CSPI03G12740 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G12740
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionstem-specific protein TSJT1
LocationChr3: 9717163 .. 9719462 (-)
RNA-Seq ExpressionCSPI03G12740
SyntenyCSPI03G12740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATATATTACGTATTATTTTAATTTTTAAGTATCTCTAAACTCCAGGTCCTCCGTCTTCCAACTGCAATCGATCTTTTTTTCATCATCTGTTTTGCATTCAGAAACTAAACCAACCGTGTTTTTAGTTTTCTTATTTTCCGATCGACGATCTACAAAATGTTCGCCGTCTTCGACAAATCTGTGGCCAAAAGTCCCGACGCTCTGCAGAGCCCTGAGTCCGATTCCACTTGGGCTCTCAAAGATGGAATTCTGGCTCAGCATTTTAGCTCCGTTTATCCTGGTTCTGTCATCGTCAACCTCGGCTCTTCCGGTCTCTTGGCCTATTCCGTCGAAAAACAGAACCCTATCCTCCCAAGGTATTACCATTACCCTTTCATCTTCTTCTGCCAATGTTTTCTGGAAAACTCTAATATTCACACGCTTTTTGTTTTTTAATTTTTATTATTTTTGTTTCTTTCTTTTTCTATATTCTTTCTGGATCTGATCACTGATCTTGTTTACTTATTGTCTCCCCGAGGTTTCTTCTATTGGGGTTTTCATTTTGGTTGGATGAGAATCTGTTTAAACTGAGAAGCATCGAGTTTTCTTTGTTTATTGATCCCTATTTTGTTCTTAGCTATGAAATCACGTCGTATGTTTTTGTGATGGTTGTGTATTTGGTGAGGAAATGGTTGCACTCTTTCAGGCCATTATTTTGTCCTTTTCTGTTTTGCTTGAGTTTTTCCATTTTGATTCGATCGAAATAATGGAGGGTCAAATCTTTCGAGATTTGAAATGATTTTTGTTCTTGTTTTTCATCCTCAACCAAAACTATGGGATTCTTGGATTGGCTTATTCTTTTGGACTTTATCAATAATCTTAATTGCCATGGGATTTGTGTTGAATCGTTGGTAAGAGAGTTGCGGGTTGTTGGCAAGTATGAACATTTGTTAAGTTTCCCTGCTGTTGGCAAATTTGATGAAATTTATTTCTGTAACCTTAGATTATTTGCGGTTGTGGACGACATTTTCTGCTTGTTCCAAGGGCACATTGAGAATGTTGCTCAACTCAAACAACAATACGGTCTGAACAAAGCTGCAAACGAGGTTATAATCGTTATTGAAGCCTATCGAACATTAAGAGATCGGGGTCCATACCCAGCCGATCAGGTCGTGAGAGATATTCAAGGGAAATTTGTGTTTATCCTATACGACAGTTCCTCGAAAACTTCATTTTTTGCTTCTGTAAGTTGCCTTGTTCTCTTAGTTTAACCATGATTTGCCACAAATTTCTATTGTGATCGTTAATCTCCATTTTCTCTTAATGTCTCTTTTTATCGGCTGCAGGATGCTGATGGGAGCGTTCCCTTCCACTGGGGAACTGATTCTGAAGGACAGCTTGTTCTCTCCGATGATGTTGAAATCATGAAGAAGGGTTGTGGAAAATCTTTTGCTCCTTTCCCCAAAGGTAACCATTTGAACGAATTCATTCGGTTAAATTTTTTCTACTTCTATCACCATTTGGCAATTGTCCTTTCCAAAATAAGTCAATTGCAAGGTTGAAGTATTGAACAATCTAACTTTTGAGATAGTAATTTATGTCTTATCATTGAGCTATGCTCAAATTGAAGTTTACTAGAATCGAATATGTTTCTGATCATTTATATTATGCAATATGTTTGGTATTTTAGGTTGTTTCTTCACAACTTCTGGTGGGTTAAGGAGTTATGAGCATCCACTTAATGAGCTGAAGCCAGTGCCTAGGGTGGACAGTTCAGGTAATGTCTGTGGAGCCAATTTCAAGGTGGACGCTGAGGCTCGAAAGGAAAGTTCTGGTATGCCAAGAGTTGGGAGTGCTGCAAATTGGTCTTCAAACTACTGAGTGCATGATTCGGCTTACTTTGTCTTAGCCTTCCTACGGCTTCTTTCCATATTTTCATCTTTTATTCCTCTAATTTGTGCCTTTTAAATGGGTACCTGAGGTTTATACTTTTACTTTCCGTAATGGGCATGTTCATGCAGTTACAACTTATATCTTATGAATCTGTTGGCTTAATTGAGTTACATTCTGGTTACGATGGAATAAGAGTTTGTTTTCCTAATGCTTTGTCAACTTCTATATTTTGATGAAGGGTTTTGATCCCAACAGAATTCACTGTAGTTTTGGATTTATGCCAATAAAGTTTCTCCTTGCCAACTACATATTTGGCTTTATCTTAGTTCAACTGAGATAT

mRNA sequence

AAATATATTACGTATTATTTTAATTTTTAAGTATCTCTAAACTCCAGGTCCTCCGTCTTCCAACTGCAATCGATCTTTTTTTCATCATCTGTTTTGCATTCAGAAACTAAACCAACCGTGTTTTTAGTTTTCTTATTTTCCGATCGACGATCTACAAAATGTTCGCCGTCTTCGACAAATCTGTGGCCAAAAGTCCCGACGCTCTGCAGAGCCCTGAGTCCGATTCCACTTGGGCTCTCAAAGATGGAATTCTGGCTCAGCATTTTAGCTCCGTTTATCCTGGTTCTGTCATCGTCAACCTCGGCTCTTCCGGTCTCTTGGCCTATTCCGTCGAAAAACAGAACCCTATCCTCCCAAGATTATTTGCGGTTGTGGACGACATTTTCTGCTTGTTCCAAGGGCACATTGAGAATGTTGCTCAACTCAAACAACAATACGGTCTGAACAAAGCTGCAAACGAGGTTATAATCGTTATTGAAGCCTATCGAACATTAAGAGATCGGGGTCCATACCCAGCCGATCAGGTCGTGAGAGATATTCAAGGGAAATTTGTGTTTATCCTATACGACAGTTCCTCGAAAACTTCATTTTTTGCTTCTGATGCTGATGGGAGCGTTCCCTTCCACTGGGGAACTGATTCTGAAGGACAGCTTGTTCTCTCCGATGATGTTGAAATCATGAAGAAGGGTTGTGGAAAATCTTTTGCTCCTTTCCCCAAAGGTTGTTTCTTCACAACTTCTGGTGGGTTAAGGAGTTATGAGCATCCACTTAATGAGCTGAAGCCAGTGCCTAGGGTGGACAGTTCAGGTAATGTCTGTGGAGCCAATTTCAAGGTGGACGCTGAGGCTCGAAAGGAAAGTTCTGGTATGCCAAGAGTTGGGAGTGCTGCAAATTGGTCTTCAAACTACTGAGTGCATGATTCGGCTTACTTTGTCTTAGCCTTCCTACGGCTTCTTTCCATATTTTCATCTTTTATTCCTCTAATTTGTGCCTTTTAAATGGGTACCTGAGGTTTATACTTTTACTTTCCGTAATGGGCATGTTCATGCAGTTACAACTTATATCTTATGAATCTGTTGGCTTAATTGAGTTACATTCTGGTTACGATGGAATAAGAGTTTGTTTTCCTAATGCTTTGTCAACTTCTATATTTTGATGAAGGGTTTTGATCCCAACAGAATTCACTGTAGTTTTGGATTTATGCCAATAAAGTTTCTCCTTGCCAACTACATATTTGGCTTTATCTTAGTTCAACTGAGATAT

Coding sequence (CDS)

ATGTTCGCCGTCTTCGACAAATCTGTGGCCAAAAGTCCCGACGCTCTGCAGAGCCCTGAGTCCGATTCCACTTGGGCTCTCAAAGATGGAATTCTGGCTCAGCATTTTAGCTCCGTTTATCCTGGTTCTGTCATCGTCAACCTCGGCTCTTCCGGTCTCTTGGCCTATTCCGTCGAAAAACAGAACCCTATCCTCCCAAGATTATTTGCGGTTGTGGACGACATTTTCTGCTTGTTCCAAGGGCACATTGAGAATGTTGCTCAACTCAAACAACAATACGGTCTGAACAAAGCTGCAAACGAGGTTATAATCGTTATTGAAGCCTATCGAACATTAAGAGATCGGGGTCCATACCCAGCCGATCAGGTCGTGAGAGATATTCAAGGGAAATTTGTGTTTATCCTATACGACAGTTCCTCGAAAACTTCATTTTTTGCTTCTGATGCTGATGGGAGCGTTCCCTTCCACTGGGGAACTGATTCTGAAGGACAGCTTGTTCTCTCCGATGATGTTGAAATCATGAAGAAGGGTTGTGGAAAATCTTTTGCTCCTTTCCCCAAAGGTTGTTTCTTCACAACTTCTGGTGGGTTAAGGAGTTATGAGCATCCACTTAATGAGCTGAAGCCAGTGCCTAGGGTGGACAGTTCAGGTAATGTCTGTGGAGCCAATTTCAAGGTGGACGCTGAGGCTCGAAAGGAAAGTTCTGGTATGCCAAGAGTTGGGAGTGCTGCAAATTGGTCTTCAAACTACTGA

Protein sequence

MFAVFDKSVAKSPDALQSPESDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSVEKQNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPADQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGKSFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRVGSAANWSSNY*
Homology
BLAST of CSPI03G12740 vs. ExPASy Swiss-Prot
Match: P24805 (Stem-specific protein TSJT1 OS=Nicotiana tabacum OX=4097 GN=TSJT1 PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.1e-28
Identity = 58/148 (39.19%), Postives = 93/148 (62.84%), Query Frame = 0

Query: 1   MFAVFDKSVAKSPDALQSPES--DSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSV 60
           M AVF++S+ + P  L  P++      A     +A+ F +    S   +L +   +A+S 
Sbjct: 1   MLAVFEQSIGRPPPELSLPQAGIQKKEAKTREEIAESFKTWKQDSTFYHLFNGNFMAFSH 60

Query: 61  EKQNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPY 120
             +NP+ PR   V+DD+FC+F G ++N   L++ YGL++ A E +I++EAY+ LRDR PY
Sbjct: 61  GNENPLQPRSIVVMDDVFCIFSGALDNTFDLRKHYGLSRQATEAMIMVEAYKVLRDRAPY 120

Query: 121 PADQVVRDIQGKFVFILYDSSSKTSFFA 147
           P DQV+++++GKF FIL+DS + T F A
Sbjct: 121 PPDQVIKELEGKFAFILFDSKASTLFLA 148

BLAST of CSPI03G12740 vs. ExPASy Swiss-Prot
Match: P22106 (Asparagine synthetase B [glutamine-hydrolyzing] OS=Escherichia coli (strain K12) OX=83333 GN=asnB PE=1 SV=3)

HSP 1 Score: 60.1 bits (144), Expect = 4.2e-08
Identity = 35/126 (27.78%), Postives = 63/126 (50.00%), Query Frame = 0

Query: 81  GHIENVAQLKQQYGLN---KAANEVIIVIEAYRTLRDRGPYPADQVVRDIQGKFVFILYD 140
           G I N   L+ +YG     +  ++  +++  Y   +++GP    + + D+QG F F LYD
Sbjct: 76  GEIYNHQALRAEYGDRYQFQTGSDCEVILALY---QEKGP----EFLDDLQGMFAFALYD 135

Query: 141 SSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGKSFAPFPKGCF-FTTSGG 200
           S         D  G +P + G D  GQL ++ +++ +   C ++   FP G + ++  G 
Sbjct: 136 SEKDAYLIGRDHLGIIPLYMGYDEHGQLYVASEMKALVPVC-RTIKEFPAGSYLWSQDGE 193

Query: 201 LRSYEH 203
           +RSY H
Sbjct: 196 IRSYYH 193

BLAST of CSPI03G12740 vs. ExPASy Swiss-Prot
Match: Q10MX3 (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0291500 PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.8e-06
Identity = 35/133 (26.32%), Postives = 64/133 (48.12%), Query Frame = 0

Query: 75  IFCLFQGHIENVAQLKQQYGLN---KAANEVIIVIEAYRTLRDRGPYPADQVVRDIQGKF 134
           I  +  G I N  ++++Q+         ++  ++I  Y           +  V  + G F
Sbjct: 70  IVVVANGEIYNHKKIRKQFASKHTFSTGSDCEVIIPLYEEY-------GEDFVDMLDGVF 129

Query: 135 VFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGKSFAPFPKGCFF 194
            F+LYD+ +KT   A DA G  P + G  S+G + +S +++ + + C   F  FP G  +
Sbjct: 130 AFVLYDTRTKTYMAARDAIGVNPLYIGRGSDGAVWISSEMKALNEDC-VEFEIFPPGHLY 189

Query: 195 TT-SGGLRSYEHP 204
           ++ +GGLR +  P
Sbjct: 190 SSAAGGLRRWYKP 194

BLAST of CSPI03G12740 vs. ExPASy Swiss-Prot
Match: Q43011 (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0265000 PE=2 SV=3)

HSP 1 Score: 50.4 bits (119), Expect = 3.3e-05
Identity = 33/126 (26.19%), Postives = 59/126 (46.83%), Query Frame = 0

Query: 81  GHIENVAQLKQQYGLNK--AANEVIIVIEAYRTLRDRGPYPADQVVRDIQGKFVFILYDS 140
           G I N  +LK     +K   A++  ++   Y           ++ V  + G F F+L D+
Sbjct: 76  GEIYNHEELKANLKSHKFQTASDCEVIAHLYEEY-------GEEFVDMLDGMFAFVLLDT 135

Query: 141 SSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGKSFAPFPKGCFFTT-SGGL 200
             K+   A DA G  P + G   +G +  S +++ +   C + F  FP G  +++ +GGL
Sbjct: 136 RDKSFIAARDAIGICPLYMGWGLDGSVWFSSEMKALSDDC-ERFISFPPGHLYSSKTGGL 193

Query: 201 RSYEHP 204
           R + +P
Sbjct: 196 RRWYNP 193

BLAST of CSPI03G12740 vs. ExPASy Swiss-Prot
Match: Q54MB4 (Probable asparagine synthetase [glutamine-hydrolyzing] OS=Dictyostelium discoideum OX=44689 GN=asns PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 7.4e-05
Identity = 23/73 (31.51%), Postives = 39/73 (53.42%), Query Frame = 0

Query: 121 DQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180
           D  V  + G F F++Y+  + +   A D  G VP + G   +G +  S +++ +K  C K
Sbjct: 114 DDFVHMLDGDFAFVVYNKKANSFLAARDPIGVVPLYIGWHKDGSIWFSSEMKAIKDDCYK 173

Query: 181 SFAPFPKGCFFTT 194
            F PFP G +F++
Sbjct: 174 -FQPFPPGHYFSS 185

BLAST of CSPI03G12740 vs. ExPASy TrEMBL
Match: A0A0A0L7D3 (DUF3700 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G145760 PE=4 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.5e-141
Identity = 250/250 (100.00%), Postives = 250/250 (100.00%), Query Frame = 0

Query: 1   MFAVFDKSVAKSPDALQSPESDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSVEK 60
           MFAVFDKSVAKSPDALQSPESDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSVEK
Sbjct: 1   MFAVFDKSVAKSPDALQSPESDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSVEK 60

Query: 61  QNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           QNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  QNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180
           DQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK
Sbjct: 121 DQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240
           SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV
Sbjct: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240

Query: 241 GSAANWSSNY 251
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of CSPI03G12740 vs. ExPASy TrEMBL
Match: A0A5D3CZ34 (Stem-specific protein TSJT1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00820 PE=4 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.1e-139
Identity = 244/250 (97.60%), Postives = 248/250 (99.20%), Query Frame = 0

Query: 1   MFAVFDKSVAKSPDALQSPESDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSVEK 60
           M AVF+KSVAKSPDALQSPESDSTWALKDGILAQHFSS YPGSVIVNLGS+GLLAYSVEK
Sbjct: 1   MLAVFEKSVAKSPDALQSPESDSTWALKDGILAQHFSSAYPGSVIVNLGSAGLLAYSVEK 60

Query: 61  QNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           QNP+LPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180
           DQVVRDIQGKFVF+LYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK
Sbjct: 121 DQVVRDIQGKFVFVLYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240
           SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV
Sbjct: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240

Query: 241 GSAANWSSNY 251
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of CSPI03G12740 vs. ExPASy TrEMBL
Match: A0A1S3AXH1 (stem-specific protein TSJT1 OS=Cucumis melo OX=3656 GN=LOC103483669 PE=4 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.1e-139
Identity = 244/250 (97.60%), Postives = 248/250 (99.20%), Query Frame = 0

Query: 1   MFAVFDKSVAKSPDALQSPESDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSVEK 60
           M AVF+KSVAKSPDALQSPESDSTWALKDGILAQHFSS YPGSVIVNLGS+GLLAYSVEK
Sbjct: 1   MLAVFEKSVAKSPDALQSPESDSTWALKDGILAQHFSSAYPGSVIVNLGSAGLLAYSVEK 60

Query: 61  QNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           QNP+LPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180
           DQVVRDIQGKFVF+LYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK
Sbjct: 121 DQVVRDIQGKFVFVLYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240
           SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV
Sbjct: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240

Query: 241 GSAANWSSNY 251
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of CSPI03G12740 vs. ExPASy TrEMBL
Match: A0A6J1IG81 (stem-specific protein TSJT1-like OS=Cucurbita maxima OX=3661 GN=LOC111473103 PE=4 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.3e-132
Identity = 233/250 (93.20%), Postives = 242/250 (96.80%), Query Frame = 0

Query: 1   MFAVFDKSVAKSPDALQSPESDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSVEK 60
           M AVFDKSVAKSPDALQSP SDS WALKDGILA+HFSSVYP SVIVNLGS+GLLAYSVEK
Sbjct: 1   MLAVFDKSVAKSPDALQSPHSDSVWALKDGILAKHFSSVYPDSVIVNLGSAGLLAYSVEK 60

Query: 61  QNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           +NP+LPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  KNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180
           DQVVRDIQGKFVF+L+DSSSK +FFASDADGSVP HWGTDSEGQLVLSDDVEIMKKGCGK
Sbjct: 121 DQVVRDIQGKFVFVLFDSSSKAAFFASDADGSVPLHWGTDSEGQLVLSDDVEIMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240
           SFA FPKGCFFTTSGGLRS+EHPLNELKPVPRVDSSG+VCGANFKVD EARKESSGMPRV
Sbjct: 181 SFASFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240

Query: 241 GSAANWSSNY 251
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of CSPI03G12740 vs. ExPASy TrEMBL
Match: A0A6J1G7B2 (stem-specific protein TSJT1-like OS=Cucurbita moschata OX=3662 GN=LOC111451479 PE=4 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.3e-132
Identity = 233/250 (93.20%), Postives = 242/250 (96.80%), Query Frame = 0

Query: 1   MFAVFDKSVAKSPDALQSPESDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSVEK 60
           M AVFDKSVAKSPDALQSP SDS WALKDGILA+HFSSVYP SVIVNLGS+GLLAYSVEK
Sbjct: 1   MLAVFDKSVAKSPDALQSPHSDSVWALKDGILAKHFSSVYPDSVIVNLGSAGLLAYSVEK 60

Query: 61  QNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           +NP+LPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  KNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180
           DQVVRDIQGKFVF+L+DSSSK +FFASDADGSVP HWGTDSEGQLVLSDDVEIMKKGCGK
Sbjct: 121 DQVVRDIQGKFVFVLFDSSSKAAFFASDADGSVPLHWGTDSEGQLVLSDDVEIMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240
           SFA FPKGCFFTTSGGLRS+EHPLNELKPVPRVDSSG+VCGANFKVD EARKESSGMPRV
Sbjct: 181 SFASFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240

Query: 241 GSAANWSSNY 251
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of CSPI03G12740 vs. NCBI nr
Match: XP_004134117.1 (stem-specific protein TSJT1 [Cucumis sativus] >KGN56939.1 hypothetical protein Csa_011293 [Cucumis sativus])

HSP 1 Score: 511.9 bits (1317), Expect = 3.1e-141
Identity = 250/250 (100.00%), Postives = 250/250 (100.00%), Query Frame = 0

Query: 1   MFAVFDKSVAKSPDALQSPESDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSVEK 60
           MFAVFDKSVAKSPDALQSPESDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSVEK
Sbjct: 1   MFAVFDKSVAKSPDALQSPESDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSVEK 60

Query: 61  QNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           QNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  QNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180
           DQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK
Sbjct: 121 DQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240
           SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV
Sbjct: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240

Query: 241 GSAANWSSNY 251
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of CSPI03G12740 vs. NCBI nr
Match: XP_008438612.1 (PREDICTED: stem-specific protein TSJT1 [Cucumis melo] >KAA0049327.1 stem-specific protein TSJT1 [Cucumis melo var. makuwa] >TYK17231.1 stem-specific protein TSJT1 [Cucumis melo var. makuwa])

HSP 1 Score: 504.2 bits (1297), Expect = 6.4e-139
Identity = 244/250 (97.60%), Postives = 248/250 (99.20%), Query Frame = 0

Query: 1   MFAVFDKSVAKSPDALQSPESDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSVEK 60
           M AVF+KSVAKSPDALQSPESDSTWALKDGILAQHFSS YPGSVIVNLGS+GLLAYSVEK
Sbjct: 1   MLAVFEKSVAKSPDALQSPESDSTWALKDGILAQHFSSAYPGSVIVNLGSAGLLAYSVEK 60

Query: 61  QNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           QNP+LPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180
           DQVVRDIQGKFVF+LYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK
Sbjct: 121 DQVVRDIQGKFVFVLYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240
           SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV
Sbjct: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240

Query: 241 GSAANWSSNY 251
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of CSPI03G12740 vs. NCBI nr
Match: XP_038878034.1 (stem-specific protein TSJT1 [Benincasa hispida])

HSP 1 Score: 502.3 bits (1292), Expect = 2.4e-138
Identity = 243/250 (97.20%), Postives = 247/250 (98.80%), Query Frame = 0

Query: 1   MFAVFDKSVAKSPDALQSPESDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSVEK 60
           M AVFDKSVAKSPDALQSPESDS WALKDGILAQHFSSVYPGSVIVNLGS+GLLAYSVEK
Sbjct: 1   MLAVFDKSVAKSPDALQSPESDSVWALKDGILAQHFSSVYPGSVIVNLGSAGLLAYSVEK 60

Query: 61  QNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           QNP+LPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180
           DQVVRDIQGKFVF+LYDSSSKT+FFASDADGSVPFHWGTDSEGQLVLSDDVE MKKGCGK
Sbjct: 121 DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPFHWGTDSEGQLVLSDDVETMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240
           SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV
Sbjct: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240

Query: 241 GSAANWSSNY 251
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of CSPI03G12740 vs. NCBI nr
Match: XP_022947693.1 (stem-specific protein TSJT1-like [Cucurbita moschata] >XP_022974438.1 stem-specific protein TSJT1-like [Cucurbita maxima] >XP_023540849.1 stem-specific protein TSJT1-like [Cucurbita pepo subsp. pepo] >KAG6597414.1 Stem-specific protein TSJT1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 482.3 bits (1240), Expect = 2.6e-132
Identity = 233/250 (93.20%), Postives = 242/250 (96.80%), Query Frame = 0

Query: 1   MFAVFDKSVAKSPDALQSPESDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSVEK 60
           M AVFDKSVAKSPDALQSP SDS WALKDGILA+HFSSVYP SVIVNLGS+GLLAYSVEK
Sbjct: 1   MLAVFDKSVAKSPDALQSPHSDSVWALKDGILAKHFSSVYPDSVIVNLGSAGLLAYSVEK 60

Query: 61  QNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           +NP+LPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  KNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180
           DQVVRDIQGKFVF+L+DSSSK +FFASDADGSVP HWGTDSEGQLVLSDDVEIMKKGCGK
Sbjct: 121 DQVVRDIQGKFVFVLFDSSSKAAFFASDADGSVPLHWGTDSEGQLVLSDDVEIMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240
           SFA FPKGCFFTTSGGLRS+EHPLNELKPVPRVDSSG+VCGANFKVD EARKESSGMPRV
Sbjct: 181 SFASFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240

Query: 241 GSAANWSSNY 251
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of CSPI03G12740 vs. NCBI nr
Match: KAG7028873.1 (Stem-specific protein TSJT1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 482.3 bits (1240), Expect = 2.6e-132
Identity = 233/250 (93.20%), Postives = 242/250 (96.80%), Query Frame = 0

Query: 1   MFAVFDKSVAKSPDALQSPESDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSVEK 60
           M AVFDKSVAKSPDALQSP SDS WALKDGILA+HFSSVYP SVIVNLGS+GLLAYSVEK
Sbjct: 54  MLAVFDKSVAKSPDALQSPHSDSVWALKDGILAKHFSSVYPDSVIVNLGSAGLLAYSVEK 113

Query: 61  QNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           +NP+LPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 114 KNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 173

Query: 121 DQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180
           DQVVRDIQGKFVF+L+DSSSK +FFASDADGSVP HWGTDSEGQLVLSDDVEIMKKGCGK
Sbjct: 174 DQVVRDIQGKFVFVLFDSSSKAAFFASDADGSVPLHWGTDSEGQLVLSDDVEIMKKGCGK 233

Query: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240
           SFA FPKGCFFTTSGGLRS+EHPLNELKPVPRVDSSG+VCGANFKVD EARKESSGMPRV
Sbjct: 234 SFASFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 293

Query: 241 GSAANWSSNY 251
           GSAANWSSNY
Sbjct: 294 GSAANWSSNY 303

BLAST of CSPI03G12740 vs. TAIR 10
Match: AT3G22850.1 (Aluminium induced protein with YGL and LRDR motifs )

HSP 1 Score: 386.3 bits (991), Expect = 1.8e-107
Identity = 184/247 (74.49%), Postives = 207/247 (83.81%), Query Frame = 0

Query: 1   MFAVFDKSVAKSPDALQSPESDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSVEK 60
           M A+FDK+VAK+P+ALQ  E  S  ALKD  L  HFSSVYPG+V +NLGSSG +A S+EK
Sbjct: 1   MLAIFDKNVAKTPEALQGQEGGSVCALKDRFLPNHFSSVYPGAVTINLGSSGFIACSLEK 60

Query: 61  QNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           QNP+LPRLFAVVDD+FC+FQGHIENV  LKQQYGL K A EV IVIEAYRTLRDRGPY A
Sbjct: 61  QNPLLPRLFAVVDDMFCIFQGHIENVPILKQQYGLTKTATEVTIVIEAYRTLRDRGPYSA 120

Query: 121 DQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180
           +QVVRD QGKF F+LYD S++  F A D DGSVP +WGTD+EG LV+SDDVE +KKGCGK
Sbjct: 121 EQVVRDFQGKFGFMLYDCSTQNVFLAGDVDGSVPLYWGTDAEGHLVVSDDVETVKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240
           SFAPFPKGCFFT+SGGLRSYEHP NELKPVPRVDSSG VCG  FKVD+EA+KE+  MPRV
Sbjct: 181 SFAPFPKGCFFTSSGGLRSYEHPSNELKPVPRVDSSGEVCGVTFKVDSEAKKEA--MPRV 240

Query: 241 GSAANWS 248
           GS  NWS
Sbjct: 241 GSVQNWS 245

BLAST of CSPI03G12740 vs. TAIR 10
Match: AT5G43830.1 (Aluminium induced protein with YGL and LRDR motifs )

HSP 1 Score: 375.2 bits (962), Expect = 4.2e-104
Identity = 178/250 (71.20%), Postives = 208/250 (83.20%), Query Frame = 0

Query: 1   MFAVFDKSVAKSPDALQSP-ESDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYSVE 60
           M AVF+K+VA SP+ALQSP  S+S +ALKDG LA HF+SV P SV +N GSSG +AYS++
Sbjct: 1   MLAVFEKTVANSPEALQSPHSSESAFALKDGSLATHFASVNPNSVTLNFGSSGFVAYSLD 60

Query: 61  KQNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYP 120
             +P +PRLFAVVDDIFCLFQGHIEN+  LKQQYGLNK  NE IIVIEAYRTLRDRGPYP
Sbjct: 61  NPDPRVPRLFAVVDDIFCLFQGHIENLPFLKQQYGLNKITNEAIIVIEAYRTLRDRGPYP 120

Query: 121 ADQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCG 180
            D+VVRD  GKF FIL+DS  KT F A+DADGSVPF WGTD+EG LV SD+ E++KKGC 
Sbjct: 121 VDKVVRDFHGKFAFILFDSVKKTVFAAADADGSVPFFWGTDAEGHLVFSDNTEMVKKGCA 180

Query: 181 KSFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPR 240
           KS+ PFPKGCFFT+SGGLRS+EHP NELKPVPRVDSSG+VCGA FKVDAE ++E + MPR
Sbjct: 181 KSYGPFPKGCFFTSSGGLRSFEHPKNELKPVPRVDSSGDVCGATFKVDAETKREGTKMPR 240

Query: 241 VGSAANWSSN 250
           V S+ NW+ +
Sbjct: 241 VDSSQNWAGH 250

BLAST of CSPI03G12740 vs. TAIR 10
Match: AT4G27450.1 (Aluminium induced protein with YGL and LRDR motifs )

HSP 1 Score: 245.0 bits (624), Expect = 6.6e-65
Identity = 124/253 (49.01%), Postives = 166/253 (65.61%), Query Frame = 0

Query: 1   MFAVFDKSVAKSPDALQSPESDSTWALKDGILAQH----FSSVYP-GSVIVNLGSSGLLA 60
           M A+F ++ A  P+ L SP S+     K   L +     F   YP  +  ++ G + +LA
Sbjct: 1   MLAIFHEAFAHPPEELNSPASEK--CSKQPKLPEETLNDFLLRYPLNTFSMSFGQAAVLA 60

Query: 61  YSVEKQN-PILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRD 120
           Y     +  I  RLF   DDI+CLF G + N+ QL +QYGL K  NE + VIEAYRTLRD
Sbjct: 61  YVRPSASFSIHQRLFCGFDDIYCLFFGSLNNLCQLNKQYGLTKTTNEAMFVIEAYRTLRD 120

Query: 121 RGPYPADQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIM 180
           RGPYPADQVV+D+ G F F++YDS + + F A  +DG V  +WG  ++G +V+SDD++++
Sbjct: 121 RGPYPADQVVKDLDGSFSFVVYDSKAGSVFTALGSDGGVKLYWGIAADGSVVISDDLDVI 180

Query: 181 KKGCGKSFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKES 240
           K+GC KSFAPFP GC F + GGL S+EHP+N++K +PRVDS G +CGANFKVD   R  S
Sbjct: 181 KEGCAKSFAPFPTGCMFHSEGGLMSFEHPMNKIKAMPRVDSEGVLCGANFKVDVYNRVNS 240

Query: 241 SGMPRVGSAANWS 248
             +PR GS ANWS
Sbjct: 241 --IPRRGSEANWS 249

BLAST of CSPI03G12740 vs. TAIR 10
Match: AT3G15450.1 (Aluminium induced protein with YGL and LRDR motifs )

HSP 1 Score: 237.7 bits (605), Expect = 1.0e-62
Identity = 115/251 (45.82%), Postives = 165/251 (65.74%), Query Frame = 0

Query: 1   MFAVFDKSVAKSPDALQSPE---SDSTWALKDGILAQHFSSVYPGSVIVNLGSSGLLAYS 60
           M A+F K+ A  P+ L SP    S  T  L    L+   S     +  +N G S +LAY+
Sbjct: 1   MLAIFQKAFAHPPEELNSPASHFSGKTPKLPGETLSDFLSHHQNNAFSMNFGDSAVLAYA 60

Query: 61  VEKQNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLN-KAANEVIIVIEAYRTLRDRG 120
             ++  +  RLF  +D I+C+F G + N+  L +QYGL+ K +NE + VIEAYRTLRDRG
Sbjct: 61  -RQETSLRQRLFCGLDGIYCMFLGRLNNLCTLNRQYGLSGKNSNEAMFVIEAYRTLRDRG 120

Query: 121 PYPADQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKK 180
           PYPADQV+R ++G F F++YD+ + + F A  +DG    +WG   +G +V+SDD++I+K+
Sbjct: 121 PYPADQVLRGLEGSFAFVVYDTQTSSVFSALSSDGGESLYWGISGDGSVVMSDDIQIIKQ 180

Query: 181 GCGKSFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSG 240
           GC KSFAPFP GC F +  GL+S++HP N +K +PR+DS G +CGA+FKVDA ++  S  
Sbjct: 181 GCAKSFAPFPNGCMFHSETGLKSFDHPTNMMKAMPRIDSEGVLCGASFKVDACSKINS-- 240

Query: 241 MPRVGSAANWS 248
           +PR GS ANW+
Sbjct: 241 IPRRGSEANWA 248

BLAST of CSPI03G12740 vs. TAIR 10
Match: AT5G19140.1 (Aluminium induced protein with YGL and LRDR motifs )

HSP 1 Score: 214.2 bits (544), Expect = 1.2e-55
Identity = 103/229 (44.98%), Postives = 147/229 (64.19%), Query Frame = 0

Query: 1   MFAVFDKSVAKSPDALQSPESDSTWALKDG-ILAQHFSSVYPGSVIVNLGSSGLLAYSVE 60
           M  +F  ++   P+ L +  S +      G  L   F    P +V V +G    LAYS  
Sbjct: 1   MLGIFSGAIVSPPEELVAAGSRTPSPKTTGSTLVNRFVEKNPSAVSVQVGDYVQLAYSHH 60

Query: 61  KQNPILPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYP 120
            ++P+ PR F   D+IFCLFQG ++N+  LKQQYGL K ANEV++VIEAY+TLRDR PYP
Sbjct: 61  NESPLRPRSFGAKDEIFCLFQGSLDNLGSLKQQYGLAKNANEVLLVIEAYKTLRDRAPYP 120

Query: 121 ADQVVRDIQGKFVFILYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCG 180
           A+ VV  + G F F+++D S+ T F ASD  G VP +WG  ++G +  +DDV+++K  CG
Sbjct: 121 ANHVVAHLSGDFAFVVFDKSTSTLFVASDQVGKVPLYWGITADGYVAFADDVDLLKGACG 180

Query: 181 KSFAPFPKGCFFTTS-GGLRSYEHPLNELKPVPRVDSSGNVCGANFKVD 228
           KS A FP+GC+++T+ GGLRS+E+P N++  VP   + G + GA FKV+
Sbjct: 181 KSLASFPQGCYYSTALGGLRSFENPKNKITAVPA--NEGEIWGATFKVE 227

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P248052.1e-2839.19Stem-specific protein TSJT1 OS=Nicotiana tabacum OX=4097 GN=TSJT1 PE=2 SV=1[more]
P221064.2e-0827.78Asparagine synthetase B [glutamine-hydrolyzing] OS=Escherichia coli (strain K12)... [more]
Q10MX31.8e-0626.32Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Oryza sativa subsp. japonica ... [more]
Q430113.3e-0526.19Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Oryza sativa subsp. japonica ... [more]
Q54MB47.4e-0531.51Probable asparagine synthetase [glutamine-hydrolyzing] OS=Dictyostelium discoide... [more]
Match NameE-valueIdentityDescription
A0A0A0L7D31.5e-141100.00DUF3700 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G145760 PE=... [more]
A0A5D3CZ343.1e-13997.60Stem-specific protein TSJT1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3AXH13.1e-13997.60stem-specific protein TSJT1 OS=Cucumis melo OX=3656 GN=LOC103483669 PE=4 SV=1[more]
A0A6J1IG811.3e-13293.20stem-specific protein TSJT1-like OS=Cucurbita maxima OX=3661 GN=LOC111473103 PE=... [more]
A0A6J1G7B21.3e-13293.20stem-specific protein TSJT1-like OS=Cucurbita moschata OX=3662 GN=LOC111451479 P... [more]
Match NameE-valueIdentityDescription
XP_004134117.13.1e-141100.00stem-specific protein TSJT1 [Cucumis sativus] >KGN56939.1 hypothetical protein C... [more]
XP_008438612.16.4e-13997.60PREDICTED: stem-specific protein TSJT1 [Cucumis melo] >KAA0049327.1 stem-specifi... [more]
XP_038878034.12.4e-13897.20stem-specific protein TSJT1 [Benincasa hispida][more]
XP_022947693.12.6e-13293.20stem-specific protein TSJT1-like [Cucurbita moschata] >XP_022974438.1 stem-speci... [more]
KAG7028873.12.6e-13293.20Stem-specific protein TSJT1, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
Match NameE-valueIdentityDescription
AT3G22850.11.8e-10774.49Aluminium induced protein with YGL and LRDR motifs [more]
AT5G43830.14.2e-10471.20Aluminium induced protein with YGL and LRDR motifs [more]
AT4G27450.16.6e-6549.01Aluminium induced protein with YGL and LRDR motifs [more]
AT3G15450.11.0e-6245.82Aluminium induced protein with YGL and LRDR motifs [more]
AT5G19140.11.2e-5544.98Aluminium induced protein with YGL and LRDR motifs [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024286Domain of unknown function DUF3700SMARTSM01172DUF3700_2coord: 2..226
e-value: 1.1E-149
score: 513.2
IPR024286Domain of unknown function DUF3700PFAMPF12481DUF3700coord: 3..226
e-value: 5.8E-112
score: 372.7
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 56..202
e-value: 3.9E-13
score: 51.1
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 70..199
NoneNo IPR availablePIRSRPIRSR001589-2PIRSR001589-2coord: 77..191
e-value: 3.4E-6
score: 23.7
NoneNo IPR availablePANTHERPTHR45952:SF4ALUMINUM INDUCED PROTEIN WITH YGL AND LRDR MOTIFScoord: 1..250
NoneNo IPR availableCDDcd01910Wali7coord: 3..226
e-value: 1.47552E-140
score: 391.672
IPR044828Stem-specific protein TSJT1-likePANTHERPTHR45952ALUMINUM INDUCED PROTEIN WITH YGL AND LRDR MOTIFScoord: 1..250

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G12740.2CSPI03G12740.2mRNA
CSPI03G12740.1CSPI03G12740.1mRNA