CSPI03G25040 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G25040
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Pectinesterase
Location	Chr3: 22258909 .. 22261377 (+)
RNA-Seq Expression	CSPI03G25040
Synteny	CSPI03G25040

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACATTATTATTCGAAAGAGATAAGTTTGTTGATTATAACGACGTTGGTGGTGTTGTTTTGTGTGAAAGTTTCCAAGGCATTGGATGATTGTGAATTGAATTATAATGATCCTCATAAAGTTGCATACACTATTGTTGTTGATAAACTTGGGAAAGGTAACTTCAACACAATCCAAAGTGCCATTAATTCTGTCCCTTCCCAAAGTACCAAATGGATTAGAATTCAAGTTTCTCCTGGCATATATACGTACGGTATACGATCTTATTTCCCGTTTATTAAAATATCATTTTAGTTCCCTTGTTTGATTTCATTTTATTTTCATCAAGTTTAGATTTAGTTTTTCTTTTTCTTTTTTCCATACCTACTAAAGTCTATCACTGTTTGATTTCTATCAGCATTTATTAGTGATAGGTGATAGTTATAGAAACATGAATAGTCTAATTTGGTTAGCATGTTTATGTCGAACATTCGTTTAGTGTAACAAATGAATTAGACATGCATAGAACCTTTATATTGAGGTATATACTAAAAGAGACACTACGTGACAATAATAACTTTTGAGCATGTGATACATCAAGCAAAGTTTTTTAAGCATATAAATGTATCAATGCATATGTTATGAAGTTTCTTTTACTATAAGAATGATATTATATTTTTAAGAAAGAATATTTTAATAAATGTGTAATCATCATGTCGTGTCCTAGATTTTTAAATAATGGCGTGTTGTGGTGTCCCTATTGTGCCATATCCATGTCTCAAATTTGAATTTGTGCTATTAGGATCGGATGACAGTAGTCTTGTTTATCGTCTATCAATAATATTTTGTTATATATACTTGGAATCAATTAAAACAACCATTACACATGTTATATTTTAACAGGGAAAAAGTGACGATACCCTCCGAAAAATCATGCATATTTCTAGATGGAAGTGGGCTTCAAGTGACTGAAATTCATTGGAATGACCATGAAACAACAGCTGCTAGTCCCACCTTTACTGCTTCTGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGGTATATACATATAACTGTTTAATTTCCCTAACTTTCAAATAATGTATATATTTTCTTTGTTTTATGTCTATTATCTGTGATCCAAAATTGGCAGAATACTTATAATGCACGTGGAAGTGTGATGAGACGTGAAGATATAAAACCAGCACTTGCAGCTCTAATCCAAGGAGACAAAGTGATATTTCATAAATGTGGTTTCATTGGTTTGCAAGATACTTTGTGGGATGGTCCTGGCCGTCATCTATTCACCCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTTGGTCAGTCTATCTATAAGGTTAACTTCTAAATTAATTATAAGCTTACATGTGTGTTTGTATATGTGTGAGAGCTTGGAAGTGAAATTGAAGTGTTGAGTTTTTCTTTTCTGTTTGTTTGTTTGTTGTGTTTAACGCAGGAATGTGTGATAAACATACCAGTCAATGCATATGCTCCATTACTAAATGAAGGTTTCATAACAGCTCAAGGAAAGGAGAATCCAAATGAAAGCAGTGGATTTGTATTCCTTAGATGCATAGTGCAGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTAACCCTGCTGGTTGGAATTCTTGGCTACAGGCTGGGCATGAGTAATAATTAACTCTTCTTTTCATCTTTTTCATTTCATTTTATATAGCATATTATTAAAGTATCATTATTATCATTATTATCATTATTATCATTATTTGTTTGTAGGAGTGATTTGACGTATTCAGAGACCCGTTGTACTGGGCCAGGAGCAGATACTTCAAGCCGTGTGCCTTGGGTTAATAGATTGGATGCATTTCACATAAGAAGCTTCACAGATATTTCTTATATTGATCCACAAGGTTATTGGACTTCAAGGATTCCTCTTTTATCCTAATTAATTTAATTAAGTTTCTAAACTTAATTAATGGGCCCTTTGTAATTCTTTTCTATTCTCTCAGTAAATAATGAAAAATACTTTTCTTTTTTCAGTCATTTTAATCTTTCCTTCTACTGAAGCTTCAAATTGACTTTTTTTTTTTTCTTGCTTAGAGATTGCTAATATAACATGTAGGGTTTAAGTTAGAAAGATTTAAAAAACTAGTTGTTGGAGGGGAGCGCCAAGGCCCAAGCAGTAGTGCAACGCAAGGGCGACGCTCGAAGGCCCCAATAGCAAGCTATTGTGATGGGCCCTCCCCCTAGAAAAATAAATTTAATATCATGTGAGCCGATGGCCCACGTATCAGATATTAAACGGATAAGAACAGATACTACACTTGATCTTAGCCAAAAGGCCGAGAAAGGTATGCTTTTAGAGTCCAATGAAACTCATGTTTATACTCCGCCGCTTGCTTCCATTTTGTGA

mRNA sequence

ATGGAACATTATTATTCGAAAGAGATAAGTTTGTTGATTATAACGACGTTGGTGGTGTTGTTTTGTGTGAAAGTTTCCAAGGCATTGGATGATTGTGAATTGAATTATAATGATCCTCATAAAGTTGCATACACTATTGTTGTTGATAAACTTGGGAAAGGTAACTTCAACACAATCCAAAGTGCCATTAATTCTGTCCCTTCCCAAAGTACCAAATGGATTAGAATTCAAGTTTCTCCTGGCATATATACGGAAAAAGTGACGATACCCTCCGAAAAATCATGCATATTTCTAGATGGAAGTGGGCTTCAAGTGACTGAAATTCATTGGAATGACCATGAAACAACAGCTGCTAGTCCCACCTTTACTGCTTCTGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGAATACTTATAATGCACGTGGAAGTGTGATGAGACGTGAAGATATAAAACCAGCACTTGCAGCTCTAATCCAAGGAGACAAAGTGATATTTCATAAATGTGGTTTCATTGGTTTGCAAGATACTTTGTGGGATGGTCCTGGCCGTCATCTATTCACCCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTTGGTCAGTCTATCTATAAGGAATGTGTGATAAACATACCAGTCAATGCATATGCTCCATTACTAAATGAAGGTTTCATAACAGCTCAAGGAAAGGAGAATCCAAATGAAAGCAGTGGATTTGTATTCCTTAGATGCATAGTGCAGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTAACCCTGCTGGTTGGAATTCTTGGCTACAGGCTGGGCATGAGAGTGATTTGACGTATTCAGAGACCCGTTGTACTGGGCCAGGAGCAGATACTTCAAGCCGTGTGCCTTGGGTTAATAGATTGGATGCATTTCACATAAGAAGCTTCACAGATATTTCTTATATTGATCCACAAGATATTAAACGGATAAGAACAGATACTACACTTGATCTTAGCCAAAAGGCCGAGAAAGGTATGCTTTTAGAGTCCAATGAAACTCATGTTTATACTCCGCCGCTTGCTTCCATTTTGTGA

Coding sequence (CDS)

ATGGAACATTATTATTCGAAAGAGATAAGTTTGTTGATTATAACGACGTTGGTGGTGTTGTTTTGTGTGAAAGTTTCCAAGGCATTGGATGATTGTGAATTGAATTATAATGATCCTCATAAAGTTGCATACACTATTGTTGTTGATAAACTTGGGAAAGGTAACTTCAACACAATCCAAAGTGCCATTAATTCTGTCCCTTCCCAAAGTACCAAATGGATTAGAATTCAAGTTTCTCCTGGCATATATACGGAAAAAGTGACGATACCCTCCGAAAAATCATGCATATTTCTAGATGGAAGTGGGCTTCAAGTGACTGAAATTCATTGGAATGACCATGAAACAACAGCTGCTAGTCCCACCTTTACTGCTTCTGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGAATACTTATAATGCACGTGGAAGTGTGATGAGACGTGAAGATATAAAACCAGCACTTGCAGCTCTAATCCAAGGAGACAAAGTGATATTTCATAAATGTGGTTTCATTGGTTTGCAAGATACTTTGTGGGATGGTCCTGGCCGTCATCTATTCACCCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTTGGTCAGTCTATCTATAAGGAATGTGTGATAAACATACCAGTCAATGCATATGCTCCATTACTAAATGAAGGTTTCATAACAGCTCAAGGAAAGGAGAATCCAAATGAAAGCAGTGGATTTGTATTCCTTAGATGCATAGTGCAGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTAACCCTGCTGGTTGGAATTCTTGGCTACAGGCTGGGCATGAGAGTGATTTGACGTATTCAGAGACCCGTTGTACTGGGCCAGGAGCAGATACTTCAAGCCGTGTGCCTTGGGTTAATAGATTGGATGCATTTCACATAAGAAGCTTCACAGATATTTCTTATATTGATCCACAAGATATTAAACGGATAAGAACAGATACTACACTTGATCTTAGCCAAAAGGCCGAGAAAGGTATGCTTTTAGAGTCCAATGAAACTCATGTTTATACTCCGCCGCTTGCTTCCATTTTGTGA

Protein sequence

MEHYYSKEISLLIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQDIKRIRTDTTLDLSQKAEKGMLLESNETHVYTPPLASIL*

Homology

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BLAST of CSPI03G25040 vs. ExPASy Swiss-Prot
Match: Q3E9D3 (Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 2.2e-77
Identity = 138/302 (45.70%), Postives = 192/302 (63.58%), Query Frame = 0


Query: 39  PHKV---AYTIVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSC 98
           PH +    + ++VD+ G GNF TIQ AI+SVP  +T W  I V  G+Y EK+TIP +K  
Sbjct: 19  PHLIEAKPFEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPF 78

Query: 99  IFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYN--ARGSVMRREDIK 158
           I + G+G + T + W+DH + A SPTF   A N VV+ ITF N+YN  + G + +   + 
Sbjct: 79  IVIVGAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRV- 138

Query: 159 PALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVI 218
           PA+AA I GDK  F+  GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CVI
Sbjct: 139 PAVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVI 198

Query: 219 NIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLC 278
            +      P +  G+ITAQG+ N N+++GFVF+ C+V G G  +LGRA+RP+S VIF+  
Sbjct: 199 QVLGGQLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNS 258

Query: 279 FLPSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISY 336
            L   ++P GW  W   G+E  LTY+E  C G G++TS R  WV +L A  ++   D+S+
Sbjct: 259 NLTDVVDPLGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSF 318

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BLAST of CSPI03G25040 vs. ExPASy Swiss-Prot
Match: Q4PSQ5 (Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2)

HSP 1 Score: 290.8 bits (743), Expect = 2.2e-77
Identity = 138/295 (46.78%), Postives = 188/295 (63.73%), Query Frame = 0


Query: 41  KVAYTIVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDG 100
           ++AYTI VD  G GNF T+QSAI+S+   +  WIR+    GIY EKVTIP EK  I+L G
Sbjct: 30  QIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQG 89

Query: 101 SGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALI 160
            G++ T I ++DH+ T  S TFTA A ++V+ GITF+NTYN   +   + +I PA+AA +
Sbjct: 90  KGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPN--NKREIVPAVAARM 149

Query: 161 QGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAY 220
            GD+ +     F+GLQDTL+DG GRH + +C I G ID I G+GQS++KEC +N+ +  Y
Sbjct: 150 LGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNMTLGIY 209

Query: 221 APLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCIN 280
           AP    G ITA  + +P++  GFVF  C V G G   LGRA+   + VIF    L   + 
Sbjct: 210 APDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRLSDVVL 269

Query: 281 PAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYID 336
           P GW++W   G+E DLT+ E  CTG GADTS RVPW+ +L    +  F  +S+ID
Sbjct: 270 PIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFID 322

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BLAST of CSPI03G25040 vs. ExPASy Swiss-Prot
Match: Q4PSN0 (Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.3e-74
Identity = 136/292 (46.58%), Postives = 187/292 (64.04%), Query Frame = 0


Query: 46  IVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQV 105
           + VD+ G GNF TIQ AI+SVP  +  W  I V  G+Y EK+ IP EK  I L G+G ++
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 106 TEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYN--ARGSVMRREDIKPALAALIQGD 165
           T + W+DH + A SPTF+  A N VV+ ITF N+YN  ++G  M +    PA+AALI GD
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGK-MNKNPRTPAVAALIGGD 153

Query: 166 KVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPL 225
           K  F+  GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CVI +      P 
Sbjct: 154 KSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEPG 213

Query: 226 LNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAG 285
           L  G+ITAQG+ NP +++GF+F+ C+V G+G  FLGR +R +S VIF+   L   + P G
Sbjct: 214 L-AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEG 273

Query: 286 WNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYID 336
           W++W   GHE+ L ++E  C G GA+   RV WV +L    I++  D+S+I+
Sbjct: 274 WDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFIN 323

[Close]

BLAST of CSPI03G25040 vs. ExPASy Swiss-Prot
Match: O64479 (Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 4.0e-71
Identity = 130/297 (43.77%), Postives = 181/297 (60.94%), Query Frame = 0


Query: 41  KVAYTIVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDG 100
           ++A TI+V+      F T+QSAI+S+P Q+  WIRI +S GIY+EKVTIP  K  I++ G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 101 SGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALI 160
            G++ T I + DH+ T  S TFT+   N+++ GITF+N YN   S       KPA+AA++
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS---SSPTKPAVAAMM 155

Query: 161 QGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAY 220
            GDK       F G QDTL+D  GRH + +C I G ID I G  QSI++ C + + V  Y
Sbjct: 156 LGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIY 215

Query: 221 APLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCIN 280
            P    G ITAQG+++P +  GFVF  C V GSG   LGRA++ +S VIF+       I 
Sbjct: 216 PPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNIL 275

Query: 281 PAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ 338
           P GW++W   G E  +T+ E  CTG GADTS RVPW+ +     +  FT++++ID +
Sbjct: 276 PIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEE 329

[Close]

BLAST of CSPI03G25040 vs. ExPASy Swiss-Prot
Match: O04953 (Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2)

HSP 1 Score: 238.4 bits (607), Expect = 1.3e-61
Identity = 115/243 (47.33%), Postives = 153/243 (62.96%), Query Frame = 0


Query: 100 GSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRRE----DIKPA 159
           G G +VT I +N H  T  S TFT+   ++VV+ ++  NTYN   S+ +      DIKPA
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRLTSLTKANGMSWDIKPA 101

Query: 160 LAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINI 219
           +A  + GDK  F+ C F+GLQDT+WD  GRH F  CYIEG ID I G GQS+Y++C IN 
Sbjct: 102 VAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINA 161

Query: 220 PVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFL 279
              A A  ++ G+ITAQG+ + ++ SGFVFLR  V GS +V+LGRAY PFS VIF    L
Sbjct: 162 TAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDL 221

Query: 280 PSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYID 339
            S ++P GW SW   G+E   TY+E  C G G+D S RVPW+++L +F+ +    IS   
Sbjct: 222 SSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFI 281

[Close]

BLAST of CSPI03G25040 vs. ExPASy TrEMBL
Match: A0A0A0LB12 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G536660 PE=4 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 2.9e-197
Identity = 333/337 (98.81%), Postives = 334/337 (99.11%), Query Frame = 0


Query: 1   MEHYYSKEISLLIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFNTIQ 60
           MEHYYSKEISL IITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNF TIQ
Sbjct: 1   MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQ 60

Query: 61  SAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120
           SAINSVPSQSTKWIRIQ+SPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP
Sbjct: 61  SAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120

Query: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180
           TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW
Sbjct: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180

Query: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240
           DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES
Sbjct: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240

Query: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300
           SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE
Sbjct: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300

Query: 301 TRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ 338
           TRC GPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ
Sbjct: 301 TRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ 337

[Close]

BLAST of CSPI03G25040 vs. ExPASy TrEMBL
Match: A0A1S4DWQ1 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490078 PE=4 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.4e-156
Identity = 278/354 (78.53%), Postives = 298/354 (84.18%), Query Frame = 0


Query: 1   MEHYYSKEISLLIITTLVVLFC-VKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFNTI 60
           MEH YSKEI+LL ITTL++LFC V VSKALD CELNYNDPHKVAYTIVVDK G+GNF+TI
Sbjct: 1   MEH-YSKEITLL-ITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTI 60

Query: 61  QSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAAS 120
           QSAI+SVPSQS +WIRIQ+SPGIYT              DGSG +VTEI WNDH TTA S
Sbjct: 61  QSAIDSVPSQSNRWIRIQISPGIYT-------------YDGSGFRVTEIQWNDHATTATS 120

Query: 121 PTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTL 180
           PTFT+ AQNLVVQGITFRNTYNA GSV RREDIKPA+AALIQGDK IFHKCGFIGLQDTL
Sbjct: 121 PTFTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTL 180

Query: 181 WDGPGRHLFTQCYIEGVIDVISGFGQSI----------------YKECVINIPVNAYAPL 240
           WDGPGRHLFTQCYIEGVIDVISGFG  I                 +ECVINIP+NAYAPL
Sbjct: 181 WDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPL 240

Query: 241 LNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAG 300
           LNEGFITAQGKENP+ESSGFVF+RCIV+GSG VFLGRAYRPFSTVIFHLCFLPSCI+PAG
Sbjct: 241 LNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAG 300

Query: 301 WNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ 338
           WNSW Q GHES+LTYSETRC GPGADTSSRVPW+ RLD + IR FTDISYIDPQ
Sbjct: 301 WNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQ 339

[Close]

BLAST of CSPI03G25040 vs. ExPASy TrEMBL
Match: A0A6J1DWQ8 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111023848 PE=4 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 6.3e-128
Identity = 222/337 (65.88%), Postives = 266/337 (78.93%), Query Frame = 0


Query: 1   MEHYYSKEISLLIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFNTIQ 60
           MEHY   + ++ +I TL+      VS AL  CELNY +P++VAY+IVV+K G+GNF TIQ
Sbjct: 1   MEHY--SKGTIWLIPTLLFACLYNVSNAL-ACELNYQNPYRVAYSIVVNKFGRGNFRTIQ 60

Query: 61  SAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120
           SAI+S+P  STKWIR+Q+S G Y EKVTIP  KSCIF+DG+G +VTEI WNDH+TTA S 
Sbjct: 61  SAIDSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSA 120

Query: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180
           TFT+ A+NLVV+GI FRNTYNA GSV R+EDI PA+AALI GDK  F++C FIGLQDTLW
Sbjct: 121 TFTSFAENLVVRGIAFRNTYNAPGSVRRQEDIAPAVAALIMGDKASFYECRFIGLQDTLW 180

Query: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240
           DGPGRH F  CYIEGVIDVISG GQSIY++C+IN+P++ YAP+L+ G+ITAQGKENPNE 
Sbjct: 181 DGPGRHHFVNCYIEGVIDVISGSGQSIYEKCMINVPIHQYAPILSYGYITAQGKENPNEG 240

Query: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300
           SGFVF  C V G+G V+LGRAYRPFSTVIFH  FL   I+PAGWN W Q GHE+ LTY+E
Sbjct: 241 SGFVFNACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFIDPAGWNPWGQVGHETSLTYAE 300

Query: 301 TRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ 338
             C GPGA+ S RVPW+ RL    IR FTDIS+ID +
Sbjct: 301 LNCRGPGANISRRVPWLKRLHRAEIRHFTDISFIDQE 334

[Close]

BLAST of CSPI03G25040 vs. ExPASy TrEMBL
Match: A0A5D3DA76 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00550 PE=4 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 2.3e-125
Identity = 213/249 (85.54%), Postives = 225/249 (90.36%), Query Frame = 0


Query: 89  IPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMR 148
           IP +KSCIFLDGSG +VTEI WNDH TTA SPTFT+ AQNLV       NTYNA GSV R
Sbjct: 2   IPPDKSCIFLDGSGFRVTEIQWNDHATTATSPTFTSYAQNLV-------NTYNALGSVTR 61

Query: 149 REDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIY 208
           REDIKPA+AALIQGDK IFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIY
Sbjct: 62  REDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIY 121

Query: 209 KECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTV 268
           KECVINIP+NAYAPLLNEGFITAQGKENP+ESSGFVF+RCIV+GSG VFLGRAYRPFSTV
Sbjct: 122 KECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTV 181

Query: 269 IFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSF 328
           IFHLCFLPSCI+PAGWNSW Q GHES+LTYSETRC GPGADTSSRVPW+ RLD + IR F
Sbjct: 182 IFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRF 241

Query: 329 TDISYIDPQ 338
           TDISYIDPQ
Sbjct: 242 TDISYIDPQ 243

[Close]

BLAST of CSPI03G25040 vs. ExPASy TrEMBL
Match: A0A6J1A2L9 (Pectinesterase OS=Herrania umbratica OX=108875 GN=LOC110414390 PE=4 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 2.1e-91
Identity = 163/320 (50.94%), Postives = 212/320 (66.25%), Query Frame = 0


Query: 18  VVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQ 77
           +VL    +SKAL +C+LN  +   VA+TI VD+ GKG+F TIQ+AI+S+PS + KWIRI 
Sbjct: 29  IVLLYFSISKAL-ECQLNNANKFNVAHTIRVDQSGKGDFKTIQNAIDSIPSNNDKWIRIL 88

Query: 78  VSPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFR 137
           +SPG + EKVTIP+ K CIFL G+G ++T I W DH  T  S TFT+S  N+V +GITF+
Sbjct: 89  ISPGNFREKVTIPANKPCIFLRGAGTKLTSIEWGDHMDTCDSATFTSSPDNIVAKGITFK 148

Query: 138 NTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVI 197
           N YN +     ++ ++PALA  I GDK  F+ C F GLQDTLWD  GRH F  CYIEG +
Sbjct: 149 NIYNIQPGHDAKKKMEPALAVRIYGDKSAFYNCAFFGLQDTLWDVQGRHYFYNCYIEGAV 208

Query: 198 DVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVF 257
           D I G GQSIY+ C IN+    YAP    G+ITAQG+ +  + SGFVF  C+  GSG  +
Sbjct: 209 DFIFGGGQSIYERCEINLTAGKYAPAYPNGYITAQGRNSSADPSGFVFKYCVFTGSGKTY 268

Query: 258 LGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWV 317
           LGRAY  +S VI +   L   I P+GW++W    HE +L Y E  C GPGA+TS RVPW+
Sbjct: 269 LGRAYGAYSRVIIYNSVLSDAILPSGWDAWHYVHHEENLIYVEADCKGPGANTSKRVPWL 328

Query: 318 NRLDAFHIRSFTDISYIDPQ 338
            +L A  +  F +ISYID +
Sbjct: 329 RKLSASQLNQFLNISYIDKE 347

[Close]

BLAST of CSPI03G25040 vs. NCBI nr
Match: KAE8650757.1 (hypothetical protein Csa_017626 [Cucumis sativus])

HSP 1 Score: 770.0 bits (1987), Expect = 9.4e-219
Identity = 371/376 (98.67%), Postives = 372/376 (98.94%), Query Frame = 0


Query: 1   MEHYYSKEISLLIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFNTIQ 60
           MEHYYSKEISL IITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNF TIQ
Sbjct: 1   MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQ 60

Query: 61  SAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120
           SAINSVPSQSTKWIRIQ+SPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP
Sbjct: 61  SAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120

Query: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180
           TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW
Sbjct: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180

Query: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240
           DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES
Sbjct: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240

Query: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300
           SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE
Sbjct: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300

Query: 301 TRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQDIKRIRTDTTLDLSQKAEKGMLL 360
           TRC GPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQDIK IRTDTTLDLSQKAEKGMLL
Sbjct: 301 TRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQDIKLIRTDTTLDLSQKAEKGMLL 360

Query: 361 ESNETHVYTPPLASIL 377
           ESNETHVYTPPLASIL
Sbjct: 361 ESNETHVYTPPLASIL 376

[Close]

BLAST of CSPI03G25040 vs. NCBI nr
Match: XP_016900403.1 (PREDICTED: probable pectinesterase 55 [Cucumis melo])

HSP 1 Score: 562.4 bits (1448), Expect = 3.0e-156
Identity = 278/354 (78.53%), Postives = 298/354 (84.18%), Query Frame = 0


Query: 1   MEHYYSKEISLLIITTLVVLFC-VKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFNTI 60
           MEH YSKEI+LL ITTL++LFC V VSKALD CELNYNDPHKVAYTIVVDK G+GNF+TI
Sbjct: 1   MEH-YSKEITLL-ITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTI 60

Query: 61  QSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAAS 120
           QSAI+SVPSQS +WIRIQ+SPGIYT              DGSG +VTEI WNDH TTA S
Sbjct: 61  QSAIDSVPSQSNRWIRIQISPGIYT-------------YDGSGFRVTEIQWNDHATTATS 120

Query: 121 PTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTL 180
           PTFT+ AQNLVVQGITFRNTYNA GSV RREDIKPA+AALIQGDK IFHKCGFIGLQDTL
Sbjct: 121 PTFTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTL 180

Query: 181 WDGPGRHLFTQCYIEGVIDVISGFGQSI----------------YKECVINIPVNAYAPL 240
           WDGPGRHLFTQCYIEGVIDVISGFG  I                 +ECVINIP+NAYAPL
Sbjct: 181 WDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPL 240

Query: 241 LNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAG 300
           LNEGFITAQGKENP+ESSGFVF+RCIV+GSG VFLGRAYRPFSTVIFHLCFLPSCI+PAG
Sbjct: 241 LNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAG 300

Query: 301 WNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ 338
           WNSW Q GHES+LTYSETRC GPGADTSSRVPW+ RLD + IR FTDISYIDPQ
Sbjct: 301 WNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQ 339

[Close]

BLAST of CSPI03G25040 vs. NCBI nr
Match: XP_011652807.1 (probable pectinesterase 55 [Cucumis sativus])

HSP 1 Score: 536.2 bits (1380), Expect = 2.3e-148
Identity = 252/253 (99.60%), Postives = 252/253 (99.60%), Query Frame = 0


Query: 85  EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG 144
           EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG
Sbjct: 33  EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG 92

Query: 145 SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG 204
           SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG
Sbjct: 93  SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG 152

Query: 205 QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP 264
           QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP
Sbjct: 153 QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP 212

Query: 265 FSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFH 324
           FSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRC GPGADTSSRVPWVNRLDAFH
Sbjct: 213 FSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFH 272

Query: 325 IRSFTDISYIDPQ 338
           IRSFTDISYIDPQ
Sbjct: 273 IRSFTDISYIDPQ 285

[Close]

BLAST of CSPI03G25040 vs. NCBI nr
Match: XP_023524602.1 (LOW QUALITY PROTEIN: probable pectinesterase 55 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 483.8 bits (1244), Expect = 1.4e-132
Identity = 224/327 (68.50%), Postives = 272/327 (83.18%), Query Frame = 0


Query: 11  LLIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFNTIQSAINSVPSQS 70
           +L+ITTL++L C+ VSKAL DCELN NDP++VAYTIVVDK G GNF TIQSAI+S+PSQ+
Sbjct: 4   ILLITTLLLLLCLNVSKAL-DCELNPNDPNRVAYTIVVDKSGSGNFRTIQSAIDSIPSQN 63

Query: 71  TKWIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLV 130
           ++WIRIQ+S GIY+EKV I  EKSCIFL+G+G   TEI WNDH TTA S TFT+ A+NLV
Sbjct: 64  SRWIRIQISSGIYSEKVHISEEKSCIFLEGAGRSSTEIRWNDHATTATSATFTSIAENLV 123

Query: 131 VQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQ 190
           V+GITF+NTYN  GSV R+EDI PA+AALI+GDK IFHKCGF+GLQDTLWDG GRH +T+
Sbjct: 124 VKGITFKNTYNVPGSVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTE 183

Query: 191 CYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIV 250
           CYIEGVIDVISG GQSIY+ C INIPV+ YAP++  G+ITAQGKE+ ++++GFVF+ C V
Sbjct: 184 CYIEGVIDVISGAGQSIYESCEINIPVDRYAPVIGHGYITAQGKEDSSQTNGFVFIGCSV 243

Query: 251 QGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCTGPGADT 310
            GSG V+LGRAYRPFSTVI++  FL +CINPAGW+ W Q GHE+ LTY ETRC GPGADT
Sbjct: 244 IGSGTVYLGRAYRPFSTVIYYSSFLSACINPAGWDPWEQVGHEASLTYLETRCIGPGADT 303

Query: 311 SSRVPWVNRLDAFHIRSFTDISYIDPQ 338
           S RVPW+ +L    IR FT IS++D +
Sbjct: 304 SKRVPWLKKLSIDEIRRFTSISFVDQE 329

[Close]

BLAST of CSPI03G25040 vs. NCBI nr
Match: KAG6606930.1 (putative pectinesterase 55, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 471.5 bits (1212), Expect = 6.9e-129
Identity = 220/325 (67.69%), Postives = 267/325 (82.15%), Query Frame = 0


Query: 13  IITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFNTIQSAINSVPSQSTK 72
           +ITTL++L C+ VSKAL DCELN NDP++VAYTIVVDK G GNF TIQSAI+S+PSQ+++
Sbjct: 6   LITTLLMLLCLNVSKAL-DCELNPNDPNRVAYTIVVDKSGSGNFRTIQSAIDSIPSQNSR 65

Query: 73  WIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQ 132
           WIRIQ+S GIY EKV I  EKSCIFLDG+G   TEI WNDH TTA S TFT+ A+NLVV+
Sbjct: 66  WIRIQISSGIYLEKVHISEEKSCIFLDGAGRSSTEIRWNDHATTATSATFTSIAENLVVK 125

Query: 133 GITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCY 192
           GITF+NTYNA GSV R+EDI PA+AALI+GDK IFHKCGF+GLQDTLWDG GRH +T+CY
Sbjct: 126 GITFKNTYNAPGSVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECY 185

Query: 193 IEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQG 252
           IEGVIDVISG GQSIY+ C IN+PV+ YAP++  G+ITAQGKE+ ++++GFVF+ C V G
Sbjct: 186 IEGVIDVISGAGQSIYENCEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIG 245

Query: 253 SGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTSS 312
           SG V+LGRAYRPFSTVI++  FL +CI+PAGW+ W Q GHE+ LT +ET C G GADTS 
Sbjct: 246 SGTVYLGRAYRPFSTVIYYSSFLSACIDPAGWDPWEQVGHEASLTCAETHCVGLGADTSK 305

Query: 313 RVPWVNRLDAFHIRSFTDISYIDPQ 338
           RV W+ +L    IR FT IS+ID +
Sbjct: 306 RVSWLKKLSIDEIRHFTSISFIDQE 329

[Close]

BLAST of CSPI03G25040 vs. TAIR 10
Match: AT2G47280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 290.8 bits (743), Expect = 1.6e-78
Identity = 138/295 (46.78%), Postives = 188/295 (63.73%), Query Frame = 0


Query: 41  KVAYTIVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDG 100
           ++AYTI VD  G GNF T+QSAI+S+   +  WIR+    GIY EKVTIP EK  I+L G
Sbjct: 30  QIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQG 89

Query: 101 SGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALI 160
            G++ T I ++DH+ T  S TFTA A ++V+ GITF+NTYN   +   + +I PA+AA +
Sbjct: 90  KGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPN--NKREIVPAVAARM 149

Query: 161 QGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAY 220
            GD+ +     F+GLQDTL+DG GRH + +C I G ID I G+GQS++KEC +N+ +  Y
Sbjct: 150 LGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNMTLGIY 209

Query: 221 APLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCIN 280
           AP    G ITA  + +P++  GFVF  C V G G   LGRA+   + VIF    L   + 
Sbjct: 210 APDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRLSDVVL 269

Query: 281 PAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYID 336
           P GW++W   G+E DLT+ E  CTG GADTS RVPW+ +L    +  F  +S+ID
Sbjct: 270 PIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFID 322

[Close]

BLAST of CSPI03G25040 vs. TAIR 10
Match: AT5G18990.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 290.8 bits (743), Expect = 1.6e-78
Identity = 138/302 (45.70%), Postives = 192/302 (63.58%), Query Frame = 0


Query: 39  PHKV---AYTIVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSC 98
           PH +    + ++VD+ G GNF TIQ AI+SVP  +T W  I V  G+Y EK+TIP +K  
Sbjct: 19  PHLIEAKPFEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPF 78

Query: 99  IFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYN--ARGSVMRREDIK 158
           I + G+G + T + W+DH + A SPTF   A N VV+ ITF N+YN  + G + +   + 
Sbjct: 79  IVIVGAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRV- 138

Query: 159 PALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVI 218
           PA+AA I GDK  F+  GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CVI
Sbjct: 139 PAVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVI 198

Query: 219 NIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLC 278
            +      P +  G+ITAQG+ N N+++GFVF+ C+V G G  +LGRA+RP+S VIF+  
Sbjct: 199 QVLGGQLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNS 258

Query: 279 FLPSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISY 336
            L   ++P GW  W   G+E  LTY+E  C G G++TS R  WV +L A  ++   D+S+
Sbjct: 259 NLTDVVDPLGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSF 318

[Close]

BLAST of CSPI03G25040 vs. TAIR 10
Match: AT3G24130.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 281.6 bits (719), Expect = 9.5e-76
Identity = 136/292 (46.58%), Postives = 187/292 (64.04%), Query Frame = 0


Query: 46  IVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQV 105
           + VD+ G GNF TIQ AI+SVP  +  W  I V  G+Y EK+ IP EK  I L G+G ++
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 106 TEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYN--ARGSVMRREDIKPALAALIQGD 165
           T + W+DH + A SPTF+  A N VV+ ITF N+YN  ++G  M +    PA+AALI GD
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGK-MNKNPRTPAVAALIGGD 153

Query: 166 KVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPL 225
           K  F+  GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CVI +      P 
Sbjct: 154 KSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEPG 213

Query: 226 LNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAG 285
           L  G+ITAQG+ NP +++GF+F+ C+V G+G  FLGR +R +S VIF+   L   + P G
Sbjct: 214 L-AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEG 273

Query: 286 WNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYID 336
           W++W   GHE+ L ++E  C G GA+   RV WV +L    I++  D+S+I+
Sbjct: 274 WDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFIN 323

[Close]

BLAST of CSPI03G25040 vs. TAIR 10
Match: AT2G19150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 270.0 bits (689), Expect = 2.9e-72
Identity = 130/297 (43.77%), Postives = 181/297 (60.94%), Query Frame = 0


Query: 41  KVAYTIVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDG 100
           ++A TI+V+      F T+QSAI+S+P Q+  WIRI +S GIY+EKVTIP  K  I++ G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 101 SGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALI 160
            G++ T I + DH+ T  S TFT+   N+++ GITF+N YN   S       KPA+AA++
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS---SSPTKPAVAAMM 155

Query: 161 QGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAY 220
            GDK       F G QDTL+D  GRH + +C I G ID I G  QSI++ C + + V  Y
Sbjct: 156 LGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIY 215

Query: 221 APLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCIN 280
            P    G ITAQG+++P +  GFVF  C V GSG   LGRA++ +S VIF+       I 
Sbjct: 216 PPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNIL 275

Query: 281 PAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ 338
           P GW++W   G E  +T+ E  CTG GADTS RVPW+ +     +  FT++++ID +
Sbjct: 276 PIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEE 329

[Close]

BLAST of CSPI03G25040 vs. TAIR 10
Match: AT5G26810.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 238.4 bits (607), Expect = 9.2e-63
Identity = 115/243 (47.33%), Postives = 153/243 (62.96%), Query Frame = 0


Query: 100 GSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRRE----DIKPA 159
           G G +VT I +N H  T  S TFT+   ++VV+ ++  NTYN   S+ +      DIKPA
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRLTSLTKANGMSWDIKPA 101

Query: 160 LAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINI 219
           +A  + GDK  F+ C F+GLQDT+WD  GRH F  CYIEG ID I G GQS+Y++C IN 
Sbjct: 102 VAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINA 161

Query: 220 PVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFL 279
              A A  ++ G+ITAQG+ + ++ SGFVFLR  V GS +V+LGRAY PFS VIF    L
Sbjct: 162 TAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDL 221

Query: 280 PSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYID 339
            S ++P GW SW   G+E   TY+E  C G G+D S RVPW+++L +F+ +    IS   
Sbjct: 222 SSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFI 281

The following BLAST results are available for this feature:

BLAST of CSPI03G25040 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3E9D3	2.2e-77	45.70	Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1	[more]
Q4PSQ5	2.2e-77	46.78	Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2	[more]
Q4PSN0	1.3e-74	46.58	Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1	[more]
O64479	4.0e-71	43.77	Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1	[more]
O04953	1.3e-61	47.33	Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2	[more]

BLAST of CSPI03G25040 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LB12	2.9e-197	98.81	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G536660 PE=4 SV=1	[more]
A0A1S4DWQ1	1.4e-156	78.53	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490078 PE=4 SV=1	[more]
A0A6J1DWQ8	6.3e-128	65.88	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111023848 PE=4 SV=1	[more]
A0A5D3DA76	2.3e-125	85.54	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00550...	[more]
A0A6J1A2L9	2.1e-91	50.94	Pectinesterase OS=Herrania umbratica OX=108875 GN=LOC110414390 PE=4 SV=1	[more]

BLAST of CSPI03G25040 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8650757.1	9.4e-219	98.67	hypothetical protein Csa_017626 [Cucumis sativus]	[more]
XP_016900403.1	3.0e-156	78.53	PREDICTED: probable pectinesterase 55 [Cucumis melo]	[more]
XP_011652807.1	2.3e-148	99.60	probable pectinesterase 55 [Cucumis sativus]	[more]
XP_023524602.1	1.4e-132	68.50	LOW QUALITY PROTEIN: probable pectinesterase 55 [Cucurbita pepo subsp. pepo]	[more]
KAG6606930.1	6.9e-129	67.69	putative pectinesterase 55, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CSPI03G25040 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G47280.1	1.6e-78	46.78	Pectin lyase-like superfamily protein	[more]
AT5G18990.1	1.6e-78	45.70	Pectin lyase-like superfamily protein	[more]
AT3G24130.1	9.5e-76	46.58	Pectin lyase-like superfamily protein	[more]
AT2G19150.1	2.9e-72	43.77	Pectin lyase-like superfamily protein	[more]
AT5G26810.1	9.2e-63	47.33	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 46..335 e-value: 1.1E-54 score: 185.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 39..347 e-value: 4.1E-87 score: 294.5
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 11..337
None	No IPR available	PANTHER	PTHR31321:SF82	PECTINESTERASE	coord: 11..337
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 42..336

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G25040.1	CSPI03G25040.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity