CSPI03G24300 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G24300
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCCHC-type domain-containing protein
LocationChr3: 21373905 .. 21374465 (-)
RNA-Seq ExpressionCSPI03G24300
SyntenyCSPI03G24300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAGCGTCTCGCAGCAAATAAAGGAGAATGGTGGAATGCCGATTCTCTACCCGATGTTAACTCCACACAACTACACGGTGTGGACGATAAAGGCAGAAGAGGAGCCAGCGAGAGGAGCCGAAGTGGATGTGAAGAAAGACAAGAAGGCGGGTGCATACATTCTCCAATGCATCCTGGAAGACGTGCTTCTACAAATCACAAAGAAGAAGACCGCAAAGGAAATATGGGATAGTTTCAAGACAAGGTACTTGGGCAGTGAGCAGGTGCAGATGGTGAGAGTTCAAACCTTGAAGAGTGAGTTTAATGTTTTTCGGATGAAGGAAACTGAGACGATTGATGAGTTCGCAAGAAAAATCAGCGGATTAGCAAGCAAGTTCACCACCCTCGGAGTTGCACTTGAGGATTCATCATTGGTCAAAAAGCTCCTCGATTCCGTCCCCGACAAATATCTTTCCATTGTCACCGAAATCGAGCAATTCCAAGATCTCGAAACCATGTCGTTTGAAGAAGCAATTGGACGAATGATGGCGTACGAGGAACGAACAACACGACTCTGA

mRNA sequence

ATGGCAAGCGTCTCGCAGCAAATAAAGGAGAATGGTGGAATGCCGATTCTCTACCCGATGTTAACTCCACACAACTACACGGTGTGGACGATAAAGGCAGAAGAGGAGCCAGCGAGAGGAGCCGAAGTGGATGTGAAGAAAGACAAGAAGGCGGGTGCATACATTCTCCAATGCATCCTGGAAGACGTGCTTCTACAAATCACAAAGAAGAAGACCGCAAAGGAAATATGGGATAGTTTCAAGACAAGGTACTTGGGCAGTGAGCAGGTGCAGATGGTGAGAGTTCAAACCTTGAAGAGTGAGTTTAATGTTTTTCGGATGAAGGAAACTGAGACGATTGATGAGTTCGCAAGAAAAATCAGCGGATTAGCAAGCAAGTTCACCACCCTCGGAGTTGCACTTGAGGATTCATCATTGGTCAAAAAGCTCCTCGATTCCGTCCCCGACAAATATCTTTCCATTGTCACCGAAATCGAGCAATTCCAAGATCTCGAAACCATGTCGTTTGAAGAAGCAATTGGACGAATGATGGCGTACGAGGAACGAACAACACGACTCTGA

Coding sequence (CDS)

ATGGCAAGCGTCTCGCAGCAAATAAAGGAGAATGGTGGAATGCCGATTCTCTACCCGATGTTAACTCCACACAACTACACGGTGTGGACGATAAAGGCAGAAGAGGAGCCAGCGAGAGGAGCCGAAGTGGATGTGAAGAAAGACAAGAAGGCGGGTGCATACATTCTCCAATGCATCCTGGAAGACGTGCTTCTACAAATCACAAAGAAGAAGACCGCAAAGGAAATATGGGATAGTTTCAAGACAAGGTACTTGGGCAGTGAGCAGGTGCAGATGGTGAGAGTTCAAACCTTGAAGAGTGAGTTTAATGTTTTTCGGATGAAGGAAACTGAGACGATTGATGAGTTCGCAAGAAAAATCAGCGGATTAGCAAGCAAGTTCACCACCCTCGGAGTTGCACTTGAGGATTCATCATTGGTCAAAAAGCTCCTCGATTCCGTCCCCGACAAATATCTTTCCATTGTCACCGAAATCGAGCAATTCCAAGATCTCGAAACCATGTCGTTTGAAGAAGCAATTGGACGAATGATGGCGTACGAGGAACGAACAACACGACTCTGA

Protein sequence

MASVSQQIKENGGMPILYPMLTPHNYTVWTIKAEEEPARGAEVDVKKDKKAGAYILQCILEDVLLQITKKKTAKEIWDSFKTRYLGSEQVQMVRVQTLKSEFNVFRMKETETIDEFARKISGLASKFTTLGVALEDSSLVKKLLDSVPDKYLSIVTEIEQFQDLETMSFEEAIGRMMAYEERTTRL*
Homology
BLAST of CSPI03G24300 vs. ExPASy TrEMBL
Match: Q0JH75 (Os01g0879800 protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0879800 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 1.2e-47
Identity = 105/191 (54.97%), Postives = 135/191 (70.68%), Query Frame = 0

Query: 3   SVSQQIKENGGMPILYPMLTPHNYTVWTIKAEE-----------EPARGAEVDVKKDKKA 62
           S S+ ++E G + + YP+LT  NY+ W IK E            EPA    VD+KKDK+A
Sbjct: 16  SASRSMRE-GIVSLPYPVLTSTNYSTWAIKMEANMEAQGIWDAIEPAADEAVDIKKDKQA 75

Query: 63  GAYILQCILEDVLLQITKKKTAKEIWDSFKTRYLGSEQVQMVRVQTLKSEFNVFRMKETE 122
            A +   + EDVL QI KKKTAKE+W+S KTR+LG E+V+  RVQTLKS+F    MK+TE
Sbjct: 76  RACLFGAVPEDVLQQIAKKKTAKEVWESLKTRFLGVERVKKARVQTLKSDFKALHMKDTE 135

Query: 123 TIDEFARKISGLASKFTTLGVALEDSSLVKKLLDSVPDKYLSIVTEIEQFQDLETMSFEE 182
           +IDEFA KISGLA+K + LGV ++D   VKKLLDSVPDK+L ++  IEQF DL++M F+E
Sbjct: 136 SIDEFAGKISGLANKLSDLGVTMDDDEQVKKLLDSVPDKFLHVIAAIEQFSDLDSMPFDE 195

BLAST of CSPI03G24300 vs. ExPASy TrEMBL
Match: Q2QUP7 (Laccase OS=Oryza sativa subsp. japonica OX=39947 GN=LOC_Os12g15530 PE=3 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 1.2e-47
Identity = 105/191 (54.97%), Postives = 135/191 (70.68%), Query Frame = 0

Query: 3   SVSQQIKENGGMPILYPMLTPHNYTVWTIKAEE-----------EPARGAEVDVKKDKKA 62
           S S+ ++E G + + YP+LT  NY+ W IK E            EPA    VD+KKDK+A
Sbjct: 429 SASRSMRE-GIVSLPYPVLTSTNYSTWAIKMEANMEAQGIWDAIEPAADEAVDIKKDKQA 488

Query: 63  GAYILQCILEDVLLQITKKKTAKEIWDSFKTRYLGSEQVQMVRVQTLKSEFNVFRMKETE 122
            A +   + EDVL QI KKKTAKE+W+S KTR+LG E+V+  RVQTLKS+F    MK+TE
Sbjct: 489 RACLFGAVPEDVLQQIAKKKTAKEVWESLKTRFLGVERVKKARVQTLKSDFKALHMKDTE 548

Query: 123 TIDEFARKISGLASKFTTLGVALEDSSLVKKLLDSVPDKYLSIVTEIEQFQDLETMSFEE 182
           +IDEFA KISGLA+K + LGV ++D   VKKLLDSVPDK+L ++  IEQF DL++M F+E
Sbjct: 549 SIDEFAGKISGLANKLSDLGVTMDDDEQVKKLLDSVPDKFLHVIAAIEQFSDLDSMPFDE 608

BLAST of CSPI03G24300 vs. ExPASy TrEMBL
Match: A0A5N6NYJ8 (CCHC-type domain-containing protein OS=Mikania micrantha OX=192012 GN=E3N88_16352 PE=4 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 2.7e-47
Identity = 99/193 (51.30%), Postives = 134/193 (69.43%), Query Frame = 0

Query: 1   MASVSQQIKENGGMPILYPMLTPHNYTVWTIKAEE-----------EPARGAEVDVKKDK 60
           +A+ S   K+   + +  P LT  NYT W IK E            EP  GA VD KK+K
Sbjct: 412 VATTSATPKDPSSVSLQIPSLTATNYTTWAIKMEAVMDAQGLWESIEPTPGAAVDEKKNK 471

Query: 61  KAGAYILQCILEDVLLQITKKKTAKEIWDSFKTRYLGSEQVQMVRVQTLKSEFNVFRMKE 120
            + A+I Q I EDVLLQ+ KKKTAKEIWDS K RY+G+++VQ  R+ TLK+EF + +MKE
Sbjct: 472 TSRAFIFQAIPEDVLLQVAKKKTAKEIWDSLKIRYMGADRVQKARLHTLKNEFELLKMKE 531

Query: 121 TETIDEFARKISGLASKFTTLGVALEDSSLVKKLLDSVPDKYLSIVTEIEQFQDLETMSF 180
            E++DEFA K++G+ +K+ +LG  ++D  LV+KLLDSVPDKYL +V  IEQ+ D++TM F
Sbjct: 532 GESVDEFAGKLAGMVTKYNSLGYTIKDEDLVRKLLDSVPDKYLQLVASIEQYSDIDTMPF 591

Query: 181 EEAIGRMMAYEER 183
           +EAIGR+ AYE+R
Sbjct: 592 DEAIGRLKAYEDR 604

BLAST of CSPI03G24300 vs. ExPASy TrEMBL
Match: A0A5N6MA89 (Uncharacterized protein OS=Mikania micrantha OX=192012 GN=E3N88_33044 PE=3 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 1.4e-46
Identity = 101/185 (54.59%), Postives = 130/185 (70.27%), Query Frame = 0

Query: 9   KENGGMPILYPMLTPHNYTVWTIKAEE-----------EPARGAEVDVKKDKKAGAYILQ 68
           KE G + +  P LT  NYT W+IK E            E  +G  VD KK K A A++ Q
Sbjct: 596 KEAGSVSLQIPNLTSTNYTTWSIKMEAIMDAQGLWESIETPQGLAVDEKKSKTARAFLFQ 655

Query: 69  CILEDVLLQITKKKTAKEIWDSFKTRYLGSEQVQMVRVQTLKSEFNVFRMKETETIDEFA 128
            I EDVLLQ+ KKKTAKE+W+S K RYLG+++VQ  R+ TLKSEF+  RM+ETE+IDE+A
Sbjct: 656 AIPEDVLLQVAKKKTAKEVWESLKLRYLGADRVQKARLNTLKSEFDALRMRETESIDEYA 715

Query: 129 RKISGLASKFTTLGVALEDSSLVKKLLDSVPDKYLSIVTEIEQFQDLETMSFEEAIGRMM 183
            K+S + SK ++LG  LEDS +V+KLLD+VPDKYL +V  IEQ  DL+TM FEEA+GR+ 
Sbjct: 716 GKLSAMISKHSSLGQTLEDSMVVRKLLDTVPDKYLQLVASIEQTADLDTMPFEEAVGRLK 775

BLAST of CSPI03G24300 vs. ExPASy TrEMBL
Match: A0A5N6M0G6 (Uncharacterized protein OS=Mikania micrantha OX=192012 GN=E3N88_34946 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 3.0e-46
Identity = 100/193 (51.81%), Postives = 133/193 (68.91%), Query Frame = 0

Query: 1   MASVSQQIKENGGMPILYPMLTPHNYTVWTIKAEE-----------EPARGAEVDVKKDK 60
           +A+ SQ  KE G + +  P LT  NYT W IK E            EP  GA VD +K+K
Sbjct: 3   VATTSQAPKEAGSVSLQIPTLTATNYTTWAIKMEAVMDAQGLWECVEPVAGATVDERKNK 62

Query: 61  KAGAYILQCILEDVLLQITKKKTAKEIWDSFKTRYLGSEQVQMVRVQTLKSEFNVFRMKE 120
            A A+I Q I EDVL+Q+ KKKTA EIW+S K RY+G+++VQ  R+ TLK+EF + +MKE
Sbjct: 63  TARAFIFQAIPEDVLMQVAKKKTAMEIWESLKARYVGADRVQKARLNTLKTEFELLKMKE 122

Query: 121 TETIDEFARKISGLASKFTTLGVALEDSSLVKKLLDSVPDKYLSIVTEIEQFQDLETMSF 180
            E+IDEFA K+S + +K+ +LG  LED  LV KLLDSVP+K+L +V  IEQ+ D+++M F
Sbjct: 123 GESIDEFAGKLSSMVAKYNSLGSTLEDKVLVGKLLDSVPEKFLQLVASIEQYSDVDSMPF 182

Query: 181 EEAIGRMMAYEER 183
           EEAIGR+ AYE+R
Sbjct: 183 EEAIGRLKAYEDR 195

BLAST of CSPI03G24300 vs. NCBI nr
Match: XP_031745073.1 (uncharacterized protein LOC116405251 [Cucumis sativus])

HSP 1 Score: 309.3 bits (791), Expect = 2.3e-80
Identity = 165/197 (83.76%), Postives = 168/197 (85.28%), Query Frame = 0

Query: 1   MASVSQQIKENGGMPILYPMLTPHNYTVWTIKAEE-----------EPARGAEVDVKKDK 60
           M S SQQ KENGGMPILYPMLTPHNYTVWTIKAE            EPARGAEVDVKKDK
Sbjct: 1   MTSTSQQTKENGGMPILYPMLTPHNYTVWTIKAEAILDAQGVWEAIEPARGAEVDVKKDK 60

Query: 61  KAGAYILQCILEDVLLQITKKKTAKEIWDSFKTRYLGSEQVQMVRVQTLKSEFNVFRMKE 120
           KA AYILQCI EDVLLQI KKKTAKEIWDS KTRYLGSE+V+M RVQTLK EFNV RMKE
Sbjct: 61  KACAYILQCIPEDVLLQIAKKKTAKEIWDSLKTRYLGSERVKMARVQTLKREFNVLRMKE 120

Query: 121 TETIDEFARKISGLASKFTTLGVALEDSSLVKKLLDSVPDKYLSIVTEIEQFQDLETMSF 180
           TE IDEFA KISGLASKFTTLGVAL+DSSLVKKLLDSVPDKYL IV  IEQFQDLETM F
Sbjct: 121 TEMIDEFAGKISGLASKFTTLGVALKDSSLVKKLLDSVPDKYLPIVAGIEQFQDLETMPF 180

Query: 181 EEAIGRMMAYEERTTRL 187
           EEAIGRM AYEERTTRL
Sbjct: 181 EEAIGRMKAYEERTTRL 197

BLAST of CSPI03G24300 vs. NCBI nr
Match: XP_031737549.1 (uncharacterized protein LOC116402439 [Cucumis sativus])

HSP 1 Score: 308.9 bits (790), Expect = 3.0e-80
Identity = 165/197 (83.76%), Postives = 170/197 (86.29%), Query Frame = 0

Query: 1   MASVSQQIKENGGMPILYPMLTPHNYTVWTIKAEE-----------EPARGAEVDVKKDK 60
           MAS+SQQ KENGGM ILY MLTPHNYTVWTIKAE            EPARGAEVDVKKDK
Sbjct: 1   MASISQQTKENGGMSILYSMLTPHNYTVWTIKAEAILDAQGVREAIEPARGAEVDVKKDK 60

Query: 61  KAGAYILQCILEDVLLQITKKKTAKEIWDSFKTRYLGSEQVQMVRVQTLKSEFNVFRMKE 120
           KA +YILQCI EDVLLQI KKKTAKEIWDS KTRYLGSE+V+M RVQTLKSEF+V RMKE
Sbjct: 61  KARSYILQCIPEDVLLQIAKKKTAKEIWDSLKTRYLGSERVKMARVQTLKSEFSVLRMKE 120

Query: 121 TETIDEFARKISGLASKFTTLGVALEDSSLVKKLLDSVPDKYLSIVTEIEQFQDLETMSF 180
           TETIDEFA KISGLASKFTTLGVALEDSSLVKKLLDSVPDKYL IV  IEQFQDLETM F
Sbjct: 121 TETIDEFAGKISGLASKFTTLGVALEDSSLVKKLLDSVPDKYLPIVVGIEQFQDLETMPF 180

Query: 181 EEAIGRMMAYEERTTRL 187
           EEAIGRM AYEERTTRL
Sbjct: 181 EEAIGRMKAYEERTTRL 197

BLAST of CSPI03G24300 vs. NCBI nr
Match: XP_011658449.1 (uncharacterized protein LOC105436008 [Cucumis sativus])

HSP 1 Score: 295.0 bits (754), Expect = 4.4e-76
Identity = 157/197 (79.70%), Postives = 163/197 (82.74%), Query Frame = 0

Query: 1   MASVSQQIKENGGMPILYPMLTPHNYTVWTIKAEE-----------EPARGAEVDVKKDK 60
           MASVSQQ KENG MPI YPML PHNYT+WTIK E            EP RG EVDVKKDK
Sbjct: 1   MASVSQQTKENGRMPIFYPMLCPHNYTMWTIKTEAILDTQEVWEAIEPIRGLEVDVKKDK 60

Query: 61  KAGAYILQCILEDVLLQITKKKTAKEIWDSFKTRYLGSEQVQMVRVQTLKSEFNVFRMKE 120
           KA  YILQCILEDVLLQI KKK+AKEIWD+ KTRYLGSE+V+M RV  LKSEFNV RMKE
Sbjct: 61  KACVYILQCILEDVLLQIAKKKSAKEIWDNLKTRYLGSERVKMARVGILKSEFNVLRMKE 120

Query: 121 TETIDEFARKISGLASKFTTLGVALEDSSLVKKLLDSVPDKYLSIVTEIEQFQDLETMSF 180
           TETIDEFA KIS LASKFTTLGVALEDSSLVKKLLDSVPDKYL IVT IEQFQDLETM F
Sbjct: 121 TETIDEFAGKISKLASKFTTLGVALEDSSLVKKLLDSVPDKYLLIVTRIEQFQDLETMPF 180

Query: 181 EEAIGRMMAYEERTTRL 187
           EE IGRMM YEERTT+L
Sbjct: 181 EETIGRMMVYEERTTQL 197

BLAST of CSPI03G24300 vs. NCBI nr
Match: XP_031741713.1 (uncharacterized protein LOC116403908 [Cucumis sativus])

HSP 1 Score: 280.4 bits (716), Expect = 1.1e-71
Identity = 151/183 (82.51%), Postives = 154/183 (84.15%), Query Frame = 0

Query: 14  MPILYPMLTPHNYTVWTIKAEE-----------EPARGAEVDVKKDKKAGAYILQCILED 73
           M ILYPMLTPHNYTVWTIKAE            EPARGAEVDVKKDKKA AYILQCI ED
Sbjct: 1   MSILYPMLTPHNYTVWTIKAEAILNAQGVWEAIEPARGAEVDVKKDKKARAYILQCIPED 60

Query: 74  VLLQITKKKTAKEIWDSFKTRYLGSEQVQMVRVQTLKSEFNVFRMKETETIDEFARKISG 133
           VLLQI KKKTAKEIWDS KTRYLGSE+V+M RVQTLKSEFNV RMKE ETIDEF  KISG
Sbjct: 61  VLLQIAKKKTAKEIWDSLKTRYLGSERVKMARVQTLKSEFNVLRMKEIETIDEFVGKISG 120

Query: 134 LASKFTTLGVALEDSSLVKKLLDSVPDKYLSIVTEIEQFQDLETMSFEEAIGRMMAYEER 186
           LASKFTTL VALEDSSLV KLLDSVPDKYL IV  I+QFQDLETM FEEAIGRM AYEER
Sbjct: 121 LASKFTTLRVALEDSSLVNKLLDSVPDKYLPIVVGIKQFQDLETMPFEEAIGRMKAYEER 180

BLAST of CSPI03G24300 vs. NCBI nr
Match: XP_042450925.1 (uncharacterized protein LOC122035851 [Zingiber officinale])

HSP 1 Score: 260.8 bits (665), Expect = 9.2e-66
Identity = 134/189 (70.90%), Postives = 148/189 (78.31%), Query Frame = 0

Query: 9   KENGGMPILYPMLTPHNYTVWTIKAEE-----------EPARGAEVDVKKDKKAGAYILQ 68
           KENGG+   YPML PHNYTVW IK E            EPA GA+VD KKDKKA AYILQ
Sbjct: 10  KENGGVSFPYPMLNPHNYTVWAIKTEAILDAQGVWETVEPAEGAQVDAKKDKKARAYILQ 69

Query: 69  CILEDVLLQITKKKTAKEIWDSFKTRYLGSEQVQMVRVQTLKSEFNVFRMKETETIDEFA 128
           C+ ED+LLQI  KKTAKE+WDS KTRYLGS++V+  RVQTLKSEF+  RMKETETIDEFA
Sbjct: 70  CVPEDILLQIANKKTAKEVWDSLKTRYLGSDRVKKARVQTLKSEFDALRMKETETIDEFA 129

Query: 129 RKISGLASKFTTLGVALEDSSLVKKLLDSVPDKYLSIVTEIEQFQDLETMSFEEAIGRMM 187
            K+S L+SKF+TLG  LEDSSLVKKLLDSVPDK+  IV  IEQF DLETM FEEAIGR+ 
Sbjct: 130 GKLSALSSKFSTLGATLEDSSLVKKLLDSVPDKFFPIVAGIEQFHDLETMPFEEAIGRLK 189

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
Q0JH751.2e-4754.97Os01g0879800 protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0879800 PE... [more]
Q2QUP71.2e-4754.97Laccase OS=Oryza sativa subsp. japonica OX=39947 GN=LOC_Os12g15530 PE=3 SV=1[more]
A0A5N6NYJ82.7e-4751.30CCHC-type domain-containing protein OS=Mikania micrantha OX=192012 GN=E3N88_1635... [more]
A0A5N6MA891.4e-4654.59Uncharacterized protein OS=Mikania micrantha OX=192012 GN=E3N88_33044 PE=3 SV=1[more]
A0A5N6M0G63.0e-4651.81Uncharacterized protein OS=Mikania micrantha OX=192012 GN=E3N88_34946 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_031745073.12.3e-8083.76uncharacterized protein LOC116405251 [Cucumis sativus][more]
XP_031737549.13.0e-8083.76uncharacterized protein LOC116402439 [Cucumis sativus][more]
XP_011658449.14.4e-7679.70uncharacterized protein LOC105436008 [Cucumis sativus][more]
XP_031741713.11.1e-7182.51uncharacterized protein LOC116403908 [Cucumis sativus][more]
XP_042450925.19.2e-6670.90uncharacterized protein LOC122035851 [Zingiber officinale][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 46..183
e-value: 1.5E-24
score: 86.3
NoneNo IPR availablePANTHERPTHR34676:SF11ZINC FINGER, CCHC-TYPE-RELATEDcoord: 3..183
NoneNo IPR availablePANTHERPTHR34676FAMILY NOT NAMEDcoord: 3..183

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G24300.1CSPI03G24300.1mRNA