CSPI03G22270 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G22270
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPyridoxal kinase
LocationChr3: 18717147 .. 18722461 (-)
RNA-Seq ExpressionCSPI03G22270
SyntenyCSPI03G22270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGGGCAGAGGCATTTTGGTAGTTTCATCCCATCTCTTCCAAATCTCCTACATAATTTCTGTCTCTACTTACGATCACTTTTCACTTCCCTCCCCCACCACCTCCCTCATCGTTGCTTCTTTTGCATTATTAAAACTTCCTCACTTTTCAGTTTCTTTCATTTTTCCTCAACAAAATTCTCTCTCAACTCTCATTTTCCTCCACCAATCTCTTCTTTCACGCATTCCGGTTTGTTGATTTCTTTAATCTCCACTTCTTCAGGCCTTCCGTAACGAATGTCGCCCCCAATCCTCTCCTTAGCTCTCCCGTCCCCCACTGGTCGAGTTCTCAGTATTCAGTCCCACACTGTTCAGGTTTGTTCAATACTTCACTACGGTTCCGGTTATTTTGGTACAATTTCATTTCAAACTCCATATTCACTTTGTTTTTGTTTCTTTCATTAGGCTTCTGTAGGAGTTTCCTTCAGTTCTGTTGCCAATTTGTATTGTTATATACTTAGACTTGGTGTTTTTGTTATAAGTGCTGTGTTTGTGGGAGGTTTGACTTTATATTTACTATCTTGATAATGATGTCTGATGTTACTTTTCTGCTTTGTGTATTTCTGTGGATTGTTTGTTCTTATATTGTTCTTGATTGGCTTTATTTCCGTGCTATTAGTTTTACTTCAATGGATTGTTCTATTGTCCGCTGCCGTAGGGATACGTTGGGAATAAATCAGCTGTTTTCCCTCTCCAACTATTGGGCTATGATGTAGATCCAATAAATTCCGTGCAGTTCTCAAACCACACAGGCAAGATGCTTCTATTTGTTCATTCAAATGGTCTTAGAACTCTGAGGCTTAATTATATAGGTGCCTTATGTCCGTATGCATAGGTTCTGGTTTGATTTTGTTGTGTTAGTGAAAGTGTATTATGGTTCCTTTTGAAAATAAAACAGGGTATCCGACTTTTAAGGGCCAAGTTTTGAATGGAAGACAATTATGGGACTTAATTGAAGGCCTTGAAGAAAATGAATTGTTGTACTATACTCATTTGTTAACAGGTACTTTAGTTTTTCACTAAGTGACTTTTGGTGTGGAGTATAATTTGGTGACTTTGCAATATGAGGACCTATGAATTGTATGTTGGCAGAAAATAATAAATGTCTGCTAGAAAAGGAGAACTATGCTAAACTATTGCCAGCATCACGTATAAAGCTCCTGCAGTAATTTGCATACTGTGAACATGCCTATTTTTATTTTTCAGTTAATGTGAGATTTGTTGTAATATGGTACCTATGGATTTTGCAGGATATATTGGTTCTGTTTCTTTCCTCAACACAGTGTTGGAAGTTGTCGATAAGCTTCGCTTGGTGAACCCTAAGCTAACATATGGTCAGATAAATTCAAGTTCATGGTGCTTCATTATCATTTGTCCATAATCATATATAGGTTTTGTAAATGTTTTCTTCGAGCTCTTGGTAACCTAGAACTGGTGAGCTGGGAGGATAATGTTTTCCCAGGAAAAAAAGCTGGGAGAATAATAATTTTCTAATGTGTTTTTGCACGCAATGGACAATCTTGTCCATGATTGATGATTCATTATCTTCAAGTCCAGAACTTCTTATATTGCCTTTGTTCCATGCATATAACGTATATGCTAGGGAAACAGAAACATTGTAATTTATAATGTGGAAAATTTTGAGGATTAGTCAACCACCTATTTAACCATCTCATTTTTTCTTTTTTGAAACGGAGACAAGCCTCTTAAATTATTATTAATAAAAGAGCCTTAAGCTCAAAGTACAAGAGTATTACACTAAGAGCAAGAGAAAGGAGAAAATTCAGTCTACATCTACAACACAAGCTAAAAAACAAATTCCAAATAGAAGAGATCAAGCTAAACAAAACCAACTCATTAAAACTAATGTTTTTTCCAGTATGTGATCCAGTGATGGGCGATGAAGGGAAGCTTTATGTTCCTGAAGAACTAGTATCTGTATACCGAGAGAAGGTGAGAAATCCAGCTCACAATGGCCCCTGGTAGTGCTTGATAGCTCATTCACTTAATCTTGACTTGACTTAGATTTTTCCTTAATTAGCATTGTCTGTTGAACTTTTCAATGTTTGAGTACTTTAGTATGTGGACCATTAAAAATGTAATTCTGTATCTCTGTGTGTTGGAAGCTAACAATTATTTATGCATCTGCCCTTTATATTTTCATTTTCATGTTCTTGTCCATAAATTTTTTTGATAAGTCCAATTCATGATTAGGTAATACCGGTGGCTTCAGTGTTGACTCCTAATCAATTTGAAGCTGAACAACTGACTGGGTTAAGGTAATTTGAATAGGATAGACATGATGTGCTAATGTTATTTTTACTGGTCTATGTAGATATATGGAAAGCGTATAACACACGTTTACTTTTGTCTTTTTGAATGTGTGCATGTGTTGGTGAAGTGCAAGAAAAGATACCTTTTAAACCTTATTAGTGTTTTACTGTATGATGTCTATACTTAAATCATGAAATTAAGTGCAAACTGGTATGGTTGGAAGTGGAGACTGGGGTTCCTTGTCTTTTTGATCGAGCCAAATTTGATACACCCTTTCTTGAATTCTTCTGAGTTTTTCTCCCACCTTCTGATCTTATTATTTTAGATTTCTCTATGTAATTCTATATAACAATTAGCTGTTTTTCTTTAAAACTCTAGAATTTTTAGTTTAAGTGAACCTCAATTTGTCTGATTTGCAAGTAGTTGGTACTTCAGCATCCTTTTCTTCCTTGTCAGATAGAGTGATTTTTGAGAATCCTTGCTTTTTGCAGGATCCAATCTGAAGGAGATGGTAGGGAAGCTTGTAACATTCTTCATGCTGCAGGACCTTCAAAGGTAAGAATTTCGAATGTGGCAATTGGCCTGCTGAGAAATTGATGGGCATAGGTATTTCTTTTCTTAAGGGTGCTTGTTAGTAGTCAGCCATTGTGAATTGGAGACATTGGAGGTCAATGAACTATTGTAGGAGTGATATCTTACAAGTGAATTTCACGTTCACAACTTGTGAAAGGTAGATGGCAATCGACACATTAGATGCCTGTCTCTGTTAGATAATTGATGCATCACAACCATAGTTTGGCTGTATGAATTGGATCGTGCAACAAAATAGTTGGAGGCCTGCGTACAATAATAGTCAATAAGATTTAATTACTGATGAGTTACGGATTTAGTTTGGAAGTAGTTTAAACATCTTCTGTTTTCCAGGTTTTCCAGACACTGTGTTGAGACAAAATTTCAATTGTAGGTGCTTATCCTTTTAAGAATTCCAATTTCTACAGCTCTAGTCCTAATTACTTAAGGCGGTTAGAACTTCTCCCAGCTTAGGGTACCTAGCTGTCGAACTGTGTTTTACATTTTAATATAATCCTAGCTCCTTTGGTAAACATAAATACGATCCATTATGTTACTGTCAACCTTTTTTGTAAAAATGTCAGACATCATTCTGAGTATTATTTCATGCATTGAATAACTCTGTTTTCTGATGATTAAGTCATGACAAACACCCCAGGTTGTGATTACGAGTATAAATATGAACGGTGAACTCCTTCTCATTGGCAGTCATCAGAAGAATGAGGTGAACTTTTGCATTAAGCTCTTGTAGTTTGTCTACAAAAAGACCCGAGTCATTTGGTTGATTCTTTTGTCTTCTTAAACTCCAAGCAAATTGTTTATGCATATGAGGTTAGATTCGCAAATAGACTTCATCATTATCACTGTCTGAATATTACCGAATAAGCTGATGTACCAAGTGTACGCTACTTTCCTAATCCTAGGTAACCTGTGCTTATGATGCTATGCTACACAGTATTCCAATCCTCCATTACTAGACATGGCAAAAGTTGAGACTGAAAAGAACCATTTCACTAAATAAGTTTATTTAGGTTAAAATATTATTTTACTTCCTGTGCTTTGAGTTTCATTGCTTCTTGAAACAACAATCTAAAATGCATTTACAACTTCCCTCTGTAATAAGCAAAATTTACTACTTTCTTTCTTTCTTTTTCTTTTAATTTTTTTTTATAATAGAGGTACAAATTCTTTTCAACTAAGATACAGTTGACAAGACTTTAGATTTAAGTTGAGCTCAGATAACTGAAATTTTGAAAGGACCATACTTTATTCCATCCATCCGACGTACGTTATTGGCATGTGAATGGTTCAGGAATTCATGTCCCTAGACTGAAAATTCATGGATCAACTCAAAAGAGAATGTGCTGTTGTATGGCTAAGAAAAAACCTACAAGAGGGAAGGAACCCTTACTGAAACTGTGTATTTTGATCTTAGAAAAGTTGTAACACTTAATGGTTTCTGGCCCTAAAATGATTCATCATAGTAGGGTCAGTCTTCATTCTTCAAGCTGCACGAACCCTATTTTACTCATGTAGACCTTTATTCACCTTTGCTCAAAAGATTTTTTGAGGATTGATTATTCGTATTCCGAGATGTCTATTTCAGTCTTTTATGACGATTAAATAAAACCAAAAAAGTCCCACGAAATTTTTGAATCTTTAACTCATAGCGGATGGTTGGGAAGGTTTTGTGGATTATGCAATGATTGACCATAAGAAAGCTTGGTATCGTGAAAGCTCCTACTGAAATGAAGAAACGAGCCTTTTCCCTACCTGAGATTCCCCTCCCCTTCATTTCATCCACCTTATTGTCTCTTTCACTACAAGCCCATTTCTGTAATGAAAATTGGTTGATTATAAAGTTGTCGGGCTGCATGGTTCCTTCTTTTGGGATTTAGGAAGCAGTACTTCATTTCCGTTATACATAGCCTTTTTTTTTTGTTGCATGAAGGGAGAAAACTGATTTTATATTTATTATTTGATGCTTTACAGTTGTATACGACACATTTTGTAGCCTTATGGTATGCCACTCTTATATGGGGAGCTGTTGTATTTTTTTAGTTTTATTCTCTGCCTTTGGTTTGATAGCTCGCTACTTTTTGTTTTTTGATCGTTATTAGGTCGTTTTCATGTTTGTGTTGCCTGGGTATATCTATTGTTTCAGTTGTATGCTGTATTTTCCTTAGTTCTTTTGCTTTTAGTATAATTCTCTTATACTTTGAATTTATTATTATTAATAAAGAAGTTTGTCTCCGTTTCAAAAAAATGCGCATGATCTAATTGCAGTAATTAAACCCTGATGTCGTAGGGCCAGGCTCCTGAACAATTTAAGATCATGATTCCCAAGATTCCAGCATATTTCACGGTAAAAAACCTCTGATCTAATTTCACTTGCAATGTTCATCTCTAACTTTTTTCAGCTGGACTGAGATA

mRNA sequence

CTGGGCAGAGGCATTTTGGTAGTTTCATCCCATCTCTTCCAAATCTCCTACATAATTTCTGTCTCTACTTACGATCACTTTTCACTTCCCTCCCCCACCACCTCCCTCATCGTTGCTTCTTTTGCATTATTAAAACTTCCTCACTTTTCAGTTTCTTTCATTTTTCCTCAACAAAATTCTCTCTCAACTCTCATTTTCCTCCACCAATCTCTTCTTTCACGCATTCCGGTTTGTTGATTTCTTTAATCTCCACTTCTTCAGGCCTTCCGTAACGAATGTCGCCCCCAATCCTCTCCTTAGCTCTCCCGTCCCCCACTGGTCGAGTTCTCAGTATTCAGTCCCACACTGTTCAGGGATACGTTGGGAATAAATCAGCTGTTTTCCCTCTCCAACTATTGGGCTATGATGTAGATCCAATAAATTCCGTGCAGTTCTCAAACCACACAGGGTATCCGACTTTTAAGGGCCAAGTTTTGAATGGAAGACAATTATGGGACTTAATTGAAGGCCTTGAAGAAAATGAATTGTTGTACTATACTCATTTGTTAACAGGATATATTGGTTCTGTTTCTTTCCTCAACACAGTGTTGGAAGTTGTCGATAAGCTTCGCTTGGTGAACCCTAAGCTAACATATGTATGTGATCCAGTGATGGGCGATGAAGGGAAGCTTTATGTTCCTGAAGAACTAGTATCTGTATACCGAGAGAAGGTAATACCGGTGGCTTCAGTGTTGACTCCTAATCAATTTGAAGCTGAACAACTGACTGGGTTAAGGATCCAATCTGAAGGAGATGGTAGGGAAGCTTGTAACATTCTTCATGCTGCAGGACCTTCAAAGGTTGTGATTACGAGTATAAATATGAACGGTGAACTCCTTCTCATTGGCAGTCATCAGAAGAATGAGGGCCAGGCTCCTGAACAATTTAAGATCATGATTCCCAAGATTCCAGCATATTTCACGGTAAAAAACCTCTGATCTAATTTCACTTGCAATGTTCATCTCTAACTTTTTTCAGCTGGACTGAGATA

Coding sequence (CDS)

ATGTCGCCCCCAATCCTCTCCTTAGCTCTCCCGTCCCCCACTGGTCGAGTTCTCAGTATTCAGTCCCACACTGTTCAGGGATACGTTGGGAATAAATCAGCTGTTTTCCCTCTCCAACTATTGGGCTATGATGTAGATCCAATAAATTCCGTGCAGTTCTCAAACCACACAGGGTATCCGACTTTTAAGGGCCAAGTTTTGAATGGAAGACAATTATGGGACTTAATTGAAGGCCTTGAAGAAAATGAATTGTTGTACTATACTCATTTGTTAACAGGATATATTGGTTCTGTTTCTTTCCTCAACACAGTGTTGGAAGTTGTCGATAAGCTTCGCTTGGTGAACCCTAAGCTAACATATGTATGTGATCCAGTGATGGGCGATGAAGGGAAGCTTTATGTTCCTGAAGAACTAGTATCTGTATACCGAGAGAAGGTAATACCGGTGGCTTCAGTGTTGACTCCTAATCAATTTGAAGCTGAACAACTGACTGGGTTAAGGATCCAATCTGAAGGAGATGGTAGGGAAGCTTGTAACATTCTTCATGCTGCAGGACCTTCAAAGGTTGTGATTACGAGTATAAATATGAACGGTGAACTCCTTCTCATTGGCAGTCATCAGAAGAATGAGGGCCAGGCTCCTGAACAATTTAAGATCATGATTCCCAAGATTCCAGCATATTTCACGGTAAAAAACCTCTGA

Protein sequence

MSPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYPTFKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTYVCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNILHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFTVKNL*
Homology
BLAST of CSPI03G22270 vs. ExPASy Swiss-Prot
Match: Q8W1X2 (Pyridoxal kinase OS=Arabidopsis thaliana OX=3702 GN=PK PE=1 SV=2)

HSP 1 Score: 398.3 bits (1022), Expect = 6.1e-110
Identity = 195/228 (85.53%), Postives = 212/228 (92.98%), Query Frame = 0

Query: 2   SPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYPT 61
           +PP+LSLALPS TGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYPT
Sbjct: 3   TPPVLSLALPSDTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYPT 62

Query: 62  FKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTYV 121
           FKGQVLNG+QL DLIEGLE N+LL+YTH+LTGYIGSVSFL+T+LEV++KLR VNP LTYV
Sbjct: 63  FKGQVLNGQQLCDLIEGLEANDLLFYTHVLTGYIGSVSFLDTILEVINKLRSVNPNLTYV 122

Query: 122 CDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNIL 181
           CDPVMGDEGKLYVPEELV VYREKV+P+AS+LTPNQFEAE+LTGLRI SE DGREAC IL
Sbjct: 123 CDPVMGDEGKLYVPEELVHVYREKVVPLASMLTPNQFEAEKLTGLRINSEEDGREACAIL 182

Query: 182 HAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT 230
           HAAGPSKVVITSI + G LLLIGSHQK +G  PEQFKI+I KIPAYFT
Sbjct: 183 HAAGPSKVVITSITIGGILLLIGSHQKEKGLKPEQFKILIHKIPAYFT 230

BLAST of CSPI03G22270 vs. ExPASy Swiss-Prot
Match: O46560 (Pyridoxal kinase OS=Sus scrofa OX=9823 GN=PDXK PE=1 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.4e-58
Identity = 118/225 (52.44%), Postives = 156/225 (69.33%), Query Frame = 0

Query: 16  RVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYPTFKGQVLNGRQLWDL 75
           RVLSIQSH V+GYVGN++A FPLQ+LG++VD +NSVQFSNHTGY  +KGQVLN  +L  L
Sbjct: 16  RVLSIQSHVVRGYVGNRAATFPLQVLGFEVDAVNSVQFSNHTGYAHWKGQVLNSDELHAL 75

Query: 76  IEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTYVCDPVMGD----EGK 135
            EGL+ N +  Y ++LTGY    SFL  V+++V +L+  NP+L YVCDPVMGD    EG 
Sbjct: 76  YEGLKLNNVNQYDYVLTGYTRDKSFLAMVVDIVRELKQQNPRLVYVCDPVMGDKWDGEGS 135

Query: 136 LYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNILHAAGPSKVVI 195
           +YVPE+L+ VYREKV+PVA ++TPNQFEAE LTG RI SE +     ++LHA GP  VVI
Sbjct: 136 MYVPEDLLPVYREKVVPVADIITPNQFEAELLTGRRIHSEEEALAVMDMLHAMGPDTVVI 195

Query: 196 TSINM-----NGELLLIGSHQKNE---GQAPEQFKIMIPKIPAYF 229
           TS ++        L+ +GS +        A ++ ++ I K+ A F
Sbjct: 196 TSSDLPSPRGKDYLIALGSQRTRSPDGSVATQRIRMEICKVDAVF 240

BLAST of CSPI03G22270 vs. ExPASy Swiss-Prot
Match: P82197 (Pyridoxal kinase OS=Ovis aries OX=9940 GN=PDXK PE=1 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.6e-57
Identity = 107/185 (57.84%), Postives = 142/185 (76.76%), Query Frame = 0

Query: 16  RVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYPTFKGQVLNGRQLWDL 75
           RVLSIQSH V+GYVGN++A FPLQ+LG++VD +NSVQFSNHTGY  +KGQVLN  +L +L
Sbjct: 6   RVLSIQSHVVRGYVGNRAATFPLQVLGFEVDAVNSVQFSNHTGYSHWKGQVLNSDELQEL 65

Query: 76  IEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTYVCDPVMGD----EGK 135
            +GL+ N +  Y ++LTGY    SFL  V+++V +L+  NP+L YVCDPVMGD    EG 
Sbjct: 66  YDGLKLNHVNQYDYVLTGYTRDKSFLAMVVDIVQELKQQNPRLVYVCDPVMGDQRNGEGA 125

Query: 136 LYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNILHAAGPSKVVI 195
           +YVP++L+ VYREKV+PVA ++TPNQFEAE LTG +I S+ +  E  ++LH+ GP  VVI
Sbjct: 126 MYVPDDLLPVYREKVVPVADIITPNQFEAELLTGRKIHSQEEALEVMDMLHSMGPDTVVI 185

Query: 196 TSINM 197
           TS N+
Sbjct: 186 TSSNL 190

BLAST of CSPI03G22270 vs. ExPASy Swiss-Prot
Match: Q0II59 (Pyridoxal kinase OS=Bos taurus OX=9913 GN=PDXK PE=2 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 2.7e-57
Identity = 111/225 (49.33%), Postives = 159/225 (70.67%), Query Frame = 0

Query: 16  RVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYPTFKGQVLNGRQLWDL 75
           RVLSIQSH V+GYVGN++A FPLQ+LG++VD +NSVQFSNHTGY  +KGQVLN  +L +L
Sbjct: 6   RVLSIQSHVVRGYVGNRAATFPLQVLGFEVDAVNSVQFSNHTGYSHWKGQVLNSDELQEL 65

Query: 76  IEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTYVCDPVMGD----EGK 135
            +GL+ N +  Y ++LTGY    SFL  V+++V +L+  NP+L YVCDPVMGD    EG 
Sbjct: 66  YDGLKLNSVNQYDYVLTGYTRDKSFLAMVVDIVQELKQQNPRLVYVCDPVMGDQRDGEGA 125

Query: 136 LYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNILHAAGPSKVVI 195
           +YVP++L+ VYREKV+PVA ++TPNQFEAE LTG +I ++ +  E  ++LH+ GP  VVI
Sbjct: 126 MYVPDDLLPVYREKVVPVADIITPNQFEAELLTGRKIHTQEEALEVMDMLHSMGPDTVVI 185

Query: 196 TSINM-----NGELLLIGSHQ---KNEGQAPEQFKIMIPKIPAYF 229
           TS ++     +  L+ +GS +    +     ++ ++ + K+ A F
Sbjct: 186 TSSDLLSPRGSDYLMALGSQRTRAPDGSMVTQRIRMEMHKVDAVF 230

BLAST of CSPI03G22270 vs. ExPASy Swiss-Prot
Match: O00764 (Pyridoxal kinase OS=Homo sapiens OX=9606 GN=PDXK PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 4.6e-57
Identity = 109/225 (48.44%), Postives = 158/225 (70.22%), Query Frame = 0

Query: 16  RVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYPTFKGQVLNGRQLWDL 75
           RVLSIQSH ++GYVGN++A FPLQ+LG+++D +NSVQFSNHTGY  +KGQVLN  +L +L
Sbjct: 6   RVLSIQSHVIRGYVGNRAATFPLQVLGFEIDAVNSVQFSNHTGYAHWKGQVLNSDELQEL 65

Query: 76  IEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTYVCDPVMGD----EGK 135
            EGL  N +  Y ++LTGY    SFL  V+++V +L+  NP+L YVCDPV+GD    EG 
Sbjct: 66  YEGLRLNNMNKYDYVLTGYTRDKSFLAMVVDIVQELKQQNPRLVYVCDPVLGDKWDGEGS 125

Query: 136 LYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNILHAAGPSKVVI 195
           +YVPE+L+ VY+EKV+P+A ++TPNQFEAE L+G +I S+ +     ++LH+ GP  VVI
Sbjct: 126 MYVPEDLLPVYKEKVVPLADIITPNQFEAELLSGRKIHSQEEALRVMDMLHSMGPDTVVI 185

Query: 196 TSINM-----NGELLLIGSHQKNE---GQAPEQFKIMIPKIPAYF 229
           TS ++     +  L+++GS ++         E+ ++ I K+ A F
Sbjct: 186 TSSDLPSPQGSNYLIVLGSQRRRNPAGSVVMERIRMDIRKVDAVF 230

BLAST of CSPI03G22270 vs. ExPASy TrEMBL
Match: A0A0A0LCU0 (Pyridoxal kinase OS=Cucumis sativus OX=3659 GN=Csa_3G363190 PE=3 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 9.7e-127
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 0

Query: 1   MSPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60
           MSPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP
Sbjct: 1   MSPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60

Query: 61  TFKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY 120
           TFKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY
Sbjct: 61  TFKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY 120

Query: 121 VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI 180
           VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI
Sbjct: 121 VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI 180

Query: 181 LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT 230
           LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT
Sbjct: 181 LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT 229

BLAST of CSPI03G22270 vs. ExPASy TrEMBL
Match: A0A5D3BQN4 (Pyridoxal kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G00360 PE=3 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 1.5e-124
Identity = 226/229 (98.69%), Postives = 227/229 (99.13%), Query Frame = 0

Query: 1   MSPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60
           MSPPILSLALPS TGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP
Sbjct: 1   MSPPILSLALPSATGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60

Query: 61  TFKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY 120
           TFKGQVLNG QLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY
Sbjct: 61  TFKGQVLNGGQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY 120

Query: 121 VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI 180
           VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI
Sbjct: 121 VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI 180

Query: 181 LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT 230
           LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKI+IPKIPAYFT
Sbjct: 181 LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIVIPKIPAYFT 229

BLAST of CSPI03G22270 vs. ExPASy TrEMBL
Match: A0A1S3C498 (Pyridoxal kinase OS=Cucumis melo OX=3656 GN=LOC103496845 PE=3 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 1.5e-124
Identity = 226/229 (98.69%), Postives = 227/229 (99.13%), Query Frame = 0

Query: 1   MSPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60
           MSPPILSLALPS TGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP
Sbjct: 1   MSPPILSLALPSATGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60

Query: 61  TFKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY 120
           TFKGQVLNG QLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY
Sbjct: 61  TFKGQVLNGGQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY 120

Query: 121 VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI 180
           VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI
Sbjct: 121 VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI 180

Query: 181 LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT 230
           LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKI+IPKIPAYFT
Sbjct: 181 LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIVIPKIPAYFT 229

BLAST of CSPI03G22270 vs. ExPASy TrEMBL
Match: A0A6J1KGY9 (Pyridoxal kinase OS=Cucurbita maxima OX=3661 GN=LOC111493128 PE=3 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 1.4e-120
Identity = 217/229 (94.76%), Postives = 225/229 (98.25%), Query Frame = 0

Query: 1   MSPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60
           M+PPILSLALPS TGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP
Sbjct: 1   MTPPILSLALPSVTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60

Query: 61  TFKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY 120
           TFKGQVLNG QLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVL+VVDKLR VNPKLTY
Sbjct: 61  TFKGQVLNGGQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLKVVDKLRSVNPKLTY 120

Query: 121 VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI 180
           VCDPVMGDEGKLYVP+ELVSVYREKV+PVAS+LTPNQFEAEQLTGLRIQSEGDGREACNI
Sbjct: 121 VCDPVMGDEGKLYVPQELVSVYREKVVPVASMLTPNQFEAEQLTGLRIQSEGDGREACNI 180

Query: 181 LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT 230
           LHAAGP+KVVITSI+MNGELLLIGSHQKNEGQ+PEQFKI IPKIPAYFT
Sbjct: 181 LHAAGPTKVVITSISMNGELLLIGSHQKNEGQSPEQFKIAIPKIPAYFT 229

BLAST of CSPI03G22270 vs. ExPASy TrEMBL
Match: A0A6J1G9F5 (Pyridoxal kinase OS=Cucurbita moschata OX=3662 GN=LOC111452161 PE=3 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 3.0e-120
Identity = 216/229 (94.32%), Postives = 225/229 (98.25%), Query Frame = 0

Query: 1   MSPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60
           M+PPILSLALPS TGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP
Sbjct: 1   MTPPILSLALPSATGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60

Query: 61  TFKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY 120
           TFKGQVLNG QLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVL+VV+KLR VNPKLTY
Sbjct: 61  TFKGQVLNGGQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLKVVEKLRSVNPKLTY 120

Query: 121 VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI 180
           VCDPVMGDEGKLYVP+ELVSVYREKV+PVAS+LTPNQFEAEQLTGLRIQSEGDGREACNI
Sbjct: 121 VCDPVMGDEGKLYVPQELVSVYREKVVPVASMLTPNQFEAEQLTGLRIQSEGDGREACNI 180

Query: 181 LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT 230
           LHAAGP+KVVITSI+MNGELLLIGSHQKNEGQ+PEQFKI IPKIPAYFT
Sbjct: 181 LHAAGPTKVVITSISMNGELLLIGSHQKNEGQSPEQFKIAIPKIPAYFT 229

BLAST of CSPI03G22270 vs. NCBI nr
Match: XP_004149445.1 (pyridoxal kinase isoform X1 [Cucumis sativus] >KGN57886.1 hypothetical protein Csa_011119 [Cucumis sativus])

HSP 1 Score: 462.6 bits (1189), Expect = 2.0e-126
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 0

Query: 1   MSPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60
           MSPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP
Sbjct: 1   MSPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60

Query: 61  TFKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY 120
           TFKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY
Sbjct: 61  TFKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY 120

Query: 121 VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI 180
           VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI
Sbjct: 121 VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI 180

Query: 181 LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT 230
           LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT
Sbjct: 181 LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT 229

BLAST of CSPI03G22270 vs. NCBI nr
Match: XP_008457077.1 (PREDICTED: pyridoxal kinase [Cucumis melo] >KAA0060743.1 pyridoxal kinase [Cucumis melo var. makuwa] >TYK01484.1 pyridoxal kinase [Cucumis melo var. makuwa])

HSP 1 Score: 455.3 bits (1170), Expect = 3.2e-124
Identity = 226/229 (98.69%), Postives = 227/229 (99.13%), Query Frame = 0

Query: 1   MSPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60
           MSPPILSLALPS TGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP
Sbjct: 1   MSPPILSLALPSATGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60

Query: 61  TFKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY 120
           TFKGQVLNG QLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY
Sbjct: 61  TFKGQVLNGGQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY 120

Query: 121 VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI 180
           VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI
Sbjct: 121 VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI 180

Query: 181 LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT 230
           LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKI+IPKIPAYFT
Sbjct: 181 LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIVIPKIPAYFT 229

BLAST of CSPI03G22270 vs. NCBI nr
Match: XP_038875447.1 (pyridoxal kinase [Benincasa hispida])

HSP 1 Score: 450.7 bits (1158), Expect = 7.9e-123
Identity = 223/229 (97.38%), Postives = 226/229 (98.69%), Query Frame = 0

Query: 1   MSPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60
           MSPPILSLALPS TGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP
Sbjct: 1   MSPPILSLALPSATGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60

Query: 61  TFKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY 120
           TFKGQVLNG QLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVL+VVDKLRLVNPKLTY
Sbjct: 61  TFKGQVLNGGQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLKVVDKLRLVNPKLTY 120

Query: 121 VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI 180
           VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI
Sbjct: 121 VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI 180

Query: 181 LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT 230
           LHAAGP+KVVITSINMNGELLLIGSHQKNEGQAPEQFKI IP+IPAYFT
Sbjct: 181 LHAAGPTKVVITSINMNGELLLIGSHQKNEGQAPEQFKIAIPRIPAYFT 229

BLAST of CSPI03G22270 vs. NCBI nr
Match: XP_022998513.1 (pyridoxal kinase-like [Cucurbita maxima])

HSP 1 Score: 442.2 bits (1136), Expect = 2.8e-120
Identity = 217/229 (94.76%), Postives = 225/229 (98.25%), Query Frame = 0

Query: 1   MSPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60
           M+PPILSLALPS TGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP
Sbjct: 1   MTPPILSLALPSVTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60

Query: 61  TFKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY 120
           TFKGQVLNG QLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVL+VVDKLR VNPKLTY
Sbjct: 61  TFKGQVLNGGQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLKVVDKLRSVNPKLTY 120

Query: 121 VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI 180
           VCDPVMGDEGKLYVP+ELVSVYREKV+PVAS+LTPNQFEAEQLTGLRIQSEGDGREACNI
Sbjct: 121 VCDPVMGDEGKLYVPQELVSVYREKVVPVASMLTPNQFEAEQLTGLRIQSEGDGREACNI 180

Query: 181 LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT 230
           LHAAGP+KVVITSI+MNGELLLIGSHQKNEGQ+PEQFKI IPKIPAYFT
Sbjct: 181 LHAAGPTKVVITSISMNGELLLIGSHQKNEGQSPEQFKIAIPKIPAYFT 229

BLAST of CSPI03G22270 vs. NCBI nr
Match: KAG6607001.1 (Pyridoxal kinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 441.0 bits (1133), Expect = 6.2e-120
Identity = 216/229 (94.32%), Postives = 225/229 (98.25%), Query Frame = 0

Query: 1   MSPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60
           M+PPILSLALPS TGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP
Sbjct: 1   MTPPILSLALPSATGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYP 60

Query: 61  TFKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTY 120
           TFKGQVLNG QLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVL+VV+KLR VNPKLTY
Sbjct: 61  TFKGQVLNGGQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLKVVEKLRSVNPKLTY 120

Query: 121 VCDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNI 180
           VCDPVMGDEGKLYVP+ELVSVYREKV+PVAS+LTPNQFEAEQLTGLRIQSEGDGREACNI
Sbjct: 121 VCDPVMGDEGKLYVPQELVSVYREKVVPVASMLTPNQFEAEQLTGLRIQSEGDGREACNI 180

Query: 181 LHAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT 230
           LHAAGP+KVVITSI+MNGELLLIGSHQKNEGQ+PEQFKI IPKIPAYFT
Sbjct: 181 LHAAGPTKVVITSISMNGELLLIGSHQKNEGQSPEQFKIAIPKIPAYFT 229

BLAST of CSPI03G22270 vs. TAIR 10
Match: AT5G37850.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 398.3 bits (1022), Expect = 4.3e-111
Identity = 195/228 (85.53%), Postives = 212/228 (92.98%), Query Frame = 0

Query: 2   SPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYPT 61
           +PP+LSLALPS TGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYPT
Sbjct: 37  TPPVLSLALPSDTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYPT 96

Query: 62  FKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTYV 121
           FKGQVLNG+QL DLIEGLE N+LL+YTH+LTGYIGSVSFL+T+LEV++KLR VNP LTYV
Sbjct: 97  FKGQVLNGQQLCDLIEGLEANDLLFYTHVLTGYIGSVSFLDTILEVINKLRSVNPNLTYV 156

Query: 122 CDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNIL 181
           CDPVMGDEGKLYVPEELV VYREKV+P+AS+LTPNQFEAE+LTGLRI SE DGREAC IL
Sbjct: 157 CDPVMGDEGKLYVPEELVHVYREKVVPLASMLTPNQFEAEKLTGLRINSEEDGREACAIL 216

Query: 182 HAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT 230
           HAAGPSKVVITSI + G LLLIGSHQK +G  PEQFKI+I KIPAYFT
Sbjct: 217 HAAGPSKVVITSITIGGILLLIGSHQKEKGLKPEQFKILIHKIPAYFT 264

BLAST of CSPI03G22270 vs. TAIR 10
Match: AT5G37850.2 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 398.3 bits (1022), Expect = 4.3e-111
Identity = 195/228 (85.53%), Postives = 212/228 (92.98%), Query Frame = 0

Query: 2   SPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYPT 61
           +PP+LSLALPS TGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYPT
Sbjct: 3   TPPVLSLALPSDTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYPT 62

Query: 62  FKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTYV 121
           FKGQVLNG+QL DLIEGLE N+LL+YTH+LTGYIGSVSFL+T+LEV++KLR VNP LTYV
Sbjct: 63  FKGQVLNGQQLCDLIEGLEANDLLFYTHVLTGYIGSVSFLDTILEVINKLRSVNPNLTYV 122

Query: 122 CDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNIL 181
           CDPVMGDEGKLYVPEELV VYREKV+P+AS+LTPNQFEAE+LTGLRI SE DGREAC IL
Sbjct: 123 CDPVMGDEGKLYVPEELVHVYREKVVPLASMLTPNQFEAEKLTGLRINSEEDGREACAIL 182

Query: 182 HAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT 230
           HAAGPSKVVITSI + G LLLIGSHQK +G  PEQFKI+I KIPAYFT
Sbjct: 183 HAAGPSKVVITSITIGGILLLIGSHQKEKGLKPEQFKILIHKIPAYFT 230

BLAST of CSPI03G22270 vs. TAIR 10
Match: AT5G37850.3 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 338.6 bits (867), Expect = 4.1e-93
Identity = 173/228 (75.88%), Postives = 186/228 (81.58%), Query Frame = 0

Query: 2   SPPILSLALPSPTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYPT 61
           +PP+LSLALPS TGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYPT
Sbjct: 37  TPPVLSLALPSDTGRVLSIQSHTVQGYVGNKSAVFPLQLLGYDVDPINSVQFSNHTGYPT 96

Query: 62  FKGQVLNGRQLWDLIEGLEENELLYYTHLLTGYIGSVSFLNTVLEVVDKLRLVNPKLTYV 121
           FKGQVLNG+QL DLIEGLE N+LL+YTH+LT                            V
Sbjct: 97  FKGQVLNGQQLCDLIEGLEANDLLFYTHVLT----------------------------V 156

Query: 122 CDPVMGDEGKLYVPEELVSVYREKVIPVASVLTPNQFEAEQLTGLRIQSEGDGREACNIL 181
           CDPVMGDEGKLYVPEELV VYREKV+P+AS+LTPNQFEAE+LTGLRI SE DGREAC IL
Sbjct: 157 CDPVMGDEGKLYVPEELVHVYREKVVPLASMLTPNQFEAEKLTGLRINSEEDGREACAIL 216

Query: 182 HAAGPSKVVITSINMNGELLLIGSHQKNEGQAPEQFKIMIPKIPAYFT 230
           HAAGPSKVVITSI + G LLLIGSHQK +G  PEQFKI+I KIPAYFT
Sbjct: 217 HAAGPSKVVITSITIGGILLLIGSHQKEKGLKPEQFKILIHKIPAYFT 236

BLAST of CSPI03G22270 vs. TAIR 10
Match: AT1G22940.1 (thiamin biosynthesis protein, putative )

HSP 1 Score: 55.1 bits (131), Expect = 9.0e-08
Identity = 32/102 (31.37%), Postives = 53/102 (51.96%), Query Frame = 0

Query: 92  TGYIGSVSFLNTVLEVVDKLRLVNPKLTYVCDPVM-GDEGKLYVPEELVSVYREKVIPVA 151
           TG + S   +  +L+ +       P    V DPVM    G +     ++S++RE+++P+A
Sbjct: 105 TGMLPSTEIVEVLLQNLSDF----PVRALVVDPVMVSTSGHVLAGSSILSIFRERLLPIA 164

Query: 152 SVLTPNQFEAEQ-LTGLRIQSEGDGREACNILHAAGPSKVVI 192
            ++TPN  EA   L G RI++  + R A   LH  GP  V++
Sbjct: 165 DIITPNVKEASALLDGFRIETVAEMRSAAKSLHEMGPRFVLV 202

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8W1X26.1e-11085.53Pyridoxal kinase OS=Arabidopsis thaliana OX=3702 GN=PK PE=1 SV=2[more]
O465601.4e-5852.44Pyridoxal kinase OS=Sus scrofa OX=9823 GN=PDXK PE=1 SV=1[more]
P821971.6e-5757.84Pyridoxal kinase OS=Ovis aries OX=9940 GN=PDXK PE=1 SV=1[more]
Q0II592.7e-5749.33Pyridoxal kinase OS=Bos taurus OX=9913 GN=PDXK PE=2 SV=1[more]
O007644.6e-5748.44Pyridoxal kinase OS=Homo sapiens OX=9606 GN=PDXK PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LCU09.7e-127100.00Pyridoxal kinase OS=Cucumis sativus OX=3659 GN=Csa_3G363190 PE=3 SV=1[more]
A0A5D3BQN41.5e-12498.69Pyridoxal kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G003... [more]
A0A1S3C4981.5e-12498.69Pyridoxal kinase OS=Cucumis melo OX=3656 GN=LOC103496845 PE=3 SV=1[more]
A0A6J1KGY91.4e-12094.76Pyridoxal kinase OS=Cucurbita maxima OX=3661 GN=LOC111493128 PE=3 SV=1[more]
A0A6J1G9F53.0e-12094.32Pyridoxal kinase OS=Cucurbita moschata OX=3662 GN=LOC111452161 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004149445.12.0e-126100.00pyridoxal kinase isoform X1 [Cucumis sativus] >KGN57886.1 hypothetical protein C... [more]
XP_008457077.13.2e-12498.69PREDICTED: pyridoxal kinase [Cucumis melo] >KAA0060743.1 pyridoxal kinase [Cucum... [more]
XP_038875447.17.9e-12397.38pyridoxal kinase [Benincasa hispida][more]
XP_022998513.12.8e-12094.76pyridoxal kinase-like [Cucurbita maxima][more]
KAG6607001.16.2e-12094.32Pyridoxal kinase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT5G37850.14.3e-11185.53pfkB-like carbohydrate kinase family protein [more]
AT5G37850.24.3e-11185.53pfkB-like carbohydrate kinase family protein [more]
AT5G37850.34.1e-9375.88pfkB-like carbohydrate kinase family protein [more]
AT1G22940.19.0e-0831.37thiamin biosynthesis protein, putative [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013749Pyridoxamine kinase/Phosphomethylpyrimidine kinasePFAMPF08543Phos_pyr_kincoord: 92..198
e-value: 7.5E-15
score: 55.0
IPR004625Pyridoxine kinaseTIGRFAMTIGR00687TIGR00687coord: 16..198
e-value: 2.0E-71
score: 238.6
IPR004625Pyridoxine kinasePANTHERPTHR10534PYRIDOXAL KINASEcoord: 5..229
IPR004625Pyridoxine kinaseCDDcd01173pyridoxal_pyridoxamine_kinasecoord: 16..229
e-value: 1.27574E-94
score: 275.617
IPR029056Ribokinase-likeGENE3D3.40.1190.20coord: 13..229
e-value: 1.4E-85
score: 289.2
IPR029056Ribokinase-likeSUPERFAMILY53613Ribokinase-likecoord: 15..202
NoneNo IPR availablePANTHERPTHR10534:SF2PYRIDOXAL KINASEcoord: 5..229

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G22270.1CSPI03G22270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0009443 pyridoxal 5'-phosphate salvage
cellular_component GO:0005829 cytosol
molecular_function GO:0008478 pyridoxal kinase activity