CSPI03G20680 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G20680
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Transposon Ty3-I Gag-Pol polyprotein
Location	Chr3: 16655552 .. 16656229 (-)
RNA-Seq Expression	CSPI03G20680
Synteny	CSPI03G20680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCATTTTATTCCTTGCAAAAAAACTTCCGATGCTTTGAATATAGCTAACTTGTTTTTTCGAGAAATTGTTAGATTGCATGGAATTCCTAAAACTATAGTTTCGGATAGAGATGTAAAATTCTTGAGCCATTTTTGGAGATCCTTATGGAAGAAATTCGACACCAACCTCCTGTTTTGGACCGCCAGTCACCCACAAACTGACGGCCAAACAGAGGTGACCAACCGAACTCTTAGCAACCTCATTCGATGCCTTAGCGAGGATAAGCCTAAACAATGGGACCTAGCTTTACCTCAAGCAGAATTCGCCTTCAACCACATGGCAAATCGCTCAACAGGGAAGTCTCCATTTGAGGTTGTATACACTAGTCTCCCTCGGGTAACTTTTGATTTAGCTAATCTACCTTCTGTTGTTGATGTCAGTATGGAGGCTGAAGCAATGGCTGAACGTATCTCCAAGCTACACCAAGAAGTCAAGAGTCATCTAGAGCTTGCTAATGACTCCTACAAAACAGCCGCCAACTATCACAAACGCTTTAAGGAATATCAAGTAGGTGACCTAGTTATGGTGCACCTTCGAAAGTCAAGACTATATGCTGGTCATTACTCCAAAGTGACCAACAAGCGCATGGGCCCATTTCAAATATTGGAAAGACTTGGCCCCAATGCTTACCGA

mRNA sequence

ATGGCTCATTTTATTCCTTGCAAAAAAACTTCCGATGCTTTGAATATAGCTAACTTGTTTTTTCGAGAAATTGTTAGATTGCATGGAATTCCTAAAACTATAGTTTCGGATAGAGATGTAAAATTCTTGAGCCATTTTTGGAGATCCTTATGGAAGAAATTCGACACCAACCTCCTGTTTTGGACCGCCAGTCACCCACAAACTGACGGCCAAACAGAGGTGACCAACCGAACTCTTAGCAACCTCATTCGATGCCTTAGCGAGGATAAGCCTAAACAATGGGACCTAGCTTTACCTCAAGCAGAATTCGCCTTCAACCACATGGCAAATCGCTCAACAGGGAAGTCTCCATTTGAGGTTGTATACACTAGTCTCCCTCGGGTAACTTTTGATTTAGCTAATCTACCTTCTGTTGTTGATGTCAGTATGGAGGCTGAAGCAATGGCTGAACGTATCTCCAAGCTACACCAAGAAGTCAAGAGTCATCTAGAGCTTGCTAATGACTCCTACAAAACAGCCGCCAACTATCACAAACGCTTTAAGGAATATCAAGTAGGTGACCTAGTTATGGTGCACCTTCGAAAGTCAAGACTATATGCTGGTCATTACTCCAAAGTGACCAACAAGCGCATGGGCCCATTTCAAATATTGGAAAGACTTGGCCCCAATGCTTACCGA

Coding sequence (CDS)

ATGGCTCATTTTATTCCTTGCAAAAAAACTTCCGATGCTTTGAATATAGCTAACTTGTTTTTTCGAGAAATTGTTAGATTGCATGGAATTCCTAAAACTATAGTTTCGGATAGAGATGTAAAATTCTTGAGCCATTTTTGGAGATCCTTATGGAAGAAATTCGACACCAACCTCCTGTTTTGGACCGCCAGTCACCCACAAACTGACGGCCAAACAGAGGTGACCAACCGAACTCTTAGCAACCTCATTCGATGCCTTAGCGAGGATAAGCCTAAACAATGGGACCTAGCTTTACCTCAAGCAGAATTCGCCTTCAACCACATGGCAAATCGCTCAACAGGGAAGTCTCCATTTGAGGTTGTATACACTAGTCTCCCTCGGGTAACTTTTGATTTAGCTAATCTACCTTCTGTTGTTGATGTCAGTATGGAGGCTGAAGCAATGGCTGAACGTATCTCCAAGCTACACCAAGAAGTCAAGAGTCATCTAGAGCTTGCTAATGACTCCTACAAAACAGCCGCCAACTATCACAAACGCTTTAAGGAATATCAAGTAGGTGACCTAGTTATGGTGCACCTTCGAAAGTCAAGACTATATGCTGGTCATTACTCCAAAGTGACCAACAAGCGCATGGGCCCATTTCAAATATTGGAAAGACTTGGCCCCAATGCTTACCGA

Protein sequence

MAHFIPCKKTSDALNIANLFFREIVRLHGIPKTIVSDRDVKFLSHFWRSLWKKFDTNLLFWTASHPQTDGQTEVTNRTLSNLIRCLSEDKPKQWDLALPQAEFAFNHMANRSTGKSPFEVVYTSLPRVTFDLANLPSVVDVSMEAEAMAERISKLHQEVKSHLELANDSYKTAANYHKRFKEYQVGDLVMVHLRKSRLYAGHYSKVTNKRMGPFQILERLGPNAYR

Homology

[Close]

BLAST of CSPI03G20680 vs. ExPASy Swiss-Prot
Match: Q99315 (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-G PE=1 SV=3)

HSP 1 Score: 142.5 bits (358), Expect = 5.8e-33
Identity = 82/224 (36.61%), Postives = 122/224 (54.46%), Query Frame = 0


Query: 2    AHFIPCKKTSDALNIANLFFREIVRLHGIPKTIVSDRDVKFLSHFWRSLWKKFDTNLLFW 61
            AHFI  +KT DA  + +L FR I   HG P+TI SDRDV+  +  ++ L K+        
Sbjct: 1201 AHFIATRKTLDATQLIDLLFRYIFSYHGFPRTITSDRDVRMTADKYQELTKRLGIKSTMS 1260

Query: 62   TASHPQTDGQTEVTNRTLSNLIRCLSEDKPKQWDLALPQAEFAFNHMANRSTGKSPFEVV 121
            +A+HPQTDGQ+E T +TL+ L+R  +    + W + LPQ EF +N    R+ GKSPFE+ 
Sbjct: 1261 SANHPQTDGQSERTIQTLNRLLRAYASTNIQNWHVYLPQIEFVYNSTPTRTLGKSPFEID 1320

Query: 122  YTSLPRVTFDLANLPSVVDVSMEAEAMAERISKLHQEVKSHLELANDSYKTAANYHKRFK 181
               LP  T  + +   V   S  A  +A+ +  L  + K  LE A    +T  N  ++  
Sbjct: 1321 LGYLPN-TPAIKSDDEVNARSFTAVELAKHLKALTIQTKEQLEHAQIEMETNNNQRRKPL 1380

Query: 182  EYQVGDLVMVHLRKSRLYAGHYSKVTNKRMGPFQILERLGPNAY 226
               +GD V+VH R +    G Y KV    +GPF++++++  NAY
Sbjct: 1381 LLNIGDHVLVH-RDAYFKKGAYMKVQQIYVGPFRVVKKINDNAY 1422

[Close]

BLAST of CSPI03G20680 vs. ExPASy Swiss-Prot
Match: Q7LHG5 (Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-I PE=1 SV=2)

HSP 1 Score: 141.4 bits (355), Expect = 1.3e-32
Identity = 82/224 (36.61%), Postives = 121/224 (54.02%), Query Frame = 0


Query: 2    AHFIPCKKTSDALNIANLFFREIVRLHGIPKTIVSDRDVKFLSHFWRSLWKKFDTNLLFW 61
            AHFI  +KT DA  + +L FR I   HG P+TI SDRDV+  +  ++ L K+        
Sbjct: 1227 AHFIATRKTLDATQLIDLLFRYIFSYHGFPRTITSDRDVRMTADKYQELTKRLGIKSTMS 1286

Query: 62   TASHPQTDGQTEVTNRTLSNLIRCLSEDKPKQWDLALPQAEFAFNHMANRSTGKSPFEVV 121
            +A+HPQTDGQ+E T +TL+ L+R       + W + LPQ EF +N    R+ GKSPFE+ 
Sbjct: 1287 SANHPQTDGQSERTIQTLNRLLRAYVSTNIQNWHVYLPQIEFVYNSTPTRTLGKSPFEID 1346

Query: 122  YTSLPRVTFDLANLPSVVDVSMEAEAMAERISKLHQEVKSHLELANDSYKTAANYHKRFK 181
               LP  T  + +   V   S  A  +A+ +  L  + K  LE A    +T  N  ++  
Sbjct: 1347 LGYLPN-TPAIKSDDEVNARSFTAVELAKHLKALTIQTKEQLEHAQIEMETNNNQRRKPL 1406

Query: 182  EYQVGDLVMVHLRKSRLYAGHYSKVTNKRMGPFQILERLGPNAY 226
               +GD V+VH R +    G Y KV    +GPF++++++  NAY
Sbjct: 1407 LLNIGDHVLVH-RDAYFKKGAYMKVQQIYVGPFRVVKKINDNAY 1448

[Close]

BLAST of CSPI03G20680 vs. ExPASy Swiss-Prot
Match: Q9UR07 (Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-11 PE=3 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 9.0e-26
Identity = 71/226 (31.42%), Postives = 116/226 (51.33%), Query Frame = 0


Query: 1    MAHFIPCKKTSDALNIANLFFREIVRLHGIPKTIVSDRDVKFLSHFWRSLWKKFDTNLLF 60
            MA  +PC K+  A   A +F + ++   G PK I++D D  F S  W+    K++  + F
Sbjct: 1014 MAILVPCTKSITAEQTARMFDQRVIAYFGNPKEIIADNDHIFTSQTWKDFAHKYNFVMKF 1073

Query: 61   WTASHPQTDGQTEVTNRTLSNLIRCLSEDKPKQWDLALPQAEFAFNHMANRSTGKSPFEV 120
                 PQTDGQTE TN+T+  L+RC+    P  W   +   + ++N+  + +T  +PFE+
Sbjct: 1074 SLPYRPQTDGQTERTNQTVEKLLRCVCSTHPNTWVDHISLVQQSYNNAIHSATQMTPFEI 1133

Query: 121  VYTSLPRVTFDLANLPSVVDVSMEAEAMAERISKLHQEVKSHLELANDSYKTAANYH-KR 180
            V+   P ++     LPS  D   + +  ++   ++ Q VK HL   N   K   +   + 
Sbjct: 1134 VHRYSPALS--PLELPSFSD---KTDENSQETIQVFQTVKEHLNTNNIKMKKYFDMKIQE 1193

Query: 181  FKEYQVGDLVMVHLRKSRLYAGHYSKVTNKRMGPFQILERLGPNAY 226
             +E+Q GDLVMV   K+  +    +K+     GPF +L++ GPN Y
Sbjct: 1194 IEEFQPGDLVMVKRTKTG-FLHKSNKLAPSFAGPFYVLQKSGPNNY 1233

[Close]

BLAST of CSPI03G20680 vs. ExPASy Swiss-Prot
Match: P0CT41 (Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-12 PE=3 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 9.0e-26
Identity = 71/226 (31.42%), Postives = 116/226 (51.33%), Query Frame = 0


Query: 1    MAHFIPCKKTSDALNIANLFFREIVRLHGIPKTIVSDRDVKFLSHFWRSLWKKFDTNLLF 60
            MA  +PC K+  A   A +F + ++   G PK I++D D  F S  W+    K++  + F
Sbjct: 1014 MAILVPCTKSITAEQTARMFDQRVIAYFGNPKEIIADNDHIFTSQTWKDFAHKYNFVMKF 1073

Query: 61   WTASHPQTDGQTEVTNRTLSNLIRCLSEDKPKQWDLALPQAEFAFNHMANRSTGKSPFEV 120
                 PQTDGQTE TN+T+  L+RC+    P  W   +   + ++N+  + +T  +PFE+
Sbjct: 1074 SLPYRPQTDGQTERTNQTVEKLLRCVCSTHPNTWVDHISLVQQSYNNAIHSATQMTPFEI 1133

Query: 121  VYTSLPRVTFDLANLPSVVDVSMEAEAMAERISKLHQEVKSHLELANDSYKTAANYH-KR 180
            V+   P ++     LPS  D   + +  ++   ++ Q VK HL   N   K   +   + 
Sbjct: 1134 VHRYSPALS--PLELPSFSD---KTDENSQETIQVFQTVKEHLNTNNIKMKKYFDMKIQE 1193

Query: 181  FKEYQVGDLVMVHLRKSRLYAGHYSKVTNKRMGPFQILERLGPNAY 226
             +E+Q GDLVMV   K+  +    +K+     GPF +L++ GPN Y
Sbjct: 1194 IEEFQPGDLVMVKRTKTG-FLHKSNKLAPSFAGPFYVLQKSGPNNY 1233

[Close]

BLAST of CSPI03G20680 vs. ExPASy Swiss-Prot
Match: P0CT34 (Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-1 PE=3 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 9.0e-26
Identity = 71/226 (31.42%), Postives = 116/226 (51.33%), Query Frame = 0


Query: 1    MAHFIPCKKTSDALNIANLFFREIVRLHGIPKTIVSDRDVKFLSHFWRSLWKKFDTNLLF 60
            MA  +PC K+  A   A +F + ++   G PK I++D D  F S  W+    K++  + F
Sbjct: 1014 MAILVPCTKSITAEQTARMFDQRVIAYFGNPKEIIADNDHIFTSQTWKDFAHKYNFVMKF 1073

Query: 61   WTASHPQTDGQTEVTNRTLSNLIRCLSEDKPKQWDLALPQAEFAFNHMANRSTGKSPFEV 120
                 PQTDGQTE TN+T+  L+RC+    P  W   +   + ++N+  + +T  +PFE+
Sbjct: 1074 SLPYRPQTDGQTERTNQTVEKLLRCVCSTHPNTWVDHISLVQQSYNNAIHSATQMTPFEI 1133

Query: 121  VYTSLPRVTFDLANLPSVVDVSMEAEAMAERISKLHQEVKSHLELANDSYKTAANYH-KR 180
            V+   P ++     LPS  D   + +  ++   ++ Q VK HL   N   K   +   + 
Sbjct: 1134 VHRYSPALS--PLELPSFSD---KTDENSQETIQVFQTVKEHLNTNNIKMKKYFDMKIQE 1193

Query: 181  FKEYQVGDLVMVHLRKSRLYAGHYSKVTNKRMGPFQILERLGPNAY 226
             +E+Q GDLVMV   K+  +    +K+     GPF +L++ GPN Y
Sbjct: 1194 IEEFQPGDLVMVKRTKTG-FLHKSNKLAPSFAGPFYVLQKSGPNNY 1233

[Close]

BLAST of CSPI03G20680 vs. ExPASy TrEMBL
Match: A0A5A7UHK1 (DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold437G00380 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 2.1e-78
Identity = 144/226 (63.72%), Postives = 178/226 (78.76%), Query Frame = 0


Query: 1   MAHFIPCKKTSDALNIANLFFREIVRLHGIPKTIVSDRDVKFLSHFWRSLWKKFDTNLLF 60
           M+HFI CKKT+DA+ IANLFFRE+VR+H +PK+IVSDRDVKFLSHFWR+LWKKFDT L +
Sbjct: 296 MSHFIACKKTNDAIYIANLFFREVVRIHRVPKSIVSDRDVKFLSHFWRTLWKKFDTTLNY 355

Query: 61  WTASHPQTDGQTEVTNRTLSNLIRCLSEDKPKQWDLALPQAEFAFNHMANRSTGKSPFEV 120
            T +HPQTDGQTEVTNRTL NLIRCLS  KPKQWDLAL QAEFAFN+M NRSTG+ PF++
Sbjct: 356 NTIAHPQTDGQTEVTNRTLGNLIRCLSGTKPKQWDLALAQAEFAFNNMKNRSTGECPFKI 415

Query: 121 VYTSLPRVTFDLANLPSVVDVSMEAEAMAERISKLHQEVKSHLELANDSYKTAANYHKRF 180
           VYT  PR+T DL +LP+ V++  EAE M   I KLH+EV  HL     SYK +A+  +R 
Sbjct: 416 VYTKQPRLTLDLTSLPTTVNLDKEAERMIGDIEKLHKEVYDHLVQTTYSYKKSADKKRRQ 475

Query: 181 KEYQVGDLVMVHLRKSRLYAGHYSKVTNKRMGPFQILERLGPNAYR 227
            E+  GDLVM+HLRK+R   G Y+K+ +K++ PF ILE+ G NA++
Sbjct: 476 AEFSKGDLVMIHLRKNRFPTGTYNKLKDKQIRPFPILEKYGDNAFK 521

[Close]

BLAST of CSPI03G20680 vs. ExPASy TrEMBL
Match: A0A5B7BER3 (Uncharacterized protein OS=Davidia involucrata OX=16924 GN=Din_036800 PE=4 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 5.2e-77
Identity = 140/225 (62.22%), Postives = 175/225 (77.78%), Query Frame = 0


Query: 1    MAHFIPCKKTSDALNIANLFFREIVRLHGIPKTIVSDRDVKFLSHFWRSLWKKFDTNLLF 60
            MAHFIPCKKTSDA ++ANLFFREIVRLHG+PK+I SDRDVKFLSHFWR+LW+KFDT+L +
Sbjct: 1228 MAHFIPCKKTSDASHVANLFFREIVRLHGVPKSITSDRDVKFLSHFWRTLWRKFDTSLQY 1287

Query: 61   WTASHPQTDGQTEVTNRTLSNLIRCLSEDKPKQWDLALPQAEFAFNHMANRSTGKSPFEV 120
             + +HPQTDGQTEVTNRTL NLIRC S D+PKQWD+ LPQ EFA+N M NRST K+PFE+
Sbjct: 1288 SSTAHPQTDGQTEVTNRTLGNLIRCTSGDRPKQWDVGLPQMEFAYNCMTNRSTKKTPFEI 1347

Query: 121  VYTSLPRVTFDLANLPSVVDVSMEAEAMAERISKLHQEVKSHLELANDSYKTAANYHKRF 180
            VYT  P+   DLA LP +   S+ AE  A+R   + +EVK +LE AN+ YK AA+ H+R 
Sbjct: 1348 VYTKPPKQALDLAPLPKLPGSSIAAENFADRYYTIQEEVKQNLEKANNLYKAAADKHRRP 1407

Query: 181  KEYQVGDLVMVHLRKSRLYAGHYSKVTNKRMGPFQILERLGPNAY 226
            K +  GDLVMV LRK+R   G Y+K+ N++ GPF++  ++  NAY
Sbjct: 1408 KVFTEGDLVMVFLRKNRFPVGTYNKLKNRKYGPFRVKRKINDNAY 1452

[Close]

BLAST of CSPI03G20680 vs. ExPASy TrEMBL
Match: A0A6N2LVR1 (Uncharacterized protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS287486 PE=4 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 5.8e-76
Identity = 135/226 (59.73%), Postives = 178/226 (78.76%), Query Frame = 0


Query: 1    MAHFIPCKKTSDALNIANLFFREIVRLHGIPKTIVSDRDVKFLSHFWRSLWKKFDTNLLF 60
            MAHFI CKKTSDA+++ANLFF+E+VRLHG+PK+I SDRD KFLSHFWR+LW++FDT L F
Sbjct: 1227 MAHFIACKKTSDAVHVANLFFKEVVRLHGVPKSITSDRDTKFLSHFWRTLWRRFDTTLNF 1286

Query: 61   WTASHPQTDGQTEVTNRTLSNLIRCLSEDKPKQWDLALPQAEFAFNHMANRSTGKSPFEV 120
             + SHPQTDGQTEV NRTL NLIRCLS ++PKQWDL L QAEFA+N M NRSTGK+PF+V
Sbjct: 1287 SSTSHPQTDGQTEVVNRTLGNLIRCLSGERPKQWDLTLAQAEFAYNSMLNRSTGKTPFQV 1346

Query: 121  VYTSLPRVTFDLANLPSVVDVSMEAEAMAERISKLHQEVKSHLELANDSYKTAANYHKRF 180
            VY   P+   DL  LP +  +++ AE MA+R+  + +EV+ +LE +N+ YK AA+  +R 
Sbjct: 1347 VYCQPPKHALDLVPLPKLPGMNIAAEHMADRVRAIQEEVRKNLEASNEKYKAAADKKRRL 1406

Query: 181  KEYQVGDLVMVHLRKSRLYAGHYSKVTNKRMGPFQILERLGPNAYR 227
            K ++ GDLVMV+LRK R+  G   K+++K+ GP+QIL+++  NAYR
Sbjct: 1407 KLFKEGDLVMVYLRKGRVPGGTLHKLSDKKHGPYQILQKINDNAYR 1452

[Close]

BLAST of CSPI03G20680 vs. ExPASy TrEMBL
Match: A0A5A7SSR7 (Serine/threonine-protein kinase TIO-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold261G00420 PE=4 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 8.6e-72
Identity = 133/226 (58.85%), Postives = 170/226 (75.22%), Query Frame = 0


Query: 1   MAHFIPCKKTSDALNIANLFFREIVRLHGIPKTIVSDRDVKFLSHFWRSLWKKFDTNLLF 60
           M HFI C+KT+D +  ANLFF EI+RLHG+ KTI+SD+DVKFLSHFWR+LWKK +T L +
Sbjct: 1   MTHFIACRKTNDVIYTANLFFIEIIRLHGVSKTILSDQDVKFLSHFWRTLWKKLETTLKY 60

Query: 61  WTASHPQTDGQTEVTNRTLSNLIRCLSEDKPKQWDLALPQAEFAFNHMANRSTGKSPFEV 120
            T +HPQTDGQTEVTNRTL NLIRCLS  K KQ DLAL Q +F FN+M NRSTGK PFEV
Sbjct: 61  STTAHPQTDGQTEVTNRTLRNLIRCLSGTKLKQGDLALAQVKFTFNNMKNRSTGKCPFEV 120

Query: 121 VYTSLPRVTFDLANLPSVVDVSMEAEAMAERISKLHQEVKSHLELANDSYKTAANYHKRF 180
           VY   PR+TFDLA+LP+ +D + E E M E+I  LH+EV  HL    +SYK  A+  +R 
Sbjct: 121 VYHKQPRLTFDLASLPTTMDTNTEVEKMIEKIENLHKEVHDHLMETTESYKRIADKKRRQ 180

Query: 181 KEYQVGDLVMVHLRKSRLYAGHYSKVTNKRMGPFQILERLGPNAYR 227
             +  GDLV++HL+K+R  AG Y+K+ +K++GPF+ LE+   NA++
Sbjct: 181 ATFSEGDLVIIHLQKNRFLAGTYNKLKDKQLGPFRPLEKFEDNAFK 226

[Close]

BLAST of CSPI03G20680 vs. ExPASy TrEMBL
Match: A0A5D3DT79 (Serine/threonine-protein kinase TIO-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold260G00110 PE=4 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 8.6e-72
Identity = 133/226 (58.85%), Postives = 170/226 (75.22%), Query Frame = 0


Query: 1   MAHFIPCKKTSDALNIANLFFREIVRLHGIPKTIVSDRDVKFLSHFWRSLWKKFDTNLLF 60
           M HFI C+KT+D +  ANLFF EI+RLHG+ KTI+SD+DVKFLSHFWR+LWKK +T L +
Sbjct: 1   MTHFIACRKTNDVIYTANLFFIEIIRLHGVSKTILSDQDVKFLSHFWRTLWKKLETTLKY 60

Query: 61  WTASHPQTDGQTEVTNRTLSNLIRCLSEDKPKQWDLALPQAEFAFNHMANRSTGKSPFEV 120
            T +HPQTDGQTEVTNRTL NLIRCLS  K KQ DLAL Q +F FN+M NRSTGK PFEV
Sbjct: 61  STTAHPQTDGQTEVTNRTLRNLIRCLSGTKLKQGDLALAQVKFTFNNMKNRSTGKCPFEV 120

Query: 121 VYTSLPRVTFDLANLPSVVDVSMEAEAMAERISKLHQEVKSHLELANDSYKTAANYHKRF 180
           VY   PR+TFDLA+LP+ +D + E E M E+I  LH+EV  HL    +SYK  A+  +R 
Sbjct: 121 VYHKQPRLTFDLASLPTTMDTNTEVEKMIEKIENLHKEVHDHLMETTESYKRIADKKRRQ 180

Query: 181 KEYQVGDLVMVHLRKSRLYAGHYSKVTNKRMGPFQILERLGPNAYR 227
             +  GDLV++HL+K+R  AG Y+K+ +K++GPF+ LE+   NA++
Sbjct: 181 ATFSEGDLVIIHLQKNRFLAGTYNKLKDKQLGPFRPLEKFEDNAFK 226

[Close]

BLAST of CSPI03G20680 vs. NCBI nr
Match: KAA0054760.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 302.0 bits (772), Expect = 4.4e-78
Identity = 144/226 (63.72%), Postives = 178/226 (78.76%), Query Frame = 0


Query: 1   MAHFIPCKKTSDALNIANLFFREIVRLHGIPKTIVSDRDVKFLSHFWRSLWKKFDTNLLF 60
           M+HFI CKKT+DA+ IANLFFRE+VR+H +PK+IVSDRDVKFLSHFWR+LWKKFDT L +
Sbjct: 296 MSHFIACKKTNDAIYIANLFFREVVRIHRVPKSIVSDRDVKFLSHFWRTLWKKFDTTLNY 355

Query: 61  WTASHPQTDGQTEVTNRTLSNLIRCLSEDKPKQWDLALPQAEFAFNHMANRSTGKSPFEV 120
            T +HPQTDGQTEVTNRTL NLIRCLS  KPKQWDLAL QAEFAFN+M NRSTG+ PF++
Sbjct: 356 NTIAHPQTDGQTEVTNRTLGNLIRCLSGTKPKQWDLALAQAEFAFNNMKNRSTGECPFKI 415

Query: 121 VYTSLPRVTFDLANLPSVVDVSMEAEAMAERISKLHQEVKSHLELANDSYKTAANYHKRF 180
           VYT  PR+T DL +LP+ V++  EAE M   I KLH+EV  HL     SYK +A+  +R 
Sbjct: 416 VYTKQPRLTLDLTSLPTTVNLDKEAERMIGDIEKLHKEVYDHLVQTTYSYKKSADKKRRQ 475

Query: 181 KEYQVGDLVMVHLRKSRLYAGHYSKVTNKRMGPFQILERLGPNAYR 227
            E+  GDLVM+HLRK+R   G Y+K+ +K++ PF ILE+ G NA++
Sbjct: 476 AEFSKGDLVMIHLRKNRFPTGTYNKLKDKQIRPFPILEKYGDNAFK 521

[Close]

BLAST of CSPI03G20680 vs. NCBI nr
Match: XP_011015163.1 (PREDICTED: uncharacterized protein LOC105118819 [Populus euphratica])

HSP 1 Score: 280.4 bits (716), Expect = 1.4e-71
Identity = 132/217 (60.83%), Postives = 172/217 (79.26%), Query Frame = 0


Query: 1    MAHFIPCKKTSDALNIANLFFREIVRLHGIPKTIVSDRDVKFLSHFWRSLWKKFDTNLLF 60
            MAHFI CKKT DA+++ANLFF+EI+RLHG+PK+I SDRD KFLSHFWR+LW++FDT+L F
Sbjct: 810  MAHFIACKKTFDAVHVANLFFKEIIRLHGVPKSITSDRDTKFLSHFWRTLWRQFDTSLNF 869

Query: 61   WTASHPQTDGQTEVTNRTLSNLIRCLSEDKPKQWDLALPQAEFAFNHMANRSTGKSPFEV 120
             + SHPQ+DGQTE+ NRTL NLIRCL  +KPKQWDL L QA+FA+N M NRS+GK PF++
Sbjct: 870  SSTSHPQSDGQTEMVNRTLGNLIRCLYGEKPKQWDLTLAQAKFAYNSMINRSSGKPPFQI 929

Query: 121  VYTSLPRVTFDLANLPSVVDVSMEAEAMAERISKLHQEVKSHLELANDSYKTAANYHKRF 180
            VY   P+   DLA LP +  +S+ AE MA+RI  +  EV+++LE +N  YK AA+  +R 
Sbjct: 930  VYCQPPQHALDLAPLPKLPGMSIAAEHMADRIKAIQGEVRTNLEKSNARYKAAADRKRRA 989

Query: 181  KEYQVGDLVMVHLRKSRLYAGHYSKVTNKRMGPFQIL 218
            K++QVGDLVMV+LRK R+ A  Y+K+ +K+ GPFQIL
Sbjct: 990  KDFQVGDLVMVYLRKGRVPARTYNKLNDKKHGPFQIL 1026

[Close]

BLAST of CSPI03G20680 vs. NCBI nr
Match: TYK26823.1 (serine/threonine-protein kinase TIO-like [Cucumis melo var. makuwa])

HSP 1 Score: 280.0 bits (715), Expect = 1.8e-71
Identity = 133/226 (58.85%), Postives = 170/226 (75.22%), Query Frame = 0


Query: 1   MAHFIPCKKTSDALNIANLFFREIVRLHGIPKTIVSDRDVKFLSHFWRSLWKKFDTNLLF 60
           M HFI C+KT+D +  ANLFF EI+RLHG+ KTI+SD+DVKFLSHFWR+LWKK +T L +
Sbjct: 1   MTHFIACRKTNDVIYTANLFFIEIIRLHGVSKTILSDQDVKFLSHFWRTLWKKLETTLKY 60

Query: 61  WTASHPQTDGQTEVTNRTLSNLIRCLSEDKPKQWDLALPQAEFAFNHMANRSTGKSPFEV 120
            T +HPQTDGQTEVTNRTL NLIRCLS  K KQ DLAL Q +F FN+M NRSTGK PFEV
Sbjct: 61  STTAHPQTDGQTEVTNRTLRNLIRCLSGTKLKQGDLALAQVKFTFNNMKNRSTGKCPFEV 120

Query: 121 VYTSLPRVTFDLANLPSVVDVSMEAEAMAERISKLHQEVKSHLELANDSYKTAANYHKRF 180
           VY   PR+TFDLA+LP+ +D + E E M E+I  LH+EV  HL    +SYK  A+  +R 
Sbjct: 121 VYHKQPRLTFDLASLPTTMDTNTEVEKMIEKIENLHKEVHDHLMETTESYKRIADKKRRQ 180

Query: 181 KEYQVGDLVMVHLRKSRLYAGHYSKVTNKRMGPFQILERLGPNAYR 227
             +  GDLV++HL+K+R  AG Y+K+ +K++GPF+ LE+   NA++
Sbjct: 181 ATFSEGDLVIIHLQKNRFLAGTYNKLKDKQLGPFRPLEKFEDNAFK 226

[Close]

BLAST of CSPI03G20680 vs. NCBI nr
Match: KAA0033503.1 (serine/threonine-protein kinase TIO-like [Cucumis melo var. makuwa])

HSP 1 Score: 280.0 bits (715), Expect = 1.8e-71
Identity = 133/226 (58.85%), Postives = 170/226 (75.22%), Query Frame = 0


Query: 1   MAHFIPCKKTSDALNIANLFFREIVRLHGIPKTIVSDRDVKFLSHFWRSLWKKFDTNLLF 60
           M HFI C+KT+D +  ANLFF EI+RLHG+ KTI+SD+DVKFLSHFWR+LWKK +T L +
Sbjct: 1   MTHFIACRKTNDVIYTANLFFIEIIRLHGVSKTILSDQDVKFLSHFWRTLWKKLETTLKY 60

Query: 61  WTASHPQTDGQTEVTNRTLSNLIRCLSEDKPKQWDLALPQAEFAFNHMANRSTGKSPFEV 120
            T +HPQTDGQTEVTNRTL NLIRCLS  K KQ DLAL Q +F FN+M NRSTGK PFEV
Sbjct: 61  STTAHPQTDGQTEVTNRTLRNLIRCLSGTKLKQGDLALAQVKFTFNNMKNRSTGKCPFEV 120

Query: 121 VYTSLPRVTFDLANLPSVVDVSMEAEAMAERISKLHQEVKSHLELANDSYKTAANYHKRF 180
           VY   PR+TFDLA+LP+ +D + E E M E+I  LH+EV  HL    +SYK  A+  +R 
Sbjct: 121 VYHKQPRLTFDLASLPTTMDTNTEVEKMIEKIENLHKEVHDHLMETTESYKRIADKKRRQ 180

Query: 181 KEYQVGDLVMVHLRKSRLYAGHYSKVTNKRMGPFQILERLGPNAYR 227
             +  GDLV++HL+K+R  AG Y+K+ +K++GPF+ LE+   NA++
Sbjct: 181 ATFSEGDLVIIHLQKNRFLAGTYNKLKDKQLGPFRPLEKFEDNAFK 226

[Close]

BLAST of CSPI03G20680 vs. NCBI nr
Match: GEX49109.1 (ATP-dependent DNA helicase RecG [Tanacetum cinerariifolium])

HSP 1 Score: 273.9 bits (699), Expect = 1.3e-69
Identity = 126/225 (56.00%), Postives = 165/225 (73.33%), Query Frame = 0


Query: 1   MAHFIPCKKTSDALNIANLFFREIVRLHGIPKTIVSDRDVKFLSHFWRSLWKKFDTNLLF 60
           MAHF+PCKKTSDA +IA  FF E+V+LHG+PKTI SDRD KFLSHFWR+LW+ F+T+L +
Sbjct: 1   MAHFLPCKKTSDASHIAGFFFWEVVQLHGVPKTITSDRDTKFLSHFWRTLWRLFETSLQY 60

Query: 61  WTASHPQTDGQTEVTNRTLSNLIRCLSEDKPKQWDLALPQAEFAFNHMANRSTGKSPFEV 120
            ++ HPQTDGQTEV N+T+ N+IRC+  D+PKQWD+ L  AEFA+N+M NRSTGKSPF V
Sbjct: 61  SSSFHPQTDGQTEVVNKTMVNIIRCMCSDRPKQWDICLAPAEFAYNNMVNRSTGKSPFSV 120

Query: 121 VYTSLPRVTFDLANLPSVVDVSMEAEAMAERISKLHQEVKSHLELANDSYKTAANYHKRF 180
           VY   P  T +L  LP ++  S+ AE  AE+I  +  +V+  LE +N  YK   + H+R 
Sbjct: 121 VYQKPPNHTLNLVPLPKILGCSIAAENFAEKIEAIQADVRLKLEASNSKYKEDMDRHRRT 180

Query: 181 KEYQVGDLVMVHLRKSRLYAGHYSKVTNKRMGPFQILERLGPNAY 226
           K Y  GDLVMVHLRK R   G Y+K+  K++GP +IL+++  NAY
Sbjct: 181 KIYAEGDLVMVHLRKERFRVGTYNKLKRKKIGPCRILKKINDNAY 225

The following BLAST results are available for this feature:

BLAST of CSPI03G20680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q99315	5.8e-33	36.61	Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]
Q7LHG5	1.3e-32	36.61	Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]
Q9UR07	9.0e-26	31.42	Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24...	[more]
P0CT41	9.0e-26	31.42	Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24...	[more]
P0CT34	9.0e-26	31.42	Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248...	[more]

BLAST of CSPI03G20680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UHK1	2.1e-78	63.72	DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A5B7BER3	5.2e-77	62.22	Uncharacterized protein OS=Davidia involucrata OX=16924 GN=Din_036800 PE=4 SV=1	[more]
A0A6N2LVR1	5.8e-76	59.73	Uncharacterized protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS287486 PE=4 SV=...	[more]
A0A5A7SSR7	8.6e-72	58.85	Serine/threonine-protein kinase TIO-like OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A5D3DT79	8.6e-72	58.85	Serine/threonine-protein kinase TIO-like OS=Cucumis melo var. makuwa OX=1194695 ...	[more]

BLAST of CSPI03G20680 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0054760.1	4.4e-78	63.72	DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa]	[more]
XP_011015163.1	1.4e-71	60.83	PREDICTED: uncharacterized protein LOC105118819 [Populus euphratica]	[more]
TYK26823.1	1.8e-71	58.85	serine/threonine-protein kinase TIO-like [Cucumis melo var. makuwa]	[more]
KAA0033503.1	1.8e-71	58.85	serine/threonine-protein kinase TIO-like [Cucumis melo var. makuwa]	[more]
GEX49109.1	1.3e-69	56.00	ATP-dependent DNA helicase RecG [Tanacetum cinerariifolium]	[more]

BLAST of CSPI03G20680 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 1..170 e-value: 7.3E-36 score: 125.3
None	No IPR available	PANTHER	PTHR24559:SF367	SUBFAMILY NOT NAMED	coord: 13..222
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 13..222
IPR001584	Integrase, catalytic core	PROSITE	PS50994	INTEGRASE	coord: 1..125 score: 13.960521
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 2..123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G20680.1	CSPI03G20680.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0016772	transferase activity, transferring phosphorus-containing groups