CSPI03G04350 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G04350
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Calcium-binding protein
Location	Chr3: 3552484 .. 3554813 (+)
RNA-Seq Expression	CSPI03G04350
Synteny	CSPI03G04350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTTTGGACTGTTTTATGTATAATTAATCTTTCCTATCTTCCAGGGAGATTGTCGAACTTAGAGCCGATACACAAACGTGGTGGGTTGTCCACAAAGATCGGTTCAAGAATCCAATGAAGCATCAGCAAAGAATCACCATTATTCTCCATTTTTATCTTCATCTCATCAACAACTAATCTCTACCAAACTTTTCCCTTTTCTCTTCTTTCATCTACGACAAAACCCCACCAAAAGAATCTCCCAAAACACCCTCTAATTTCTCCTCTTTTTTTCTTTTTTTTTTTTCTTTTGTTGACCTTCTCTGATTCCTGAAGAAGAACCCATTTTGGAATCAAGCCTTGCCTTAATCTCTAACAGGTTAAACCTTCTTCCTTCCTTCCTTCCTTTTAATTTTTTTTTTTTTTTGTTTCATTGATTATGGTTTCAGTTATTGGTTCTCTTAGGATTTTGATTTCTGGTTCTGTTCTTCAAGTGTTGAAATGAATATGGGCATGTTTGGTGGTTTTGTTATCTTCCTTTTGTTTGTTGGTTCATGTGTTTATAGACCCTTTTCTTGGATTTTATCTTAATATTGAACCCTTTTGTGTTTTCTCAAGATTTTGAACCAGTTTACATCTTTTTGTGTTAACCCTTTTCTTCTTTTTAATTGATAACTTGCAAGCATTCATCCATGATAAATATGAAGCTAAGATGTAATGTTTCAAAATTTTAAAAGCTGAGTACTAAAGTATAATATAATAGACCTTTTTCTTTATTGAATGATTCTTTTAACACTTGGTTTTTTATGGTGTTGAGTAGACTGTTTGATTGTTTGTTTTATGTCTATGAACAGAGACATTGTTGAATTAGTTGGCAATGGGGAGTGTGGTGGGGAAGTTGGAATCTCCTAGGGAATGTGTACCAGAAACAAAACTTGAAGCCAAAATGGTTGAGACAATGAAACAAAGAGCAACTAAAGGAAGCATCATTAGGTCATTTGATTGCATAATCTTGAAATTCCCCAAAATCGATGATAGCCTTAGAAACTGCAAAACTATTTTCCAACAGTTTGGTGAGTAACTTTGTTTGGTTCTGTTCTTTAGAATGCCTATTTAACTATATGGTTACTTAATGCCACAGTTTAAAACTTCAAGTTTCCAAGTCTTGAATTTACAATTACTCTACTAGTACCCCACAATATAGTGATGATCTTGATGGTGATACTTAGAAGTTATGGCTTCTTTGTAGATGAGGATTTGAATGGGATAATTGATCGTCGGGAGCTTAAAAAATGCTTTGACGGGCTGGAGATTTTGCTTACAGAGGAGGAAATCGATGATCTCTTTGATGCTTGTGATATTAGTACAGCTATGGGAATGAAGTTCAATGAATTCATTGTACTTCTCTGCCTTGTCTATCTTCTCAAGGATGATCCCAATGCTGTATTTTCTGTATCCGAATGTTTTGTTTTCAAGCTGCTTGTTTCTTCATTCTTTTTCTCTATGCTTTTCCTTTTGTATTCCCTGACTTCAACAGAAATCCCAATTCGGAATGCCAAAATTGGAGCAGACATTTGAATCATTGGTTGATGCATTCGTGTTCTTGGACAAGAATAAGGATGGATACGTAAGCAAGAGCGAGATGGTATCTGCAATAAATGAAACCACGTCGGGAGAACGTTCTTCAGGGCGGATAGCAATGAGGAGATTCGGTAATACTCATTGACCCTTTGCTCTTTCCATTTGAATCTGATTGCTGCACCAATGAATTAATGGTTGGACTTGTTTCACTCATTTGATCTTGGCAGAGGAGATGGACTGGGACAAAAATGGAATGGTAAACTTCAAAGAATTTCTCTTCGCATTCACCCGTTGGGTTGGAATCGACGAGAATGAGGACGGAGAGGAGGAAGAGGAAGAATGAAAGATGAAAATCATTTCATCTTGCATACGAAACTTCTTTTCCCAATTCCTCGTTTGATGATGGAGAAACTGTCGTGCACAGTAGTGAAAGAAAGGATGGTGTTTCATCTGCACTCTGTTGGCCTTTCTCACTCAAAAAGTGAACTAGAAGATAACAATGGTTTCAAGGCATTAGTAGTAAATAATTGTGTGAACTTGTGAAGAGTAGAAGTCATATAATTTGAAATGCTATTGTTGAAGCCAAGCCTGAGCCTTTTTTTTCAAGTGGGGATTGTTTTTGTTTATTATTATTCTCCATGGCAGCTTCACTGATTTTCACTGGATGGGTTGGAAAGTAGTTGTATTTTCTTTGGACCTCTCTGACTTGCTCGCAATATTATGGACTCCTTTTCTTCTTATTCAAAGGGAAACTTCACTTCATCGCCTG

mRNA sequence

CTTTTTGGACTGTTTTATGTATAATTAATCTTTCCTATCTTCCAGGGAGATTGTCGAACTTAGAGCCGATACACAAACGTGGTGGGTTGTCCACAAAGATCGGTTCAAGAATCCAATGAAGCATCAGCAAAGAATCACCATTATTCTCCATTTTTATCTTCATCTCATCAACAACTAATCTCTACCAAACTTTTCCCTTTTCTCTTCTTTCATCTACGACAAAACCCCACCAAAAGAATCTCCCAAAACACCCTCTAATTTCTCCTCTTTTTTTCTTTTTTTTTTTTCTTTTGTTGACCTTCTCTGATTCCTGAAGAAGAACCCATTTTGGAATCAAGCCTTGCCTTAATCTCTAACAGAGACATTGTTGAATTAGTTGGCAATGGGGAGTGTGGTGGGGAAGTTGGAATCTCCTAGGGAATGTGTACCAGAAACAAAACTTGAAGCCAAAATGGTTGAGACAATGAAACAAAGAGCAACTAAAGGAAGCATCATTAGGTCATTTGATTGCATAATCTTGAAATTCCCCAAAATCGATGATAGCCTTAGAAACTGCAAAACTATTTTCCAACAGTTTGATGAGGATTTGAATGGGATAATTGATCGTCGGGAGCTTAAAAAATGCTTTGACGGGCTGGAGATTTTGCTTACAGAGGAGGAAATCGATGATCTCTTTGATGCTTGTGATATTAGTACAGCTATGGGAATGAAGTTCAATGAATTCATTGTACTTCTCTGCCTTGTCTATCTTCTCAAGGATGATCCCAATGCTGTATTTTCTAAATCCCAATTCGGAATGCCAAAATTGGAGCAGACATTTGAATCATTGGTTGATGCATTCGTGTTCTTGGACAAGAATAAGGATGGATACGTAAGCAAGAGCGAGATGGTATCTGCAATAAATGAAACCACGTCGGGAGAACGTTCTTCAGGGCGGATAGCAATGAGGAGATTCGAGGAGATGGACTGGGACAAAAATGGAATGGTAAACTTCAAAGAATTTCTCTTCGCATTCACCCGTTGGGTTGGAATCGACGAGAATGAGGACGGAGAGGAGGAAGAGGAAGAATGAAAGATGAAAATCATTTCATCTTGCATACGAAACTTCTTTTCCCAATTCCTCGTTTGATGATGGAGAAACTGTCGTGCACAGTAGTGAAAGAAAGGATGGTGTTTCATCTGCACTCTGTTGGCCTTTCTCACTCAAAAAGTGAACTAGAAGATAACAATGGTTTCAAGGCATTAGTAGTAAATAATTGTGTGAACTTGTGAAGAGTAGAAGTCATATAATTTGAAATGCTATTGTTGAAGCCAAGCCTGAGCCTTTTTTTTCAAGTGGGGATTGTTTTTGTTTATTATTATTCTCCATGGCAGCTTCACTGATTTTCACTGGATGGGTTGGAAAGTAGTTGTATTTTCTTTGGACCTCTCTGACTTGCTCGCAATATTATGGACTCCTTTTCTTCTTATTCAAAGGGAAACTTCACTTCATCGCCTG

Coding sequence (CDS)

ATGGGGAGTGTGGTGGGGAAGTTGGAATCTCCTAGGGAATGTGTACCAGAAACAAAACTTGAAGCCAAAATGGTTGAGACAATGAAACAAAGAGCAACTAAAGGAAGCATCATTAGGTCATTTGATTGCATAATCTTGAAATTCCCCAAAATCGATGATAGCCTTAGAAACTGCAAAACTATTTTCCAACAGTTTGATGAGGATTTGAATGGGATAATTGATCGTCGGGAGCTTAAAAAATGCTTTGACGGGCTGGAGATTTTGCTTACAGAGGAGGAAATCGATGATCTCTTTGATGCTTGTGATATTAGTACAGCTATGGGAATGAAGTTCAATGAATTCATTGTACTTCTCTGCCTTGTCTATCTTCTCAAGGATGATCCCAATGCTGTATTTTCTAAATCCCAATTCGGAATGCCAAAATTGGAGCAGACATTTGAATCATTGGTTGATGCATTCGTGTTCTTGGACAAGAATAAGGATGGATACGTAAGCAAGAGCGAGATGGTATCTGCAATAAATGAAACCACGTCGGGAGAACGTTCTTCAGGGCGGATAGCAATGAGGAGATTCGAGGAGATGGACTGGGACAAAAATGGAATGGTAAACTTCAAAGAATTTCTCTTCGCATTCACCCGTTGGGTTGGAATCGACGAGAATGAGGACGGAGAGGAGGAAGAGGAAGAATGA

Protein sequence

MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKTIFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCLVYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGERSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEEEE*

Homology

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BLAST of CSPI03G04350 vs. ExPASy Swiss-Prot
Match: Q52K82 (Probable calcium-binding protein CML21 OS=Arabidopsis thaliana OX=3702 GN=CML21 PE=2 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 7.4e-84
Identity = 158/228 (69.30%), Postives = 186/228 (81.58%), Query Frame = 0


Query: 1   MGSVVGKLES-PRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCK 60
           MG  V K E+  +E VPETKLEAK++E +++RA++G+ ++SF+ I+LKFPKIDD LRNCK
Sbjct: 1   MGGAVTKSETLQKEWVPETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCK 60

Query: 61  TIFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLC 120
            IFQ+FDED NG ID  ELK C   LEI   EEEI+DLF ACDI+  MG+ F EFIVLLC
Sbjct: 61  AIFQEFDEDSNGSIDHTELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLC 120

Query: 121 LVYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG 180
           LVYLLKDD + +  K   GMPKLE TFE+LVD FVFLD+NKDGYVS+ EMV AI+E  SG
Sbjct: 121 LVYLLKDDSSTLQKKWTMGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDE--SG 180

Query: 181 ERSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEE 228
           ERSSGRIAM+RFEEMDWDKNGMVNFKEFLFAFT+WVGIDENE+ EEE+
Sbjct: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTQWVGIDENEEEEEED 226

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BLAST of CSPI03G04350 vs. ExPASy Swiss-Prot
Match: Q9LRN6 (Probable calcium-binding protein CML22 OS=Arabidopsis thaliana OX=3702 GN=CML22 PE=3 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 4.4e-44
Identity = 91/208 (43.75%), Postives = 137/208 (65.87%), Query Frame = 0


Query: 17  ETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKTIFQQFDEDLNGIIDRR 76
           + KL  KMVE+        S ++S D II+KFPK+ + LRN +++F+ +D D NG ID  
Sbjct: 18  DAKLARKMVESRSIYPGHRS-LKSMDSIIMKFPKLREGLRNIRSVFESYDNDTNGTIDIE 77

Query: 77  ELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCLVYLLKDDPNAVFSKSQ 136
           ELKKC + L++ L++EE+  L+  CD+  + G++FNEFIVLLCL+YLL    +   ++S+
Sbjct: 78  ELKKCLEELKLSLSDEEVKGLYSWCDVDGSKGIQFNEFIVLLCLIYLLAKPSSESSTESR 137

Query: 137 FGMPKL-EQTFESLVDAFVFLDKNKDGYVSKSEMVSAI-NETTSGERSSGRIAMRRFEEM 196
              PKL E  F+ +V+ F+FLDK+  G ++K++++  + NE    ERS   +   RFEEM
Sbjct: 138 EMGPKLVESIFDPIVEVFLFLDKDGKGKLNKADVIKTLNNEDYPLERSPSHVTNMRFEEM 197

Query: 197 DWDKNGMVNFKEFLFAFTRWVGIDENED 223
           DW + G V F+EFLFAF  WVG+D+ +D
Sbjct: 198 DWGRKGKVGFREFLFAFMSWVGLDDADD 224

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BLAST of CSPI03G04350 vs. ExPASy Swiss-Prot
Match: Q9M101 (Calcium-dependent protein kinase 23 OS=Arabidopsis thaliana OX=3702 GN=CPK23 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.6e-09
Identity = 49/195 (25.13%), Postives = 90/195 (46.15%), Query Frame = 0


Query: 16  PETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKI---DDSLRNCKTIFQQFDEDLNGI 75
           PE  +++ ++  MKQ      + +    + LK   +   ++ ++  KT+F   D + +G 
Sbjct: 333 PEKPIDSTVLSRMKQFRAMNKLKK----LALKVSAVSLSEEEIKGLKTLFANMDTNRSGT 392

Query: 76  IDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCLVYLLKDDPNAVF 135
           I   +L+     L   L+E E+  L +A D+     + + EFI      Y L  D     
Sbjct: 393 ITYEQLQTGLSRLRSRLSETEVQQLVEASDVDGNGTIDYYEFISATMHRYKLHHD----- 452

Query: 136 SKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGERSSGRIAMRRFE 195
                         E +  AF  LDK+K+G++++ E+ SA+ E   G+ +S +  +    
Sbjct: 453 --------------EHVHKAFQHLDKDKNGHITRDELESAMKEYGMGDEASIKEVI---S 501

Query: 196 EMDWDKNGMVNFKEF 208
           E+D D +G +NF+EF
Sbjct: 513 EVDTDNDGKINFEEF 501

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BLAST of CSPI03G04350 vs. ExPASy Swiss-Prot
Match: O49717 (Calcium-dependent protein kinase 15 OS=Arabidopsis thaliana OX=3702 GN=CPK15 PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 5.9e-09
Identity = 46/192 (23.96%), Postives = 87/192 (45.31%), Query Frame = 0


Query: 16  PETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKTIFQQFDEDLNGIIDR 75
           P+  +++ ++  MKQ       ++     ++     ++ ++  KT+F   D D +G I  
Sbjct: 366 PDKPIDSAVLSRMKQFRAMNK-LKKLALKVIAESLSEEEIKGLKTMFANMDTDKSGTITY 425

Query: 76  RELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCLVYLLKDDPNAVFSKS 135
            ELK     L   LTE E+  L +A D+     + + EFI             +A   + 
Sbjct: 426 EELKNGLAKLGSKLTEAEVKQLMEAADVDGNGTIDYIEFI-------------SATMHRY 485

Query: 136 QFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGERSSGRIAMRRFEEMD 195
           +F         E +  AF + DK+  G+++  E+ SA+ E   G+ +S +  +    E+D
Sbjct: 486 RFDRD------EHVFKAFQYFDKDNSGFITMDELESAMKEYGMGDEASIKEVI---AEVD 534

Query: 196 WDKNGMVNFKEF 208
            D +G +N++EF
Sbjct: 546 TDNDGRINYEEF 534

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BLAST of CSPI03G04350 vs. ExPASy Swiss-Prot
Match: Q9ZSA2 (Calcium-dependent protein kinase 21 OS=Arabidopsis thaliana OX=3702 GN=CPK21 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.0e-08
Identity = 47/192 (24.48%), Postives = 85/192 (44.27%), Query Frame = 0


Query: 16  PETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKTIFQQFDEDLNGIIDR 75
           P+  +++ ++  MKQ       ++     ++     ++ ++  KT+F   D D +G I  
Sbjct: 344 PDKPIDSAVLSRMKQFRAMNK-LKKLALKVIAESLSEEEIKGLKTMFANIDTDKSGTITY 403

Query: 76  RELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCLVYLLKDDPNAVFSKS 135
            ELK     L   L+E E+  L +A D+     + + EFI      Y L  D        
Sbjct: 404 EELKTGLTRLGSRLSETEVKQLMEAADVDGNGTIDYYEFISATMHRYKLDRD-------- 463

Query: 136 QFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGERSSGRIAMRRFEEMD 195
                      E +  AF   DK+  G++++ E+ SA+ E   G+ +S +  +    E+D
Sbjct: 464 -----------EHVYKAFQHFDKDNSGHITRDELESAMKEYGMGDEASIKEVI---SEVD 512

Query: 196 WDKNGMVNFKEF 208
            D +G +NF+EF
Sbjct: 524 TDNDGRINFEEF 512

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BLAST of CSPI03G04350 vs. ExPASy TrEMBL
Match: A0A0A0L7G7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G061000 PE=4 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 4.0e-125
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 0


Query: 1   MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT 60
           MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT
Sbjct: 1   MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT 60

Query: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
           IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL
Sbjct: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120

Query: 121 VYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE 180
           VYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE
Sbjct: 121 VYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE 180

Query: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEEEE 230
           RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEEEE
Sbjct: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEEEE 229

[Close]

BLAST of CSPI03G04350 vs. ExPASy TrEMBL
Match: A0A1S3BLU5 (probable calcium-binding protein CML21 OS=Cucumis melo OX=3656 GN=LOC103491047 PE=4 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 6.2e-118
Identity = 213/224 (95.09%), Postives = 222/224 (99.11%), Query Frame = 0


Query: 1   MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT 60
           MGSVVGKLESPRECVPETKLEAKMVETM++RATKGSIIRSFDCI+LKFPKIDDSLRNCKT
Sbjct: 1   MGSVVGKLESPRECVPETKLEAKMVETMRRRATKGSIIRSFDCILLKFPKIDDSLRNCKT 60

Query: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
           IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLF+ACDIS AMG+KFNEFIVLLCL
Sbjct: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISAAMGIKFNEFIVLLCL 120

Query: 121 VYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE 180
           VYLLKDDP+AVFSKSQFGMPKLE+TFESLVDAFVFLDKNKDGYVSKSEM+SAINETTSGE
Sbjct: 121 VYLLKDDPDAVFSKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMISAINETTSGE 180

Query: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGE 225
           RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENE+ E
Sbjct: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEEEE 224

[Close]

BLAST of CSPI03G04350 vs. ExPASy TrEMBL
Match: A0A6J1KV85 (probable calcium-binding protein CML21 OS=Cucurbita maxima OX=3661 GN=LOC111498495 PE=4 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 6.2e-110
Identity = 204/227 (89.87%), Postives = 214/227 (94.27%), Query Frame = 0


Query: 1   MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT 60
           MGSVVGKLES  ECVPETKLEAKMVE ++QRA+KGSIIRSFDCIILKFPKID SLR CKT
Sbjct: 1   MGSVVGKLESHSECVPETKLEAKMVEAIRQRASKGSIIRSFDCIILKFPKIDGSLRKCKT 60

Query: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
           IF++FDED NGIIDRRELK+CFDGLEI LT+EEIDDLFDACD+STAMG+KFNEFIVLLCL
Sbjct: 61  IFEKFDEDSNGIIDRRELKECFDGLEISLTQEEIDDLFDACDMSTAMGIKFNEFIVLLCL 120

Query: 121 VYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE 180
           VYLLKDDPN    KSQFGMPKLE+TFESLVDAFVFLD NKDGYVSKSEMVSAINETTSGE
Sbjct: 121 VYLLKDDPN---HKSQFGMPKLEETFESLVDAFVFLDTNKDGYVSKSEMVSAINETTSGE 180

Query: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEE 228
           RSSGRI MRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEE
Sbjct: 181 RSSGRIGMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEE 224

[Close]

BLAST of CSPI03G04350 vs. ExPASy TrEMBL
Match: A0A6J1G727 (probable calcium-binding protein CML21 OS=Cucurbita moschata OX=3662 GN=LOC111451446 PE=4 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 8.1e-110
Identity = 203/227 (89.43%), Postives = 214/227 (94.27%), Query Frame = 0


Query: 1   MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT 60
           MGSVVGKLES RECVPETKLEAKMVE ++QRA+KGSIIRSFDCIILKFPKID SLR CKT
Sbjct: 1   MGSVVGKLESQRECVPETKLEAKMVEAIRQRASKGSIIRSFDCIILKFPKIDASLRKCKT 60

Query: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
           IF++FDED NGIIDRRELK+CFDGLEI LT+EEIDDLFDACD+STAMG+KFNEFIVLLCL
Sbjct: 61  IFEKFDEDSNGIIDRRELKECFDGLEISLTQEEIDDLFDACDMSTAMGIKFNEFIVLLCL 120

Query: 121 VYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE 180
           VYLLKDDPN    KSQFGMPKLE+TFESLVDAFVFLD NKDGYVSKSEM+SAINETTSGE
Sbjct: 121 VYLLKDDPN---HKSQFGMPKLEETFESLVDAFVFLDTNKDGYVSKSEMISAINETTSGE 180

Query: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEE 228
           RSSGRI MRRFEEMDWDKNGMVN KEFLFAFTRWVGIDENEDGEEEE
Sbjct: 181 RSSGRIGMRRFEEMDWDKNGMVNIKEFLFAFTRWVGIDENEDGEEEE 224

[Close]

BLAST of CSPI03G04350 vs. ExPASy TrEMBL
Match: A0A6J1DN80 (probable calcium-binding protein CML21 OS=Momordica charantia OX=3673 GN=LOC111022149 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.4e-109
Identity = 204/228 (89.47%), Postives = 215/228 (94.30%), Query Frame = 0


Query: 1   MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT 60
           MGS VGK ESPR+CVPETKLEAKMVE M QRA+KGSIIRSFD IILKFPKIDDS RNC +
Sbjct: 1   MGSAVGKYESPRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCIS 60

Query: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
            FQQFDED NGIIDRRELKKCFDGLEILLTEEEIDDLF+ACDISTAMG+KFNEFIVLL L
Sbjct: 61  TFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSL 120

Query: 121 VYLLKDDPN-AVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG 180
           VYLL+DDP  AVF+KSQFGMPKLE+TFESLVDAFVFLDKNKDGYVSKSEMVSAI+ETTSG
Sbjct: 121 VYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAIHETTSG 180

Query: 181 ERSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEE 228
           ERSSGRIAM+RFEEMDWDKNGMVNFKEFLFAFTRWVG+ ENEDGEEEE
Sbjct: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGEEEE 228

[Close]

BLAST of CSPI03G04350 vs. NCBI nr
Match: XP_004149571.1 (probable calcium-binding protein CML21 [Cucumis sativus] >KGN56046.1 hypothetical protein Csa_009502 [Cucumis sativus])

HSP 1 Score: 457.2 bits (1175), Expect = 8.3e-125
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 0


Query: 1   MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT 60
           MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT
Sbjct: 1   MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT 60

Query: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
           IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL
Sbjct: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120

Query: 121 VYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE 180
           VYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE
Sbjct: 121 VYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE 180

Query: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEEEE 230
           RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEEEE
Sbjct: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEEEE 229

[Close]

BLAST of CSPI03G04350 vs. NCBI nr
Match: XP_008449066.1 (PREDICTED: probable calcium-binding protein CML21 [Cucumis melo])

HSP 1 Score: 433.3 bits (1113), Expect = 1.3e-117
Identity = 213/224 (95.09%), Postives = 222/224 (99.11%), Query Frame = 0


Query: 1   MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT 60
           MGSVVGKLESPRECVPETKLEAKMVETM++RATKGSIIRSFDCI+LKFPKIDDSLRNCKT
Sbjct: 1   MGSVVGKLESPRECVPETKLEAKMVETMRRRATKGSIIRSFDCILLKFPKIDDSLRNCKT 60

Query: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
           IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLF+ACDIS AMG+KFNEFIVLLCL
Sbjct: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISAAMGIKFNEFIVLLCL 120

Query: 121 VYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE 180
           VYLLKDDP+AVFSKSQFGMPKLE+TFESLVDAFVFLDKNKDGYVSKSEM+SAINETTSGE
Sbjct: 121 VYLLKDDPDAVFSKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMISAINETTSGE 180

Query: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGE 225
           RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENE+ E
Sbjct: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEEEE 224

[Close]

BLAST of CSPI03G04350 vs. NCBI nr
Match: XP_038905190.1 (probable calcium-binding protein CML21 [Benincasa hispida] >XP_038905191.1 probable calcium-binding protein CML21 [Benincasa hispida] >XP_038905192.1 probable calcium-binding protein CML21 [Benincasa hispida] >XP_038905193.1 probable calcium-binding protein CML21 [Benincasa hispida])

HSP 1 Score: 425.6 bits (1093), Expect = 2.7e-115
Identity = 214/229 (93.45%), Postives = 220/229 (96.07%), Query Frame = 0


Query: 1   MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT 60
           MGSVVGKLESP +CVPETKLEAKMVE M+ RA+KGSIIRSFD IILKFPKIDDSLR CKT
Sbjct: 1   MGSVVGKLESP-DCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCKT 60

Query: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
           IFQQFDEDLNGIIDRRELK+CFDGLEI LTEEEIDDLFDACDISTAMGMKFNEFIVLLCL
Sbjct: 61  IFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120

Query: 121 VYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE 180
           VYLLKDDPNA FSKSQFGMPKLE+TFESLVDAF FLDKNKDGYVSKSEM+SAINETTSGE
Sbjct: 121 VYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSGE 180

Query: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEEEE 230
           RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFT WVGIDENEDGEEEEEE
Sbjct: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTHWVGIDENEDGEEEEEE 228

[Close]

BLAST of CSPI03G04350 vs. NCBI nr
Match: XP_023540519.1 (probable calcium-binding protein CML21 [Cucurbita pepo subsp. pepo] >XP_023540520.1 probable calcium-binding protein CML21 [Cucurbita pepo subsp. pepo] >XP_023540521.1 probable calcium-binding protein CML21 [Cucurbita pepo subsp. pepo] >XP_023540522.1 probable calcium-binding protein CML21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 409.5 bits (1051), Expect = 2.0e-110
Identity = 205/228 (89.91%), Postives = 215/228 (94.30%), Query Frame = 0


Query: 1   MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT 60
           MGSVVGKLES  ECVPETKLEAKMVE M+QRA+KGSIIRSFDCIILKFPKID SLR CKT
Sbjct: 1   MGSVVGKLESHSECVPETKLEAKMVEAMRQRASKGSIIRSFDCIILKFPKIDGSLRKCKT 60

Query: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
           IF++FDED NGIIDRRELK+CFDGLEI LT+EEIDDLFDACD+STAMG+KFNEFIVLLCL
Sbjct: 61  IFEKFDEDSNGIIDRRELKECFDGLEISLTQEEIDDLFDACDMSTAMGIKFNEFIVLLCL 120

Query: 121 VYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE 180
           VYLLKDDPN    KSQFGMPKLE+TFESLVDAFVFLD NKDGYVSKSEM+SAINETTSGE
Sbjct: 121 VYLLKDDPN---HKSQFGMPKLEETFESLVDAFVFLDANKDGYVSKSEMISAINETTSGE 180

Query: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEEE 229
           RSSGRI MRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEEE
Sbjct: 181 RSSGRIGMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEEE 225

[Close]

BLAST of CSPI03G04350 vs. NCBI nr
Match: KAG6596750.1 (putative calcium-binding protein CML21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 406.8 bits (1044), Expect = 1.3e-109
Identity = 203/227 (89.43%), Postives = 214/227 (94.27%), Query Frame = 0


Query: 1   MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT 60
           MGSVVGKLES RECVPETKLEAKMVE ++QRA+KGSIIRSFDCIILKFPKID SLR CKT
Sbjct: 1   MGSVVGKLESQRECVPETKLEAKMVEAIRQRASKGSIIRSFDCIILKFPKIDGSLRKCKT 60

Query: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
           IF++FDED NGIIDRRELK+CFDGLEI LT+EEIDDLFDACD+STAMGMKFNEFIVLLCL
Sbjct: 61  IFEKFDEDSNGIIDRRELKECFDGLEISLTQEEIDDLFDACDMSTAMGMKFNEFIVLLCL 120

Query: 121 VYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE 180
           VYLLKDDPN    KSQFGMPKLE+TFESLVDAFVFLD NKDGYVSKSEM+SAINETTSGE
Sbjct: 121 VYLLKDDPN---HKSQFGMPKLEETFESLVDAFVFLDTNKDGYVSKSEMISAINETTSGE 180

Query: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEE 228
           RSSGRI MRRFEEMDWDKNGMVN KEFLFAFTRWVGIDENEDGEEE+
Sbjct: 181 RSSGRIGMRRFEEMDWDKNGMVNIKEFLFAFTRWVGIDENEDGEEEK 224

[Close]

BLAST of CSPI03G04350 vs. TAIR 10
Match: AT4G26470.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 311.6 bits (797), Expect = 5.2e-85
Identity = 158/228 (69.30%), Postives = 186/228 (81.58%), Query Frame = 0


Query: 1   MGSVVGKLES-PRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCK 60
           MG  V K E+  +E VPETKLEAK++E +++RA++G+ ++SF+ I+LKFPKIDD LRNCK
Sbjct: 1   MGGAVTKSETLQKEWVPETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCK 60

Query: 61  TIFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLC 120
            IFQ+FDED NG ID  ELK C   LEI   EEEI+DLF ACDI+  MG+ F EFIVLLC
Sbjct: 61  AIFQEFDEDSNGSIDHTELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLC 120

Query: 121 LVYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG 180
           LVYLLKDD + +  K   GMPKLE TFE+LVD FVFLD+NKDGYVS+ EMV AI+E  SG
Sbjct: 121 LVYLLKDDSSTLQKKWTMGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDE--SG 180

Query: 181 ERSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEE 228
           ERSSGRIAM+RFEEMDWDKNGMVNFKEFLFAFT+WVGIDENE+ EEE+
Sbjct: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTQWVGIDENEEEEEED 226

[Close]

BLAST of CSPI03G04350 vs. TAIR 10
Match: AT4G26470.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 243.0 bits (619), Expect = 2.3e-64
Identity = 126/192 (65.62%), Postives = 151/192 (78.65%), Query Frame = 0


Query: 1   MGSVVGKLES-PRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCK 60
           MG  V K E+  +E VPETKLEAK++E +++RA++G+ ++SF+ I+LKFPKIDD LRNCK
Sbjct: 1   MGGAVTKSETLQKEWVPETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCK 60

Query: 61  TIFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLC 120
            IFQ+FDED NG ID  ELK C   LEI   EEEI+DLF ACDI+  MG+ F EFIVLLC
Sbjct: 61  AIFQEFDEDSNGSIDHTELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLC 120

Query: 121 LVYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG 180
           LVYLLKDD + +  K   GMPKLE TFE+LVD FVFLD+NKDGYVS+ EMV AI+E  SG
Sbjct: 121 LVYLLKDDSSTLQKKWTMGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDE--SG 180

Query: 181 ERSSGRIAMRRF 192
           ERSSGRIAM+RF
Sbjct: 181 ERSSGRIAMKRF 190

[Close]

BLAST of CSPI03G04350 vs. TAIR 10
Match: AT3G24110.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 179.5 bits (454), Expect = 3.1e-45
Identity = 91/208 (43.75%), Postives = 137/208 (65.87%), Query Frame = 0


Query: 17  ETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKTIFQQFDEDLNGIIDRR 76
           + KL  KMVE+        S ++S D II+KFPK+ + LRN +++F+ +D D NG ID  
Sbjct: 18  DAKLARKMVESRSIYPGHRS-LKSMDSIIMKFPKLREGLRNIRSVFESYDNDTNGTIDIE 77

Query: 77  ELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCLVYLLKDDPNAVFSKSQ 136
           ELKKC + L++ L++EE+  L+  CD+  + G++FNEFIVLLCL+YLL    +   ++S+
Sbjct: 78  ELKKCLEELKLSLSDEEVKGLYSWCDVDGSKGIQFNEFIVLLCLIYLLAKPSSESSTESR 137

Query: 137 FGMPKL-EQTFESLVDAFVFLDKNKDGYVSKSEMVSAI-NETTSGERSSGRIAMRRFEEM 196
              PKL E  F+ +V+ F+FLDK+  G ++K++++  + NE    ERS   +   RFEEM
Sbjct: 138 EMGPKLVESIFDPIVEVFLFLDKDGKGKLNKADVIKTLNNEDYPLERSPSHVTNMRFEEM 197

Query: 197 DWDKNGMVNFKEFLFAFTRWVGIDENED 223
           DW + G V F+EFLFAF  WVG+D+ +D
Sbjct: 198 DWGRKGKVGFREFLFAFMSWVGLDDADD 224

[Close]

BLAST of CSPI03G04350 vs. TAIR 10
Match: AT4G04740.1 (calcium-dependent protein kinase 23 )

HSP 1 Score: 63.2 bits (152), Expect = 3.2e-10
Identity = 49/195 (25.13%), Postives = 90/195 (46.15%), Query Frame = 0


Query: 16  PETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKI---DDSLRNCKTIFQQFDEDLNGI 75
           PE  +++ ++  MKQ      + +    + LK   +   ++ ++  KT+F   D + +G 
Sbjct: 333 PEKPIDSTVLSRMKQFRAMNKLKK----LALKVSAVSLSEEEIKGLKTLFANMDTNRSGT 392

Query: 76  IDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCLVYLLKDDPNAVF 135
           I   +L+     L   L+E E+  L +A D+     + + EFI      Y L  D     
Sbjct: 393 ITYEQLQTGLSRLRSRLSETEVQQLVEASDVDGNGTIDYYEFISATMHRYKLHHD----- 452

Query: 136 SKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGERSSGRIAMRRFE 195
                         E +  AF  LDK+K+G++++ E+ SA+ E   G+ +S +  +    
Sbjct: 453 --------------EHVHKAFQHLDKDKNGHITRDELESAMKEYGMGDEASIKEVI---S 501

Query: 196 EMDWDKNGMVNFKEF 208
           E+D D +G +NF+EF
Sbjct: 513 EVDTDNDGKINFEEF 501

[Close]

BLAST of CSPI03G04350 vs. TAIR 10
Match: AT4G21940.1 (calcium-dependent protein kinase 15 )

HSP 1 Score: 62.8 bits (151), Expect = 4.2e-10
Identity = 46/192 (23.96%), Postives = 87/192 (45.31%), Query Frame = 0


Query: 16  PETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKTIFQQFDEDLNGIIDR 75
           P+  +++ ++  MKQ       ++     ++     ++ ++  KT+F   D D +G I  
Sbjct: 366 PDKPIDSAVLSRMKQFRAMNK-LKKLALKVIAESLSEEEIKGLKTMFANMDTDKSGTITY 425

Query: 76  RELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCLVYLLKDDPNAVFSKS 135
            ELK     L   LTE E+  L +A D+     + + EFI             +A   + 
Sbjct: 426 EELKNGLAKLGSKLTEAEVKQLMEAADVDGNGTIDYIEFI-------------SATMHRY 485

Query: 136 QFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGERSSGRIAMRRFEEMD 195
           +F         E +  AF + DK+  G+++  E+ SA+ E   G+ +S +  +    E+D
Sbjct: 486 RFDRD------EHVFKAFQYFDKDNSGFITMDELESAMKEYGMGDEASIKEVI---AEVD 534

Query: 196 WDKNGMVNFKEF 208
            D +G +N++EF
Sbjct: 546 TDNDGRINYEEF 534

The following BLAST results are available for this feature:

BLAST of CSPI03G04350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q52K82	7.4e-84	69.30	Probable calcium-binding protein CML21 OS=Arabidopsis thaliana OX=3702 GN=CML21 ...	[more]
Q9LRN6	4.4e-44	43.75	Probable calcium-binding protein CML22 OS=Arabidopsis thaliana OX=3702 GN=CML22 ...	[more]
Q9M101	4.6e-09	25.13	Calcium-dependent protein kinase 23 OS=Arabidopsis thaliana OX=3702 GN=CPK23 PE=...	[more]
O49717	5.9e-09	23.96	Calcium-dependent protein kinase 15 OS=Arabidopsis thaliana OX=3702 GN=CPK15 PE=...	[more]
Q9ZSA2	1.0e-08	24.48	Calcium-dependent protein kinase 21 OS=Arabidopsis thaliana OX=3702 GN=CPK21 PE=...	[more]

BLAST of CSPI03G04350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L7G7	4.0e-125	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G061000 PE=4 SV=1	[more]
A0A1S3BLU5	6.2e-118	95.09	probable calcium-binding protein CML21 OS=Cucumis melo OX=3656 GN=LOC103491047 P...	[more]
A0A6J1KV85	6.2e-110	89.87	probable calcium-binding protein CML21 OS=Cucurbita maxima OX=3661 GN=LOC1114984...	[more]
A0A6J1G727	8.1e-110	89.43	probable calcium-binding protein CML21 OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1DN80	2.4e-109	89.47	probable calcium-binding protein CML21 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of CSPI03G04350 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149571.1	8.3e-125	100.00	probable calcium-binding protein CML21 [Cucumis sativus] >KGN56046.1 hypothetica...	[more]
XP_008449066.1	1.3e-117	95.09	PREDICTED: probable calcium-binding protein CML21 [Cucumis melo]	[more]
XP_038905190.1	2.7e-115	93.45	probable calcium-binding protein CML21 [Benincasa hispida] >XP_038905191.1 proba...	[more]
XP_023540519.1	2.0e-110	89.91	probable calcium-binding protein CML21 [Cucurbita pepo subsp. pepo] >XP_02354052...	[more]
KAG6596750.1	1.3e-109	89.43	putative calcium-binding protein CML21, partial [Cucurbita argyrosperma subsp. s...	[more]

BLAST of CSPI03G04350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G26470.1	5.2e-85	69.30	Calcium-binding EF-hand family protein	[more]
AT4G26470.2	2.3e-64	65.63	Calcium-binding EF-hand family protein	[more]
AT3G24110.1	3.1e-45	43.75	Calcium-binding EF-hand family protein	[more]
AT4G04740.1	3.2e-10	25.13	calcium-dependent protein kinase 23	[more]
AT4G21940.1	4.2e-10	23.96	calcium-dependent protein kinase 15	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 220..229
None	No IPR available	GENE3D	1.10.238.10		coord: 16..214 e-value: 2.3E-28 score: 100.9
None	No IPR available	PANTHER	PTHR23064	TROPONIN	coord: 1..224
None	No IPR available	PANTHER	PTHR23064:SF23	CALCIUM-BINDING PROTEIN CML21-RELATED	coord: 1..224
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 57..85 e-value: 5.6 score: 11.7 coord: 186..214 e-value: 3.1 score: 13.8 coord: 148..176 e-value: 0.061 score: 22.4
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 146..211 e-value: 6.5E-8 score: 32.9
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 61..80 e-value: 0.015 score: 14.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 53..88 score: 11.472807
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 182..217 score: 9.659605
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 144..179 score: 10.580154
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 149..212 e-value: 1.37998E-7 score: 45.2313
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 157..169
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 54..217

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G04350.1	CSPI03G04350.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006406	mRNA export from nucleus
molecular_function	GO:0005509	calcium ion binding