CSPI03G18920 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G18920
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description6-phosphogluconate dehydrogenase family protein
LocationChr3: 14543068 .. 14544396 (-)
RNA-Seq ExpressionCSPI03G18920
SyntenyCSPI03G18920
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATATGGCCTGTTTTCAAAGTATTCTTTCTTTTACTCCTTTTCTAAAAGAAAACGGCCAAAGTGATAGACGAAGGAGACTAAACAAACCAACAACTTTTGAGATCCATTCCTATTTCTACTCAGTTGCTTGAGCTTCATTATTCCTTAATCCACCATGCAAACTCCTTACCCCAACCCCATTTCTCCCACCAAAACTCGTATTGGATGGATTGGCATCGGAGTTATGGGCGCCGCTATGGCCACTCGTCTCCTCTCCGCAGGTTACTCTCTCTCTATCTACGCTCGTACCCCTTCCAAAGCTCTTGATTTACAATCCAAAGGGGCTCTTCTTGTTAACTCTGTTTTACAATTAGCTCAGACCAGCGATATTGTTTTCACAATGGTGGGTCACCCATCAGATGTTCGACAAAATGTCCTAGACGAGAATGGGATCCTACAAGGCTTGAATCCTGGTGGCGTCATCGTTGACACCACTAGTAGCCACCCCGCACTTGCCCGCGACATATTTATCGCTGCTCGTGCAAAAGGTCGTGTTAAAATTTATTCCACTTAATTTACTAGAGTTTGATGGCATGACTGAAAGCTTAGCCGGATGAGTTACTCTATGCTATGTTTAATCATTTAGCGATATTCTTGGCAGGTTGTTGGGCTGTGGACGCACCGGTGTCAGGGGGTGACATTGGGGCTAGAGATGGAAAGTTAGCCATTTTTGGTGGTGGAGATGAAGGGGTAGTGAATTGGTTATTACCTTTGTTTCAAGTGTTGGGAAAAGTAACATATGTAGGTGAAGCTGGGTGTGGGCAGAGTTGCAAGATAGCAAACCAGTTTGCAATTTCGGCGAATTTGTTGGGGCTGAGTGAAGGGTTGGTGTTTGCGGAGCGAGCGGGGTTGGACATGAAGAAGTTTGTGGAAGCTGTGAGGGGAGGGGGAGCATGGTCAATGGCAATGGAACTGTTTGCTGAGAGGATGATAGGAAGGGACTTTAGGCCTGGTGGGTTTGCTGAGTATATGGTGAAGGATTTAGGAATGGGTGTGGATGTTGTGGAAGAAGGGGATGATGAGAGGGTTGTAGTGTTGCCAGGAGCTGCATTGACCAAGCAATTGTTCTCTGCAATGGTGGCGAATGGAGATGGAAAATTTGGCAATCAAGGGTTAATCTCTGTTATTGAGAGGCTTAATGGCAAGTGAAGTGAAGATTTGAGGGTTTAATCTCACTTTAATGTTGACGGTTTTAATACCAAGCTTAAATCATAGTTTGTTTTGTGGATTTGGCACTGAAAGAGGCTTAGGGTTGAAAGACAATGTCTCTGTACTTTTATATTTGTAAC

mRNA sequence

CATATGGCCTGTTTTCAAAGTATTCTTTCTTTTACTCCTTTTCTAAAAGAAAACGGCCAAAGTGATAGACGAAGGAGACTAAACAAACCAACAACTTTTGAGATCCATTCCTATTTCTACTCAGTTGCTTGAGCTTCATTATTCCTTAATCCACCATGCAAACTCCTTACCCCAACCCCATTTCTCCCACCAAAACTCGTATTGGATGGATTGGCATCGGAGTTATGGGCGCCGCTATGGCCACTCGTCTCCTCTCCGCAGGTTACTCTCTCTCTATCTACGCTCGTACCCCTTCCAAAGCTCTTGATTTACAATCCAAAGGGGCTCTTCTTGTTAACTCTGTTTTACAATTAGCTCAGACCAGCGATATTGTTTTCACAATGGTGGGTCACCCATCAGATGTTCGACAAAATGTCCTAGACGAGAATGGGATCCTACAAGGCTTGAATCCTGGTGGCGTCATCGTTGACACCACTAGTAGCCACCCCGCACTTGCCCGCGACATATTTATCGCTGCTCGTGCAAAAGGTTGTTGGGCTGTGGACGCACCGGTGTCAGGGGGTGACATTGGGGCTAGAGATGGAAAGTTAGCCATTTTTGGTGGTGGAGATGAAGGGGTAGTGAATTGGTTATTACCTTTGTTTCAAGTGTTGGGAAAAGTAACATATGTAGGTGAAGCTGGGTGTGGGCAGAGTTGCAAGATAGCAAACCAGTTTGCAATTTCGGCGAATTTGTTGGGGCTGAGTGAAGGGTTGGTGTTTGCGGAGCGAGCGGGGTTGGACATGAAGAAGTTTGTGGAAGCTGTGAGGGGAGGGGGAGCATGGTCAATGGCAATGGAACTGTTTGCTGAGAGGATGATAGGAAGGGACTTTAGGCCTGGTGGGTTTGCTGAGTATATGGTGAAGGATTTAGGAATGGGTGTGGATGTTGTGGAAGAAGGGGATGATGAGAGGGTTGTAGTGTTGCCAGGAGCTGCATTGACCAAGCAATTGTTCTCTGCAATGGTGGCGAATGGAGATGGAAAATTTGGCAATCAAGGGTTAATCTCTGTTATTGAGAGGCTTAATGGCAAGTGAAGTGAAGATTTGAGGGTTTAATCTCACTTTAATGTTGACGGTTTTAATACCAAGCTTAAATCATAGTTTGTTTTGTGGATTTGGCACTGAAAGAGGCTTAGGGTTGAAAGACAATGTCTCTGTACTTTTATATTTGTAAC

Coding sequence (CDS)

ATGCAAACTCCTTACCCCAACCCCATTTCTCCCACCAAAACTCGTATTGGATGGATTGGCATCGGAGTTATGGGCGCCGCTATGGCCACTCGTCTCCTCTCCGCAGGTTACTCTCTCTCTATCTACGCTCGTACCCCTTCCAAAGCTCTTGATTTACAATCCAAAGGGGCTCTTCTTGTTAACTCTGTTTTACAATTAGCTCAGACCAGCGATATTGTTTTCACAATGGTGGGTCACCCATCAGATGTTCGACAAAATGTCCTAGACGAGAATGGGATCCTACAAGGCTTGAATCCTGGTGGCGTCATCGTTGACACCACTAGTAGCCACCCCGCACTTGCCCGCGACATATTTATCGCTGCTCGTGCAAAAGGTTGTTGGGCTGTGGACGCACCGGTGTCAGGGGGTGACATTGGGGCTAGAGATGGAAAGTTAGCCATTTTTGGTGGTGGAGATGAAGGGGTAGTGAATTGGTTATTACCTTTGTTTCAAGTGTTGGGAAAAGTAACATATGTAGGTGAAGCTGGGTGTGGGCAGAGTTGCAAGATAGCAAACCAGTTTGCAATTTCGGCGAATTTGTTGGGGCTGAGTGAAGGGTTGGTGTTTGCGGAGCGAGCGGGGTTGGACATGAAGAAGTTTGTGGAAGCTGTGAGGGGAGGGGGAGCATGGTCAATGGCAATGGAACTGTTTGCTGAGAGGATGATAGGAAGGGACTTTAGGCCTGGTGGGTTTGCTGAGTATATGGTGAAGGATTTAGGAATGGGTGTGGATGTTGTGGAAGAAGGGGATGATGAGAGGGTTGTAGTGTTGCCAGGAGCTGCATTGACCAAGCAATTGTTCTCTGCAATGGTGGCGAATGGAGATGGAAAATTTGGCAATCAAGGGTTAATCTCTGTTATTGAGAGGCTTAATGGCAAGTGA

Protein sequence

MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLVNSVLQLAQTSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIAARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQSCKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFRPGGFAEYMVKDLGMGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVIERLNGK*
Homology
BLAST of CSPI03G18920 vs. ExPASy Swiss-Prot
Match: Q949M8 (Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71180 PE=2 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 7.5e-100
Identity = 180/305 (59.02%), Postives = 229/305 (75.08%), Query Frame = 0

Query: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60
           M+TPYP  I P+KTRIGWIGIG+MG+AM + +++AGYS+++YAR   K  DLQ+KGA + 
Sbjct: 22  METPYPKLIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIA 81

Query: 61  NSVLQLAQTSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120
           NS  +LA+ SD+VFT+VG+ +DVR  +L ++G+L GL PGGV VD TSS P LAR+I   
Sbjct: 82  NSPKELAEMSDVVFTIVGNFNDVRSLLLGDDGVLSGLTPGGVTVDMTSSKPGLAREIHAE 141

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180
           AR + CWAVDAPVSGGD GAR+G L IF GGD  +V WL P+ + +G VTY+GEAG GQS
Sbjct: 142 ARRRNCWAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQS 201

Query: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240
           CKI NQ A ++NL+GL+EG+VFAE+AGLD  K++EAV+ G A S  M LF E ++ RD+R
Sbjct: 202 CKIGNQIAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYR 261

Query: 241 PGGFAEYMVKDLGMGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVI 300
             GFAEYMVKDLGM  +            +PGAAL+KQLF+ MVANGDGK G QG++SVI
Sbjct: 262 ATGFAEYMVKDLGMAAE----------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSVI 316

Query: 301 ERLNG 306
            RLNG
Sbjct: 322 RRLNG 316

BLAST of CSPI03G18920 vs. ExPASy Swiss-Prot
Match: Q9C991 (Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71170 PE=2 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.0e-96
Identity = 173/305 (56.72%), Postives = 221/305 (72.46%), Query Frame = 0

Query: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60
           M+T YP  I P+KTRIGWIGIG+MG+AM + +L+AGYS+++YAR   K  DLQ+KG    
Sbjct: 1   METQYPKLIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTA 60

Query: 61  NSVLQLAQTSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120
           NS  +L + SD+VFT+VG+ +DVR  +L ++G+L GL PGGV VD TSS P LAR+I+  
Sbjct: 61  NSPKELGEMSDVVFTIVGNSNDVRSLLLGDDGVLSGLKPGGVTVDMTSSKPGLAREIYAE 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180
           AR + CWAVDAPVSGGD GAR+GKL IF GGD  +V WL P+ + +G V ++G AG GQS
Sbjct: 121 ARRRDCWAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQS 180

Query: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240
           CKI NQ  + +N++GL+EG+VFAE+AGLD  K++EAV+ G A S  M LF E M  RD++
Sbjct: 181 CKIGNQICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYK 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVI 300
             GFAEYMVKDLGM          E  + +PG AL KQLF+ MVANGDGK G QG++ VI
Sbjct: 241 ATGFAEYMVKDLGMAA--------EAAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDVI 297

Query: 301 ERLNG 306
            RLNG
Sbjct: 301 RRLNG 297

BLAST of CSPI03G18920 vs. ExPASy Swiss-Prot
Match: Q9SZE1 (Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g29120 PE=1 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 3.4e-76
Identity = 144/297 (48.48%), Postives = 212/297 (71.38%), Query Frame = 0

Query: 9   ISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLVNSVLQLAQ 68
           I+P+ T+IGWIG GVMG +M   L+ AGY+++++ RT SKA  L   GA + +S   +A+
Sbjct: 33  ITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNRTISKAQTLIDMGANVADSPNSVAE 92

Query: 69  TSDIVFTMVGHPSDVRQNVLD-ENGILQGLNPGGVIVDTTSSHPALARDIFIAARAKGCW 128
            SD+VFT+VG+PSDVR  +LD ++G L GL  GGV+VD T+S P+LA +I  AA  K C+
Sbjct: 93  QSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVLVDMTTSEPSLAEEIAKAASFKNCF 152

Query: 129 AVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQSCKIANQF 188
           ++DAPVSGGD+GA++GKL+IF GGDE  V  L PLF ++GKV ++G +G GQ  K+ANQ 
Sbjct: 153 SIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLFSLMGKVNFMGTSGKGQFAKLANQI 212

Query: 189 AISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFRPGGFAEY 248
            I++ +LGL EGL++A +AGLD+KKF+EA+  G A S +++L+ +R++ RDF PG +  +
Sbjct: 213 TIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAGSKSIDLYGDRILKRDFDPGFYVNH 272

Query: 249 MVKDLGMGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVIERLN 305
            VKDLG+ ++  +       + LPG AL +QL+ ++ A+G+G  G Q L+  +ERLN
Sbjct: 273 FVKDLGICLNECQ----RMGLALPGLALAQQLYLSLKAHGEGDLGTQALLLALERLN 325

BLAST of CSPI03G18920 vs. ExPASy Swiss-Prot
Match: O34948 (Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) OX=224308 GN=ykwC PE=3 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.1e-55
Identity = 107/285 (37.54%), Postives = 183/285 (64.21%), Query Frame = 0

Query: 13  KTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLVNSVLQLAQTSDI 72
           K  IG+IG+GVMG +MA+ +L+ G+ + +Y RT  KA  +  KGA+  ++V  L++ +D+
Sbjct: 2   KKTIGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAESILQKGAIWKDTVKDLSKEADV 61

Query: 73  VFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIAARAKGCWAVDAP 132
           + TMVG+PSDV +     NGI++    G  ++D T+S P+LA+ I  AA+ K  +A+DAP
Sbjct: 62  IITMVGYPSDVEEVYFGSNGIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEKALFALDAP 121

Query: 133 VSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGK-VTYVGEAGCGQSCKIANQFAISA 192
           VSGGDIGA++G LAI  GG++      +P+F ++G+ + Y G AG GQ  K+ NQ AI+A
Sbjct: 122 VSGGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQYQGPAGSGQHTKMCNQIAIAA 181

Query: 193 NLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFRPGGFAEYMVKD 252
            ++G++E + +A+++GL+ +  ++++  G A S ++   A RM+  +F PG + ++ +KD
Sbjct: 182 GMIGVAEAMAYAQKSGLEPENVLKSITTGAAGSWSLSNLAPRMLQGNFEPGFYVKHFIKD 241

Query: 253 LGMGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGL 297
           +G+ ++  E   +E    +PG +L K L+  + A G+   G Q +
Sbjct: 242 MGIALEEAELMGEE----MPGLSLAKSLYDKLAAQGEENSGTQSI 282

BLAST of CSPI03G18920 vs. ExPASy Swiss-Prot
Match: P77161 (2-hydroxy-3-oxopropionate reductase OS=Escherichia coli (strain K12) OX=83333 GN=glxR PE=2 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 4.5e-44
Identity = 109/288 (37.85%), Postives = 159/288 (55.21%), Query Frame = 0

Query: 15  RIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLVNSVLQLAQTSDIVF 74
           ++G+IG+G+MG  MA  L  AG+ L +    P  A +L S GA+ V +  Q+ + SDI+F
Sbjct: 2   KLGFIGLGIMGTPMAINLARAGHQLHVTTIGP-VADELLSLGAVSVETARQVTEASDIIF 61

Query: 75  TMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIAARAKGCWAVDAPVS 134
            MV     V + +  ENG  +    G  IVD +S  P   +         G   +DAPVS
Sbjct: 62  IMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKRFARQVNELGGDYLDAPVS 121

Query: 135 GGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGK-VTYVGEAGCGQSCKIANQFAISANL 194
           GG+IGAR+G L+I  GGDE V   + PLF++LGK +T VG  G GQ+CK+ANQ  ++ N+
Sbjct: 122 GGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVGGNGDGQTCKVANQIIVALNI 181

Query: 195 LGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFRPGGFAEYMVKDLG 254
             +SE L+FA +AG D  +  +A+ GG A S  +E+  ERMI R F PG       KDL 
Sbjct: 182 EAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHGERMIKRTFNPGFKIALHQKDLN 241

Query: 255 MGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVIE 302
           + +    +      + LP  A  ++LF+   ANG  +  +  L+  +E
Sbjct: 242 LAL----QSAKALALNLPNTATCQELFNTCAANGGSQLDHSALVQALE 284

BLAST of CSPI03G18920 vs. ExPASy TrEMBL
Match: A0A0A0L752 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G217150 PE=4 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.8e-168
Identity = 303/306 (99.02%), Postives = 305/306 (99.67%), Query Frame = 0

Query: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60
           MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV
Sbjct: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60

Query: 61  NSVLQLAQTSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120
           NSVL+LAQ SDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA
Sbjct: 61  NSVLELAQVSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180

Query: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240
           CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR
Sbjct: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVI 300
           PGGFAEYMVKDLGMGVDVVEEG+DERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVI
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEEGEDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVI 300

Query: 301 ERLNGK 307
           ERLNGK
Sbjct: 301 ERLNGK 306

BLAST of CSPI03G18920 vs. ExPASy TrEMBL
Match: A0A1S3C3N4 (probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103496122 PE=4 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.1e-157
Identity = 282/306 (92.16%), Postives = 297/306 (97.06%), Query Frame = 0

Query: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60
           M+TPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYS++IYAR+PSKALDLQSKGALLV
Sbjct: 1   METPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSVTIYARSPSKALDLQSKGALLV 60

Query: 61  NSVLQLAQTSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120
           NSVL+LAQTSDIVFTMVGHPSDVRQNVLD+NGILQGLNPGGVIVDTTSSHPALAR+IF A
Sbjct: 61  NSVLELAQTSDIVFTMVGHPSDVRQNVLDDNGILQGLNPGGVIVDTTSSHPALARNIFTA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLF+VLGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVGEAGCGQS 180

Query: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240
           CKIANQ  ISANL+GLSEGL+FAERAGLD KKF+EAVRGG A SMAMELF ERMIGRDFR
Sbjct: 181 CKIANQIPISANLMGLSEGLMFAERAGLDTKKFLEAVRGGAAGSMAMELFGERMIGRDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVI 300
           PGG+AEYMVKDLGM VD+VEEG+DERVVVLPGAAL KQLFSAMVANGDGKFG+QGLISVI
Sbjct: 241 PGGYAEYMVKDLGMAVDIVEEGEDERVVVLPGAALIKQLFSAMVANGDGKFGSQGLISVI 300

Query: 301 ERLNGK 307
           ERLNGK
Sbjct: 301 ERLNGK 306

BLAST of CSPI03G18920 vs. ExPASy TrEMBL
Match: A0A5A7TCQ0 (Putative 3-hydroxyisobutyrate dehydrogenase-like 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold84G001550 PE=4 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.1e-157
Identity = 281/305 (92.13%), Postives = 296/305 (97.05%), Query Frame = 0

Query: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60
           M+TPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYS++IYAR+PSKALDLQSKGALLV
Sbjct: 1   METPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSVTIYARSPSKALDLQSKGALLV 60

Query: 61  NSVLQLAQTSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120
           NSVL+LAQTSDIVFTMVGHPSDVRQNVLD+NGILQGLNPGGVIVDTTSSHPALAR+IF A
Sbjct: 61  NSVLELAQTSDIVFTMVGHPSDVRQNVLDDNGILQGLNPGGVIVDTTSSHPALARNIFTA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLF+VLGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVGEAGCGQS 180

Query: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240
           CKIANQ  ISANL+GLSEGL+FAERAGLD KKF+EAVRGG A SMAMELF ERMIGRDFR
Sbjct: 181 CKIANQIPISANLMGLSEGLMFAERAGLDTKKFLEAVRGGAAGSMAMELFGERMIGRDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVI 300
           PGG+AEYMVKDLGM VD+VEEG+DERVVVLPGAAL KQLFSAMVANGDGKFG+QGLISVI
Sbjct: 241 PGGYAEYMVKDLGMAVDIVEEGEDERVVVLPGAALIKQLFSAMVANGDGKFGSQGLISVI 300

Query: 301 ERLNG 306
           ERLNG
Sbjct: 301 ERLNG 305

BLAST of CSPI03G18920 vs. ExPASy TrEMBL
Match: A0A5D3BNN2 (Putative 3-hydroxyisobutyrate dehydrogenase-like 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold252G00250 PE=4 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.5e-156
Identity = 280/305 (91.80%), Postives = 295/305 (96.72%), Query Frame = 0

Query: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60
           M+TPYPNPISPTKTRIGWIGIGVMGAAMATRLL AGYS++IYAR+PSKALDLQSKGALLV
Sbjct: 1   METPYPNPISPTKTRIGWIGIGVMGAAMATRLLCAGYSVTIYARSPSKALDLQSKGALLV 60

Query: 61  NSVLQLAQTSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120
           NSVL+LAQTSDIVFTMVGHPSDVRQNVLD+NGILQGLNPGGVIVDTTSSHPALAR+IF A
Sbjct: 61  NSVLELAQTSDIVFTMVGHPSDVRQNVLDDNGILQGLNPGGVIVDTTSSHPALARNIFTA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLF+VLGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVGEAGCGQS 180

Query: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240
           CKIANQ  ISANL+GLSEGL+FAERAGLD KKF+EAVRGG A SMAMELF ERMIGRDFR
Sbjct: 181 CKIANQIPISANLMGLSEGLMFAERAGLDTKKFLEAVRGGAAGSMAMELFGERMIGRDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVI 300
           PGG+AEYMVKDLGM V++VEEG+DERVVVLPGAAL KQLFSAMVANGDGKFGNQGLISVI
Sbjct: 241 PGGYAEYMVKDLGMAVNIVEEGEDERVVVLPGAALIKQLFSAMVANGDGKFGNQGLISVI 300

Query: 301 ERLNG 306
           ERLNG
Sbjct: 301 ERLNG 305

BLAST of CSPI03G18920 vs. ExPASy TrEMBL
Match: A0A0A0LC06 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G214010 PE=4 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 4.1e-149
Identity = 269/276 (97.46%), Postives = 273/276 (98.91%), Query Frame = 0

Query: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60
           M+TPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV
Sbjct: 1   METPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60

Query: 61  NSVLQLAQTSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120
           NSVL+LAQ SDIVFTMVGHPSDVRQNVLDENGILQGLNPGGV+VDTTSSHPALARDIF A
Sbjct: 61  NSVLELAQVSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVVVDTTSSHPALARDIFTA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180

Query: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240
           CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR
Sbjct: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGDDERVVVLPGAALT 277
           PGGFAEYMVKDLG+GVDVVEEGDDERVVVLPGA LT
Sbjct: 241 PGGFAEYMVKDLGIGVDVVEEGDDERVVVLPGAPLT 276

BLAST of CSPI03G18920 vs. NCBI nr
Match: XP_004149969.3 (probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucumis sativus] >KAE8650526.1 hypothetical protein Csa_011735 [Cucumis sativus])

HSP 1 Score: 604.7 bits (1558), Expect = 4.3e-169
Identity = 305/306 (99.67%), Postives = 306/306 (100.00%), Query Frame = 0

Query: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60
           MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV
Sbjct: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60

Query: 61  NSVLQLAQTSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120
           NSVLQLAQTSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA
Sbjct: 61  NSVLQLAQTSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180

Query: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240
           CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR
Sbjct: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVI 300
           PGGFAEYMVKDLGMGVDVVEEG+DERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVI
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEEGEDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVI 300

Query: 301 ERLNGK 307
           ERLNGK
Sbjct: 301 ERLNGK 306

BLAST of CSPI03G18920 vs. NCBI nr
Match: XP_008456080.1 (PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucumis melo])

HSP 1 Score: 565.8 bits (1457), Expect = 2.2e-157
Identity = 282/306 (92.16%), Postives = 297/306 (97.06%), Query Frame = 0

Query: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60
           M+TPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYS++IYAR+PSKALDLQSKGALLV
Sbjct: 1   METPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSVTIYARSPSKALDLQSKGALLV 60

Query: 61  NSVLQLAQTSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120
           NSVL+LAQTSDIVFTMVGHPSDVRQNVLD+NGILQGLNPGGVIVDTTSSHPALAR+IF A
Sbjct: 61  NSVLELAQTSDIVFTMVGHPSDVRQNVLDDNGILQGLNPGGVIVDTTSSHPALARNIFTA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLF+VLGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVGEAGCGQS 180

Query: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240
           CKIANQ  ISANL+GLSEGL+FAERAGLD KKF+EAVRGG A SMAMELF ERMIGRDFR
Sbjct: 181 CKIANQIPISANLMGLSEGLMFAERAGLDTKKFLEAVRGGAAGSMAMELFGERMIGRDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVI 300
           PGG+AEYMVKDLGM VD+VEEG+DERVVVLPGAAL KQLFSAMVANGDGKFG+QGLISVI
Sbjct: 241 PGGYAEYMVKDLGMAVDIVEEGEDERVVVLPGAALIKQLFSAMVANGDGKFGSQGLISVI 300

Query: 301 ERLNGK 307
           ERLNGK
Sbjct: 301 ERLNGK 306

BLAST of CSPI03G18920 vs. NCBI nr
Match: KAA0039059.1 (putative 3-hydroxyisobutyrate dehydrogenase-like 3 [Cucumis melo var. makuwa])

HSP 1 Score: 563.9 bits (1452), Expect = 8.4e-157
Identity = 281/305 (92.13%), Postives = 296/305 (97.05%), Query Frame = 0

Query: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60
           M+TPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYS++IYAR+PSKALDLQSKGALLV
Sbjct: 1   METPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSVTIYARSPSKALDLQSKGALLV 60

Query: 61  NSVLQLAQTSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120
           NSVL+LAQTSDIVFTMVGHPSDVRQNVLD+NGILQGLNPGGVIVDTTSSHPALAR+IF A
Sbjct: 61  NSVLELAQTSDIVFTMVGHPSDVRQNVLDDNGILQGLNPGGVIVDTTSSHPALARNIFTA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLF+VLGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVGEAGCGQS 180

Query: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240
           CKIANQ  ISANL+GLSEGL+FAERAGLD KKF+EAVRGG A SMAMELF ERMIGRDFR
Sbjct: 181 CKIANQIPISANLMGLSEGLMFAERAGLDTKKFLEAVRGGAAGSMAMELFGERMIGRDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVI 300
           PGG+AEYMVKDLGM VD+VEEG+DERVVVLPGAAL KQLFSAMVANGDGKFG+QGLISVI
Sbjct: 241 PGGYAEYMVKDLGMAVDIVEEGEDERVVVLPGAALIKQLFSAMVANGDGKFGSQGLISVI 300

Query: 301 ERLNG 306
           ERLNG
Sbjct: 301 ERLNG 305

BLAST of CSPI03G18920 vs. NCBI nr
Match: TYJ99748.1 (putative 3-hydroxyisobutyrate dehydrogenase-like 3 [Cucumis melo var. makuwa])

HSP 1 Score: 562.0 bits (1447), Expect = 3.2e-156
Identity = 280/305 (91.80%), Postives = 295/305 (96.72%), Query Frame = 0

Query: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60
           M+TPYPNPISPTKTRIGWIGIGVMGAAMATRLL AGYS++IYAR+PSKALDLQSKGALLV
Sbjct: 1   METPYPNPISPTKTRIGWIGIGVMGAAMATRLLCAGYSVTIYARSPSKALDLQSKGALLV 60

Query: 61  NSVLQLAQTSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120
           NSVL+LAQTSDIVFTMVGHPSDVRQNVLD+NGILQGLNPGGVIVDTTSSHPALAR+IF A
Sbjct: 61  NSVLELAQTSDIVFTMVGHPSDVRQNVLDDNGILQGLNPGGVIVDTTSSHPALARNIFTA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLF+VLGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVGEAGCGQS 180

Query: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240
           CKIANQ  ISANL+GLSEGL+FAERAGLD KKF+EAVRGG A SMAMELF ERMIGRDFR
Sbjct: 181 CKIANQIPISANLMGLSEGLMFAERAGLDTKKFLEAVRGGAAGSMAMELFGERMIGRDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVI 300
           PGG+AEYMVKDLGM V++VEEG+DERVVVLPGAAL KQLFSAMVANGDGKFGNQGLISVI
Sbjct: 241 PGGYAEYMVKDLGMAVNIVEEGEDERVVVLPGAALIKQLFSAMVANGDGKFGNQGLISVI 300

Query: 301 ERLNG 306
           ERLNG
Sbjct: 301 ERLNG 305

BLAST of CSPI03G18920 vs. NCBI nr
Match: KAG7031015.1 (putative 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 537.3 bits (1383), Expect = 8.4e-149
Identity = 269/307 (87.62%), Postives = 288/307 (93.81%), Query Frame = 0

Query: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60
           M+TPYP PISPTKTRIGWIGIGVMGAAMATRLL+AGYS++IYAR+PSKALDLQSKGA LV
Sbjct: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGACLV 60

Query: 61  NSVLQLAQTSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120
           NS  +LAQTSDIVFTMVGHPSDVRQNVL++NG+LQGLNPGGVIVDTTSSHPALAR+IF A
Sbjct: 61  NSASELAQTSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGD GVV WL PLF+ LGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180

Query: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240
           CKIANQ AI  NL+GLSEGLVFAERAGLD+K+FVEAVRGG A SMAMELF ERMI RDFR
Sbjct: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGDD-ERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISV 300
           PGGFAEYMVKDLGMGVDVVEEG+D ER+VVLPGAAL KQLFSAMVANGDGKFG QGLISV
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300

Query: 301 IERLNGK 307
           ++RLNG+
Sbjct: 301 VQRLNGR 307

BLAST of CSPI03G18920 vs. TAIR 10
Match: AT1G71180.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 365.2 bits (936), Expect = 5.3e-101
Identity = 180/305 (59.02%), Postives = 229/305 (75.08%), Query Frame = 0

Query: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60
           M+TPYP  I P+KTRIGWIGIG+MG+AM + +++AGYS+++YAR   K  DLQ+KGA + 
Sbjct: 22  METPYPKLIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIA 81

Query: 61  NSVLQLAQTSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120
           NS  +LA+ SD+VFT+VG+ +DVR  +L ++G+L GL PGGV VD TSS P LAR+I   
Sbjct: 82  NSPKELAEMSDVVFTIVGNFNDVRSLLLGDDGVLSGLTPGGVTVDMTSSKPGLAREIHAE 141

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180
           AR + CWAVDAPVSGGD GAR+G L IF GGD  +V WL P+ + +G VTY+GEAG GQS
Sbjct: 142 ARRRNCWAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQS 201

Query: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240
           CKI NQ A ++NL+GL+EG+VFAE+AGLD  K++EAV+ G A S  M LF E ++ RD+R
Sbjct: 202 CKIGNQIAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYR 261

Query: 241 PGGFAEYMVKDLGMGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVI 300
             GFAEYMVKDLGM  +            +PGAAL+KQLF+ MVANGDGK G QG++SVI
Sbjct: 262 ATGFAEYMVKDLGMAAE----------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSVI 316

Query: 301 ERLNG 306
            RLNG
Sbjct: 322 RRLNG 316

BLAST of CSPI03G18920 vs. TAIR 10
Match: AT1G71170.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 354.8 bits (909), Expect = 7.2e-98
Identity = 173/305 (56.72%), Postives = 221/305 (72.46%), Query Frame = 0

Query: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60
           M+T YP  I P+KTRIGWIGIG+MG+AM + +L+AGYS+++YAR   K  DLQ+KG    
Sbjct: 1   METQYPKLIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTA 60

Query: 61  NSVLQLAQTSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120
           NS  +L + SD+VFT+VG+ +DVR  +L ++G+L GL PGGV VD TSS P LAR+I+  
Sbjct: 61  NSPKELGEMSDVVFTIVGNSNDVRSLLLGDDGVLSGLKPGGVTVDMTSSKPGLAREIYAE 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180
           AR + CWAVDAPVSGGD GAR+GKL IF GGD  +V WL P+ + +G V ++G AG GQS
Sbjct: 121 ARRRDCWAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQS 180

Query: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240
           CKI NQ  + +N++GL+EG+VFAE+AGLD  K++EAV+ G A S  M LF E M  RD++
Sbjct: 181 CKIGNQICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYK 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVI 300
             GFAEYMVKDLGM          E  + +PG AL KQLF+ MVANGDGK G QG++ VI
Sbjct: 241 ATGFAEYMVKDLGMAA--------EAAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDVI 297

Query: 301 ERLNG 306
            RLNG
Sbjct: 301 RRLNG 297

BLAST of CSPI03G18920 vs. TAIR 10
Match: AT4G29120.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 286.6 bits (732), Expect = 2.4e-77
Identity = 144/297 (48.48%), Postives = 212/297 (71.38%), Query Frame = 0

Query: 9   ISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLVNSVLQLAQ 68
           I+P+ T+IGWIG GVMG +M   L+ AGY+++++ RT SKA  L   GA + +S   +A+
Sbjct: 33  ITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNRTISKAQTLIDMGANVADSPNSVAE 92

Query: 69  TSDIVFTMVGHPSDVRQNVLD-ENGILQGLNPGGVIVDTTSSHPALARDIFIAARAKGCW 128
            SD+VFT+VG+PSDVR  +LD ++G L GL  GGV+VD T+S P+LA +I  AA  K C+
Sbjct: 93  QSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVLVDMTTSEPSLAEEIAKAASFKNCF 152

Query: 129 AVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQSCKIANQF 188
           ++DAPVSGGD+GA++GKL+IF GGDE  V  L PLF ++GKV ++G +G GQ  K+ANQ 
Sbjct: 153 SIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLFSLMGKVNFMGTSGKGQFAKLANQI 212

Query: 189 AISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFRPGGFAEY 248
            I++ +LGL EGL++A +AGLD+KKF+EA+  G A S +++L+ +R++ RDF PG +  +
Sbjct: 213 TIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAGSKSIDLYGDRILKRDFDPGFYVNH 272

Query: 249 MVKDLGMGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVIERLN 305
            VKDLG+ ++  +       + LPG AL +QL+ ++ A+G+G  G Q L+  +ERLN
Sbjct: 273 FVKDLGICLNECQ----RMGLALPGLALAQQLYLSLKAHGEGDLGTQALLLALERLN 325

BLAST of CSPI03G18920 vs. TAIR 10
Match: AT3G25530.1 (glyoxylate reductase 1 )

HSP 1 Score: 112.8 bits (281), Expect = 4.8e-25
Identity = 76/287 (26.48%), Postives = 134/287 (46.69%), Query Frame = 0

Query: 16  IGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLVNSVLQLAQTSDIVFT 75
           +G++G+G+MG AM+  LL  G+ ++++ RT SK  +L   GA +  S  ++ +       
Sbjct: 3   VGFLGLGIMGKAMSMNLLKNGFKVTVWNRTLSKCDELVEHGASVCESPAEVIKKCKYTIA 62

Query: 76  MVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIAARAKGCWAVDAPVSG 135
           M+  P      V D+ G+L+ +  G   +D ++     +  I  A   KG   V+ PVSG
Sbjct: 63  MLSDPCAALSVVFDKGGVLEQICEGKGYIDMSTVDAETSLKINEAITGKGGRFVEGPVSG 122

Query: 136 GDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVT-YVGEAGCGQSCKIANQFAISANLL 195
               A DG+L I   GD+ +    +P F VLGK + Y+G+ G G   K+     + + + 
Sbjct: 123 SKKPAEDGQLIILAAGDKALFEESIPAFDVLGKRSFYLGQVGNGAKMKLIVNMIMGSMMN 182

Query: 196 GLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFRPGGFAEYMVKDLGM 255
             SEGLV A+++GL     ++ +  G   +   +     M    + P    ++  KD+ +
Sbjct: 183 AFSEGLVLADKSGLSSDTLLDILDLGAMTNPMFKGKGPSMNKSSYPPAFPLKHQQKDMRL 242

Query: 256 GVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVIE 302
            + +     DE  V +P AA   + F    + G G      +I  ++
Sbjct: 243 ALAL----GDENAVSMPVAAAANEAFKKARSLGLGDLDFSAVIEAVK 285

BLAST of CSPI03G18920 vs. TAIR 10
Match: AT1G17650.1 (glyoxylate reductase 2 )

HSP 1 Score: 110.2 bits (274), Expect = 3.1e-24
Identity = 79/291 (27.15%), Postives = 136/291 (46.74%), Query Frame = 0

Query: 16  IGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLVNSVLQLAQTSDIVFT 75
           IG++G+G+MG+ MA  L+ AG  ++++ RT SK   L   GA   +S  ++  T D+ F 
Sbjct: 69  IGFLGMGIMGSPMAQNLIKAGCDVTVWNRTKSKCDPLVGLGAKYKSSPEEVTATCDLTFA 128

Query: 76  MVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIAARAK--GCWAVDAPV 135
           M+  P         +NG + G++ G   VD ++    +A  I I+ + K  G   ++APV
Sbjct: 129 MLADPESAIDVACGKNGAIFGISSGKGYVDVSTVD--VASSILISKQIKDTGALFLEAPV 188

Query: 136 SGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVT-YVGEAGCGQSCKIANQFAISAN 195
           SG    A DG+L     GD+ +     P   ++GK   Y+GE G G + K+     + + 
Sbjct: 189 SGSKKPAEDGQLIFLTAGDKPLYEKAAPFLDIMGKSKFYLGEVGNGAAMKLVVNMIMGSM 248

Query: 196 LLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFRPGGFAEYMVKDL 255
           +   +EG++ +++ GLD    VE V  G   +    L    MI   +      ++  KD+
Sbjct: 249 MASFAEGILLSQKVGLDPNVLVEVVSQGAINAPMYSLKGPSMIKSVYPTAFPLKHQQKDM 308

Query: 256 GMGVDVVEEGDDERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISVIERL 304
            + + + E          P AA   +L+    + G     ++   +VIE L
Sbjct: 309 RLALGLAESVSQS----TPIAAAANELYKVAKSYG---LSDEDFSAVIEAL 350

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949M87.5e-10059.02Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis... [more]
Q9C9911.0e-9656.72Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis... [more]
Q9SZE13.4e-7648.48Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis... [more]
O349481.1e-5537.54Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) OX=224308 ... [more]
P771614.5e-4437.852-hydroxy-3-oxopropionate reductase OS=Escherichia coli (strain K12) OX=83333 GN... [more]
Match NameE-valueIdentityDescription
A0A0A0L7521.8e-16899.02Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G217150 PE=4 SV=1[more]
A0A1S3C3N41.1e-15792.16probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Cucumis mel... [more]
A0A5A7TCQ04.1e-15792.13Putative 3-hydroxyisobutyrate dehydrogenase-like 3 OS=Cucumis melo var. makuwa O... [more]
A0A5D3BNN21.5e-15691.80Putative 3-hydroxyisobutyrate dehydrogenase-like 3 OS=Cucumis melo var. makuwa O... [more]
A0A0A0LC064.1e-14997.46Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G214010 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_004149969.34.3e-16999.67probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucumis sativ... [more]
XP_008456080.12.2e-15792.16PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cu... [more]
KAA0039059.18.4e-15792.13putative 3-hydroxyisobutyrate dehydrogenase-like 3 [Cucumis melo var. makuwa][more]
TYJ99748.13.2e-15691.80putative 3-hydroxyisobutyrate dehydrogenase-like 3 [Cucumis melo var. makuwa][more]
KAG7031015.18.4e-14987.62putative 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial, partial [Cucu... [more]
Match NameE-valueIdentityDescription
AT1G71180.15.3e-10159.026-phosphogluconate dehydrogenase family protein [more]
AT1G71170.17.2e-9856.726-phosphogluconate dehydrogenase family protein [more]
AT4G29120.12.4e-7748.486-phosphogluconate dehydrogenase family protein [more]
AT3G25530.14.8e-2526.48glyoxylate reductase 1 [more]
AT1G17650.13.1e-2427.15glyoxylate reductase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 11..173
e-value: 1.2E-43
score: 150.8
NoneNo IPR availablePANTHERPTHR430603-HYDROXYISOBUTYRATE DEHYDROGENASE-LIKE 1, MITOCHONDRIAL-RELATEDcoord: 1..306
NoneNo IPR availablePANTHERPTHR43060:SF133-HYDROXYISOBUTYRATE DEHYDROGENASE-LIKE 2, MITOCHONDRIAL-RELATEDcoord: 1..306
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 174..306
e-value: 6.9E-34
score: 118.9
IPR0061156-phosphogluconate dehydrogenase, NADP-bindingPFAMPF03446NAD_binding_2coord: 15..173
e-value: 8.4E-40
score: 136.5
IPR0291543-hydroxyisobutyrate dehydrogenase, NAD-binding domainPFAMPF14833NAD_binding_11coord: 176..298
e-value: 2.3E-23
score: 82.7
IPR0158153-hydroxyisobutyrate dehydrogenase-relatedPIRSFPIRSF000103HIBADHcoord: 10..306
e-value: 5.2E-53
score: 178.0
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 14..172
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 174..305

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G18920.1CSPI03G18920.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0051287 NAD binding
molecular_function GO:0050661 NADP binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0016491 oxidoreductase activity