CSPI03G05060 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G05060
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	DUF4228 domain protein
Location	Chr3: 4099600 .. 4100203 (+)
RNA-Seq Expression	CSPI03G05060
Synteny	CSPI03G05060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATCTCTCCTTCTCCAAATCTCACCTTCCTCTCTCTCTCTCTCTCTCCAGAGAAATTTAGAGCAATTAGAAGCGTTCCCCATGGGAAACTGTGTGAGCAGCAATTGTTTATGTGGAAAACCAACAAGAATACTTAGCGATGAGGAAGCTCTAGAGATGATGAAGAGAAGAATTATGGCTTACGATGAGACTCCGAGATCTTCGAAGGCGGCAACAACAACAGAGGTGAAGATCAAGATCACAAAAAAAGAACTAGAGATGTTACTAGGTAATTATACGGTGGTGGAGGTGAAGGACGAGCTGCCCTCGGCACATTTCTTTTCGCAGCCAATCGTCGAATTGCAGCCAATCGACATCGACGATGCCTTCGAGATGCAGCCACGATCATGGCGGCCTTCCCTGCAGAGTATCCCTGAGATGGATTGAAATCCAGAGTGATTTTATCTTCTTTTTCTTTTCTTCTTCTTACTCATCTTGTTATGGTGTGGTTTTCCATGAACACTTTTGGGGATCTCTGTTTTCTCTCTCTGGTTTAATATTTGGAGTTTTTTGGTCTCCAAATTGTTTCTCTGAGACACATATAGAACCAAACCAATAGTAAACTT

mRNA sequence

CATCTCTCCTTCTCCAAATCTCACCTTCCTCTCTCTCTCTCTCTCTCCAGAGAAATTTAGAGCAATTAGAAGCGTTCCCCATGGGAAACTGTGTGAGCAGCAATTGTTTATGTGGAAAACCAACAAGAATACTTAGCGATGAGGAAGCTCTAGAGATGATGAAGAGAAGAATTATGGCTTACGATGAGACTCCGAGATCTTCGAAGGCGGCAACAACAACAGAGGTGAAGATCAAGATCACAAAAAAAGAACTAGAGATGTTACTAGGTAATTATACGGTGGTGGAGGTGAAGGACGAGCTGCCCTCGGCACATTTCTTTTCGCAGCCAATCGTCGAATTGCAGCCAATCGACATCGACGATGCCTTCGAGATGCAGCCACGATCATGGCGGCCTTCCCTGCAGAGTATCCCTGAGATGGATTGAAATCCAGAGTGATTTTATCTTCTTTTTCTTTTCTTCTTCTTACTCATCTTGTTATGGTGTGGTTTTCCATGAACACTTTTGGGGATCTCTGTTTTCTCTCTCTGGTTTAATATTTGGAGTTTTTTGGTCTCCAAATTGTTTCTCTGAGACACATATAGAACCAAACCAATAGTAAACTT

Coding sequence (CDS)

ATGGGAAACTGTGTGAGCAGCAATTGTTTATGTGGAAAACCAACAAGAATACTTAGCGATGAGGAAGCTCTAGAGATGATGAAGAGAAGAATTATGGCTTACGATGAGACTCCGAGATCTTCGAAGGCGGCAACAACAACAGAGGTGAAGATCAAGATCACAAAAAAAGAACTAGAGATGTTACTAGGTAATTATACGGTGGTGGAGGTGAAGGACGAGCTGCCCTCGGCACATTTCTTTTCGCAGCCAATCGTCGAATTGCAGCCAATCGACATCGACGATGCCTTCGAGATGCAGCCACGATCATGGCGGCCTTCCCTGCAGAGTATCCCTGAGATGGATTGA

Protein sequence

MGNCVSSNCLCGKPTRILSDEEALEMMKRRIMAYDETPRSSKAATTTEVKIKITKKELEMLLGNYTVVEVKDELPSAHFFSQPIVELQPIDIDDAFEMQPRSWRPSLQSIPEMD*

Homology

[Close]

BLAST of CSPI03G05060 vs. ExPASy TrEMBL
Match: A0A0A0L7M3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G076570 PE=4 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 6.8e-57
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MGNCVSSNCLCGKPTRILSDEEALEMMKRRIMAYDETPRSSKAATTTEVKIKITKKELEM 60
           MGNCVSSNCLCGKPTRILSDEEALEMMKRRIMAYDETPRSSKAATTTEVKIKITKKELEM
Sbjct: 1   MGNCVSSNCLCGKPTRILSDEEALEMMKRRIMAYDETPRSSKAATTTEVKIKITKKELEM 60

Query: 61  LLGNYTVVEVKDELPSAHFFSQPIVELQPIDIDDAFEMQPRSWRPSLQSIPEMD 115
           LLGNYTVVEVKDELPSAHFFSQPIVELQPIDIDDAFEMQPRSWRPSLQSIPEMD
Sbjct: 61  LLGNYTVVEVKDELPSAHFFSQPIVELQPIDIDDAFEMQPRSWRPSLQSIPEMD 114

[Close]

BLAST of CSPI03G05060 vs. ExPASy TrEMBL
Match: A0A5D3BTP7 (DUF4228 domain protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold925G00060 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.2e-32
Identity = 87/124 (70.16%), Postives = 93/124 (75.00%), Query Frame = 0


Query: 1   MGNCVSSNCLCGKPTRILSDEEALEMMKRRIMAY-------DETPRSSKAA---TTTEVK 60
           MGNCVSS  LCGK TRI SDEEALEMMKR +MAY       DETPRSS AA   TTTEVK
Sbjct: 1   MGNCVSSKYLCGKSTRI-SDEEALEMMKRTVMAYNDETPKIDETPRSSVAATTTTTTEVK 60

Query: 61  IKITKKELEMLLGNYTVVEVKDELPSAHFFSQPIVELQPIDIDDAFEMQPRSWRPSLQSI 115
           IKITK+ELEMLLGNY VVEVK+EL +  F S      + IDID+  EMQPRSWRPSLQSI
Sbjct: 61  IKITKRELEMLLGNYKVVEVKEELSTTQFLS------RLIDIDEGIEMQPRSWRPSLQSI 117

[Close]

BLAST of CSPI03G05060 vs. ExPASy TrEMBL
Match: A0A1S4E465 (uncharacterized protein LOC103501581 OS=Cucumis melo OX=3656 GN=LOC103501581 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.2e-32
Identity = 87/124 (70.16%), Postives = 93/124 (75.00%), Query Frame = 0


Query: 1   MGNCVSSNCLCGKPTRILSDEEALEMMKRRIMAY-------DETPRSSKAA---TTTEVK 60
           MGNCVSS  LCGK TRI SDEEALEMMKR +MAY       DETPRSS AA   TTTEVK
Sbjct: 1   MGNCVSSKYLCGKSTRI-SDEEALEMMKRTVMAYNDETPKIDETPRSSVAATTTTTTEVK 60

Query: 61  IKITKKELEMLLGNYTVVEVKDELPSAHFFSQPIVELQPIDIDDAFEMQPRSWRPSLQSI 115
           IKITK+ELEMLLGNY VVEVK+EL +  F S      + IDID+  EMQPRSWRPSLQSI
Sbjct: 61  IKITKRELEMLLGNYKVVEVKEELSTTQFLS------RLIDIDEGIEMQPRSWRPSLQSI 117

[Close]

BLAST of CSPI03G05060 vs. ExPASy TrEMBL
Match: A0A5D3BTQ9 (DUF4228 domain protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold925G00050 PE=4 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 4.3e-11
Identity = 51/115 (44.35%), Postives = 68/115 (59.13%), Query Frame = 0


Query: 1   MGNCVSSNCLCGKPTRILSDEEALEMMKRRIMAYDETPRSSKAATTTEVKIKITKKELEM 60
           MGNC S+  L  +   +  DEE +E M RR +  +   +   A+TTTE+KI+ITKKELE 
Sbjct: 1   MGNCTSNKYL-AETMPMSDDEEEVEEMLRRRLVVESRAQKCSASTTTELKIRITKKELEK 60

Query: 61  LLGNYTVVEVKDELPSAHFFSQPIVELQPIDIDDAFE-MQPRSWRPSLQSIPEMD 115
           LL    +    +ELP A   S      + ID+ DAFE    RSW+PSLQSIPE++
Sbjct: 61  LLCKVDM----EELPVAELLS------KLIDVGDAFESTHRRSWQPSLQSIPEVN 104

[Close]

BLAST of CSPI03G05060 vs. ExPASy TrEMBL
Match: A0A1S3CJ77 (uncharacterized protein LOC103501580 OS=Cucumis melo OX=3656 GN=LOC103501580 PE=4 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 4.3e-11
Identity = 51/115 (44.35%), Postives = 68/115 (59.13%), Query Frame = 0


Query: 1   MGNCVSSNCLCGKPTRILSDEEALEMMKRRIMAYDETPRSSKAATTTEVKIKITKKELEM 60
           MGNC S+  L  +   +  DEE +E M RR +  +   +   A+TTTE+KI+ITKKELE 
Sbjct: 1   MGNCTSNKYL-AETMPMSDDEEEVEEMLRRRLVVESRAQKCSASTTTELKIRITKKELEK 60

Query: 61  LLGNYTVVEVKDELPSAHFFSQPIVELQPIDIDDAFE-MQPRSWRPSLQSIPEMD 115
           LL    +    +ELP A   S      + ID+ DAFE    RSW+PSLQSIPE++
Sbjct: 61  LLCKVDM----EELPVAELLS------KLIDVGDAFESTHRRSWQPSLQSIPEVN 104

[Close]

BLAST of CSPI03G05060 vs. NCBI nr
Match: XP_011652736.1 (uncharacterized protein LOC105435072 [Cucumis sativus] >KGN56121.1 hypothetical protein Csa_011128 [Cucumis sativus])

HSP 1 Score: 229.6 bits (584), Expect = 1.4e-56
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MGNCVSSNCLCGKPTRILSDEEALEMMKRRIMAYDETPRSSKAATTTEVKIKITKKELEM 60
           MGNCVSSNCLCGKPTRILSDEEALEMMKRRIMAYDETPRSSKAATTTEVKIKITKKELEM
Sbjct: 1   MGNCVSSNCLCGKPTRILSDEEALEMMKRRIMAYDETPRSSKAATTTEVKIKITKKELEM 60

Query: 61  LLGNYTVVEVKDELPSAHFFSQPIVELQPIDIDDAFEMQPRSWRPSLQSIPEMD 115
           LLGNYTVVEVKDELPSAHFFSQPIVELQPIDIDDAFEMQPRSWRPSLQSIPEMD
Sbjct: 61  LLGNYTVVEVKDELPSAHFFSQPIVELQPIDIDDAFEMQPRSWRPSLQSIPEMD 114

[Close]

BLAST of CSPI03G05060 vs. NCBI nr
Match: XP_016903026.1 (PREDICTED: uncharacterized protein LOC103501581 [Cucumis melo] >KAA0033990.1 DUF4228 domain protein [Cucumis melo var. makuwa] >TYK02555.1 DUF4228 domain protein [Cucumis melo var. makuwa])

HSP 1 Score: 149.1 bits (375), Expect = 2.4e-32
Identity = 87/124 (70.16%), Postives = 93/124 (75.00%), Query Frame = 0


Query: 1   MGNCVSSNCLCGKPTRILSDEEALEMMKRRIMAY-------DETPRSSKAA---TTTEVK 60
           MGNCVSS  LCGK TRI SDEEALEMMKR +MAY       DETPRSS AA   TTTEVK
Sbjct: 1   MGNCVSSKYLCGKSTRI-SDEEALEMMKRTVMAYNDETPKIDETPRSSVAATTTTTTEVK 60

Query: 61  IKITKKELEMLLGNYTVVEVKDELPSAHFFSQPIVELQPIDIDDAFEMQPRSWRPSLQSI 115
           IKITK+ELEMLLGNY VVEVK+EL +  F S      + IDID+  EMQPRSWRPSLQSI
Sbjct: 61  IKITKRELEMLLGNYKVVEVKEELSTTQFLS------RLIDIDEGIEMQPRSWRPSLQSI 117

[Close]

BLAST of CSPI03G05060 vs. NCBI nr
Match: KAG6600784.1 (hypothetical protein SDJN03_06017, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031421.1 hypothetical protein SDJN02_05461, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 134.4 bits (337), Expect = 6.1e-28
Identity = 73/114 (64.04%), Postives = 85/114 (74.56%), Query Frame = 0


Query: 1   MGNCVSSNCLCGKPTRILSDEEALEMMKRRIMAYDETPRSSKAATTTEVKIKITKKELEM 60
           MGNCVS+   CGK +RI SDEEALEM+KR +MAYDE P +S  ATTTE+KIKITK+ELEM
Sbjct: 1   MGNCVSNKIFCGKSSRI-SDEEALEMVKRSLMAYDEAPVASVTATTTELKIKITKRELEM 60

Query: 61  LLGNYTVVEVKDELPSAHFFSQPIVELQPIDIDDAFEMQPRSWRPSLQSIPEMD 115
           LL NY VVEVK+EL +    S P+       +D   EMQPRSW+PSLQSIPE D
Sbjct: 61  LLANYNVVEVKEELQTEQLLS-PL-----FYVDGTLEMQPRSWQPSLQSIPEAD 107

[Close]

BLAST of CSPI03G05060 vs. NCBI nr
Match: XP_038904231.1 (uncharacterized protein LOC120090574 [Benincasa hispida])

HSP 1 Score: 83.6 bits (205), Expect = 1.2e-12
Identity = 54/115 (46.96%), Postives = 68/115 (59.13%), Query Frame = 0


Query: 1   MGNCVSSNCLCGKPTRILSDEEALEMMKRRIMAYDETPRSSKAATTTEVKIKITKKELEM 60
           MGNC S+  L         DEEA+E ++RR++ +D   R   A TTTE+KI+ITK+ELE 
Sbjct: 1   MGNCTSNKYLAETMPMSDEDEEAVETVRRRLVVHDGA-RKCSANTTTELKIRITKRELEK 60

Query: 61  LLGNYTVVEVKDELPSAHFFSQPIVELQPIDIDDAFE-MQPRSWRPSLQSIPEMD 115
           LL    V    +ELP     S      Q ID+ DAFE    RSWRPSLQSIPE++
Sbjct: 61  LLSKVDV----EELPVTELLS------QLIDVGDAFESTHQRSWRPSLQSIPEVN 104

[Close]

BLAST of CSPI03G05060 vs. NCBI nr
Match: XP_008463409.1 (PREDICTED: uncharacterized protein LOC103501580 [Cucumis melo] >TYK02554.1 DUF4228 domain protein [Cucumis melo var. makuwa])

HSP 1 Score: 77.4 bits (189), Expect = 8.9e-11
Identity = 51/115 (44.35%), Postives = 68/115 (59.13%), Query Frame = 0


Query: 1   MGNCVSSNCLCGKPTRILSDEEALEMMKRRIMAYDETPRSSKAATTTEVKIKITKKELEM 60
           MGNC S+  L  +   +  DEE +E M RR +  +   +   A+TTTE+KI+ITKKELE 
Sbjct: 1   MGNCTSNKYL-AETMPMSDDEEEVEEMLRRRLVVESRAQKCSASTTTELKIRITKKELEK 60

Query: 61  LLGNYTVVEVKDELPSAHFFSQPIVELQPIDIDDAFE-MQPRSWRPSLQSIPEMD 115
           LL    +    +ELP A   S      + ID+ DAFE    RSW+PSLQSIPE++
Sbjct: 61  LLCKVDM----EELPVAELLS------KLIDVGDAFESTHRRSWQPSLQSIPEVN 104

[Close]

BLAST of CSPI03G05060 vs. TAIR 10
Match: AT4G21920.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G20340.1); Has 40 Blast hits to 40 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 40; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 44.3 bits (103), Expect = 7.8e-05
Identity = 42/136 (30.88%), Postives = 62/136 (45.59%), Query Frame = 0


Query: 1   MGNCVSSNCLCGKPTRILSDEEALEMMKRR-----IMAYDE---------------TPRS 60
           MGNC+   C+  K T   S ++     KRR      + +D+               +  S
Sbjct: 1   MGNCI---CVTEKTTTSWSGDDNGSYNKRRRRRRSTVVHDDNDDGEKLLGETSNVTSTSS 60

Query: 61  SKAATTTEVKIKITKKELEMLLGNYTVVEVKDE--LPSAHFFSQPIVELQPIDIDDAFEM 115
           S ++   E+KI+ITKKELE L+ N  +  +  E  L    F     +    +D+ +    
Sbjct: 61  SSSSERREIKIRITKKELEDLMRNIGLKSLTAEEILSKLIFEGGDQIGFSAVDVTN--HH 120

The following BLAST results are available for this feature:

BLAST of CSPI03G05060 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI03G05060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L7M3	6.8e-57	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G076570 PE=4 SV=1	[more]
A0A5D3BTP7	1.2e-32	70.16	DUF4228 domain protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold9...	[more]
A0A1S4E465	1.2e-32	70.16	uncharacterized protein LOC103501581 OS=Cucumis melo OX=3656 GN=LOC103501581 PE=...	[more]
A0A5D3BTQ9	4.3e-11	44.35	DUF4228 domain protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold9...	[more]
A0A1S3CJ77	4.3e-11	44.35	uncharacterized protein LOC103501580 OS=Cucumis melo OX=3656 GN=LOC103501580 PE=...	[more]

BLAST of CSPI03G05060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011652736.1	1.4e-56	100.00	uncharacterized protein LOC105435072 [Cucumis sativus] >KGN56121.1 hypothetical ...	[more]
XP_016903026.1	2.4e-32	70.16	PREDICTED: uncharacterized protein LOC103501581 [Cucumis melo] >KAA0033990.1 DUF...	[more]
KAG6600784.1	6.1e-28	64.04	hypothetical protein SDJN03_06017, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038904231.1	1.2e-12	46.96	uncharacterized protein LOC120090574 [Benincasa hispida]	[more]
XP_008463409.1	8.9e-11	44.35	PREDICTED: uncharacterized protein LOC103501580 [Cucumis melo] >TYK02554.1 DUF42...	[more]

BLAST of CSPI03G05060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21920.1	7.8e-05	30.88	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33647:SF5	OS01G0793900 PROTEIN	coord: 1..113
None	No IPR available	PANTHER	PTHR33647	OS01G0793900 PROTEIN	coord: 1..113

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G05060.1	CSPI03G05060.1	mRNA