CSPI03G23680 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G23680
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionMediator of RNA polymerase II transcription subunit 6
LocationChr3: 20691359 .. 20695007 (+)
RNA-Seq ExpressionCSPI03G23680
SyntenyCSPI03G23680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACAATTGTTTGAAAAATAATGTTGTTATACAGTTTGATTGCTAAGACTGCTATCCATTGTGGTTGCAATACAAATTCATCTCCATACCGTGGAGGTTGATACGTTTTTGTTACATGACTTCTCCCTCTTCACTCTCGGTCGTGATCGTCGGGAAACAAAGAAAAAGGGTGTAGTGCTTTCACTTACGGTGGAGGGATCTGTAAAATTCAACACTTCGTGGCCAGAAAAGTAAAATAGCGCACAAATGGCAACTCCACCACCACCGACGGCTCCGCCAGGGACCGGGAATTTCGAGGGGGGGCCGCTGCCAGCAGCGCCTCAGCCTCCGGGCACGGACATGACTGGAATTTGCTTTAGAGACCAGCTTTGGCTCAATACTTACCCTCTTGATCGAAACTTGGTTTTCGATTACTTCGCCTTGTCGCCTTTCTACGACTGGACTTGTAACAACGAACAACTCAGAATGCGCTCCATTCACCCCCTCGATCTTTCTCAACTCTCGTACGATTTCTCGTCTTCTTTTTTTTTTTTTTTTTTTAAATTTCTATTGACAGGATTTCGTTTTCTCTATTAATTGATTTCTTCGTTTAATTGATTGTATGTATCTTACATATTTCCCCCCGTACTTGGATAATTCAACCTTGTTCTTTATATGTTCTTGTTTTATTAAGATTTCCGAAATTTGTGAATTGCAACCCTAGGATTCAGTTTCATTTAGGCATTACCATGATGGGTAATCTTCTGTTCAACAGAAAAATGACAGGCATAGAGTACATGCTGAATGAAGTTATGGAGCCTCACCTTTTCGTTTTCAGAAAGCAAAAAAGGGATGGTCCTGAGAAAGTCACACCGATGCTGACTTATTATATCTTGGATGGTTCAATCTACCAGGCGCCTCAACTTTGCAGTGTTTTTGCTGCTCGCGTTGTGAGATTGGCATACTTATCTTTCTTTGTCACTTTCATTTGTTTGATGCAATCTTAGTTTTTCTACTTTTTTCCTCATAATTCTTGCATTTGGATAATTTCTTAGTTCCATTTAGTAAATTATCGTGTTGGGTAACATCTCTTCAACTGGAAATTTAATTGCTGGATTCCTTTTTTCTTCTTTGTTTTGAAATTAATGTAGAATTTGTTTAAAACTGCAAGGCTATTATGTATTCTGCTTCTCTCTTCCTCTGTTTTTTTGTAATCTTACATTGAGTCTTCATCATACTGCAGAGTCGGGCACTGTATTACATATCAAAGGCCTTTACCACTGCTTCGTCAAAGTTGGAGAAGATAGGATATGGTATCTTCTAGCTATTAGTGATATATTTTTTGCTCACACTATTTTAAGTTATAGTTAAAAGATACTCACCAATGACTTTACACCTCACGGTTCTGCTATCTTAACACACATTTTTTAAGTTTTATTTAAGTCGTGCTAATTTTCTTTGAAAGAATTCTTTGAGCTTTTCAAGATTAACAGTTGGTTTCTTATTTTGAGATCACTTAACCTCGGTTTGGTAACCATTTTGTTTTTTGTTTTTAACTTTTTATAATTAAACCTATTTCCTCCTCATGTCTTACAATTTTGCATCTTTCTAAAATACAATGGTTGAACGCTTAGCCAAATTCCAAAAATAAGAACAACTTTTTAAAAGCTACTTTTTAATTTTCAAATTTTGACTTGGTTTTTAAAACCATCAGTATAATGTAGATAACAAAGGAAGAAAGTTGGAGGTGGAAATAGTGTTTTAGGCTTAAGTTTTCAAAAACAAAAACCAAAAACAAAATGGTTACTGTGAGGACTCTTCCTAAGAAGAACAAGATCTCAATTTATTGAATTATATCTATGAAGAAATAAAAGATAATCCAAGAATAAAGGAACTTGTGAGCCTCCCTCTTGAGTATTTTCACCCAAGCCCTAGATCACTCCACAAATTAACCTCCCTTCATTCACTCATTCTCTTGCTATTTATGCAACATGCTGTAGAAATTTTCATCGAAAGACCATTATACTAACATGCTAATATTCTTATCTAGGTATCTAACAGTCACCAAATGGGCATTGGCCTTAGTGTTTAGTTTTCTCTTGTTTTATTTGATTAGGATTGTTTCAGTTTTCTGCGTAGATCTTACACTTTGTTAAAGGGTTTGTGGGTTTGTTGTTTTCTTTTTGAACTTTTGTATCTTGAGCATCAACCTCTTTACATCATTTCAGAGAATTAGAGACTCCTCAATACAAAAAACCAAGAAAGACCAAATTTTGATGCTTTCAGGCTTTAGATAGTTGATATTTAAAGCACTCCAGTAGGCTTTATGAATGTTCTTTTTTATTCAGACTTCATTCTGAAATCAAATTTTTGGGTCTTATATGGAATAGTTCATTCACATATGAAATGTAGAATGAACTTTAAAGATACAACACTGCCTAAATGCGTAACAAACTGTTTTCTCATTTCTGGTTCAGTTGATTCTGAAAATGAGAGTGAAGAAGTAAAACCTGCAAAAGAAACCATTAACTTTAAGGAAGTAAAGCGAGTTGATCATATTCTTGCATCTTTACAGCGCAAGGTAACTTTTTTCAACATCACAAATCATCTACTCTCTATTGACATAATCTGCATGAGCTTCATCTTTACTATAACAGAAAGTTAATGTATAAATTGCGAAGTGCTAATTTATCTATTAAGAAGAAAGTGGTGAGAATTTGTATGCAGTTTTCCTATAAGTCATTATCACATTTCATTTAATAACAACAGAACTTTTTTGAAGTTTCATCAGTTTATTTAAGTTTTGAACAATATTACCAAACCTAATCTAACTACTCACGAACCACTTACTCATTTTGAATGGAGAACTAATGACTTAGGCTTCAAATGTGTCCAGATTCAAATATATTGTACCTGTAAATAATTGAAATTAATTCAATTGAACTTGTAAAACAATTAAATTATCTAAATCGATAAACTGCTGGTATAGTATACAAATGACTTTATGTGTGTCCATATTAATGGTTTATATTTTTCATATTGAAGATTTGGCTGGTCTTTTTGCCTATCCTAATTAAGAGACAAATCTTCTTGTTAAAAATTACAGCTGCCACCTGCCCCACCACCCCCACCATTTCCTGAAGGTTATGCTCCTGCTGCCACAGCAGAGACAGAAAAAGGCCCTGAAAATCAGCAAGGAGAGTCACAACAGCCTTCTGCAGATCCCATTATTGATCAGGGTCCAGCTAAAAGGATGAAATTTTCAAGTGTTTAGGCTACCTTGCCTGTTGAGAAGCCTATTTAATCCTACAATTGAAATTCAACATGGATGACATCCCTGCACAAAGCATGGTGAGTTGTGAACACACACACACACACACACACACACACACCCCCAAAAAAAAAAAGAAGAAGAAAAGAGGAAAAAGGTCAAAGAGTTGGAATCTGCCTTGTGAAAAGCCTGTAGTTATAGACTTAACTTGTGATAATCCATTGTTTTAGTAGTAACTTGTACTACACTACCATGTAACCATCACTTCCTGATTTTGTTAGCTAGAATTGTTTGATACTCATATGACATACCACTAGTTGCTGTGTCTTTGTATTGTGATAACTTGGGAAGAAGGTTTTTAATATTGAACATGGTAATATAATTCTGCAATATCTTGGGC

mRNA sequence

CACAATTGTTTGAAAAATAATGTTGTTATACAGTTTGATTGCTAAGACTGCTATCCATTGTGGTTGCAATACAAATTCATCTCCATACCGTGGAGGTTGATACGTTTTTGTTACATGACTTCTCCCTCTTCACTCTCGGTCGTGATCGTCGGGAAACAAAGAAAAAGGGTGTAGTGCTTTCACTTACGGTGGAGGGATCTGTAAAATTCAACACTTCGTGGCCAGAAAAGTAAAATAGCGCACAAATGGCAACTCCACCACCACCGACGGCTCCGCCAGGGACCGGGAATTTCGAGGGGGGGCCGCTGCCAGCAGCGCCTCAGCCTCCGGGCACGGACATGACTGGAATTTGCTTTAGAGACCAGCTTTGGCTCAATACTTACCCTCTTGATCGAAACTTGGTTTTCGATTACTTCGCCTTGTCGCCTTTCTACGACTGGACTTGTAACAACGAACAACTCAGAATGCGCTCCATTCACCCCCTCGATCTTTCTCAACTCTCAAAAATGACAGGCATAGAGTACATGCTGAATGAAGTTATGGAGCCTCACCTTTTCGTTTTCAGAAAGCAAAAAAGGGATGGTCCTGAGAAAGTCACACCGATGCTGACTTATTATATCTTGGATGGTTCAATCTACCAGGCGCCTCAACTTTGCAGTGTTTTTGCTGCTCGCGTTAGTCGGGCACTGTATTACATATCAAAGGCCTTTACCACTGCTTCGTCAAAGTTGGAGAAGATAGGATATGTTGATTCTGAAAATGAGAGTGAAGAAGTAAAACCTGCAAAAGAAACCATTAACTTTAAGGAAGTAAAGCGAGTTGATCATATTCTTGCATCTTTACAGCGCAAGCTGCCACCTGCCCCACCACCCCCACCATTTCCTGAAGGTTATGCTCCTGCTGCCACAGCAGAGACAGAAAAAGGCCCTGAAAATCAGCAAGGAGAGTCACAACAGCCTTCTGCAGATCCCATTATTGATCAGGGTCCAGCTAAAAGGATGAAATTTTCAAGTGTTTAGGCTACCTTGCCTGTTGAGAAGCCTATTTAATCCTACAATTGAAATTCAACATGGATGACATCCCTGCACAAAGCATGGTGAGTTGTGAACACACACACACACACACACACACACACACCCCCAAAAAAAAAAAGAAGAAGAAAAGAGGAAAAAGGTCAAAGAGTTGGAATCTGCCTTGTGAAAAGCCTGTAGTTATAGACTTAACTTGTGATAATCCATTGTTTTAGTAGTAACTTGTACTACACTACCATGTAACCATCACTTCCTGATTTTGTTAGCTAGAATTGTTTGATACTCATATGACATACCACTAGTTGCTGTGTCTTTGTATTGTGATAACTTGGGAAGAAGGTTTTTAATATTGAACATGGTAATATAATTCTGCAATATCTTGGGC

Coding sequence (CDS)

ATGGCAACTCCACCACCACCGACGGCTCCGCCAGGGACCGGGAATTTCGAGGGGGGGCCGCTGCCAGCAGCGCCTCAGCCTCCGGGCACGGACATGACTGGAATTTGCTTTAGAGACCAGCTTTGGCTCAATACTTACCCTCTTGATCGAAACTTGGTTTTCGATTACTTCGCCTTGTCGCCTTTCTACGACTGGACTTGTAACAACGAACAACTCAGAATGCGCTCCATTCACCCCCTCGATCTTTCTCAACTCTCAAAAATGACAGGCATAGAGTACATGCTGAATGAAGTTATGGAGCCTCACCTTTTCGTTTTCAGAAAGCAAAAAAGGGATGGTCCTGAGAAAGTCACACCGATGCTGACTTATTATATCTTGGATGGTTCAATCTACCAGGCGCCTCAACTTTGCAGTGTTTTTGCTGCTCGCGTTAGTCGGGCACTGTATTACATATCAAAGGCCTTTACCACTGCTTCGTCAAAGTTGGAGAAGATAGGATATGTTGATTCTGAAAATGAGAGTGAAGAAGTAAAACCTGCAAAAGAAACCATTAACTTTAAGGAAGTAAAGCGAGTTGATCATATTCTTGCATCTTTACAGCGCAAGCTGCCACCTGCCCCACCACCCCCACCATTTCCTGAAGGTTATGCTCCTGCTGCCACAGCAGAGACAGAAAAAGGCCCTGAAAATCAGCAAGGAGAGTCACAACAGCCTTCTGCAGATCCCATTATTGATCAGGGTCCAGCTAAAAGGATGAAATTTTCAAGTGTTTAG

Protein sequence

MATPPPPTAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALSPFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPMLTYYILDGSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPAKETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSADPIIDQGPAKRMKFSSV*
Homology
BLAST of CSPI03G23680 vs. ExPASy Swiss-Prot
Match: F4IXJ7 (Mediator of RNA polymerase II transcription subunit 6 OS=Arabidopsis thaliana OX=3702 GN=MED6 PE=1 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 6.7e-86
Identity = 177/290 (61.03%), Postives = 195/290 (67.24%), Query Frame = 0

Query: 8   TAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALSPFYDWTC 67
           TA    GN     +P   QPPGTDMTGI FRDQLW+N+YPLDRN +FDYFALSPFYD TC
Sbjct: 10  TADTFNGN-AADQIPPPLQPPGTDMTGISFRDQLWINSYPLDRNYIFDYFALSPFYDTTC 69

Query: 68  NNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPMLTYYILD 127
           NNE LR RSIHPLDLS LSKMTG+EYML +  EP+LFVFRKQKRDGPEKVTPMLTYYILD
Sbjct: 70  NNEILRRRSIHPLDLSHLSKMTGLEYMLTDATEPNLFVFRKQKRDGPEKVTPMLTYYILD 129

Query: 128 GSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKI---------------------- 187
           GSIYQAPQLCSVFAARVSR +Y ISKAFT A+SKLE I                      
Sbjct: 130 GSIYQAPQLCSVFAARVSRTIYNISKAFTDAASKLETIRQDLQVCLVAIVLSLSVNLGSY 189

Query: 188 ---------------GY------VDSENESEEVKPAKETINFKEVKRVDHILASLQRKLP 247
                          G+       +++NE  E KPA ET++ KE+KRVD IL SL RKL 
Sbjct: 190 FLLIFKLMANGEQVYGFNKFLFDTENQNEPAESKPASETVDLKEMKRVDVILTSLYRKLA 249

Query: 248 PAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSADPIIDQGPAKRMKF 255
           P PPPPPFPEGY        ++    Q GESQ P  DPIIDQGPAKRMKF
Sbjct: 250 PPPPPPPFPEGYVSQEALGEKEELGTQGGESQPPQVDPIIDQGPAKRMKF 298

BLAST of CSPI03G23680 vs. ExPASy Swiss-Prot
Match: Q54PN3 (Putative mediator of RNA polymerase II transcription subunit 6 OS=Dictyostelium discoideum OX=44689 GN=med6 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 8.4e-28
Identity = 77/202 (38.12%), Postives = 114/202 (56.44%), Query Frame = 0

Query: 31  DMTGICFRDQLWLNTYPLDRNLVFDYFALSPFYDWTCNNEQLRMRSIHPLDLSQLSKMTG 90
           D+T + +RD LWL  YPL+   +  YF+ S FYD  CNNEQL+M+    LDLS L  M G
Sbjct: 65  DLTQVMWRDPLWLQMYPLNPQTILQYFSYSQFYDKNCNNEQLKMQR---LDLSALKNMDG 124

Query: 91  IEYMLNEVMEPHLFVFRKQKRDGPEKVTPMLTYYILDGSIYQAPQLCSVFAARVSRALYY 150
           +EY L + +EP  F+  KQ R  P  V     YY+++G+IYQAP+L  VF +RVS+++ +
Sbjct: 125 LEYELIKFVEPSFFLIAKQTRISPTDVLINTLYYVINGNIYQAPELHVVFKSRVSQSISH 184

Query: 151 ISKAFTTASSKLE---KIGY---VDSENESEEVK-PAKETINFKEVKRVDHILASLQRKL 210
           +S+AF + SS +      GY   +D  N+ E+ K  A      ++ KR+D ++ SL  K 
Sbjct: 185 LSEAFNSISSIVNWDIVNGYSLNLDPSNQQEKSKLAAYSRKQIEDTKRLDQLINSLFIKF 244

Query: 211 PPAPPPPPFPEGYAPAATAETE 226
           P    P   P+      T + +
Sbjct: 245 PAINRPVENPQQMGGGITPQQQ 263

BLAST of CSPI03G23680 vs. ExPASy Swiss-Prot
Match: Q921D4 (Mediator of RNA polymerase II transcription subunit 6 OS=Mus musculus OX=10090 GN=Med6 PE=1 SV=2)

HSP 1 Score: 111.7 bits (278), Expect = 1.3e-23
Identity = 87/241 (36.10%), Postives = 123/241 (51.04%), Query Frame = 0

Query: 31  DMTGICFRDQLWLNTYPLDRNLVFDYFA--LSPFYDWTCNNEQLRMRSIHPLDLSQLSKM 90
           ++ GI + D  W+    L+   V DYF+   +PFYD TCNNE ++M+    L L  L++M
Sbjct: 9   NLLGISWVDSSWIPI--LNSGSVLDYFSERSNPFYDRTCNNEVVKMQR---LTLEHLNQM 68

Query: 91  TGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPMLTYYILDGSIYQAPQLCSVFAARVSRAL 150
            GIEY+L    EP LF+ RKQ+R  P +V P+  YYI+ G IYQAP L SV  +RV  A+
Sbjct: 69  VGIEYILLHAQEPILFIIRKQQRQSPAQVIPLADYYIIAGVIYQAPDLGSVINSRVLTAV 128

Query: 151 YYISKAFTTASSKLE---KIGY---VDSENESEEVKP----AKETINFKEVKRVDHILAS 210
           + I  AF  A S        GY        E E+VKP     +E  +  + +RVD +L  
Sbjct: 129 HGIQSAFDEAMSYCRYHPSKGYWWHFKDHEEQEKVKPKAKRKEEPSSIFQRQRVDALLID 188

Query: 211 LQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSADPIIDQ------GPAKRM 254
           L++K PP        E   P   A+ E  P  +  +S++  +   I Q       P KRM
Sbjct: 189 LRQKFPPRFVQQKSGEKPVPVDQAKKEAEPLPETVKSEEKESTKNIQQTVSTKGPPEKRM 244

BLAST of CSPI03G23680 vs. ExPASy Swiss-Prot
Match: Q7Q107 (Mediator of RNA polymerase II transcription subunit 6 OS=Anopheles gambiae OX=7165 GN=MED6 PE=3 SV=3)

HSP 1 Score: 109.0 bits (271), Expect = 8.1e-23
Identity = 82/235 (34.89%), Postives = 118/235 (50.21%), Query Frame = 0

Query: 35  ICFRDQLWLNTYPLDRNLVFDYFA--LSPFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIE 94
           I + D  W+    L+   V DYF+   +PFYD TCNNE +RM+      L  L+ MTG+E
Sbjct: 19  ISWHDSNWIPV--LNPGNVMDYFSEKSNPFYDRTCNNEIVRMQR---QSLELLNNMTGVE 78

Query: 95  YMLNEVMEPHLFVFRKQKRDGPEKVTPMLTYYILDGSIYQAPQLCSVFAARVSRALYYIS 154
           Y+   V +P L+V RKQ R  P + TPM  YYI+ G++YQAP L SVF +R+   ++++ 
Sbjct: 79  YIPLHVQDPILYVIRKQHRHSPTEATPMADYYIIAGTVYQAPDLASVFNSRILSTVHHLQ 138

Query: 155 KAFTTASS--------------KLEKIGYVDSENESEEVKPAK-ETINFKEVKRVDHILA 214
            AF  ASS                 K     ++ ++++  P K E  +  + +RVD +L 
Sbjct: 139 TAFDEASSYSRYHPSKGYSWDFSSNKAIAEKTKTQTKKEAPVKEEPSSIFQRQRVDMLLG 198

Query: 215 SLQRKLP-PAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSAD-PIIDQGPAK 251
            L RK P P P     P G  P+ TA          G+S    AD  +I Q P +
Sbjct: 199 DLLRKFPLPLPQMTNNPTGGNPSDTANASNNHGGAAGDSDHVGADATLIKQEPTE 248

BLAST of CSPI03G23680 vs. ExPASy Swiss-Prot
Match: Q3SZY9 (Mediator of RNA polymerase II transcription subunit 6 OS=Bos taurus OX=9913 GN=MED6 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.4e-22
Identity = 83/241 (34.44%), Postives = 121/241 (50.21%), Query Frame = 0

Query: 31  DMTGICFRDQLWLNTYPLDRNLVFDYFA--LSPFYDWTCNNEQLRMRSIHPLDLSQLSKM 90
           ++ GI + D  W+    L+   V DYF+   +PFYD TCNNE ++M+    L L  L++M
Sbjct: 9   NLLGISWVDSSWIPI--LNSGSVLDYFSERSNPFYDRTCNNEVVKMQR---LTLEHLNQM 68

Query: 91  TGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPMLTYYILDGSIYQAPQLCSVFAARVSRAL 150
            G+EY+L    EP LF+ RKQ+R  P +V P+  YYI+ G IYQAP L SV  +RV  A+
Sbjct: 69  VGVEYILLHAQEPILFIIRKQQRQSPTQVIPLADYYIIAGVIYQAPDLGSVINSRVLTAV 128

Query: 151 YYISKAFTTASSKLE---KIGY---VDSENESEEVKP----AKETINFKEVKRVDHILAS 210
           + I  AF  A S        GY        E ++VKP     +E  +  + +RVD +L  
Sbjct: 129 HGIQSAFDEAMSYCRYHPSKGYWWHFKDHEEQDKVKPKVKRKEEPSSIFQRQRVDALLLD 188

Query: 211 LQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSADPIIDQ------GPAKRM 254
           L++K PP        E   P    + E  P  +  +S++      + Q       P KRM
Sbjct: 189 LRQKFPPKFVQQKSGEKPVPVDQTKKEAEPLPETVKSEEKETAKNVQQTVGTKGPPEKRM 244

BLAST of CSPI03G23680 vs. ExPASy TrEMBL
Match: A0A0A0L8C2 (Mediator of RNA polymerase II transcription subunit 6 OS=Cucumis sativus OX=3659 GN=Csa_3G434940 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 6.2e-143
Identity = 257/257 (100.00%), Postives = 257/257 (100.00%), Query Frame = 0

Query: 1   MATPPPPTAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS 60
           MATPPPPTAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS
Sbjct: 1   MATPPPPTAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS 60

Query: 61  PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPM 120
           PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPM
Sbjct: 61  PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPM 120

Query: 121 LTYYILDGSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPA 180
           LTYYILDGSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPA
Sbjct: 121 LTYYILDGSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPA 180

Query: 181 KETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSA 240
           KETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSA
Sbjct: 181 KETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSA 240

Query: 241 DPIIDQGPAKRMKFSSV 258
           DPIIDQGPAKRMKFSSV
Sbjct: 241 DPIIDQGPAKRMKFSSV 257

BLAST of CSPI03G23680 vs. ExPASy TrEMBL
Match: A0A5D3CDR8 (Mediator of RNA polymerase II transcription subunit 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold323G00160 PE=3 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.2e-138
Identity = 250/255 (98.04%), Postives = 253/255 (99.22%), Query Frame = 0

Query: 1   MATPPPP-TAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFAL 60
           MATPPPP TAPPGTGNFEGGP+PAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFAL
Sbjct: 1   MATPPPPTTAPPGTGNFEGGPMPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFAL 60

Query: 61  SPFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTP 120
           SPFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTP
Sbjct: 61  SPFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTP 120

Query: 121 MLTYYILDGSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKP 180
           MLTYYILDGSIYQAPQLC+VFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKP
Sbjct: 121 MLTYYILDGSIYQAPQLCNVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKP 180

Query: 181 AKETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPS 240
           AKETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPA TA+TEKGPENQQGESQQPS
Sbjct: 181 AKETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAPTADTEKGPENQQGESQQPS 240

Query: 241 ADPIIDQGPAKRMKF 255
           ADPIIDQGPAKRMKF
Sbjct: 241 ADPIIDQGPAKRMKF 255

BLAST of CSPI03G23680 vs. ExPASy TrEMBL
Match: A0A1S3BHR9 (Mediator of RNA polymerase II transcription subunit 6 OS=Cucumis melo OX=3656 GN=LOC103489986 PE=3 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.2e-138
Identity = 250/255 (98.04%), Postives = 253/255 (99.22%), Query Frame = 0

Query: 1   MATPPPP-TAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFAL 60
           MATPPPP TAPPGTGNFEGGP+PAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFAL
Sbjct: 1   MATPPPPTTAPPGTGNFEGGPMPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFAL 60

Query: 61  SPFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTP 120
           SPFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTP
Sbjct: 61  SPFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTP 120

Query: 121 MLTYYILDGSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKP 180
           MLTYYILDGSIYQAPQLC+VFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKP
Sbjct: 121 MLTYYILDGSIYQAPQLCNVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKP 180

Query: 181 AKETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPS 240
           AKETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPA TA+TEKGPENQQGESQQPS
Sbjct: 181 AKETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAPTADTEKGPENQQGESQQPS 240

Query: 241 ADPIIDQGPAKRMKF 255
           ADPIIDQGPAKRMKF
Sbjct: 241 ADPIIDQGPAKRMKF 255

BLAST of CSPI03G23680 vs. ExPASy TrEMBL
Match: A0A6J1D4P7 (Mediator of RNA polymerase II transcription subunit 6 OS=Momordica charantia OX=3673 GN=LOC111016983 PE=3 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.2e-133
Identity = 241/254 (94.88%), Postives = 242/254 (95.28%), Query Frame = 0

Query: 1   MATPPPPTAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS 60
           MATPPPP APPG  NFEGGP PAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS
Sbjct: 1   MATPPPPAAPPGIANFEGGPPPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS 60

Query: 61  PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPM 120
           PFYDWTCNNEQLRMRSIHPLDLSQLSKMTG EYMLNEVMEPHLFVFRKQKRDGPEKVTPM
Sbjct: 61  PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGTEYMLNEVMEPHLFVFRKQKRDGPEKVTPM 120

Query: 121 LTYYILDGSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPA 180
           LTYYILDGSIYQAPQLC+VFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKP 
Sbjct: 121 LTYYILDGSIYQAPQLCNVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPG 180

Query: 181 KETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSA 240
           KETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGY PA TAE EKGPENQ  E QQPSA
Sbjct: 181 KETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYVPAPTAEVEKGPENQPAELQQPSA 240

Query: 241 DPIIDQGPAKRMKF 255
           DPIIDQGPAKRMKF
Sbjct: 241 DPIIDQGPAKRMKF 254

BLAST of CSPI03G23680 vs. ExPASy TrEMBL
Match: A0A6J1G911 (Mediator of RNA polymerase II transcription subunit 6 OS=Cucurbita moschata OX=3662 GN=LOC111452031 PE=3 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 5.1e-129
Identity = 236/254 (92.91%), Postives = 241/254 (94.88%), Query Frame = 0

Query: 1   MATPPPPTAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS 60
           MATPP    PPG GNFEGGP  AAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS
Sbjct: 1   MATPP----PPGIGNFEGGPPLAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS 60

Query: 61  PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPM 120
           PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPM
Sbjct: 61  PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPM 120

Query: 121 LTYYILDGSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPA 180
           LTYYILDGSIYQAPQLC+VFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPA
Sbjct: 121 LTYYILDGSIYQAPQLCNVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPA 180

Query: 181 KETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSA 240
           KETI+FKEVKRVD ILASLQRKLPPAPPPPPFPEGY PA  +E EKGPE+QQGE QQP+A
Sbjct: 181 KETIDFKEVKRVDRILASLQRKLPPAPPPPPFPEGYVPAPASEAEKGPESQQGELQQPAA 240

Query: 241 DPIIDQGPAKRMKF 255
           DPIIDQGPAKRMKF
Sbjct: 241 DPIIDQGPAKRMKF 250

BLAST of CSPI03G23680 vs. NCBI nr
Match: XP_004149176.2 (mediator of RNA polymerase II transcription subunit 6 [Cucumis sativus] >KGN58013.1 hypothetical protein Csa_011693 [Cucumis sativus])

HSP 1 Score: 516.5 bits (1329), Expect = 1.3e-142
Identity = 257/257 (100.00%), Postives = 257/257 (100.00%), Query Frame = 0

Query: 1   MATPPPPTAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS 60
           MATPPPPTAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS
Sbjct: 1   MATPPPPTAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS 60

Query: 61  PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPM 120
           PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPM
Sbjct: 61  PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPM 120

Query: 121 LTYYILDGSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPA 180
           LTYYILDGSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPA
Sbjct: 121 LTYYILDGSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPA 180

Query: 181 KETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSA 240
           KETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSA
Sbjct: 181 KETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSA 240

Query: 241 DPIIDQGPAKRMKFSSV 258
           DPIIDQGPAKRMKFSSV
Sbjct: 241 DPIIDQGPAKRMKFSSV 257

BLAST of CSPI03G23680 vs. NCBI nr
Match: XP_008447572.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 6 isoform X1 [Cucumis melo] >KAA0050863.1 mediator of RNA polymerase II transcription subunit 6 isoform X1 [Cucumis melo var. makuwa] >TYK08489.1 mediator of RNA polymerase II transcription subunit 6 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 502.3 bits (1292), Expect = 2.5e-138
Identity = 250/255 (98.04%), Postives = 253/255 (99.22%), Query Frame = 0

Query: 1   MATPPPP-TAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFAL 60
           MATPPPP TAPPGTGNFEGGP+PAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFAL
Sbjct: 1   MATPPPPTTAPPGTGNFEGGPMPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFAL 60

Query: 61  SPFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTP 120
           SPFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTP
Sbjct: 61  SPFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTP 120

Query: 121 MLTYYILDGSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKP 180
           MLTYYILDGSIYQAPQLC+VFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKP
Sbjct: 121 MLTYYILDGSIYQAPQLCNVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKP 180

Query: 181 AKETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPS 240
           AKETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPA TA+TEKGPENQQGESQQPS
Sbjct: 181 AKETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAPTADTEKGPENQQGESQQPS 240

Query: 241 ADPIIDQGPAKRMKF 255
           ADPIIDQGPAKRMKF
Sbjct: 241 ADPIIDQGPAKRMKF 255

BLAST of CSPI03G23680 vs. NCBI nr
Match: XP_038896808.1 (mediator of RNA polymerase II transcription subunit 6 [Benincasa hispida])

HSP 1 Score: 498.0 bits (1281), Expect = 4.7e-137
Identity = 247/254 (97.24%), Postives = 248/254 (97.64%), Query Frame = 0

Query: 1   MATPPPPTAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS 60
           MATPPPPTAPPG GNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS
Sbjct: 1   MATPPPPTAPPGIGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS 60

Query: 61  PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPM 120
           PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPM
Sbjct: 61  PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPM 120

Query: 121 LTYYILDGSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPA 180
           LTYYILDGSIYQAPQLC+VFAARVSRALYYISKAFTTASSKLEKIGYVDSENE EEVKPA
Sbjct: 121 LTYYILDGSIYQAPQLCNVFAARVSRALYYISKAFTTASSKLEKIGYVDSENEGEEVKPA 180

Query: 181 KETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSA 240
           KETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPA TAETEKGPEN  GESQQP A
Sbjct: 181 KETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAPTAETEKGPENPLGESQQPPA 240

Query: 241 DPIIDQGPAKRMKF 255
           DPIIDQGPAKRMKF
Sbjct: 241 DPIIDQGPAKRMKF 254

BLAST of CSPI03G23680 vs. NCBI nr
Match: XP_022148297.1 (mediator of RNA polymerase II transcription subunit 6 [Momordica charantia])

HSP 1 Score: 485.7 bits (1249), Expect = 2.4e-133
Identity = 241/254 (94.88%), Postives = 242/254 (95.28%), Query Frame = 0

Query: 1   MATPPPPTAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS 60
           MATPPPP APPG  NFEGGP PAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS
Sbjct: 1   MATPPPPAAPPGIANFEGGPPPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS 60

Query: 61  PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPM 120
           PFYDWTCNNEQLRMRSIHPLDLSQLSKMTG EYMLNEVMEPHLFVFRKQKRDGPEKVTPM
Sbjct: 61  PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGTEYMLNEVMEPHLFVFRKQKRDGPEKVTPM 120

Query: 121 LTYYILDGSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPA 180
           LTYYILDGSIYQAPQLC+VFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKP 
Sbjct: 121 LTYYILDGSIYQAPQLCNVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPG 180

Query: 181 KETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSA 240
           KETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGY PA TAE EKGPENQ  E QQPSA
Sbjct: 181 KETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYVPAPTAEVEKGPENQPAELQQPSA 240

Query: 241 DPIIDQGPAKRMKF 255
           DPIIDQGPAKRMKF
Sbjct: 241 DPIIDQGPAKRMKF 254

BLAST of CSPI03G23680 vs. NCBI nr
Match: XP_023524903.1 (mediator of RNA polymerase II transcription subunit 6 [Cucurbita pepo subsp. pepo] >XP_023524905.1 mediator of RNA polymerase II transcription subunit 6 [Cucurbita pepo subsp. pepo] >KAG7036453.1 Mediator of RNA polymerase II transcription subunit 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 471.5 bits (1212), Expect = 4.7e-129
Identity = 236/254 (92.91%), Postives = 241/254 (94.88%), Query Frame = 0

Query: 1   MATPPPPTAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS 60
           MATPP    PPG GNFEGGP PAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS
Sbjct: 1   MATPP----PPGIGNFEGGPPPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALS 60

Query: 61  PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPM 120
           PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPM
Sbjct: 61  PFYDWTCNNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPM 120

Query: 121 LTYYILDGSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPA 180
           LTYYILDGSIYQAPQLC+VFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPA
Sbjct: 121 LTYYILDGSIYQAPQLCNVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESEEVKPA 180

Query: 181 KETINFKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSA 240
           K TI+FKEVKRVD ILASLQRKLPPAPPPPPFPEGY PA  +E EKGPE+QQGE QQP+A
Sbjct: 181 KGTIDFKEVKRVDRILASLQRKLPPAPPPPPFPEGYVPAPASEAEKGPESQQGELQQPAA 240

Query: 241 DPIIDQGPAKRMKF 255
           DPIIDQGPAKRMKF
Sbjct: 241 DPIIDQGPAKRMKF 250

BLAST of CSPI03G23680 vs. TAIR 10
Match: AT3G21350.1 (RNA polymerase transcriptional regulation mediator-related )

HSP 1 Score: 340.1 bits (871), Expect = 1.5e-93
Identity = 179/249 (71.89%), Postives = 196/249 (78.71%), Query Frame = 0

Query: 8   TAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALSPFYDWTC 67
           TA    GN     +P   QPPGTDMTGI FRDQLW+N+YPLDRN +FDYFALSPFYD TC
Sbjct: 10  TADTFNGN-AADQIPPPLQPPGTDMTGISFRDQLWINSYPLDRNYIFDYFALSPFYDTTC 69

Query: 68  NNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPMLTYYILD 127
           NNE LR RSIHPLDLS LSKMTG+EYML +  EP+LFVFRKQKRDGPEKVTPMLTYYILD
Sbjct: 70  NNEILRRRSIHPLDLSHLSKMTGLEYMLTDATEPNLFVFRKQKRDGPEKVTPMLTYYILD 129

Query: 128 GSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKIGYVDSENESE--EVKPAKETIN 187
           GSIYQAPQLCSVFAARVSR +Y ISKAFT A+SKLE I  VD+EN++E  E KPA ET++
Sbjct: 130 GSIYQAPQLCSVFAARVSRTIYNISKAFTDAASKLETIRQVDTENQNEPAESKPASETVD 189

Query: 188 FKEVKRVDHILASLQRKLPPAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSADPIID 247
            KE+KRVD IL SL RKL P PPPPPFPEGY        ++    Q GESQ P  DPIID
Sbjct: 190 LKEMKRVDVILTSLYRKLAPPPPPPPFPEGYVSQEALGEKEELGTQGGESQPPQVDPIID 249

Query: 248 QGPAKRMKF 255
           QGPAKRMKF
Sbjct: 250 QGPAKRMKF 257

BLAST of CSPI03G23680 vs. TAIR 10
Match: AT3G21350.2 (RNA polymerase transcriptional regulation mediator-related )

HSP 1 Score: 318.5 bits (815), Expect = 4.8e-87
Identity = 177/290 (61.03%), Postives = 195/290 (67.24%), Query Frame = 0

Query: 8   TAPPGTGNFEGGPLPAAPQPPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALSPFYDWTC 67
           TA    GN     +P   QPPGTDMTGI FRDQLW+N+YPLDRN +FDYFALSPFYD TC
Sbjct: 10  TADTFNGN-AADQIPPPLQPPGTDMTGISFRDQLWINSYPLDRNYIFDYFALSPFYDTTC 69

Query: 68  NNEQLRMRSIHPLDLSQLSKMTGIEYMLNEVMEPHLFVFRKQKRDGPEKVTPMLTYYILD 127
           NNE LR RSIHPLDLS LSKMTG+EYML +  EP+LFVFRKQKRDGPEKVTPMLTYYILD
Sbjct: 70  NNEILRRRSIHPLDLSHLSKMTGLEYMLTDATEPNLFVFRKQKRDGPEKVTPMLTYYILD 129

Query: 128 GSIYQAPQLCSVFAARVSRALYYISKAFTTASSKLEKI---------------------- 187
           GSIYQAPQLCSVFAARVSR +Y ISKAFT A+SKLE I                      
Sbjct: 130 GSIYQAPQLCSVFAARVSRTIYNISKAFTDAASKLETIRQDLQVCLVAIVLSLSVNLGSY 189

Query: 188 ---------------GY------VDSENESEEVKPAKETINFKEVKRVDHILASLQRKLP 247
                          G+       +++NE  E KPA ET++ KE+KRVD IL SL RKL 
Sbjct: 190 FLLIFKLMANGEQVYGFNKFLFDTENQNEPAESKPASETVDLKEMKRVDVILTSLYRKLA 249

Query: 248 PAPPPPPFPEGYAPAATAETEKGPENQQGESQQPSADPIIDQGPAKRMKF 255
           P PPPPPFPEGY        ++    Q GESQ P  DPIIDQGPAKRMKF
Sbjct: 250 PPPPPPPFPEGYVSQEALGEKEELGTQGGESQPPQVDPIIDQGPAKRMKF 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4IXJ76.7e-8661.03Mediator of RNA polymerase II transcription subunit 6 OS=Arabidopsis thaliana OX... [more]
Q54PN38.4e-2838.12Putative mediator of RNA polymerase II transcription subunit 6 OS=Dictyostelium ... [more]
Q921D41.3e-2336.10Mediator of RNA polymerase II transcription subunit 6 OS=Mus musculus OX=10090 G... [more]
Q7Q1078.1e-2334.89Mediator of RNA polymerase II transcription subunit 6 OS=Anopheles gambiae OX=71... [more]
Q3SZY92.4e-2234.44Mediator of RNA polymerase II transcription subunit 6 OS=Bos taurus OX=9913 GN=M... [more]
Match NameE-valueIdentityDescription
A0A0A0L8C26.2e-143100.00Mediator of RNA polymerase II transcription subunit 6 OS=Cucumis sativus OX=3659... [more]
A0A5D3CDR81.2e-13898.04Mediator of RNA polymerase II transcription subunit 6 OS=Cucumis melo var. makuw... [more]
A0A1S3BHR91.2e-13898.04Mediator of RNA polymerase II transcription subunit 6 OS=Cucumis melo OX=3656 GN... [more]
A0A6J1D4P71.2e-13394.88Mediator of RNA polymerase II transcription subunit 6 OS=Momordica charantia OX=... [more]
A0A6J1G9115.1e-12992.91Mediator of RNA polymerase II transcription subunit 6 OS=Cucurbita moschata OX=3... [more]
Match NameE-valueIdentityDescription
XP_004149176.21.3e-142100.00mediator of RNA polymerase II transcription subunit 6 [Cucumis sativus] >KGN5801... [more]
XP_008447572.12.5e-13898.04PREDICTED: mediator of RNA polymerase II transcription subunit 6 isoform X1 [Cuc... [more]
XP_038896808.14.7e-13797.24mediator of RNA polymerase II transcription subunit 6 [Benincasa hispida][more]
XP_022148297.12.4e-13394.88mediator of RNA polymerase II transcription subunit 6 [Momordica charantia][more]
XP_023524903.14.7e-12992.91mediator of RNA polymerase II transcription subunit 6 [Cucurbita pepo subsp. pep... [more]
Match NameE-valueIdentityDescription
AT3G21350.11.5e-9371.89RNA polymerase transcriptional regulation mediator-related [more]
AT3G21350.24.8e-8761.03RNA polymerase transcriptional regulation mediator-related [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007018Mediator complex, subunit Med6PFAMPF04934Med6coord: 35..160
e-value: 7.3E-41
score: 139.0
IPR007018Mediator complex, subunit Med6PANTHERPTHR13104MED-6-RELATEDcoord: 20..254
IPR016820Mediator complex, subunit Med6, metazoa/plantPIRSFPIRSF023869MED6_meta/plantcoord: 20..254
e-value: 1.2E-88
score: 294.5
IPR038566Mediator complex, subunit Med6 superfamilyGENE3D3.10.450.580Mediator complex, subunit Med6coord: 29..158
e-value: 1.8E-44
score: 153.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 199..257
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 225..242
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 202..216
NoneNo IPR availablePANTHERPTHR13104:SF2MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 6coord: 20..254

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G23680.1CSPI03G23680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045893 positive regulation of transcription, DNA-templated
biological_process GO:0006357 regulation of transcription by RNA polymerase II
cellular_component GO:0070847 core mediator complex
cellular_component GO:0016592 mediator complex
molecular_function GO:0003713 transcription coactivator activity
molecular_function GO:0003712 transcription coregulator activity