CSPI03G10020 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G10020
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionEnt-kaurenoic acid oxidase 2-like
LocationChr3: 8067482 .. 8072288 (-)
RNA-Seq ExpressionCSPI03G10020
SyntenyCSPI03G10020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATATGGAAGCGATTTCCGGCGGGCCGGGCGGGGCCGATTGGTGGGACAAGGTCCCGTCGAAGTTCCAGGGCTGGGGCAGCGAAGAGTTTGAACGAGTGGATTTATGAGGCCAAATTAGGGGACCGGCGGATGGCTTTGCCGCCTGGGGATTTGGGATGGCCTTTGATTGGAAATATGTTGGGTTTTCTTAGAGCCTTCAAGTCTAACAATCCTGAAACCTTCATTGATAGCTATGTTTCCAGGTCTCCTCTTCTTCTTTTTATAATCATTTCTCTCTCATCTCTCTAGATCTCTTCATTTATTCTCTAAATCATTTCCTCTCTCTAGACATAATTGGGAGCGGATATCTCAACGTGACTGTCCATCAATGCTTTACTGCTACATGCATGGTTGAATGTATTGACCATGGGTGTTCATGGTTCGATTCGAAGCTCAACCGCAAAATACAAAAAAATCTTATACGAAATCGAACCAAACCATACGTTTGTGTCAACCTAAATCGAAACCAAACGCCGGTTCGGTTTAAATCCTAGAAAATTTCACGCCACCAAAGCATTATAAAATTTGCTTTTCTCTTTAAATTTTGGACAATCTTGACCCACTAAAGTACCAAGTTTTACAAAATTTCTCTTTCTATTTTAGATTTTAGTGGATCTCGAGTCAACCTATCTAATGATCTTTATAAACATTTCTTTAATTTTTTTAAAAATAAAGTAGTTCGATTTAATTTTAGAATCGATTTAAAATATTAAACCGAACCGGATAAGAAGAAGAATGTTTTATAAACATTAAACTTTAAATATGATATCTTTTGTCATTTTTTAAAGAAGAAAAAAAAAAGTCCTCTAACTGCTCAAAACTATAGTTTATTTTGGAAATTTTGAAAGTTTTTTTTACATAGCAGTATAAAAAACTATATTTTTTAAGAATATATATTTTTCGAAATACATATAAAGTGATACTTTTTTCCTTTCTCTTTGGATATATTTTTAAAATCTTGTTTGTCCAAAAAAGTTAATAAGAGCCAATATACCTTGGTGTGAGTGTTGCATGCATGATTGATAGACGTTTCATCCTTTTTTTACTTCACAAAAATACCTTTTTTCTCTTTTGATCATGAAAAATTTGAATGTTTGATTTGTAAAAAACAATCTATCGGTTTACCATGCCTAGAGGTTTACCATTATTTAATTCTTCTTTAACTTTACTACATGCATGCATGTCTAGTTGAGTGATATAAAACATATGCTACATGGATTATTGTAAAGTTTACTTGAATCAATGAGTTTTAAAAGTTGTTTTAAGTACTAATTATTTGTTTATTTAGAGAACAACTGAAGTATACTTAGTTTGATTATATCATAGAGAAGTTTAATAATGCTTTATATTTGTGTTTTCTTCTGTCTCATTTCTGACAAGTCACAGAAATATATAATTCAGGTTTTTAAATTACGTAAAGAGAATGAGGGCTATTTTATGGGCTCGAAATTGCAACTTTGTATTTTCATTTCAACACTCTCTTTCTTCCTCACGAAGAATACCTTCTCGGAAATCCTTTCTCTTTTCTTATTCGTTATTTCTTCCTCATTTAAAATAATAAGAAGAGGAAATCAATTTTTATTCCAAAAAGCTTTCTGTATTTTGCATTACAAAATTAGGGAAAGTAATTTAAACCTTAAATTTGGATTTAAAAAATCATTTTCTTTTACCGTTACCGAATAGTTTGTGTTTTTTAAATTTTATTTTAACGAAGAAAAGGACAGGGGAGGGAAAATTACCATAATGACCCTAAAAGGTTTTGAAAGGGCAATGCATGAAAAAGTGTAGAGCACTGTGTTAGTGAAGTAAGCAAACAGCAGACAGACATACAATGAATTTGATTCAAGTTTTTTCTTTTTTCTAAAAAAGTTTATAAATAAAAATTTCCAATATTATTCCCTTTAAAAAAGTTATAAAATTTTTAGAAAATTAATCCAAACAAGAGTTATTCAATCTAGGGAAATTATTCGTTAATTATAATGCTTTTATTGTTATTGTTTTTTAATGGTTTATTGTTACAGTTCTTTTAGTAATTTATAGGGAAATTTCCATGATCATTTGTTTAAGAATATATATTTCTATAAATAAGATATTGATAAAATTGAATTTGTTGGAATAATTTCAATGTCGGTGTGTGGTTTTGCTAATTTTAATTGTTTAGACATTTCTAGTGAAAAAAAAAAAAAAAGTGGAGAATTTGTGTTGTGTTGATCTTATGTTTGCTCAATTCTAGGAGTGCTTTTGTGTCATCATTTTCTAGGTCTCTCTCTTATGAATAAATAAAACCTATAAATTATATATTCATACATCATTTTCAATTATCTATTTGAAACCAAACAATCTCATTTCGTCTATTGTAACATAATTTATGCTGCTTCTTTTTAATATTACTATACATTTTTTAATAGATACATTGGCAAGGACAATGTTGATCATATCTATACAAACTTTATTTTAAAATTAAGGATTTGAGATTTTATGGGTAAGGGTGATAGAAGTATTGAATTTAAAATTTTGTTATAGTTGATAAATATTTAAATTTATTTTAGTATTTTAGAAGGATTGAATTTGAGTTTTGTTTAGAACTATGTATGAATGATGATGAGGAGATTAGAAATCTAATTCTTTTAATTAATTATAGGTTTGGGAAAATCGGAGTGTACAAAATTCATTTGTTTGGAAACCCCAGCGTGGTCGTGACGACGCCGGAGACATGCCGGAAAGTTCTGACCGACGACGAAGCCTTCCAGCCCGGTTGGCCACGAGCAGCTGTAGAGCTCATTGGAAAGAAGTCCTTCATCGAAATGCCGGTCGAGGAACACAAGCGCCTCCGCCGCTTAACCTCTGCTCCGGTGAACGGCTTTGAAGCTTTGAGCAACTACATTCCTTACATTGAAGAGAATGTATTGAAATCCTTGGATAAATGGTCGAATATGGGACCAATCGAATTCTTAACTCAACTTCGTAAGCTCACATTCACCATTATCATGTACATTTTCCTTAGCGCGGAGAGTGAGTCGGTAATGGAATCGTTAGAGAAGGAGTATACTCGGCTTAACTATGGTGTTCGAGCCCTGAGAATTAACATTCCTGGATTTGCTTACCACAAAGCACTCAAGGTGTTTGATAATTTGCCTCAATTAATTACGAAAAGTGTACTTTGGTTTCTGGAATTTGTTTGATCAATTAAACACCATCGGTTGAATGTTTAGGCTCGGAAGAATCTTGTGGCTGCGTTTCAAGGAATTGTGACGGAGCGAAGAAAGAGAAGATTGGGCAATTGGGCGCCGAAGAGGAAGGACATGATGGACGCTCTGATTGATGTTGAAGATGAAAATGGCAGAAAGTTGACTGATGAGGAGATCATTGACATTCTGGTGATGTACTTGAATGCAGGTCATGAATCTTCAGGCCATACCATGATGTGGGCAACTATTTTGTTGAATCAACACCCAGAAGTTCTCAAGAAAGCCAAGGTATATACAAAATTAGGATCTTAATTAGCTTGTTTGGTTACTCAAAATCATGTCATATTTTCATACTTAGTGACAAAATTCTTGTGGTATCATATAAGATCAATCATGATAAAAGTTGGTTTAATAAACTGAACAGGAGGAACAGGAGGAGATTGTGAGAAGGAGGCCTGCAGGTCAAAAGGGATTGACATTGAAAGAATGCAGGGATATGGAATATCTCTCGAAAGTGGTCGATGAAACTCTTCGCTACGTATCATTCTCCCTTGTTGTCTTCAGAGAAGCACAAATGGATGTCAATCTCAATGGTATACCTATCTCTCTCTTCTTTTTTTTTATTGTCGTATTAGCAGTTTACATTTTAACAATGGAGGGTCAAACCATCAACTCTACTGATTAACTATTCTCAAATTGACAATATAGAAATCGCTAGAGTAGTATTTATTGAATGTTTTAGCCTCTCTCTTATTTGAATTGGAACAATGTGGTTGGTCGTTTCATACCATTACATATTTGCGAGAGAGCTCGAAAATTATTCAATAAATCTGCTACAACGTGGCTAGCAATTTTGCACTATTGAATATATGAAAGATGAACTATAAATGTGTTTCCAACTTATACCTAGAGGTTATTAGAAACAGTCAGGTTTTCAGATCCATGAACAATCTTTCCTATGCATCCAAGTGTTAGGAGTGATGGGCACTGAATAATTATTTCCTCTTGTTTATTTCTCCATTGTATGAATGGTGTTTGTGTGATTGCAGGCTATCTAATTCCCAAGGGTTGGAAAGTGCTGGCTTGGTTCAGAAGCATTCACTATGACGATGAAGTTTACCCAGACCCAAAGAAATTTGATCCTTCAAGATGGGATGTATGTTAAAACTCATAGTTTCAATATCCTTTTTACAAAAATTAAAGTTATATCACATCAAATGAAATCCATCTGATATCCACACTGTGTTTGGTATTTGTATAAAGGGATTTATCCCAAAAGCAGGAGAGTTTCTTCCATTTGGAGCAGGAAGCAGGCTGTGCCCAGGAAATGATCTAGCCAAATTAGAGATTTGTATTTTCATTCACTATTTTCTCCTCAATTACAAGTAAGTTCACTTGGAAAAGACAAAATATAATGAGTTTTTTTGTATTAACAATTTGTGTTGTTAATCGGGTTGATTTTTTTCTACAGGCTTGAATGGCTGACCCCTGATTGTCAAATCTTGTATCTGCCCCATTCAAGGCCAAAGGACAATTGCTTGGCTAAAATCAGTAAGAATTCCAGCACTGTTGTGTGA

mRNA sequence

ATGATATGGAAGCGATTTCCGGCGGGCCGGGCGGGGCCGATTGGTGGGACAAGGTCCCGTCGAAGTTCCAGGGCTGGGGCAGCGAAGAGTTTGAACGAGTGGATTTATGAGGCCAAATTAGGGGACCGGCGGATGGCTTTGCCGCCTGGGGATTTGGGATGGCCTTTGATTGGAAATATGTTGGGTTTTCTTAGAGCCTTCAAGTCTAACAATCCTGAAACCTTCATTGATAGCTATGTTTCCAGGTTTGGGAAAATCGGAGTGTACAAAATTCATTTGTTTGGAAACCCCAGCGTGGTCGTGACGACGCCGGAGACATGCCGGAAAGTTCTGACCGACGACGAAGCCTTCCAGCCCGGTTGGCCACGAGCAGCTGTAGAGCTCATTGGAAAGAAGTCCTTCATCGAAATGCCGGTCGAGGAACACAAGCGCCTCCGCCGCTTAACCTCTGCTCCGGTGAACGGCTTTGAAGCTTTGAGCAACTACATTCCTTACATTGAAGAGAATGTATTGAAATCCTTGGATAAATGGTCGAATATGGGACCAATCGAATTCTTAACTCAACTTCGTAAGCTCACATTCACCATTATCATGTACATTTTCCTTAGCGCGGAGAGTGAGTCGGTAATGGAATCGTTAGAGAAGGAGTATACTCGGCTTAACTATGGTGTTCGAGCCCTGAGAATTAACATTCCTGGATTTGCTTACCACAAAGCACTCAAGGCTCGGAAGAATCTTGTGGCTGCGTTTCAAGGAATTGTGACGGAGCGAAGAAAGAGAAGATTGGGCAATTGGGCGCCGAAGAGGAAGGACATGATGGACGCTCTGATTGATGTTGAAGATGAAAATGGCAGAAAGTTGACTGATGAGGAGATCATTGACATTCTGGTGATGTACTTGAATGCAGGTCATGAATCTTCAGGCCATACCATGATGTGGGCAACTATTTTGTTGAATCAACACCCAGAAGTTCTCAAGAAAGCCAAGGAGGAACAGGAGGAGATTGTGAGAAGGAGGCCTGCAGGTCAAAAGGGATTGACATTGAAAGAATGCAGGGATATGGAATATCTCTCGAAAGTGGTCGATGAAACTCTTCGCTACGTATCATTCTCCCTTGTTGTCTTCAGAGAAGCACAAATGGATGTCAATCTCAATGGCTATCTAATTCCCAAGGGTTGGAAAGTGCTGGCTTGGTTCAGAAGCATTCACTATGACGATGAAGTTTACCCAGACCCAAAGAAATTTGATCCTTCAAGATGGGATGGATTTATCCCAAAAGCAGGAGAGTTTCTTCCATTTGGAGCAGGAAGCAGGCTGTGCCCAGGAAATGATCTAGCCAAATTAGAGATTTGTATTTTCATTCACTATTTTCTCCTCAATTACAAGCTTGAATGGCTGACCCCTGATTGTCAAATCTTGTATCTGCCCCATTCAAGGCCAAAGGACAATTGCTTGGCTAAAATCAGTAAGAATTCCAGCACTGTTGTGTGA

Coding sequence (CDS)

ATGATATGGAAGCGATTTCCGGCGGGCCGGGCGGGGCCGATTGGTGGGACAAGGTCCCGTCGAAGTTCCAGGGCTGGGGCAGCGAAGAGTTTGAACGAGTGGATTTATGAGGCCAAATTAGGGGACCGGCGGATGGCTTTGCCGCCTGGGGATTTGGGATGGCCTTTGATTGGAAATATGTTGGGTTTTCTTAGAGCCTTCAAGTCTAACAATCCTGAAACCTTCATTGATAGCTATGTTTCCAGGTTTGGGAAAATCGGAGTGTACAAAATTCATTTGTTTGGAAACCCCAGCGTGGTCGTGACGACGCCGGAGACATGCCGGAAAGTTCTGACCGACGACGAAGCCTTCCAGCCCGGTTGGCCACGAGCAGCTGTAGAGCTCATTGGAAAGAAGTCCTTCATCGAAATGCCGGTCGAGGAACACAAGCGCCTCCGCCGCTTAACCTCTGCTCCGGTGAACGGCTTTGAAGCTTTGAGCAACTACATTCCTTACATTGAAGAGAATGTATTGAAATCCTTGGATAAATGGTCGAATATGGGACCAATCGAATTCTTAACTCAACTTCGTAAGCTCACATTCACCATTATCATGTACATTTTCCTTAGCGCGGAGAGTGAGTCGGTAATGGAATCGTTAGAGAAGGAGTATACTCGGCTTAACTATGGTGTTCGAGCCCTGAGAATTAACATTCCTGGATTTGCTTACCACAAAGCACTCAAGGCTCGGAAGAATCTTGTGGCTGCGTTTCAAGGAATTGTGACGGAGCGAAGAAAGAGAAGATTGGGCAATTGGGCGCCGAAGAGGAAGGACATGATGGACGCTCTGATTGATGTTGAAGATGAAAATGGCAGAAAGTTGACTGATGAGGAGATCATTGACATTCTGGTGATGTACTTGAATGCAGGTCATGAATCTTCAGGCCATACCATGATGTGGGCAACTATTTTGTTGAATCAACACCCAGAAGTTCTCAAGAAAGCCAAGGAGGAACAGGAGGAGATTGTGAGAAGGAGGCCTGCAGGTCAAAAGGGATTGACATTGAAAGAATGCAGGGATATGGAATATCTCTCGAAAGTGGTCGATGAAACTCTTCGCTACGTATCATTCTCCCTTGTTGTCTTCAGAGAAGCACAAATGGATGTCAATCTCAATGGCTATCTAATTCCCAAGGGTTGGAAAGTGCTGGCTTGGTTCAGAAGCATTCACTATGACGATGAAGTTTACCCAGACCCAAAGAAATTTGATCCTTCAAGATGGGATGGATTTATCCCAAAAGCAGGAGAGTTTCTTCCATTTGGAGCAGGAAGCAGGCTGTGCCCAGGAAATGATCTAGCCAAATTAGAGATTTGTATTTTCATTCACTATTTTCTCCTCAATTACAAGCTTGAATGGCTGACCCCTGATTGTCAAATCTTGTATCTGCCCCATTCAAGGCCAAAGGACAATTGCTTGGCTAAAATCAGTAAGAATTCCAGCACTGTTGTGTGA

Protein sequence

MIWKRFPAGRAGPIGGTRSRRSSRAGAAKSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTVV*
Homology
BLAST of CSPI03G10020 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 1.5e-198
Identity = 315/462 (68.18%), Postives = 391/462 (84.63%), Query Frame = 0

Query: 29  KSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKIGV 88
           + +N W+YE+ LG+ R  LPPGDLGWP IGNML FLRAFK+++P++F  + + R+G  G+
Sbjct: 27  RKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPKGI 86

Query: 89  YKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRL 148
           YK H+FGNPS++VTT +TCR+VLTDD+AF+PGWP + +ELIG+KSF+ +  EEHKRLRRL
Sbjct: 87  YKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLRRL 146

Query: 149 TSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAESES 208
           T+APVNG EALS YIPYIEENV+  LDKW+ MG  EFLT LRKLTF IIMYIFLS+ESE+
Sbjct: 147 TAAPVNGHEALSTYIPYIEENVITVLDKWTKMGEFEFLTHLRKLTFRIIMYIFLSSESEN 206

Query: 209 VMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWAPK 268
           VM++LE+EYT LNYGVRA+ +NIPGFAYH+ALKARK LVAAFQ IVTERR +R  N    
Sbjct: 207 VMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQNILSN 266

Query: 269 RKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLKKA 328
           +KDM+D L++V+DE+G+ L DEEIID+L+MYLNAGHESSGHT+MWAT+ L +HPEVL++A
Sbjct: 267 KKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEVLQRA 326

Query: 329 KEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNGYL 388
           K EQE I++ RP GQKGL+LKE R ME+LS+VVDETLR ++FSL  FREA+ DV +NGYL
Sbjct: 327 KAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDVEMNGYL 386

Query: 389 IPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWD-GFIPKAGEFLPFGAGSRLCPGNDLAK 448
           IPKGWKVL WFR +H D EV+PDP+KFDP+RWD GF+PKAG FLPFGAGS LCPGNDLAK
Sbjct: 387 IPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSHLCPGNDLAK 446

Query: 449 LEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKIS 490
           LEI IF+H+FLL Y+++   P+C ++YLPH+RP DNCLA+IS
Sbjct: 447 LEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARIS 488

BLAST of CSPI03G10020 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 684.9 bits (1766), Expect = 6.9e-196
Identity = 316/462 (68.40%), Postives = 389/462 (84.20%), Query Frame = 0

Query: 29  KSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKIGV 88
           K +N WIY +KLG+++  LPPGDLGWP+IGNM  FLRAFK+++PE+FI SY++R+G+ G+
Sbjct: 26  KRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESFIQSYITRYGRTGI 85

Query: 89  YKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRL 148
           YK H+FG P V+VTTPETCR+VLTDD+AF  GWP++ ++LIG+KSF+ +  EEHKRLRRL
Sbjct: 86  YKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKRLRRL 145

Query: 149 TSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAESES 208
           TSAPVNG EALS YI +IEE V   L+KWS MG IEFL+ LRKLTF +IMYIFLS+ESE 
Sbjct: 146 TSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLTFKVIMYIFLSSESEH 205

Query: 209 VMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWAPK 268
           VM+SLE+EYT LNYGVRA+ IN+PGFAYH+ALKARK LVAAFQ IVT RR +R  N +  
Sbjct: 206 VMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRKQNISSN 265

Query: 269 RKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLKKA 328
           RKDM+D LIDV+DENGR L DEEIID+L+MYLNAGHESSGH  MWATIL+ +HP +L+KA
Sbjct: 266 RKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPMILQKA 325

Query: 329 KEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNGYL 388
           KEEQE IV++R  GQK LTLKE R+M YLS+V+DETLR ++FSL  FREA+ DV ++GY+
Sbjct: 326 KEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFREAKSDVQMDGYI 385

Query: 389 IPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKL 448
           IPKGWKVL WFR++H D E+YPDPKKFDPSRW+G+ PKAG FLPFG GS LCPGNDLAKL
Sbjct: 386 IPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHLCPGNDLAKL 445

Query: 449 EICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISK 491
           EI IF+H+FLL Y++E   P C +++LPH+RPKDNCLA+I++
Sbjct: 446 EISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

BLAST of CSPI03G10020 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 1.6e-168
Identity = 278/474 (58.65%), Postives = 360/474 (75.95%), Query Frame = 0

Query: 27  AAKSLNEWIYEAKLG-DRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGK 86
           A ++ + W + A LG  RR  LPPGD+GWPL+G M  FLRAFKS  P++FIDS+  RFG+
Sbjct: 24  AVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFKSGRPDSFIDSFARRFGR 83

Query: 87  IGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRL 146
            G+Y+  +F +P+++ TTPE C++VL DD+AF  GWP+A V LIG KSF+ M  +EH+RL
Sbjct: 84  AGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVALIGPKSFVNMGYDEHRRL 143

Query: 147 RRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNM---GPIEFLTQLRKLTFTIIMYIFL 206
           R+LT+AP+NGF+AL++Y+ +I++ V+ +L  WS     G  EFLT+LR++TF II+ IF+
Sbjct: 144 RKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLTELRRMTFRIIVQIFM 203

Query: 207 SAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKR-- 266
               E     LE+ YT LNYG+RA+ I++PGFAYHKA++AR+ LVAA Q ++ ERR R  
Sbjct: 204 GGADERTAAELERTYTELNYGMRAMAIDLPGFAYHKAIRARRRLVAALQRVLDERRARGG 263

Query: 267 RLGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQ 326
           +    A    DMMD LI VEDE GR+L D+EIID+LVMYLNAGHESSGH  MWAT+ L +
Sbjct: 264 KTAAGAAAPVDMMDRLIAVEDEGGRRLQDDEIIDVLVMYLNAGHESSGHITMWATVFLQE 323

Query: 327 HPEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQM 386
           +PE+L KAK EQE I+R  P GQKGLTL++ R M YLS+VVDETLR+V+ S V FR+A  
Sbjct: 324 NPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYLSQVVDETLRFVNISFVSFRQATR 383

Query: 387 DVNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLC 446
           DV +NGYLIPKGWKV  W+RS+H D +VYPDPKKFDPSRW+G  P+AG FLPFG G+RLC
Sbjct: 384 DVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDPSRWEGPPPRAGTFLPFGLGTRLC 443

Query: 447 PGNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSST 495
           PGNDLAKLEI +F+H+FLL YKL    P+C++ YLPH RP DNCLAKI++ SS+
Sbjct: 444 PGNDLAKLEISVFLHHFLLGYKLTRKNPNCRVRYLPHPRPVDNCLAKITRLSSS 497

BLAST of CSPI03G10020 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 8.2e-165
Identity = 269/469 (57.36%), Postives = 352/469 (75.05%), Query Frame = 0

Query: 27  AAKSLNEWIYEAKLG-DRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGK 86
           AA+  + W  EA LG  RR  LPPG++GWPL+G M  FLRAFKS  P+ FI S+V RFG+
Sbjct: 51  AARRAHGWYREAALGAARRARLPPGEMGWPLVGGMWAFLRAFKSGKPDAFIASFVRRFGR 110

Query: 87  IGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRL 146
            GVY+  +F +P+V+VTT E C++VL DD+AF  GWP+A V L+G +SF+ MP +EH+R+
Sbjct: 111 TGVYRSFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRSFVAMPYDEHRRI 170

Query: 147 RRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNM-GPIEFLTQLRKLTFTIIMYIFLSA 206
           R+LT+AP+NGF+AL+ Y+P+I+  V  SL  W++  G +EFLT+LR++TF II+ IFL  
Sbjct: 171 RKLTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKIIVQIFLGG 230

Query: 207 ESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGN 266
             ++   +LE+ YT LNYG+RA+ IN+PGFAY  AL+AR+ LVA  QG++ ERR  R   
Sbjct: 231 ADQATTRALERSYTELNYGMRAMAINLPGFAYRGALRARRRLVAVLQGVLDERRAARAKG 290

Query: 267 WAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEV 326
            +    DMMD LI+ +DE GR L D+EIID+LVMYLNAGHESSGH  MWAT+ L ++P++
Sbjct: 291 VSGGGVDMMDRLIEAQDERGRHLDDDEIIDVLVMYLNAGHESSGHITMWATVFLQENPDM 350

Query: 327 LKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNL 386
             +AK EQE I+R  P+ Q+GLTL++ R MEYLS+V+DETLR V+ S V FR+A  DV +
Sbjct: 351 FARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDETLRLVNISFVSFRQATRDVFV 410

Query: 387 NGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGND 446
           NGYLIPKGWKV  W+RS+H D +VYPDP KFDPSRW+G  P+AG FL FG G+RLCPGND
Sbjct: 411 NGYLIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGHSPRAGTFLAFGLGARLCPGND 470

Query: 447 LAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSS 494
           LAKLEI +F+H+FLL YKL    P C++ YLPH RP DNCLAKI++  S
Sbjct: 471 LAKLEISVFLHHFLLGYKLARTNPRCRVRYLPHPRPVDNCLAKITRVGS 519

BLAST of CSPI03G10020 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 8.2e-165
Identity = 263/465 (56.56%), Postives = 357/465 (76.77%), Query Frame = 0

Query: 32  NEWIYEAKLG-DRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKIGVYK 91
           ++W+  A LG +RR  LPPG++GWP++G+M  FLRAFKS NP+ FI S++ RFG+ GVY+
Sbjct: 39  HDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFKSGNPDAFIASFIRRFGRTGVYR 98

Query: 92  IHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRLTS 151
             +F +P+++  TPE C++VL DDE F  GWP+A V LIG KSF+ M  ++H+R+R+LT+
Sbjct: 99  TFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLIGPKSFVNMSYDDHRRIRKLTA 158

Query: 152 APVNGFEALSNYIPYIEENVLKSLDKWSN--MGPIEFLTQLRKLTFTIIMYIFLSAESES 211
           AP+NGF+AL+ Y+ +I++ V+ SL +WS+   G +EFLT+LR++TF II+ IF+S   ++
Sbjct: 159 APINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTELRRMTFKIIVQIFMSGADDA 218

Query: 212 VMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRL-GNWAP 271
            ME+LE+ YT LNYG+RA+ IN+PGFAY++AL+AR+ LV+  QG++  RR     G    
Sbjct: 219 TMEALERSYTDLNYGMRAMAINLPGFAYYRALRARRKLVSVLQGVLDGRRAAAAKGFKRS 278

Query: 272 KRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLKK 331
              DMMD LI+ EDE GR+L D+EI+D+L+MYLNAGHESSGH  MWAT+ L ++P++  +
Sbjct: 279 GAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAGHESSGHITMWATVFLQENPDIFAR 338

Query: 332 AKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNGY 391
           AK EQEEI+R  PA Q GLTL++ + M +LS+VVDETLR V+ S V FR+A  D+ +NGY
Sbjct: 339 AKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVDETLRCVNISFVSFRQATRDIFVNGY 398

Query: 392 LIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAK 451
           LIPKGWKV  W+RS+H DD+VYPDPK F+PSRW+G  PKAG FLPFG G+RLCPGNDLAK
Sbjct: 399 LIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWEGPPPKAGTFLPFGLGARLCPGNDLAK 458

Query: 452 LEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNS 493
           LEI +F+H+FLL YKL+   P C++ YLPH RP DNCLA I+K S
Sbjct: 459 LEISVFLHHFLLGYKLKRANPKCRVRYLPHPRPVDNCLATITKVS 503

BLAST of CSPI03G10020 vs. ExPASy TrEMBL
Match: A0A5D3CZZ0 (Ent-kaurenoic acid oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003480 PE=3 SV=1)

HSP 1 Score: 948.7 bits (2451), Expect = 9.5e-273
Identity = 459/470 (97.66%), Postives = 463/470 (98.51%), Query Frame = 0

Query: 27  AAKSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKI 86
           AAKS NEWIYEAKLG+RRMALPPGDLGWPLIGNMLGFLRAFKS NPETFIDSYVSRFGKI
Sbjct: 29  AAKSFNEWIYEAKLGERRMALPPGDLGWPLIGNMLGFLRAFKSKNPETFIDSYVSRFGKI 88

Query: 87  GVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR 146
           GVYK+HLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR
Sbjct: 89  GVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR 148

Query: 147 RLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAES 206
           RLTSAPVNGFEALSNYIPYIEENVLKSLDKWS MGPIEFLTQLRKLTFTIIMYIFLSAES
Sbjct: 149 RLTSAPVNGFEALSNYIPYIEENVLKSLDKWSTMGPIEFLTQLRKLTFTIIMYIFLSAES 208

Query: 207 ESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWA 266
           ESVMESLEKEYTRLNYGVRALRIN+PGFAYHKALKARKNLVAA QGIVTERRKRRLG WA
Sbjct: 209 ESVMESLEKEYTRLNYGVRALRINLPGFAYHKALKARKNLVAALQGIVTERRKRRLGKWA 268

Query: 267 PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK 326
           PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK
Sbjct: 269 PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK 328

Query: 327 KAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG 386
           KAKEEQEEIVRRRP GQKGLTLKECR+MEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG
Sbjct: 329 KAKEEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG 388

Query: 387 YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA 446
           YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA
Sbjct: 389 YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA 448

Query: 447 KLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTVV 497
           KLEICIFIHYFLLNYKLEWLTPDC ILYLPHSRPKDNCLAKISKNSSTVV
Sbjct: 449 KLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKISKNSSTVV 498

BLAST of CSPI03G10020 vs. ExPASy TrEMBL
Match: A0A1S3AWI4 (ent-kaurenoic acid oxidase 2-like OS=Cucumis melo OX=3656 GN=LOC103483403 PE=3 SV=1)

HSP 1 Score: 947.2 bits (2447), Expect = 2.8e-272
Identity = 458/469 (97.65%), Postives = 462/469 (98.51%), Query Frame = 0

Query: 27  AAKSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKI 86
           AAKS NEWIYEAKLG+RRMALPPGDLGWPLIGNMLGFLRAFKS NPETFIDSYVSRFGKI
Sbjct: 29  AAKSFNEWIYEAKLGERRMALPPGDLGWPLIGNMLGFLRAFKSKNPETFIDSYVSRFGKI 88

Query: 87  GVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR 146
           GVYK+HLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR
Sbjct: 89  GVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR 148

Query: 147 RLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAES 206
           RLTSAPVNGFEALSNYIPYIEENVLKSLDKWS MGPIEFLTQLRKLTFTIIMYIFLSAES
Sbjct: 149 RLTSAPVNGFEALSNYIPYIEENVLKSLDKWSTMGPIEFLTQLRKLTFTIIMYIFLSAES 208

Query: 207 ESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWA 266
           ESVMESLEKEYTRLNYGVRALRIN+PGFAYHKALKARKNLVAA QGIVTERRKRRLG WA
Sbjct: 209 ESVMESLEKEYTRLNYGVRALRINLPGFAYHKALKARKNLVAALQGIVTERRKRRLGKWA 268

Query: 267 PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK 326
           PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK
Sbjct: 269 PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK 328

Query: 327 KAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG 386
           KAKEEQEEIVRRRP GQKGLTLKECR+MEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG
Sbjct: 329 KAKEEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG 388

Query: 387 YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA 446
           YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA
Sbjct: 389 YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA 448

Query: 447 KLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTV 496
           KLEICIFIHYFLLNYKLEWLTPDC ILYLPHSRPKDNCLAKISKNSSTV
Sbjct: 449 KLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKISKNSSTV 497

BLAST of CSPI03G10020 vs. ExPASy TrEMBL
Match: A0A5A7U100 (Ent-kaurenoic acid oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G003090 PE=3 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 4.4e-262
Identity = 442/452 (97.79%), Postives = 445/452 (98.45%), Query Frame = 0

Query: 45  MALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKIGVYKIHLFGNPSVVVTTP 104
           MALPPGDLGWPLIGNMLGFLRAFKS NPETFIDSYVSRFGKIGVYK+HLFGNPSVVVTTP
Sbjct: 1   MALPPGDLGWPLIGNMLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTP 60

Query: 105 ETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIP 164
           ETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIP
Sbjct: 61  ETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIP 120

Query: 165 YIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGV 224
           YIEENVLKSLDKWS MGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGV
Sbjct: 121 YIEENVLKSLDKWSTMGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGV 180

Query: 225 RALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWAPKRKDMMDALIDVEDENG 284
           RALRIN+PGFAYHKALKARKNLVAA QGIVTERRKRRLG WAPKRKDMMDALIDVEDENG
Sbjct: 181 RALRINLPGFAYHKALKARKNLVAALQGIVTERRKRRLGKWAPKRKDMMDALIDVEDENG 240

Query: 285 RKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRPAGQK 344
           RKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRP GQK
Sbjct: 241 RKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRPPGQK 300

Query: 345 GLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHY 404
           GLTLKECR+MEYLSKVVDETLRYVSFSLV FREAQMDVNLNGYLIPKGWKVLAWFRSIHY
Sbjct: 301 GLTLKECREMEYLSKVVDETLRYVSFSLVAFREAQMDVNLNGYLIPKGWKVLAWFRSIHY 360

Query: 405 DDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLE 464
           DDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLE
Sbjct: 361 DDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLE 420

Query: 465 WLTPDCQILYLPHSRPKDNCLAKISKNSSTVV 497
           WLTPDC ILYLPHSRPKDNCLAKISKNSSTVV
Sbjct: 421 WLTPDCPILYLPHSRPKDNCLAKISKNSSTVV 452

BLAST of CSPI03G10020 vs. ExPASy TrEMBL
Match: A0A6J1E9S4 (ent-kaurenoic acid oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432076 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 8.6e-258
Identity = 424/467 (90.79%), Postives = 449/467 (96.15%), Query Frame = 0

Query: 27  AAKSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKI 86
           AAKSLNEWIYEAKLGDRR+ALPPGDLGWPLIGNMLGFLRAFKS NPETFID YVSR+GK 
Sbjct: 27  AAKSLNEWIYEAKLGDRRLALPPGDLGWPLIGNMLGFLRAFKSKNPETFIDGYVSRYGKT 86

Query: 87  GVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR 146
           GVYK+HLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAA+ELIG+KSFI+MP EEHKRLR
Sbjct: 87  GVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAIELIGEKSFIQMPEEEHKRLR 146

Query: 147 RLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAES 206
           RLTSAPVNGFEALSNYIPYIE+NVL+SL+KWS MGPIEFLTQLRKLTFT+IMYIFLS+ES
Sbjct: 147 RLTSAPVNGFEALSNYIPYIEKNVLESLEKWSKMGPIEFLTQLRKLTFTVIMYIFLSSES 206

Query: 207 ESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWA 266
           E VME LEKEYTRLNYGVRA+RIN+PGFAYHKALKARKNLVAA +GIVTERR+++L  WA
Sbjct: 207 EPVMEMLEKEYTRLNYGVRAMRINLPGFAYHKALKARKNLVAALKGIVTERRRQKLDKWA 266

Query: 267 PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK 326
           PKRKDMMD LID+EDENGRKL DEEIIDIL+MYLNAGHESSGHTMMWATILLNQHPEVLK
Sbjct: 267 PKRKDMMDQLIDIEDENGRKLDDEEIIDILIMYLNAGHESSGHTMMWATILLNQHPEVLK 326

Query: 327 KAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG 386
           KA+EEQE IVR RPAGQ GLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG
Sbjct: 327 KAREEQEAIVRNRPAGQTGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG 386

Query: 387 YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA 446
           YLIPKGWKVLAWFRSIHYD EVYPDPKKFDPSRWDGF+PKAGEFLPFGAGSRLCPGNDLA
Sbjct: 387 YLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRWDGFVPKAGEFLPFGAGSRLCPGNDLA 446

Query: 447 KLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSS 494
           KLEICIF+HYFLLNY LEWLTPDC+ILYLPHSRPKDNC+AKI+K SS
Sbjct: 447 KLEICIFVHYFLLNYNLEWLTPDCEILYLPHSRPKDNCMAKITKKSS 493

BLAST of CSPI03G10020 vs. ExPASy TrEMBL
Match: A0A6J1IVT9 (ent-kaurenoic acid oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479695 PE=3 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 1.2e-256
Identity = 423/467 (90.58%), Postives = 448/467 (95.93%), Query Frame = 0

Query: 27  AAKSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKI 86
           AAKSLNEWIYEAKLGDRR+ALPPGDLGWPLIGNMLGFLRAFKS NPETFID YVSR+GK 
Sbjct: 27  AAKSLNEWIYEAKLGDRRLALPPGDLGWPLIGNMLGFLRAFKSKNPETFIDGYVSRYGKT 86

Query: 87  GVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR 146
           GVYK+HLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIG+KSFI+MP EEHKRLR
Sbjct: 87  GVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGEKSFIQMPQEEHKRLR 146

Query: 147 RLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAES 206
           RLTSAPVNGFEALSNYIPYIE+NVL+SL+KWS MGPIEFLTQLRKLTFT+IMYIFLS+ES
Sbjct: 147 RLTSAPVNGFEALSNYIPYIEKNVLESLEKWSKMGPIEFLTQLRKLTFTVIMYIFLSSES 206

Query: 207 ESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWA 266
           E VME LEKEYTRLNYGVRA+RIN+PGFAYHKALKARKNLVAA +GIVTERR+++L  WA
Sbjct: 207 EPVMEMLEKEYTRLNYGVRAMRINLPGFAYHKALKARKNLVAALKGIVTERRRQKLDKWA 266

Query: 267 PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK 326
           PKRKDMMD LID+ DENGRKL DEEIIDIL+MYLNAGHESSGHTMMWATILLNQHPEVLK
Sbjct: 267 PKRKDMMDQLIDIVDENGRKLDDEEIIDILIMYLNAGHESSGHTMMWATILLNQHPEVLK 326

Query: 327 KAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG 386
           KA+EEQE IVR RPAGQ GLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG
Sbjct: 327 KAREEQEAIVRNRPAGQTGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG 386

Query: 387 YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA 446
           YLIPKGWKVLAWFRSIHYD EVYPDPKKF+PSRWDGF+PKAGEFLPFGAGSRLCPGNDLA
Sbjct: 387 YLIPKGWKVLAWFRSIHYDSEVYPDPKKFEPSRWDGFVPKAGEFLPFGAGSRLCPGNDLA 446

Query: 447 KLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSS 494
           KLEICIF+HYFLLNY LEWLTPDC+ILYLPHSRPKDNC+AKI+K SS
Sbjct: 447 KLEICIFVHYFLLNYNLEWLTPDCEILYLPHSRPKDNCMAKITKKSS 493

BLAST of CSPI03G10020 vs. NCBI nr
Match: XP_004133946.1 (ent-kaurenoic acid oxidase 1 isoform X1 [Cucumis sativus] >KAE8650281.1 hypothetical protein Csa_010193 [Cucumis sativus])

HSP 1 Score: 968.0 bits (2501), Expect = 3.1e-278
Identity = 470/470 (100.00%), Postives = 470/470 (100.00%), Query Frame = 0

Query: 27  AAKSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKI 86
           AAKSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKI
Sbjct: 29  AAKSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKI 88

Query: 87  GVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR 146
           GVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR
Sbjct: 89  GVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR 148

Query: 147 RLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAES 206
           RLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAES
Sbjct: 149 RLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAES 208

Query: 207 ESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWA 266
           ESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWA
Sbjct: 209 ESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWA 268

Query: 267 PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK 326
           PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK
Sbjct: 269 PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK 328

Query: 327 KAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG 386
           KAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG
Sbjct: 329 KAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG 388

Query: 387 YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA 446
           YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA
Sbjct: 389 YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA 448

Query: 447 KLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTVV 497
           KLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTVV
Sbjct: 449 KLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTVV 498

BLAST of CSPI03G10020 vs. NCBI nr
Match: TYK17483.1 (ent-kaurenoic acid oxidase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 948.7 bits (2451), Expect = 2.0e-272
Identity = 459/470 (97.66%), Postives = 463/470 (98.51%), Query Frame = 0

Query: 27  AAKSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKI 86
           AAKS NEWIYEAKLG+RRMALPPGDLGWPLIGNMLGFLRAFKS NPETFIDSYVSRFGKI
Sbjct: 29  AAKSFNEWIYEAKLGERRMALPPGDLGWPLIGNMLGFLRAFKSKNPETFIDSYVSRFGKI 88

Query: 87  GVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR 146
           GVYK+HLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR
Sbjct: 89  GVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR 148

Query: 147 RLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAES 206
           RLTSAPVNGFEALSNYIPYIEENVLKSLDKWS MGPIEFLTQLRKLTFTIIMYIFLSAES
Sbjct: 149 RLTSAPVNGFEALSNYIPYIEENVLKSLDKWSTMGPIEFLTQLRKLTFTIIMYIFLSAES 208

Query: 207 ESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWA 266
           ESVMESLEKEYTRLNYGVRALRIN+PGFAYHKALKARKNLVAA QGIVTERRKRRLG WA
Sbjct: 209 ESVMESLEKEYTRLNYGVRALRINLPGFAYHKALKARKNLVAALQGIVTERRKRRLGKWA 268

Query: 267 PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK 326
           PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK
Sbjct: 269 PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK 328

Query: 327 KAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG 386
           KAKEEQEEIVRRRP GQKGLTLKECR+MEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG
Sbjct: 329 KAKEEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG 388

Query: 387 YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA 446
           YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA
Sbjct: 389 YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA 448

Query: 447 KLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTVV 497
           KLEICIFIHYFLLNYKLEWLTPDC ILYLPHSRPKDNCLAKISKNSSTVV
Sbjct: 449 KLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKISKNSSTVV 498

BLAST of CSPI03G10020 vs. NCBI nr
Match: XP_008438234.1 (PREDICTED: ent-kaurenoic acid oxidase 2-like [Cucumis melo])

HSP 1 Score: 947.2 bits (2447), Expect = 5.7e-272
Identity = 458/469 (97.65%), Postives = 462/469 (98.51%), Query Frame = 0

Query: 27  AAKSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKI 86
           AAKS NEWIYEAKLG+RRMALPPGDLGWPLIGNMLGFLRAFKS NPETFIDSYVSRFGKI
Sbjct: 29  AAKSFNEWIYEAKLGERRMALPPGDLGWPLIGNMLGFLRAFKSKNPETFIDSYVSRFGKI 88

Query: 87  GVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR 146
           GVYK+HLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR
Sbjct: 89  GVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR 148

Query: 147 RLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAES 206
           RLTSAPVNGFEALSNYIPYIEENVLKSLDKWS MGPIEFLTQLRKLTFTIIMYIFLSAES
Sbjct: 149 RLTSAPVNGFEALSNYIPYIEENVLKSLDKWSTMGPIEFLTQLRKLTFTIIMYIFLSAES 208

Query: 207 ESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWA 266
           ESVMESLEKEYTRLNYGVRALRIN+PGFAYHKALKARKNLVAA QGIVTERRKRRLG WA
Sbjct: 209 ESVMESLEKEYTRLNYGVRALRINLPGFAYHKALKARKNLVAALQGIVTERRKRRLGKWA 268

Query: 267 PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK 326
           PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK
Sbjct: 269 PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK 328

Query: 327 KAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG 386
           KAKEEQEEIVRRRP GQKGLTLKECR+MEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG
Sbjct: 329 KAKEEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG 388

Query: 387 YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA 446
           YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA
Sbjct: 389 YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA 448

Query: 447 KLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTV 496
           KLEICIFIHYFLLNYKLEWLTPDC ILYLPHSRPKDNCLAKISKNSSTV
Sbjct: 449 KLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKISKNSSTV 497

BLAST of CSPI03G10020 vs. NCBI nr
Match: XP_038902869.1 (ent-kaurenoic acid oxidase 1-like [Benincasa hispida])

HSP 1 Score: 925.6 bits (2391), Expect = 1.8e-265
Identity = 447/470 (95.11%), Postives = 456/470 (97.02%), Query Frame = 0

Query: 27  AAKSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKI 86
           AAKSLNEWIYEAKLGDRRMALPPGDLGWPLIGNML FLRAFKS NPETFIDSYVSRFGKI
Sbjct: 23  AAKSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLAFLRAFKSKNPETFIDSYVSRFGKI 82

Query: 87  GVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLR 146
           GVYK+HLFGNPSVVVTTPETCRKVLTDDEAFQPGWPR+A+ELIGKKSFIEMPVEEHKRLR
Sbjct: 83  GVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRSAIELIGKKSFIEMPVEEHKRLR 142

Query: 147 RLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAES 206
           RLTSAPVNGFEALSNYIPYIEE+VLKSL KWS+MGPIEFLTQLRKLTFTIIMYIFLSAES
Sbjct: 143 RLTSAPVNGFEALSNYIPYIEESVLKSLGKWSDMGPIEFLTQLRKLTFTIIMYIFLSAES 202

Query: 207 ESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWA 266
           E VME+LEKEYTRLNYGVRALRIN PGFAYHKALKARKNLVAA Q IVT+RRKRRL  WA
Sbjct: 203 EPVMEALEKEYTRLNYGVRALRINFPGFAYHKALKARKNLVAALQRIVTDRRKRRLDKWA 262

Query: 267 PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK 326
           PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK
Sbjct: 263 PKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLK 322

Query: 327 KAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG 386
           KAK EQEEIVRRRP GQKGLTLKECR+MEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG
Sbjct: 323 KAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNG 382

Query: 387 YLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLA 446
           YLIPKGWKVLAWFRSIHYD EVYPDPKKFDPSRWD F+PKAGEFLPFGAGSRLCPGNDLA
Sbjct: 383 YLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRWDNFVPKAGEFLPFGAGSRLCPGNDLA 442

Query: 447 KLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTVV 497
           KLEICIFIHYFLLNYKLEWLTPDC ILYLPHSRPKDNCLAKISKNSSTVV
Sbjct: 443 KLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKISKNSSTVV 492

BLAST of CSPI03G10020 vs. NCBI nr
Match: KAA0049080.1 (ent-kaurenoic acid oxidase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 913.3 bits (2359), Expect = 9.1e-262
Identity = 442/452 (97.79%), Postives = 445/452 (98.45%), Query Frame = 0

Query: 45  MALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKIGVYKIHLFGNPSVVVTTP 104
           MALPPGDLGWPLIGNMLGFLRAFKS NPETFIDSYVSRFGKIGVYK+HLFGNPSVVVTTP
Sbjct: 1   MALPPGDLGWPLIGNMLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTP 60

Query: 105 ETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIP 164
           ETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIP
Sbjct: 61  ETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIP 120

Query: 165 YIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGV 224
           YIEENVLKSLDKWS MGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGV
Sbjct: 121 YIEENVLKSLDKWSTMGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGV 180

Query: 225 RALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWAPKRKDMMDALIDVEDENG 284
           RALRIN+PGFAYHKALKARKNLVAA QGIVTERRKRRLG WAPKRKDMMDALIDVEDENG
Sbjct: 181 RALRINLPGFAYHKALKARKNLVAALQGIVTERRKRRLGKWAPKRKDMMDALIDVEDENG 240

Query: 285 RKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRPAGQK 344
           RKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRP GQK
Sbjct: 241 RKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRPPGQK 300

Query: 345 GLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHY 404
           GLTLKECR+MEYLSKVVDETLRYVSFSLV FREAQMDVNLNGYLIPKGWKVLAWFRSIHY
Sbjct: 301 GLTLKECREMEYLSKVVDETLRYVSFSLVAFREAQMDVNLNGYLIPKGWKVLAWFRSIHY 360

Query: 405 DDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLE 464
           DDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLE
Sbjct: 361 DDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLE 420

Query: 465 WLTPDCQILYLPHSRPKDNCLAKISKNSSTVV 497
           WLTPDC ILYLPHSRPKDNCLAKISKNSSTVV
Sbjct: 421 WLTPDCPILYLPHSRPKDNCLAKISKNSSTVV 452

BLAST of CSPI03G10020 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 693.7 bits (1789), Expect = 1.1e-199
Identity = 315/462 (68.18%), Postives = 391/462 (84.63%), Query Frame = 0

Query: 29  KSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKIGV 88
           + +N W+YE+ LG+ R  LPPGDLGWP IGNML FLRAFK+++P++F  + + R+G  G+
Sbjct: 27  RKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPKGI 86

Query: 89  YKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRL 148
           YK H+FGNPS++VTT +TCR+VLTDD+AF+PGWP + +ELIG+KSF+ +  EEHKRLRRL
Sbjct: 87  YKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLRRL 146

Query: 149 TSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAESES 208
           T+APVNG EALS YIPYIEENV+  LDKW+ MG  EFLT LRKLTF IIMYIFLS+ESE+
Sbjct: 147 TAAPVNGHEALSTYIPYIEENVITVLDKWTKMGEFEFLTHLRKLTFRIIMYIFLSSESEN 206

Query: 209 VMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWAPK 268
           VM++LE+EYT LNYGVRA+ +NIPGFAYH+ALKARK LVAAFQ IVTERR +R  N    
Sbjct: 207 VMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQNILSN 266

Query: 269 RKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLKKA 328
           +KDM+D L++V+DE+G+ L DEEIID+L+MYLNAGHESSGHT+MWAT+ L +HPEVL++A
Sbjct: 267 KKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEVLQRA 326

Query: 329 KEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNGYL 388
           K EQE I++ RP GQKGL+LKE R ME+LS+VVDETLR ++FSL  FREA+ DV +NGYL
Sbjct: 327 KAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDVEMNGYL 386

Query: 389 IPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWD-GFIPKAGEFLPFGAGSRLCPGNDLAK 448
           IPKGWKVL WFR +H D EV+PDP+KFDP+RWD GF+PKAG FLPFGAGS LCPGNDLAK
Sbjct: 387 IPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSHLCPGNDLAK 446

Query: 449 LEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKIS 490
           LEI IF+H+FLL Y+++   P+C ++YLPH+RP DNCLA+IS
Sbjct: 447 LEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARIS 488

BLAST of CSPI03G10020 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 684.9 bits (1766), Expect = 4.9e-197
Identity = 316/462 (68.40%), Postives = 389/462 (84.20%), Query Frame = 0

Query: 29  KSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKIGV 88
           K +N WIY +KLG+++  LPPGDLGWP+IGNM  FLRAFK+++PE+FI SY++R+G+ G+
Sbjct: 26  KRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESFIQSYITRYGRTGI 85

Query: 89  YKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRL 148
           YK H+FG P V+VTTPETCR+VLTDD+AF  GWP++ ++LIG+KSF+ +  EEHKRLRRL
Sbjct: 86  YKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKRLRRL 145

Query: 149 TSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAESES 208
           TSAPVNG EALS YI +IEE V   L+KWS MG IEFL+ LRKLTF +IMYIFLS+ESE 
Sbjct: 146 TSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLTFKVIMYIFLSSESEH 205

Query: 209 VMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWAPK 268
           VM+SLE+EYT LNYGVRA+ IN+PGFAYH+ALKARK LVAAFQ IVT RR +R  N +  
Sbjct: 206 VMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRKQNISSN 265

Query: 269 RKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLKKA 328
           RKDM+D LIDV+DENGR L DEEIID+L+MYLNAGHESSGH  MWATIL+ +HP +L+KA
Sbjct: 266 RKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPMILQKA 325

Query: 329 KEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNGYL 388
           KEEQE IV++R  GQK LTLKE R+M YLS+V+DETLR ++FSL  FREA+ DV ++GY+
Sbjct: 326 KEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFREAKSDVQMDGYI 385

Query: 389 IPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKL 448
           IPKGWKVL WFR++H D E+YPDPKKFDPSRW+G+ PKAG FLPFG GS LCPGNDLAKL
Sbjct: 386 IPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHLCPGNDLAKL 445

Query: 449 EICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISK 491
           EI IF+H+FLL Y++E   P C +++LPH+RPKDNCLA+I++
Sbjct: 446 EISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

BLAST of CSPI03G10020 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 684.9 bits (1766), Expect = 4.9e-197
Identity = 316/462 (68.40%), Postives = 389/462 (84.20%), Query Frame = 0

Query: 29  KSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKIGV 88
           K +N WIY +KLG+++  LPPGDLGWP+IGNM  FLRAFK+++PE+FI SY++R+G+ G+
Sbjct: 26  KRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESFIQSYITRYGRTGI 85

Query: 89  YKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRL 148
           YK H+FG P V+VTTPETCR+VLTDD+AF  GWP++ ++LIG+KSF+ +  EEHKRLRRL
Sbjct: 86  YKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKRLRRL 145

Query: 149 TSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAESES 208
           TSAPVNG EALS YI +IEE V   L+KWS MG IEFL+ LRKLTF +IMYIFLS+ESE 
Sbjct: 146 TSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLTFKVIMYIFLSSESEH 205

Query: 209 VMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWAPK 268
           VM+SLE+EYT LNYGVRA+ IN+PGFAYH+ALKARK LVAAFQ IVT RR +R  N +  
Sbjct: 206 VMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRKQNISSN 265

Query: 269 RKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLKKA 328
           RKDM+D LIDV+DENGR L DEEIID+L+MYLNAGHESSGH  MWATIL+ +HP +L+KA
Sbjct: 266 RKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPMILQKA 325

Query: 329 KEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNGYL 388
           KEEQE IV++R  GQK LTLKE R+M YLS+V+DETLR ++FSL  FREA+ DV ++GY+
Sbjct: 326 KEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFREAKSDVQMDGYI 385

Query: 389 IPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKL 448
           IPKGWKVL WFR++H D E+YPDPKKFDPSRW+G+ PKAG FLPFG GS LCPGNDLAKL
Sbjct: 386 IPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHLCPGNDLAKL 445

Query: 449 EICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISK 491
           EI IF+H+FLL Y++E   P C +++LPH+RPKDNCLA+I++
Sbjct: 446 EISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

BLAST of CSPI03G10020 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 277.7 bits (709), Expect = 1.8e-74
Identity = 162/460 (35.22%), Postives = 249/460 (54.13%), Query Frame = 0

Query: 35  IYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKIGVYKIHLF 94
           I   K  + R  LPPG +GWP +G  L       S NP  F  S   R+G+I  +K  + 
Sbjct: 22  IVSKKKKNSRGKLPPGSMGWPYLGETL----QLYSQNPNVFFTSKQKRYGEI--FKTRIL 81

Query: 95  GNPSVVVTTPETCRKVL-TDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPV 154
           G P V++ +PE  R VL T    F+P +PR+  +LIG  +      + H  +R+L  +  
Sbjct: 82  GYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHIRKLVQSSF 141

Query: 155 NGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTI-IMYIFLSAESESVMES 214
              E +   IP IE   L SL  W+NM  +    +++K  F + I+ IF   ES S  E 
Sbjct: 142 YP-ETIRKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILAIFGHLES-SYKEI 201

Query: 215 LEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWAPKRKDM 274
           L+  Y  ++ G  +  +++PG +YHKAL ARK L      I+ ERR++R       + D 
Sbjct: 202 LKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKR-----ALQTDF 261

Query: 275 MDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLKKAKEEQ 334
           +  L++ ++E GR LT E+I D ++  L A  +++   + W    L+   ++L+  K EQ
Sbjct: 262 LGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVKAEQ 321

Query: 335 EEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNGYLIPKG 394
           + I       +K LT ++ R+M    KV+ E+LR  S     FREA +DV   GYLIPKG
Sbjct: 322 KAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYLIPKG 381

Query: 395 WKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICI 454
           WKV+  FR+IH++ + + +P+ FDPSR++   PK   F+PFG+G   CPGN+LAKL+I I
Sbjct: 382 WKVMPLFRNIHHNPKYFSNPEVFDPSRFE-VNPKPNTFMPFGSGVHACPGNELAKLQILI 441

Query: 455 FIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNS 493
           F+H+ + N++ E    +  I Y P   P++   A   ++S
Sbjct: 442 FLHHLVSNFRWEVKGGEKGIQYSPFPIPQNGLPATFRRHS 467

BLAST of CSPI03G10020 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 261.2 bits (666), Expect = 1.7e-69
Identity = 152/454 (33.48%), Postives = 238/454 (52.42%), Query Frame = 0

Query: 35  IYEAKLGDRRMALPPGDLGWPLIGNMLGFLRAFKSNNPETFIDSYVSRFGKIGVYKIHLF 94
           I + + G  ++ LPPG +GWP +G          S +P  F  S   R+G   V+K H+ 
Sbjct: 24  ISQRRFGSSKLPLPPGTMGWPYVGETF----QLYSQDPNVFFQSKQKRYG--SVFKTHVL 83

Query: 95  GNPSVVVTTPETCRKVL-TDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPV 154
           G P V++++PE  + VL T    F+P +P +   ++GK++      + H +LR+L    +
Sbjct: 84  GCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLV---L 143

Query: 155 NGF--EALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAESESVME 214
             F  E++ N +P IE     SL  W     I    +++  TF + +      +     E
Sbjct: 144 RAFMPESIRNMVPDIESIAQDSLRSWEGT-MINTYQEMKTYTFNVALLSIFGKDEVLYRE 203

Query: 215 SLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWAPKRKD 274
            L++ Y  L  G  ++ +N+PG  +HK++KARK L      I++ERR+           D
Sbjct: 204 DLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQN-----GSSHND 263

Query: 275 MMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLKKAKEE 334
           ++ + +  ++E    LTDE+I D ++  + A  +++   M W    L ++P VL+   EE
Sbjct: 264 LLGSFMGDKEE----LTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEE 323

Query: 335 QEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNGYLIPK 394
           Q  I + +  G+  LT  + + M   S+V+ ETLR  S     FREA  DV   GYLIPK
Sbjct: 324 QMAIRKDKEEGE-SLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPK 383

Query: 395 GWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEIC 454
           GWKVL  FR+IH+  +++ +P KFDPSR++   PK   F+PFG G+  CPGN+LAKLE+ 
Sbjct: 384 GWKVLPLFRNIHHSADIFSNPGKFDPSRFE-VAPKPNTFMPFGNGTHSCPGNELAKLEMS 443

Query: 455 IFIHYFLLNYKLEWLTPDCQILYLPHSR-PKDNC 485
           I IH+    Y+   L        + HSR PK +C
Sbjct: 444 IMIHHLTTKYRFGQLLERATGFSMGHSRFPKTDC 456

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O230511.5e-19868.18Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1[more]
Q9C5Y26.9e-19668.40Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2[more]
Q9AXH91.6e-16858.65Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1[more]
Q432468.2e-16557.36Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1[more]
Q5VRM78.2e-16556.56Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A5D3CZZ09.5e-27397.66Ent-kaurenoic acid oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3AWI42.8e-27297.65ent-kaurenoic acid oxidase 2-like OS=Cucumis melo OX=3656 GN=LOC103483403 PE=3 S... [more]
A0A5A7U1004.4e-26297.79Ent-kaurenoic acid oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A6J1E9S48.6e-25890.79ent-kaurenoic acid oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432076 ... [more]
A0A6J1IVT91.2e-25690.58ent-kaurenoic acid oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479695 PE... [more]
Match NameE-valueIdentityDescription
XP_004133946.13.1e-278100.00ent-kaurenoic acid oxidase 1 isoform X1 [Cucumis sativus] >KAE8650281.1 hypothet... [more]
TYK17483.12.0e-27297.66ent-kaurenoic acid oxidase 2-like [Cucumis melo var. makuwa][more]
XP_008438234.15.7e-27297.65PREDICTED: ent-kaurenoic acid oxidase 2-like [Cucumis melo][more]
XP_038902869.11.8e-26595.11ent-kaurenoic acid oxidase 1-like [Benincasa hispida][more]
KAA0049080.19.1e-26297.79ent-kaurenoic acid oxidase 2-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G05160.11.1e-19968.18cytochrome P450, family 88, subfamily A, polypeptide 3 [more]
AT2G32440.14.9e-19768.40ent-kaurenoic acid hydroxylase 2 [more]
AT2G32440.24.9e-19768.40ent-kaurenoic acid hydroxylase 2 [more]
AT3G19270.11.8e-7435.22cytochrome P450, family 707, subfamily A, polypeptide 4 [more]
AT4G19230.21.7e-6933.48cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 430..440
score: 57.74
coord: 440..463
score: 29.77
coord: 398..422
score: 26.17
coord: 311..337
score: 28.82
IPR001128Cytochrome P450PRINTSPR00385P450coord: 359..370
score: 33.11
coord: 302..319
score: 27.64
coord: 440..451
score: 38.98
coord: 431..440
score: 59.89
IPR001128Cytochrome P450PFAMPF00067p450coord: 48..465
e-value: 1.1E-68
score: 232.2
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 38..491
e-value: 5.9E-109
score: 366.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 43..488
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 28..484
NoneNo IPR availablePANTHERPTHR24286:SF289ENT-KAURENOIC ACID OXIDASE 2coord: 28..484
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 433..442

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G10020.1CSPI03G10020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0051777 ent-kaurenoate oxidase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen