CSPI03G03830 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G03830
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	subtilisin-like protease SBT5.3
Location	Chr3: 3155539 .. 3156060 (+)
RNA-Seq Expression	CSPI03G03830
Synteny	CSPI03G03830

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGTACAATATCAGACAACGTCAAGCCCAAGGCAACCCCATTTGATTATGGTGCAGGACATGTCCATCCCAATAGTGCAATGGACCCCGGCCTCGTTTACGACACAACCATCGACGATTACTTGAACTTCTTATGTGCACGTGGCTACAACTCCCTCACTCTCAAGAATTTCTATAACAAACCATTTGTCTGCGCAAAATCGTTCACATTGACCGATCTCAACTACCCATCAATCTCGATCCCAAAGTTGCAATTTGGTGCTCCAGTAACGGTCAATAGAAGAGTTAAGAATGTGGGAACCCCAGGAACATACGTAGCACGTGTGAATGCGTCCTCAAAGATCTTAGTTACGGTCGAGCCGAGTACGTTGCAGTTTAATAGTGTGGGTGAAGAGAAGGCTTTCAAGGTTGTATTTGAATACAAAGGAAATGAACAAGATAAAGGCTATGTGTTTGGGACATTGATATGGTCAGATGGGAAGCATAATGTTAGAAGTCCTATTGTAGTGAATTTGGGATGA

mRNA sequence

ATGCGTACAATATCAGACAACGTCAAGCCCAAGGCAACCCCATTTGATTATGGTGCAGGACATGTCCATCCCAATAGTGCAATGGACCCCGGCCTCGTTTACGACACAACCATCGACGATTACTTGAACTTCTTATGTGCACGTGGCTACAACTCCCTCACTCTCAAGAATTTCTATAACAAACCATTTGTCTGCGCAAAATCGTTCACATTGACCGATCTCAACTACCCATCAATCTCGATCCCAAAGTTGCAATTTGGTGCTCCAGTAACGGTCAATAGAAGAGTTAAGAATGTGGGAACCCCAGGAACATACGTAGCACGTGTGAATGCGTCCTCAAAGATCTTAGTTACGGTCGAGCCGAGTACGTTGCAGTTTAATAGTGTGGGTGAAGAGAAGGCTTTCAAGGTTGTATTTGAATACAAAGGAAATGAACAAGATAAAGGCTATGTGTTTGGGACATTGATATGGTCAGATGGGAAGCATAATGTTAGAAGTCCTATTGTAGTGAATTTGGGATGA

Coding sequence (CDS)

ATGCGTACAATATCAGACAACGTCAAGCCCAAGGCAACCCCATTTGATTATGGTGCAGGACATGTCCATCCCAATAGTGCAATGGACCCCGGCCTCGTTTACGACACAACCATCGACGATTACTTGAACTTCTTATGTGCACGTGGCTACAACTCCCTCACTCTCAAGAATTTCTATAACAAACCATTTGTCTGCGCAAAATCGTTCACATTGACCGATCTCAACTACCCATCAATCTCGATCCCAAAGTTGCAATTTGGTGCTCCAGTAACGGTCAATAGAAGAGTTAAGAATGTGGGAACCCCAGGAACATACGTAGCACGTGTGAATGCGTCCTCAAAGATCTTAGTTACGGTCGAGCCGAGTACGTTGCAGTTTAATAGTGTGGGTGAAGAGAAGGCTTTCAAGGTTGTATTTGAATACAAAGGAAATGAACAAGATAAAGGCTATGTGTTTGGGACATTGATATGGTCAGATGGGAAGCATAATGTTAGAAGTCCTATTGTAGTGAATTTGGGATGA

Protein sequence

MRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYNKPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG*

Homology

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BLAST of CSPI03G03830 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 3.6e-43
Identity = 88/170 (51.76%), Postives = 110/170 (64.71%), Query Frame = 0


Query: 4   ISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYNKPF 63
           I +    KATPF +GAGHV PN A++PGLVYD  I DYLNFLC+ GYN+  +  F    F
Sbjct: 604 IQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNF 663

Query: 64  VCAK-SFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPS 123
            C+    +L +LNYPSI++P L   + VTV+R VKNVG P  Y  +VN    + V V+P+
Sbjct: 664 TCSSPKISLVNLNYPSITVPNLT-SSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPT 723

Query: 124 TLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNL 173
           +L F  VGE+K FKV+         KGYVFG L+WSD KH VRSPIVV L
Sbjct: 724 SLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

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BLAST of CSPI03G03830 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.1e-36
Identity = 81/163 (49.69%), Postives = 98/163 (60.12%), Query Frame = 0


Query: 11  KATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYNKP-FVCAKSF 70
           KA PF YG+GHV PN A  PGLVYD T  DYL+FLCA GYN+  ++ F   P + C +  
Sbjct: 617 KANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGA 676

Query: 71  TLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSV 130
            L D NYPSI++P L     +TV R++KNVG P TY AR      + V+VEP  L FN  
Sbjct: 677 NLLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKT 736

Query: 131 GEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNL 173
           GE K F++           GYVFG L W+D  H VRSPIVV L
Sbjct: 737 GEVKIFQMTLR-PLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

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BLAST of CSPI03G03830 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 153.7 bits (387), Expect = 1.9e-36
Identity = 75/163 (46.01%), Postives = 102/163 (62.58%), Query Frame = 0


Query: 9   KPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNF-YNKPFVCAK 68
           K  A  F YG+GHV P+ A++PGLVYD ++ DYLNFLCA GY+   +    +N+ F+C+ 
Sbjct: 625 KTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQLISALNFNRTFICSG 684

Query: 69  SFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFN 128
           S ++ DLNYPSI++P L+   PVT+ R V NVG P TY     + +   + V P +L F 
Sbjct: 685 SHSVNDLNYPSITLPNLRL-KPVTIARTVTNVGPPSTYTVSTRSPNGYSIAVVPPSLTFT 744

Query: 129 SVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVV 171
            +GE K FKV+ +       + Y FG L W+DGKH VRSPI V
Sbjct: 745 KIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVRSPITV 786

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BLAST of CSPI03G03830 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 6.0e-30
Identity = 65/163 (39.88%), Postives = 101/163 (61.96%), Query Frame = 0


Query: 12  ATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYNKPFVC--AKSF 71
           +TPFD+GAGHV P +A +PGL+YD T +DYL FLCA  Y S  +++   + + C  +KS+
Sbjct: 594 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 653

Query: 72  TLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNA-SSKILVTVEPSTLQFNS 131
           ++ DLNYPS ++     GA     R V +VG  GTY  +V + ++ + ++VEP+ L F  
Sbjct: 654 SVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKE 713

Query: 132 VGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVN 172
             E+K++ V F    ++      FG++ WSDGKH V SP+ ++
Sbjct: 714 ANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

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BLAST of CSPI03G03830 vs. ExPASy Swiss-Prot
Match: O23357 (Subtilisin-like protease SBT4.2 OS=Arabidopsis thaliana OX=3702 GN=SBT4.2 PE=3 SV=3)

HSP 1 Score: 127.5 bits (319), Expect = 1.5e-28
Identity = 69/160 (43.12%), Postives = 96/160 (60.00%), Query Frame = 0


Query: 15  FDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYNKPFVCAK--SFTLT 74
           F YG+G V+P  A+DPGLVY+   +DYLN LC+  Y+S  +       F C++    T+ 
Sbjct: 564 FAYGSGFVNPTVAVDPGLVYEIAKEDYLNMLCSLDYSSQGISTIAGGTFTCSEQSKLTMR 623

Query: 75  DLNYPSISIP-KLQFGAPVTVNRRVKNVGTPG-TYVARVNASSKILVTVEPSTLQFNSVG 134
           +LNYPS+S        + +T +R V NVG  G TY A+++ + K+ + VEP+TL F + G
Sbjct: 624 NLNYPSMSAKVSASSSSDITFSRTVTNVGEKGSTYKAKLSGNPKLSIKVEPATLSFKAPG 683

Query: 135 EEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVV 171
           E+K+F V    K        V  +LIWSDG HNVRSPIVV
Sbjct: 684 EKKSFTVTVSGKSLAGISNIVSASLIWSDGSHNVRSPIVV 723

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BLAST of CSPI03G03830 vs. ExPASy TrEMBL
Match: A0A0A0L601 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045060 PE=3 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 5.6e-95
Identity = 172/173 (99.42%), Postives = 172/173 (99.42%), Query Frame = 0


Query: 1   MRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYN 60
           MRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLT KNFYN
Sbjct: 574 MRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYN 633

Query: 61  KPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVE 120
           KPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVE
Sbjct: 634 KPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVE 693

Query: 121 PSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG 174
           PSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG
Sbjct: 694 PSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG 746

[Close]

BLAST of CSPI03G03830 vs. ExPASy TrEMBL
Match: A0A1S3BLG8 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491134 PE=3 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 2.1e-78
Identity = 148/174 (85.06%), Postives = 158/174 (90.80%), Query Frame = 0


Query: 1   MRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYN 60
           M T+SD VK KATPFDYGAGHV+PN+AMDPGLVYDTTIDDYLNFLCARGYN+ TL +F++
Sbjct: 598 MHTLSDTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHS 657

Query: 61  KPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVE 120
           KPFVCA  FTLTDLNYPSISIP+L+ GAPVTVNRRVKNVGTPGTYVARV ASSKI VTVE
Sbjct: 658 KPFVCATPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVE 717

Query: 121 PSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSD-GKHNVRSPIVVNLG 174
           PSTLQFNSVGEEKAFKV+FEYKG  Q K YVFGTLIWSD GKHNVRSPIVV LG
Sbjct: 718 PSTLQFNSVGEEKAFKVLFEYKGTGQGKSYVFGTLIWSDNGKHNVRSPIVVKLG 771

[Close]

BLAST of CSPI03G03830 vs. ExPASy TrEMBL
Match: A0A5D3D732 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00170 PE=3 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 2.9e-75
Identity = 136/173 (78.61%), Postives = 153/173 (88.44%), Query Frame = 0


Query: 1    MRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYN 60
            M++I D  K KATPF YGAGHVHPN+A+DPGLVYDTTI+DY+NF+CA+GYNS TLK FYN
Sbjct: 1310 MKSILDYNKAKATPFQYGAGHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKRFYN 1369

Query: 61   KPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVE 120
            KP++C KSF LTDLNYPSIS+PKL  G PVT+NRR+KNVGTPGTYVARV  SSK+ VTV+
Sbjct: 1370 KPYLCPKSFPLTDLNYPSISVPKLTIGVPVTINRRLKNVGTPGTYVARVKVSSKVSVTVK 1429

Query: 121  PSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG 174
            PSTLQFNSVGEEKAFKVVFEYKG  Q KG+VFGTLIWSDGKH VRSP+VV LG
Sbjct: 1430 PSTLQFNSVGEEKAFKVVFEYKGKGQGKGHVFGTLIWSDGKHFVRSPMVVKLG 1482

[Close]

BLAST of CSPI03G03830 vs. ExPASy TrEMBL
Match: A0A6J1KUL8 (uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 1.2e-68
Identity = 131/170 (77.06%), Postives = 140/170 (82.35%), Query Frame = 0


Query: 3   TISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYNKP 62
           T+ D  K KATPFDYGAGHVHPN AMDPGLVYDTTIDDYLNFLC +GYNSLTLK F NKP
Sbjct: 591 TMLDFNKVKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKP 650

Query: 63  FVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPS 122
           FVCAK+F  TDLNYPSIS+PKLQ G PVTVNRRVKNVG+ GTYVARV     I V VEPS
Sbjct: 651 FVCAKNFASTDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPS 710

Query: 123 TLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNL 173
           TLQF+SVGEEKAFK+VF Y    +  GYVFG L+WSDGKH VRSPI VNL
Sbjct: 711 TLQFHSVGEEKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNL 760

[Close]

BLAST of CSPI03G03830 vs. ExPASy TrEMBL
Match: A0A6J1GDR2 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453063 PE=3 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 3.8e-67
Identity = 128/170 (75.29%), Postives = 140/170 (82.35%), Query Frame = 0


Query: 3   TISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYNKP 62
           T+ D  K KATPFDYGAG VHPN+AMDPGLVYDTTIDDYLNFLC +GYNSLTLK F NKP
Sbjct: 596 TMLDFNKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKP 655

Query: 63  FVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPS 122
           FVC+K+F +TDLNYPSIS+PKLQ GAPVTVNRRVKNVG+ GTYVARV     I V VEPS
Sbjct: 656 FVCSKNFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPS 715

Query: 123 TLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNL 173
           TLQF+ VGEEK FK+VF Y    +  GYVFG L+WSDGKH VRSPI VNL
Sbjct: 716 TLQFHGVGEEKPFKLVFHYAQQVRRPGYVFGALVWSDGKHFVRSPIAVNL 765

[Close]

BLAST of CSPI03G03830 vs. NCBI nr
Match: XP_031738719.1 (uncharacterized protein LOC101212014 [Cucumis sativus])

HSP 1 Score: 356.7 bits (914), Expect = 1.1e-94
Identity = 172/173 (99.42%), Postives = 172/173 (99.42%), Query Frame = 0


Query: 1   MRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYN 60
           MRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLT KNFYN
Sbjct: 623 MRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYN 682

Query: 61  KPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVE 120
           KPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVE
Sbjct: 683 KPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVE 742

Query: 121 PSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG 174
           PSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG
Sbjct: 743 PSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG 795

[Close]

BLAST of CSPI03G03830 vs. NCBI nr
Match: KAE8650093.1 (hypothetical protein Csa_009608 [Cucumis sativus])

HSP 1 Score: 356.7 bits (914), Expect = 1.1e-94
Identity = 172/173 (99.42%), Postives = 172/173 (99.42%), Query Frame = 0


Query: 1   MRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYN 60
           MRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLT KNFYN
Sbjct: 651 MRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYN 710

Query: 61  KPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVE 120
           KPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVE
Sbjct: 711 KPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVE 770

Query: 121 PSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG 174
           PSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG
Sbjct: 771 PSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG 823

[Close]

BLAST of CSPI03G03830 vs. NCBI nr
Match: XP_038905686.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])

HSP 1 Score: 306.6 bits (784), Expect = 1.4e-79
Identity = 146/172 (84.88%), Postives = 156/172 (90.70%), Query Frame = 0


Query: 2   RTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYNK 61
           RTISD  K KATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNS+ LK FYNK
Sbjct: 595 RTISDTAKMKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSVALKKFYNK 654

Query: 62  PFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEP 121
           PF+CAKSF +TDLNYPSIS+P+L+ GAPVTVNRRVKNVGTPGTYVARV AS  I V+VEP
Sbjct: 655 PFICAKSFAITDLNYPSISVPELRIGAPVTVNRRVKNVGTPGTYVARVKASPAISVSVEP 714

Query: 122 STLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG 174
           STLQFNSVGEEKAFKVVF+YKG EQ +G+VFGTLIWSDGKH VRSPI V LG
Sbjct: 715 STLQFNSVGEEKAFKVVFQYKGKEQRQGHVFGTLIWSDGKHFVRSPIAVKLG 766

[Close]

BLAST of CSPI03G03830 vs. NCBI nr
Match: XP_008449184.1 (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo])

HSP 1 Score: 301.6 bits (771), Expect = 4.4e-78
Identity = 148/174 (85.06%), Postives = 158/174 (90.80%), Query Frame = 0


Query: 1   MRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYN 60
           M T+SD VK KATPFDYGAGHV+PN+AMDPGLVYDTTIDDYLNFLCARGYN+ TL +F++
Sbjct: 598 MHTLSDTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHS 657

Query: 61  KPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVE 120
           KPFVCA  FTLTDLNYPSISIP+L+ GAPVTVNRRVKNVGTPGTYVARV ASSKI VTVE
Sbjct: 658 KPFVCATPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVE 717

Query: 121 PSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSD-GKHNVRSPIVVNLG 174
           PSTLQFNSVGEEKAFKV+FEYKG  Q K YVFGTLIWSD GKHNVRSPIVV LG
Sbjct: 718 PSTLQFNSVGEEKAFKVLFEYKGTGQGKSYVFGTLIWSDNGKHNVRSPIVVKLG 771

[Close]

BLAST of CSPI03G03830 vs. NCBI nr
Match: TYK19386.1 (subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa])

HSP 1 Score: 291.2 bits (744), Expect = 5.9e-75
Identity = 136/173 (78.61%), Postives = 153/173 (88.44%), Query Frame = 0


Query: 1    MRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYN 60
            M++I D  K KATPF YGAGHVHPN+A+DPGLVYDTTI+DY+NF+CA+GYNS TLK FYN
Sbjct: 1310 MKSILDYNKAKATPFQYGAGHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKRFYN 1369

Query: 61   KPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVE 120
            KP++C KSF LTDLNYPSIS+PKL  G PVT+NRR+KNVGTPGTYVARV  SSK+ VTV+
Sbjct: 1370 KPYLCPKSFPLTDLNYPSISVPKLTIGVPVTINRRLKNVGTPGTYVARVKVSSKVSVTVK 1429

Query: 121  PSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG 174
            PSTLQFNSVGEEKAFKVVFEYKG  Q KG+VFGTLIWSDGKH VRSP+VV LG
Sbjct: 1430 PSTLQFNSVGEEKAFKVVFEYKGKGQGKGHVFGTLIWSDGKHFVRSPMVVKLG 1482

[Close]

BLAST of CSPI03G03830 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 176.0 bits (445), Expect = 2.6e-44
Identity = 88/170 (51.76%), Postives = 110/170 (64.71%), Query Frame = 0


Query: 4   ISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYNKPF 63
           I +    KATPF +GAGHV PN A++PGLVYD  I DYLNFLC+ GYN+  +  F    F
Sbjct: 604 IQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNF 663

Query: 64  VCAK-SFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPS 123
            C+    +L +LNYPSI++P L   + VTV+R VKNVG P  Y  +VN    + V V+P+
Sbjct: 664 TCSSPKISLVNLNYPSITVPNLT-SSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPT 723

Query: 124 TLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNL 173
           +L F  VGE+K FKV+         KGYVFG L+WSD KH VRSPIVV L
Sbjct: 724 SLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

[Close]

BLAST of CSPI03G03830 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 154.5 bits (389), Expect = 8.1e-38
Identity = 81/163 (49.69%), Postives = 98/163 (60.12%), Query Frame = 0


Query: 11  KATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYNKP-FVCAKSF 70
           KA PF YG+GHV PN A  PGLVYD T  DYL+FLCA GYN+  ++ F   P + C +  
Sbjct: 617 KANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGA 676

Query: 71  TLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSV 130
            L D NYPSI++P L     +TV R++KNVG P TY AR      + V+VEP  L FN  
Sbjct: 677 NLLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKT 736

Query: 131 GEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNL 173
           GE K F++           GYVFG L W+D  H VRSPIVV L
Sbjct: 737 GEVKIFQMTLR-PLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

[Close]

BLAST of CSPI03G03830 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 132.1 bits (331), Expect = 4.3e-31
Identity = 65/163 (39.88%), Postives = 101/163 (61.96%), Query Frame = 0


Query: 12  ATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYNKPFVC--AKSF 71
           +TPFD+GAGHV P +A +PGL+YD T +DYL FLCA  Y S  +++   + + C  +KS+
Sbjct: 594 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 653

Query: 72  TLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNA-SSKILVTVEPSTLQFNS 131
           ++ DLNYPS ++     GA     R V +VG  GTY  +V + ++ + ++VEP+ L F  
Sbjct: 654 SVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKE 713

Query: 132 VGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVN 172
             E+K++ V F    ++      FG++ WSDGKH V SP+ ++
Sbjct: 714 ANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

[Close]

BLAST of CSPI03G03830 vs. TAIR 10
Match: AT4G15040.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.1e-29
Identity = 69/160 (43.12%), Postives = 96/160 (60.00%), Query Frame = 0


Query: 15  FDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYNKPFVCAK--SFTLT 74
           F YG+G V+P  A+DPGLVY+   +DYLN LC+  Y+S  +       F C++    T+ 
Sbjct: 526 FAYGSGFVNPTVAVDPGLVYEIAKEDYLNMLCSLDYSSQGISTIAGGTFTCSEQSKLTMR 585

Query: 75  DLNYPSISIP-KLQFGAPVTVNRRVKNVGTPG-TYVARVNASSKILVTVEPSTLQFNSVG 134
           +LNYPS+S        + +T +R V NVG  G TY A+++ + K+ + VEP+TL F + G
Sbjct: 586 NLNYPSMSAKVSASSSSDITFSRTVTNVGEKGSTYKAKLSGNPKLSIKVEPATLSFKAPG 645

Query: 135 EEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVV 171
           E+K+F V    K        V  +LIWSDG HNVRSPIVV
Sbjct: 646 EKKSFTVTVSGKSLAGISNIVSASLIWSDGSHNVRSPIVV 685

[Close]

BLAST of CSPI03G03830 vs. TAIR 10
Match: AT4G21326.1 (subtilase 3.12 )

HSP 1 Score: 122.9 bits (307), Expect = 2.6e-28
Identity = 69/163 (42.33%), Postives = 93/163 (57.06%), Query Frame = 0


Query: 12  ATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTLKNFYNKPFVCAKSF-T 71
           A PFDYGAG V+   A DPGLVYD  IDDY+++ CA GYN  ++     KP  C+    +
Sbjct: 590 ADPFDYGAGLVNAERAKDPGLVYDMNIDDYIHYFCATGYNDTSITIITGKPTKCSSPLPS 649

Query: 72  LTDLNYPSISIPKLQFGAPVTVNRRVKNVG-TPGTYVARVNASSKILVTVEPSTLQFNSV 131
           + DLNYP+I+IP L+    VTV R V NVG     Y A V     + + VEP TL F S 
Sbjct: 650 ILDLNYPAITIPDLE--EEVTVTRTVTNVGPVDSVYRAVVEPPRGVEIVVEPETLVFCSN 709

Query: 132 GEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNL 173
            ++  FKV      ++ + G+ FG+  W+DG  NV  P+ V +
Sbjct: 710 TKKLGFKVRVS-SSHKSNTGFFFGSFTWTDGTRNVTIPLSVRI 749

The following BLAST results are available for this feature:

BLAST of CSPI03G03830 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZSP5	3.6e-43	51.76	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=...	[more]
F4JXC5	1.1e-36	49.69	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S...	[more]
I1N462	1.9e-36	46.01	Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P...	[more]
O65351	6.0e-30	39.88	Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S...	[more]
O23357	1.5e-28	43.13	Subtilisin-like protease SBT4.2 OS=Arabidopsis thaliana OX=3702 GN=SBT4.2 PE=3 S...	[more]

BLAST of CSPI03G03830 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L601	5.6e-95	99.42	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045060 PE=3 SV=1	[more]
A0A1S3BLG8	2.1e-78	85.06	subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491134 PE=3 SV=...	[more]
A0A5D3D732	2.9e-75	78.61	Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A6J1KUL8	1.2e-68	77.06	uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820...	[more]
A0A6J1GDR2	3.8e-67	75.29	subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453063 PE...	[more]

BLAST of CSPI03G03830 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031738719.1	1.1e-94	99.42	uncharacterized protein LOC101212014 [Cucumis sativus]	[more]
KAE8650093.1	1.1e-94	99.42	hypothetical protein Csa_009608 [Cucumis sativus]	[more]
XP_038905686.1	1.4e-79	84.88	subtilisin-like protease SBT5.3 [Benincasa hispida]	[more]
XP_008449184.1	4.4e-78	85.06	PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo]	[more]
TYK19386.1	5.9e-75	78.61	subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa]	[more]

BLAST of CSPI03G03830 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G04160.1	2.6e-44	51.76	Subtilisin-like serine endopeptidase family protein	[more]
AT5G59810.1	8.1e-38	49.69	Subtilase family protein	[more]
AT5G67360.1	4.3e-31	39.88	Subtilase family protein	[more]
AT4G15040.1	1.1e-29	43.13	Subtilisin-like serine endopeptidase family protein	[more]
AT4G21326.1	2.6e-28	42.33	subtilase 3.12	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 73..170 e-value: 1.1E-29 score: 102.5
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 7..41 e-value: 6.5E-10 score: 40.4
None	No IPR available	GENE3D	2.60.40.2310		coord: 45..172 e-value: 9.6E-37 score: 127.6
None	No IPR available	PANTHER	PTHR43806	PEPTIDASE S8	coord: 3..171
None	No IPR available	PANTHER	PTHR43806:SF34	SUBTILISIN-LIKE PROTEASE SBT5.4	coord: 3..171

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G03830.1	CSPI03G03830.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009610	response to symbiotic fungus
molecular_function	GO:0004252	serine-type endopeptidase activity