CSPI03G02680 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G02680
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionAsparagine--tRNA ligase
LocationChr3: 2051966 .. 2062070 (-)
RNA-Seq ExpressionCSPI03G02680
SyntenyCSPI03G02680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTAGAGGAATGAAAGGATCCAATTTTTTTAAGCTTTAGCAACCATACACGCCCGCGGCGACGGCACTAGCAATCAGACGGTCTTCGGGTCAGCTCCACCATTGAATGGATATTTACATAACCACCAGGAGTCACACACACACTCACACTAACAAAACAAGCTTTCTTCAATGGCTGCCGTTATCTCCCCGGCCACTTCTCTCCGCTTCAATCCTTGTTCAACTCTGCGTTTCATCTCCGGATTGCCAAGAAAACTTCCACACCACATTCTTTTCCCGCCAATTTTCCCCCGCCACTCTTCATGTTATTTCCGGCGAAGTTTCTGCTCTGCTGTCTCTGCCGGGGAAGCTGCGGAGAAGACGAAACCTGACGTTTCCAAAGATTGGAAGGTCACGCGTGGGGAGAAGGTTGGGGAATTCAGGAAGAAGCTGAGGATCTCTGAAATAAAGGGCGGCCGCGATGAAGGGCTGGATAGACTCGGGCAGACGTTTGTTGTTATGGGATGGGTTCGGACTCTTCGAGTTCAGAGCAGTGTTACGTTCATGGAGGTAAGATGAACTAATTCGTGACGTGAGTCTGTTTTACGTCTGAGAAATGTAGGATAGCAAGTTCGGTATATTCATATCTTCTTAAATTTTCGAATCATATGTATTCTGGTGGTGAATTCTGAATGAAATTGAAGGATAAGATTGCATTAATCATGTGAAACTGGTTCGATATTTTTGGGCTATTCTGGTATTCTTAGCGTTTTAAGCAAAAAAAACTCAGCTTCGTTGATGAAAAATTCAATAGTGCCATGGAGTAAGTGCCTGCTGTTGATTTAGTGGTAGTTTTGTACAAAGCTCCGTTTTTTTCTCTGCAAATTTTATTAGTTTTAGCAATGATGAACTGAATAGGTTGAGAATACCAATAGAGCCATCATCGACATTAATTTCAAATGGGGGCAGAAAAGAAAAGAAAAGAGAGAAGAATTTTGAAATTGGAATATTAGACAAAGGATTGAATATAGATTGTTGTAAGAAGTACCACTGTTATCTAAATGCCTCGCTTTGTTTTGTGCTTAGGTTAATGATGGATCATGCATTTCAAATATGCAATGTGTAATTGACTCAACGACAGAAGGTTATGATCAGGTACCCAAGTCAGCTCATTGTTGTACTCTTTTTTCTGTTTTCGAACAAGTGATTTTGATGGCAAGCATATCCATATTTTGATACAAAACTGTCCGGAAGACATTGAACATCAAATTGAAATGAGATAAAATAATACACGGGAAGACTGGATAACTGAATTGGGTTAGAAATATGCGTGTTTTGGTTATACCACCTACTTGCTTCATTGATTGTGTGATCATCCTCTTCTTTTTTATTGATATTCTTGGTGGATGATCATGAAAGCTGTTATCTAACCGTTGGCAGTAGAGGATGAAGTTATTTAAATTTATAGAGGATTGATTTGTTTTCCTTGAGACAAACTTCTTTTGGAAAAATTGGAAAAGTACAGATCTACAGGAGTTTGTATGAACTGTTTCTTCCTCTCGTTGGATTCTTTTAGTTGAGATCGGTAGTAAGGATTAGAAACAAAGGCACCATTAACTGTTGCAGACAGATAACGGGAAAAAAACCACGGACTCCAAGAGATGCATTAATTATGATATCTACTGGTCTCTTGTTCTTATTTCAGATTTGGAGTTTGTTGGCAGGTTGAATCTGGCTTGATCACCACTGGTGCATCAGTATGGGTGGAAGGGGTTGTGGTGGCCAGCCAAGGATCAAAACAGAAAGTGGAACTGAAACTCCAAAAAGTTATTGTGGTTAGTACTTCTTGTTCTTTTTGCTTAATTGGGTTAAATTGTTTTTCCCCGTGATCATCAACTGCAGTATTTGCCCATCATATAGGATTTGCATCCAGAAAAGGTTGATATATAATTTGAACAAAAGAAAATGATGCATATACATAACTTGTAATTATTTGTTCATTTTGCTTTTTTAAGTTTCTAAGATGAGGGATTCGTGAGTTGGATTATTTTCTTTGTAAAGGCAGTGACCATATGCATATGGGTACAAGAGGAAGCTAGAAAGAATCAAACAATTGCTAAGAGAGGGTCCATTGGGTAACCCTTTCTATTTTTGGCTCACACTTTCACGTAAGAAAAGTATTGAATTTTTTGTTTTGTGTGAAATTCGGGAACTCAACAAGTAAAGGAGGGTCTGTTTGGATTTTCTAATGAACAGGCAAGTGTGTAAAAGGATATTGTGTTTTAAGAAAGAAAAACAGGAGAAAACATGTATGAAGATGCAGTAGTGAGAAAAAGTATGCACACAGAAGAGGAGAGTCGAGAATGTGCACGAGATTGGTGAAAGAATGTGTGAGTTGAGAGAAGTTAATAAGAGAAAATATGTGGGTAAAGAGAGATCACTAAGAAAAAGACTGCATTGAGAAAATTTTATTATGAGTATATGCAATAATTAGCTATTATTTTATTAAAAAAGATGCAATAATTAGCTATTAGGAGCTTTCTTCGAAAAACTTGGCTAGACTCAAAGTCAAGGAGAAAAGGCACACCTACTTCTTATGCTATTTTGTACACAGCAGCCCCTTGGAGCAAATTAGAAATTTTTTTATTGTAAATACGCATTTAGAGATACTTCTAGTGGGAAGGATCTTCGTAACCCTGCTAGCCTTTTGTAAATTTCCTTGCATTAATAAAATTAGATTCTTTTTCCCTTAAAAAAGGCTAAGATATCGAATATATTCTTCAAAAACATTTTTGAAGAACAAAAAAAAAAAAATTGTGAGCAAACATATTTGATTTAAGATAAGTGAAAGAAGGAAATCTTGGACGCTTGCAGCAATAACTTGCTTCCTACTTTTGTCAGTAGATAAAAATAGGAAACACATTTTCCTTAAGTGAGGTAAAAGTTGAGATACTGAATCTACTTAGATTCATGCTCTACTCATGGCTGATAAATTATATTTTGTATCACGTACTTTATAGATTACTTGGAACCCATTCATTTAGTTGGGATGATGGGCTTGTTTTTTTGTATGCCCTTGTGTTCTTTTATTTTTCTCAATGAAAGTAGTTGTTTTTGTATTGCATGATCGAATGATCAAATTCAGAAAACATTTCTTCTCTACTTGTGTTAGTATTAATATTTGCTATATTTCAGGTTGGCAAGAGTGATCCTTCATTTCCTGTCCAAAAGAAAAAAGTTAGCAGAGAATTTTTGAGAACTAAAGCTCATCTTCGTCCAAGAACAAACACTTTTGGGGCGGTATAGTTCTATTTTTCATTCAGTTTAGTTTCCAGAGTTCAAACCAAGGGTGATAATCTTTTATATTCATGAAAAATAAAGGTCTTAATTGTCTAATTCACTAAAACGAACTATGCCATCATGAATAATTTAGGGATGAATGATGGATCAAGTGTTAAATGTCTCAACCGTCTTTTTTTGTGTGGATGGATTCACTCTGTGGATATAGATTTAGAAGGAAAAGTAAAATACTGGATGAATTCTGTGAAAGCAATTGTACGGAGTTTGTGTTTGGAAATGACATATTTAATAAAAGGAAGGAAAGTGGATGAGGTCTTTGGTTTTCGAATCAATGCACTTTTTGTCTAATTAGCTCAAATTGAAGTCCCTTGTTTTTCTTTTCCCTCCAATTGACCCAAGATTGGTTAATAATAAAAAAAAAGTTTAGGTTATGTTTTCCCTCAGGGGGGAAGAATTTCTCAAGAACACCTTGACATAATCTAAGGTTTTTGTACTTCATCTTTTAATTTTGCTTTTTCAACCTTTGGTGGTAACTATTTATGGCTTTCCTCTATACTAGTCAATTACCACTTTCTTGTTTCCCAGTAATTAGTAACTGATCAAAATAACTAGTTACATTAATTTCTGTACTTGCTTTTCTTTAGTAATTTCATATGAACAGTTGCCTAATTGTCAGTTTGAATTTGATGGTGTTGTGTAGGTTGCAAGGGTGAGAAATGCTTTGGCATATGCTACGCACAAGTTCTTCCAAGAAAATGGATTTGTTTGGGTTTCAAGTCCTATTATTACGGCATCAGATTGTGAAGGAGCTGGTGAACAGTTCTGTGTGACTACTTTGGTGGGTGTTTTAACTTAAAAGTCCTGGGATTTGTATGGTCTCTGCAATATATTTGTAGAACAATAGATCATTTTAAAATGAATAAAAAAAATTCTGATGTTGACAGATTCCAAGCTCTGGAGAACCTGCCAGTTCTGATGTGGGCTCTATTCCAAAGACAAAGGAGGGATTTATTGATTGGTCACAGGTGTGAATTAGCTTTGCTAACTTAGGAATCTAGCTCTTGGACTAGTTATATCATGTGCAACCATCTTTTTGAATCTTATGGAAGAAAATTGACGAGTCTGGATCAGACAAGCTCTTTGGCACTTCACCTGATTGTTTTTTTGCAAGTACTCATTAATTGTTTTTTTTTCCATTTCTGTGTTAAGGATTTTTTTGGGAAACCAGCATTCTTGACTGTCTCAGGGCAACTCAACGCTGAAACTTATGCTACTGCTCTCTCTGATGTACGGTTTTTCTTCCACACTGGTTCTTGCTTAGGCACGATGGTGGAACTTTTACAAGCTGCTAATAATTTCTTCCAAAATCTTTCTCAGGTTTATACTTTTGGGCCGACATTTCGAGCAGAAAATTCCAATACATCTAGGCATTTAGCTGAATTTTGGGTGAGTAGTATTCACATTTTGTTTAATTTCCTACCAAGTTCAGTTCTACAGTTTTCATATGTAAGAAGCACATATATATATATATATTTTGAAAAAAAAACCTTCTACTTTGTTCTTTAGATGATTGAACCGGAACTCGCATTTGCTGATTTGGATGACGACATGTCTTGTGCAACTGCATATCTCCAGTATGTAGTATGTTACCTTTCATGACTATTGTGTTGTCTTACACCATACTTGATTTTAAAATGTTTGGAATAAAAGTTTCTATATTTTTTTACTTGACTAAAGGTTAACAAAAGCTTTTAATTCTAGGTGAGACATATTCTTGAAAATTGCAAGGAAGACTTGGATTTCTTCAATACGTGGATCGAGAAAGGAATTATTGATCGACTGACTGTAGGAAATCTTACCATTACCCCTCGTCTGTTATACATCTATTATTCCTTTAAATTTTAGTTGATATTGTGTATCCGAAAATTTATTTGCATGATATATATCATCTATGCCAACTTACCATCGCAATAACTTGTGCGGTGTATTCTGCAGAACCTGGTTGAAAATGATTTTGTGCAGTTGCCTTACACTGATGCAATTGAGCTTCTTCTTCGAGCAAATAAGAAGTTTGAATATCCGGTAATTGCCATTAGATTTATCACACCAAGCTATAGAAGTTTTGGTTCCATCTTGATTTTGTTCTCTGTTATTCTGCTTGTTTCATATTGGTAACGTATTTAAACAGAACCAGTTTACTTACTTAGATAGGAACGTTCATACATTGCGAAAAGATAATTCTAGAAATCCCTAATTCGTCTCTCGACTCCAAATCTAATAACAATTTAAAGAGCACCCTCTCTCAGCCACGGTTACCTTCAGAACACTAACCGAGGTCACTTTTTCATTTTTCTTCCAAGTATTGTTGGCTACAGCGGATCCTACACTCATTCTAACTGCTTAGCACATACATCCTCCCAAAACCACACAGCACACACTTTCTTGATCGATGGCCCATCAAATTCTGTACTGGAAAGAAACTGTTCAATGGTATATTTGTTTTGTTTGCTTCTTATTAATCTAGTTTTGGTTTGTCACTTACTATTGCATTGGTACTCGAGTCAAGTTGCTTTCATCAATGTTGTCACTTATTTTTGCCTCTCAAGTTATTGTAATACTATCTTGACATTGCCAAGTTTGTGCATTTATTTTTCTCTCTTCCCAGAAGTGTCATCAGATGTAAGCGTCCTTTTATTGATCACGTTGAACACTTTCTTAGGATTCTCGGTCTTACCATTGGTGCTAATAAGTCAAATGCCCAAGTATACTATTTGCTGTGCATTGCTGTGTGGATCTATAATTTGGTCATTGCTGTGTGGATCTCAAACTTTTTAAATTATTTTTAGGTCAAGTGGGGAAGTGATTTGCAAAGTGAACACGAGCGTTATATAACTGAGGTGGCATTTGGTGGATGCCCTGTCATAATTAGAGACTATCCAAGGGTATGGTTTTCTTTTTGTTTCTAATCTTTTGAATATTGATAAAAGAAATTACACTGATTTAGTCAATTGACGTGAAATTATAGGATATAAAAGCATTCTACATGCGGCAAAATGATGACGGGAAGACTGTTGCAGCCATGGATATGTTGGTTCCAAAGGTAATGTCATGTTTTGTTTTTGTTTTTGTTTTTCAATTACATTTCATTAAGAAAAAGTTAAAGCATACAAGGACATAAAAAAAAACCAAGTTAACAAGTCCCTGTTATAGCTTATAAGCAACTGTTAACCTATATCCTTGTCAAAAAAAAAGAAAAGAAAAGAAAAGAACTTGTTAAGCTAAATCAATCGGTAAGGGTGGATAATTTATAATTATAAAAATGGGGACTCAGTTATGTAATTGATACGTGAAGCGCTTGGGAATCTGACTCCATATCCCAAAAGATATGAAAGTTACAGTCCAACCAAATGAACTACAAAATGGGCCAAATGAAATGAAGATAAAGGATTTTCCTTTCTTTCTTGAACGATTTCATATTGAATTAAAGAGAAAGGAGGTTCCAAATGTCAAAAGGTAAGACAAGCTTCCATTTAAAGGCAGCAAGCAAATGCTCCTTTATAAATCTGGACTGATTACACCAAATGAAAAGGTGGTTATGTGATTTCCCCAGAATTTTTTACATAAGATGCGGCAACTAGGAAAGATGCAAATCCAAGGAGATCAACATTGAATTCTGTCTGCGTATTCAAGCAAGAGTGACTGAGCTCCCATGCATAAAAGACCTTGGGGTATTTTCCAATACAGATGGAGAGACGATGATCTTTACTGCCAATTTCACCAACCTATGATAATTACTTGAAAAGAGATTTTGACGTAAAGACCCCCTCCTTTTCCAAGCTCCAAAATGATCCTTAGAATGTGTTTAGAATGATTTTTTAAGTGCTTAAAAAAGTGTTGTAAAAAAAGTTTTTGATAGCTGGTGTGGTATCGAGGTAAATCACAGCCAGTGAGTTTAATAGATTGGCCCAATCCTCAATTACTCATTTACTCTATCTGTCAGATTTCTCCTTGGACAAAAATTCCAGGATGCTGTTGATGAATTCTTCATGCCAGCCACAGTCGTTTCTTTTTAGTTATGGCAAACAGGGAGGGAATCAATCTTTTAGGGCCTTATTTACCTGTTAAGTATCAGACAAATATCGTGTGTCGACACCGTTTCCTACTTCACAGGATATGTGTTCATAAACAGAATCTTTGAATTTCTCTATGTACTTCCAACTTTTCAAGGTCTTTTGAGGTGCTTAGATAATAAGTTCCCGGTTTGAGTTCTTGACGATGAGACCAATATGGCAAAACAACTTTCCTCCAAAGAGCAAACATTTCTATGTGAAATCTCCAATTCCACTTTGTCGAAATTGATAAAATTTTCAGCTGTAAGTCATTAATCCCAAGTCCTCCTTTCTTGGGGTTCCCTCGGGTAATGTTAAAGTTATACTTTTCTCGGATTATCTTAACAATTTTTTTATATGAAATTGTGTGGTACAAGTGTAGGTATAACTAATTTAGTAGTTTTCCAAGAGCATGGCTATAAATACACAATTAAATATTGATAGTTTATTTTTCTCGTGATAACTTCTATTATACGAGAGGAACTTCTAACCTTGGTTATTGTTAAAGTGAAGGTATCATATAAAGTTGTTTTCTTTTTTCCCTCCTCGCTCTCTCTTCAGATTGGGGAGCTTATAGGTGGAAGTCAAAGAGAAGAACGGCTTGATTATTTGGAAGCTCGGCTGGATGACTTGAAACTCAATAAAGAAAGCTACTGGTGGTACCTTGACTTGCGTCGTTATGGTTCAGGTTAGAGCTGAAGCCCTTAGAGTTATGATACTGAGTTTGTTTTGCGATTAAAAAGAAGAAAAAATATGTTTCAATTTTATCAGACGTCCCCGAAGGGACTAAAGAGAAGAAAATTATGAAAGAAGAAAATCTTATAACTACATAGAATAATGACGTTTTTTCCATTTTTCAATTTGAAGACAAATAGAAGTTAAAGACGGCCAATTAAAACACAGAACCACCCTAACAAAGAAGTGAACGAAACATAACAAAAGCAAATTACAAAAGTTGCTTATGAATTAAAAATAAGAAGTAATTACAAACCCTTTTAGATAAGAATGCCTGGAGAGGCGTTGAAATTAAATGTGTCACCTTCGAAACCTTCTCTTAAGAACTCTCCAATTTCTCTTCACCTAAAATTTTAGAAGGTTGCTAAGAAAGGCAATTGCTAAAGAGTTGATCTCTTTCCTTGCATGTATCTCCGCCAACATATACTTGCAATTTCTGGTAGTACGGATCGAGAAGTAGGGACGAGTGGGACAGGATGAGGAATTAGATTGTCAAACTGACTTGGAAGAAGAAAAAATGCCTGAGGCCCCCCGAGAATTTGCCTACATGACCCATTGATATATGGAATGAAACAAACAGTGAGTAATTTAGTACCGAAAGGAAATCAGACTAATCATGAGATTCCACATCAAATCAAGAGGAAAGGAAAGCAATCATCAGTATAACATAAATGAATTAATCAATGCCTTCTATCCGAGGAAAAAATTAAAGAGGTGGGGAAGAAATGTGAAGGCAGTCATTACCTGTCAATGAAAATTTCCAGAACTTGGTTTTCAAGGACTTCTGGATATGCCTTTAAAGACCACAAACATGATCCACCCTAAGGCTTGACGACCATGACTTACTATGATCCTGTGATACAAAGATATTATTGGATGTCCCCCTAAAGGACGTGATACAAAGATATTAGGAATCAAACTTCAAGTGAGAAACGGTAAACTTGATGATTTGTAACGATCTGCTGAAATAAGGTAAAATGCTGAATCTTTCTTAAAGCAATTGAATCTTTTAAGCTATCAACTATCATCGTAATTTTGAGGATTCATCACATGAAAATCTTGTTAAAGCCATCTATTTGAGGATTACCGTATATTAGACAGGACTGCCTATTTCCTAAGGTAAAAGAGTAAAAAGAGATGAGATCTAAAGCCTAATCTTTTAGCATGATCGGTGATGAATCTATATAGATTATACTTGCAGAACTCCTAATCTACTTCGGAAGGAGAAAGATTTAGCATAAACATCTTTTAGCCTATAGATCAATGGAATTTTGATGATTGCATCAAAATTTTCAGATCTTGGCAAACTTAATACGATAATATGATTCCAAGCATATGCCATTATGTGACCTTTTGATGATGTTAACTAACTTTTTCTCGTGCCAACAAGTGCATTGCTCAACTAACATGATACATGTCCTCTTGACCAAGTGGTAAGAGGTTAACATATTCTAACTAACATACTGTTGAGCTTAAAGAGAAAACTCGTATTCAAATTTCTACATTCTCTAATCAAAGCTCTTGTGTTTCAGTTCCCCACGCAGGCTTTGGGTTGGGGTTCGAAAGACTTGTGCAGTTTGTCACTGGAATAGATAACATTAGAGATGCCATCCCATTTCCTCGTTCGCCTGGCTCTGCTGAATTTTAATGCCAAACCAAAATTGTTCATCAACAGACAACAATATCTTAATTTACTCATTCTTTTATTTAACATCCCGTCTTACTTTTTATAGAACTTGTTCTTTCTACTCTTCCTTCACCATTGTATTTTTGTTATGTTGACACTTAAAACTGCGCAAACACTCCCACCATAAGGGCTGAAAGTTTGAAATTATGTTGTATTCATTCTATACATTGTAATAAAGCATTCTTCATTTAGAACCAATTGGTTGGAACGAGTGCTTTATCTTTGCTAAACTGTTGTTGGAATCACCATTTTGATATGTATTTGTAAAATATTAACAAATGTTCTTGGTTGTGGT

mRNA sequence

AATTAGAGGAATGAAAGGATCCAATTTTTTTAAGCTTTAGCAACCATACACGCCCGCGGCGACGGCACTAGCAATCAGACGGTCTTCGGGTCAGCTCCACCATTGAATGGATATTTACATAACCACCAGGAGTCACACACACACTCACACTAACAAAACAAGCTTTCTTCAATGGCTGCCGTTATCTCCCCGGCCACTTCTCTCCGCTTCAATCCTTGTTCAACTCTGCGTTTCATCTCCGGATTGCCAAGAAAACTTCCACACCACATTCTTTTCCCGCCAATTTTCCCCCGCCACTCTTCATGTTATTTCCGGCGAAGTTTCTGCTCTGCTGTCTCTGCCGGGGAAGCTGCGGAGAAGACGAAACCTGACGTTTCCAAAGATTGGAAGGTCACGCGTGGGGAGAAGGTTGGGGAATTCAGGAAGAAGCTGAGGATCTCTGAAATAAAGGGCGGCCGCGATGAAGGGCTGGATAGACTCGGGCAGACGTTTGTTGTTATGGGATGGGTTCGGACTCTTCGAGTTCAGAGCAGTGTTACGTTCATGGAGGTTAATGATGGATCATGCATTTCAAATATGCAATGTGTAATTGACTCAACGACAGAAGGTTATGATCAGGTTGAATCTGGCTTGATCACCACTGGTGCATCAGTATGGGTGGAAGGGGTTGTGGTGGCCAGCCAAGGATCAAAACAGAAAGTGGAACTGAAACTCCAAAAAGTTATTGTGGTTGGCAAGAGTGATCCTTCATTTCCTGTCCAAAAGAAAAAAGTTAGCAGAGAATTTTTGAGAACTAAAGCTCATCTTCGTCCAAGAACAAACACTTTTGGGGCGGTTGCAAGGGTGAGAAATGCTTTGGCATATGCTACGCACAAGTTCTTCCAAGAAAATGGATTTGTTTGGGTTTCAAGTCCTATTATTACGGCATCAGATTGTGAAGGAGCTGGTGAACAGTTCTGTGTGACTACTTTGATTCCAAGCTCTGGAGAACCTGCCAGTTCTGATGTGGGCTCTATTCCAAAGACAAAGGAGGGATTTATTGATTGGTCACAGGATTTTTTTGGGAAACCAGCATTCTTGACTGTCTCAGGGCAACTCAACGCTGAAACTTATGCTACTGCTCTCTCTGATGTTTATACTTTTGGGCCGACATTTCGAGCAGAAAATTCCAATACATCTAGGCATTTAGCTGAATTTTGGATGATTGAACCGGAACTCGCATTTGCTGATTTGGATGACGACATGTCTTGTGCAACTGCATATCTCCAGTATGTAGTGAGACATATTCTTGAAAATTGCAAGGAAGACTTGGATTTCTTCAATACGTGGATCGAGAAAGGAATTATTGATCGACTGACTAACCTGGTTGAAAATGATTTTGTGCAGTTGCCTTACACTGATGCAATTGAGCTTCTTCTTCGAGCAAATAAGAAGTTTGAATATCCGGTCAAGTGGGGAAGTGATTTGCAAAGTGAACACGAGCGTTATATAACTGAGGTGGCATTTGGTGGATGCCCTGTCATAATTAGAGACTATCCAAGGGATATAAAAGCATTCTACATGCGGCAAAATGATGACGGGAAGACTGTTGCAGCCATGGATATGTTGGTTCCAAAGATTGGGGAGCTTATAGGTGGAAGTCAAAGAGAAGAACGGCTTGATTATTTGGAAGCTCGGCTGGATGACTTGAAACTCAATAAAGAAAGCTACTGGTGGTACCTTGACTTGCGTCGTTATGGTTCAGTTCCCCACGCAGGCTTTGGGTTGGGGTTCGAAAGACTTGTGCAGTTTGTCACTGGAATAGATAACATTAGAGATGCCATCCCATTTCCTCGTTCGCCTGGCTCTGCTGAATTTTAATGCCAAACCAAAATTGTTCATCAACAGACAACAATATCTTAATTTACTCATTCTTTTATTTAACATCCCGTCTTACTTTTTATAGAACTTGTTCTTTCTACTCTTCCTTCACCATTGTATTTTTGTTATGTTGACACTTAAAACTGCGCAAACACTCCCACCATAAGGGCTGAAAGTTTGAAATTATGTTGTATTCATTCTATACATTGTAATAAAGCATTCTTCATTTAGAACCAATTGGTTGGAACGAGTGCTTTATCTTTGCTAAACTGTTGTTGGAATCACCATTTTGATATGTATTTGTAAAATATTAACAAATGTTCTTGGTTGTGGT

Coding sequence (CDS)

ATGGCTGCCGTTATCTCCCCGGCCACTTCTCTCCGCTTCAATCCTTGTTCAACTCTGCGTTTCATCTCCGGATTGCCAAGAAAACTTCCACACCACATTCTTTTCCCGCCAATTTTCCCCCGCCACTCTTCATGTTATTTCCGGCGAAGTTTCTGCTCTGCTGTCTCTGCCGGGGAAGCTGCGGAGAAGACGAAACCTGACGTTTCCAAAGATTGGAAGGTCACGCGTGGGGAGAAGGTTGGGGAATTCAGGAAGAAGCTGAGGATCTCTGAAATAAAGGGCGGCCGCGATGAAGGGCTGGATAGACTCGGGCAGACGTTTGTTGTTATGGGATGGGTTCGGACTCTTCGAGTTCAGAGCAGTGTTACGTTCATGGAGGTTAATGATGGATCATGCATTTCAAATATGCAATGTGTAATTGACTCAACGACAGAAGGTTATGATCAGGTTGAATCTGGCTTGATCACCACTGGTGCATCAGTATGGGTGGAAGGGGTTGTGGTGGCCAGCCAAGGATCAAAACAGAAAGTGGAACTGAAACTCCAAAAAGTTATTGTGGTTGGCAAGAGTGATCCTTCATTTCCTGTCCAAAAGAAAAAAGTTAGCAGAGAATTTTTGAGAACTAAAGCTCATCTTCGTCCAAGAACAAACACTTTTGGGGCGGTTGCAAGGGTGAGAAATGCTTTGGCATATGCTACGCACAAGTTCTTCCAAGAAAATGGATTTGTTTGGGTTTCAAGTCCTATTATTACGGCATCAGATTGTGAAGGAGCTGGTGAACAGTTCTGTGTGACTACTTTGATTCCAAGCTCTGGAGAACCTGCCAGTTCTGATGTGGGCTCTATTCCAAAGACAAAGGAGGGATTTATTGATTGGTCACAGGATTTTTTTGGGAAACCAGCATTCTTGACTGTCTCAGGGCAACTCAACGCTGAAACTTATGCTACTGCTCTCTCTGATGTTTATACTTTTGGGCCGACATTTCGAGCAGAAAATTCCAATACATCTAGGCATTTAGCTGAATTTTGGATGATTGAACCGGAACTCGCATTTGCTGATTTGGATGACGACATGTCTTGTGCAACTGCATATCTCCAGTATGTAGTGAGACATATTCTTGAAAATTGCAAGGAAGACTTGGATTTCTTCAATACGTGGATCGAGAAAGGAATTATTGATCGACTGACTAACCTGGTTGAAAATGATTTTGTGCAGTTGCCTTACACTGATGCAATTGAGCTTCTTCTTCGAGCAAATAAGAAGTTTGAATATCCGGTCAAGTGGGGAAGTGATTTGCAAAGTGAACACGAGCGTTATATAACTGAGGTGGCATTTGGTGGATGCCCTGTCATAATTAGAGACTATCCAAGGGATATAAAAGCATTCTACATGCGGCAAAATGATGACGGGAAGACTGTTGCAGCCATGGATATGTTGGTTCCAAAGATTGGGGAGCTTATAGGTGGAAGTCAAAGAGAAGAACGGCTTGATTATTTGGAAGCTCGGCTGGATGACTTGAAACTCAATAAAGAAAGCTACTGGTGGTACCTTGACTTGCGTCGTTATGGTTCAGTTCCCCACGCAGGCTTTGGGTTGGGGTTCGAAAGACTTGTGCAGTTTGTCACTGGAATAGATAACATTAGAGATGCCATCCCATTTCCTCGTTCGCCTGGCTCTGCTGAATTTTAA

Protein sequence

MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPIFPRHSSCYFRRSFCSAVSAGEAAEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVVMGWVRTLRVQSSVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQKVELKLQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSCATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRANKKFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLVPKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF*
Homology
BLAST of CSPI03G02680 vs. ExPASy Swiss-Prot
Match: O48593 (Asparagine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SYNO PE=2 SV=3)

HSP 1 Score: 874.8 bits (2259), Expect = 5.3e-253
Identity = 432/572 (75.52%), Postives = 491/572 (85.84%), Query Frame = 0

Query: 1   MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPI----------FPRHSSCYFRRS 60
           MAA   PATSLR    STLRF+S      P + LF P+          FP +S    RR 
Sbjct: 1   MAATFLPATSLRLTQNSTLRFLSFFTISNPSYSLFRPLRRRVLPPFDAFPANSR---RRC 60

Query: 61  FCSAVSAGEAAEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVVM 120
           FC+AVS  E+      +  + ++   G KVGEFRKKLRI+E+KGG DEGL R+GQ+  +M
Sbjct: 61  FCTAVS--ESLGSGDGNKVESYEKRFGSKVGEFRKKLRIAEVKGGADEGLSRVGQSLNIM 120

Query: 121 GWVRTLRVQSSVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVAS 180
           GWVRTLR QSSVTF+E+NDGSC+SN+QCV+ S  EGYDQVESG I TGASV V+G +VAS
Sbjct: 121 GWVRTLRSQSSVTFIEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGASVSVQGTIVAS 180

Query: 181 QGSKQKVELKLQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALA 240
           QG+KQKVELK++K+IVVG+ D S+P+QKK+VSREFLRTKAHLRPRTNTFGAVARVRN LA
Sbjct: 181 QGTKQKVELKVEKIIVVGECDSSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTLA 240

Query: 241 YATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFI 300
           YATHKFFQE+GFVWV+SPIITASDCEGAGEQFCVTTLIPSS E   + + +IPKTK G I
Sbjct: 241 YATHKFFQESGFVWVASPIITASDCEGAGEQFCVTTLIPSSHENTDTSIDAIPKTKGGLI 300

Query: 301 DWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELA 360
           DWSQDFFGKPAFLTVSGQLN ETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELA
Sbjct: 301 DWSQDFFGKPAFLTVSGQLNGETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELA 360

Query: 361 FADLDDDMSCATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTD 420
           FADLDDDM+CATAYLQYVV+++L+NCKED++FF+TWIEKGII RL+++ E +F+QL YTD
Sbjct: 361 FADLDDDMACATAYLQYVVKYVLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTD 420

Query: 421 AIELLLRANKKFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDG 480
           AIE+LL+ANKKF++PVKWG DLQSEHERYITE AFGG PVIIRDYP++IKAFYMR+NDDG
Sbjct: 421 AIEILLKANKKFDFPVKWGLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDDG 480

Query: 481 KTVAAMDMLVPKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGF 540
           KTVAAMDMLVP+IGELIGGSQREERL+ LEARLD+LKLNKESYWWYLDLRRYGSVPHAGF
Sbjct: 481 KTVAAMDMLVPRIGELIGGSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGF 540

Query: 541 GLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 563
           GLGFERLVQFVTGIDNIRD IPFPR+P SAEF
Sbjct: 541 GLGFERLVQFVTGIDNIRDVIPFPRTPASAEF 567

BLAST of CSPI03G02680 vs. ExPASy Swiss-Prot
Match: Q6MEC9 (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 2.7e-164
Identity = 277/478 (57.95%), Postives = 360/478 (75.31%), Query Frame = 0

Query: 86  KLRISEIKGGRDEGLDRLGQTFVVMGWVRTLRVQSSVTFMEVNDGSCISNMQCVIDSTTE 145
           + +I  ++G   E    +G    + GWVRT+R Q + TF+E+NDGS +SN Q +      
Sbjct: 2   RTKIKSLRGTTPEVRALIGHDITLKGWVRTVRNQKTFTFIEINDGSTLSNFQIIATPDIA 61

Query: 146 GYDQVESGLITTGASVWVEGVVVASQGSKQKVELKLQKVIVVGKSDPS-FPVQKKKVSRE 205
           GYDQ+ + L +TG SV   G +V S G +Q +E++   + ++GK DP  + +QKK+ + E
Sbjct: 62  GYDQLINQL-STGVSVSAIGTIVESPGKEQNLEMQATAITIIGKCDPEVYLLQKKRHTFE 121

Query: 206 FLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQENGFVWVSSPIITASDCEGAGEQFCV 265
           FLR+ AHLRPRTNT GAV RVRNALA+ATH+FFQ+ GF+++ +PIIT SDCEGAG+ F V
Sbjct: 122 FLRSIAHLRPRTNTIGAVTRVRNALAFATHQFFQKRGFLYIHTPIITGSDCEGAGKMFQV 181

Query: 266 TTLIPSSGEPASSDVGSIPKTKEGFIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTF 325
           TTL          D  +  +T EG +D++QDFFGKP +LTVSGQLN E YA ALSDVYTF
Sbjct: 182 TTL----------DQNNPARTPEGRVDYTQDFFGKPTYLTVSGQLNGEIYACALSDVYTF 241

Query: 326 GPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSCATAYLQYVVRHILENCKEDLDFFN 385
           GPTFRAENSNTSRHLAEFWMIEPE+AFADL+D+M CA  YL+Y+++++L+NC+ED++FFN
Sbjct: 242 GPTFRAENSNTSRHLAEFWMIEPEMAFADLNDNMDCAEDYLKYILKYVLDNCQEDMEFFN 301

Query: 386 TWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRANKKFEYPVKWGSDLQSEHERYITEVA 445
             +   +I RL +++   F +  YT A+ +L +A+KKFEYPVKWG DLQSEHER++ E  
Sbjct: 302 KHVATDLISRLEHVINTSFERASYTYAVRILEKADKKFEYPVKWGLDLQSEHERFLAEEF 361

Query: 446 FGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLVPKIGELIGGSQREERLDYLEARLD 505
           FG  PVI+ DYP+DIKAFYMR N+D KTVAAMD+LVPK+GE+IGGSQREERL  LE++L 
Sbjct: 362 FGK-PVILTDYPKDIKAFYMRTNEDNKTVAAMDVLVPKVGEIIGGSQREERLSVLESKLK 421

Query: 506 DLKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 563
           +  L  E YWWYL+LR++GSVPH+GFG GFERLVQF TG++NIRD IPFPR PG A+F
Sbjct: 422 EFNLPAEEYWWYLELRKFGSVPHSGFGAGFERLVQFTTGMENIRDVIPFPRHPGKADF 467

BLAST of CSPI03G02680 vs. ExPASy Swiss-Prot
Match: P58692 (Asparagine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=asnS PE=3 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 3.8e-158
Identity = 288/478 (60.25%), Postives = 347/478 (72.59%), Query Frame = 0

Query: 88  RISEI--KGGRDEGLDRLGQTFVVMGWVRTLRVQSSVTFMEVNDGSCISNMQCVIDSTTE 147
           RI+EI   G  DE L       VV GWVRT R      F+EVNDGS + N+Q VI+    
Sbjct: 5   RIAEILRSGQPDESL-------VVQGWVRTKRELKGFAFIEVNDGSSLGNLQVVINQDLP 64

Query: 148 GYDQVESGLITTGASVWVEGVVVASQGSKQKVELKLQKVIVVGKSDP-SFPVQKKKVSRE 207
            Y  +   L  TGASV V GV+VASQG  Q++ELK + V V G++DP ++P+QKK+ S E
Sbjct: 65  DYAVIVKQL-NTGASVEVNGVLVASQGKGQRIELKAEAVKVYGEADPETYPLQKKRHSFE 124

Query: 208 FLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQENGFVWVSSPIITASDCEGAGEQFCV 267
           FLRT  HLR RTN+FGAV RVRNA + A H+FFQE GF+WV +PIITASDCEGAGE F V
Sbjct: 125 FLRTIGHLRSRTNSFGAVFRVRNACSAAIHQFFQERGFLWVHTPIITASDCEGAGELFSV 184

Query: 268 TTLIPSSGEPASSDVGSIPKTKEGFIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTF 327
           T+L          D+  IP+T+   ID+SQDFF KP +LTVSGQL AE  A A S+VYTF
Sbjct: 185 TSL----------DLKQIPRTENQGIDYSQDFFAKPTYLTVSGQLEAEVMAMAFSNVYTF 244

Query: 328 GPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSCATAYLQYVVRHILENCKEDLDFFN 387
           GPTFRAENSNTSRHLAEFWM+EPE+AF DL+ DM  A A+L+++  H+LE C ED++FFN
Sbjct: 245 GPTFRAENSNTSRHLAEFWMVEPEMAFCDLEGDMDLAEAFLKHIFNHVLEKCPEDMEFFN 304

Query: 388 TWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRANKKFEYPVKWGSDLQSEHERYITEVA 447
             I+  ++    N++ N F +L YTDAI+LL +A+ KFEYPV WG DLQSEHERY+ E  
Sbjct: 305 QRIDNTVLATAENIINNQFERLTYTDAIKLLEKADVKFEYPVSWGLDLQSEHERYLAEQL 364

Query: 448 FGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLVPKIGELIGGSQREERLDYLEARLD 507
           F   PVI+ DYP  IKAFYMR +DD KTV AMD+L PKIGE+IGGSQREERLD LE R+ 
Sbjct: 365 FKK-PVIVTDYPAQIKAFYMRLSDDEKTVRAMDVLAPKIGEIIGGSQREERLDVLERRVL 424

Query: 508 DLKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 563
              +  E  WWYLDLRRYG+VPHAGFGLGFERLVQF+TG+ NIRD IPFPR+P +AEF
Sbjct: 425 AQGMQPEDLWWYLDLRRYGTVPHAGFGLGFERLVQFITGMGNIRDVIPFPRTPQNAEF 463

BLAST of CSPI03G02680 vs. ExPASy Swiss-Prot
Match: P52276 (Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=asnS PE=3 SV=2)

HSP 1 Score: 556.6 bits (1433), Expect = 3.2e-157
Identity = 276/456 (60.53%), Postives = 344/456 (75.44%), Query Frame = 0

Query: 108 VVMGWVRTLRVQSSVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVV 167
           +V GW+RT R     +F+EVNDGS ++N+Q V+D +   YD++ S L  TGA++ VEG +
Sbjct: 20  MVQGWLRTKRTLKDFSFVEVNDGSSLANLQVVLDGSLADYDRLLSQL-QTGAALVVEGKL 79

Query: 168 VASQGSKQKVELKLQKVIVVGKSDP-SFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVR 227
             S G  Q+VELK  K+ ++G +DP S+P+QKK+ S EFLRT  HLRPRTNT GAV RVR
Sbjct: 80  APSPGKGQRVELKATKLELLGGADPGSYPLQKKRHSFEFLRTIGHLRPRTNTIGAVMRVR 139

Query: 228 NALAYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTK 287
           NA A A H+FFQE GF+WV +PIITASDCEGAG+ F VTTL          D+  +PK  
Sbjct: 140 NACATAIHQFFQERGFLWVHTPIITASDCEGAGDLFKVTTL----------DLQRVPKNG 199

Query: 288 EGFIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIE 347
           EG ID+SQDFFGK A+LTVSGQL AE  A A  +VYTFGPTFRAENSNTSRHLAEFWM+E
Sbjct: 200 EG-IDYSQDFFGKQAYLTVSGQLEAEVMALAFQNVYTFGPTFRAENSNTSRHLAEFWMVE 259

Query: 348 PELAFADLDDDMSCATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQL 407
           PE+AF DL+ D   A  +L+Y+ + +LE C ED++FF+  I+  ++    N++ N+F  L
Sbjct: 260 PEMAFCDLEGDRQWAEEFLKYIFKFVLEKCPEDMEFFDQRIDNTVLATADNIINNEFAWL 319

Query: 408 PYTDAIELLLRANKKFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQ 467
            Y++AI+LL +A++KFEYPV WG DLQSEHERY+ E  F   P I+ DYP+DIKAFYMR 
Sbjct: 320 TYSEAIKLLEKADQKFEYPVAWGVDLQSEHERYLAETVFKR-PTIVTDYPKDIKAFYMRL 379

Query: 468 NDDGKTVAAMDMLVPKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVP 527
           NDDGKTVAAMD+L PKIGE+IGGSQREERLD L  R+ +  + +E  WWYLDLRRYGSVP
Sbjct: 380 NDDGKTVAAMDILAPKIGEIIGGSQREERLDILTQRMQEQGVPEEDLWWYLDLRRYGSVP 439

Query: 528 HAGFGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 563
           HAGFGLGFER+VQF+TG+ NIRD IPFPR+P +AEF
Sbjct: 440 HAGFGLGFERIVQFMTGMANIRDVIPFPRTPMNAEF 462

BLAST of CSPI03G02680 vs. ExPASy Swiss-Prot
Match: Q899M9 (Asparagine--tRNA ligase OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=asnS PE=3 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 7.9e-156
Identity = 264/460 (57.39%), Postives = 347/460 (75.43%), Query Frame = 0

Query: 103 LGQTFVVMGWVRTLRVQSSVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVW 162
           + +  VV GW+RT R   +  F+E+NDGS   N+Q +ID+  + +D+V    +   +S+ 
Sbjct: 17  INKDIVVAGWIRTERTSKNFGFIELNDGSFFKNLQIIIDTNLDNFDKVSK--LPISSSIV 76

Query: 163 VEGVVVASQGSKQKVELKLQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAV 222
           VEG +VA+  +KQ  E++  KV V G S   +P+QKK+ S EFLR+ AHLRPR+NTF AV
Sbjct: 77  VEGKLVATPSAKQPFEVQASKVTVEGTSLQDYPLQKKRHSFEFLRSIAHLRPRSNTFSAV 136

Query: 223 ARVRNALAYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSI 282
            R+R+  AYA HKFFQE  FV+V +PIITASDCEGAGE F VTTL          D+ +I
Sbjct: 137 FRIRSLAAYAVHKFFQEKNFVYVHTPIITASDCEGAGEMFRVTTL----------DLNNI 196

Query: 283 PKTKEGFIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEF 342
           PK  EG ID+SQDFF K A LTVSGQL+AE+YA A  +VYTFGPTFRAENSNT+RH +EF
Sbjct: 197 PKNDEGKIDFSQDFFDKDAKLTVSGQLSAESYALAFGNVYTFGPTFRAENSNTARHASEF 256

Query: 343 WMIEPELAFADLDDDMSCATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVEND 402
           WMIEPE+AFADL D M  A   ++Y++ ++LEN  E+++FFN++I+K +  RL N+V ++
Sbjct: 257 WMIEPEMAFADLTDYMDVAEEMVKYIINYVLENAPEEMNFFNSFIDKDLFKRLDNVVNSE 316

Query: 403 FVQLPYTDAIELLLRANKKFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAF 462
           F ++ YT+A++LL ++ +KF+YPV+WG DLQ+EHERYITE  FG  PV + DYP+DIKAF
Sbjct: 317 FKRITYTEAVDLLQKSGEKFQYPVEWGIDLQTEHERYITEKIFGK-PVFVTDYPKDIKAF 376

Query: 463 YMRQNDDGKTVAAMDMLVPKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRY 522
           YMR N+D KTVAA D+LVP +GE+IGGSQREERLD LE R+ +  LN++ YWWYL+LR+Y
Sbjct: 377 YMRLNEDNKTVAAADLLVPGVGEIIGGSQREERLDVLEERMKEFDLNEKDYWWYLELRKY 436

Query: 523 GSVPHAGFGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 563
           GS  H+GFGLGFER++ ++TGI NIRD IPFPR+PGSAEF
Sbjct: 437 GSAKHSGFGLGFERILMYMTGISNIRDVIPFPRTPGSAEF 463

BLAST of CSPI03G02680 vs. ExPASy TrEMBL
Match: A0A0A0L5I1 (Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_3G020020 PE=3 SV=1)

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 560/562 (99.64%), Postives = 561/562 (99.82%), Query Frame = 0

Query: 1   MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPIFPRHSSCYFRRSFCSAVSAGEA 60
           MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPIFPRHSSCYFRRSFCSAVSAGEA
Sbjct: 1   MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPIFPRHSSCYFRRSFCSAVSAGEA 60

Query: 61  AEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVVMGWVRTLRVQS 120
           AEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEG+DRLGQTFVVMGWVRTLRVQS
Sbjct: 61  AEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGVDRLGQTFVVMGWVRTLRVQS 120

Query: 121 SVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQKVELK 180
           SVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQKVELK
Sbjct: 121 SVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQKVELK 180

Query: 181 LQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQEN 240
           LQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQEN
Sbjct: 181 LQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQEN 240

Query: 241 GFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFIDWSQDFFGKP 300
           GFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPA SDVGSIPKTKEGFIDWSQDFFGKP
Sbjct: 241 GFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPARSDVGSIPKTKEGFIDWSQDFFGKP 300

Query: 301 AFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSC 360
           AFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSC
Sbjct: 301 AFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSC 360

Query: 361 ATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRANK 420
           ATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRANK
Sbjct: 361 ATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRANK 420

Query: 421 KFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLV 480
           KFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLV
Sbjct: 421 KFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLV 480

Query: 481 PKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQF 540
           PKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQF
Sbjct: 481 PKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQF 540

Query: 541 VTGIDNIRDAIPFPRSPGSAEF 563
           VTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 VTGIDNIRDAIPFPRSPGSAEF 562

BLAST of CSPI03G02680 vs. ExPASy TrEMBL
Match: A0A1S3BLJ5 (Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103490884 PE=3 SV=1)

HSP 1 Score: 1120.9 bits (2898), Expect = 0.0e+00
Identity = 550/562 (97.86%), Postives = 555/562 (98.75%), Query Frame = 0

Query: 1   MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPIFPRHSSCYFRRSFCSAVSAGEA 60
           MAAVISPATSLRFNPCSTLRFI+GLPRKL HHILFPPI  RHSS YFRRSFCSAV AGEA
Sbjct: 1   MAAVISPATSLRFNPCSTLRFITGLPRKLSHHILFPPISRRHSSYYFRRSFCSAVPAGEA 60

Query: 61  AEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVVMGWVRTLRVQS 120
           AEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVV GWVRTLRVQS
Sbjct: 61  AEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVVKGWVRTLRVQS 120

Query: 121 SVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQKVELK 180
           SVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQKVELK
Sbjct: 121 SVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQKVELK 180

Query: 181 LQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQEN 240
           LQKV++VGKSDPS+PVQKKKVSREFLRTKAHLRPRTNTFGAVARVRN LAYATHKFFQEN
Sbjct: 181 LQKVVMVGKSDPSYPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKFFQEN 240

Query: 241 GFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFIDWSQDFFGKP 300
           GFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFIDWSQDFFGKP
Sbjct: 241 GFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFIDWSQDFFGKP 300

Query: 301 AFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSC 360
           AFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSC
Sbjct: 301 AFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSC 360

Query: 361 ATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRANK 420
           ATAYLQYVVRH+LENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRANK
Sbjct: 361 ATAYLQYVVRHVLENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRANK 420

Query: 421 KFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLV 480
           KFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLV
Sbjct: 421 KFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLV 480

Query: 481 PKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQF 540
           PKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQF
Sbjct: 481 PKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQF 540

Query: 541 VTGIDNIRDAIPFPRSPGSAEF 563
           VTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 VTGIDNIRDAIPFPRSPGSAEF 562

BLAST of CSPI03G02680 vs. ExPASy TrEMBL
Match: A0A6J1DJ47 (Asparagine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111021381 PE=3 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 1.9e-301
Identity = 510/565 (90.27%), Postives = 537/565 (95.04%), Query Frame = 0

Query: 1   MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPIFPRHSSCYFRRSF---CSAVSA 60
           MAA +SPA SLRFNP STLRFI+G PRKLP  I  PPI  RHSS  FRRSF    +A+S+
Sbjct: 1   MAAAMSPAISLRFNPYSTLRFIAGFPRKLPQSIPLPPITRRHSSGSFRRSFRAVSAALSS 60

Query: 61  GEAAEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVVMGWVRTLR 120
           GEA EKTKP+VSKDWKVTRGEKVGEFRKKLR+SEIKGG DEGLDR+GQT VV GWVRTLR
Sbjct: 61  GEAVEKTKPEVSKDWKVTRGEKVGEFRKKLRVSEIKGGLDEGLDRVGQTLVVKGWVRTLR 120

Query: 121 VQSSVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQKV 180
            QSSVTFMEVNDGSCISNMQCV+DSTTEGYDQVESGLITTGASVWV GVVVASQGSKQKV
Sbjct: 121 FQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLITTGASVWVHGVVVASQGSKQKV 180

Query: 181 ELKLQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFF 240
           ELKLQKV++VG+SDPS+P+QKK+VSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFF
Sbjct: 181 ELKLQKVVMVGESDPSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFF 240

Query: 241 QENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFIDWSQDFF 300
           QENGFVW+SSPIITASDCEGAGEQFCVTTLIP SGE A+SDVG+IPKTKEGFIDWSQDFF
Sbjct: 241 QENGFVWISSPIITASDCEGAGEQFCVTTLIPGSGEAANSDVGAIPKTKEGFIDWSQDFF 300

Query: 301 GKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDD 360
           GKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDD
Sbjct: 301 GKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDD 360

Query: 361 MSCATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLR 420
           M+CATAYLQYVVRH+LENCKEDLDFFNTWIEKG+IDRL NLVE DFVQL YTDAIELLLR
Sbjct: 361 MACATAYLQYVVRHVLENCKEDLDFFNTWIEKGVIDRLNNLVEKDFVQLTYTDAIELLLR 420

Query: 421 ANKKFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMD 480
           ANKKFE+PVKWGSDLQSEHERYITE AFGGCPVIIRDYP++IKAFYMRQNDDGKTVAAMD
Sbjct: 421 ANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAMD 480

Query: 481 MLVPKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFERL 540
           MLVP+IGELIGGSQREERLDYLEARLDDLKLNK+S+WWYLDLRRYGSVPHAGFGLGFERL
Sbjct: 481 MLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSFWWYLDLRRYGSVPHAGFGLGFERL 540

Query: 541 VQFVTGIDNIRDAIPFPRSPGSAEF 563
           VQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 VQFVTGIDNIRDAIPFPRSPGSAEF 565

BLAST of CSPI03G02680 vs. ExPASy TrEMBL
Match: A0A6J1G9C9 (Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111451973 PE=3 SV=1)

HSP 1 Score: 1040.8 bits (2690), Expect = 2.1e-300
Identity = 511/566 (90.28%), Postives = 536/566 (94.70%), Query Frame = 0

Query: 1   MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPIFPRHSSCYFRRSFCSAVSA--- 60
           MAA +SPA SLRFNP S LRFI+  P+KLP HILFPPI  RHSS YFRRSF +AVSA   
Sbjct: 1   MAAAMSPAVSLRFNPYSALRFITRFPKKLPPHILFPPISRRHSSDYFRRSFRTAVSAALS 60

Query: 61  -GEAAEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVVMGWVRTL 120
            GEA  KTKP+VS  WK + GEKVGEFRKKLRISE+KGG DEGLDR+GQT +V GWVRTL
Sbjct: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCV+DSTTEGYDQVESGLI TGASVWV+GVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180

Query: 181 VELKLQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240
           VELKLQKV++VGKSDPS+PVQKK+VSREFLRTKAHLRPRTNTFGAVARVRN LAYATHKF
Sbjct: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGE A+SDVGSIPKTKEGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSDVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMSCATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLL 420
           DM+CATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLT+LVE DFVQL YTDA+ELLL
Sbjct: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420

Query: 421 RANKKFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAM 480
           RANKKFE+PVKWGSDLQSEHERYITE AFGGCPVIIRDYP++IKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVP+IGELIGGSQREERLDYLEARLD+LKLNK+SYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 563
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566

BLAST of CSPI03G02680 vs. ExPASy TrEMBL
Match: A0A6J1L1Y2 (Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111498374 PE=3 SV=1)

HSP 1 Score: 1036.2 bits (2678), Expect = 5.1e-299
Identity = 509/566 (89.93%), Postives = 536/566 (94.70%), Query Frame = 0

Query: 1   MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPIFPRHSSCYFRRSFCSAVSA--- 60
           MAA +SPA SLRFNP S LRFI+  P+KLP +ILFPPI  RHSS YFRRSF +AVSA   
Sbjct: 1   MAAAMSPAVSLRFNPYSALRFITRFPKKLPPYILFPPIPRRHSSDYFRRSFRTAVSAALS 60

Query: 61  -GEAAEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVVMGWVRTL 120
            GEA  KTKP+VS  WK + GEKVGEFRKKLRISE+KGG DEGLDR+GQT +V GWVRTL
Sbjct: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCV+DSTTEGYDQVESGLI TGASVWV+GVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180

Query: 181 VELKLQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240
           VELKLQKV++VGKSDPS+PVQKK+VSREFLRTKAHLRPRTNTFGAVARVRN LAYATHKF
Sbjct: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGE A+SDVGSIPKTKEGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSDVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMSCATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLL 420
           DM+CATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLT+LVE DFVQL YTDA++LLL
Sbjct: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVKLLL 420

Query: 421 RANKKFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAM 480
           RANKKFE+PVKWGSDLQSEHERYITE AFGGCPVIIRDYP++IKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVP+IGELIGGSQREERLDYLEARLD+LKLNK+SYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 563
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566

BLAST of CSPI03G02680 vs. NCBI nr
Match: XP_004147209.1 (asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN55862.1 hypothetical protein Csa_009714 [Cucumis sativus])

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 560/562 (99.64%), Postives = 561/562 (99.82%), Query Frame = 0

Query: 1   MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPIFPRHSSCYFRRSFCSAVSAGEA 60
           MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPIFPRHSSCYFRRSFCSAVSAGEA
Sbjct: 1   MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPIFPRHSSCYFRRSFCSAVSAGEA 60

Query: 61  AEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVVMGWVRTLRVQS 120
           AEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEG+DRLGQTFVVMGWVRTLRVQS
Sbjct: 61  AEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGVDRLGQTFVVMGWVRTLRVQS 120

Query: 121 SVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQKVELK 180
           SVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQKVELK
Sbjct: 121 SVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQKVELK 180

Query: 181 LQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQEN 240
           LQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQEN
Sbjct: 181 LQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQEN 240

Query: 241 GFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFIDWSQDFFGKP 300
           GFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPA SDVGSIPKTKEGFIDWSQDFFGKP
Sbjct: 241 GFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPARSDVGSIPKTKEGFIDWSQDFFGKP 300

Query: 301 AFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSC 360
           AFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSC
Sbjct: 301 AFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSC 360

Query: 361 ATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRANK 420
           ATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRANK
Sbjct: 361 ATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRANK 420

Query: 421 KFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLV 480
           KFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLV
Sbjct: 421 KFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLV 480

Query: 481 PKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQF 540
           PKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQF
Sbjct: 481 PKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQF 540

Query: 541 VTGIDNIRDAIPFPRSPGSAEF 563
           VTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 VTGIDNIRDAIPFPRSPGSAEF 562

BLAST of CSPI03G02680 vs. NCBI nr
Match: XP_008448845.1 (PREDICTED: asparagine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 1120.9 bits (2898), Expect = 0.0e+00
Identity = 550/562 (97.86%), Postives = 555/562 (98.75%), Query Frame = 0

Query: 1   MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPIFPRHSSCYFRRSFCSAVSAGEA 60
           MAAVISPATSLRFNPCSTLRFI+GLPRKL HHILFPPI  RHSS YFRRSFCSAV AGEA
Sbjct: 1   MAAVISPATSLRFNPCSTLRFITGLPRKLSHHILFPPISRRHSSYYFRRSFCSAVPAGEA 60

Query: 61  AEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVVMGWVRTLRVQS 120
           AEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVV GWVRTLRVQS
Sbjct: 61  AEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVVKGWVRTLRVQS 120

Query: 121 SVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQKVELK 180
           SVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQKVELK
Sbjct: 121 SVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQKVELK 180

Query: 181 LQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQEN 240
           LQKV++VGKSDPS+PVQKKKVSREFLRTKAHLRPRTNTFGAVARVRN LAYATHKFFQEN
Sbjct: 181 LQKVVMVGKSDPSYPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKFFQEN 240

Query: 241 GFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFIDWSQDFFGKP 300
           GFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFIDWSQDFFGKP
Sbjct: 241 GFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFIDWSQDFFGKP 300

Query: 301 AFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSC 360
           AFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSC
Sbjct: 301 AFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSC 360

Query: 361 ATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRANK 420
           ATAYLQYVVRH+LENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRANK
Sbjct: 361 ATAYLQYVVRHVLENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRANK 420

Query: 421 KFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLV 480
           KFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLV
Sbjct: 421 KFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLV 480

Query: 481 PKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQF 540
           PKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQF
Sbjct: 481 PKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQF 540

Query: 541 VTGIDNIRDAIPFPRSPGSAEF 563
           VTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 VTGIDNIRDAIPFPRSPGSAEF 562

BLAST of CSPI03G02680 vs. NCBI nr
Match: XP_038904123.1 (asparagine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 1093.2 bits (2826), Expect = 0.0e+00
Identity = 534/562 (95.02%), Postives = 550/562 (97.86%), Query Frame = 0

Query: 1   MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPIFPRHSSCYFRRSFCSAVSAGEA 60
           MAA +SPATSLRFNPCSTLRFI+GLPRKLP HILFPP+  RHSS YFRR+FC+AVS+GEA
Sbjct: 1   MAAAMSPATSLRFNPCSTLRFITGLPRKLPQHILFPPVSRRHSSGYFRRTFCTAVSSGEA 60

Query: 61  AEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVVMGWVRTLRVQS 120
           AEKTKP+VSKDWKVTRGEKVGEFRKKLRISEIKGG DEGLDRLGQTFVV GWVRTLRVQS
Sbjct: 61  AEKTKPEVSKDWKVTRGEKVGEFRKKLRISEIKGGLDEGLDRLGQTFVVKGWVRTLRVQS 120

Query: 121 SVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQKVELK 180
           SVTFMEVNDGSCISNMQCVIDST EGYDQVESGLITTGASVWV+GVVVASQGSKQKVELK
Sbjct: 121 SVTFMEVNDGSCISNMQCVIDSTIEGYDQVESGLITTGASVWVQGVVVASQGSKQKVELK 180

Query: 181 LQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQEN 240
           LQK++VVGKSDPS+PVQKK+VS+EFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQEN
Sbjct: 181 LQKLVVVGKSDPSYPVQKKRVSKEFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQEN 240

Query: 241 GFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFIDWSQDFFGKP 300
           GFVWVSSPIITASDCEGAGEQFCVTTLIPSS E ASSDVGSIPKTKEGFIDWSQDFFGKP
Sbjct: 241 GFVWVSSPIITASDCEGAGEQFCVTTLIPSSEEAASSDVGSIPKTKEGFIDWSQDFFGKP 300

Query: 301 AFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSC 360
           AFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSC
Sbjct: 301 AFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSC 360

Query: 361 ATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRANK 420
           ATAYLQYVVRHILENCKED+DFFNTWIEKGIIDRLTNLVENDFVQL YTDAIELLLRANK
Sbjct: 361 ATAYLQYVVRHILENCKEDMDFFNTWIEKGIIDRLTNLVENDFVQLSYTDAIELLLRANK 420

Query: 421 KFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLV 480
           KFEYPVKWGSDLQSEHERYITE AFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLV
Sbjct: 421 KFEYPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLV 480

Query: 481 PKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQF 540
           P+IGELIGGSQREERLDYLEARLDDLKLNK+SYWWYLDLRRYGSVPHAGFGLGFERLVQF
Sbjct: 481 PRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAGFGLGFERLVQF 540

Query: 541 VTGIDNIRDAIPFPRSPGSAEF 563
           VTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 VTGIDNIRDAIPFPRSPGSAEF 562

BLAST of CSPI03G02680 vs. NCBI nr
Match: XP_022154023.1 (asparagine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia])

HSP 1 Score: 1044.3 bits (2699), Expect = 3.9e-301
Identity = 510/565 (90.27%), Postives = 537/565 (95.04%), Query Frame = 0

Query: 1   MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPIFPRHSSCYFRRSF---CSAVSA 60
           MAA +SPA SLRFNP STLRFI+G PRKLP  I  PPI  RHSS  FRRSF    +A+S+
Sbjct: 1   MAAAMSPAISLRFNPYSTLRFIAGFPRKLPQSIPLPPITRRHSSGSFRRSFRAVSAALSS 60

Query: 61  GEAAEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVVMGWVRTLR 120
           GEA EKTKP+VSKDWKVTRGEKVGEFRKKLR+SEIKGG DEGLDR+GQT VV GWVRTLR
Sbjct: 61  GEAVEKTKPEVSKDWKVTRGEKVGEFRKKLRVSEIKGGLDEGLDRVGQTLVVKGWVRTLR 120

Query: 121 VQSSVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQKV 180
            QSSVTFMEVNDGSCISNMQCV+DSTTEGYDQVESGLITTGASVWV GVVVASQGSKQKV
Sbjct: 121 FQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLITTGASVWVHGVVVASQGSKQKV 180

Query: 181 ELKLQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFF 240
           ELKLQKV++VG+SDPS+P+QKK+VSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFF
Sbjct: 181 ELKLQKVVMVGESDPSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFF 240

Query: 241 QENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFIDWSQDFF 300
           QENGFVW+SSPIITASDCEGAGEQFCVTTLIP SGE A+SDVG+IPKTKEGFIDWSQDFF
Sbjct: 241 QENGFVWISSPIITASDCEGAGEQFCVTTLIPGSGEAANSDVGAIPKTKEGFIDWSQDFF 300

Query: 301 GKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDD 360
           GKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDD
Sbjct: 301 GKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDD 360

Query: 361 MSCATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLR 420
           M+CATAYLQYVVRH+LENCKEDLDFFNTWIEKG+IDRL NLVE DFVQL YTDAIELLLR
Sbjct: 361 MACATAYLQYVVRHVLENCKEDLDFFNTWIEKGVIDRLNNLVEKDFVQLTYTDAIELLLR 420

Query: 421 ANKKFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMD 480
           ANKKFE+PVKWGSDLQSEHERYITE AFGGCPVIIRDYP++IKAFYMRQNDDGKTVAAMD
Sbjct: 421 ANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAMD 480

Query: 481 MLVPKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFERL 540
           MLVP+IGELIGGSQREERLDYLEARLDDLKLNK+S+WWYLDLRRYGSVPHAGFGLGFERL
Sbjct: 481 MLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSFWWYLDLRRYGSVPHAGFGLGFERL 540

Query: 541 VQFVTGIDNIRDAIPFPRSPGSAEF 563
           VQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 VQFVTGIDNIRDAIPFPRSPGSAEF 565

BLAST of CSPI03G02680 vs. NCBI nr
Match: XP_022948254.1 (asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata] >KAG6596861.1 Asparagine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028390.1 Asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1040.8 bits (2690), Expect = 4.3e-300
Identity = 511/566 (90.28%), Postives = 536/566 (94.70%), Query Frame = 0

Query: 1   MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPIFPRHSSCYFRRSFCSAVSA--- 60
           MAA +SPA SLRFNP S LRFI+  P+KLP HILFPPI  RHSS YFRRSF +AVSA   
Sbjct: 1   MAAAMSPAVSLRFNPYSALRFITRFPKKLPPHILFPPISRRHSSDYFRRSFRTAVSAALS 60

Query: 61  -GEAAEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVVMGWVRTL 120
            GEA  KTKP+VS  WK + GEKVGEFRKKLRISE+KGG DEGLDR+GQT +V GWVRTL
Sbjct: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCV+DSTTEGYDQVESGLI TGASVWV+GVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180

Query: 181 VELKLQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240
           VELKLQKV++VGKSDPS+PVQKK+VSREFLRTKAHLRPRTNTFGAVARVRN LAYATHKF
Sbjct: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGE A+SDVGSIPKTKEGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSDVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMSCATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLL 420
           DM+CATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLT+LVE DFVQL YTDA+ELLL
Sbjct: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420

Query: 421 RANKKFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAM 480
           RANKKFE+PVKWGSDLQSEHERYITE AFGGCPVIIRDYP++IKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVP+IGELIGGSQREERLDYLEARLD+LKLNK+SYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 563
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566

BLAST of CSPI03G02680 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 874.8 bits (2259), Expect = 3.8e-254
Identity = 432/572 (75.52%), Postives = 491/572 (85.84%), Query Frame = 0

Query: 1   MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPI----------FPRHSSCYFRRS 60
           MAA   PATSLR    STLRF+S      P + LF P+          FP +S    RR 
Sbjct: 1   MAATFLPATSLRLTQNSTLRFLSFFTISNPSYSLFRPLRRRVLPPFDAFPANSR---RRC 60

Query: 61  FCSAVSAGEAAEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVVM 120
           FC+AVS  E+      +  + ++   G KVGEFRKKLRI+E+KGG DEGL R+GQ+  +M
Sbjct: 61  FCTAVS--ESLGSGDGNKVESYEKRFGSKVGEFRKKLRIAEVKGGADEGLSRVGQSLNIM 120

Query: 121 GWVRTLRVQSSVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVAS 180
           GWVRTLR QSSVTF+E+NDGSC+SN+QCV+ S  EGYDQVESG I TGASV V+G +VAS
Sbjct: 121 GWVRTLRSQSSVTFIEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGASVSVQGTIVAS 180

Query: 181 QGSKQKVELKLQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALA 240
           QG+KQKVELK++K+IVVG+ D S+P+QKK+VSREFLRTKAHLRPRTNTFGAVARVRN LA
Sbjct: 181 QGTKQKVELKVEKIIVVGECDSSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTLA 240

Query: 241 YATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFI 300
           YATHKFFQE+GFVWV+SPIITASDCEGAGEQFCVTTLIPSS E   + + +IPKTK G I
Sbjct: 241 YATHKFFQESGFVWVASPIITASDCEGAGEQFCVTTLIPSSHENTDTSIDAIPKTKGGLI 300

Query: 301 DWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELA 360
           DWSQDFFGKPAFLTVSGQLN ETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELA
Sbjct: 301 DWSQDFFGKPAFLTVSGQLNGETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELA 360

Query: 361 FADLDDDMSCATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTD 420
           FADLDDDM+CATAYLQYVV+++L+NCKED++FF+TWIEKGII RL+++ E +F+QL YTD
Sbjct: 361 FADLDDDMACATAYLQYVVKYVLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTD 420

Query: 421 AIELLLRANKKFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDG 480
           AIE+LL+ANKKF++PVKWG DLQSEHERYITE AFGG PVIIRDYP++IKAFYMR+NDDG
Sbjct: 421 AIEILLKANKKFDFPVKWGLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDDG 480

Query: 481 KTVAAMDMLVPKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGF 540
           KTVAAMDMLVP+IGELIGGSQREERL+ LEARLD+LKLNKESYWWYLDLRRYGSVPHAGF
Sbjct: 481 KTVAAMDMLVPRIGELIGGSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGF 540

Query: 541 GLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 563
           GLGFERLVQFVTGIDNIRD IPFPR+P SAEF
Sbjct: 541 GLGFERLVQFVTGIDNIRDVIPFPRTPASAEF 567

BLAST of CSPI03G02680 vs. TAIR 10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 498.8 bits (1283), Expect = 5.6e-141
Identity = 270/550 (49.09%), Postives = 352/550 (64.00%), Query Frame = 0

Query: 82  EFRKKLRISEIKGGRDEGLDRLGQTFVVMGWVRTLRVQSSVT--FMEVNDGSCISNMQCV 141
           +F  ++ I  I GG   G    GQ   + GWV+T R Q   T  F+EVNDGSC +N+Q +
Sbjct: 28  QFSDRVLIRSILGG---GAKLAGQKVRIGGWVKTGRQQGKGTFAFLEVNDGSCPANLQVM 87

Query: 142 IDSTTEGYDQVESGLITTGASVWVEGVV---VASQGSKQKVELKLQKVIVVGKSDP-SFP 201
           +DS+   YD   S L+ TG  V V+GV+      +G KQ +EL ++ VI VG  DP ++P
Sbjct: 88  VDSSL--YDL--SRLVATGTCVTVDGVLKIPPEGKGLKQSIELSVETVIAVGTVDPTTYP 147

Query: 202 VQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQENGFVWVSSPIITASDC 261
           + K K++ EFLR   HLR RTN   AVAR+RNALA+ATH FFQE+ F+++ +PIIT SDC
Sbjct: 148 LPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDC 207

Query: 262 EGAGEQFCVTTLI-----------------------------------------PSSGEP 321
           EGAGE F VTTLI                                          +S E 
Sbjct: 208 EGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVEAARLIVKERGEAVAQLKVAKASKEE 267

Query: 322 ASSDVGSIPKTK-------------------EGFIDWSQDFFGKPAFLTVSGQLNAETYA 381
            ++ V  +   K                   +G ID+S DFFG+ AFLTVSGQL  ETYA
Sbjct: 268 ITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKIDYSNDFFGRQAFLTVSGQLQVETYA 327

Query: 382 TALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSCATAYLQYVVRHILEN 441
            ALS VYTFGPTFRAENS+TSRHLAEFWM+EPE+AFAD+ DDM+CA AY++Y+ + +++ 
Sbjct: 328 CALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCAEAYVKYMCKWLMDK 387

Query: 442 CKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRANKK----FEYPVKWGSD 501
           C +D++  +  +++G   RL  + +  F ++ YT+AIE L +A  +    F+  V+WG D
Sbjct: 388 CGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTEAIERLEKAVAQGKVVFDNKVEWGID 447

Query: 502 LQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLVPKIGELIGGSQ 561
           L SEHERY+TEV F   P+I+ +YP+ IKAFYMR NDD KTVAAMD+LVPK+GELIGGSQ
Sbjct: 448 LASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQ 507

BLAST of CSPI03G02680 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 490.3 bits (1261), Expect = 2.0e-138
Identity = 272/550 (49.45%), Postives = 350/550 (63.64%), Query Frame = 0

Query: 82  EFRKKLRISEIKGGRDEGLDRLGQTFVVMGWVRTLRVQSSVT--FMEVNDGSCISNMQCV 141
           +F  ++ I  I    D G    GQT  + GWV++ R Q   T  F+ VNDGSC +N+Q +
Sbjct: 28  QFSNRVLIRTILDRPDGGAGLAGQTVRIGGWVKSGRDQGKRTFSFLAVNDGSCPANLQVM 87

Query: 142 IDSTTEGYDQVESGLITTGASVWVEGVVVA---SQGSKQKVELKLQKVIVVGKSDPS-FP 201
           +D +   YD   S L+ TG  V V+GV+      +G++Q++EL + KVI VG  D S +P
Sbjct: 88  VDPSL--YD--VSNLVATGTCVTVDGVLKVPPKGKGTQQQIELNVVKVIDVGTVDASKYP 147

Query: 202 VQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQENGFVWVSSPIITASDC 261
           + K K++ E LR   HLR RTN+  AVAR+RNALA+ATH FFQE+ F+++ +PIIT SDC
Sbjct: 148 LPKTKLTLETLRDVLHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDC 207

Query: 262 EGAGEQFCVTTLIP----------SSGEPASSDVGS------------------------ 321
           EGAGE F  TTLI            +  P  +DV +                        
Sbjct: 208 EGAGEMFQATTLINYTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKASKEA 267

Query: 322 ---------------------------IPKTKEGFIDWSQDFFGKPAFLTVSGQLNAETY 381
                                      +PK K+G ID+S+DFFG+ AFLTVSGQL  ETY
Sbjct: 268 ITAAVAELKIAKETFAHIDERSRLRPGLPK-KDGNIDYSKDFFGRQAFLTVSGQLQVETY 327

Query: 382 ATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMSCATAYLQYVVRHILE 441
           A ALS+VYTFGPTFRAENS+TSRHLAEFWM+EPE+AFADL+DDM+CA AY++Y+   +LE
Sbjct: 328 ACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNWLLE 387

Query: 442 NCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRA---NKKFEYPVKWGSD 501
            C  D++      + G IDRL  +    F ++ YT AIELL  A    K+F+  V+WG D
Sbjct: 388 KCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAIELLEEAVAKGKEFDNNVEWGID 447

Query: 502 LQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMDMLVPKIGELIGGSQ 561
           L SEHERY+TEV F   P+I+ +YP+ IKAFYMR NDD KTVAAMD+LVPK+GELIGGSQ
Sbjct: 448 LASEHERYLTEVLFQK-PLIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQ 507

BLAST of CSPI03G02680 vs. TAIR 10
Match: AT3G07420.1 (asparaginyl-tRNA synthetase 2 )

HSP 1 Score: 339.3 bits (869), Expect = 5.7e-93
Identity = 192/503 (38.17%), Postives = 283/503 (56.26%), Query Frame = 0

Query: 122 VTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGV--VVASQGSKQKVEL 181
           V +  ++DGS IS++Q V+DS        +  L+  G  +  EGV  +  +  +K  +EL
Sbjct: 139 VFYFLISDGSSISSLQVVVDSALSTVPATQ--LMALGTCIVAEGVLRLPLAASAKHVIEL 198

Query: 182 KLQKVIVVGKSDP-SFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQ 241
           + +K++ VG  DP  +P+ KK++    LR  +H RPRT T G+V RV +AL  A+H F Q
Sbjct: 199 EAEKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTFLQ 258

Query: 242 ENGFVWVSSPIITASDCEGAGEQFCVTTLI------------------------------ 301
            +GF +V  P+IT +   G GE F VTTL+                              
Sbjct: 259 YHGFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEEKKPPVQEKDGFSIDTVKAVIKE 318

Query: 302 -----------PSSGEPASSDVGSIPKT-------------------KEGFIDWSQDFFG 361
                       S+ E   + V  + KT                   K   +D+S+DFFG
Sbjct: 319 KTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFSKDFFG 378

Query: 362 KPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDM 421
           +  +LT SG+ + E+YA+AL  VYTFGP F A+  + +RHLAE W +E E+AFA+LDD M
Sbjct: 379 RDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAFAELDDAM 438

Query: 422 SCATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLLRA 481
            CA  Y +++ +++LEN  ED+ F +  ++K I  RL     +  ++  YT+ I LL +A
Sbjct: 439 DCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEVISLLQKA 498

Query: 482 -NKKFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAMD 541
              KFE   +WG  L +EH  Y+T+  + G PVI+  YP+ IK FY+R NDD KTVAA D
Sbjct: 499 TTTKFETKPEWGVALTTEHLSYLTDEIYKG-PVIVHTYPKAIKQFYVRLNDDKKTVAAFD 558

Query: 542 MLVPKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFERL 561
           ++VPK+G +I GSQ EER + L+AR+ +    +E + WYLDLRR+G+V H+G  L  E++
Sbjct: 559 LVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHSGISLSMEQM 618

BLAST of CSPI03G02680 vs. TAIR 10
Match: AT4G26870.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 118.6 bits (296), Expect = 1.6e-26
Identity = 110/452 (24.34%), Postives = 201/452 (44.47%), Query Frame = 0

Query: 136 MQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQ------GSKQKVELKLQKVIVVGK 195
           +QCV++ T  G + ++     +  SV VE + V S       G+ Q+VE+ ++K+  + +
Sbjct: 115 VQCVVEETRVGANMIKFVKQLSRESV-VELIGVVSHPKKPLTGTTQQVEIHVRKMYCLSR 174

Query: 196 SDPSFPV-----------------QKKKVSREFLRTKAHLRP---RTNTFGAVARVRNAL 255
           S P+ P+                   K+ +R    T+ + R    RT    A+ R++  +
Sbjct: 175 SLPNLPLVVEDAARSESDIEKSGKDGKQAARVLQDTRLNNRVLDIRTPANQAIFRIQCQV 234

Query: 256 AYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGF 315
             A  ++ Q  GF+ + +P + A   EG    F +                         
Sbjct: 235 QIAFREYLQSKGFLEIHTPKLIAGSSEGGSAVFRL------------------------- 294

Query: 316 IDWSQDFFGKPAFLTVSGQLNAE-TYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPE 375
                D+ G+PA L  S QL+ +      +  V+  GP FRAE+S T RHL EF  ++ E
Sbjct: 295 -----DYKGQPACLAQSPQLHKQMAICGDMRRVFEVGPVFRAEDSFTHRHLCEFVGLDVE 354

Query: 376 LAF-ADLDDDMSCATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLP 435
           +       + M        ++   I E C ++L+      ++     L  L +   ++L 
Sbjct: 355 MEIRMHYSEIMDLVGELFPFIFTKIEERCPKELESVR---KQYPFQSLKFLPQT--LRLT 414

Query: 436 YTDAIELLLRANKKFEYPVKWGSDLQSEHERYITEVAFGGCPV---IIRDYPRDIKAFY- 495
           + + I++L  A ++ + P+    DL +E ER + ++          ++  YP  ++ FY 
Sbjct: 415 FAEGIQMLKEAGEEVD-PL---GDLNTESERKLGQLVLEKYKTEFYMLHRYPSAVRPFYT 474

Query: 496 MRQNDDGKTVAAMDMLVPKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYG 555
           M   +D     + D+ + +  E++ G+QR    + LE R  +  ++ ++   Y+D  RYG
Sbjct: 475 MPYENDSNYSNSFDVFI-RGEEIMSGAQRIHDPELLEKRARECGIDVKTISTYIDAFRYG 525

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O485935.3e-25375.52Asparagine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=... [more]
Q6MEC92.7e-16457.95Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 G... [more]
P586923.8e-15860.25Asparagine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) ... [more]
P522763.2e-15760.53Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11117... [more]
Q899M97.9e-15657.39Asparagine--tRNA ligase OS=Clostridium tetani (strain Massachusetts / E88) OX=21... [more]
Match NameE-valueIdentityDescription
A0A0A0L5I10.0e+0099.64Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_3G020020 PE=3 SV=1[more]
A0A1S3BLJ50.0e+0097.86Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103490884 PE=3 SV=1[more]
A0A6J1DJ471.9e-30190.27Asparagine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111021381 PE=3 SV=1[more]
A0A6J1G9C92.1e-30090.28Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111451973 PE=3 SV=1[more]
A0A6J1L1Y25.1e-29989.93Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111498374 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004147209.10.0e+0099.64asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN55862... [more]
XP_008448845.10.0e+0097.86PREDICTED: asparagine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucu... [more]
XP_038904123.10.0e+0095.02asparagine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Benincasa hispi... [more]
XP_022154023.13.9e-30190.27asparagine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia][more]
XP_022948254.14.3e-30090.28asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata] >KAG65... [more]
Match NameE-valueIdentityDescription
AT4G17300.13.8e-25475.52Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT1G70980.15.6e-14149.09Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT5G56680.12.0e-13849.45Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT3G07420.15.7e-9338.17asparaginyl-tRNA synthetase 2 [more]
AT4G26870.11.6e-2624.34Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002312Aspartyl/Asparaginyl-tRNA synthetase, class IIbPRINTSPR01042TRNASYNTHASPcoord: 303..315
score: 41.78
coord: 319..332
score: 44.32
coord: 477..493
score: 44.65
coord: 517..531
score: 54.7
IPR004522Asparagine-tRNA ligaseTIGRFAMTIGR00457TIGR00457coord: 103..562
e-value: 3.6E-186
score: 617.3
IPR004522Asparagine-tRNA ligaseHAMAPMF_00534Asn_tRNA_synthcoord: 92..562
score: 44.253975
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 203..556
e-value: 1.6E-78
score: 264.0
NoneNo IPR availableGENE3D2.40.50.140coord: 82..192
e-value: 2.9E-16
score: 61.2
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 195..560
e-value: 8.1E-105
score: 352.9
NoneNo IPR availablePANTHERPTHR22594ASPARTYL/LYSYL-TRNA SYNTHETASEcoord: 34..562
NoneNo IPR availablePANTHERPTHR22594:SF46ASPARAGINE--TRNA LIGASE, CHLOROPLASTIC/MITOCHONDRIALcoord: 34..562
NoneNo IPR availableCDDcd00776AsxRS_corecoord: 200..558
e-value: 4.46981E-154
score: 442.005
NoneNo IPR availableCDDcd04318EcAsnRS_like_Ncoord: 106..189
e-value: 1.13346E-31
score: 115.355
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 190..560
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 224..552
score: 16.236507
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 102..191

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G02680.1CSPI03G02680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006421 asparaginyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004816 asparagine-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding