGDLTTWVSAALTDEDTCVEGFEGERGKVVSLLRNR 168

[Close]

BLAST of CSPI03G25280 vs. NCBI nr
Match: XP_038896715.1 (pectinesterase inhibitor 6-like [Benincasa hispida])

HSP 1 Score: 110.5 bits (275), Expect = 8.0e-21
Identity = 60/98 (61.22%), Postives = 69/98 (70.41%), Query Frame = 0


Query: 10  IAKAKKVAGLLGRLTNNKRMKGRNKAIQAVWRC---FGAVIDEFHR---------QRNFD 69
           I +AK VAGLLGRL NNKRMKGRN+A  AV  C   F A IDE HR         + NFD
Sbjct: 73  ITEAKTVAGLLGRLKNNKRMKGRNRA--AVSDCVEVFEAAIDELHRSLGVLRRLSRSNFD 132

Query: 70  AQMGDLTTWVRAMLMDEDTCIRRFKGERGKVVNFLRNQ 96
           AQ+GDLTTWV A L DEDTC+  F+GERGK+VN +RN+
Sbjct: 133 AQIGDLTTWVSAALTDEDTCVEGFEGERGKLVNLIRNR 168

The following BLAST results are available for this feature:

BLAST of CSPI03G25280 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI03G25280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LDI5	8.3e-48	100.00	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G560260 PE=4 S...	[more]
A0A0A0KAX0	2.1e-22	64.29	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G397050 PE=4 S...	[more]
A0A5D3D1R1	3.5e-22	63.27	21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00630...	[more]
A0A1S3BSW5	3.5e-22	63.27	21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492810 PE=4 SV=1	[more]
A0A6J1L076	7.6e-17	55.10	pectinesterase inhibitor 6 OS=Cucurbita maxima OX=3661 GN=LOC111497889 PE=4 SV=1	[more]

BLAST of CSPI03G25280 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8650765.1	6.1e-45	97.87	hypothetical protein Csa_017530 [Cucumis sativus]	[more]
KAE8646342.1	4.2e-22	64.29	hypothetical protein Csa_016554 [Cucumis sativus]	[more]
XP_011659361.1	4.2e-22	64.29	pectinesterase inhibitor 6 [Cucumis sativus]	[more]
XP_008451567.1	7.2e-22	63.27	PREDICTED: 21 kDa protein [Cucumis melo] >KAA0067994.1 21 kDa protein [Cucumis m...	[more]
XP_038896715.1	8.0e-21	61.22	pectinesterase inhibitor 6-like [Benincasa hispida]	[more]

BLAST of CSPI03G25280 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 9..87 e-value: 1.1E-6 score: 29.1
None	No IPR available	PANTHER	PTHR31080:SF158	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 9..95
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 9..95
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 10..93

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G25280.1	CSPI03G25280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity