CSPI03G04010 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G04010
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	caffeic acid 3-O-methyltransferase-like
Location	Chr3: 3320470 .. 3321033 (+)
RNA-Seq Expression	CSPI03G04010
Synteny	CSPI03G04010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCTCCGACAACGACACCGCCGTCGAACAGCAACAACATTATGCTTACGCAGGACACCTCGTTACCTTATCAGTCCTACCCATGACACTCCAAGCAGTGTTCGAGCTTGGTGTGTTCGAGATCTTGGCAAAGGCTGGGGATGGGGCCAACCTCTCTCCAGCAGAGATAGCGGCTGAGATAACCACTACAAACCCCAACGCAGCATTGATGCTTGATCGGATGTTGAGGCTTTTGGCATGCCACTCGGTGGTGGGATGCTCTATAGTTTCTGATAAAGATGGGAATGTGCAAAGGCTTTATAGCCTTACTCCGATTTCTAAATGTTATGTTCGAAATGAAGATGGTGTTTCGTTGGGTCCAATGTTATCATTGATTCAAGATAAAGTGTTTCTGCAAAGCTGGTTTGCTCTCCTTAATTTCTTTATGCTTTTTAATATATATTTTTTTCGTTTAATAGAAGGGAAATTAGTAGGAATAGCGAAGATAAAATATAAATGGAGATATGTTATATTAGTGAGAAATTATGTAAGTACAGCTATGTTTGAGAAATTAGAGAGT

mRNA sequence

ATGGCTTCCTCCGACAACGACACCGCCGTCGAACAGCAACAACATTATGCTTACGCAGGACACCTCGTTACCTTATCAGTCCTACCCATGACACTCCAAGCAGTGTTCGAGCTTGGTGTGTTCGAGATCTTGGCAAAGGCTGGGGATGGGGCCAACCTCTCTCCAGCAGAGATAGCGGCTGAGATAACCACTACAAACCCCAACGCAGCATTGATGCTTGATCGGATGTTGAGGCTTTTGGCATGCCACTCGGTGGTGGGATGCTCTATAGTTTCTGATAAAGATGGGAATGTGCAAAGGCTTTATAGCCTTACTCCGATTTCTAAATGTTATGTTCGAAATGAAGATGGTGTTTCGTTGGGTCCAATGTTATCATTGATTCAAGATAAAGTGTTTCTGCAAAGCTGGTTTGCTCTCCTTAATTTCTTTATGCTTTTTAATATATATTTTTTTCGTTTAATAGAAGGGAAATTAGTAGGAATAGCGAAGATAAAATATAAATGGAGATATGTTATATTAGTGAGAAATTATGTAAGTACAGCTATGTTTGAGAAATTAGAGAGT

Coding sequence (CDS)

ATGGCTTCCTCCGACAACGACACCGCCGTCGAACAGCAACAACATTATGCTTACGCAGGACACCTCGTTACCTTATCAGTCCTACCCATGACACTCCAAGCAGTGTTCGAGCTTGGTGTGTTCGAGATCTTGGCAAAGGCTGGGGATGGGGCCAACCTCTCTCCAGCAGAGATAGCGGCTGAGATAACCACTACAAACCCCAACGCAGCATTGATGCTTGATCGGATGTTGAGGCTTTTGGCATGCCACTCGGTGGTGGGATGCTCTATAGTTTCTGATAAAGATGGGAATGTGCAAAGGCTTTATAGCCTTACTCCGATTTCTAAATGTTATGTTCGAAATGAAGATGGTGTTTCGTTGGGTCCAATGTTATCATTGATTCAAGATAAAGTGTTTCTGCAAAGCTGGTTTGCTCTCCTTAATTTCTTTATGCTTTTTAATATATATTTTTTTCGTTTAATAGAAGGGAAATTAGTAGGAATAGCGAAGATAAAATATAAATGGAGATATGTTATATTAGTGAGAAATTATGTAAGTACAGCTATGTTTGAGAAATTAGAGAGT

Protein sequence

MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGPMLSLIQDKVFLQSWFALLNFFMLFNIYFFRLIEGKLVGIAKIKYKWRYVILVRNYVSTAMFEKLES

Homology

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BLAST of CSPI03G04010 vs. ExPASy Swiss-Prot
Match: Q9FQY8 (Caffeic acid 3-O-methyltransferase OS=Capsicum annuum OX=4072 GN=COMT PE=2 SV=2)

HSP 1 Score: 136.3 bits (342), Expect = 3.5e-31
Identity = 66/139 (47.48%), Postives = 98/139 (70.50%), Query Frame = 0


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           M S++ +    + + + +A  L + SVLPM L++  EL + EI+AKAG GA +SP+E+AA
Sbjct: 1   MDSTNQNLTQTEDEAFLFAMQLASASVLPMVLKSALELDLLEIMAKAGPGAAISPSELAA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           ++ T NP A +MLDRMLRLLA +SV+ C++ +  DG V+RLYSL P+ K   +N DGVS+
Sbjct: 61  QLPTKNPEAPVMLDRMLRLLATYSVLNCTLRTLPDGRVERLYSLAPVCKLLTKNADGVSV 120

Query: 121 GPMLSLIQDKVFLQSWFAL 140
            P+L + QDKV ++SW+ L
Sbjct: 121 APLLLMNQDKVLMESWYHL 139

[Close]

BLAST of CSPI03G04010 vs. ExPASy Swiss-Prot
Match: P28002 (Caffeic acid 3-O-methyltransferase OS=Medicago sativa OX=3879 PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 4.5e-31
Identity = 65/132 (49.24%), Postives = 95/132 (71.97%), Query Frame = 0


Query: 8   TAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNP 67
           T +  ++   +A  L + SVLPM L++  EL + EI+AKAG GA +SP EIA+++ TTNP
Sbjct: 12  THISDEEANLFAMQLASASVLPMILKSALELDLLEIIAKAGPGAQISPIEIASQLPTTNP 71

Query: 68  NAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGPMLSLI 127
           +A +MLDRMLRLLAC+ ++ CS+ + +DG VQRLY L  ++K  V+NEDGVS+  +  + 
Sbjct: 72  DAPVMLDRMLRLLACYIILTCSVRTQQDGKVQRLYGLATVAKYLVKNEDGVSISALNLMN 131

Query: 128 QDKVFLQSWFAL 140
           QDKV ++SW+ L
Sbjct: 132 QDKVLMESWYHL 143

[Close]

BLAST of CSPI03G04010 vs. ExPASy Swiss-Prot
Match: O23760 (Caffeic acid 3-O-methyltransferase OS=Clarkia breweri OX=36903 GN=COMT PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.0e-30
Identity = 71/135 (52.59%), Postives = 94/135 (69.63%), Query Frame = 0


Query: 8   TAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKA---GDGANLSPAEIAAEITT 67
           T V  ++   +A  L + SVLPM L+A  EL V EI+AK+   G GA +SPAEIAA++ T
Sbjct: 14  THVSDEEANLFAMQLASASVLPMVLKAAIELDVLEIMAKSIPHGSGAYISPAEIAAQLPT 73

Query: 68  TNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGPML 127
           TNP+A +MLDR+LRLLA +SVV CS+    DG V+RLY L P+ K   +NEDGVSL P+ 
Sbjct: 74  TNPDAPVMLDRVLRLLASYSVVTCSLRELPDGKVERLYGLAPVCKFLTKNEDGVSLAPLC 133

Query: 128 SLIQDKVFLQSWFAL 140
            + QDKV ++SW+ L
Sbjct: 134 LMNQDKVLMESWYYL 148

[Close]

BLAST of CSPI03G04010 vs. ExPASy Swiss-Prot
Match: O81646 (Caffeic acid 3-O-methyltransferase OS=Capsicum chinense OX=80379 GN=COMT PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 3.8e-30
Identity = 65/139 (46.76%), Postives = 95/139 (68.35%), Query Frame = 0


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           M S +      + + + +A  L + SVLPM L+A  EL + EI+AK+G GA +SP+E+AA
Sbjct: 1   MGSINQSLTQTEDEAFVFAMQLASASVLPMVLKATVELDLLEIMAKSGPGAFISPSELAA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           ++ T NP A +MLDRM RLLA +SV+ C++ +  DG V+RLYSL P+ K   +N DGVS+
Sbjct: 61  QLPTKNPEAPVMLDRMFRLLATYSVLNCTLRTLPDGRVERLYSLAPVCKFLTKNGDGVSI 120

Query: 121 GPMLSLIQDKVFLQSWFAL 140
            P+L + QDKV ++SW+ L
Sbjct: 121 APILLMNQDKVLMESWYHL 139

[Close]

BLAST of CSPI03G04010 vs. ExPASy Swiss-Prot
Match: Q00763 (Caffeic acid 3-O-methyltransferase 1 OS=Populus tremuloides OX=3693 GN=OMT1 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 4.2e-29
Identity = 64/132 (48.48%), Postives = 91/132 (68.94%), Query Frame = 0


Query: 8   TAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNP 67
           T V  ++ + +A  L + SVLPM L+   EL + EI+AKAG GA LS +EIA+ + T NP
Sbjct: 12  TQVSDEEAHLFAMQLASASVLPMILKTAIELDLLEIMAKAGPGAFLSTSEIASHLPTKNP 71

Query: 68  NAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGPMLSLI 127
           +A +MLDR+LRLLA +S++ CS+    DG V+RLY L P+ K   +NEDGVS+ P+  + 
Sbjct: 72  DAPVMLDRILRLLASYSILTCSLKDLPDGKVERLYGLAPVCKFLTKNEDGVSVSPLCLMN 131

Query: 128 QDKVFLQSWFAL 140
           QDKV ++SW+ L
Sbjct: 132 QDKVLMESWYYL 143

[Close]

BLAST of CSPI03G04010 vs. ExPASy TrEMBL
Match: A0A1S3BM08 (caffeic acid 3-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103491014 PE=3 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 1.1e-53
Identity = 120/141 (85.11%), Postives = 124/141 (87.94%), Query Frame = 0


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASSDNDTAVE QQ+YA A  LVTLSVLPMTLQAVFELGVFEILAKAGD   LSPAEI A
Sbjct: 1   MASSDNDTAVE-QQNYANAAELVTLSVLPMTLQAVFELGVFEILAKAGD-RELSPAEITA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           EITTTNP AA M+DRMLRLLA HSVVGCSI SD+DGNVQR YSLTPISK YVRNEDGVSL
Sbjct: 61  EITTTNPYAASMIDRMLRLLASHSVVGCSIASDEDGNVQRFYSLTPISKYYVRNEDGVSL 120

Query: 121 GPMLSLIQDKVFLQSWFALLN 142
           GP+LSLIQDKVFLQSW  L N
Sbjct: 121 GPILSLIQDKVFLQSWSELKN 139

[Close]

BLAST of CSPI03G04010 vs. ExPASy TrEMBL
Match: A0A1S4DY16 (caffeic acid 3-O-methyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC107990972 PE=3 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 2.5e-48
Identity = 99/136 (72.79%), Postives = 117/136 (86.03%), Query Frame = 0


Query: 3   SSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEI 62
           +S +D  V +QQHYAYA  L TLSV+PMTLQ  FELGVFEIL+KAG+GA LS  EIAA I
Sbjct: 43  TSFDDHDVVEQQHYAYAMELATLSVVPMTLQTAFELGVFEILSKAGNGAKLSSTEIAANI 102

Query: 63  TTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGP 122
           TTTNP A+LM+DRMLRLLA H+VVGCS+ SD+DGNV+RLY+LT +SKCYVRNEDGVSLGP
Sbjct: 103 TTTNPEASLMIDRMLRLLASHAVVGCSLASDEDGNVKRLYNLTSVSKCYVRNEDGVSLGP 162

Query: 123 MLSLIQDKVFLQSWFA 139
           +L+L+QDKV L +WFA
Sbjct: 163 LLTLLQDKVLLHTWFA 178

[Close]

BLAST of CSPI03G04010 vs. ExPASy TrEMBL
Match: A0A6J1FGP5 (caffeic acid 3-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111443858 PE=3 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 4.2e-48
Identity = 99/141 (70.21%), Postives = 123/141 (87.23%), Query Frame = 0


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASS++D + ++QQHY+YA  LVTLSVLPMTLQA FELG+FEILAKAGDGA LSP EIAA
Sbjct: 11  MASSNSDDS-QEQQHYSYAAQLVTLSVLPMTLQAAFELGLFEILAKAGDGAELSPTEIAA 70

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           +ITT+NP+AA M+DR+LRL+A HSVVGCS++ D++GN QRLYSL P++K ++ NEDGVSL
Sbjct: 71  KITTSNPDAASMIDRILRLMAAHSVVGCSVIIDENGNQQRLYSLMPVTKYFIPNEDGVSL 130

Query: 121 GPMLSLIQDKVFLQSWFALLN 142
           GP++S+IQDKVFL SW  L N
Sbjct: 131 GPIVSMIQDKVFLHSWSELKN 150

[Close]

BLAST of CSPI03G04010 vs. ExPASy TrEMBL
Match: A0A6J1FG58 (caffeic acid 3-O-methyltransferase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443875 PE=3 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 4.2e-48
Identity = 99/141 (70.21%), Postives = 123/141 (87.23%), Query Frame = 0


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASS++D + ++QQHY+YA  LVTLSVLPMTLQA FELG+FEILAKAGDGA LSP EIAA
Sbjct: 11  MASSNSDDS-QEQQHYSYAAQLVTLSVLPMTLQAAFELGLFEILAKAGDGAELSPTEIAA 70

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           +ITT+NP+AA M+DR+LRL+A HSVVGCS++ D++GN QRLYSL P++K ++ NEDGVSL
Sbjct: 71  KITTSNPDAASMIDRILRLMAAHSVVGCSVIIDENGNQQRLYSLMPVTKYFIPNEDGVSL 130

Query: 121 GPMLSLIQDKVFLQSWFALLN 142
           GP++S+IQDKVFL SW  L N
Sbjct: 131 GPIVSMIQDKVFLHSWSELKN 150

[Close]

BLAST of CSPI03G04010 vs. ExPASy TrEMBL
Match: A0A6J1FAK8 (caffeic acid 3-O-methyltransferase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443906 PE=3 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 2.1e-47
Identity = 99/141 (70.21%), Postives = 121/141 (85.82%), Query Frame = 0


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASS++D   ++QQHYAYA  LVT +VLPMTLQA FELG+FEILAKAGDGA LSP EIAA
Sbjct: 11  MASSNSDDG-QEQQHYAYAAQLVTSTVLPMTLQAAFELGLFEILAKAGDGAELSPTEIAA 70

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           +ITT+NP+AA M+DR+LRLLA H+VVGCS++ D++GN QRLYS+TP++K +V NEDGVSL
Sbjct: 71  KITTSNPDAASMIDRILRLLAAHTVVGCSVIIDENGNQQRLYSITPVAKYFVLNEDGVSL 130

Query: 121 GPMLSLIQDKVFLQSWFALLN 142
           GP+LS+IQDKV L SW  L N
Sbjct: 131 GPILSMIQDKVILHSWSELKN 150

[Close]

BLAST of CSPI03G04010 vs. NCBI nr
Match: XP_004150935.3 (caffeic acid 3-O-methyltransferase [Cucumis sativus] >KAE8650102.1 hypothetical protein Csa_010461 [Cucumis sativus])

HSP 1 Score: 270.8 bits (691), Expect = 9.0e-69
Identity = 138/141 (97.87%), Postives = 138/141 (97.87%), Query Frame = 0


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA
Sbjct: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL
Sbjct: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120

Query: 121 GPMLSLIQDKVFLQSWFALLN 142
           GPMLSLIQDKVFLQSW  L N
Sbjct: 121 GPMLSLIQDKVFLQSWSELKN 141

[Close]

BLAST of CSPI03G04010 vs. NCBI nr
Match: KAE8650103.1 (hypothetical protein Csa_010827 [Cucumis sativus])

HSP 1 Score: 250.8 bits (639), Expect = 9.6e-63
Identity = 130/141 (92.20%), Postives = 133/141 (94.33%), Query Frame = 0


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASSDN+TAVEQQQ+YAYA  LVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA
Sbjct: 1   MASSDNNTAVEQQQNYAYAAQLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           EITTTNP+AALMLDRMLRLLACHSVVGCSI SDKDGNVQRLYSLTPISK YVRNEDGVSL
Sbjct: 61  EITTTNPDAALMLDRMLRLLACHSVVGCSIASDKDGNVQRLYSLTPISKYYVRNEDGVSL 120

Query: 121 GPMLSLIQDKVFLQSWFALLN 142
           GP LSLIQDKVFLQSW  L N
Sbjct: 121 GPTLSLIQDKVFLQSWSELKN 141

[Close]

BLAST of CSPI03G04010 vs. NCBI nr
Match: XP_031738579.1 (LOW QUALITY PROTEIN: caffeic acid 3-O-methyltransferase [Cucumis sativus])

HSP 1 Score: 250.8 bits (639), Expect = 9.6e-63
Identity = 130/141 (92.20%), Postives = 133/141 (94.33%), Query Frame = 0


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASSDN+TAVEQQQ+YAYA  LVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA
Sbjct: 1   MASSDNNTAVEQQQNYAYAAQLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           EITTTNP+AALMLDRMLRLLACHSVVGCSI SDKDGNVQRLYSLTPISK YVRNEDGVSL
Sbjct: 61  EITTTNPDAALMLDRMLRLLACHSVVGCSIASDKDGNVQRLYSLTPISKYYVRNEDGVSL 120

Query: 121 GPMLSLIQDKVFLQSWFALLN 142
           GP LSLIQDKVFLQSW  L N
Sbjct: 121 GPTLSLIQDKVFLQSWSELKN 141

[Close]

BLAST of CSPI03G04010 vs. NCBI nr
Match: XP_008449018.1 (PREDICTED: caffeic acid 3-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 219.5 bits (558), Expect = 2.4e-53
Identity = 120/141 (85.11%), Postives = 124/141 (87.94%), Query Frame = 0


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASSDNDTAVE QQ+YA A  LVTLSVLPMTLQAVFELGVFEILAKAGD   LSPAEI A
Sbjct: 1   MASSDNDTAVE-QQNYANAAELVTLSVLPMTLQAVFELGVFEILAKAGD-RELSPAEITA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           EITTTNP AA M+DRMLRLLA HSVVGCSI SD+DGNVQR YSLTPISK YVRNEDGVSL
Sbjct: 61  EITTTNPYAASMIDRMLRLLASHSVVGCSIASDEDGNVQRFYSLTPISKYYVRNEDGVSL 120

Query: 121 GPMLSLIQDKVFLQSWFALLN 142
           GP+LSLIQDKVFLQSW  L N
Sbjct: 121 GPILSLIQDKVFLQSWSELKN 139

[Close]

BLAST of CSPI03G04010 vs. NCBI nr
Match: XP_038903769.1 (caffeic acid 3-O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 210.7 bits (535), Expect = 1.1e-50
Identity = 112/139 (80.58%), Postives = 120/139 (86.33%), Query Frame = 0


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           + SSDND AV +QQHYAYA  LVTLSVLPMTLQAVFELGVFEILAKAGDGA LSPA+IAA
Sbjct: 52  ITSSDND-AVLEQQHYAYAAQLVTLSVLPMTLQAVFELGVFEILAKAGDGAELSPADIAA 111

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           EITT NP+AALM+DRML LLA HSVVG S+  D+DG  QRLYSLTP+SK YVRNEDGVSL
Sbjct: 112 EITTKNPDAALMIDRMLWLLASHSVVGFSVGFDEDGKTQRLYSLTPVSKYYVRNEDGVSL 171

Query: 121 GPMLSLIQDKVFLQSWFAL 140
           GP LSLIQDKVFL SW  L
Sbjct: 172 GPTLSLIQDKVFLHSWSEL 189

[Close]

BLAST of CSPI03G04010 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 104.0 bits (258), Expect = 1.4e-22
Identity = 60/149 (40.27%), Postives = 91/149 (61.07%), Query Frame = 0


Query: 18  YAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNPNAALMLDRML 77
           +A  L + SVLPM L++  EL + EI+AK  +G+ +SP EIA+++ T NP A +MLDR+L
Sbjct: 22  FAMQLASASVLPMALKSALELDLLEIMAK--NGSPMSPTEIASKLPTKNPEAPVMLDRIL 81

Query: 78  RLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGPMLSLIQDKVFLQSWF 137
           RLL  +SV+ CS        V+R+Y L P+ K   +NEDGVS+  +  + QDKV ++SW+
Sbjct: 82  RLLTSYSVLTCSNRKLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWY 141

Query: 138 ALLNFFMLFNIYFFRLIEGKLVGIAKIKY 167
            L +  +   I F      K  G++  +Y
Sbjct: 142 HLKDAILDGGIPF-----NKAYGMSAFEY 163

[Close]

BLAST of CSPI03G04010 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 96.3 bits (238), Expect = 2.8e-20
Identity = 51/134 (38.06%), Postives = 81/134 (60.45%), Query Frame = 0


Query: 10  VEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITT--TNP 69
           V+++     A  +V     PM L+A FELGV + +A AG+   LSP EIA  + T  TNP
Sbjct: 24  VDEKMVSLQAESIVNTVAFPMVLKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKPTNP 83

Query: 70  NAALMLDRMLRLLACHSVVGCSIV----SDKDGNVQRLYSLTPISKCYVRNEDGV-SLGP 129
            A ++LDRML LL  HS++ C ++    + + G ++R+Y+  P+ K ++R+ DG  SL P
Sbjct: 84  EAPVLLDRMLSLLVSHSILKCRMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGSLVP 143

Query: 130 MLSLIQDKVFLQSW 137
           +  L+  +VF ++W
Sbjct: 144 LFMLLHTQVFFKTW 157

[Close]

BLAST of CSPI03G04010 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 93.6 bits (231), Expect = 1.8e-19
Identity = 50/134 (37.31%), Postives = 81/134 (60.45%), Query Frame = 0


Query: 10  VEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITT--TNP 69
           ++++     A  +V     PM L+A  ELGV + +A A +G  LSP+EIA  +    TNP
Sbjct: 24  IDEKMVSLQAESIVNAVAFPMVLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNKPTNP 83

Query: 70  NAALMLDRMLRLLACHSVVGCSIVSDKD----GNVQRLYSLTPISKCYVRNEDGV-SLGP 129
            A ++LDRMLRLL  HS++ C +V  ++    G ++R+Y+  PI K ++++ DG  SL  
Sbjct: 84  EAPVLLDRMLRLLVSHSILKCCMVESRENGQTGKIERVYAAEPICKYFLKDSDGSGSLSS 143

Query: 130 MLSLIQDKVFLQSW 137
           +L L+  +V L++W
Sbjct: 144 LLLLLHSQVILKTW 157

[Close]

BLAST of CSPI03G04010 vs. TAIR 10
Match: AT1G33030.1 (O-methyltransferase family protein )

HSP 1 Score: 82.4 bits (202), Expect = 4.2e-16
Identity = 48/131 (36.64%), Postives = 82/131 (62.60%), Query Frame = 0


Query: 10  VEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNPNA 69
           +E+Q   +YA  L + SVLPM L+   +LG+F+ILA++G  +      + +  T  + ++
Sbjct: 1   MEEQNLSSYAMILSSSSVLPMVLKTAIDLGLFDILAESGPSSASQIFSLLSNETKKHHDS 60

Query: 70  ALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNED-GVSLGPMLSLIQ 129
           +L ++R+LR LA +S++ CS VS + G    +Y L P++K + +N++ G SL PM++L Q
Sbjct: 61  SL-VNRILRFLASYSILTCS-VSTEHGEPFAIYGLAPVAKYFTKNQNGGGSLAPMVNLFQ 120

Query: 130 DKVFLQSWFAL 140
           DKV    W+ L
Sbjct: 121 DKVVTDMWYNL 129

[Close]

BLAST of CSPI03G04010 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 80.9 bits (198), Expect = 1.2e-15
Identity = 43/123 (34.96%), Postives = 69/123 (56.10%), Query Frame = 0


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGD-GANLSPAEIA 60
           M S        +++    A  L  L+ +P  ++   EL +FEI+AKA   G+ LSP ++A
Sbjct: 1   MISLQTSGGSSEEEDMLLAIQLGGLNFVPYIVKTARELDLFEIMAKARPLGSYLSPVDLA 60

Query: 61  AEITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVS 120
           +     NP+A +M+DR+LR L  +SV  C +V D++G   R Y L  + K  +++EDG S
Sbjct: 61  SMAAPKNPHAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVGKKLIKDEDGFS 120

Query: 121 LGP 123
           + P
Sbjct: 121 IAP 123

The following BLAST results are available for this feature:

BLAST of CSPI03G04010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FQY8	3.5e-31	47.48	Caffeic acid 3-O-methyltransferase OS=Capsicum annuum OX=4072 GN=COMT PE=2 SV=2	[more]
P28002	4.5e-31	49.24	Caffeic acid 3-O-methyltransferase OS=Medicago sativa OX=3879 PE=1 SV=1	[more]
O23760	1.0e-30	52.59	Caffeic acid 3-O-methyltransferase OS=Clarkia breweri OX=36903 GN=COMT PE=1 SV=1	[more]
O81646	3.8e-30	46.76	Caffeic acid 3-O-methyltransferase OS=Capsicum chinense OX=80379 GN=COMT PE=2 SV...	[more]
Q00763	4.2e-29	48.48	Caffeic acid 3-O-methyltransferase 1 OS=Populus tremuloides OX=3693 GN=OMT1 PE=1...	[more]

BLAST of CSPI03G04010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BM08	1.1e-53	85.11	caffeic acid 3-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103491014 ...	[more]
A0A1S4DY16	2.5e-48	72.79	caffeic acid 3-O-methyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC10799097...	[more]
A0A6J1FGP5	4.2e-48	70.21	caffeic acid 3-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1FG58	4.2e-48	70.21	caffeic acid 3-O-methyltransferase-like isoform X2 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1FAK8	2.1e-47	70.21	caffeic acid 3-O-methyltransferase-like isoform X2 OS=Cucurbita moschata OX=3662...	[more]

BLAST of CSPI03G04010 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004150935.3	9.0e-69	97.87	caffeic acid 3-O-methyltransferase [Cucumis sativus] >KAE8650102.1 hypothetical ...	[more]
KAE8650103.1	9.6e-63	92.20	hypothetical protein Csa_010827 [Cucumis sativus]	[more]
XP_031738579.1	9.6e-63	92.20	LOW QUALITY PROTEIN: caffeic acid 3-O-methyltransferase [Cucumis sativus]	[more]
XP_008449018.1	2.4e-53	85.11	PREDICTED: caffeic acid 3-O-methyltransferase-like [Cucumis melo]	[more]
XP_038903769.1	1.1e-50	80.58	caffeic acid 3-O-methyltransferase-like [Benincasa hispida]	[more]

BLAST of CSPI03G04010 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54160.1	1.4e-22	40.27	O-methyltransferase 1	[more]
AT1G77520.1	2.8e-20	38.06	O-methyltransferase family protein	[more]
AT1G77530.1	1.8e-19	37.31	O-methyltransferase family protein	[more]
AT1G33030.1	4.2e-16	36.64	O-methyltransferase family protein	[more]
AT1G51990.1	1.2e-15	34.96	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 30..81 e-value: 3.8E-18 score: 65.2
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 26..145 e-value: 4.5E-44 score: 151.5
None	No IPR available	PANTHER	PTHR11746:SF220	CAFFEIC ACID 3-O-METHYLTRANSFERASE 1-LIKE	coord: 15..139
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 15..139
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 11..114

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G04010.1	CSPI03G04010.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity