CSPI03G03210 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G03210
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	methylsterol monooxygenase 1-2-like
Location	Chr3: 2593478 .. 2598135 (-)
RNA-Seq Expression	CSPI03G03210
Synteny	CSPI03G03210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGAGAAGATTTGGAAGGGAGTGTTGGAAATGGTGGATTGCCATTCTCACATATAAATATACAAAACACAAATAACCAAACAAATATTGATTGAATCCATAGGATTTCACGCTCTTCCTCTTGTCTTCCTCCTCTTCCTCCTCTTCTTCCTTCTTCCATTTTCCCTTTTCAAACTTCCCGCATTAATTTAACTGTTTCTCACCCTTCTCCCTCTTCCATTTCACTCCTTTTCGCCGTTTCTCAATCCATTCACCGCCGGCGAACTTCTCTCTCTCTTTTTTCCATCTGGGTCTCTCTCCGCCGGCGGATTCCTTCACCACATGTTGCCCTTTCACTCCCTCTCCGCCGCTCAGGCCTCCCTTGGCCGGAATCTCACTTTCTTTGAGACCCTTTGGTTTAATTACTCCAATGACAAATCCGATTACTTCCTCTTTTGCCACAACATTCTCTTCCTTTTCTTGATTTTCTCCTTTATTCCCCTTCCCCTTATCTTCCTTGAGCTTTTGCATGCTACTGGGATTCATAAGTACAAAATTCAGCCCAAGGTTCGATTGCCATTCAACGAGATCTTCCGGTGCTACAAGGATGTTATGCGTATGTTCTTCCTTGTTGTTGGCCCTCTTCAGCTCGTTTCGTTCCCTTCCATTAAGGTGAATCTTGGGTTGTTTTTTTTTATTTGCTTCCTTTTTCTTGATCTGGGATTTTGATTTTGATTGTTTGTTTTCTGTTTCTTTTGAGTTATTAGATGATTGGGATCAGAACGGGTCTACCATTGCCTTCTGGGTTTGAAATTGTTTCTCAATTGGTGGTGTATTTCATGGTGGAGGATTATACCAATTATTGGATTCATAGATTCCTTCATTGCAAATGGGGGTATGAGAAAATCCATTGTGTTCACCATGAGTACACTGCTCCTATTGGATTTGCAGCTCCTTATGCTCATTGGGCTGAGGTTTTGATCCTTGGAATTCCCTCATTTCTTGGTCCAGCTATGGTTCCTGGTCATATGATCACCTTCTGGTTATGGATTGCTTTGAGGCAGATTGAGGCCATTGATACACATAGTGGGTATGAATGCTAAAGCTATTTCTCTTCCTCTCTGTCTCTCACTCTCTATCTAGCTTTCTTGCTTTCTATTTTAATAAACTTCCTGCCTTTTTTTTTTTTTTTTTTTCACTTCTGCACTGTGCTTTTATTCGACTGCGACAACATGATGCCAACTTTGTATTTTCTGTATTCTTTTATCTCTTCACCAATGCCTTTGTGTTAAAGTACTTTTAATCTCAAGGATCTTGAATGGTGGGCTTGCCTCTTTTTTGACTAGGACCGTGATCTGCTCTATGGTTTTCATATTTTTTAAATCTTGGTTGCCTTTCCTTTATTATATATATTTTCTATAAGTGTAGATATATGTTTAGTCGCAATATAGAGTTCCTATCATTACTGGATGAGCTCATTGCATGCTGAATCATTGCTCCTACTAACTTGATATAATGAAAGTTTGTTGAGAAATATTGTAAAGGTTAAATTTTACCCATCTTTTCTAGCATCAACTTTCTCTGCTTCCACAATCGCCATACAGTTGTGGGAAAGCCTAAGATTCTTTTGAACTTATTGTAAGAGGATTTATAAATCTTCTCTGAGAAGCGGAGCCTATGAAGCTTGATTCTCTTATAAACTAAACTAATTAGGGCCCCACTACCCTTTTATGCTTTAAGCTTATATACATCATTTCAAGATCTATGATTTAACTGGCCATGAAAGAGGCAAGTCTTGAATTATAGAACAATGTTATTGTTAATTGTTGTTATTAGTTTGTTCTAGCAATTTTGTATTTATTACATTTGTGCTAACGCATGACTCAGGTAATGTGGACTAGTAGTACTGATAATTTGTAAAAAGTAAAAAAATGAATTTATCCCAGCTGATCAAAGCTCGGTAGAGGATAACAATATTTGACTTTACTTTCTCCATTTAGTGGGACCATAGATATTAGAGTATAGGTTTTAGAGAAGAATGAAATTTGCGTATGAGGGAAGGAAAAACAACAAAGTGATTGCGTCAATGTATTTTCATCAATGGTAGGTAAGTACAATGAAATATTAGGTAGATTGTGAAGTGATGCTGCAACGATATTGATGGGCGAAGATCATGGACTAGATCATAACTGAACCTTATCGGTTCAAACATTCTTAACTTCAGGACCTTTTAGCTCACAACCATCCCCAAATTTCCCGACCTTATTGCATTGTAGTTGTTATGAGGCTTCATGTTTACTCCCTTGTGGAAGTCTGCCTTTCCAAACTCAATGTCAAATTCTGTAGACTTAGGGTTGTTGGGAAAAGAAAGTTAAAAAAAGGAGTTGATGTGTAGAAAGACATCTTTAGAGAATTGCTTCTAGCATGATGGAATAGACATGTAGGGGCGCTCGGGGGTTAAACTCGTGTCATTTGAGGTTAAGAAGTGGAATGTATTCTAGCATATGGTTTGCTTATCTTGAGCTATGAGTTTGTTAGTTCCATGGGCTACATAAACCCACTAGTTATGCAGACCCAAGTTTCCATAACCTATATTGGTACAGTCTAAACACTAATTAATTATATGAACTCAGCTTTTCAATAAACCAACCTTATTAAACTGTATTGTTCCTATTCCTCAGAAGAATCAAAAGCAAGCTCTGTTATTCATGTTGACTGCATTTGTTTTAAAACTATTATATTTAGAGGAATGAAGAGAGAAAGAAAAGTTAGTTGACATCTAAAGGGGCCAGCTTACTCAAATAAGAATCCATTGATTTGACCTCCAAATTGTTTAGTTGGGCCAAAAACAAAAAATAATTGATGGCAGGCTTATGGCTGGGAATAAATGCGGTGTCAGATAATCATTCCTTAGGTCATGTAGGACATAGCTTAACTCTTGGTGGGTTTCCTGTGGCTGAACATCCCCTTAGCCTTTGGTTGAACTTGAGTAACTTTATGTACTATTATTTTTAATTGCTGAAGTTTGATAGTTGTCTTTTTCATACGAAAACTGAGATTCTCATGAACATTGATGGCAAGATGTTGTACAGTTTTGAAAAGGAAATTCAAAATTAAAGTCTTCTACTCACAACAGTTATCTGAAGTTGAAGGATTTTCTTATCAATGTGTTTCACAAGCATTAGCGTGAATGAACTGTCGACCTTTTCATTCTTAATCGATTTATTAATTTTCGTTTAAATTACCAGTATAATTCTTATATTTTGAGCTTGATTTCAGCTTGGTCATGGTAGTTATAGAAGTTCAATTATCATCTTTCGTTGCTAGGTGTTAATAGTTTTCTTATGTGTCATGTTTTCAAAGGACGTGATAGAGAGCAGTGACAAGATGATGTTTAGTATAAATCATGTGTGAAGCCTTACTTCATATCTTCAATAATCCTAATCTAACCTGCAAAAACATACGAGCAGAGACGTTTGGGGAGGTATAACCAAACAACAGGGACTACAATGAACTTTTGAGATAACATTGAAACCAAGCACAGACCATATGGACCAAAATTGTAATATAACCTTATTTTTGTTACAGATAAACTATTGATGAAAACTACATCATTTCCTTGTTCTTTTTTGTGGTCTTTCTTTCTTTACTTCTGCTTTTCTCTTATATTGTGCTTTTATGATGATTGTTGAGATCAACCATGGTTTTATTCATATATTTAATATTTATATTCAGGTATGATTTCCCTTGGAGTCTAACAAAATTTATACCATTTTATGGTGGTGCGGACCATCATGATTACCATCACTATGTTGGAGGGCAAAGCCATAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGTAGTTATAGTCTTGTTATAATTTTACCTCCCACTTGTTCTTCCTATGAAATCATGCTCACGAAAGTTACTCATTTGTTTACTTTCTCAGGGGTATCGCTATCAAAAGAAGATTCTTCAGAAGGTAAGCGCTTTGTTTTCCCCGCCCCCCACCTTCACCCTTAAAATTGATTGCTGCATATGGTGTCATGAATTAGCAAGTTTTTTTCAAATAGACAAAATTGAGCTTGGTTCAATGGCATGCCCGGATAAGCTTGAACAAGCCTTAGGATGGGCAGTTTAACTATATTTACTATGACTTTCTCCTGTTCTGTTTGGTTGGATCAAATCTATTCCATCTATTCCATTTGTCATTGTGATTTGTTTTGGACTGATATCGTGTGTTCTAATTGTTTGGTTTTGGTGTAATTAGTTGAAGGAAGAAGTGAAGAACAGTGAAGAGTCATACTACAACACAGCTCAAAATGTTAAATCGGACTAGGCGATTCATATTTTGAAGGATGGATTTTTTTTGGGTCGGTAGCAACGTTACGTTACATACATGATCTCTGATTTGAAGTTTACTTGGCCAGGGACGATATCATCAACAAGGATGTAGTTTGGGAGTGGGAAGGGATGGAAATTAACATGGGGTCTATAAAGTTTGTTTTTTTTTTGTTTTTTTGGTTAGCTTAATCTTCAGGGCTCAGAATTCAGATTCTCCAATGGTGTGGTTTAACCTTATTGTGCAGACAAAACTCCTAATATATATAAATATGTATTCTGCTTTTGAATTTTTCTTGATTTTATTTGCCAACC

mRNA sequence

GTGAGAAGATTTGGAAGGGAGTGTTGGAAATGGTGGATTGCCATTCTCACATATAAATATACAAAACACAAATAACCAAACAAATATTGATTGAATCCATAGGATTTCACGCTCTTCCTCTTGTCTTCCTCCTCTTCCTCCTCTTCTTCCTTCTTCCATTTTCCCTTTTCAAACTTCCCGCATTAATTTAACTGTTTCTCACCCTTCTCCCTCTTCCATTTCACTCCTTTTCGCCGTTTCTCAATCCATTCACCGCCGGCGAACTTCTCTCTCTCTTTTTTCCATCTGGGTCTCTCTCCGCCGGCGGATTCCTTCACCACATGTTGCCCTTTCACTCCCTCTCCGCCGCTCAGGCCTCCCTTGGCCGGAATCTCACTTTCTTTGAGACCCTTTGGTTTAATTACTCCAATGACAAATCCGATTACTTCCTCTTTTGCCACAACATTCTCTTCCTTTTCTTGATTTTCTCCTTTATTCCCCTTCCCCTTATCTTCCTTGAGCTTTTGCATGCTACTGGGATTCATAAGTACAAAATTCAGCCCAAGGTTCGATTGCCATTCAACGAGATCTTCCGGTGCTACAAGGATGTTATGCGTATGTTCTTCCTTGTTGTTGGCCCTCTTCAGCTCGTTTCGTTCCCTTCCATTAAGATGATTGGGATCAGAACGGGTCTACCATTGCCTTCTGGGTTTGAAATTGTTTCTCAATTGGTGGTGTATTTCATGGTGGAGGATTATACCAATTATTGGATTCATAGATTCCTTCATTGCAAATGGGGGTATGAGAAAATCCATTGTGTTCACCATGAGTACACTGCTCCTATTGGATTTGCAGCTCCTTATGCTCATTGGGCTGAGGTTTTGATCCTTGGAATTCCCTCATTTCTTGGTCCAGCTATGGTTCCTGGTCATATGATCACCTTCTGGTTATGGATTGCTTTGAGGCAGATTGAGGCCATTGATACACATAGTGGGTATGATTTCCCTTGGAGTCTAACAAAATTTATACCATTTTATGGTGGTGCGGACCATCATGATTACCATCACTATGTTGGAGGGCAAAGCCATAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGGGTATCGCTATCAAAAGAAGATTCTTCAGAAGTTGAAGGAAGAAGTGAAGAACAGTGAAGAGTCATACTACAACACAGCTCAAAATGTTAAATCGGACTAGGCGATTCATATTTTGAAGGATGGATTTTTTTTGGGTCGGTAGCAACGTTACGTTACATACATGATCTCTGATTTGAAGTTTACTTGGCCAGGGACGATATCATCAACAAGGATGTAGTTTGGGAGTGGGAAGGGATGGAAATTAACATGGGGTCTATAAAGTTTGTTTTTTTTTTGTTTTTTTGGTTAGCTTAATCTTCAGGGCTCAGAATTCAGATTCTCCAATGGTGTGGTTTAACCTTATTGTGCAGACAAAACTCCTAATATATATAAATATGTATTCTGCTTTTGAATTTTTCTTGATTTTATTTGCCAACC

Coding sequence (CDS)

ATGTTGCCCTTTCACTCCCTCTCCGCCGCTCAGGCCTCCCTTGGCCGGAATCTCACTTTCTTTGAGACCCTTTGGTTTAATTACTCCAATGACAAATCCGATTACTTCCTCTTTTGCCACAACATTCTCTTCCTTTTCTTGATTTTCTCCTTTATTCCCCTTCCCCTTATCTTCCTTGAGCTTTTGCATGCTACTGGGATTCATAAGTACAAAATTCAGCCCAAGGTTCGATTGCCATTCAACGAGATCTTCCGGTGCTACAAGGATGTTATGCGTATGTTCTTCCTTGTTGTTGGCCCTCTTCAGCTCGTTTCGTTCCCTTCCATTAAGATGATTGGGATCAGAACGGGTCTACCATTGCCTTCTGGGTTTGAAATTGTTTCTCAATTGGTGGTGTATTTCATGGTGGAGGATTATACCAATTATTGGATTCATAGATTCCTTCATTGCAAATGGGGGTATGAGAAAATCCATTGTGTTCACCATGAGTACACTGCTCCTATTGGATTTGCAGCTCCTTATGCTCATTGGGCTGAGGTTTTGATCCTTGGAATTCCCTCATTTCTTGGTCCAGCTATGGTTCCTGGTCATATGATCACCTTCTGGTTATGGATTGCTTTGAGGCAGATTGAGGCCATTGATACACATAGTGGGTATGATTTCCCTTGGAGTCTAACAAAATTTATACCATTTTATGGTGGTGCGGACCATCATGATTACCATCACTATGTTGGAGGGCAAAGCCATAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGGGTATCGCTATCAAAAGAAGATTCTTCAGAAGTTGAAGGAAGAAGTGAAGAACAGTGAAGAGTCATACTACAACACAGCTCAAAATGTTAAATCGGACTAG

Protein sequence

MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLELLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPLPSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESYYNTAQNVKSD*

Homology

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BLAST of CSPI03G03210 vs. ExPASy Swiss-Prot
Match: Q1EC69 (Methylsterol monooxygenase 1-2 OS=Arabidopsis thaliana OX=3702 GN=SMO1-2 PE=2 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 8.6e-133
Identity = 217/290 (74.83%), Postives = 258/290 (88.97%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A  +L RNLT+ ETLWF+YS  KSDY+L+CHNILFLFLIFS +PLPL+F+E
Sbjct: 1   MIPYATIEEASIALSRNLTWLETLWFDYSATKSDYYLYCHNILFLFLIFSLVPLPLVFIE 60

Query: 61  LLHATG--IHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGL 120
              +T    ++YKIQPKV+  F+ +F+CYKDVM+MF LVVGPLQLVS+PSI+MI IR+GL
Sbjct: 61  SSQSTSDLFNRYKIQPKVKNSFSSMFKCYKDVMKMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWA 180
           PLPS  EIV+QLVVYF+VEDYTNYW+HRF HCKWGYEK H +HHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPSCMEIVAQLVVYFLVEDYTNYWVHRFFHCKWGYEKFHHIHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHH 240
           EVL+LGIP+FLGPA+ PGHMITFWLWIALRQIEAI+THSGYDFPWSLTK+IPFYGGA++H
Sbjct: 181 EVLLLGIPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEE 289
           DYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+LQ++KE+ K S +
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQMKEKSKKSNK 290

[Close]

BLAST of CSPI03G03210 vs. ExPASy Swiss-Prot
Match: Q8L7W5 (Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana OX=3702 GN=SMO1-1 PE=1 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 2.9e-128
Identity = 211/290 (72.76%), Postives = 257/290 (88.62%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A  +LGRNLT  ETLWF+YS  KSDY+L+CHNILFLFL+FS +PLPL+F+E
Sbjct: 1   MIPYATVEEASIALGRNLTRLETLWFDYSATKSDYYLYCHNILFLFLVFSLVPLPLVFVE 60

Query: 61  LLH-ATGI-HKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGL 120
           L   A+G+ ++YKIQPKV    +++F+CYKDVM MF LVVGPLQLVS+PSI+MI IR+GL
Sbjct: 61  LARSASGLFNRYKIQPKVNYSLSDMFKCYKDVMTMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWA 180
           PLP+  E++SQLVVYF++EDYTNYW+HRF H KWGY+KIH VHHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPTITEMLSQLVVYFLIEDYTNYWVHRFFHSKWGYDKIHRVHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHH 240
           EVL+LGIP+F+GPA+ PGHMITFWLWIALRQ+EAI+THSGYDFPWS TK+IPFYGGA++H
Sbjct: 181 EVLLLGIPTFMGPAIAPGHMITFWLWIALRQMEAIETHSGYDFPWSPTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEE 289
           DYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L+++KE  K S +
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQIKESSKKSNK 290

[Close]

BLAST of CSPI03G03210 vs. ExPASy Swiss-Prot
Match: F4JLZ6 (Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana OX=3702 GN=SMO1-3 PE=2 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 6.0e-118
Identity = 193/286 (67.48%), Postives = 246/286 (86.01%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A  +LGRNLT+FET+WF+YS  KS++ ++CH IL LFL+FS  P PL+ +E
Sbjct: 1   MIPYPTVEDASVALGRNLTWFETVWFDYSATKSNFHVYCHTILVLFLVFSLAPFPLVIVE 60

Query: 61  LLHATG-IHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLP 120
               TG   ++KIQ KV+   +++F+CYK+VM++F LVVG LQ+VS+PSI+M+GIR+GLP
Sbjct: 61  W---TGWFDQFKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLP 120

Query: 121 LPSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAE 180
           LPS  EIV+QLVVYF++EDYTNYWIHR++HCKWGYEKIH +HHEYT+PIG+A+PYAHWAE
Sbjct: 121 LPSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAE 180

Query: 181 VLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHD 240
           +LILGIP+FLGPA+ PGH++TFWLWI+LRQ EAI+THSGYDFPWS+TK IPFYGG ++HD
Sbjct: 181 ILILGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHD 240

Query: 241 YHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL-QKLKEEVK 285
           YHHYVGGQS SNFASVFTYCDYIYGTDKGYR  KK+L  ++KEE +
Sbjct: 241 YHHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLLHHQIKEEAE 283

[Close]

BLAST of CSPI03G03210 vs. ExPASy Swiss-Prot
Match: Q9AV39 (Very-long-chain aldehyde decarbonylase GL1-10 OS=Oryza sativa subsp. japonica OX=39947 GN=GL1-10 PE=2 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 1.3e-117
Identity = 196/280 (70.00%), Postives = 235/280 (83.93%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLP+ + + A+A+LGR +T  E+LWF YS    DY LF HNILFLF++F+  PLP+  LE
Sbjct: 1   MLPYATAAEAEAALGRAMTAAESLWFRYSAGIPDYVLFWHNILFLFVVFTLAPLPVALLE 60

Query: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120
           L     +  +K+QPKVRL   E FRCY+DVMR+FFLV+GPLQLVS+P++KM+GI TGLPL
Sbjct: 61  LRAPAAVGPFKLQPKVRLSREEFFRCYRDVMRLFFLVIGPLQLVSYPTVKMVGIHTGLPL 120

Query: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180
           PS  E+ +QL+VYF+VEDY NYWIHR LH +WGYEKIH VHHE+TAPIGFAAPYAHWAEV
Sbjct: 121 PSLGEMAAQLLVYFLVEDYLNYWIHRLLHGEWGYEKIHRVHHEFTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSF+GPA+ PGHMITFWLWI LRQ+EAI+THSG+DFP++LTK+IPFYGGA++HDY
Sbjct: 181 LILGIPSFVGPALAPGHMITFWLWIVLRQMEAIETHSGFDFPFNLTKYIPFYGGAEYHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLK 281
           HHYVG QS SNFASVFTYCDY+YGTDKGYRY K    K+K
Sbjct: 241 HHYVGRQSQSNFASVFTYCDYLYGTDKGYRYHKAYQAKMK 280

[Close]

BLAST of CSPI03G03210 vs. ExPASy Swiss-Prot
Match: Q8VWZ8 (Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana OX=3702 GN=SMO2-2 PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.3e-56
Identity = 113/258 (43.80%), Postives = 154/258 (59.69%), Query Frame = 0


Query: 19  TFFETLWFNYSNDKSDYFLFCHNILFLF-LIFSFIPLPLIFLELLHATGIHKYKIQPKVR 78
           +F E+ W       SD+ L C     L   +F    LP IFLE      + KYKIQ K  
Sbjct: 3   SFVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLE--RQGFLSKYKIQTKNN 62

Query: 79  LPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPLPSGFEIVSQLVVYFMVE 138
            P  +  +C   ++   F V  PL L S+P  + +G+R+  PLPS  E+ +Q++ YF++E
Sbjct: 63  TPAAQ-GKCITRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIE 122

Query: 139 DYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEVLILGIPSFLGPAMVPGH 198
           D+  YW HR LH KW Y+ +H VHHEY  P G  + YAH AE+L LG  + +GPA+   H
Sbjct: 123 DFVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPH 182

Query: 199 MITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFT 258
           +IT WLW+ LR +E ++ H GY FPWSL+ F+P YGGAD HDYHH +      N++S F 
Sbjct: 183 LITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFV 242

Query: 259 YCDYIYGTDKGYRYQKKI 276
           Y D+I+GTDKGYR  K +
Sbjct: 243 YMDWIFGTDKGYRRLKTL 257

[Close]

BLAST of CSPI03G03210 vs. ExPASy TrEMBL
Match: A0A0A0L5P8 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G036480 PE=3 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 5.0e-176
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120
           LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL
Sbjct: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120

Query: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180
           PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESYYNTAQNVKSD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESYYNTAQNVKSD
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESYYNTAQNVKSD 300

[Close]

BLAST of CSPI03G03210 vs. ExPASy TrEMBL
Match: A0A1S3BKU6 (methylsterol monooxygenase 1-2-like OS=Cucumis melo OX=3656 GN=LOC103490939 PE=3 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.2e-172
Identity = 297/301 (98.67%), Postives = 299/301 (99.34%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHSLSAAQASLGRNLTFFETLWFNYS+DKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120
           LLHA+GI KYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL
Sbjct: 61  LLHASGIRKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120

Query: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180
           PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEE-SYYNTAQNVKS 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEE SYYNTAQNVKS
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESSYYNTAQNVKS 300

[Close]

BLAST of CSPI03G03210 vs. ExPASy TrEMBL
Match: A0A6J1E9U9 (methylsterol monooxygenase 1-2 OS=Cucurbita moschata OX=3662 GN=LOC111431186 PE=3 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 8.0e-166
Identity = 282/300 (94.00%), Postives = 295/300 (98.33%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHSLSAAQ +LGRNLTFFETLWFNYS+DKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120
           +LHA+GIHKYKIQPKVRLPF+EIFRCYKDVMRMFFLVVGPLQLVSFPSI+MIGIRTGLPL
Sbjct: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180
           PSG+EIVSQLVVYFMVEDYTNYWIHRFLHCKWGYE IH VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGG+DHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGSDHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESYYNTAQNVKSD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL+KLKEEVK+SE SYY+TA++VK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

[Close]

BLAST of CSPI03G03210 vs. ExPASy TrEMBL
Match: A0A6J1HQ57 (methylsterol monooxygenase 1-2 OS=Cucurbita maxima OX=3661 GN=LOC111465081 PE=3 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 1.8e-165
Identity = 281/300 (93.67%), Postives = 295/300 (98.33%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHS+SAAQ +LGRNLTFFETLWFNYS+DKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSISAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120
           +LHA+GIHK+KIQPKVRLPF+EIFRCYKDVMRMFFLVVGPLQLVSFPSI+MIGIRTGLPL
Sbjct: 61  ILHASGIHKFKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180
           PSG+EIVSQLVVYFMVEDYTNYWIHRFLHCKWGYE IH VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESYYNTAQNVKSD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL+KLKEEVK+SE SYY+TA++VK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

[Close]

BLAST of CSPI03G03210 vs. ExPASy TrEMBL
Match: A0A6J1FUF2 (methylsterol monooxygenase 1-1-like OS=Cucurbita moschata OX=3662 GN=LOC111448229 PE=3 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 2.2e-160
Identity = 274/300 (91.33%), Postives = 287/300 (95.67%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHSLSAAQA+LGRNLT FETLWFNYS+DKS YFL+CHNILFLFLIFS IPLPLIFLE
Sbjct: 1   MLPFHSLSAAQAALGRNLTSFETLWFNYSHDKSSYFLYCHNILFLFLIFSLIPLPLIFLE 60

Query: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120
           L H +GIHKYKIQPKVRLP  EIFRCYKDVMRMFFLVVGPLQLVS+PSI MIGIRTGLPL
Sbjct: 61  LFHVSGIHKYKIQPKVRLPSAEIFRCYKDVMRMFFLVVGPLQLVSYPSINMIGIRTGLPL 120

Query: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180
           PSG+EIVSQLVVYFMVEDYTNYW+HRFLHC+WGYEKIH VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWVHRFLHCEWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAI+THSGYDFPWS+TK IPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSVTKLIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESYYNTAQNVKSD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL+KLKEE K SE SYY+TAQ+VKSD
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKISEASYYSTAQDVKSD 300

[Close]

BLAST of CSPI03G03210 vs. NCBI nr
Match: XP_011650426.1 (methylsterol monooxygenase 1-2 [Cucumis sativus] >KGN55922.1 hypothetical protein Csa_009711 [Cucumis sativus])

HSP 1 Score: 626.7 bits (1615), Expect = 1.0e-175
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120
           LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL
Sbjct: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120

Query: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180
           PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESYYNTAQNVKSD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESYYNTAQNVKSD
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESYYNTAQNVKSD 300

[Close]

BLAST of CSPI03G03210 vs. NCBI nr
Match: XP_008448914.1 (PREDICTED: methylsterol monooxygenase 1-2-like [Cucumis melo])

HSP 1 Score: 615.5 bits (1586), Expect = 2.4e-172
Identity = 297/301 (98.67%), Postives = 299/301 (99.34%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHSLSAAQASLGRNLTFFETLWFNYS+DKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120
           LLHA+GI KYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL
Sbjct: 61  LLHASGIRKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120

Query: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180
           PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEE-SYYNTAQNVKS 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEE SYYNTAQNVKS
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESSYYNTAQNVKS 300

[Close]

BLAST of CSPI03G03210 vs. NCBI nr
Match: XP_038876881.1 (methylsterol monooxygenase 1-2-like [Benincasa hispida])

HSP 1 Score: 600.5 bits (1547), Expect = 7.9e-168
Identity = 285/300 (95.00%), Postives = 297/300 (99.00%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHSLSAAQA+LGRNLTFFETLWFNYS+DKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120
           LLHA+GIHKYKIQPKVRLPF+EIFRCYKDVMRMFFLVVGPLQLVSFPSI+MIGIRTGLPL
Sbjct: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180
           PSG+EI+SQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIISQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESYYNTAQNVKSD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL++LKEEVKN E  Y++TAQ+VKSD
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKQLKEEVKNIEGPYHSTAQDVKSD 300

[Close]

BLAST of CSPI03G03210 vs. NCBI nr
Match: XP_023552163.1 (methylsterol monooxygenase 1-2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 594.0 bits (1530), Expect = 7.4e-166
Identity = 283/300 (94.33%), Postives = 295/300 (98.33%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHSLSAAQ +LGRNLTFFETLWFNYS+DKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120
           +LHA+GIHKYKIQPKVRLPF+EIFRCYKDVMRMFFLVVGPLQLVSFPSI+MIGIRTGLPL
Sbjct: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180
           PSG+EIVSQLVVYFMVEDYTNYWIHRFLHCKWGYE IH VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESYYNTAQNVKSD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL+KLKEEVK+SE SYY+TA++VK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

[Close]

BLAST of CSPI03G03210 vs. NCBI nr
Match: XP_022923513.1 (methylsterol monooxygenase 1-2 [Cucurbita moschata] >KAG6577591.1 Methylsterol monooxygenase 1-2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015639.1 Methylsterol monooxygenase 1-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 592.8 bits (1527), Expect = 1.6e-165
Identity = 282/300 (94.00%), Postives = 295/300 (98.33%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHSLSAAQ +LGRNLTFFETLWFNYS+DKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120
           +LHA+GIHKYKIQPKVRLPF+EIFRCYKDVMRMFFLVVGPLQLVSFPSI+MIGIRTGLPL
Sbjct: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180
           PSG+EIVSQLVVYFMVEDYTNYWIHRFLHCKWGYE IH VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGG+DHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGSDHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESYYNTAQNVKSD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL+KLKEEVK+SE SYY+TA++VK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

[Close]

BLAST of CSPI03G03210 vs. TAIR 10
Match: AT4G22756.1 (sterol C4-methyl oxidase 1-2 )

HSP 1 Score: 474.6 bits (1220), Expect = 6.1e-134
Identity = 217/290 (74.83%), Postives = 258/290 (88.97%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A  +L RNLT+ ETLWF+YS  KSDY+L+CHNILFLFLIFS +PLPL+F+E
Sbjct: 1   MIPYATIEEASIALSRNLTWLETLWFDYSATKSDYYLYCHNILFLFLIFSLVPLPLVFIE 60

Query: 61  LLHATG--IHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGL 120
              +T    ++YKIQPKV+  F+ +F+CYKDVM+MF LVVGPLQLVS+PSI+MI IR+GL
Sbjct: 61  SSQSTSDLFNRYKIQPKVKNSFSSMFKCYKDVMKMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWA 180
           PLPS  EIV+QLVVYF+VEDYTNYW+HRF HCKWGYEK H +HHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPSCMEIVAQLVVYFLVEDYTNYWVHRFFHCKWGYEKFHHIHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHH 240
           EVL+LGIP+FLGPA+ PGHMITFWLWIALRQIEAI+THSGYDFPWSLTK+IPFYGGA++H
Sbjct: 181 EVLLLGIPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEE 289
           DYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+LQ++KE+ K S +
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQMKEKSKKSNK 290

[Close]

BLAST of CSPI03G03210 vs. TAIR 10
Match: AT4G12110.1 (sterol-4alpha-methyl oxidase 1-1 )

HSP 1 Score: 459.5 bits (1181), Expect = 2.0e-129
Identity = 211/290 (72.76%), Postives = 257/290 (88.62%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A  +LGRNLT  ETLWF+YS  KSDY+L+CHNILFLFL+FS +PLPL+F+E
Sbjct: 1   MIPYATVEEASIALGRNLTRLETLWFDYSATKSDYYLYCHNILFLFLVFSLVPLPLVFVE 60

Query: 61  LLH-ATGI-HKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGL 120
           L   A+G+ ++YKIQPKV    +++F+CYKDVM MF LVVGPLQLVS+PSI+MI IR+GL
Sbjct: 61  LARSASGLFNRYKIQPKVNYSLSDMFKCYKDVMTMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWA 180
           PLP+  E++SQLVVYF++EDYTNYW+HRF H KWGY+KIH VHHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPTITEMLSQLVVYFLIEDYTNYWVHRFFHSKWGYDKIHRVHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHH 240
           EVL+LGIP+F+GPA+ PGHMITFWLWIALRQ+EAI+THSGYDFPWS TK+IPFYGGA++H
Sbjct: 181 EVLLLGIPTFMGPAIAPGHMITFWLWIALRQMEAIETHSGYDFPWSPTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEE 289
           DYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L+++KE  K S +
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQIKESSKKSNK 290

[Close]

BLAST of CSPI03G03210 vs. TAIR 10
Match: AT4G22753.2 (sterol 4-alpha methyl oxidase 1-3 )

HSP 1 Score: 426.0 bits (1094), Expect = 2.5e-119
Identity = 197/296 (66.55%), Postives = 251/296 (84.80%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A  +LGRNLT+FET+WF+YS  KS++ ++CH IL LFL+FS  P PL+ +E
Sbjct: 1   MIPYPTVEDASVALGRNLTWFETVWFDYSATKSNFHVYCHTILVLFLVFSLAPFPLVIVE 60

Query: 61  LLHATG-IHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLP 120
               TG   ++KIQ KV+   +++F+CYK+VM++F LVVG LQ+VS+PSI+M+GIR+GLP
Sbjct: 61  W---TGWFDQFKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLP 120

Query: 121 LPSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAE 180
           LPS  EIV+QLVVYF++EDYTNYWIHR++HCKWGYEKIH +HHEYT+PIG+A+PYAHWAE
Sbjct: 121 LPSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAE 180

Query: 181 VLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHD 240
           +LILGIP+FLGPA+ PGH++TFWLWI+LRQ EAI+THSGYDFPWS+TK IPFYGG ++HD
Sbjct: 181 ILILGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHD 240

Query: 241 YHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL-QKLKEEVKNSEESYYNTA 295
           YHHYVGGQS SNFASVFTYCDYIYGTDKGYR  KK+L  ++KEE   +EE  + TA
Sbjct: 241 YHHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLLHHQIKEE---AEEKRFCTA 290

[Close]

BLAST of CSPI03G03210 vs. TAIR 10
Match: AT4G22753.1 (sterol 4-alpha methyl oxidase 1-3 )

HSP 1 Score: 425.2 bits (1092), Expect = 4.2e-119
Identity = 193/286 (67.48%), Postives = 246/286 (86.01%), Query Frame = 0


Query: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A  +LGRNLT+FET+WF+YS  KS++ ++CH IL LFL+FS  P PL+ +E
Sbjct: 1   MIPYPTVEDASVALGRNLTWFETVWFDYSATKSNFHVYCHTILVLFLVFSLAPFPLVIVE 60

Query: 61  LLHATG-IHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLP 120
               TG   ++KIQ KV+   +++F+CYK+VM++F LVVG LQ+VS+PSI+M+GIR+GLP
Sbjct: 61  W---TGWFDQFKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLP 120

Query: 121 LPSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAE 180
           LPS  EIV+QLVVYF++EDYTNYWIHR++HCKWGYEKIH +HHEYT+PIG+A+PYAHWAE
Sbjct: 121 LPSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAE 180

Query: 181 VLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHD 240
           +LILGIP+FLGPA+ PGH++TFWLWI+LRQ EAI+THSGYDFPWS+TK IPFYGG ++HD
Sbjct: 181 ILILGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHD 240

Query: 241 YHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL-QKLKEEVK 285
           YHHYVGGQS SNFASVFTYCDYIYGTDKGYR  KK+L  ++KEE +
Sbjct: 241 YHHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLLHHQIKEEAE 283

[Close]

BLAST of CSPI03G03210 vs. TAIR 10
Match: AT1G07420.1 (sterol 4-alpha-methyl-oxidase 2-1 )

HSP 1 Score: 221.5 bits (563), Expect = 9.3e-58
Identity = 113/258 (43.80%), Postives = 154/258 (59.69%), Query Frame = 0


Query: 19  TFFETLWFNYSNDKSDYFLFCHNILFLF-LIFSFIPLPLIFLELLHATGIHKYKIQPKVR 78
           +F E+ W       SD+ L C     L   +F    LP IFLE      + KYKIQ K  
Sbjct: 3   SFVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLE--RQGFLSKYKIQTKNN 62

Query: 79  LPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPLPSGFEIVSQLVVYFMVE 138
            P  +  +C   ++   F V  PL L S+P  + +G+R+  PLPS  E+ +Q++ YF++E
Sbjct: 63  TPAAQ-GKCITRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIE 122

Query: 139 DYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEVLILGIPSFLGPAMVPGH 198
           D+  YW HR LH KW Y+ +H VHHEY  P G  + YAH AE+L LG  + +GPA+   H
Sbjct: 123 DFVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPH 182

Query: 199 MITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFT 258
           +IT WLW+ LR +E ++ H GY FPWSL+ F+P YGGAD HDYHH +      N++S F 
Sbjct: 183 LITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFV 242

Query: 259 YCDYIYGTDKGYRYQKKI 276
           Y D+I+GTDKGYR  K +
Sbjct: 243 YMDWIFGTDKGYRRLKTL 257

The following BLAST results are available for this feature:

BLAST of CSPI03G03210 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q1EC69	8.6e-133	74.83	Methylsterol monooxygenase 1-2 OS=Arabidopsis thaliana OX=3702 GN=SMO1-2 PE=2 SV...	[more]
Q8L7W5	2.9e-128	72.76	Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana OX=3702 GN=SMO1-1 PE=1 SV...	[more]
F4JLZ6	6.0e-118	67.48	Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana OX=3702 GN=SMO1-3 PE=2 SV...	[more]
Q9AV39	1.3e-117	70.00	Very-long-chain aldehyde decarbonylase GL1-10 OS=Oryza sativa subsp. japonica OX...	[more]
Q8VWZ8	1.3e-56	43.80	Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana OX=3702 GN=SMO2-2 PE=2 SV...	[more]

BLAST of CSPI03G03210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L5P8	5.0e-176	100.00	Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A1S3BKU6	1.2e-172	98.67	methylsterol monooxygenase 1-2-like OS=Cucumis melo OX=3656 GN=LOC103490939 PE=3...	[more]
A0A6J1E9U9	8.0e-166	94.00	methylsterol monooxygenase 1-2 OS=Cucurbita moschata OX=3662 GN=LOC111431186 PE=...	[more]
A0A6J1HQ57	1.8e-165	93.67	methylsterol monooxygenase 1-2 OS=Cucurbita maxima OX=3661 GN=LOC111465081 PE=3 ...	[more]
A0A6J1FUF2	2.2e-160	91.33	methylsterol monooxygenase 1-1-like OS=Cucurbita moschata OX=3662 GN=LOC11144822...	[more]

BLAST of CSPI03G03210 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650426.1	1.0e-175	100.00	methylsterol monooxygenase 1-2 [Cucumis sativus] >KGN55922.1 hypothetical protei...	[more]
XP_008448914.1	2.4e-172	98.67	PREDICTED: methylsterol monooxygenase 1-2-like [Cucumis melo]	[more]
XP_038876881.1	7.9e-168	95.00	methylsterol monooxygenase 1-2-like [Benincasa hispida]	[more]
XP_023552163.1	7.4e-166	94.33	methylsterol monooxygenase 1-2 [Cucurbita pepo subsp. pepo]	[more]
XP_022923513.1	1.6e-165	94.00	methylsterol monooxygenase 1-2 [Cucurbita moschata] >KAG6577591.1 Methylsterol m...	[more]

BLAST of CSPI03G03210 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G22756.1	6.1e-134	74.83	sterol C4-methyl oxidase 1-2	[more]
AT4G12110.1	2.0e-129	72.76	sterol-4alpha-methyl oxidase 1-1	[more]
AT4G22753.2	2.5e-119	66.55	sterol 4-alpha methyl oxidase 1-3	[more]
AT4G22753.1	4.2e-119	67.48	sterol 4-alpha methyl oxidase 1-3	[more]
AT1G07420.1	9.3e-58	43.80	sterol 4-alpha-methyl-oxidase 2-1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 269..296
None	No IPR available	PANTHER	PTHR11863	STEROL DESATURASE	coord: 1..287
None	No IPR available	PANTHER	PTHR11863:SF197	METHYLSTEROL MONOOXYGENASE 1-2	coord: 1..287
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 130..265 e-value: 3.8E-29 score: 101.7

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G03210.1	CSPI03G03210.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016126	sterol biosynthetic process
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0000254	C-4 methylsterol oxidase activity
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity