CSPI03G04480 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G04480
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionpirin-like protein
LocationChr3: 3645544 .. 3647020 (-)
RNA-Seq ExpressionCSPI03G04480
SyntenyCSPI03G04480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCTCAAGCAATCTCCCTCTCAGTCTTTTCCCTTTGACCTCTCCCAAATTCAAATTTTTCCTATTTTTTCTCTCTCTATAAATACCTTAGAAATCCCCAAGACAGAAAGATTAAGAAAAGTTTAATTGATGATGGCTCTACACAGGAATCATTTAGGTTCAATAATAAAAGAGCCTCGTTTTGTAGTTAAGAAGTTTCTTGCTAGACCTCAACATGAAGGCCTTGGAGCTGTTGTTAGAAGAAGCATCGGCAGGTAATTCTTTTATTCTTTCCTTTTTAATCCCATTTTTCTTTCTTTTTTTTTTATTTTTATTTTGATTTTTATTTTTCAGGTTTGAGCTTAAATACTTTGATCCATTCCTTGTTCTTGATGAGTTTTCTGGTAAACCCCCAGTTCTACAAATCTCTTTCAATTTTTTAAGTTTTTAGTTATTTAATGTTGTGTTATGGAAATTAAATCGAAATATTTTGCTTATTATTGTAGTGAGTGCACCTGGTGGGTTTCCTGATCATCCACATAGAGGATTTGAAACTGTCACTTACATGTTACAGGTTTCTTTTTTTCCTTTTCTAAATTATTTTCAAGCTCTTTATCGATTTCACTTACATCTTCAAGGTTTATTTATCTAGAGTAGGTTGTGCACAGAGAAATTAAATGGAAAATCATTTATTTATGTTTACTTCTATAATGAATGAGTTTTGTTTTATGTTGAAAATGATGAAAAGGGAGCAATGATACATGAAGATTTTGAAGGGCATAAAGGAAGAATTGAAGTTGGTGACTTGCAATGGATGACTGCTGGCAAAGGCATTGTTCATTCTGAAATCCCTTCTTCCATTGGCACCCAACGTGGCTTACAACTTTGGATCAATCTTTCCTCCAAACACAAAATGTAAGTCGCTGTCCTTTTATCAATTTTCAAAAAAATTATACATCAATTTATCTTCTGAAATAATCTTTAAAAACAAAAATTTGAATGAAAGGATTGAACCGAGGTATCAAGAAATACACAACGAGAACATAGTGGAAGCTACAAGAGAAGGAGTAAAAGTGAGAGTGATAGCGGGTGAAGCCTTGGGAGTAAAATCCCCAATCTACACCAAAACTCCAACCATGTACTTGGACTTCACTCTCGTCCCGGGATCTCGCATTGAGCAGCCAATCCCGACGGGGTGGAATGCGTTCGTGTACGTGCTAGAAGGCGATGGCGGAGTATTCGGAAGCATGAAATTGATGCCGATGGTCACACCACATCACCTTCTTTTGTTAGGGAATGGGGATGGGTTGGAGGTTTGGAACAAGTCATCCACCAAAACTCTAAGGTTCATATTGGTTGGAGGTGAGCCATTGAATGAGAGTGTTGTGCAGTTGGGGCCATTTGTGATGAACACTCAAGAAGAGATTGACCAAACCGTTGAGGATTTTGAGAATTGTACAAATGGCTTTGAGAGAGCAAGGCATTGGAAGAGTTCTTAA

mRNA sequence

TCCTCAAGCAATCTCCCTCTCAGTCTTTTCCCTTTGACCTCTCCCAAATTCAAATTTTTCCTATTTTTTCTCTCTCTATAAATACCTTAGAAATCCCCAAGACAGAAAGATTAAGAAAAGTTTAATTGATGATGGCTCTACACAGGAATCATTTAGGTTCAATAATAAAAGAGCCTCGTTTTGTAGTTAAGAAGTTTCTTGCTAGACCTCAACATGAAGGCCTTGGAGCTGTTGTTAGAAGAAGCATCGGCAGGTTTGAGCTTAAATACTTTGATCCATTCCTTGTTCTTGATGAGTTTTCTGTGAGTGCACCTGGTGGGTTTCCTGATCATCCACATAGAGGATTTGAAACTGTCACTTACATGTTACAGGGAGCAATGATACATGAAGATTTTGAAGGGCATAAAGGAAGAATTGAAGTTGGTGACTTGCAATGGATGACTGCTGGCAAAGGCATTGTTCATTCTGAAATCCCTTCTTCCATTGGCACCCAACGTGGCTTACAACTTTGGATCAATCTTTCCTCCAAACACAAAATGATTGAACCGAGGTATCAAGAAATACACAACGAGAACATAGTGGAAGCTACAAGAGAAGGAGTAAAAGTGAGAGTGATAGCGGGTGAAGCCTTGGGAGTAAAATCCCCAATCTACACCAAAACTCCAACCATGTACTTGGACTTCACTCTCGTCCCGGGATCTCGCATTGAGCAGCCAATCCCGACGGGGTGGAATGCGTTCGTGTACGTGCTAGAAGGCGATGGCGGAGTATTCGGAAGCATGAAATTGATGCCGATGGTCACACCACATCACCTTCTTTTGTTAGGGAATGGGGATGGGTTGGAGGTTTGGAACAAGTCATCCACCAAAACTCTAAGGTTCATATTGGTTGGAGGTGAGCCATTGAATGAGAGTGTTGTGCAGTTGGGGCCATTTGTGATGAACACTCAAGAAGAGATTGACCAAACCGTTGAGGATTTTGAGAATTGTACAAATGGCTTTGAGAGAGCAAGGCATTGGAAGAGTTCTTAA

Coding sequence (CDS)

ATGATGGCTCTACACAGGAATCATTTAGGTTCAATAATAAAAGAGCCTCGTTTTGTAGTTAAGAAGTTTCTTGCTAGACCTCAACATGAAGGCCTTGGAGCTGTTGTTAGAAGAAGCATCGGCAGGTTTGAGCTTAAATACTTTGATCCATTCCTTGTTCTTGATGAGTTTTCTGTGAGTGCACCTGGTGGGTTTCCTGATCATCCACATAGAGGATTTGAAACTGTCACTTACATGTTACAGGGAGCAATGATACATGAAGATTTTGAAGGGCATAAAGGAAGAATTGAAGTTGGTGACTTGCAATGGATGACTGCTGGCAAAGGCATTGTTCATTCTGAAATCCCTTCTTCCATTGGCACCCAACGTGGCTTACAACTTTGGATCAATCTTTCCTCCAAACACAAAATGATTGAACCGAGGTATCAAGAAATACACAACGAGAACATAGTGGAAGCTACAAGAGAAGGAGTAAAAGTGAGAGTGATAGCGGGTGAAGCCTTGGGAGTAAAATCCCCAATCTACACCAAAACTCCAACCATGTACTTGGACTTCACTCTCGTCCCGGGATCTCGCATTGAGCAGCCAATCCCGACGGGGTGGAATGCGTTCGTGTACGTGCTAGAAGGCGATGGCGGAGTATTCGGAAGCATGAAATTGATGCCGATGGTCACACCACATCACCTTCTTTTGTTAGGGAATGGGGATGGGTTGGAGGTTTGGAACAAGTCATCCACCAAAACTCTAAGGTTCATATTGGTTGGAGGTGAGCCATTGAATGAGAGTGTTGTGCAGTTGGGGCCATTTGTGATGAACACTCAAGAAGAGATTGACCAAACCGTTGAGGATTTTGAGAATTGTACAAATGGCTTTGAGAGAGCAAGGCATTGGAAGAGTTCTTAA

Protein sequence

MMALHRNHLGSIIKEPRFVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSAPGGFPDHPHRGFETVTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIGTQRGLQLWINLSSKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPTMYLDFTLVPGSRIEQPIPTGWNAFVYVLEGDGGVFGSMKLMPMVTPHHLLLLGNGDGLEVWNKSSTKTLRFILVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKSS*
Homology
BLAST of CSPI03G04480 vs. ExPASy Swiss-Prot
Match: Q9LPS9 (Pirin-like protein At1g50590 OS=Arabidopsis thaliana OX=3702 GN=At1g50590 PE=2 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 9.9e-113
Identity = 204/290 (70.34%), Postives = 228/290 (78.62%), Query Frame = 0

Query: 17  RFVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSAPGGFPDHPHRGFETV 76
           R VVKK  AR  HEG GAVVRRSIGRFE +YFDPFLVLDEFSVSAP GFPDHPHRGFETV
Sbjct: 13  RLVVKKLFARQLHEGFGAVVRRSIGRFEFRYFDPFLVLDEFSVSAPAGFPDHPHRGFETV 72

Query: 77  TYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSS----IGTQRGLQLWINLS 136
           TYML+G ++HED EGHKG I  G LQWMTAGKGIVHSE+PSS    I   +GLQLWINLS
Sbjct: 73  TYMLEGEILHEDCEGHKGVIREGGLQWMTAGKGIVHSEMPSSNSNGITHNKGLQLWINLS 132

Query: 137 SKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPTMYLDFTLVPGSR 196
           S+ K++EP YQEI +++I E  ++GV+VRVIAGE  GVKS I T+TPTMYLDFTL PGSR
Sbjct: 133 SRQKLVEPSYQEIESKDIAETEKDGVRVRVIAGEWNGVKSKICTRTPTMYLDFTLSPGSR 192

Query: 197 IEQPIPTGWNAFVYVLEGDGGVFGSMKLM-PMVTPHHLLLLG-NGDGLEVWNKS-STKTL 256
           I QPIP  WNAFVYVL+G G  FG  KL       HHLL+LG  GD LE WN S S   L
Sbjct: 193 ISQPIPLHWNAFVYVLQGHGH-FGDSKLQHSAAAAHHLLVLGLGGDMLEAWNGSDSGLPL 252

Query: 257 RFILVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKS 300
           RFILV GEP+ E +VQ GPFVMNTQEEID+T++DFEN  NGFE+ARHWKS
Sbjct: 253 RFILVAGEPIGEPMVQFGPFVMNTQEEIDETIDDFENFRNGFEKARHWKS 301

BLAST of CSPI03G04480 vs. ExPASy Swiss-Prot
Match: Q9SEE4 (Pirin-like protein OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 2.7e-110
Identity = 191/292 (65.41%), Postives = 231/292 (79.11%), Query Frame = 0

Query: 9   LGSIIKEPRFVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSAPGGFPDH 68
           + SI   PR VVKK LAR Q+EG GA+VRRSIGR EL+  DPFL+LDEFSVS P GFPDH
Sbjct: 3   MSSIFSRPRLVVKKVLARAQNEGDGAIVRRSIGRPELQNLDPFLMLDEFSVSQPAGFPDH 62

Query: 69  PHRGFETVTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIGTQRGLQLW 128
           PHRGFETVTYMLQGA  H+DF GHKG I  GD+QWMTAG+GIVHSE+P+  GTQ+GLQLW
Sbjct: 63  PHRGFETVTYMLQGAFTHQDFAGHKGTIRTGDVQWMTAGRGIVHSEMPAGPGTQKGLQLW 122

Query: 129 INLSSKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPTMYLDFTLV 188
           INLSSK KMIEPRYQE+ +++I +A ++GV V ++AGE++G KS ++T+TPTMYLDFTL 
Sbjct: 123 INLSSKDKMIEPRYQELLHQDIPKAEKDGVSVTILAGESMGKKSQVFTRTPTMYLDFTLK 182

Query: 189 PGSRIEQPIPTGWNAFVYVLEGDGGVFGSMKLMPMVTP-HHLLLLGNGDGLEVWNKSSTK 248
           PGS   QPIP  WNAF+Y++EG+ G FGS       TP HH L+LG G+GL VWNKSS K
Sbjct: 183 PGSEHHQPIPETWNAFLYIVEGE-GAFGSSD--STTTPAHHCLVLGPGEGLSVWNKSS-K 242

Query: 249 TLRFILVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKS 300
            LRF+L+GG+P+NE VVQ GPFVMNT+ EI Q  +D++   NGFER+R W S
Sbjct: 243 PLRFVLIGGQPINEPVVQYGPFVMNTKSEIMQAYQDYQLGKNGFERSRQWYS 290

BLAST of CSPI03G04480 vs. ExPASy Swiss-Prot
Match: Q9ZW82 (Pirin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PRN2 PE=1 SV=3)

HSP 1 Score: 369.8 bits (948), Expect = 3.0e-101
Identity = 173/286 (60.49%), Postives = 218/286 (76.22%), Query Frame = 0

Query: 17  RFVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSAPGGFPDHPHRGFETV 76
           R V+KK  A+ Q EG GAVVRR I R E K  DPFL+LDEFSVS P GFPDHPHRGFETV
Sbjct: 37  RPVIKKVFAKLQKEGDGAVVRRGISRSEQKLLDPFLMLDEFSVSPPAGFPDHPHRGFETV 96

Query: 77  TYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIGTQRGLQLWINLSSKHK 136
           TY+L+G + H+DF+GHKG I  GD+QWMTAG+GI+HSE+P      +GLQLWINLSS  K
Sbjct: 97  TYVLEGGITHQDFKGHKGTIYAGDVQWMTAGRGIIHSEMPEE-EVNKGLQLWINLSSNEK 156

Query: 137 MIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPTMYLDFTLVPGSRIEQP 196
           MIEP YQE+ + +I +A + GV+V+VIAGE++G++SP+YT+TPTM+LDFTL PG++I Q 
Sbjct: 157 MIEPNYQELSHSDIPKAEQNGVEVKVIAGESMGIQSPVYTRTPTMFLDFTLQPGAQIHQN 216

Query: 197 IPTGWNAFVYVLEG--DGGVFGSMKLMPMVTPHHLLLLGNG-DGLEVWNKSSTKTLRFIL 256
           +P  WNAF Y+LE    GGVF S    P +  H +++ G G DG+ VWNKSS+K LRF+L
Sbjct: 217 VPESWNAFAYILESGEGGGVFSSSNSSP-IPAHSVVVFGPGNDGVSVWNKSSSKQLRFVL 276

Query: 257 VGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKS 300
           + GEP+ E VVQ GPFVMNTQ EID T+ED+    NGFE A++W+S
Sbjct: 277 IAGEPIGEPVVQYGPFVMNTQAEIDMTIEDYHYGKNGFEMAKYWRS 320

BLAST of CSPI03G04480 vs. ExPASy Swiss-Prot
Match: Q9LX49 (Pirin-1 OS=Arabidopsis thaliana OX=3702 GN=PRN1 PE=1 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 4.9e-96
Identity = 158/280 (56.43%), Postives = 210/280 (75.00%), Query Frame = 0

Query: 16  PRFVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSAPGGFPDHPHRGFET 75
           PR V+KK LA+ + EG GAVVR  I + + K  DPF++L EFS S   GFPDHPHRGFE+
Sbjct: 9   PRIVIKKVLAKLEKEGEGAVVRNGITKIDQKLLDPFVLLVEFSFSLSAGFPDHPHRGFES 68

Query: 76  VTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIGTQRGLQLWINLSSKH 135
           VTYMLQG +IH+D +GHKG I+ GD+QWMTAG+GI+HSE P       GLQLWINL S  
Sbjct: 69  VTYMLQGGIIHKDPKGHKGTIQAGDVQWMTAGRGIIHSEFPEE-EVNNGLQLWINLPSTE 128

Query: 136 KMIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPTMYLDFTLVPGSRIEQ 195
           KM EP+Y+E+ + +I  A   GV+V+VIAG+++G+KSP+YT+TPTM+LDFTL PGS+  Q
Sbjct: 129 KMTEPKYKELSSLDIPRAEENGVEVKVIAGDSMGIKSPVYTRTPTMFLDFTLKPGSQTHQ 188

Query: 196 PIPTGWNAFVYVLEGDGGVFGSMKLMPMVTPHHLLLLGNGDGLEVWNKSSTKTLRFILVG 255
            +P  W AF Y++EGD GVFGS+     ++ HH+++ G GD + VWNKS++++LRF+L+ 
Sbjct: 189 TVPESWTAFAYIIEGDEGVFGSLN-SSAISAHHVVVFGPGDLVSVWNKSTSRSLRFLLIA 248

Query: 256 GEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERAR 296
           GEP+ E VVQ GPFVMN+Q EID   +D++N  NGFE A+
Sbjct: 249 GEPIGEPVVQCGPFVMNSQAEIDMAFDDYQNAKNGFEMAK 286

BLAST of CSPI03G04480 vs. ExPASy Swiss-Prot
Match: O00625 (Pirin OS=Homo sapiens OX=9606 GN=PIR PE=1 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 3.0e-93
Identity = 161/282 (57.09%), Postives = 203/282 (71.99%), Query Frame = 0

Query: 19  VVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSAPGGFPDHPHRGFETVTY 78
           V    L+R Q EG+GA VRRSIGR ELK  DPFL+ DEF    PGGFPDHPHRGFETV+Y
Sbjct: 7   VTLSVLSREQSEGVGARVRRSIGRPELKNLDPFLLFDEFKGGRPGGFPDHPHRGFETVSY 66

Query: 79  MLQ-GAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIGTQRGLQLWINLSSKHKM 138
           +L+ G+M HEDF GH G++  GDLQWMTAG+GI+H+E+P S     GLQLW+NL S  KM
Sbjct: 67  LLEGGSMAHEDFCGHTGKMNPGDLQWMTAGRGILHAEMPCSEEPAHGLQLWVNLRSSEKM 126

Query: 139 IEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPTMYLDFTLVPGSRIEQPI 198
           +EP+YQE+ +E I + +++GV V VI+GEALG+KS +YT+TPT+YLDF L PG++  QPI
Sbjct: 127 VEPQYQELKSEEIPKPSKDGVTVAVISGEALGIKSKVYTRTPTLYLDFKLDPGAKHSQPI 186

Query: 199 PTGWNAFVYVLEGDGGVFGSMKLMPMVTPHHLLLLGNGDGLEVWNKSSTKTLRFILVGGE 258
           P GW +F+Y + GD  + G       + PHH  +LG GD ++V NK   K   F+L+ GE
Sbjct: 187 PKGWTSFIYTISGDVYI-GPDDAQQKIEPHHTAVLGEGDSVQVENK-DPKRSHFVLIAGE 246

Query: 259 PLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKS 300
           PL E V+Q GPFVMNT EEI Q + DF N  NGFERA+ WKS
Sbjct: 247 PLREPVIQHGPFVMNTNEEISQAILDFRNAKNGFERAKTWKS 286

BLAST of CSPI03G04480 vs. ExPASy TrEMBL
Match: A0A1S3BLA0 (pirin-like protein At1g50590 OS=Cucumis melo OX=3656 GN=LOC103491059 PE=3 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 4.8e-163
Identity = 282/299 (94.31%), Postives = 287/299 (95.99%), Query Frame = 0

Query: 2   MALHRNHLGSIIKEPRFVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSA 61
           MAL RN+LGS IKEPR VVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSA
Sbjct: 1   MALQRNNLGS-IKEPRSVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSA 60

Query: 62  PGGFPDHPHRGFETVTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIGT 121
           PGGFPDHPHRGFETVTYMLQGAMIHEDFEGHKGRIE GDLQWMTAGKGIVHSE+PSSIGT
Sbjct: 61  PGGFPDHPHRGFETVTYMLQGAMIHEDFEGHKGRIEGGDLQWMTAGKGIVHSEMPSSIGT 120

Query: 122 QRGLQLWINLSSKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPTM 181
           QRGLQLWINLSSKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALG KS I TKTPTM
Sbjct: 121 QRGLQLWINLSSKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGAKSSIDTKTPTM 180

Query: 182 YLDFTLVPGSRIEQPIPTGWNAFVYVLEGDGGVFGSMKLMPMVTPHHLLLLGNGDGLEVW 241
           YLDFTL PGSRIEQ IP GWNAFVYVLEGDGGVFGSMK MP VTPHHLLLLGNGDGLEVW
Sbjct: 181 YLDFTLAPGSRIEQSIPMGWNAFVYVLEGDGGVFGSMKSMPTVTPHHLLLLGNGDGLEVW 240

Query: 242 NKSSTKTLRFILVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKSS 301
           NKSS+KTLRFILVGG+PLNESVVQLGPFVMNTQEEIDQT+EDFENCTNGFERARHWKSS
Sbjct: 241 NKSSSKTLRFILVGGQPLNESVVQLGPFVMNTQEEIDQTIEDFENCTNGFERARHWKSS 298

BLAST of CSPI03G04480 vs. ExPASy TrEMBL
Match: A0A0A0L297 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G063110 PE=3 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 6.8e-141
Identity = 245/250 (98.00%), Postives = 247/250 (98.80%), Query Frame = 0

Query: 52  LVLDEFS-VSAPGGFPDHPHRGFETVTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGI 111
           L+L+E S VSAPGGFPDHPHRGFETVTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGI
Sbjct: 6   LLLEEASAVSAPGGFPDHPHRGFETVTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGI 65

Query: 112 VHSEIPSSIGTQRGLQLWINLSSKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGV 171
           VHSEIPSSIGTQRGLQLWINLSSKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGV
Sbjct: 66  VHSEIPSSIGTQRGLQLWINLSSKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGV 125

Query: 172 KSPIYTKTPTMYLDFTLVPGSRIEQPIPTGWNAFVYVLEGDGGVFGSMKLMPMVTPHHLL 231
           KSPIYTKTPTMYLDFTLVPGSRIEQPIPTGWNAFVYVLEGDGGVFGSMKLMP VTPHHLL
Sbjct: 126 KSPIYTKTPTMYLDFTLVPGSRIEQPIPTGWNAFVYVLEGDGGVFGSMKLMPTVTPHHLL 185

Query: 232 LLGNGDGLEVWNKSSTKTLRFILVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNG 291
           LLGNGDGLEVWNKSSTKTLRFILVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNG
Sbjct: 186 LLGNGDGLEVWNKSSTKTLRFILVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNG 245

Query: 292 FERARHWKSS 301
           FERARHWKSS
Sbjct: 246 FERARHWKSS 255

BLAST of CSPI03G04480 vs. ExPASy TrEMBL
Match: A0A6J1HKW0 (pirin-like protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465083 PE=3 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.3e-139
Identity = 234/287 (81.53%), Postives = 258/287 (89.90%), Query Frame = 0

Query: 13  IKEPRFVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSAPGGFPDHPHRG 72
           +K PR V + FLARPQHEG GAVVRRSIGR+ELKYFDPFLVLDEF V+AP GFPDHPHRG
Sbjct: 8   LKHPRLVDRTFLARPQHEGFGAVVRRSIGRYELKYFDPFLVLDEFFVTAPAGFPDHPHRG 67

Query: 73  FETVTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIGTQRGLQLWINLS 132
           FETVTYMLQGAM HEDF+GHKG I  GDLQWMTAGKGIVHSE+P+S GTQ GLQLWINLS
Sbjct: 68  FETVTYMLQGAMTHEDFQGHKGTIGAGDLQWMTAGKGIVHSEMPASHGTQHGLQLWINLS 127

Query: 133 SKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPTMYLDFTLVPGSR 192
           SKHKMIEPRYQE+HN ++VEA ++GVKVRVIAGEA+G +SPIYTKT TMYLDFTL PGS 
Sbjct: 128 SKHKMIEPRYQEVHNNDVVEAKKDGVKVRVIAGEAMGARSPIYTKTLTMYLDFTLDPGSH 187

Query: 193 IEQPIPTGWNAFVYVLEGDGGVFGSMKLMPMVTPHHLLLLGNGDGLEVWNKSSTKTLRFI 252
           IEQ IP GWNAFVYVLEG+ GVFGSMK MP +  HH++LLGNGDGLE WNKSS++ LRFI
Sbjct: 188 IEQSIPMGWNAFVYVLEGEDGVFGSMKSMP-IASHHIVLLGNGDGLEAWNKSSSEVLRFI 247

Query: 253 LVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKS 300
           LVGGEPLNESVVQ+GPFVMNT+EEIDQT+EDFENCTNGFERA+HW S
Sbjct: 248 LVGGEPLNESVVQMGPFVMNTEEEIDQTIEDFENCTNGFERAKHWSS 293

BLAST of CSPI03G04480 vs. ExPASy TrEMBL
Match: A0A6J1EVW0 (pirin-like protein isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111438618 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.7e-139
Identity = 233/287 (81.18%), Postives = 259/287 (90.24%), Query Frame = 0

Query: 13  IKEPRFVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSAPGGFPDHPHRG 72
           ++ PRFV +K LARPQHEG GAVVRRSIGR+ELKYFDPFLVLDEF V+ P GFPDHPHRG
Sbjct: 9   LERPRFVDRKLLARPQHEGFGAVVRRSIGRYELKYFDPFLVLDEFFVTPPAGFPDHPHRG 68

Query: 73  FETVTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIGTQRGLQLWINLS 132
           FETVTYMLQGAM HEDF+GHKG I  GDLQWMTAGKGIVHSE+P+S GTQ GLQLWINLS
Sbjct: 69  FETVTYMLQGAMTHEDFQGHKGTIGAGDLQWMTAGKGIVHSEMPASHGTQHGLQLWINLS 128

Query: 133 SKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPTMYLDFTLVPGSR 192
           SKHKMIEPRYQE+HN ++VEA ++GVKVRVIAGEA+G +SPIYTKTPTMYLDFTL PGS 
Sbjct: 129 SKHKMIEPRYQEVHNNDVVEAKKDGVKVRVIAGEAMGKRSPIYTKTPTMYLDFTLDPGSH 188

Query: 193 IEQPIPTGWNAFVYVLEGDGGVFGSMKLMPMVTPHHLLLLGNGDGLEVWNKSSTKTLRFI 252
           IEQPIP GWNAFVYVLEG+ GVFGS+K MP +  HH+LLLG+GDGLE WNKSS++ LRFI
Sbjct: 189 IEQPIPVGWNAFVYVLEGEDGVFGSVKSMP-IASHHILLLGSGDGLEAWNKSSSEALRFI 248

Query: 253 LVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKS 300
           LVGGEPLNESVVQ+GPFVMNT+EEIDQT+EDFEN TNGFERA+HW S
Sbjct: 249 LVGGEPLNESVVQMGPFVMNTEEEIDQTIEDFENGTNGFERAKHWSS 294

BLAST of CSPI03G04480 vs. ExPASy TrEMBL
Match: A0A5C7IEL3 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_008694 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 8.6e-136
Identity = 226/292 (77.40%), Postives = 259/292 (88.70%), Query Frame = 0

Query: 9   LGSIIKEPRFVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSAPGGFPDH 68
           L  ++KEPR VV+KFLARPQHEGLGA+VRRSIGRFEL+YFDPFLVLDEFSV+AP GFPDH
Sbjct: 7   LSCVVKEPRLVVRKFLARPQHEGLGAIVRRSIGRFELRYFDPFLVLDEFSVTAPAGFPDH 66

Query: 69  PHRGFETVTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIGTQRGLQLW 128
           PHRGFETVTYMLQGAM HEDFEGHKG I+ GDLQWMTAG+GIVHSE+P+  GTQ+GLQLW
Sbjct: 67  PHRGFETVTYMLQGAMTHEDFEGHKGTIKAGDLQWMTAGRGIVHSEMPAEQGTQKGLQLW 126

Query: 129 INLSSKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPTMYLDFTLV 188
           INLSSKHKMIEPRYQE+ +++I EA+++G+KVRVIAGEALG KSP+YT+TPTMYLDFTL 
Sbjct: 127 INLSSKHKMIEPRYQEVSSKDIAEASKDGIKVRVIAGEALGAKSPVYTRTPTMYLDFTLK 186

Query: 189 PGSRIEQPIPTGWNAFVYVLEGDGGVFGSMKLMPMVTPHHLLLLGN-GDGLEVWNKSSTK 248
           PG+ ++QPIP  WN+FVYVLEG+ G F   K  P V+ HHLLLLG+ GDGLE WNKS +K
Sbjct: 187 PGAHLQQPIPESWNSFVYVLEGE-GTFSGKKSSPPVSAHHLLLLGSGGDGLEAWNKSGSK 246

Query: 249 TLRFILVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKS 300
            LRFILVGGEPL E +VQ GPFVMNTQEEIDQTVEDFENC NGFE+A+HW+S
Sbjct: 247 PLRFILVGGEPLGEPLVQYGPFVMNTQEEIDQTVEDFENCVNGFEKAKHWRS 297

BLAST of CSPI03G04480 vs. NCBI nr
Match: XP_004149594.2 (pirin-like protein [Cucumis sativus] >KAE8650112.1 hypothetical protein Csa_010671 [Cucumis sativus])

HSP 1 Score: 621.7 bits (1602), Expect = 3.3e-174
Identity = 298/300 (99.33%), Postives = 298/300 (99.33%), Query Frame = 0

Query: 1   MMALHRNHLGSIIKEPRFVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVS 60
           MMALHRNHLGSIIKEPRFVVKKFLARPQHEGLGAVVRRSIGR ELKYFDPFLVLDEFSVS
Sbjct: 1   MMALHRNHLGSIIKEPRFVVKKFLARPQHEGLGAVVRRSIGRSELKYFDPFLVLDEFSVS 60

Query: 61  APGGFPDHPHRGFETVTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIG 120
           APGGFPDHPHRGFETVTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIG
Sbjct: 61  APGGFPDHPHRGFETVTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIG 120

Query: 121 TQRGLQLWINLSSKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPT 180
           TQRGLQLWINLSSKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPT
Sbjct: 121 TQRGLQLWINLSSKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPT 180

Query: 181 MYLDFTLVPGSRIEQPIPTGWNAFVYVLEGDGGVFGSMKLMPMVTPHHLLLLGNGDGLEV 240
           MYLDFTLVPGSRIEQPIPTGWNAFVYVLEGDGGVFGSMKLMP VTPHHLLLLGNGDGLEV
Sbjct: 181 MYLDFTLVPGSRIEQPIPTGWNAFVYVLEGDGGVFGSMKLMPTVTPHHLLLLGNGDGLEV 240

Query: 241 WNKSSTKTLRFILVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKSS 300
           WNKSSTKTLRFILVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKSS
Sbjct: 241 WNKSSTKTLRFILVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKSS 300

BLAST of CSPI03G04480 vs. NCBI nr
Match: XP_008449089.1 (PREDICTED: pirin-like protein At1g50590 [Cucumis melo])

HSP 1 Score: 583.6 bits (1503), Expect = 1.0e-162
Identity = 282/299 (94.31%), Postives = 287/299 (95.99%), Query Frame = 0

Query: 2   MALHRNHLGSIIKEPRFVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSA 61
           MAL RN+LGS IKEPR VVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSA
Sbjct: 1   MALQRNNLGS-IKEPRSVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSA 60

Query: 62  PGGFPDHPHRGFETVTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIGT 121
           PGGFPDHPHRGFETVTYMLQGAMIHEDFEGHKGRIE GDLQWMTAGKGIVHSE+PSSIGT
Sbjct: 61  PGGFPDHPHRGFETVTYMLQGAMIHEDFEGHKGRIEGGDLQWMTAGKGIVHSEMPSSIGT 120

Query: 122 QRGLQLWINLSSKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPTM 181
           QRGLQLWINLSSKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALG KS I TKTPTM
Sbjct: 121 QRGLQLWINLSSKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGAKSSIDTKTPTM 180

Query: 182 YLDFTLVPGSRIEQPIPTGWNAFVYVLEGDGGVFGSMKLMPMVTPHHLLLLGNGDGLEVW 241
           YLDFTL PGSRIEQ IP GWNAFVYVLEGDGGVFGSMK MP VTPHHLLLLGNGDGLEVW
Sbjct: 181 YLDFTLAPGSRIEQSIPMGWNAFVYVLEGDGGVFGSMKSMPTVTPHHLLLLGNGDGLEVW 240

Query: 242 NKSSTKTLRFILVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKSS 301
           NKSS+KTLRFILVGG+PLNESVVQLGPFVMNTQEEIDQT+EDFENCTNGFERARHWKSS
Sbjct: 241 NKSSSKTLRFILVGGQPLNESVVQLGPFVMNTQEEIDQTIEDFENCTNGFERARHWKSS 298

BLAST of CSPI03G04480 vs. NCBI nr
Match: XP_038905203.1 (pirin-like protein [Benincasa hispida])

HSP 1 Score: 540.0 bits (1390), Expect = 1.3e-149
Identity = 255/289 (88.24%), Postives = 269/289 (93.08%), Query Frame = 0

Query: 11  SIIKEPRFVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSAPGGFPDHPH 70
           S IK+PRF+VKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEF V+AP GFPDHPH
Sbjct: 7   SSIKDPRFLVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFFVTAPAGFPDHPH 66

Query: 71  RGFETVTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIGTQRGLQLWIN 130
           RGFETVTYMLQG M HEDF+GHKGRI  GDLQWMTAGKGIVHSE+P+S GTQ GLQLWIN
Sbjct: 67  RGFETVTYMLQGEMNHEDFKGHKGRIGAGDLQWMTAGKGIVHSEMPASHGTQHGLQLWIN 126

Query: 131 LSSKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPTMYLDFTLVPG 190
           LSSKHKMIEPRYQEIHNE++VEAT+EGVKVRVIAGEALG KS IYTKTPTMYLDFTL PG
Sbjct: 127 LSSKHKMIEPRYQEIHNEDVVEATKEGVKVRVIAGEALGAKSSIYTKTPTMYLDFTLSPG 186

Query: 191 SRIEQPIPTGWNAFVYVLEGDGGVFGSMKLMPMVTPHHLLLLGNGDGLEVWNKSSTKTLR 250
           SRIEQPIP GWNAFVYVLEG+GGVFGSMK MP + PHHLLLLGNGDGLEVWNK S+K LR
Sbjct: 187 SRIEQPIPLGWNAFVYVLEGEGGVFGSMKSMP-IMPHHLLLLGNGDGLEVWNKFSSKALR 246

Query: 251 FILVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKS 300
           FILVGG+PLNESVVQ GPFVMNTQEEIDQT+EDFENCTNGFERARHW S
Sbjct: 247 FILVGGQPLNESVVQFGPFVMNTQEEIDQTIEDFENCTNGFERARHWSS 294

BLAST of CSPI03G04480 vs. NCBI nr
Match: KAG6577495.1 (hypothetical protein SDJN03_25069, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015553.1 hypothetical protein SDJN02_23189 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 511.5 bits (1316), Expect = 4.8e-141
Identity = 235/287 (81.88%), Postives = 260/287 (90.59%), Query Frame = 0

Query: 13  IKEPRFVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSAPGGFPDHPHRG 72
           ++ PR V +K LARPQHEG GAVVRRSIGR+ELKYFDPFLVLDEF V+AP GFPDHPHRG
Sbjct: 9   LEHPRLVDRKLLARPQHEGFGAVVRRSIGRYELKYFDPFLVLDEFFVTAPAGFPDHPHRG 68

Query: 73  FETVTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIGTQRGLQLWINLS 132
           FETVTYMLQGAM HEDF+GHKG IE GDLQWMTAGKGIVHSE+P+S GTQ GLQLWINLS
Sbjct: 69  FETVTYMLQGAMTHEDFQGHKGTIEAGDLQWMTAGKGIVHSEMPASHGTQHGLQLWINLS 128

Query: 133 SKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPTMYLDFTLVPGSR 192
           SKHKMIEPRYQE+HN ++VEA ++GVKVRVIAGEA+G +SPIYTKTPTMYLDFTL PGS 
Sbjct: 129 SKHKMIEPRYQEVHNNDVVEAKKDGVKVRVIAGEAMGKRSPIYTKTPTMYLDFTLDPGSH 188

Query: 193 IEQPIPTGWNAFVYVLEGDGGVFGSMKLMPMVTPHHLLLLGNGDGLEVWNKSSTKTLRFI 252
           IEQPIP GWNAFVYVLEG+ GVFGS+K MP +  HH+LLLGNGDGLE WNKSS++ LRFI
Sbjct: 189 IEQPIPVGWNAFVYVLEGEDGVFGSVKSMP-IASHHILLLGNGDGLEAWNKSSSEALRFI 248

Query: 253 LVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKS 300
           LVGGEPLNESV Q+GPFVMNT+EEIDQT+EDFENCTNGFERA+HW S
Sbjct: 249 LVGGEPLNESVAQMGPFVMNTEEEIDQTIEDFENCTNGFERAKHWSS 294

BLAST of CSPI03G04480 vs. NCBI nr
Match: XP_023553374.1 (pirin-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 509.6 bits (1311), Expect = 1.8e-140
Identity = 234/287 (81.53%), Postives = 259/287 (90.24%), Query Frame = 0

Query: 13  IKEPRFVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSAPGGFPDHPHRG 72
           ++ PR V +K L RPQHEG GAVVRRSIGR+ELKYFDPFLVLDEF V+AP GFPDHPHRG
Sbjct: 9   LERPRLVDRKLLTRPQHEGFGAVVRRSIGRYELKYFDPFLVLDEFFVTAPAGFPDHPHRG 68

Query: 73  FETVTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIGTQRGLQLWINLS 132
           FETVTYMLQGAM HEDF+GHKG I  GDLQWMTAGKGIVHSE+P+S GTQ GLQLWINLS
Sbjct: 69  FETVTYMLQGAMTHEDFQGHKGTIGAGDLQWMTAGKGIVHSEMPASHGTQHGLQLWINLS 128

Query: 133 SKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPTMYLDFTLVPGSR 192
           SKHKMIEPRYQE+HN ++VEA ++GVKVRVIAGEA+G +SPIYTKTPTMYLDFTL PGS 
Sbjct: 129 SKHKMIEPRYQEVHNNDVVEAKKDGVKVRVIAGEAMGAQSPIYTKTPTMYLDFTLDPGSH 188

Query: 193 IEQPIPTGWNAFVYVLEGDGGVFGSMKLMPMVTPHHLLLLGNGDGLEVWNKSSTKTLRFI 252
           IEQPIP GWNAFVYVLEG+ GVFGSMK MP +  HH++LLGNGDGLE WNKSS++ LRFI
Sbjct: 189 IEQPIPVGWNAFVYVLEGEDGVFGSMKSMP-IASHHIVLLGNGDGLEAWNKSSSEALRFI 248

Query: 253 LVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKS 300
           LVGGEPLNESVVQ+GPFVMNT+EEIDQT+EDFENCTNGFERA+HW S
Sbjct: 249 LVGGEPLNESVVQMGPFVMNTEEEIDQTIEDFENCTNGFERAKHWSS 294

BLAST of CSPI03G04480 vs. TAIR 10
Match: AT1G50590.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 407.9 bits (1047), Expect = 7.0e-114
Identity = 204/290 (70.34%), Postives = 228/290 (78.62%), Query Frame = 0

Query: 17  RFVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSAPGGFPDHPHRGFETV 76
           R VVKK  AR  HEG GAVVRRSIGRFE +YFDPFLVLDEFSVSAP GFPDHPHRGFETV
Sbjct: 13  RLVVKKLFARQLHEGFGAVVRRSIGRFEFRYFDPFLVLDEFSVSAPAGFPDHPHRGFETV 72

Query: 77  TYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSS----IGTQRGLQLWINLS 136
           TYML+G ++HED EGHKG I  G LQWMTAGKGIVHSE+PSS    I   +GLQLWINLS
Sbjct: 73  TYMLEGEILHEDCEGHKGVIREGGLQWMTAGKGIVHSEMPSSNSNGITHNKGLQLWINLS 132

Query: 137 SKHKMIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPTMYLDFTLVPGSR 196
           S+ K++EP YQEI +++I E  ++GV+VRVIAGE  GVKS I T+TPTMYLDFTL PGSR
Sbjct: 133 SRQKLVEPSYQEIESKDIAETEKDGVRVRVIAGEWNGVKSKICTRTPTMYLDFTLSPGSR 192

Query: 197 IEQPIPTGWNAFVYVLEGDGGVFGSMKLM-PMVTPHHLLLLG-NGDGLEVWNKS-STKTL 256
           I QPIP  WNAFVYVL+G G  FG  KL       HHLL+LG  GD LE WN S S   L
Sbjct: 193 ISQPIPLHWNAFVYVLQGHGH-FGDSKLQHSAAAAHHLLVLGLGGDMLEAWNGSDSGLPL 252

Query: 257 RFILVGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKS 300
           RFILV GEP+ E +VQ GPFVMNTQEEID+T++DFEN  NGFE+ARHWKS
Sbjct: 253 RFILVAGEPIGEPMVQFGPFVMNTQEEIDETIDDFENFRNGFEKARHWKS 301

BLAST of CSPI03G04480 vs. TAIR 10
Match: AT2G43120.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 369.8 bits (948), Expect = 2.1e-102
Identity = 173/286 (60.49%), Postives = 218/286 (76.22%), Query Frame = 0

Query: 17  RFVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSAPGGFPDHPHRGFETV 76
           R V+KK  A+ Q EG GAVVRR I R E K  DPFL+LDEFSVS P GFPDHPHRGFETV
Sbjct: 37  RPVIKKVFAKLQKEGDGAVVRRGISRSEQKLLDPFLMLDEFSVSPPAGFPDHPHRGFETV 96

Query: 77  TYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIGTQRGLQLWINLSSKHK 136
           TY+L+G + H+DF+GHKG I  GD+QWMTAG+GI+HSE+P      +GLQLWINLSS  K
Sbjct: 97  TYVLEGGITHQDFKGHKGTIYAGDVQWMTAGRGIIHSEMPEE-EVNKGLQLWINLSSNEK 156

Query: 137 MIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPTMYLDFTLVPGSRIEQP 196
           MIEP YQE+ + +I +A + GV+V+VIAGE++G++SP+YT+TPTM+LDFTL PG++I Q 
Sbjct: 157 MIEPNYQELSHSDIPKAEQNGVEVKVIAGESMGIQSPVYTRTPTMFLDFTLQPGAQIHQN 216

Query: 197 IPTGWNAFVYVLEG--DGGVFGSMKLMPMVTPHHLLLLGNG-DGLEVWNKSSTKTLRFIL 256
           +P  WNAF Y+LE    GGVF S    P +  H +++ G G DG+ VWNKSS+K LRF+L
Sbjct: 217 VPESWNAFAYILESGEGGGVFSSSNSSP-IPAHSVVVFGPGNDGVSVWNKSSSKQLRFVL 276

Query: 257 VGGEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERARHWKS 300
           + GEP+ E VVQ GPFVMNTQ EID T+ED+    NGFE A++W+S
Sbjct: 277 IAGEPIGEPVVQYGPFVMNTQAEIDMTIEDYHYGKNGFEMAKYWRS 320

BLAST of CSPI03G04480 vs. TAIR 10
Match: AT3G59220.1 (pirin )

HSP 1 Score: 352.4 bits (903), Expect = 3.5e-97
Identity = 158/280 (56.43%), Postives = 210/280 (75.00%), Query Frame = 0

Query: 16  PRFVVKKFLARPQHEGLGAVVRRSIGRFELKYFDPFLVLDEFSVSAPGGFPDHPHRGFET 75
           PR V+KK LA+ + EG GAVVR  I + + K  DPF++L EFS S   GFPDHPHRGFE+
Sbjct: 9   PRIVIKKVLAKLEKEGEGAVVRNGITKIDQKLLDPFVLLVEFSFSLSAGFPDHPHRGFES 68

Query: 76  VTYMLQGAMIHEDFEGHKGRIEVGDLQWMTAGKGIVHSEIPSSIGTQRGLQLWINLSSKH 135
           VTYMLQG +IH+D +GHKG I+ GD+QWMTAG+GI+HSE P       GLQLWINL S  
Sbjct: 69  VTYMLQGGIIHKDPKGHKGTIQAGDVQWMTAGRGIIHSEFPEE-EVNNGLQLWINLPSTE 128

Query: 136 KMIEPRYQEIHNENIVEATREGVKVRVIAGEALGVKSPIYTKTPTMYLDFTLVPGSRIEQ 195
           KM EP+Y+E+ + +I  A   GV+V+VIAG+++G+KSP+YT+TPTM+LDFTL PGS+  Q
Sbjct: 129 KMTEPKYKELSSLDIPRAEENGVEVKVIAGDSMGIKSPVYTRTPTMFLDFTLKPGSQTHQ 188

Query: 196 PIPTGWNAFVYVLEGDGGVFGSMKLMPMVTPHHLLLLGNGDGLEVWNKSSTKTLRFILVG 255
            +P  W AF Y++EGD GVFGS+     ++ HH+++ G GD + VWNKS++++LRF+L+ 
Sbjct: 189 TVPESWTAFAYIIEGDEGVFGSLN-SSAISAHHVVVFGPGDLVSVWNKSTSRSLRFLLIA 248

Query: 256 GEPLNESVVQLGPFVMNTQEEIDQTVEDFENCTNGFERAR 296
           GEP+ E VVQ GPFVMN+Q EID   +D++N  NGFE A+
Sbjct: 249 GEPIGEPVVQCGPFVMNSQAEIDMAFDDYQNAKNGFEMAK 286

BLAST of CSPI03G04480 vs. TAIR 10
Match: AT3G59260.1 (pirin, putative )

HSP 1 Score: 312.8 bits (800), Expect = 3.1e-85
Identity = 145/263 (55.13%), Postives = 189/263 (71.86%), Query Frame = 0

Query: 37  RRSIGRFELKYFDPFLVLDEFSVSAPGGFPDHPHRGFETVTYMLQGAMIHEDFEGHKGRI 96
           + S  + + +  DPF+ L EFSVS PGGF DHPHRGFE+VTYM QG +IH+D  G+KG I
Sbjct: 10  KASPSKSDHELLDPFVSLVEFSVSPPGGFKDHPHRGFESVTYMFQGGIIHQDCNGNKGTI 69

Query: 97  EVGDLQWMTAGKGIVHSEIPSSIGTQRGLQLWINLSSKHKMIEPRYQEIHNENIVEATRE 156
             GD+QWMTAG+GI+HSE+P      +GLQLWINL S  KMIEP+  EI +  I  A   
Sbjct: 70  HEGDVQWMTAGRGIIHSEMPEE-QVNKGLQLWINLPSSAKMIEPKNIEISSSEIPSADDY 129

Query: 157 GVKVRVIAGEALGVKSPIYTKTPTMYLDFTLVPGSRIEQPIPTGWNAFVYVLEGDGGVFG 216
           GV+V+VIAGE++GVKSP YTKTP M+LDFTL P ++  Q +P  W AF Y++EGD GVF 
Sbjct: 130 GVEVKVIAGESMGVKSPFYTKTPIMFLDFTLDPKAQTHQAVPESWTAFAYIVEGDEGVFS 189

Query: 217 SMKLMPMVTPHHLLLLGNGDGLEVWNKSSTKTLRFILVGGEPLNESVVQLGPFVMNTQEE 276
           S      V  H++++ G GD + VWN S+++ LRF+L+ GEP+ E VVQ GPFVMN+Q+E
Sbjct: 190 SSD-SSTVQAHNVVVFGTGDEVSVWNTSNSRPLRFLLIAGEPIGEPVVQHGPFVMNSQDE 249

Query: 277 IDQTVEDFENCTNGFERARHWKS 300
           I+ T+ D+ N  NGFE A+HW+S
Sbjct: 250 IEMTIGDYRNGMNGFEMAKHWRS 270

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LPS99.9e-11370.34Pirin-like protein At1g50590 OS=Arabidopsis thaliana OX=3702 GN=At1g50590 PE=2 S... [more]
Q9SEE42.7e-11065.41Pirin-like protein OS=Solanum lycopersicum OX=4081 PE=2 SV=1[more]
Q9ZW823.0e-10160.49Pirin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PRN2 PE=1 SV=3[more]
Q9LX494.9e-9656.43Pirin-1 OS=Arabidopsis thaliana OX=3702 GN=PRN1 PE=1 SV=1[more]
O006253.0e-9357.09Pirin OS=Homo sapiens OX=9606 GN=PIR PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BLA04.8e-16394.31pirin-like protein At1g50590 OS=Cucumis melo OX=3656 GN=LOC103491059 PE=3 SV=1[more]
A0A0A0L2976.8e-14198.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G063110 PE=3 SV=1[more]
A0A6J1HKW01.3e-13981.53pirin-like protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465083 PE=3 S... [more]
A0A6J1EVW03.7e-13981.18pirin-like protein isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111438618 PE=3... [more]
A0A5C7IEL38.6e-13677.40Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_008694 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004149594.23.3e-17499.33pirin-like protein [Cucumis sativus] >KAE8650112.1 hypothetical protein Csa_0106... [more]
XP_008449089.11.0e-16294.31PREDICTED: pirin-like protein At1g50590 [Cucumis melo][more]
XP_038905203.11.3e-14988.24pirin-like protein [Benincasa hispida][more]
KAG6577495.14.8e-14181.88hypothetical protein SDJN03_25069, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023553374.11.8e-14081.53pirin-like protein [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G50590.17.0e-11470.34RmlC-like cupins superfamily protein [more]
AT2G43120.12.1e-10260.49RmlC-like cupins superfamily protein [more]
AT3G59220.13.5e-9756.43pirin [more]
AT3G59260.13.1e-8555.13pirin, putative [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003829Pirin, N-terminal domainPFAMPF02678Pirincoord: 36..129
e-value: 3.9E-29
score: 100.8
IPR008778Pirin, C-terminal domainPFAMPF05726Pirin_Ccoord: 182..290
e-value: 3.7E-33
score: 114.1
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 151..259
e-value: 2.9E-103
score: 347.0
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 30..285
e-value: 2.9E-103
score: 347.0
IPR012093PirinPIRSFPIRSF006232Pirincoord: 15..298
e-value: 7.1E-97
score: 322.0
IPR012093PirinPANTHERPTHR13903PIRIN-RELATEDcoord: 11..300
NoneNo IPR availablePANTHERPTHR13903:SF26SUBFAMILY NOT NAMEDcoord: 11..300
NoneNo IPR availableCDDcd02247cupin_pirin_Ccoord: 179..258
e-value: 3.63183E-16
score: 69.9526
NoneNo IPR availableCDDcd02909cupin_pirin_Ncoord: 34..131
e-value: 3.16745E-54
score: 170.024
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 17..298

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G04480.1CSPI03G04480.1mRNA